CN106489915A - Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism - Google Patents
Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism Download PDFInfo
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- CN106489915A CN106489915A CN201611236911.XA CN201611236911A CN106489915A CN 106489915 A CN106489915 A CN 106489915A CN 201611236911 A CN201611236911 A CN 201611236911A CN 106489915 A CN106489915 A CN 106489915A
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- carrier mechanism
- freezing carrier
- cellosilk
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- 238000007710 freezing Methods 0.000 title claims abstract description 110
- 230000008014 freezing Effects 0.000 title claims abstract description 110
- 230000007246 mechanism Effects 0.000 title claims abstract description 84
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000007654 immersion Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 238000005452 bending Methods 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 2
- 239000002577 cryoprotective agent Substances 0.000 abstract description 46
- 230000009471 action Effects 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000005138 cryopreservation Methods 0.000 abstract description 2
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 43
- 102000002322 Egg Proteins Human genes 0.000 description 10
- 108010000912 Egg Proteins Proteins 0.000 description 10
- 210000001161 mammalian embryo Anatomy 0.000 description 10
- 210000004681 ovum Anatomy 0.000 description 10
- 239000011521 glass Substances 0.000 description 9
- 238000005253 cladding Methods 0.000 description 8
- 239000000835 fiber Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000008602 contraction Effects 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 239000003223 protective agent Substances 0.000 description 3
- 230000002269 spontaneous effect Effects 0.000 description 3
- 238000004017 vitrification Methods 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 2
- 210000004534 cecum Anatomy 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000003566 sealing material Substances 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 229920000049 Carbon (fiber) Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004917 carbon fiber Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
- A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
- A01N1/147—Carriers for immersion in cryogenic fluid for slow freezing or vitrification
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明涉及一种冷冻载体机构和冷冻载体单元，冷冻载体机构包括固持结构和与所述固持结构连接的冻存结构，所述冻存结构包括加载区及毛细作用结构，所述加载区用于承载所述细胞，所述毛细作用结构用于产生毛细作用以吸取所述加载区的浸润液体。该冷冻载体机构通过冻存结构产生毛细作用，从而将包覆在细胞周围的大部分冷冻保护剂吸走，仅剩极少量的冷冻保护剂液膜包覆在细胞周围，该吸取过程由冷冻载体机构自动自发完成，不依赖于人工操作的控制，实现了冷冻保护剂包覆量的自动最小化，避免了技术水平不同造成的差异，从而可达到该操作标准化控制的目的，进而有效地提高细胞的存活率和复苏率，保证其发育潜能。
The invention relates to a frozen carrier mechanism and a frozen carrier unit. The frozen carrier mechanism includes a holding structure and a freezing storage structure connected to the holding structure. The freezing storage structure includes a loading area and a capillary action structure. The loading area is used for Carrying the cells, the capillary action structure is used to generate capillary action to absorb the immersion liquid of the loading area. The cryocarrier mechanism produces capillary action through the cryopreservation structure, thereby sucking away most of the cryoprotectant coated around the cells, leaving only a small amount of cryoprotectant liquid film wrapped around the cells. The mechanism is automatically completed without relying on the control of manual operation, which realizes the automatic minimization of the amount of cryoprotectant coating and avoids the differences caused by different technical levels, so as to achieve the purpose of standardized control of this operation, and then effectively improve the quality of cells. The survival rate and recovery rate are high, and their developmental potential is guaranteed.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of freezing carrier mechanism and including the freezing carrier mechanism
Freezing carrier unit.
Background technology
Refrigeration Technique is the important content in auxiliary procreation technology link, and vitrification is the ovum of current main flow
Son/zygote/Embryo Cryopreservation Technology.Vitrification is made highly concentrated by the cooling rate (2000 DEG C/min of ＞) of superelevation
The cells such as the ovum/zygote/embryo of degree cryoprotective agent cladding quickly form glassy state, it is to avoid intracellular generation ice crystal damages thin
Born of the same parents, effectively improve ovum/zygote/embryo survival, anabiosis rate, it is ensured that its potentiality of development.In order to obtain the freezing speed of maximum
Rate, cryoprotective agent volume are the smaller the better.When cryoprotective agent covering amount is larger, freezing rate can be slack-off, and less freezing
Protective agent covering amount can shorten the time of conduction of heat during freezing and rewarming.If freezing rate is slow, turning higher than vitrification
The temperature (- 140 DEG C) of change has stop, it is possible to forms intracellular ice crystal and damages ovum/zygote/embryo.
Existing freezing carrier mechanism depends greatly on the technology water of operator in control cryoprotective agent volume
Flat, need to carry out operator corresponding special training and standardized quality control before certain freezing carrier mechanism specifically used
System, but the difference between different operating person is still difficult to avoid that, even same operator, skill under different time different conditions
The performance of art level there is also difference, thus cannot realize the standardization control of the operation.
Content of the invention
It is based on this, it is necessary to cannot realize what cryoprotective agent covering amount was minimized automatically for above-mentioned freezing carrier mechanism
A kind of problem, there is provided the freezing carrier mechanism that achievable cryoprotective agent covering amount is minimized automatically.
Specific technical scheme is as follows：
A kind of freezing carrier mechanism, for carrying cell, the freezing carrier mechanism includes holding structure and solid with described
The frozen structure of structure connection is held, the frozen structure includes that loading zone and capillarity arrangements, the loading zone are used for carrying
The cell, the capillarity arrangements are used for producing capillarity to draw the immersion liquid of the loading zone.
The cell for being coated with cryoprotective agent can be transferred to adding for frozen structure when using by above-mentioned freezing carrier mechanism
Area is carried, and capillarity is produced by capillarity arrangements, is siphoned away so as to be coated on pericellular most of cryoprotective agent,
Only remain minimal amount of cryoprotective agent liquid film and be coated on cell peripheral, the suction process is automatic and spontaneous complete by freezing carrier mechanism
Into not relying on manually-operated control, it is achieved that the automatic minimum of cryoprotective agent covering amount, it is to avoid technical merit is not
With the difference for causing, so as to can reach the purpose of the operational standardization control, and then ovum/zygote/embryo etc. is effectively improved
The survival rate of cell and anabiosis rate, it is ensured that its potentiality of development.
Wherein in one embodiment, the capillarity arrangements are provided with for producing capillary opening or gap,
The loading zone is located at the chi in the region that the capillarity arrangements are provided with the opening or the gap, the opening or gap
The very little diameter less than the cell.
Wherein in one embodiment, the capillarity arrangements are tubular structure.
Wherein in one embodiment, at least there are the capillarity arrangements two relative sides, the capillary to make
Dead slot is offered with least one side of structure, so that the gap between described two relative sides forms the gap.
Wherein in one embodiment, the capillarity arrangements include extension and the company for being connected to the holding structure
Bending section of the extension away from described holding structure one end is connected to, the bending section bending extension, the bending section do not connect
The end face for connecing the extension is provided with the opening, and with the extension interval setting and spacing distance is less than the cell
Diameter.
Wherein in one embodiment, the capillarity arrangements include cellosilk and connecting tube, described filametntary extremely
Few one end is stretched in the connecting tube, so as to form the gap between the tube wall of the cellosilk and the connecting tube.
Wherein in one embodiment, the filametntary quantity is at least two, and described at least two, cellosilk is parallel
And contact with each other.
Wherein in one embodiment, the capillarity arrangements be at least two cellosilks, fiber described at least two
The gap is formed between silk, described cellosilk one end is connected with the holding structure.
Wherein in one embodiment, the frozen structure also includes absorb water bar, the water suction bar and the holding structure
Connection, the cellosilk are connected to be connected with the holding structure by the water suction bar with the water suction bar.
Another object of the present invention is to a kind of freezing carrier unit is provided, including described freezing carrier mechanism and set
Pipe, the freezing carrier mechanism are contained in described sleeve pipe and can be along described sleeve pipe axially-movables, and described sleeve pipe is provided with axial limit
Bit architecture is limiting the ultimate range that the freezing carrier mechanism moves to the frozen structure place direction.
Description of the drawings
Structural representations of the Fig. 1 for the freezing carrier mechanism of first embodiment；
Structural representations of the Fig. 2 for the freezing carrier mechanism of second embodiment；
Structural representations of the Fig. 3 for frozen structure in the freezing carrier mechanism shown in Fig. 1；
Fig. 4 is the partial enlarged drawing in Fig. 3 at A；
Structural representations of the Fig. 5 for frozen structure in the freezing carrier mechanism of 3rd embodiment；
Fig. 6 is the partial enlarged drawing in Fig. 5 at B；
Structural representations of the Fig. 7 for frozen structure in the freezing carrier mechanism of fourth embodiment；
Fig. 8 is the partial enlarged drawing in Fig. 7 at C；
Fig. 9 be the 5th embodiment freezing carrier mechanism in frozen structure structural representation；
Figure 10 is the partial enlarged drawing in Fig. 9 at D；
Structural representations of the Figure 11 for frozen structure in the freezing carrier mechanism of sixth embodiment；
Figure 12 is the partial enlarged drawing in Figure 11 at E；
Figure 13 be the 7th embodiment freezing carrier mechanism in frozen structure structural representation；
Figure 14 is the partial enlarged drawing in Figure 13 at F；
Figure 15 be the 8th embodiment freezing carrier mechanism in frozen structure structural representation；
Figure 16 is the partial enlarged drawing in Figure 15 at G；
Figure 17 be the 9th embodiment freezing carrier mechanism in frozen structure structural representation；
Figure 18 is the partial enlarged drawing in Figure 17 at H；
Figure 19 be the tenth embodiment freezing carrier mechanism in frozen structure structural representation；
Figure 20 is the partial enlarged drawing in Figure 19 at I；
Figure 21 is the structural representation of the freezing carrier unit of an embodiment；
Figure 22 is the sleeve pipe and the structural representation of pipe cap of the freezing carrier unit shown in Figure 21.
Specific embodiment
For the ease of understanding the present invention, the present invention is described more fully below with reference to relevant drawings.In accompanying drawing
Give presently preferred embodiments of the present invention.But, the present invention can be realized in many different forms, however it is not limited to this paper institutes
The embodiment of description.On the contrary, the purpose for providing these embodiments is to make the understanding to the disclosure more thorough
Comprehensively.
It should be noted that when element is referred to as " being installed on " another element, it can directly on another element
Or can also there is element placed in the middle.When an element is considered as " connection " another element, it can be directly connection
To another element or may be simultaneously present centering elements.When an element is considered as " connection " another element, it can
To be directly to another element or may be simultaneously present centering elements.
Unless otherwise defined, all of technology used herein and scientific terminology and the technical field for belonging to the present invention
The implication that technical staff is generally understood that is identical.The term for being used in the description of the invention herein is intended merely to description tool
The purpose of the embodiment of body, it is not intended that of the invention in limiting.Term as used herein "and/or" includes one or more phases
The arbitrary and all of combination of the Listed Items of pass.
As shown in figure 1, a kind of freezing carrier mechanism 100 of the first embodiment of the present invention, including holding structure 110 and freezes
Deposit structure 120.Holding structure 110 is connected with frozen structure 120, and frozen structure 120 includes loading zone and capillarity arrangements
121, loading zone is used for carrying cell, and capillarity arrangements 121 are used for producing capillarity to draw the immersion liquid of loading zone.
The cell for being coated with cryoprotective agent can be transferred to jelly when using by the freezing carrier mechanism 100 of the present embodiment
The loading zone of structure 120 is deposited, and capillarity is produced by capillarity arrangements 121, so as to be coated on pericellular big portion
Divide cryoprotective agent to siphon away, only remain minimal amount of cryoprotective agent liquid film and be coated on cell peripheral.The suction process is by freezing load
Body mechanism 100 is automatic and spontaneous to be completed, and does not rely on manually-operated control, it is achieved that the automatic minimum of cryoprotective agent covering amount
Changing, it is to avoid the difference that technical merit difference is caused, so as to can reach the purpose of operational standardization control, and then effectively carrying
The survival rate of high ovum/zygote/embryo and anabiosis rate, it is ensured that its potentiality of development.
In the present embodiment, freezing carrier mechanism 100 also includes falling 114 again, again pendant 114 located at holding structure 110 away from
One end of frozen structure 120, for increasing the weight of freezing carrier 100, improves stability.Specifically, holding structure 110 includes
For holding the shank body 111 of operation, 111 one end of shank body is provided with the frozen structural connection 112 being connected with frozen structure 120, handle
111 other end of body is provided with pendant fixed part 113 again is used for fixed pendant 114 again.In the present embodiment, holding structure 110 and frozen knot
Structure 120 is integrally formed, the freezing carrier mechanism 200 of second embodiment that also can be as shown in Figure 2, is formed by connecting by each several part.
Preferably, frozen structure 120 improves wellability using TC (Tissue culturetreated) process, strengthens hair
Thin action effect.Holding structure 110 is made up of materials such as low temperature resistant macromolecular material, metal, glass or carbon fibers.Frozen structure
120 are made up of materials such as transparent low temperature resistant macromolecular material, glass, metals.
As shown in Figure 3 and Figure 4, in the first embodiment, capillarity arrangements 121 are provided with for producing capillary opening
Mouth 1211 or gap, loading zone are located at the region that capillarity arrangements 121 are provided with opening 1211 or gap, and be open 1211 or seam
The diameter for being smaller in size than cell to be carried of gap.Thus, when glass freezing is operated, by cell and the cold of cell can be coated
Freeze protective agent be transferred to positioned at be provided with opening 1211 or gap region loading zone, cryoprotective agent touch opening 1211 or
Siphoned away by capillarity major part automatically behind gap, but cell cannot be siphoned away, realized cryoprotective agent covering amount oneself
Dynamic minimum.
Preferably, capillarity arrangements 121 be tubular structure, end face of the capillarity arrangements 121 away from holding structure 110
The inclined-plane being obliquely installed is provided with, opening 1211 is located at the inclined-plane.Specifically, the size of capillarity arrangements 121 is to away from fixing
110 one end of structure is gradually reduced, and the axis of inclined-plane and capillarity arrangements 121 is at an acute angle such as 10 degree, 20 degree etc., capillarity knot
External diameter is 300 μm at one end for 1211 institute of opening of structure 121, and the diameter of opening 1211 is less than the diameter such as 50 of ovum/zygote/embryo
μm, the other end external diameter away from opening 1211 is 2.0mm, and the amount of liquid that capillarity arrangements 121 can receive is more than 1 μ L.At this
In embodiment, the 1211 equal ovalizes of inclined-plane and opening of opening 1211 are offered.It is appreciated that capillarity arrangements 121
Size and dimension not limited to this, cross section can be circle, and the capillary in 3rd embodiment that also can be as shown in Figure 5 and Figure 6 is made
With structure 121, the inclined-plane for being provided with opening is rectangular with opening 1211.
The freezing carrier mechanism 100 of the present embodiment produces capillarity by frozen structure 120, so as to be coated on cell
Most of cryoprotective agent of surrounding is siphoned away, and is only remained minimal amount of cryoprotective agent liquid film and is coated on cell peripheral, and this was drawn
Journey is completed by freezing carrier mechanism 100 is automatic and spontaneous, does not rely on manually-operated control, it is achieved that cryoprotective agent covering amount
Automatic minimum, can reach the purpose of operational standardization control, and then effectively improve the survival of ovum/zygote/embryo
Rate and anabiosis rate, it is ensured that its potentiality of development.
As shown in Figure 7 and Figure 8, the freezing carrier mechanism of the fourth embodiment that the present invention is provided includes and first embodiment
110 identical holding structure (not shown) of holding structure in freezing carrier mechanism 100, difference is, in the present embodiment
In, the capillarity arrangements 421 of tubular structure at least have two relative sides, and at least one of capillarity arrangements 421
Side offers dead slot 4213, so that the gap between two relative sides is formed for producing capillary gap
4212, the diameter for being smaller in size than cell to be carried in gap 4212, loading zone are located at capillarity arrangements 421 and form gap
4212 region.Thus, when glass freezing is operated, the cryoprotective agent of cell and cladding cell can be transferred to loading
Area, cryoprotective agent are siphoned away by capillarity major part after touching gap 4212 automatically, but cannot siphon away cell, real
The automatic minimum of existing cryoprotective agent covering amount.
It is appreciated that the shape of dead slot 4213 can be arranged as required to, the dead slot 4213 in the present embodiment is c-type or circle
Shape, can only be located at a side, can also be located at multiple sides, also can be located at the correspondence position of two relative sides so as to being formed
Through hole.Specifically in the present embodiment, the rounded rectangle in the cross section of capillarity arrangements 421, and the width of the rectangle is less than institute
The diameter of cell to be carried.Circular dead slot can be opened in wherein one or two long side place side of capillarity arrangements 421
Middle part rounded, C-shaped dead slot can be opened in the edge of one or two long side place side.
As shown in Figure 9 and Figure 10, the freezing carrier mechanism of the 5th embodiment that the present invention is provided includes and first embodiment
Freezing carrier mechanism 100 in 110 identical holding structure (not shown) of holding structure, difference is, in this enforcement
In example, the capillarity arrangements 521 of tubulose include the extension 5213 for being connected to holding structure and to be connected to extension 5213 remote
From the bending section 5214 of holding structure one end, 5214 bending extension of bending section, bending section 5214 are not connected with the end of extension 5213
Face is offered for producing capillary opening 5211, and with 5213 interval setting of extension and spacing distance is less than will hold
The diameter of cell is carried, the diameter for being smaller in size than cell to be carried of opening 5211, loading zone are set positioned at capillarity arrangements 521
There is the region of opening 5211.
Thus, when glass freezing is operated, the cryoprotective agent of cell and cladding cell can be transferred to bending section
5214 loading zone, cryoprotective agent are siphoned away by capillarity major part after touching opening 5211 automatically, are realized cold
Freeze the automatic minimum of protective agent covering amount, and due to 5211 place end face of opening and capillarity arrangements 521 lateral wall away from
From the diameter less than cell to be carried, can avoid being blocked by cell in the protectant process split shed 5211 of absorption freezing
Wicking process is caused to terminate in advance.
It is appreciated that the form and size of bending section 5214 can be arranged as required to, and in the present embodiment, bending section 5214
Ring-type in ρ shapes, length 10mm, overall diameter are close to such as 120 μm of the external diameter of ovum/zygote/embryo, and the bore of opening 5211 is less than
Such as 50 μm of the external diameter of ovum/zygote/embryo, the 5211 place end faces that are open are outer with the extension 5213 of capillarity arrangements 521
Sidewall spacers distance is less than 50 μm.
As is illustrated by figs. 11 and 12, the freezing carrier mechanism of the sixth embodiment that the present invention is provided includes and first embodiment
Freezing carrier mechanism 100 in 110 identical holding structure (not shown) of holding structure, difference is, in this enforcement
In example, capillarity arrangements 621 include that cellosilk 6213 and connecting tube 6214, at least one end of cellosilk 6213 stretch into connecting tube
In 6214, so as to be formed between the tube wall of cellosilk 6213 and connecting tube 6214 for producing capillary gap 6212, stitch
The diameter for being smaller in size than cell to be carried of gap 6212, loading zone are located at the area that capillarity arrangements 621 form gap 6212
Domain.
Thus, when glass freezing is operated, can first by the end cryoprotection of cellosilk 6213 and connecting tube 6214
Agent infiltrates, so that 6213 surface of cellosilk covers liquid film, the gap between 6214 tube wall of cellosilk 6213 and connecting tube
6212 have sucked a little cryoprotective agent, can strengthen capillary action effect, then by cell and the cryoprotection of cladding cell
Agent is transferred to the loading zone of cellosilk 6213, cryoprotective agent touch behind gap 6212 i.e. by capillarity most of by from
Move and siphon away, realize the automatic minimum of cryoprotective agent covering amount.
In the present embodiment, connecting tube 6214 is wholely set with holding structure, and the internal diameter of connecting tube 6214 is 150 μm, fine
The external diameter of dimension silk 6213 is 100 μm, and it is 2mm that cellosilk 6213 is located at the partial-length outside connecting tube 6214, it will be understood that size
Form not limited to this, can be adjusted as needed.
As shown in Figure 13 and Figure 14, the freezing carrier mechanism of the 7th embodiment that the present invention is provided includes and first embodiment
Freezing carrier mechanism 100 in 110 identical holding structure (not shown) of holding structure, difference is, in this enforcement
In example, capillarity arrangements 721 include that cellosilk 7213 and connecting tube 7214, at least one end of cellosilk 7213 stretch into connecting tube
In 7214, so as to be formed between the tube wall of cellosilk 7213 and connecting tube 7214 for producing capillary gap 7212, stitch
The diameter for being smaller in size than cell to be carried of gap 7212, and the quantity of cellosilk 7213 is at least two, at least two fibers
Silk 7213 is parallel and contacts with each other, and loading zone is located at the region that capillarity arrangements 721 form gap 7212.
Thus, between cellosilk 7213 can drain cryoprotective agent, when glass freezing is operated, can first by cellosilk
7213 and connecting tube 7214 stretch in frozen solution infiltrate so that 7213 surface of cellosilk covers liquid film, cellosilk
Having sucked cryoprotective agent between 7213 makes gap less, the gap 7212 between 7214 tube wall of cellosilk 7213 and connecting tube
A little cryoprotective agent is sucked, capillary action effect is further enhancing.Then by cellosilk 7213 be lifted away from frozen solution or
Person makes the end of cellosilk 7213 keep contacting with frozen solution, then the cryoprotective agent of cell and cladding cell is transferred to
The loading zone of cellosilk 7213, cryoprotective agent are drained to the cold of 7213 one end of connecting tube 7214 or cellosilk by cellosilk 7213
Freeze protection liquid, most of cryoprotective agent is sucked away immediately, realizes the automatic minimum of cryoprotective agent covering amount.
Preferably, at least one cellosilk 7213 is hard state fibers straight, such as hard state rustless steel fibers straight.In the present embodiment
In, the quantity of cellosilk 7213 is 2, and the external diameter of cellosilk 7213 is 40 μm, and the internal diameter of connecting tube 7214 is slightly larger than cellosilk
Such as 100 μm of 7213 external diameter sum.It is appreciated that size modes not limited to this, can be adjusted as needed.
As shown in Figure 15 and Figure 16, the freezing carrier mechanism of the 8th embodiment that the present invention is provided includes and first embodiment
Freezing carrier mechanism 100 in 110 identical holding structure (not shown) of holding structure, difference is, in this enforcement
In example, capillarity arrangements 821 include that cellosilk 8213 and connecting tube 8214, the two ends of cellosilk 8213 stretch into connecting tube
8214, so that cellosilk 8213 is located at the part outside connecting tube 8214 and forms circulus, and cellosilk 8213 and connecting tube
Formed between 8214 tube wall for producing capillary gap 8212, gap 8212 is smaller in size than cell to be carried
Diameter, loading zone are located at the region that capillarity arrangements 821 form gap 8212.
Thus, when glass freezing is operated, first cellosilk 8213 and connecting tube 8214 can be infiltrated with cryoprotective agent,
Then the loading zone that the cryoprotective agent of cell and cladding cell is transferred to circulus is formed drop or liquid film, cryoprotection
Agent is touched gap 8212 and is sucked away by capillarity major part immediately, realizes the automatic minimum of cryoprotective agent covering amount
Change.In the present embodiment, the external diameter of cellosilk 8213 is 50 μm, and the internal diameter of connecting tube 8214 is slightly larger than 8213 external diameter of cellosilk
Such as 110 μm of twice.
As shown in Figure 17 and Figure 18, the freezing carrier mechanism 900 of the 9th embodiment that the present invention is provided includes real with first
The 110 identical holding structure 910 of holding structure in the freezing carrier mechanism 100 of example is applied, difference is, in the present embodiment
In, capillarity arrangements 921 are at least two cellosilks 9213, are formed for producing capillary between at least two cellosilks 9213
The gap 9212 of effect, the diameter for being smaller in size than cell to be carried in gap 9212,9213 one end of cellosilk and holding structure
Connection, loading zone are located at the region that capillarity arrangements 921 form gap 9212.
Thus, when glass freezing is operated, first cellosilk 9213 can be stretched in frozen solution and be infiltrated, then be raised and make
The end of cellosilk 9213 keeps contacting with frozen solution, and the cryoprotective agent of cell and cladding cell is transferred to cellosilk
9213 loading zone, most of cryoprotective agent are then drawn to freezing by the gap 9212 between cellosilk 9213 by capillarity and protect
Shield liquid, realizes the automatic minimum of cryoprotective agent covering amount.
Preferably, at least one cellosilk 9213 is hard state fibers straight, such as hard state rustless steel fibers straight.In the present embodiment
In, the length of cellosilk 9213 is 4mm, and quantity is 2, and external diameter is 40 μm, it will be understood that size modes not limited to this, many
The external diameter of individual cellosilk 9213 also can be inconsistent, is adjusted as needed.
As illustrated in figures 19 and 20, the freezing carrier mechanism of the tenth embodiment that the present invention is provided includes and first embodiment
Freezing carrier mechanism 100 in 110 identical holding structure (not shown) of holding structure, difference is, in this enforcement
Example in, frozen structure 1020 include loading zone, capillarity arrangements 1021 and water suction bar 1022, capillarity arrangements 1021 be to
Few two cellosilks 10213, form between at least two cellosilks 10213 for producing capillary gap 10212, gap
10212 diameter for being smaller in size than cell to be carried, water suction bar 1022 be connecteds with holding structure, cellosilk 10213 and absorb water
Bar 1022 is connected to be connected with holding structure by the bar 1022 that absorbs water, and loading zone is located at capillarity arrangements 1021 and forms gap
10212 region.
Thus, when glass freezing is operated, cellosilk 10213 and part water suction bar 1022 can be used frozen solution first
Infiltration, then be lifted away from frozen solution or make the end of cellosilk 10213 keep contacting with frozen solution, then by cell and
The cryoprotective agent of cladding cell is transferred to the loading zone of cellosilk 10213, and most of cryoprotective agent passes through cellosilk immediately
Gap 10212 between 10213 is drained in the frozen solution of water suction bar 1022 or the other end of one end, and water suction bar 1022 is effective
Enhance capillary action effect, it is achieved that the automatic minimum of cryoprotective agent covering amount.
As shown in figure 21 and figure, a kind of freezing carrier unit 500, including above-mentioned freezing carrier mechanism 100 and sleeve pipe
300, freezing carrier mechanism 100 is contained in sleeve pipe 300 and can be provided with axial limiting along 300 axially-movable of sleeve pipe, sleeve pipe 300
Structure (not shown) is limiting the ultimate range that freezing carrier mechanism 100 moves to 20 place direction of frozen structure.
The freezing carrier unit 500 of the present embodiment, its freezing carrier mechanism 100 are contained in sleeve pipe 300, can be effective
Protection freezing carrier mechanism 100, especially frozen structure 120 is less, collides and is easily deformed or fractures, and therefore can prevent from transporting
Damage during defeated storage.Meanwhile, freezing carrier mechanism 100 along 300 axially-movable of sleeve pipe, and can have axial limit structure to limit
Move distance processed, so that need to only incline certain angle, you can make the frozen structure of freezing carrier mechanism 100 under gravity
120 skid off outside sleeve pipe 300 or return in sleeve pipe 300, without the need for be inserted in freezing carrier mechanism 100 during freeze-thaw manually
Sleeve pipe 300 takes out, and causes freezing carrier mechanism 100 to collide with sleeve pipe 300 and is damaged so as to avoid operational error, make
With more convenient, safety.
Specifically, axial limit structure is the contraction mouth that 300 one end internal wall of pipe orifice of sleeve pipe inwardly reduces formation, or is sleeve pipe
Axially arranged annulus in 300 one end mouths of pipe, can control freezing load by adjusting the external diameter of different parts of holding structure 110
Ultimate range of the body mechanism 100 along 300 axially-movable of sleeve pipe.It is appreciated that the structure not limited to this of axial limit structure, can root
According to needing to arrange, for example, the annulus with through hole is arranged on holding structure 110, being provided with sleeve pipe 300 can be with the annulus mounting
Hook.
In the present embodiment, 300 cylindrical structure of sleeve pipe, including casing main body 301 and is respectively arranged on casing main body 301
The pullover 302 at two ends and set tail 303, near pullover 302, set tail 303 is that open end such as passes through sleeve pipe master to frozen structure 120
301 one end of body reduces to form contraction mouth or filling non-hermetic material, or closed type terminal as by casing main body 301
One end reduces to form cecum or filling sealing material；Maximum of the minimum diameter of axial limit structure more than frozen structure 120
External diameter, footpath difference are 0.5mm；Maximum outside diameter of the minimum diameter of axial limit structure less than holding structure 110, footpath difference are 0.5mm；
Length of the length of freezing carrier mechanism 100 less than 301 inner chamber of casing main body, length difference is 3mm；Freezing carrier mechanism 100
Internal diameter of the maximum outside diameter less than casing main body 301, footpath difference are 0.5mm.It is appreciated that size modes not limited to this, can be according to need
Adjust.
Preferably, freezing carrier unit 500 also includes that pipe cap 400, pipe cap 400 can be sheathed on sleeve pipe 300 near frozen knot
One end of structure 20, so that further strengthen the protection to frozen structure 120.
In the present embodiment, pipe cap 400 is cylindrical, including pipe cap main body 401 and is respectively arranged on 401 two ends of pipe cap main body
Cap head 402 and cap tail 403, cap head 402 radially extends in infundibulate so as to playing the guiding role when sleeve pipe 300 is sheathed on, covers
Pipe 300 is connected with 400 interference of pipe cap；Cap tail 403 is that open end such as reduces to form contraction mouth by 401 one end of pipe cap main body
Or filling non-hermetic material, or closed type terminal are as reduced to form cecum or filling by 401 one end of pipe cap main body
Sealing material；The internal diameter of pipe cap main body 401 is slightly less than the external diameter of sleeve pipe 300, and footpath difference is 0.1mm；The maximum outside diameter of cap head 402
More than the external diameter of pullover 302, footpath difference is 0.2mm.It is appreciated that size modes not limited to this, can be adjusted as needed.
Preferably, the lateral wall of sleeve pipe 300 is provided with pipe cap position limiting structure 310, is sheathed on sleeve pipe 300 to limit pipe cap 400
Depth capacity, so as to prevent colliding with the bottom of pipe cap 400 during 100 axially-movable of freezing carrier.It is appreciated that mode
Not limited to this, for example, can also arrange warning line, or the internal diameter change of control pipe cap 400 in the lateral wall of sleeve pipe 300.At this
In embodiment, freezing carrier mechanism 100 along sleeve pipe 300 axially move to ultimate range to 120 place direction of frozen structure when, freeze
Structure 120 is deposited with the bottom surface of the pipe cap 400 for being sheathed on sleeve pipe 300 apart from 3mm.
The freezing carrier unit 500 of the present embodiment, its freezing carrier mechanism 100 are contained in sleeve pipe 300,400 sets of pipe cap
Located at sleeve pipe 300, be that frozen structure 120 provides effective protection, freezing carrier mechanism 100 can along 300 axially-movable of sleeve pipe, and
There is axial limit structure to limit largest motion distance, so as to need to only incline certain angle, you can to carry freezing under gravity
The frozen structure 120 of body mechanism 100 is skidded off outside sleeve pipe 300 or is returned in sleeve pipe 300, without the need for manually will during freeze-thaw
Freezing carrier mechanism 100 is inserted in sleeve pipe 300 or takes out, and causes freezing carrier mechanism 100 and sleeve pipe so as to avoid operational error
300 collide and are damaged, using more convenient, safety.
Each technical characteristic of embodiment described above arbitrarily can be combined, for making description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more concrete and detailed, but simultaneously
Therefore can not be construed as limiting the scope of the patent.It should be pointed out that for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, some deformations and improvement can also be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be defined by claims.
Claims (10)
1. a kind of freezing carrier mechanism, for carrying cell, it is characterised in that the freezing carrier mechanism include holding structure and
The frozen structure being connected with the holding structure, the frozen structure include loading zone and capillarity arrangements, the loading zone
For carrying the cell, the capillarity arrangements are used for producing capillarity to draw the immersion liquid of the loading zone.
2. freezing carrier mechanism according to claim 1, it is characterised in that the capillarity arrangements are provided with for producing
Capillary opening or gap, the loading zone are located at the area that the capillarity arrangements are provided with the opening or the gap
The diameter for being smaller in size than the cell in domain, the opening or gap.
3. freezing carrier mechanism according to claim 2, it is characterised in that the capillarity arrangements are tubular structure.
4. freezing carrier mechanism according to claim 3, it is characterised in that the capillarity arrangements at least have two
Relative side, at least one side of the capillarity arrangements offer dead slot so that described two relative sides it
Between gap form the gap.
5. freezing carrier mechanism according to claim 3, it is characterised in that the capillarity arrangements include being connected to institute
State the extension of holding structure and be connected to bending section of the extension away from described holding structure one end, the bending section is curved
Qu Yanshen, the bending section are not connected with the end face of the extension and are provided with the opening, and with the extension interval setting and
Diameter of the spacing distance less than the cell.
6. freezing carrier mechanism according to claim 2, it is characterised in that the capillarity arrangements include cellosilk and
Connecting tube, described filametntary at least one end are stretched in the connecting tube, so that the tube wall of the cellosilk and the connecting tube
Between form the gap.
7. freezing carrier mechanism according to claim 6, it is characterised in that the filametntary quantity is at least two,
Cellosilk described at least two is parallel and contacts with each other.
8. freezing carrier mechanism according to claim 2, it is characterised in that the capillarity arrangements are at least two fine
Dimension silk, forms the gap between cellosilk described at least two, described cellosilk one end is connected with the holding structure.
9. freezing carrier mechanism according to claim 8, it is characterised in that the frozen structure also includes the bar that absorbs water, institute
State water suction bar to be connected with the holding structure, the cellosilk is connected with the water suction bar with solid with described by the water suction bar
Hold structure connection.
10. a kind of freezing carrier unit, it is characterised in that include the freezing carrier mechanism as described in any one of claim 1-9
And sleeve pipe, the freezing carrier mechanism is contained in described sleeve pipe and can be provided with axle along described sleeve pipe axially-movable, described sleeve pipe
To position limiting structure limiting the ultimate range that the freezing carrier mechanism moves to the frozen structure place direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611236911.XA CN106489915A (en) | 2016-12-28 | 2016-12-28 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611236911.XA CN106489915A (en) | 2016-12-28 | 2016-12-28 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
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| CN106489915A true CN106489915A (en) | 2017-03-15 |
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| CN201611236911.XA Pending CN106489915A (en) | 2016-12-28 | 2016-12-28 | Freezing carrier mechanism and the freezing carrier unit including the freezing carrier mechanism |
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| CN110476952A (en) * | 2019-09-06 | 2019-11-22 | 苏州贝康医疗器械有限公司 | Vitrified frozen vector |
| EP4056035A4 (en) * | 2019-12-24 | 2023-12-27 | Shanghai Horizon Medical Science Co., Ltd. | SUPPORT, VACUUMATION DEVICE AND SYSTEM FOR TISSUE CRYOPRESERVATION |
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| CN110476952A (en) * | 2019-09-06 | 2019-11-22 | 苏州贝康医疗器械有限公司 | Vitrified frozen vector |
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Application publication date: 20170315 |