CN106442419B - Self compensation SPR optical fiber biochemical sensor - Google Patents
Self compensation SPR optical fiber biochemical sensor Download PDFInfo
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- CN106442419B CN106442419B CN201610806716.XA CN201610806716A CN106442419B CN 106442419 B CN106442419 B CN 106442419B CN 201610806716 A CN201610806716 A CN 201610806716A CN 106442419 B CN106442419 B CN 106442419B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/031—Multipass arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
- G01N21/554—Attenuated total reflection and using surface plasmons detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/127—Calibration; base line adjustment; drift compensation
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
一种自补偿SPR光纤生化传感器，属于光纤生化传感技术领域。将微型石英毛细管作为传感元件，在其表面相邻部位分别镀制金膜及固定单层的金纳米粒子，并在其内部注入去离子水，形成LSPR体折射率补偿通道及FP温度补偿通道。卤钨灯提供宽带光源，利用多模光纤进行光的传输，输出信号通过光谱仪传入电脑，通过电脑软件对蛋白质样品的特异性结合过程进行实时的数据采集、记录和处理。本发明利用单一的传感元件即可同时获得测试信号和两种补偿信号，无需附加的补偿通道即可实现高精度检测。本发明无需抛光研磨，激光加工，化学腐蚀，生化膜系合成等手段，制作工艺简单，价格低廉，实现了高精度、高灵敏度，方便快捷的生化样品检测分析。
A self-compensating SPR optical fiber biochemical sensor belongs to the technical field of optical fiber biochemical sensing. The micro-quartz capillary is used as the sensing element, and the adjacent parts of the surface are coated with gold film and fixed single-layer gold nanoparticles, and deionized water is injected into it to form the LSPR bulk refractive index compensation channel and FP temperature compensation channel . The tungsten halogen lamp provides a broadband light source, and uses multimode optical fiber for light transmission. The output signal is transmitted to the computer through the spectrometer, and the specific binding process of the protein sample is collected, recorded and processed in real time through the computer software. The present invention can obtain the test signal and two compensation signals simultaneously by using a single sensing element, and can realize high-precision detection without an additional compensation channel. The method does not need polishing and grinding, laser processing, chemical corrosion, biochemical film synthesis and other means, has simple manufacturing process and low price, and realizes high precision, high sensitivity, and convenient and quick detection and analysis of biochemical samples.
Description
Technical field
The invention belongs to optical-fiber bio field of sensing technologies, obtain three on single self compensation SPR optical fiber biochemical sensor
Kind different detections and thermal compensation signal, be related to surface plasma body resonant vibration (SPR), local surface plasma resonance (LSPR) and
Fabry-Perot (FP) sensing principle.Whole system structure is simple, high sensitivity, can be to ambient temperature and body refractive index
Self compensation is carried out, and can be used for the real-time quick detection of biological sample, is had in physics, biology, chemistry and medical domain extensive
Prospect.
Background technique
Surface plasma body resonant vibration (Surface Plasmon Resonance, SPR) is a kind of physical optics phenomenon, when
The optical fiber surface that light beam injection is coated with certain thickness metallic film is totally reflected, and when meeting certain condition, light is coupled
Into metallic film, electronics is caused to resonate, i.e. generation SPR effect.Since SPR phenomenon is to the medium refraction index of metal surface
It is very sensitive, related transducer related with refractive index can be carried out.Compared with traditional sensors, optical fiber SPR sensor has miniature
The advantages that change, high sensitivity, electromagnetism interference, fast response time.Since SPR effect has very high sensitivity, to extraneous ring
The variation in border is very sensitive, therefore more demanding to sensed condition, and most optical fiber SPR sensors are applied to scientific research field more.Cause
This needs designs the highly sensitive, optical fiber SPR sensor with self-compensating function, the self compensation SPR sensorgram reported at present
Device is mostly single compensation channel, therefore compensation becomes the urgent of sensory field of optic fibre and is essential while can be realized temperature and refractive index
It asks.
Summary of the invention
The present invention is intended to provide a kind of highly sensitive, high-precision self compensation SPR optical fiber biochemical sensor.The sensor base
In SPR, local surface plasma resonance (Localized Surface Plasmon Resonance, LSPR), Fabry wave
Sieve (Febry-Perot, FP) sensing principle obtains three kinds of detection letters simultaneously in the simple miniature quartz capillary pipe structure of structure
Number.Due to compensation while the invention realizes ambient temperature and body refractive index, which wants working environment
It asks extremely low, realizes highly sensitive, high-precision, the biochemistry detection of low cost, there is wide application prospect in biochemistry detection field.
Technical solution of the present invention:
Self compensation SPR optical fiber biochemical sensor, including gold nanoparticle 11, golden film 12, miniature quartz capillary 13, go from
Sub- water filling liquid 14, miniature quartz capillary 13 and it is interior be marked with deionized water filling liquid 14, miniature quartz capillary outer wall is by gold
11 layers of film 12 and single gold nanoparticle constitute two channels continuously or discontinuously, and SPR/LSPR/FP triple channel structure is integrally formed,
As self compensation SPR optical fiber biochemical sensor；In self compensation SPR optical fiber biochemical sensor both ends constant transmissions optical fiber, it is placed in stream
In logical pond, both ends are fixed；Flow cell is equipped with sample inflow entrance and sample outflux.
The invention has the advantages that: the present invention realizes three using simple self compensation SPR optical fiber biochemical sensor
Channel measurement can compensate the error that ambient temperature and body refractive index generate SPR detection signal simultaneously.Without additional
Compensation channel can be realized high-precision and detect.Furthermore the invention is not necessarily to polishing grinding, laser processing, chemical attack, biochemical membrane system
The means such as synthesis, manufacture craft is simple, cheap, realizes high-precision, highly sensitive biological sample tests and analyzes.In life
Object medical treatment detection field has good prospect.
Detailed description of the invention
Fig. 1 is experimental system schematic diagram.
Fig. 2 is the schematic diagram of self compensation SPR optical fiber biochemical sensor.
Fig. 3 is position flow cell schematic diagram.
In figure: 1 wideband light source；2 transmission fibers；3 self compensation SPR optical fiber biochemical sensors；4 water-baths；5 fiber spectrums
Instrument；
6 computers；7 samples to be tested；8 peristaltic pumps；9 sample flow hoses；10 waste liquid pools；
11 gold nanoparticles；12 golden films；13 miniature quartz capillaries；14 deionized water filling liquids；
15 flow cells；16 sample inflow entrances；17 sample outfluxes.
Specific embodiment
A specific embodiment of the invention is described in detail below in conjunction with technical solution and attached drawing.
1. preparation process
1) micro-structure processing is carried out using grinding technique to the end face of multimode transmissions optical fiber and miniature quartz capillary, by end
Face is ground into plane；
2) gold nanoparticle is attached to miniature quartz capillary outer wall using chemical treatment；
3) golden film of 50nm is deposited in miniature quartz capillary outer wall using magnetron sputtering technique；
4) deionized water is imported in quartz capillary using siphonage；
5) one end of self compensation SPR optical fiber biochemical sensor is connected on light source with multimode transmissions optical fiber, the other end connects
It connects on fiber spectrometer, and fiber spectrometer is connected with computer.
2. operation
Test device is as shown in Figure 1.
1) by transmission fiber SPR effect occurs at golden film for the light issued by wideband light source, adheres to single layer of gold nanoparticle
LSPR effect occurs for the surface of son, and FP interference occurs in the capillary of filling deionized water, SPR/LSPR/FP effect occurs
Light is received by fiber spectrometer and is transferred to the acquisition and processing that computer carries out signal.
2) self compensation SPR optical fiber biochemical sensor is encapsulated in self-control flow cell, the biography of sample is carried out using peristaltic pump
It is defeated, it is possible to reduce the volatilization of sample and to be conveniently replaceable.
3) when sample to be tested does not flow into flow cell, sensing unit dry tack free is kept, light source is successively lighted and extinguish,
It is composed by spectrometer collection background spectrum and half-light；
4) calibration solution of different refractivity is slowly introducing flow cell using peristaltic pump, using spectrometer to corresponding spectrum
Figure is acquired, and obtains normalization light spectrogram, and SPR test signal and LSPR compensation of refractive index signal refractive index have good sound
It answers, and FP temperature compensation signal refractive index is insensitive；
5) gradient-heated is carried out to self compensation SPR optical fiber biochemical sensor using water-bath, using spectrometer to corresponding
Spectrogram is acquired, and obtains normalization light spectrogram, and SPR test signal and FP temperature compensation signal have good sound to temperature
It answers, and LSPR compensation of refractive index signal is to Temperature Insensitive；
6) the sensing unit surface of self compensation SPR optical fiber biochemical sensor is chemically modified, protein can be realized
Specifically bind identification function.
Claims (1)
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| CN201610806716.XA CN106442419B (en) | 2016-09-06 | 2016-09-06 | Self compensation SPR optical fiber biochemical sensor |
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| CN114235754A (en) * | 2021-12-18 | 2022-03-25 | 桂林电子科技大学 | Optical fiber SPR sensor with temperature compensation based on capillary tube |
| CN115575355A (en) * | 2022-10-12 | 2023-01-06 | 大连理工大学 | Multichannel prism surface plasmon resonance sensor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US6480282B1 (en) * | 1999-05-06 | 2002-11-12 | University Of Washington | Capillary surface plasmon resonance sensors and multisensors |
| US7352468B2 (en) * | 2001-12-12 | 2008-04-01 | Trustees Of Princeton University | Cavity ring-down detection of surface plasmon resonance in an optical fiber resonator |
| CN100458406C (en) * | 2005-07-01 | 2009-02-04 | 重庆工学院 | MZ interference SPR chemical and biological sensor and system with fibre-optical microstructure |
| TWI404982B (en) * | 2009-09-22 | 2013-08-11 | Nat Univ Chung Cheng | Localized plasma resonance sensing device |
| CN102507503B (en) * | 2011-10-24 | 2013-11-27 | 天津大学 | Fiber-optic surface plasmon resonance glucose sensor with temperature self-compensation |
| US9354179B2 (en) * | 2012-06-10 | 2016-05-31 | Bio-Rad Laboratories Inc. | Optical detection system for liquid samples |
| CN105866070A (en) * | 2016-05-27 | 2016-08-17 | 哈尔滨工程大学 | Distributed liquid refractive index sensing device based on optical fiber surface plasma resonance |
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