CN106405128B - A kind of medical treatment detection sample immunoassay system - Google Patents
A kind of medical treatment detection sample immunoassay system Download PDFInfo
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- CN106405128B CN106405128B CN201610735529.7A CN201610735529A CN106405128B CN 106405128 B CN106405128 B CN 106405128B CN 201610735529 A CN201610735529 A CN 201610735529A CN 106405128 B CN106405128 B CN 106405128B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
A kind of medical treatment detection sample analysis system, including：Storage part, for storing the related coefficient between the single measurement range of different detection projects and each detection project；Division is divided into first portion's sample and second portion sample for that will detect sample；Dilution part, for first portion's sample to be diluted according to specified multiple；Mating portion for the data according to project to be detected and storage part, determines the pairing detection project with project to be detected pairing；Test section, for carrying out project to be detected and the detection for matching detection project for first portion's sample after dilution and the detection of pairing detection project being carried out for second portion sample；Calculating part according to the testing result of first portion's sample and second portion sample, determines concentration before the dilution of project to be detected.
Description
Technical field
The present invention relates to a kind of sample analysis system, more particularly, to a kind of medical treatment detection sample automated immunochemistry analysis system
System.
Background technology
Immunoassay is a kind of special reagent analysis method of form, the detection side carried out using antigen-antibody reaction
Method, i.e., using antigen and the specific reaction of antibody, using the antigen or antibody prepared as reagent, to detect in sample
Corresponding antibodies or antigen.Because it is with high specific, it is highly sensitive the characteristics of, be widely used to preclinical medicine and clinical examination
The fields of grade, and its application range gradually expands
In the prior art, in immunoassay apparatus and other sample analyzers, when the measured ingredient in sample
Cause excessive concentration for the positive, in the concentration range for usually being covered Sample Dilution to calibration curve, then will sample after dilution
Measured value for calibration curve, the concentration of measured ingredient contained in the sample is obtained.However, the dilution of sample is surveyed
Surely it may cause there are error, influence the accuracy of testing result.
To solve the above-mentioned problems, SYSMEX Co., Ltd proposes a kind of sample analysis in 201310015352X
Method and apparatus are diluted using the standard sample of known concentration, are obtained and are diluted later detectable concentration, so that it is determined that inspection
The standard curve between concentration and dilute sample is surveyed, actual concentrations are determined by standard curve, are led so as to avoid Sample Dilution
The error of cause.But, it is necessary to predefine standard curve in this method, step is complicated and troublesome.
The content of the invention
The present invention provides a kind of medical treatment detection sample analysis systems, can need not determined in advance using standard sample
In the case of standard curve, error caused by sample dilution can be also avoided.
As one aspect of the present invention, a kind of medical treatment detection sample analysis system is provided, including：Storage part is used for
Store different detection projects single measurement range and each detection project between related coefficient；Division, for that will examine
Test specimens are divided into first portion's sample and second portion sample, and the volume of first portion's sample is less than second portion sample
The half of volume；Dilution part, for first portion's sample to be diluted according to specified multiple；Mating portion is treated for basis
The data of detection project and storage part determine the pairing detection project with project to be detected pairing；Test section, for for dilute
First portion sample after releasing carry out project to be detected and the detection for matching detection project and for second portion sample into
The detection of row pairing detection project；Calculating part according to the testing result of first portion's sample and second portion sample, determines
Concentration before the dilution of project to be detected.
Preferably, the single measurement range of the different detection projects is that the detection project can be measured that without dilution
Maximum and minimum value；Related coefficient foundation between each detection project is identified below：For two detections
Mesh A and B are counted according to its in known disease for positive number simultaneously, count results be two detection project A and
The related coefficient of B.
Preferably, the mating portion matches detection project according to following manner：It is definite to be with project related coefficient to be detected
Zero detection project set；For the maximum Cmax and minimum of the detection project single measurement range in detection project set
The ratio C max/Cmin of value Cmin is ranked up, and selects the detection project of Cmax/Cmin values maximum as pairing detection project.
Optionally, the mating portion matches detection project according to following manner：It is definite to be with project related coefficient to be detected
Zero detection project set；For the minimum value Ymin and single in the negative range of the detection project in detection project set
The ratio Ymin/Cmin of the minimum value Cmin of measurement range is ranked up, select the detection project of Ymin/Cmin values maximum as
Match detection project.
Optionally, the mating portion matches detection project according to following manner：It is definite to be with project related coefficient to be detected
Zero detection project set；For the minimum value Ymin and single in the negative range of the detection project in detection project set
The ratio Ymin/Cmin of the minimum value Cmin of measurement range is ranked up, and selects Ymin/Cmin values maximum and more than dilution times
Several detection projects is as pairing detection project；If the maximum of Ymin/Cmin is all no more than dilution in all detection projects
Multiple, select detection project set in the detection project negative range in the minimum of maximum Ymax and single measurement range
The ratio Ymax/Cmin of value Cmin is more than all detection projects of extension rate as pairing detection project collection.Preferably, it is described
Test section arranges successively according to the value of Ymax/Cmin for matching detection project collection from big to small firstly for second portion sample
Detection project detection, until the minimum of actual measured results N2 and the detection project single measurement range of particular detection project
The ratio of value Cmin is more than extension rate, determines the particular detection project for pairing detection project；Then the test section for
First portion's sample after dilution carries out project to be detected and the detection of the particular detection project.
Preferably, the calculating part determines concentration Nb before the dilution of project to be detected according to following manner：Nb=Na × N2/
N1, wherein Na are the project concentration to be detected of first portion's sample after dilution, and N2 is the pairing detection of second portion sample
Purpose testing result, N1 are the testing result of the pairing detection project of first portion's sample after dilution.
Description of the drawings
Fig. 1 is the system construction drawing of the medical treatment detection sample analysis system of the embodiment of the present invention.
Specific embodiment
In order to illustrate more clearly of technical scheme, embodiment will be used simply to be situated between the present invention below
It continues, it should be apparent that, in describing below is only one embodiment of the present of invention, is come for those of ordinary skill in the art
It says, without having to pay creative labor, other technical solutions can also be obtained according to these embodiments, are fallen within
Disclosure of the invention scope.
The medical treatment detection sample analysis system 100 of the embodiment of the present invention, referring to Fig. 1, including storage part 10, division 20,
Dilution part 30, mating portion 40, test section 50 and calculating part 60.
The detection sample of medical treatment detection sample analysis system 100 can be the body fluid such as blood, urine, saliva.Detection project
Can be tumor markers PIC（Plasmin inhibitor complex, plasmin inhibitor compound）、CEA
（Carcinoembryonic antigen, carcinomebryonic antigen）、 AFP（Alpha fetoprotein, alpha-fetoprotein）, infectious disease mark
Will object HBsAg（Hepatitis B virus antigen inspection）、HBsAb（Hepatitis B virus antibody inspection）Etc. well known in the prior art
Measure project.Storage part 10 be used for store different detection projects single measurement range and each detection project between phase
Relation number.Wherein, the single measurement range with detection project is the maximum that can be measured that without the detection project is diluted
And minimum value.Related coefficient foundation between each detection project is identified below：For two detection projects A and B, according to
Know itself in disease while counted for positive number, count results are the related coefficient of two the detection projects A and B.
Division 20 is divided into first portion's sample and second portion sample, wherein first portion for that will detect sample
Sample is for diluting, and the volume of first portion's sample is less than second portion volume of sample, it is preferred that the volume of first portion's sample
Less than the 1/2 of second portion volume of sample, it is furthermore preferred that the volume of first portion's sample is less than the 1/ of second portion volume of sample
3.Dilution part 30 for first portion's sample to be diluted according to specified multiple, can use well known in the prior art
Dilution is used to dilute first portion's sample.
Mating portion 40 for the data according to project to be detected and storage part, determines to match somebody with somebody with what project to be detected was matched
To detection project.Mating portion 40 can use following manner mating portion to match detection project：It determines and project phase relation to be detected
The detection project set that number is zero；Maximum Cmax for the detection project single measurement range in detection project set and
The ratio C max/Cmin of minimum value Cmin is ranked up, and selects the detection project of Cmax/Cmin values maximum as pairing detection
Mesh.
Test section 50, it can be used to sample is detected.Specifically, test section 50 is for the first portion after dilution
Sample carries out project to be detected and the detection for matching detection project and carries out pairing detection project for second portion sample
Detection.
Calculating part 60, the first portion's sample and the testing result of second portion sample provided according to test section 50,
Determine concentration before the dilution of project to be detected.Specifically, before calculating part 60 determines the dilution of project to be detected according to following manner
Concentration Nb：Nb=Na × N2/N1, wherein Na are the project concentration to be detected of first portion's sample after dilution, and N2 is second
Divide the testing result of the pairing detection project of sample, N1 is the detection knot of the pairing detection project of first portion's sample after dilution
Fruit.
By the above embodiment of the present invention medical treatment detection sample analysis system, due to detection Sample Dilution effect for
Project to be detected and the effect for matching detection project are the same, since the detection range of pairing detection project is wider,
Testing result before it is diluted also is able to detect that can eliminate Sample Dilution effect by the ratio calculating of calculating part 60 causes
Error, so as to accurately determine project to be detected testing result.
Due to the actually detected value of pairing detection project and the maximum Cmax of its single measurement range, above-described embodiment
In be likely to occur pairing detection project after Sample Dilution for pairing detection project, the detected value of sample is less than after dilution
The minimum value Cmin of its single measurement range leads to not determine the detected value for matching sample after detection project dilutes.To understand
The certainly problem, in the preferred embodiment of the invention, mating portion 40 can match detection project according to following manner：Determine with it is to be checked
The detection project set that survey project related coefficient is zero；For in the negative range of the detection project in detection project set most
The ratio Ymin/Cmin of the minimum value Cmin of small value Ymin and single measurement range is ranked up, and selects Ymin/Cmin values big
Pairing detection project is used as in the detection project of extension rate.
By above-mentioned preferred embodiment, the concentration that can ensure to match detection project in the sample after dilution is more than its amount
Journey minimum value, so as to ensure the realization of measurement.But when extension rate is excessive, in fact it could happen that Ymin/ in all detection projects
Cmin values are both less than the situation of extension rate.In further preferred embodiment, mating portion 40 is matched according to following manner and detected
Project：Determine the detection project set for being zero with project related coefficient to be detected；For the detection project in detection project set
Negative range in minimum value Ymin and the ratio Ymin/Cmin of minimum value Cmin of single measurement range be ranked up,
It selects Ymin/Cmin values maximum and the detection project more than extension rate is as pairing detection project；If all detections
The maximum of Ymin/Cmin is all not more than extension rate in mesh, selects the negative range of the detection project in detection project set
The ratio Ymax/Cmin of the minimum value Cmin of interior maximum Ymax and single measurement range is more than all detections of extension rate
Project is as pairing detection project collection.Test section 50 when detecting, firstly for second portion sample according to the value of Ymax/Cmin
It arranges and is detected successively for matching the detection project of detection project collection from big to small, until the actual measurement knot of particular detection project
The ratio of the fruit N2 and minimum value Cmin of the detection project single measurement range is more than extension rate, determines the particular detection project
To match detection project；Then the test section carries out project to be detected for first portion's sample after dilution and this is specific
The detection of detection project.By the further preferred embodiment, if can Ymin/Cmin be most in all detection projects
Big value can be also detected all no more than extension rate.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.It is all
Any modifications, equivalent replacements and improvements are made within the spirit and principles in the present invention, are all contained in protection scope of the present invention
It is interior.
Claims (2)
1. a kind of medical treatment detection sample analysis system, including：Storage part, for storing the single measurement model of different detection projects
Enclose and each detection project between related coefficient；The single measurement range of the difference detection project is without dilution
The maximum and minimum value that the detection project can be measured that；Related coefficient between each detection project is according to as follows
It determines：For two detection projects A and B, counted simultaneously for positive number according to its in known disease, count results are
For the related coefficient of two the detection projects A and B；Division is divided into first portion's sample and second for that will detect sample
Aliquot, the volume of first portion's sample are less than the half of second portion volume of sample；Dilution part, for by first
Sample is divided to be diluted according to specified multiple；Mating portion, for the data according to project to be detected and storage part, determine with
The pairing detection project of project pairing to be detected；The mating portion matches detection project according to following manner：Determine with it is to be detected
The detection project set that project related coefficient is zero；For the maximum of the detection project single measurement range in detection project set
The ratio C max/Cmin of value Cmax and minimum value Cmin is ranked up, select the detection project of Cmax/Cmin values maximum as
Match detection project；Test section, for carrying out project to be detected and pairing detection for first portion's sample after dilution
Purpose detects and is carried out for second portion sample the detection of pairing detection project；Calculating part, according to first portion's sample
And the testing result of second portion sample, determine concentration before the dilution of project to be detected.
2. medical treatment detection sample analysis system according to claim 1, it is characterised in that：The calculating part is according to such as lower section
Formula determines concentration Nb before the dilution of project to be detected：Nb=Na × N2/N1, wherein Na are treating for first portion's sample after dilution
Detection project concentration, N2 are the testing result of the pairing detection project of second portion sample, and N1 is first portion's examination after dilution
The testing result of the pairing detection project of sample.
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| CN201610735529.7A CN106405128B (en) | 2016-08-27 | 2016-08-27 | A kind of medical treatment detection sample immunoassay system |
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| CN201610735529.7A CN106405128B (en) | 2016-08-27 | 2016-08-27 | A kind of medical treatment detection sample immunoassay system |
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| CN106405128B true CN106405128B (en) | 2018-05-25 |
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| JP3206999B2 (en) * | 1992-12-18 | 2001-09-10 | オリンパス光学工業株式会社 | Method for detecting sample dilution error and apparatus for detecting sample dilution error using the same |
| EP0866134A3 (en) * | 1997-03-17 | 1999-12-22 | Hitachi, Ltd. | A calibrator for measuring enzyme activity, and a method for measuring enzyme activity using the same |
| JP5924950B2 (en) * | 2012-01-20 | 2016-05-25 | シスメックス株式会社 | Sample analyzer |
| JP5932540B2 (en) * | 2012-07-24 | 2016-06-08 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| CN103048310B (en) * | 2012-12-31 | 2015-07-22 | 攀钢集团攀枝花钢铁研究院有限公司 | Method for detecting content of impurity elements in electrolyte of vanadium cell |
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Inventor after: Li Fuhu Inventor before: Wang Wei |
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Effective date of registration: 20180428 Address after: 200433 Huang Xing Road, Yangpu District, Shanghai, room 901, room 1675 Applicant after: Shanghai Chen Xiang Medical Equipment Co., Ltd. Address before: 100039 Beijing Fengtai District Sanhuan new town 8 hospital 18 building 1730 Applicant before: Wang Wei |
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Effective date of registration: 20211105 Address after: Room 902, No. 1675, Huangxing Road, Yangpu District, Shanghai 200082 Patentee after: Shanghai Qijia Biotechnology Co.,Ltd. Address before: 200433 Huang Xing Road, Yangpu District, Shanghai, room 901, room 1675 Patentee before: Shanghai Chen Xiang Medical Equipment Co.,Ltd. |