CN106366209A - Method for extracting polysaccharide from agrocybe cylindracea - Google Patents
Method for extracting polysaccharide from agrocybe cylindracea Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 47
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 47
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 47
- 238000000034 method Methods 0.000 title claims abstract description 23
- 241000908270 Agrocybe cylindracea Species 0.000 title 1
- 238000000605 extraction Methods 0.000 claims abstract description 34
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 28
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims abstract description 27
- 240000003553 Leptospermum scoparium Species 0.000 claims abstract description 27
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 239000000284 extract Substances 0.000 claims abstract description 12
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 11
- 239000002002 slurry Substances 0.000 claims abstract description 11
- 239000002244 precipitate Substances 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 7
- 102000011759 adducin Human genes 0.000 claims abstract description 6
- 108010076723 adducin Proteins 0.000 claims abstract description 6
- 238000009835 boiling Methods 0.000 claims abstract description 6
- 239000012153 distilled water Substances 0.000 claims abstract description 6
- 238000000746 purification Methods 0.000 claims abstract description 6
- 239000000243 solution Substances 0.000 claims description 18
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 10
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 10
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 150000002632 lipids Chemical class 0.000 claims description 4
- 239000000049 pigment Substances 0.000 claims description 4
- 238000004042 decolorization Methods 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000000047 product Substances 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 3
- 238000000227 grinding Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 238000005303 weighing Methods 0.000 abstract 1
- 241000222532 Agrocybe Species 0.000 description 3
- 241000234653 Cyperus Species 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 241000237509 Patinopecten sp. Species 0.000 description 1
- 240000001462 Pleurotus ostreatus Species 0.000 description 1
- 235000001603 Pleurotus ostreatus Nutrition 0.000 description 1
- 229920001231 Polysaccharide peptide Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108010022457 polysaccharide peptide Proteins 0.000 description 1
- 235000021067 refined food Nutrition 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
本发明公开了一种茶树菇提取多糖的方法，本涉及农副产品精深加工技术领域。该方法包括（1）将原料洗净切成小块，烘干后研磨成粉末；（2）原料的复水：称取粉末放入容器中，加适量的水浸泡20min‑35min，得到复水的浆体；（3）提取：在复水的浆体里加入蒸馏水，使浸提比分别为1:10‑30，将容器放入沸水浴中蒸煮1‑3h，然后冷却，将浸提液倒入离心管离心；(4)多糖的纯化；（5）多糖的醇析和干燥：将萃取液倒入容器，加入95%乙醇至50%‑80%饱和度,静置24h，离心后收集沉淀，冷冻干燥得到多糖成品。本发明的方法缩短浸提时间，增加有效成分的提取率，具有工艺简单，易于操作、提取率高等优点。The invention discloses a method for extracting polysaccharides from tea tree mushroom, which relates to the technical field of deep processing of agricultural and sideline products. The method includes (1) washing and cutting the raw material into small pieces, drying and grinding into powder; (2) rehydration of the raw material: weighing the powder into a container, adding an appropriate amount of water to soak for 20min‑35min, and obtaining rehydration (3) Extraction: add distilled water to the rehydrated slurry, so that the extraction ratio is 1:10-30 respectively, put the container in a boiling water bath for 1-3h, then cool, and extract Pour into a centrifuge tube and centrifuge; (4) Purification of polysaccharides; (5) Alcohol analysis and drying of polysaccharides: Pour the extract into a container, add 95% ethanol to 50%‑80% saturation, let stand for 24 hours, and collect after centrifugation Precipitate and freeze-dry to obtain the finished polysaccharide. The method of the invention shortens the extraction time, increases the extraction rate of active ingredients, and has the advantages of simple process, easy operation, high extraction rate and the like.
Description
技术领域technical field
本发明涉及农副产品精深加工技术领域，尤其是涉及一种茶树菇提取多糖的方法。The invention relates to the technical field of intensive processing of agricultural by-products, in particular to a method for extracting polysaccharides from tea tree mushrooms.
背景技术Background technique
多糖是由很多单糖分子通过糖普键链接聚合缩水而成的高分子碳水化合物，真菌多糖一般可以从发酵液、菌丝体和子实体中提取，提取出的多糖具有抗氧化抗肿瘤抗癌等作用。茶树菇多糖主要可以分成葡聚糖、甘露聚糖杂多糖、多糖肤和糖蛋白等。不同分子量、组成的多糖，其有效成分的作用及营养价值也不同。Polysaccharides are high-molecular carbohydrates formed by the polymerization and shrinkage of many monosaccharide molecules through the linkage of glycosides. Fungal polysaccharides can generally be extracted from fermentation broth, mycelia, and fruiting bodies. The extracted polysaccharides have anti-oxidation, anti-tumor, and anti-cancer properties. effect. Tea tree mushroom polysaccharides can be mainly divided into dextran, mannan polysaccharides, polysaccharide peptides and glycoproteins. Polysaccharides with different molecular weights and compositions have different functions and nutritional values of active ingredients.
茶树菇是一种珍稀食用菌，菌肉肥厚，质地脆嫩，特别是菌柄组织致密、结实、乳白，可全部食用，且菌柄比菌盖更脆滑爽口，被誉为“平菇土”、“干贝菇”，具有偷快的杏仁香味和鲍鱼的口感，适合保鲜加工。茶树菇多糖还能降血脂、降胆固醇，茶树菇子实体中含有大量的寡糖，与双歧杆菌共同作用，可以促进肠胃的消化吸收功能，深受人们的喜爱。将其作为添加剂加入到食品中，加工出的食品因为含有多糖，对身体健康极有好处，所以深受欢迎。故无论是茶树菇鲜品还是茶树菇多糖深加工后的产品的市场前景都是非常可观的。Tea tree mushroom is a rare edible fungus with thick flesh and crisp texture, especially the dense, firm and milky stipe tissue, which can be eaten entirely, and the stipe is more crisp and smooth than the cap, known as "oyster mushroom soil". ", "Dried Scallop Mushroom", with a pleasant almond aroma and abalone taste, suitable for fresh-keeping processing. Tea tree mushroom polysaccharides can also reduce blood fat and cholesterol. Tea tree mushroom fruiting bodies contain a large amount of oligosaccharides, which work together with bifidobacteria to promote digestion and absorption of the stomach and are deeply loved by people. It is added to food as an additive, and the processed food is very popular because it contains polysaccharides and is very good for health. Therefore, the market prospects of both fresh tea tree mushroom products and deep-processed tea tree mushroom polysaccharides are very promising.
发明内容Contents of the invention
本发明的发明目的在于：公开了一种茶树菇提取多糖的方法，通过从提取剂的筛选、提取温度的调节、提取方式的选择等方面进行改进，达到提高茶树菇多糖提取率的目的。The purpose of the present invention is to disclose a method for extracting polysaccharides from Agrocybe cyperus, through improving the selection of extractant, the adjustment of extraction temperature, the selection of extraction methods, etc., to achieve the purpose of increasing the extraction rate of polysaccharides from Agrocybe cyperus.
为了实现上述目的，本发明的技术方案如下：In order to achieve the above object, the technical scheme of the present invention is as follows:
一种茶树菇提取多糖的方法，其特征在于包括以下步骤：A method for extracting polysaccharides from tea tree mushroom, characterized in that it comprises the following steps:
（1）将原料洗净切成小块，烘干后研磨成粉末；(1) Wash the raw materials and cut them into small pieces, dry them and grind them into powder;
（2）原料的复水：称取粉末放入容器中，加适量的水浸泡20min-35min，得到复水的浆体；(2) Rehydration of raw materials: Weigh the powder into a container, add an appropriate amount of water to soak for 20min-35min, and obtain a rehydration slurry;
（3）提取：在复水的浆体里加入蒸馏水，使浸提比分别为1:10-30，将容器放入沸水浴中蒸煮1-3h，然后冷却，将浸提液倒入离心管离心，以3500-5000r/min的速度，离心10-15min，然后将上清液加热浓缩，直至滤液体积降为原来的1/4-1/5。(3) Extraction: Add distilled water to the rehydrated slurry, so that the extraction ratio is 1:10-30, put the container in a boiling water bath for 1-3 hours, then cool, pour the extraction solution into a centrifuge tube Centrifuge at a speed of 3500-5000r/min for 10-15min, then heat and concentrate the supernatant until the volume of the filtrate is reduced to 1/4-1/5 of its original volume.
(4)多糖的纯化：将脱色液倒入分液漏斗中，以氯仿∶正丁醇的体积比为3-4：1的混合液为提取剂，按照提取剂与脱色液的重量比为0.5-0.8：1的倍量加入分液漏斗振荡萃取，取上层液，反复萃取2-3次至直到没有乳白色变性蛋白质析出为止；(4) Purification of polysaccharides: Pour the decolorizing solution into a separatory funnel, use a mixed solution with a volume ratio of chloroform:n-butanol of 3-4:1 as the extractant, and set the weight ratio of the extractant to the decolorizing solution as 0.5 -0.8:1 was added to the separatory funnel to shake and extract, and the upper layer was taken, and extracted repeatedly 2-3 times until no milky white denatured protein was precipitated;
（5）多糖的醇析和干燥：将萃取液倒入容器，加入95%乙醇至50%-80%饱和度,静置24h，离心后收集沉淀，冷冻干燥得到多糖成品。(5) Alcohol analysis and drying of polysaccharides: Pour the extract into a container, add 95% ethanol to 50%-80% saturation, let it stand for 24 hours, collect the precipitate after centrifugation, and freeze-dry to obtain the finished polysaccharide.
所述步骤（1）中，茶树菇是在60℃的烘箱中烘干至恒重，经24h后取出研磨。In the step (1), the tea tree mushroom is dried in an oven at 60° C. to a constant weight, and is taken out and ground after 24 hours.
在所述步骤（3）中，在进行离心时，其条件是在转速为3000r/min的条件下离心15min。In the step (3), when performing centrifugation, the condition is to centrifuge for 15 minutes at a rotational speed of 3000 r/min.
所述步骤（3）中，将所述浓缩液加3倍体积的10%H2O2加热脱色。In the step (3), add 3 times the volume of 10% H 2 O 2 to the concentrated solution to decolorize it by heating.
所述步骤（5）中，离心后用丙酮漂洗沉淀两次，除去其中的脂类和色素。In the step (5), after centrifugation, the precipitate is rinsed twice with acetone to remove lipids and pigments therein.
综上所述，由于采用了上述技术方案，本发明的有益效果是：In summary, owing to adopting above-mentioned technical scheme, the beneficial effect of the present invention is:
本发明的方法优选了提取效率高的提取剂，确定了最佳的提取温度，加速多糖粗提物从茶树菇中向溶剂扩散的速度，缩短浸提时间，增加有效成分的提取率，具有工艺简单，易于操作、提取率高等优点。本发明的原料不仅可以采用茶树菇，还可以采用茶树菇的下脚料，从中提取多糖成分,能加强茶树菇的综合利用，提高了茶树菇的附加值，为农民增收提供了一种新的途径。The method of the present invention optimizes the extractant with high extraction efficiency, determines the optimum extraction temperature, accelerates the speed of the polysaccharide crude extract from the tea tree mushroom to diffuse into the solvent, shortens the extraction time, and increases the extraction rate of active ingredients. Simple, easy to operate, high extraction rate and other advantages. The raw material of the present invention can not only adopt tea tree mushroom, but also can use the leftovers of tea tree mushroom to extract polysaccharide components, which can strengthen the comprehensive utilization of tea tree mushroom, increase the added value of tea tree mushroom, and provide a new way for farmers to increase their income .
具体实施方式detailed description
本发明公开了一种茶树菇提取多糖的方法，通过从提取剂的筛选、提取温度的调节、提取方式的选择等方面进行改进，达到提高茶树菇多糖提取率的目的。以下通过具体实施例，对本发明作进一步详述。The invention discloses a method for extracting polysaccharides from tea tree mushroom. The purpose of improving the extraction rate of tea tree mushroom polysaccharides is achieved by improving the screening of extractants, the adjustment of extraction temperature, the selection of extraction methods and the like. Below through specific embodiment, the present invention is described in further detail.
实施例1Example 1
一种茶树菇提取多糖的方法，包括以下步骤：A method for extracting polysaccharides from tea tree mushroom, comprising the following steps:
（1）将原料洗净切成小块，在60℃的烘箱中烘干至恒重，经24h后取出研磨；(1) Wash the raw materials and cut them into small pieces, dry them in an oven at 60°C to constant weight, take them out and grind them after 24 hours;
（2）原料的复水：称取粉末放入容器中，加适量的水浸泡20min-35min，得到复水的浆体；(2) Rehydration of raw materials: Weigh the powder into a container, add an appropriate amount of water to soak for 20min-35min, and obtain a rehydration slurry;
（3）提取：在复水的浆体里加入蒸馏水，使浸提比分别为1:10-30，将容器放入沸水浴中蒸煮2h，然后冷却，将浸提液倒入离心管离心，以3500r/min的速度，离心15min，然后将上清液加热浓缩，直至滤液体积降为原来的1/4，将所述浓缩液加3倍体积的10%H2O2加热脱色。(3) Extraction: Add distilled water to the rehydrated slurry, so that the extraction ratio is 1:10-30 respectively, put the container in a boiling water bath and cook for 2 hours, then cool, pour the extraction solution into a centrifuge tube and centrifuge. Centrifuge at a speed of 3500r/min for 15min, then heat and concentrate the supernatant until the volume of the filtrate is reduced to 1/4 of the original volume, and add 3 times the volume of 10% H 2 O 2 to the concentrated solution for decolorization.
(4)多糖的纯化：将脱色液倒入分液漏斗中，以氯仿∶正丁醇的体积比为3-4：1的混合液为提取剂，按照提取剂与脱色液的重量比为0.5-0.8：1的倍量加入分液漏斗振荡萃取，取上层液，反复萃取2-3次至直到没有乳白色变性蛋白质析出为止；(4) Purification of polysaccharides: Pour the decolorizing solution into a separatory funnel, use a mixed solution with a volume ratio of chloroform:n-butanol of 3-4:1 as the extractant, and set the weight ratio of the extractant to the decolorizing solution as 0.5 -0.8:1 was added to the separatory funnel to shake and extract, and the upper layer was taken, and extracted repeatedly 2-3 times until no milky white denatured protein was precipitated;
（5）多糖的醇析和干燥：将萃取液倒入容器，加入95%乙醇至50%-80%饱和度,静置24h，离心后收集沉淀，离心后用丙酮漂洗沉淀两次，除去其中的脂类和色素，冷冻干燥得到多糖成品。(5) Alcohol analysis and drying of polysaccharides: pour the extract into a container, add 95% ethanol to 50%-80% saturation, let it stand for 24 hours, collect the precipitate after centrifugation, rinse the precipitate twice with acetone after centrifugation, and remove it Lipids and pigments are freeze-dried to obtain polysaccharide products.
实施例2Example 2
一种茶树菇提取多糖的方法，包括以下步骤：A method for extracting polysaccharides from tea tree mushroom, comprising the following steps:
一种茶树菇提取多糖的方法，其特征在于包括以下步骤：A method for extracting polysaccharides from tea tree mushroom, characterized in that it comprises the following steps:
（1）将原料洗净切成小块，在60℃的烘箱中烘干至恒重，经24h后取出研磨，研磨成粉末；(1) Wash and cut the raw materials into small pieces, dry them in an oven at 60°C to constant weight, take them out after 24 hours and grind them into powder;
（2）原料的复水：称取粉末放入容器中，加适量的水浸泡35min，得到复水的浆体；(2) Rehydration of raw materials: Weigh the powder into a container, add an appropriate amount of water to soak for 35 minutes, and obtain a rehydration slurry;
（3）提取：在复水的浆体里加入蒸馏水，使浸提比分别为1:10-30，将容器放入沸水浴中蒸煮1h，然后冷却，将浸提液倒入离心管离心，以5000r/min的速度，离心10min，然后将上清液加热浓缩，直至滤液体积降为原来的1/5。(3) Extraction: Add distilled water to the rehydrated slurry, so that the extraction ratio is 1:10-30, put the container in a boiling water bath for 1 hour, then cool, pour the extraction solution into a centrifuge tube and centrifuge. Centrifuge at a speed of 5000r/min for 10min, then heat and concentrate the supernatant until the volume of the filtrate is reduced to 1/5 of its original volume.
(4)多糖的纯化：将脱色液倒入分液漏斗中，以氯仿∶正丁醇的体积比为3-4：1的混合液为提取剂，按照提取剂与脱色液的重量比为0.5-0.8：1的倍量加入分液漏斗振荡萃取，取上层液，反复萃取2-3次至直到没有乳白色变性蛋白质析出为止；(4) Purification of polysaccharides: Pour the decolorizing solution into a separatory funnel, use a mixed solution with a volume ratio of chloroform:n-butanol of 3-4:1 as the extractant, and set the weight ratio of the extractant to the decolorizing solution as 0.5 -0.8:1 was added to the separatory funnel to shake and extract, and the upper layer was taken, and extracted repeatedly 2-3 times until no milky white denatured protein was precipitated;
（5）多糖的醇析和干燥：将萃取液倒入容器，加入95%乙醇至50%-80%饱和度,静置24h，离心后收集沉淀，冷冻干燥得到多糖成品。(5) Alcohol analysis and drying of polysaccharides: Pour the extract into a container, add 95% ethanol to 50%-80% saturation, let it stand for 24 hours, collect the precipitate after centrifugation, and freeze-dry to obtain the finished polysaccharide.
实施例3Example 3
一种茶树菇提取多糖的方法，包括以下步骤：A method for extracting polysaccharides from tea tree mushroom, comprising the following steps:
（1）将原料洗净切成小块，烘干后研磨成粉末；(1) Wash the raw materials and cut them into small pieces, dry them and grind them into powder;
（2）原料的复水：称取粉末放入容器中，加适量的水浸泡35min，得到复水的浆体；(2) Rehydration of raw materials: Weigh the powder into a container, add an appropriate amount of water to soak for 35 minutes, and obtain a rehydration slurry;
（3）提取：在复水的浆体里加入蒸馏水，使浸提比分别为1:10-30，将容器放入沸水浴中蒸煮1-3h，然后冷却，将浸提液倒入离心管离心，以4250r/min的速度，离心10-15min，然后将上清液加热浓缩，直至滤液体积降为原来的1/4-1/5。(3) Extraction: Add distilled water to the rehydrated slurry, so that the extraction ratio is 1:10-30, put the container in a boiling water bath for 1-3 hours, then cool, pour the extraction solution into a centrifuge tube Centrifuge at a speed of 4250r/min for 10-15min, then heat and concentrate the supernatant until the volume of the filtrate is reduced to 1/4-1/5 of its original volume.
(4)多糖的纯化：将脱色液倒入分液漏斗中，以氯仿∶正丁醇的体积比为3-4：1的混合液为提取剂，按照提取剂与脱色液的重量比为0.5-0.8：1的倍量加入分液漏斗振荡萃取，取上层液，反复萃取3次至直到没有乳白色变性蛋白质析出为止；(4) Purification of polysaccharides: Pour the decolorizing solution into a separatory funnel, use a mixed solution with a volume ratio of chloroform:n-butanol of 3-4:1 as the extractant, and set the weight ratio of the extractant to the decolorizing solution as 0.5 -0.8:1 was added to the separatory funnel to vibrate and extract, the upper layer was taken, and the extraction was repeated 3 times until no milky white denatured protein was precipitated;
（5）多糖的醇析和干燥：将萃取液倒入容器，加入95%乙醇至50%-80%饱和度,静置24h，离心后收集沉淀，离心后用丙酮漂洗沉淀两次，除去其中的脂类和色素，冷冻干燥得到多糖成品。(5) Alcohol analysis and drying of polysaccharides: pour the extract into a container, add 95% ethanol to 50%-80% saturation, let it stand for 24 hours, collect the precipitate after centrifugation, rinse the precipitate twice with acetone after centrifugation, and remove it Lipids and pigments are freeze-dried to obtain polysaccharide products.
本发明实施例1-3中茶树菇多糖的提取得率均在98%以上，本发明的方法操作简单，提取率高。The extraction yields of Agrocybe polysaccharides in Examples 1-3 of the present invention are all over 98%. The method of the present invention is simple to operate and has a high extraction rate.
本发明并不局限于前述的具体实施方式。本发明扩展到任何在本说明书中披露的新特征或任何新的组合，以及披露的任一新的方法或过程的步骤或任何新的组合。The present invention is not limited to the foregoing specific embodiments. The present invention extends to any new feature or any new combination disclosed in this specification, and any new method or process step or any new combination disclosed.
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| CN102911284A (en) * | 2012-11-19 | 2013-02-06 | 江南大学 | Method for preparing agrocybe aegerita fruit body polysaccharide with antioxidant activity |
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN105693873A (en) * | 2014-11-24 | 2016-06-22 | 雷印平 | Domestic fungus polysaccharide extraction technology |
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| US5089481A (en) * | 1987-06-18 | 1992-02-18 | Kureha Kagaku Kogyo Kabushiki Kaisha | Polysaccharides and antiviral drugs containing the same as active ingredient |
| CN102911284A (en) * | 2012-11-19 | 2013-02-06 | 江南大学 | Method for preparing agrocybe aegerita fruit body polysaccharide with antioxidant activity |
| CN104262505A (en) * | 2014-10-29 | 2015-01-07 | 安徽省科普产品工程研究中心有限责任公司 | Method for extracting edible mushroom polysaccharide |
| CN105693873A (en) * | 2014-11-24 | 2016-06-22 | 雷印平 | Domestic fungus polysaccharide extraction technology |
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