CN106361723A - Enteric capsule shell and preparation method thereof - Google Patents
Enteric capsule shell and preparation method thereof Download PDFInfo
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- CN106361723A CN106361723A CN201610779650.XA CN201610779650A CN106361723A CN 106361723 A CN106361723 A CN 106361723A CN 201610779650 A CN201610779650 A CN 201610779650A CN 106361723 A CN106361723 A CN 106361723A
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- sericin
- capsule shell
- solution
- methanamide
- proline
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4816—Wall or shell material
- A61K9/4825—Proteins, e.g. gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4816—Wall or shell material
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
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Abstract
The invention relates to an enteric capsule shell. The enteric capsule shell is prepared from, by mass, 1%-45% of sericin protein, 35%-70% of silk fibroin, 1%-15% of formamide, 2%-30% of ethylene glycol and 2%-25% of proline; natural silk protein good in biocompatibility serves as a raw material, small molecules are added to improve the crystallization property of the material, the natural silk protein can be used for replacing gelatin to serve as the capsule shell material, and the problems caused by capsule shells prepared from the gelatin are solved. It is proved through cell proliferation assays that the obtained capsule shell material can support good growth of cells and is good in cell compatibility and harmless to human body. The silk protein is low in price, capable of obtaining the raw materials easily and easy to purify, cannot carry pathogens common to mammals and is suitable for pharmaceutical materials.
Description
Technical field
The present invention relates to field of pharmaceutical excipients is and in particular to arrive a kind of enteric capsule shell and preparation method thereof.
Background technology
Capsule as a kind of medicine covering material, in addition to covering drug smell moreover it is possible to improve medicine stability, to medicine
There are a certain degree of protective effect, particularly enteric coated capsule, it is possible to increase the bioavailability of medicine, prevent gastric juice for medicine
Destruction, these make it become the main dosage form of medicinal preparation for oral administration.
The material that tradition is widely used in production empty hard capsule at present is gelatin.Gelatin is the Cortex Lycii by cattle and sheep pig etc.
Boil acquisition.Gelatin has much unique property, the gelling of such as gelatin, film property, breast as the primary raw material of capsule
The property changed, caking property etc..These performances are the advantages as capsule material for the gelatin.However, gelatine capsule in the market due to
Source is very different, or even the gelatin that useful loose woman's bark rot does, and causes gelatine capsule quality Control, brings harmful to human to be good for
The risk of health.
The time that food is emptied completely under one's belt is 4-6h, and enteric capsulation need to guarantee not occur through the effect of gastric juice
, after entering intestinal juice, there is disintegrate for a period of time in disintegrate.But gelatin is easily degraded, for enteric coated capsule, need to carry out chemistry friendship
Connection reaction makes capsule dissolubility reduce, and delays the gastric juice disintegrate of gelatine capsule, and the residual of chemical cross-linking agent may affect its life
The thing compatibility.
The problems such as due to some religious problems and bovine spongiform encephalopathy, a lot of countries and regions forbid using mammal system
It is made the production also on gelatine capsule for the gelatin and bring impact.Therefore this patent is considered as the Insecta silkworm silk that silkworms spin silk obtains
Albumen is preparing enteric coated capsule.
Silkworm silk is the liquid spraying when matured silkworm cocoons, the continuous long fibre after solidification, is the mankind using earlier
Animal fiber.Silkworm silk is mainly made up of the waxiness and pigment of sericin, fibroin and outside, and fibroin is topmost composition, accounts for
70%-80%, outside sericin accounts for 19%-28%.The complex that fibroin albumen is made up of heavy chain, light chain and glycoprotein three,
It is a kind of filamentary structure albumen, mainly contains the little side chain amino acid such as glycine, alanine, serine, easily form crystallization
Structure makes it be not readily dissolved in water, is difficult to be degraded by enzyme.Sericin contains hydroxyl, carboxyl and amino etc. and has more highly polar side
The aminoacid of base, accounts for the 70% of total amount, therefore sericin is a kind of water-soluble globular albumen, and structure is similar to gelatin, has good
Film property and gelling, but easily degraded by enzymes.Due to pure silk element, the material degradation speed made of pure silk glue or too
Slowly, or too soon it is difficult to meet the requirement of enteric coated capsule, so this patent considers that both blendings are controlled to obtain degradation speed
Enteric coated capsule material.
Compared to the synthesis Enteric Materials adopting in other patents, the used key component sericin egg in the present invention
In vain, fibroin albumen is natural polymer, has a lot of premium properties, substantial amounts of research shows, sericin and fibroin have simultaneously
Good biocompatibility, is therefore widely used in biomedical materials field, safe to the human body harmless, it is to avoid similar synthetic material
Toxicity to human body cell or rejection.
Content of the invention
It is an object of the invention to provide a kind of enteric capsule shell and preparation method thereof, it can effectively solve the problem that background technology
Present in problem.
For achieving the above object, the present invention provides a kind of following technical scheme: enteric capsule shell, its material bag using
Include: sericin；Fibroin albumen；Methanamide；Ethylene glycol and proline；The mass ratio of described material is: sericin 1-45%；
Fibroin albumen 35-70%；Methanamide 1-15%；Ethylene glycol 2-30% and proline 2-25%.
A kind of preparation method of enteric capsule shell, comprises the following steps: step one: prepares sericin solution；Step 2:
Prepare silk fibroin protein solution；Step 3: configuration Methanamide, proline and glycol water；Step 4: prepare mixed solution；
Step 5: prepare finished product；
Wherein:
Step one: with Cocoon shell or silkworm raw silk as raw material, add deionized water boiling water bath cocoon cooking, centrifuging and taking supernatant obtains
Obtain sericin solution, measure its mass percentage concentration, in refrigerator, cold preservation is standby；
Step 2: with Cocoon shell or silkworm raw silk as raw material, after degumming removes sericin, be dried, using lithium bromide dissolving, go
Ionized water obtains silk fibroin protein solution after dialysing 2 ~ 5 days under conditions of 4 DEG C, measures its mass percentage concentration, cold preservation in refrigerator
Standby；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4: the aqueous solution in the fibroin albumen in step one, the sericin in step 2, step 3 is pressed above-mentioned matter
Amount ratio mix homogeneously, obtains mixed solution；
Step 5: drying and forming-film in the mixed solution injection capsule die in step 4 is initially positioned in baking oven, in 50-100
DEG C condition in bakee 30-120min, be subsequently placed in temperature be 25 DEG C, humidity be 55% constant temperature and humidity under the conditions of cooling 1-10 little
When, obtain enteric capsule shell.
Further, in described step 2, the Best Times of dialysis are 3 ~ 4 days.
Further, in described step 5, optimal stoving temperature is 70-90 DEG C, and the optimal baking time is 60-70min；Most preferably
Cool time is 5-8 hour.
Compared with prior art, the invention has the beneficial effects as follows: with the native fibroin of good biocompatibility as raw material,
Add the crystal property that small molecule improves material, can be used for replacing gelatin as capsule shell material, solve to use gelatin glue softgel shell
Problems.The present invention obtain capsule shell material through cell proliferation experiment prove can the good growth of sertoli cell, with
Cell compatibility is good, harmless.Fibroin price is low, and raw material is easy to get, and is easily purified, and will not carry mammal and have
Cause of disease, be suitable for medicinal materials.
Brief description
Fig. 1 is the process chart of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation description is it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is all other that those of ordinary skill in the art are obtained under the premise of not making creative work
Embodiment, broadly falls into the scope of protection of the invention.
Refer to Fig. 1, the present invention provides a kind of a kind of technical scheme: enteric capsule shell, the material that it uses includes: sericin
Albumen；Fibroin albumen；Methanamide；Ethylene glycol and proline；The mass ratio of described material is: sericin 1-45%；Fibroin albumen
35-70%；Methanamide 1-15%；Ethylene glycol 2-30% and proline 2-25%.
A kind of preparation method of enteric capsule shell, comprises the following steps: step one: prepares sericin solution；Step 2:
Prepare silk fibroin protein solution；Step 3: configuration Methanamide, proline and glycol water；Step 4: prepare mixed solution；
Step 5: prepare finished product；
Wherein:
Step one: with Cocoon shell or silkworm raw silk as raw material, add deionized water boiling water bath cocoon cooking, centrifuging and taking supernatant obtains
Obtain sericin solution, measure its mass percentage concentration, in refrigerator, cold preservation is standby；
Step 2: with Cocoon shell or silkworm raw silk as raw material, after degumming removes sericin, be dried, using lithium bromide dissolving, go
Ionized water obtains silk fibroin protein solution after dialysing 2 ~ 5 days under conditions of 4 DEG C, measures its mass percentage concentration, cold preservation in refrigerator
Standby；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4: the aqueous solution in the fibroin albumen in step one, the sericin in step 2, step 3 is pressed above-mentioned matter
Amount ratio mix homogeneously, obtains mixed solution；
Step 5: drying and forming-film in the mixed solution injection capsule die in step 4 is initially positioned in baking oven, in 50-100
DEG C condition in bakee 30-120min, be subsequently placed in temperature be 25 DEG C, humidity be 55% constant temperature and humidity under the conditions of cooling 1-10 little
When, obtain enteric capsule shell.
Further, in described step 2, the Best Times of dialysis are 3 ~ 4 days.
Further, in described step 5, optimal stoving temperature is 70-90 DEG C, and the optimal baking time is 60-70min；Most preferably
Cool time is 5-8 hour.
There is not disintegrate in this enteric capsule shell 24h in 37 DEG C of hot baths；There is not disintegrate in 6h in 37 DEG C of gastric juice, in intestinal
There is disintegrate within 6h in liquid；By adding small molecule Methanamide, ethylene glycol, proline in the sericin blended solution of fibroin, make silk
Plain sericin produces crosslinked, forming part crystalline texture, adjusts crystalline particle size by adjusting baking temperature with the time, thus adjusting
The disintegration rate of section capsule shells is so as to meet enteric and the insoluble effect of stomach；It is former with the native fibroin of good biocompatibility
Material, adds the crystal property that small molecule improves material, can be used for replacing gelatin as capsule shell material, solves to use gelatin glue capsule
The problems of shell.The present invention obtain capsule shell material through cell proliferation experiment prove can the good growth of sertoli cell,
Good with cell compatibility, harmless.Fibroin price is low, and raw material is easy to get, and is easily purified, and will not carry mammal altogether
Some cause of diseases, are suitable for medicinal materials.
Embodiment one:
Step one, by clean silkworm Bombyx bombycis and deionized water in 1:10 ratio in autoclave sterilization pot steaming and decocting 20min,
Filter cocoon shell and obtain sericin solution；
Step 2, Bombyx bombycis are cleaned drying and are obtained degumming by Cocoon degumming in sodium bicarbonate-carbonate aqueous buffer solution
Silk.By degumed silk: lithium-bromide solution (9.3mol/l)=1:20 dissolving degumed silk, mixed solution is through deionized water in 4 DEG C of condition
Lower dialysis obtains silk fibroin solution in 3 ~ 4 days；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4, fibroin, sericin, Methanamide, ethylene glycol, proline 45:45:8:1:1 in mass ratio is mixed in injection mould；
Step 5, mixed solution is put into after 1h in 75 DEG C of baking ovens, places into temperature and is 25 DEG C, and humidity is 55% constant temperature and humidity bar
Cooling shaping under part, obtains enteric capsule shell.
In this enteric capsule shell gastric juice there is not disintegrate in 6h, and in intestinal juice, disintegration time is about 2h.
Embodiment two:
Step one, by clean silkworm Bombyx bombycis and deionized water in 1:20 ratio in autoclave sterilization pot steaming and decocting 1h, mistake
Filter cocoon shell obtains sericin solution；
Step 2, Bombyx bombycis are cleaned drying and are obtained degumming by Cocoon degumming in sodium bicarbonate-carbonate aqueous buffer solution
Silk.By degumed silk: lithium-bromide solution (9.3mol/l)=1:10 dissolving degumed silk, mixed solution is through deionized water in 4 DEG C of condition
Lower dialysis obtains silk fibroin solution in 3 ~ 4 days；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4, fibroin, sericin, Methanamide, ethylene glycol, proline 77:13:7:2:1 in mass ratio is mixed in injection mould；
Step 5, mixed solution is put into after 2h in 75 DEG C of baking ovens, places into temperature and is 25 DEG C, and humidity is 55% constant temperature and humidity bar
Cooling shaping under part, obtains enteric capsule shell.
In this enteric capsule shell gastric juice there is not disintegrate in 6h, and in intestinal juice, disintegration time is about 4h.
Embodiment three:
Step one, by clean silkworm Bombyx bombycis and deionized water in 1:30 ratio in autoclave sterilization pot steaming and decocting 1h, mistake
Filter cocoon shell obtains sericin solution；
Step 2, Bombyx bombycis are cleaned drying and are obtained degumming by Cocoon degumming in sodium bicarbonate-carbonate aqueous buffer solution
Silk.By degumed silk: lithium-bromide solution (9.3mol/l)=1:10 dissolving degumed silk, mixed solution is through deionized water in 4 DEG C of condition
Lower dialysis obtains silk fibroin solution in 3 ~ 4 days；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4, fibroin, sericin, Methanamide, ethylene glycol, proline 38:39:2:20:1 in mass ratio is mixed injection mould
In；
Step 5, mixed solution is put into after 1h in 75 DEG C of baking ovens, places into temperature and is 25 DEG C, and humidity is 55% constant temperature and humidity bar
Cooling shaping under part, obtains enteric capsule shell.
In this enteric capsule shell gastric juice there is not disintegrate in 6h, and in intestinal juice, disintegration time is about 2h.
Example IV:
Step one, by clean silkworm Bombyx bombycis and deionized water in 1:50 ratio in autoclave sterilization pot steaming and decocting 2h, mistake
Filter cocoon shell obtains sericin solution；
Step 2, Bombyx bombycis are cleaned drying and are obtained degumming by Cocoon degumming in sodium bicarbonate-carbonate aqueous buffer solution
Silk.By degumed silk: lithium-bromide solution (9.3mol/l)=1:7 dissolving degumed silk, mixed solution is through deionized water in 4 DEG C of condition
Lower dialysis obtains silk fibroin solution in 3 ~ 4 days；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4, fibroin, sericin, Methanamide, ethylene glycol, proline 60:15:3:20:2 in mass ratio is mixed injection mould
In；
Step 5, mixed solution is put into after 30min in 75 DEG C of baking ovens, places into temperature and is 25 DEG C, and humidity is 55% constant temperature and humidity
Under the conditions of cooling shaping, obtain enteric capsule shell.
In this enteric capsule shell gastric juice there is not disintegrate in 6h, and in intestinal juice, disintegration time is about 3.5h.
Embodiment five:
Step one, by clean silkworm Bombyx bombycis and deionized water in 1:12 ratio in autoclave sterilization pot steaming and decocting 35min,
Filter cocoon shell and obtain sericin solution；
Step 2, Bombyx bombycis are cleaned drying and are obtained degumming by Cocoon degumming in sodium bicarbonate-carbonate aqueous buffer solution
Silk.By degumed silk: lithium-bromide solution (9.3mol/l)=1:15 dissolving degumed silk, mixed solution is through deionized water in 4 DEG C of condition
Lower dialysis obtains silk fibroin solution in 3 ~ 4 days；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4, fibroin, sericin, Methanamide, ethylene glycol, proline 70:5:1:1:23 in mass ratio is mixed in injection mould；
Step 5, mixed solution is put into after 38min in 90 DEG C of baking ovens, places into temperature and is 25 DEG C, and humidity is 55% constant temperature and humidity
Under the conditions of cooling shaping, obtain enteric capsule shell.
In this enteric capsule shell gastric juice there is not disintegrate in 6h, and in intestinal juice, disintegration time is about 2h.
Embodiment six:
Step one, by clean silkworm Bombyx bombycis and deionized water in 1:15 ratio in autoclave sterilization pot steaming and decocting 40min,
Filter cocoon shell and obtain sericin solution；
Step 2, Bombyx bombycis are cleaned drying and are obtained degumming by Cocoon degumming in sodium bicarbonate-carbonate aqueous buffer solution
Silk.By degumed silk: lithium-bromide solution (9.3mol/l)=1:12 dissolving degumed silk, mixed solution is through deionized water in 4 DEG C of condition
Lower dialysis obtains silk fibroin solution in 3 ~ 4 days；
Step 3: take Methanamide, ethylene glycol and proline, be configured to the aqueous solution containing Methanamide, proline and ethylene glycol, standby
With；
Step 4, fibroin, sericin, Methanamide, ethylene glycol, proline 60:15:1:4:20 in mass ratio is mixed injection mould
In；
Step 5, mixed solution is put into after 38min in 90 DEG C of baking ovens, places into temperature and is 25 DEG C, and humidity is 55% constant temperature and humidity
Under the conditions of cooling shaping, obtain enteric capsule shell.
In this enteric capsule shell gastric juice there is not disintegrate in 6h, and in intestinal juice, disintegration time is about 3h.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, permissible
Understand and can carry out multiple changes, modification, replacement to these embodiments without departing from the principles and spirit of the present invention
And modification, the scope of the present invention be defined by the appended.
Claims (4)
1. a kind of enteric capsule shell, the material that it uses includes: sericin；Fibroin albumen；Methanamide；Ethylene glycol and dried meat ammonia
Acid；It is characterized in that: the mass ratio of described material is: sericin 1-45%；Fibroin albumen 35-70%；Methanamide 1-15%；Second
Glycol 2-30% and proline 2-25%.
2. a kind of preparation method of enteric capsule shell, comprises the following steps: step one: prepare sericin solution；Step 2: system
Standby silk fibroin protein solution；Step 3: configuration Methanamide, proline and glycol water；Step 4: prepare mixed solution；Step
Rapid five: prepare finished product；
It is characterized in that:
S1. in step one: with Cocoon shell or silkworm raw silk as raw material, add deionized water boiling water bath cocoon cooking, centrifuging and taking
Supernatant obtains sericin solution, measures its mass percentage concentration, in refrigerator, cold preservation is standby；
S2. in step 2: with Cocoon shell or silkworm raw silk as raw material, after degumming removes sericin, be dried, using lithium bromide
Dissolving, deionized water obtains silk fibroin protein solution after dialysing 2 ~ 5 days under conditions of 4 DEG C, measures its mass percentage concentration, refrigerator
Middle cold preservation is standby；
S3. in step 3: take Methanamide, ethylene glycol and proline, be configured to the water containing Methanamide, proline and ethylene glycol
Solution, standby；
S4. in step 4: the aqueous solution in the fibroin albumen in step one, the sericin in step 2, step 3 is pressed
Above-mentioned quality, than mix homogeneously, obtains mixed solution；
S5. in step 5: drying and forming-film in the mixed solution injection capsule die in step 4 is initially positioned in baking oven,
Bakee 30-120min in 50-100 DEG C of condition, be subsequently placed in temperature and be 25 DEG C, humidity is cold under the conditions of 55% constant temperature and humidity
But 1-10 hour, obtains enteric capsule shell.
3. a kind of enteric capsule shell according to claim 2 preparation method it is characterised in that: in described step 2 dialyse
Best Times be 3 ~ 4 days.
4. a kind of enteric capsule shell according to claim 2 preparation method it is characterised in that: optimal in described step 5
Stoving temperature is 70-90 DEG C, and the optimal baking time is 60-70min；Optimal cool time is 5-8 hour.
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| CN201610779650.XA CN106361723A (en) | 2016-08-31 | 2016-08-31 | Enteric capsule shell and preparation method thereof |
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| CN201610779650.XA CN106361723A (en) | 2016-08-31 | 2016-08-31 | Enteric capsule shell and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1879609A (en) * | 2006-05-10 | 2006-12-20 | 浙江大学 | A method for preparing silk fibroin Vitamin E contained micro capsule |
| CN101433526A (en) * | 2007-11-13 | 2009-05-20 | 上海慧源植物胶囊股份有限公司 | Non-gelatine enteric hard capsule shell material and method for producing the same |
| WO2011005381A2 (en) * | 2009-06-01 | 2011-01-13 | Trustees Of Tufts College | Vortex-induced silk fibroin gelation for encapsulation and delivery |
| CN104130443A (en) * | 2014-07-11 | 2014-11-05 | 安徽省农业科学院蚕桑研究所 | Preparation method of silkworm natural gel for medical soft capsule shells |
| CN105188684A (en) * | 2013-03-15 | 2015-12-23 | 博纳生命科学有限公司 | Silk-based capsules |
-
2016
- 2016-08-31 CN CN201610779650.XA patent/CN106361723A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1879609A (en) * | 2006-05-10 | 2006-12-20 | 浙江大学 | A method for preparing silk fibroin Vitamin E contained micro capsule |
| CN101433526A (en) * | 2007-11-13 | 2009-05-20 | 上海慧源植物胶囊股份有限公司 | Non-gelatine enteric hard capsule shell material and method for producing the same |
| WO2011005381A2 (en) * | 2009-06-01 | 2011-01-13 | Trustees Of Tufts College | Vortex-induced silk fibroin gelation for encapsulation and delivery |
| CN105188684A (en) * | 2013-03-15 | 2015-12-23 | 博纳生命科学有限公司 | Silk-based capsules |
| CN104130443A (en) * | 2014-07-11 | 2014-11-05 | 安徽省农业科学院蚕桑研究所 | Preparation method of silkworm natural gel for medical soft capsule shells |
Non-Patent Citations (3)
| Title |
|---|
| TATYANA DYAKONOV ET AL.: "Design and Characterization of a Silk-Fibroin-Based Drug Delivery Platform Using Naproxen as a Model Drug", 《JOURNAL OF DRUG DELIVERY》 * |
| 朱正华等: "用丝素蛋白作壁材制备丝素蛋白-维生素E 微胶囊的试验", 《蚕业科学》 * |
| 顾其胜等主编: "《实用生物医学材料科学》", 30 September 2005, 上海科学技术出版社 * |
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