CN106359197A - Treatment method for reducing content of malachite green inside fish bodies - Google Patents
Treatment method for reducing content of malachite green inside fish bodies Download PDFInfo
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- CN106359197A CN106359197A CN201610891298.9A CN201610891298A CN106359197A CN 106359197 A CN106359197 A CN 106359197A CN 201610891298 A CN201610891298 A CN 201610891298A CN 106359197 A CN106359197 A CN 106359197A
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- malachite green
- franch
- singer
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- volvariella volvacea
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention relates to the technical field of aquaculture, and in particular to a treatment method for reducing the content of malachite green inside fish bodies. The treatment method comprises the following steps: fishing fishes of commercial specifications, performing temporary culture treatment on the fished commercial fishes, and selling the fishes in markets, wherein toxin removing adsorption feed consisting of raw materials of red mud, NaC1, soybean peptide powder, cyclolignans and a voluariella volvacea extract is manually fed once a day in the temporary culture process. By adopting the treatment method provided by the invention, the residual amount of the malachite green inside the fish bodies can be reduced, and the commercial fishes can reach edible product safety standards.
Description
Technical field
The present invention relates to technical field of aquaculture and in particular in a kind of reduction fish body malachite green oxalate content process side
Method.
Background technology
Malachite green oxalate is a kind of green crystal with metallic luster, and it is antifungal, is dyestuff again, for a long time,
Fisherman prevents the fish molds of fish, branchiomycosiss, ichthyophthiriasiss etc. with it, and in order that the impaired fish extending life of squama,
Transportation neutralization is deposited in pond, also often using malachite green oxalate.
Malachite green oxalate is poisonous triphenylmethane chemicals, is dyestuff, is also antifungal, bactericidal, parasiticide
Medicine, long-term Use out of range can be carcinogenic, research find, malachite green oxalate enter aquatic animal body in after, fat can be rapidly metabolized to
The leucomalachite green of dissolubility, passeris montani saturati malachite green has potentially carcinogenic, teratogenesis, mutagenic effect, its use in aquaculture
Do not obtain the accreditation of FDA (fda)；According to the regulation of European Union bill 2002/675/ec, animal
Derived food Malachite Green and leucomalachite green residual total amount are limited to 2 μ g/kg；The positive list also clear stipulaties of Japan
Malachite green vestigial must not be detected in import aquatic products；China is in agricultural industry criteria " ny5071- 2002 pollution-free food
Fish medicine usage criteria " in also malachite green oxalate is classified as forbidden drugses, due to not having cheap effective succedaneum, malachite green oxalate is in water
The use produced in cultivation remains incessant after repeated prohibition.
Content of the invention
It is an object of the invention to provide a kind of processing method reducing malachite green oxalate content in fish body.
The technical solution used in the present invention is, a kind of processing method reducing malachite green oxalate content in fish body, to reaching business
The fish of product specification is fished for, and sells through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process,
Manually feed a toxin expelling adsorption feed daily, fed in the ratio of 10-15g/kg, that is, every kg is in feeding 10-15g toxin expelling
Adsorption feed, every morning feeds between 8-9 point, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, does not feed any
Feedstuff；
Wherein, toxin expelling adsorption feed is by red soil 80-100 part, nac10.2-0.3 part, soy peptide powder 1-2 part, ring lignanoid 0.03-
0.05 part, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract 2-5 part, for constitutive material make obtain；
During making, by red soil, nac1, soy peptide powder, ring lignanoid, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract, add water so that water in mixture
Weight ratio after 55-58%, high temperature sterilize, inoculation activation after colloid bacillus cereus, at a temperature of 30～32 DEG C cultivate
36～48 hours, it is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention.
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag, and add it heavy
The water mixing defibrination of 5-6 times of amount, adds the koning trichoderma of serosity gross weight 0.2-0.3% in serosity, digests at 25-27 DEG C
42-46 hour, after ebuillition of heated 2-3 minute, 50-52 DEG C of insulation 4-6 hour, filter to get filtrate, be concentrated into the 1/2 of original volume
Obtain Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract.
The present invention has the beneficial effects that,
(1) present invention reduces the processing method of malachite green oxalate content in fish body by temporarily supporting phase feeding toxin expelling adsorption feed, continuously
Feeding 3-5 days, can promote the malachite green oxalate of fish body accumulation to discharge with feces, reduce the residual quantity of malachite green oxalate in fish body, make business
Product fish reaches safe edible standard；
(2) the toxin expelling adsorbent pair obtaining is made for raw material by red soil, nac1, soy peptide powder, ring lignanoid, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract
Malachite green oxalate has preferable adsorptivity；
(3) the Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract in raw material composition has strong fragrant and delicate flavour, has preferable attractant to Fish；
(4) red soil, nac1, soy peptide powder, ring lignanoid, the fermentation of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract combined inoculation colloid bacillus cereus, can carry
The viscosity of high raw material components, obtains tunning after granulating and drying, soaks and will not be scattered, dissolve up to 18 hours in water.
Specific embodiment
Embodiment 1, a kind of processing method reducing malachite green oxalate content in fish body, catch to the fish reaching commercial specification
Drag for, sell through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process, manually feed once daily
Toxin expelling adsorption feed, is fed in the ratio of 10g/kg, and that is, every kg is in feeding 10g feedstuff of the present invention, between every morning 8-9 point
Feed, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff；
Wherein, toxin expelling adsorption feed is carried by 80 parts of red soil, nac10.2 part, 1 part of soy peptide powder, 0.03 part of ring lignanoid, Volvariella volvacea (Bull.Ex Franch.) Singer． slag
Take 2 parts of thing, obtain for constitutive material making；
During making, by red soil, nac1, soy peptide powder, ring lignanoid, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract, add water so that water in mixture
Weight ratio 55%, after high temperature sterilize, colloid bacillus cereus after inoculation activation, cultivate 36 hours at a temperature of 30 DEG C,
It is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention；
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag, and add 5 times of its weight
Water mixing defibrination, in serosity add serosity gross weight 0.2% koning trichoderma, at 25 DEG C digest 42 hours, ebuillition of heated
After 2 minutes, 50 DEG C are incubated 4 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract.
Fishing for commodity bream intramuscular malachite green oxalate content using the detection of high performance liquid chromatography hplc method is 0.55 μ g/kg, hidden
Color malachite green oxalate 8.9 μ g/kg, processes the commodity bream after fishing for embodiment 1 method, after 3 days, adopts high-efficient liquid phase color again
The malachite green oxalate content in fish tissue is fished in spectrum hplc method detection, and result is malachite green oxalate 0.02 μ g/kg, leucogentian violet
0.053μg/kg；
Fishing for commodity Ctenopharyngodon idellus intramuscular malachite green oxalate content using the detection of high performance liquid chromatography hplc method is 0.48 μ g/kg, hidden
Color malachite green oxalate 8.6 μ g/kg, processes the commodity Ctenopharyngodon idellus after fishing for embodiment 1 method, after 3 days, adopts high-efficient liquid phase color again
The malachite green oxalate content in fish tissue is fished in spectrum hplc method detection, and result does not detect for malachite green oxalate, leucogentian violet
0.042μg/kg；
Fishing for the malachite green oxalate content in commodity muscles of Pseudosciaena crocea using the detection of high performance liquid chromatography hplc method is 0.5 μ g/kg, hidden
Color malachite green oxalate 7.2 μ g/kg processes the commodity Carnis Pseudosciaenae after fishing for embodiment 1 method, after 3 days, adopts efficient liquid phase again
The malachite green oxalate content in fish tissue is fished in the detection of chromatograph hplc method, and result does not detect for malachite green oxalate, leucogentian violet
0.019μg/kg；
Fishing for commodity Siniperca chuatsi intramuscular malachite green oxalate content using the detection of high performance liquid chromatography hplc method is 0.42 μ g/kg, hidden
Color malachite green oxalate 8.0 μ g/kg processes the commodity Siniperca chuatsi after fishing for embodiment 1 method, after 3 days, adopts high-efficient liquid phase color again
The malachite green oxalate content in fish tissue is fished in spectrum hplc method detection, and result does not detect for malachite green oxalate, leucogentian violet
0.022μg/kg；
Fishing for the malachite green oxalate content in commodity carassias auratus muscLe using the detection of high performance liquid chromatography hplc method is 0.53 μ g/kg, hidden
Color malachite green oxalate 6.8 μ g/kg processes the commodity Carassius auratuss after fishing for embodiment 1 method, after 3 days, adopts high-efficient liquid phase color again
The malachite green oxalate content in fish tissue is fished in spectrum hplc method detection, and result is malachite green oxalate 0.013 μ g/kg, leucogentian violet
Do not detect；
Embodiment 2, a kind of processing method reducing malachite green oxalate content in fish body, fish for the fish reaching commercial specification,
Sell through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process, manually feed daily and once arrange
Malicious adsorption feed, is fed in the ratio of 10g/kg, and that is, every kg throws between 8-9 point in feeding 10g feedstuff of the present invention, every morning
Hello, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff；
Wherein, toxin expelling adsorption feed is by 90 parts of red soil, nac10.25 part, 1.5 parts of soy peptide powder, 0.04 part of ring lignanoid, Volvariella volvacea (Bull.Ex Franch.) Singer．
3 parts of slag extract, for constitutive material make obtain；
During making, by red soil, nac1, soy peptide powder, ring lignanoid, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract, add water so that water in mixture
Weight ratio 56%, after high temperature sterilize, colloid bacillus cereus after inoculation activation, cultivate 42 hours at a temperature of 31 DEG C,
It is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention.
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag, and add it heavy
The water mixing defibrination of 5.5 times of amount, adds the koning trichoderma of serosity gross weight 0.5% in serosity, digests 44 hours at 26 DEG C,
After ebuillition of heated 2.5 minutes, 51 DEG C are incubated 5 hours, filter to get filtrate, and are concentrated into the 1/2 of original volume and obtain the extraction of Volvariella volvacea (Bull.Ex Franch.) Singer． slag
Thing.
Embodiment 3, a kind of processing method reducing malachite green oxalate content in fish body, catch to the fish reaching commercial specification
Drag for, sell through temporarily supporting after process, then listing to fishing for the marketable fish obtaining, wherein, in temporary rearing process, manually feed once daily
Toxin expelling adsorption feed, is fed in the ratio of 10g/kg, and that is, every kg is in feeding 10g feedstuff of the present invention, between every morning 8-9 point
Feed, during temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff；
Wherein, toxin expelling adsorption feed is by 100 parts of red soil, nac10.3 part, 2 parts of soy peptide powder, 0.05 part of ring lignanoid, Volvariella volvacea (Bull.Ex Franch.) Singer． slag
Extract 2-5 part, for constitutive material make obtain；
During making, by red soil, nac1, soy peptide powder, ring lignanoid, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract, add water so that water in mixture
Weight ratio 58%, after high temperature sterilize, colloid bacillus cereus after inoculation activation, cultivate 48 hours at a temperature of 32 DEG C,
It is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention.
Wherein, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag, and add it heavy
The water mixing defibrination of 6 times of amount, adds the koning trichoderma of serosity gross weight 0.3% in serosity, digests 46 hours at 27 DEG C, plus
After heat boiling 3 minutes, 52 DEG C are incubated 6 hours, filter to get filtrate, are concentrated into the 1/2 of original volume and obtain Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract.
Claims (4)
1. a kind of processing method reducing malachite green oxalate content in fish body, fishes for the fish reaching commercial specification, to fishing for
The marketable fish obtaining through temporarily support process after, then list sell it is characterised in that
In temporary rearing process, manually feed a toxin expelling adsorption feed daily,
Wherein, toxin expelling adsorption feed is by red soil 80-100 part, nac10.2-0.3 part, soy peptide powder 1-2 part, ring lignanoid 0.03-
0.05 part, Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract 2-5 part for constitutive material make obtain；
During making, by red soil, nac1, soy peptide powder, ring lignanoid, the mixing of Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract, add water so that water in mixture
Weight ratio after 55-58%, high temperature sterilize, inoculation activation after colloid bacillus cereus, at a temperature of 30～32 DEG C cultivate
36～48 hours, it is dried after tunning pelletize, obtain toxin expelling adsorption feed of the present invention.
2. according to claim 1 a kind of reduce fish body in malachite green oxalate content processing method it is characterised in that
Volvariella volvacea (Bull.Ex Franch.) Singer． slag extract is made for raw material with Volvariella volvacea (Bull.Ex Franch.) Singer． slag and obtains, during making, will be broken for Volvariella volvacea (Bull.Ex Franch.) Singer． ground-slag, and add its weight 5-6 times
Water mixing defibrination, adds the koning trichoderma of serosity gross weight 0.2-0.3% in serosity, digests 42-46 hour at 25-27 DEG C
Afterwards, ebuillition of heated 2-3 minute, is incubated 4-6 hour at 50-52 DEG C, filters to get filtrate, be concentrated into original volume 1/3 obtains Volvariella volvacea (Bull.Ex Franch.) Singer．
Slag extract.
3. according to claim 1 and 2 a kind of reduce fish body in malachite green oxalate content processing method it is characterised in that
Toxin expelling adsorption feed is fed in the ratio of 10-15g/kg every time, and every morning feeds between 8-9 point.
4. according to claim 1 and 2 a kind of reduce fish body in malachite green oxalate content processing method it is characterised in that
During temporarily supporting, in addition to feeding a toxin expelling adsorption feed daily, do not feed any feedstuff.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610891298.9A CN106359197A (en) | 2016-10-13 | 2016-10-13 | Treatment method for reducing content of malachite green inside fish bodies |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610891298.9A CN106359197A (en) | 2016-10-13 | 2016-10-13 | Treatment method for reducing content of malachite green inside fish bodies |
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| CN106359197A true CN106359197A (en) | 2017-02-01 |
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| CN201610891298.9A Pending CN106359197A (en) | 2016-10-13 | 2016-10-13 | Treatment method for reducing content of malachite green inside fish bodies |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996008170A1 (en) * | 1994-09-14 | 1996-03-21 | Andrew Boyd French | Aquaculture feeding devices and methods |
| CN1265838A (en) * | 1999-12-10 | 2000-09-13 | 周新 | Process for high-efficienctly preparing grain-saving no-toxicity no-chemicals ingredient of animal fodder and the prepared products |
| CN104171606A (en) * | 2014-07-17 | 2014-12-03 | 滁州市南谯区长江水产良种繁育场 | Balsam pear/yam complex toxin-removing soft-shelled turtle fodder and making method thereof |
| CN104585477A (en) * | 2014-12-11 | 2015-05-06 | 黄南概 | Chinese herbal medicine mixed fermentation feed and preparation method thereof |
| CN105981990A (en) * | 2016-01-07 | 2016-10-05 | 凤阳县兴科农业生态发展有限公司 | Intestine-clearing detox fish feed with palm meal and production method thereof |
-
2016
- 2016-10-13 CN CN201610891298.9A patent/CN106359197A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996008170A1 (en) * | 1994-09-14 | 1996-03-21 | Andrew Boyd French | Aquaculture feeding devices and methods |
| CN1265838A (en) * | 1999-12-10 | 2000-09-13 | 周新 | Process for high-efficienctly preparing grain-saving no-toxicity no-chemicals ingredient of animal fodder and the prepared products |
| CN104171606A (en) * | 2014-07-17 | 2014-12-03 | 滁州市南谯区长江水产良种繁育场 | Balsam pear/yam complex toxin-removing soft-shelled turtle fodder and making method thereof |
| CN104585477A (en) * | 2014-12-11 | 2015-05-06 | 黄南概 | Chinese herbal medicine mixed fermentation feed and preparation method thereof |
| CN105981990A (en) * | 2016-01-07 | 2016-10-05 | 凤阳县兴科农业生态发展有限公司 | Intestine-clearing detox fish feed with palm meal and production method thereof |
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Application publication date: 20170201 |