CN106345220B - Application of an adsorption device in adsorption of volatilized ethidium bromide - Google Patents

Abstract

The present invention relates to the application of an adsorption device in the adsorption of volatilized ethidium bromide, the purpose of which is to provide a device capable of adsorbing ethidium bromide in the process of nucleic acid gel electrophoresis, so as to reduce or eliminate the possibility of EB polluting the laboratory air At the same time, it can improve the utilization rate of agarose gel, ensure the uniformity of the test, help promote the development of biology, medicine, drug design and other fields, and provide guarantee for the safe operation of experimenters; the adsorption device of the present invention includes condensation A box and an adsorption box, the condensation box has upper and lower openings, the bottom of the adsorption box is open, and the top is provided with a top cover, the bottom of the adsorption box is connected to the top of the condensation box, the condensation box includes a condensation layer, and the condensation One side of the layer is provided with a water inlet hole, and the other side is provided with a water outlet hole. The condensation layer is provided with a vent hole through the upper and lower sides; Activated carbon, the adsorption layer is provided with through holes communicating with the vent holes of the condensation layer.

Description

Application of an adsorption device in adsorption of volatilized ethidium bromide

technical field

The invention relates to an adsorption device, in particular to the application of an adsorption device in the adsorption of volatilized ethidium bromide, which is used for the adsorption of ethidium bromide in the process of agarose gel preparation and nucleic acid gel electrophoresis.

Background technique

Gel electrophoresis of DNA and RNA has always been one of the routine experiments in the fields of biology and medicine. Whether it is in the verification of plasmid extraction, the display of PCR results, or the evaluation of the effect of pre-sample preparation for genome sequencing, nucleic acid gel electrophoresis is very important. It is the most direct and effective method to provide experimenters with information such as nucleic acid purity, concentration, and fragment size. Ethidium bromide (EB) is a traditional dye for nucleic acid separation and detection during nucleic acid electrophoresis. After the nucleic acid sample is combined with EB and subjected to agarose gel electrophoresis, it is excited by a standard 302nm ultraviolet light transilluminator and emits an orange-red signal. Nucleic acid-EB can be excited by ultraviolet light to emit fluorescence, and the size and purity of nucleic acid fragments can be observed and determined by shooting with Polaroid film or gel imaging processing system with CCD imaging head.

The use of EB dyes in the process of nucleic acid gel electrophoresis has the advantages of good staining effect, high sensitivity, stable properties, easy storage, low price and easy operation; this technology mainly has the following defects: EB is a highly toxic substance with strong Mutagenesis can easily cause cell canceration and is highly volatile (it will be volatile above 60-70°C) and difficult to degrade. This requires the laboratory using EB to set up a special EB-contaminated area, and perform nucleic acid electrophoresis and imaging tests at the same time. Carry out careful protection, otherwise it will cause harm to the experimenters and even the whole laboratory

When nucleic acid electrophoresis is performed in the laboratory, although the operator wears gloves to avoid the risk of EB contamination to the human body through contact, there is currently no protective measure and protection for the test operator for the EB evaporated in the air during the electrophoresis process. means.

With the development of biomedical technology, there are higher and higher requirements for the ease of use and versatility of DNA and RNA gel electrophoresis. In recent years, countries around the world are working on the possibility of EB substitutes, such as genefinder, syprogreen and other non-toxic, low-toxic fluorescent nucleic acid dyes to replace EB, but these dyes cannot be reused due to their low sensitivity and poor developing effect. Many disadvantages greatly limit its application. Therefore, EB is still the most widely used nucleic acid dye for gel electrophoresis of DNA and RNA in laboratories.

In view of this, it is very necessary to develop a device that can adsorb ethidium bromide to reduce or purify the air and test environment polluted by EB volatilization, and reduce the harm of EB to experimenters.

Contents of the invention

The technical problem to be solved in the present invention is to provide a device capable of adsorbing ethidium bromide in the nucleic acid gel electrophoresis process, while reducing or eliminating EB from polluting the laboratory air, and improving the utilization rate of the agarose gel, ensuring The uniformity of the test is conducive to promoting the development of biology and medicine, drug design and other fields, and providing guarantee for the safe operation of experimenters.

In order to achieve the above object, a kind of adsorption device of the present invention is used in the ethidium bromide that absorbs volatilization, and described adsorption device comprises condensation box and adsorption box, and described condensation box opens up and down, and described adsorption box bottom is opened, and top is provided with There is a top cover, the bottom of the adsorption box is connected to the top of the condensation box, the condensation box includes a condensation layer, one side of the condensation layer is provided with a water inlet hole, and the other side is provided with a water outlet hole, and the condensation layer There are ventilation holes through the upper and lower sides; the adsorption box includes an adsorption layer, and activated carbon wrapped in a gas-permeable film is placed in the adsorption layer, and the adsorption layer is provided with through holes communicating with the ventilation holes of the condensation layer.

Wherein the condensation box and the adsorption box are made of aluminum alloy.

Wherein the condensation layer is also provided with several condensation heads, the condensation heads are conical, and their tips are away from the adsorption box.

Wherein each row of condensing heads is arranged at intervals with each row of ventilation holes.

Wherein the size of the condensation box is the same as that of the adsorption box.

The two sides of the bottom of the condensation box are provided with electrophoresis tank fixing plates, and the electrophoresis tank fixing plates are provided with semicircular fixing holes.

The application of the adsorption device of the present invention in the ethidium bromide of adsorption volatilization is different from the prior art in that the present invention has achieved the following technical effects:

The application of the adsorption device of the present invention in the adsorption of volatilized ethidium bromide, using cheap activated carbon adsorbent, solves the problem of polluting the test environment due to EB volatilization in the process of gel making and electrophoresis, and reduces the EB in the gel electrophoresis test process. Harm to the experimenter; at the same time, after the glue is poured, the glue maker is covered by the adsorption device to seal the glue maker, so as to avoid the volatilization of water in the glue making in an open environment, resulting in changes in the gel concentration, ensuring Trial fidelity.

The present invention will be further described below in conjunction with accompanying drawing.

Description of drawings

Fig. 1 is the structural representation of agarose gel gel maker;

Fig. 2 is the structural representation of ethidium bromide adsorption device of the present invention;

Fig. 3 is the use state figure that the ethidium bromide adsorption device of the present invention is buckled and placed on the gel;

Fig. 4 is a schematic structural view of the ethidium bromide adsorption device of the present invention applied to an electrophoresis device.

Explanation of reference signs: 1-agarose gel-making module; 2-gel-making tray; 3-gel-making comb; 4-side seat of gel-making device; 5-condensation box; Ventilation hole; 9-water inlet hole; 10-water outlet hole; 11-electrophoresis tank fixing plate; 12-fixing hole; 13-top cover.

Detailed ways

The above and other technical features and advantages of the present invention will be described in more detail below with reference to the accompanying drawings and embodiments.

As shown in Figure 2, a kind of adsorption device of the present invention is used in the ethidium bromide that absorbs volatilization, and described adsorption device comprises condensation box 5 and adsorption box 6, and condensation box 5 opens up and down, and adsorption box 6 bottom openings, top A top cover 13 is provided, the bottom of the adsorption box 6 is connected to the top of the condensation box 5, the condensation box 5 includes a condensation layer, one side of the condensation layer is provided with a water inlet 9, and the other side is provided with a water outlet 10, the The condensing layer is provided with a ventilation hole 8 that runs through the upper and lower sides. The adsorption box 6 includes an adsorption layer. Activated carbon wrapped in a gas-permeable film is placed in the adsorption layer. hole.

Example 1: Adsorption and volatilization of EB during the preparation of agarose gel

As shown in FIG. 1 , the agarose gel maker consists of six 60×60 mm square agarose gel making modules 1 and the side seat 4 of the gel maker. When making the gel, heat the prepared agarose with a certain concentration in a microwave oven to melt, and then add an appropriate amount of EB. Pour the agarose gel with EB into the gel maker with the gel tray 2 and the gel comb 3 installed in advance, and then cover the condensation box 5 of the ethidium bromide adsorption device in Figure 2 on the gel maker as shown in Figure 1 on (as shown in Figure 3). The ethidium bromide adsorption device shown in Figure 2 consists of two boxes. One is the condensation box 5, which includes a condensation layer, and the other is the adsorption box 6, which includes an adsorption layer. Both the condensation box and the adsorption box are cuboid boxes of equal size (the cooling box 5; the adsorption box 6 is an activated carbon box) ; The mouth of the condensation box 5 can be tightly buckled on the glue maker (Figure 1). Both the condensation box 5 and the adsorption box 6 are made of aluminum alloy. Water inlet holes 9 and water outlet holes 10 are respectively provided on both sides of the adsorption layer. Before the glue is made, 0°C cold water is firstly added to the condensation layer of the condensation box. A conical condensation head 7 and vent holes 8 are also provided on the condensation layer. During the glue making process, a large amount of water vapor volatilized by the hot glue condenses the water vapor into water under the action of the low temperature of the condensation layer, and most of the water can be condensed into water droplets around the conical condensation head 7 and dripped into the glue maker . The ventilation hole 8 on the condensation layer runs through the condensation layer and reaches the adsorption layer (adsorption box 6), so that the volatilized EB reaches the adsorption layer through the ventilation hole 8; holes, activated carbon wrapped in a breathable film is installed in the adsorption layer of the adsorption box. EB volatilized during the gel making process can pass through the vent holes of the condensation layer, reach the adsorption layer, and be absorbed by the activated carbon in the adsorption layer. According to the ambient temperature of the laboratory, during the gel making process, when the volatilized water vapor is not condensed into water by the condensed layer after passing through the condensed layer, or the condensation effect becomes poor after several days of gel making, and the activated carbon becomes wet, the condensed layer can be replaced at will The cold water and activated carbon in the adsorption layer ensure that the volatilized EB is absorbed as much as possible.

The cold water in the condensation layer and the active carbon in the adsorption layer can be replaced at will.

Example 2: Adsorption and volatilization of EB during nucleic acid electrophoresis

Fig. 4 is the schematic diagram that ethidium bromide adsorption device is used in nucleic acid electrophoresis process, and its installation structure and adsorption principle are identical with agarose gel, and the lid opening size of condensation box 5 is the same as the size of electrophoresis tank, and the plane setting of the lid opening is the same as that of electrophoresis. The tanks are attached, and the electrophoresis tank fixing plate 11 and the semicircular fixing holes 12 fixed with the positive and negative electrodes of the electrophoresis tank are provided on the bonding plane. After adding the electrophoresis sample, fasten the ethidium bromide adsorption device (Figure 4) on the electrophoresis tank so that the fixing plate hole 12 is buckled on the positive and negative electrodes. In this way, the ethidium bromide adsorption device can be simply fixed on the electrophoresis tank, and the airtight adsorption box can condense the volatilized water vapor during the electrophoresis process, and the volatilized EB and other harmful gases are adsorbed by the activated carbon of the adsorption layer. According to the ambient temperature of the laboratory, during the electrophoresis process, when the volatilized water vapor is not condensed into water by the condensation layer after passing through the condensation layer, or the condensation effect becomes poor after several electrophoresis, and the activated carbon becomes wet, the condensation layer can be replaced at will. Cold water and activated carbon in the adsorption layer to ensure that the volatilized EB is absorbed as much as possible.

The above-mentioned embodiments are only descriptions of preferred implementations of the present invention, and are not intended to limit the scope of the present invention. Variations and improvements should fall within the scope of protection defined by the claims of the present invention.

Claims (6)

1. An application of an adsorption device in adsorbing volatilized ethidium bromide, which is characterized in that: the adsorption device comprises a condensation box and an adsorption box, wherein the upper opening and the lower opening of the condensation box are respectively provided with an opening at the bottom of the adsorption box, a top cover is arranged at the top of the adsorption box, the bottom of the adsorption box is communicated with the top of the condensation box, the condensation box comprises a condensation layer, one side of the condensation layer is provided with a water inlet hole, the other side of the condensation layer is provided with a water outlet hole, and the condensation layer is provided with a vent hole penetrating through the upper surface and the lower surface; the adsorption box comprises an adsorption layer, wherein activated carbon wrapped by a breathable film is placed in the adsorption layer, and the adsorption layer is provided with through holes communicated with the ventilation holes of the condensation layer.

2. Use of an adsorption device according to claim 1 for the adsorption of volatilized ethidium bromide, characterized in that: the condensing box and the adsorption box are made of aluminum alloy materials.

3. Use of an adsorption device according to claim 1 for the adsorption of volatilized ethidium bromide, characterized in that: the condensation layer is also provided with a plurality of condensation heads, the condensation heads are conical, and the pointed ends of the condensation heads deviate from the adsorption box.

4. Use of an adsorption device according to claim 3 for the adsorption of volatilized ethidium bromide, characterized in that: each row of condensing heads is arranged at intervals with each row of vent holes.

5. Use of an adsorption device according to claim 1 for the adsorption of volatilized ethidium bromide, characterized in that: the condensation box and the adsorption box are the same in size.

6. Use of an adsorption device according to claim 1 for the adsorption of volatilized ethidium bromide, characterized in that: the electrophoresis tank fixing plates are arranged on two sides of the bottom of the condensation box, and semicircular fixing holes are formed in the electrophoresis tank fixing plates.

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