CN106319021A - Pretreatment method and equipment for bacterial specimens - Google Patents
Pretreatment method and equipment for bacterial specimens Download PDFInfo
- Publication number
- CN106319021A CN106319021A CN201610770947.XA CN201610770947A CN106319021A CN 106319021 A CN106319021 A CN 106319021A CN 201610770947 A CN201610770947 A CN 201610770947A CN 106319021 A CN106319021 A CN 106319021A
- Authority
- CN
- China
- Prior art keywords
- cup
- antibacterial
- specimen
- antibacterial specimen
- crane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002203 pretreatment Methods 0.000 title claims abstract description 9
- 230000001580 bacterial effect Effects 0.000 title abstract 9
- 238000000034 method Methods 0.000 claims abstract description 24
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 230000000844 anti-bacterial effect Effects 0.000 claims description 88
- 238000007781 pre-processing Methods 0.000 claims description 14
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 10
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 3
- 230000035939 shock Effects 0.000 claims description 3
- 244000052616 bacterial pathogen Species 0.000 claims description 2
- 210000001124 body fluid Anatomy 0.000 claims description 2
- 239000010839 body fluid Substances 0.000 claims description 2
- 210000002249 digestive system Anatomy 0.000 claims description 2
- 241000208340 Araliaceae Species 0.000 claims 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims 1
- 235000003140 Panax quinquefolius Nutrition 0.000 claims 1
- 235000008434 ginseng Nutrition 0.000 claims 1
- 206010036790 Productive cough Diseases 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007821 culture assay Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Mechanical Engineering (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Sampling And Sample Adjustment (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a pretreatment method and equipment for bacterial specimens. A lifting frame and a top frame are arranged, rotating devices are arranged on the top frame, a plurality of bacterial specimen cups are put on the lifting frame, the lifting frame can move along a guide rod, so that the bacterial specimen cups are close to the lower parts of the rotating devices, rotating rods of the rotating devices drive rotating caps to rotate, stirring rods connected with the rotating caps are enabled to stir the bacterial specimens in the bacterial specimen cups, and accordingly, the bacterial specimens can be automatically stirred. The pretreatment method and equipment have the advantages that the stirring efficiency is high, large-scale stream-lined bacterial specimen pretreatment processes can be realized, the labor cost is greatly saved, the processing cost of the bacterial specimens is reduced, and higher practicality is realized.
Description
Technical field
The present invention relates to a kind of antibacterial specimen pre-treating method.
Background technology
In hospital or other research institutions, when carrying out the diagnosis of disease, it is often necessary to collected by antibacterial specimen cup
Some sputum or urines etc. are containing germy specimen, then by specimen being carried out culture assays after the pretreatment procedures such as stirring,
Thus the diagnosis for corresponding disease provides evidence.But in prior art, the pre-treatment of antibacterial specimen generally uses and manually carries out
Operation, its efficiency comparison is low, and when needs process substantial amounts of antibacterial specimen, manual operation usually cannot be timely completed, it is difficult to full
Demand during the extensive process of foot.
Summary of the invention
In order to overcome the deficiencies in the prior art, the present invention provides a kind of antibacterial specimen pre-treating method and equipment, and it has
Automation mechanized operation can be realized, feature that efficiency is high, it is possible to achieve the extensive demand processed.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of antibacterial specimen pre-treating method, comprises the following steps: (1) collect specimen, gathers the band of patient with antibacterial specimen cup
The antibacterial specimen of thalline liquid, the antibacterial specimen amount of collection is wanted enough, it is impossible to less than 1ml；(2) in antibacterial specimen cup, digestion is added
Liquid or other corresponding reagent, fixing in being then placed in the cup of antibacterial specimen pre-processing device；(3) locate before starting antibacterial specimen
Reason equipment, on the control panel of antibacterial specimen pre-processing device, input speed, mixing time etc. control parameter；(4) press out
Beginning button, the crane of antibacterial specimen pre-processing device rises, makes the swingle of the rotary apparatus on upper frame stretch into antibacterial specimen
Above the swivelling cover of cup and lid is rotated rotates, thus the antibacterial specimen in antibacterial specimen cup is stirred；(5) cup is taken,
When the mixing time set arrives, swingle stops the rotation, and crane declines, and is taken off by antibacterial specimen cup from cup, thus
Complete the pre-treatment of antibacterial specimen.
As the improvement of technique scheme, in described step (2), the volume of reagent equal with the volume of antibacterial specimen or
Person's difference is less than 20%.
As the further improvement of technique scheme, in described step (3), the rotating speed set as 100～400r/min,
Mixing time is 2～5 minutes.
A kind of antibacterial specimen pre-processing device, including pedestal, described pedestal is provided with control panel, and pedestal top is provided with
Crane and upper frame, base bottom is provided with multiple shock callus, is provided with guide rod, crane between described crane and upper frame
Upper end is provided with multiple cup, is placed with antibacterial specimen cup in described cup, and upper frame is positioned at cup and is provided above respective numbers
Rotary apparatus, crane connect have lift drive mechanism, crane can be upper and lower along guide rod under the driving of lift drive mechanism
Mobile, described antibacterial specimen cup upper end is provided with swivelling cover, and swivelling cover lower end connects the stirring rod for stirring antibacterial specimen,
Rotary apparatus lower end is provided with the swingle coordinated with swivelling cover, and described swingle can be rotated lid and rotate thus to antibacterial mark
Antibacterial specimen in this glass carries out pre-treatment.
The invention has the beneficial effects as follows: a kind of antibacterial specimen pre-treating method of the present invention and equipment, be provided by rising
Fall frame and upper frame, upper frame is provided with rotary apparatus, crane is placed with multiple antibacterial specimen cup, and crane can move along guide rod
Moving and make antibacterial specimen cup below rotary apparatus, then the swingle of rotary apparatus is rotated lid rotation, makes and swivelling cover
Antibacterial specimen in antibacterial specimen cup is stirred by the stirring rod connected, such that it is able to realize antibacterial specimen automatization
The treatment process of stirring, its stirring efficiency is high, can realize extensive, the antibacterial specimen pretreatment procedure of streamline, be greatly saved
Cost of labor, reduces the expense of antibacterial sample disposal, has the practicality that comparison is high.
Accompanying drawing explanation
The present invention is further described with embodiment below in conjunction with the accompanying drawings.
Fig. 1 is the structural representation of the equipment of the present invention.
Detailed description of the invention
A kind of antibacterial specimen pre-treating method, comprises the following steps: (1) collect specimen, gathers patient with antibacterial specimen cup
The antibacterial specimen of the body fluid that carries disease germs (such as sputum or urine), the antibacterial specimen amount of collection is wanted enough, it is impossible to less than 1ml；(2) past
Digestive system or other corresponding reagent is added in antibacterial specimen cup, solid in being then placed in the cup of antibacterial specimen pre-processing device
Fixed；(3) antibacterial specimen pre-processing device is started, on the control panel of antibacterial specimen pre-processing device when input speed, stirring
Between wait control parameter；(4) pressing start button, the crane of antibacterial specimen pre-processing device rises, and makes the rotation on upper frame fill
The swingle put stretches into above the swivelling cover of antibacterial specimen cup and is rotated lid and rotates, thus to the antibacterial in antibacterial specimen cup
Specimen is stirred；(5) taking cup, when the mixing time of setting arrives, swingle stops the rotation, and crane declines, by antibacterial mark
This cup takes off from cup, thus completes the pre-treatment of antibacterial specimen.
Concrete, in described step (2), the volume of reagent should be roughly equal with the volume of antibacterial specimen, and it differs
Big less than 20%.
Further, in described step (3), the rotating speed set is as 100～400r/min, and mixing time is 2～5 minutes,
The difference of the antibacterial specimen according to required process, arranges suitable rotating speed and mixing time.
With reference to Fig. 1, a kind of antibacterial specimen pre-processing device that said method is used, it includes that pedestal 1, pedestal 1 are arranged
Having control panel 11, control panel 11 to be provided with rotating speed, mixing time etc. and control button, pedestal 1 top is provided with crane 2 He
Upper frame 3, is provided with multiple shock callus 12 bottom pedestal 1, be provided with guide rod 4, crane 2 upper end between crane 2 and upper frame 3
Being provided with multiple cup 5, be placed with the antibacterial specimen cup 6 for holding antibacterial specimen in cup 5, antibacterial specimen cup 6 can pass through
Fixing in snapping in cup 5, upper frame 3 is positioned at cup 5 and is provided above the rotary apparatus 7 of respective numbers, and crane 2 connects lifting
Drive mechanism, crane 2 can move up and down along guide rod 4 under the driving of lift drive mechanism, and antibacterial specimen cup 6 upper end is provided with
Swivelling cover, swivelling cover lower end connects the stirring rod for stirring antibacterial specimen, and rotary apparatus 7 lower end is provided with joins with swivelling cover
The swingle 71 closed, before described swingle 71 can be rotated lid rotation thus carry out the antibacterial specimen in antibacterial specimen cup 6
Process.During use, crane 2 can move along guide rod 4 make antibacterial specimen cup 6 below rotary apparatus 7, then rotary apparatus 7
Swingle 71 be rotated lid rotate, make the stirring rod being connected with swivelling cover that the antibacterial specimen in antibacterial specimen cup 6 to be carried out
Stir process, such that it is able to realize the treatment process to the stirring of antibacterial specimen automatization, its stirring efficiency is high, can realize advising greatly
Mould, the antibacterial specimen pretreatment procedure of streamline, be greatly saved cost of labor, reduce the expense of antibacterial sample disposal, tool
There is the practicality that comparison is high.
The above, the simply better embodiment of the present invention, but the present invention is not limited to above-described embodiment, as long as its with
Any same or similar means reach the technique effect of the present invention, within all should being included in protection scope of the present invention.
Claims (4)
1. an antibacterial specimen pre-treating method, it is characterised in that comprise the following steps: (1) collect specimen, uses antibacterial specimen cup
Gathering the antibacterial specimen of the body fluid that carries disease germs of patient, the antibacterial specimen amount of collection is wanted enough, it is impossible to less than 1ml；(2) toward antibacterial specimen
Digestive system or other corresponding reagent is added in cup, fixing in being then placed in the cup of antibacterial specimen pre-processing device；(3) start
Antibacterial specimen pre-processing device, on the control panel of antibacterial specimen pre-processing device, input speed, mixing time etc. control ginseng
Number；(4) pressing start button, the crane of antibacterial specimen pre-processing device rises, and makes the swingle of rotary apparatus on upper frame
Stretch into above the swivelling cover of antibacterial specimen cup and be rotated lid to rotate, thus the antibacterial specimen in antibacterial specimen cup is stirred
Mix；(5) taking cup, when the mixing time of setting arrives, swingle stops the rotation, and crane declines, by antibacterial specimen cup from cup
On take off, thus complete the pre-treatment of antibacterial specimen.
A kind of antibacterial specimen pre-treating method the most according to claim 1, it is characterised in that: in described step (2), reagent
Volume equal with the volume of antibacterial specimen or difference is less than 20%.
A kind of antibacterial specimen pre-treating method the most according to claim 1, it is characterised in that: in described step (3), set
Rotating speed be 100～400r/min, mixing time is 2～5 minutes.
A kind of antibacterial specimen pre-processing device the most according to claim 1, it is characterised in that include pedestal described in:, described
Pedestal is provided with control panel, and pedestal top is provided with crane and upper frame, and base bottom is provided with multiple shock callus, described
Being provided with guide rod between crane and upper frame, crane upper end is provided with multiple cup, is placed with antibacterial specimen in described cup
Cup, upper frame is positioned at cup and is provided above the rotary apparatus of respective numbers, and crane connects lift drive mechanism, and crane can
Moving up and down along guide rod under the driving of lift drive mechanism, described antibacterial specimen cup upper end is provided with swivelling cover, under swivelling cover
End connects the stirring rod for stirring antibacterial specimen, and rotary apparatus lower end is provided with the swingle coordinated with swivelling cover, described
Swingle can be rotated lid rotation thus the antibacterial specimen in antibacterial specimen cup is carried out pre-treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610770947.XA CN106319021A (en) | 2016-08-30 | 2016-08-30 | Pretreatment method and equipment for bacterial specimens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610770947.XA CN106319021A (en) | 2016-08-30 | 2016-08-30 | Pretreatment method and equipment for bacterial specimens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106319021A true CN106319021A (en) | 2017-01-11 |
Family
ID=57788389
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610770947.XA Pending CN106319021A (en) | 2016-08-30 | 2016-08-30 | Pretreatment method and equipment for bacterial specimens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106319021A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490198A (en) * | 2018-03-26 | 2018-09-04 | 郭庆 | A kind of discrimination method of bacterium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1571922A (en) * | 2001-10-19 | 2005-01-26 | 蒙诺根有限公司 | Automated system and method for processing multiple liquid-based specimens |
| CN102304470A (en) * | 2011-08-13 | 2012-01-04 | 王敬华 | Multichannel automatic bacteria rapid inoculator and using method thereof |
| CN202170093U (en) * | 2011-08-17 | 2012-03-21 | 广东省人民医院 | 24-hour urine storage timely mixed container |
| CN204602085U (en) * | 2015-01-19 | 2015-09-02 | 苏州迈迪达医疗器械科技有限公司 | A kind of agitating device detecting ight soil |
-
2016
- 2016-08-30 CN CN201610770947.XA patent/CN106319021A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1571922A (en) * | 2001-10-19 | 2005-01-26 | 蒙诺根有限公司 | Automated system and method for processing multiple liquid-based specimens |
| CN102304470A (en) * | 2011-08-13 | 2012-01-04 | 王敬华 | Multichannel automatic bacteria rapid inoculator and using method thereof |
| CN202170093U (en) * | 2011-08-17 | 2012-03-21 | 广东省人民医院 | 24-hour urine storage timely mixed container |
| CN204602085U (en) * | 2015-01-19 | 2015-09-02 | 苏州迈迪达医疗器械科技有限公司 | A kind of agitating device detecting ight soil |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108490198A (en) * | 2018-03-26 | 2018-09-04 | 郭庆 | A kind of discrimination method of bacterium |
| CN108490198B (en) * | 2018-03-26 | 2021-10-19 | 英特莱检验检测认证(山东)有限公司 | Bacteria identification method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201225970Y (en) | Cell tablet dyeing machine | |
| CN108660074B (en) | Integrated solution for nucleic acid extraction PCR amplification detection | |
| CN106272368A (en) | A kind of the most quickly test tube stopper removing machine device people and take lid method | |
| CN103602582B (en) | Automatic plasmid-extracting device | |
| KR20160065975A (en) | A system and method for processing of biological tissue samples | |
| CN208964916U (en) | A kind of magnetic beads for purifying instrument of enrichment method | |
| CN107643201B (en) | Film type monolayer cell pelleter and pelleter method thereof | |
| CN203855572U (en) | Instrument for extracting active substance of organisms | |
| CN106319021A (en) | Pretreatment method and equipment for bacterial specimens | |
| CN105424432B (en) | Full-automatic Sample pretreatment instrument | |
| CN206876690U (en) | A kind of water quality online analyzer | |
| CN107328602A (en) | A kind of field runoff monitoring cell silt sample sampler | |
| CN101143884B (en) | Nucleic acid automatic extractor | |
| CN113218709A (en) | Beer waste liquid and sodium acetate denitrification rate detect with timing sampling device | |
| CN210221586U (en) | Reation kettle sampling device | |
| CN209613265U (en) | Manipulator for the clamping of bottle centrifuge tubing consumptive material | |
| CN206430961U (en) | Automate phlegm sample pre-treatment device | |
| CN115754177A (en) | A kind of phytoplankton test device and method thereof | |
| CN206177691U (en) | Heavy metal digestion device based on vibrate resolution method | |
| CN205192826U (en) | Organize dyeing machine reagent dropping liquid receiving arrangement | |
| CN208399168U (en) | A kind of soil heavy metal ion extractor | |
| CN209630666U (en) | Crystallization apparatus is used in a kind of production of chemical reagent | |
| CN114935484A (en) | Low-carbon and high-efficiency field rapid enrichment and separation device and method for large-volume water sample | |
| CN206014886U (en) | A kind of bacterium sample pre-processing device | |
| CN105217899B (en) | Sewage-treatment plant based on biological adsorption agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |
|
| RJ01 | Rejection of invention patent application after publication |