CN106311368B - A kind of lossless release unit of drop for microsyringe - Google Patents

Abstract

The invention relates to a non-destructive droplet detachment device for a micro-sampler. The non-destructive detachment device includes a micro-sampling component, a bracket component, a position adjustment component and a super-lyophobic liquid separator. The clamp is fixed on the side plate of the bracket assembly, and the super-lyophobic liquid separator is fixed on the position adjustment rod of the lateral position adjustment assembly. Beneficial effects of the present invention: (1) The liquid dripping position of the present invention is accurate. (2) The present invention is quantitatively accurate. (3) The present invention can meet application requirements in different fields. (4) The present invention can improve operating efficiency. (5) The present invention has a wide range of applications.

Description

A non-destructive droplet detachment device for micro-sampler

technical field

The invention relates to a non-destructive detachment device for liquid droplets used in micro-injectors.

Background technique

Because micro-samplers can achieve high-precision and micro-feeding of liquids, they are often used to absorb and distribute liquids in chemistry, biological test analysis, and fluid dynamics experiments. However, due to the adhesiveness of the needle of the injector to the droplet, for a droplet with a small volume, the gravity of the droplet cannot overcome the adhesion force of the needle to make the droplet detach.

In recent years, various methods such as pressure control and micro-ejection have been applied to the study of micro-droplet detachment. Patent CN204051713U designed a micro-pipetting device, the droplet is separated by a pressurizer installed on the sampler shell, but since there is no liquid suction piston rod in the structure, the liquid suction can only be completed by capillary action, The uptake is limited, it can only be used to dispense droplets of a certain size and multiple aspirations are required for multiple feeds. Patent CN104645489A discloses a micro-volume water dropper, which uses an ejection device to separate droplets. However, since the ejection mechanism will give the droplet a certain initial velocity, it cannot meet the experimental requirements of liquid free fall at a specific height in fluid dynamic analysis.

In engineering, a solid surface with a solid-liquid static contact angle greater than 150° and a rolling angle less than 10° is called a super-lyophobic surface. Because the super-lyophobic surface has little adhesion to the droplet, the droplet cannot infiltrate the super-lyophobic surface. , when the droplet is in contact with the super-lyophobic surface, there is no residual liquid phenomenon, so the low adhesion of the super-lyophobic surface can be used to assist the small-volume droplet at the needle tip of the micro-sampler to detach.

Contents of the invention

The problem to be solved by the present invention is to overcome the deficiency that micro-injectors cannot drop tiny liquid droplets, and to invent a non-destructive droplet detachment device for micro-injectors. The device relies on the low-adhesion properties of the superlyophobic surface to nondestructively detach tiny liquid droplets that adhere to microsampler needles.

A non-destructive droplet detachment device for a micro-sampler of the present invention comprises a micro-sampling assembly, a bracket assembly, a position adjustment assembly and a super-lyophobic liquid separation sheet. The micro-sampling component is a commercially available micro-sampler with a scale mark. The material of the bracket assembly can be metal or plastic, and it is divided into two parts: side plate and bottom plate. It can also be fixed on the bottom plate through the bottom thread to change the working position. The position adjustment component can realize the alignment and feeding of the liquid separation sheet and the droplet by controlling the lateral and longitudinal displacement of the super-lyophobic liquid separation sheet. The super-lyophobic liquid separator must meet the requirements of super-lyophobic performance, that is, the contact angle to the liquid is greater than 150°, and the rolling angle is less than 10°; it must have certain pores matching the droplet size and be fixed on the position adjustment component.

Technical scheme of the present invention:

A non-destructive droplet detachment device for a micro-sampler, the non-destructive droplet detachment device includes a micro-sampling assembly 1, a bracket assembly 15, a position adjustment assembly and a super-lyophobic liquid separator 4, the micro-sampling assembly 1 passes through The elastic clamp 2 is fixed on the side plate 14 of the bracket assembly 15, and the super-lyophobic liquid separator 4 is fixed on the lateral position adjustment rod 8 of the lateral position adjustment mechanism 12;

The bracket assembly 15 includes a side plate 14, a bottom plate 6 and an elastic clamp 2; the side plate 14 is provided with a chute that restricts the overall sliding of the lateral position adjustment mechanism 12 and a through hole that matches the cam adjustment lever 7;

The position adjustment assembly includes a lateral position adjustment mechanism 12 and a longitudinal position adjustment mechanism 10; the lateral position adjustment mechanism 12 controls the opening 5 on the super-lyophobic liquid separation sheet 4 to align with the needle of the micro-sampling assembly 1, The lateral position adjustment mechanism 12 includes an integral slider 11, a lateral position adjustment rod 8 and a locking magnet block 13. The overall slider 11 is located in the chute of the side plate 14, and is used to limit the overall sliding of the lateral position adjustment mechanism 12. The lateral position adjustment rod 8 is inserted into the hole of the overall slider 11, and the position is locked by the locking magnet block 13; the horizontal position adjustment mechanism 12 is supported by the longitudinal position adjustment mechanism 10, and the vertical position adjustment mechanism 10 controls the superposition by adjusting the height of the horizontal position adjustment mechanism 12 The height of the lyophobic liquid-separating sheet 4 is such that the micro-droplets on the needle of the micro-injection assembly 1 are separated by the super-lyophobic liquid-separating sheet 4; the longitudinal position adjustment mechanism 10 includes a height that controls the overall height of the lateral position adjustment mechanism 12 Adjust the cam 9 and the cam adjustment lever 7;

The super-lyophobic liquid-separating sheet 4 is provided with a fixed-volume opening 5 for detaching liquid droplets and a liquid-separating sheet limiting hole 3 connected to the lateral position adjusting rod 8 .

The height adjustment cam 9 is in a fast stroke when it is away from the needle of the sampler, and is in a slow stroke when it is close to the needle of the sampler.

The materials of the side plate 14, the bottom plate 6 and the longitudinal position adjustment mechanism 10 are metal or plastic;

The material of the lateral position adjustment mechanism 12 is ferromagnetic metal.

Beneficial effects of the present invention:

(1) The dripping position of the present invention is accurate. It can realize the feeding after the precise clamping and positioning of the sample injection component, and can make the liquid drop drop on the target position accurately.

(2) The present invention is quantitatively accurate. Utilizing the super-lyophobic properties of the material, the contact dripping method can ensure that the discharged quantitative liquid can be dripped accurately and without damage.

(3) The present invention can meet application requirements in different fields. By controlling the longitudinal displacement adjustment mechanism, it can ensure that the droplet has no initial velocity when it falls, which not only meets the quantitative requirements in the field of biochemistry, but also can be applied in the field of fluid dynamics analysis.

(4) The present invention can improve operating efficiency. Due to the adoption of the piston-rod suction method of the common sampler, it can achieve large-capacity suction under the premise of ensuring the accuracy of liquid discharge, so as to realize one-time suction and multiple drips.

(5) The present invention has a wide range of applications. Through the in-depth utilization of ordinary sample injectors through auxiliary devices, the quantitative dripping of water droplets/oil droplets can be realized by processing super-lyophobic/super-oleophobic liquid separators respectively.

Description of drawings

Fig. 1 is a schematic diagram of the droplet detachment device of the present invention.

Fig. 2 is a structural schematic diagram of the longitudinal position adjustment mechanism and the lateral position adjustment mechanism.

Figure 3 is a schematic diagram of a super-lyophobic liquid separator.

In the figure: 1 micro-sampling component; 2 elastic clamp; 3 limit hole of liquid separator; 4 super-lyophobic liquid separator;

5 fixed volume opening; 6 bottom plate; 7 cam adjustment lever; 8 lateral position adjustment lever; 9 height adjustment cam;

10 longitudinal position adjustment mechanism; 11 integral slider; 12 lateral position adjustment mechanism;

13 locking magnet blocks; 14 side panels; 15 bracket components.

Detailed ways

The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

Example 1

A droplet detachment device for a micro-sampler is shown in FIG. 1 . Figure 2 shows the local structure of the position adjustment assembly. In this example, water droplets are used as the operating object, and an ordinary micro-sampler with a volume range of 10 μL is used as the sample injection component. The super-lyophobic liquid separator used is an aluminum sheet with a contact angle with water of 160° and a rolling angle of 2°. (6061 aluminum alloy), with pores for 1-8 μL and 4-8 μL drops respectively, as shown in Figure 3. In this embodiment, the side plate and the base plate are fixed to drip liquid.

After the side panels of the bracket assembly are connected to the base plate, place the base plate horizontally. After the sample injection component absorbs 10 μL of water in the full scale, it is fixed on the clamp on the side plate of the bracket. Push the horizontal position adjustment lever, and use the scale mark on the super-lyophobic separator to align the 4-8μL drop hole with the center of the needle of the sampling component and lock it with a magnet block. The longitudinal initial position of the super-lyophobic separator is above the needle port. Push the piston rod of the sample injection component to discharge 5 μL of liquid according to its scale mark. At this time, the droplet will stay at the needle port due to the adhesion force due to too small gravity. Slowly turn the vertical position adjustment lever to move the super-lyophobic separator sheet down. When it reaches the injection needle mouth, it will take away the discharged 5 μL droplet, which means the feeding of 5 μL micro-droplet is completed.

After returning the super-lyophobic separatory sheet to its original height, remove the positioning magnet block, align the 1-8 μL drip hole with the needle of the sampling component using the scale marks on the separatory sheet, and then lock it. Push the piston rod of the sample injection component to discharge 2 μL of liquid according to its scale mark, and the liquid drop will also stay at the needle port at this time. Slowly turn the vertical position adjustment lever to take away the discharged 2μL droplet, that is, through one suction, the feed of micro-droplets with different volumes can be completed multiple times.

Claims (3)

1. the lossless release unit of a kind of drop for microsyringe, which is characterized in that the lossless release unit of the drop includes Micro-sampling component (1), bracket component (15), position adjusting part and super lyophoby liquid separation piece (4), micro-sampling component (1) are logical It crosses spring chuck (2) to be fixed on the side plate (14) of bracket component (15), super lyophoby liquid separation piece (4) is fixed on lateral position adjusting On the lateral position adjusting rod (8) of mechanism (12)；

The bracket component (15) includes side plate (14), bottom plate (6) and spring chuck (2)；Side plate (14) is lateral equipped with limitation The sliding slot of position adjusting mechanism (12) whole slide and the through-hole of matching cam adjustment bar (7)；

The position adjusting part includes lateral position regulating mechanism (12) and lengthwise position regulating mechanism (10)；The horizontal stroke The syringe needle of trepanning (5) alignment micro-sampling component (1) on position adjusting mechanism (12) control super lyophoby liquid separation piece (4), it is horizontal Include whole sliding block (11), lateral position adjusting rod (8) and locking magnet block (13), whole sliding block to position adjusting mechanism (12) (11) in the sliding slot of side plate (14), for limiting lateral position regulating mechanism (12) whole slide, lateral position adjusting rod (8) it is inserted into the hole of whole sliding block (11), and passes through locking magnet block (13) latched position；Lateral position regulating mechanism (12) by Lengthwise position regulating mechanism (10) supports, and lengthwise position regulating mechanism (10) is by adjusting the height of lateral position regulating mechanism (12) Degree, and then the height of super lyophoby liquid separation piece (4) is controlled, make the microlayer model on micro-sampling component (1) syringe needle by super lyophoby liquid separation Piece (4) is detached from；Height of the lengthwise position regulating mechanism (10) including controlling lateral position regulating mechanism (12) whole height Degree adjusts cam (9) and cam adjustment bar (7)；

Be provided on the super lyophoby liquid separation piece (4) make drop be detached from determine volume trepanning (5) and with lateral position adjusting rod (8) Connected liquid separation piece limit hole (3).

2. the lossless release unit of drop according to claim 1, which is characterized in that the height adjusts cam (9) and exists In fast stroke during far from injector syringe needle, idle stroke is in when close to injector syringe needle.

3. the lossless release unit of drop according to claim 1 or 2, which is characterized in that the side plate (14), bottom plate (6) and the material of lengthwise position regulating mechanism (10) is metal or plastics, and described lateral position regulating mechanism (12) material is Ferromagnetic metal.