CN106305792A - Clonostachys rosea-bacillus subtilis composite wettable powder - Google Patents
Clonostachys rosea-bacillus subtilis composite wettable powder Download PDFInfo
- Publication number
- CN106305792A CN106305792A CN201610331376.XA CN201610331376A CN106305792A CN 106305792 A CN106305792 A CN 106305792A CN 201610331376 A CN201610331376 A CN 201610331376A CN 106305792 A CN106305792 A CN 106305792A
- Authority
- CN
- China
- Prior art keywords
- powder
- bacillus subtilis
- parts
- wettable powder
- bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000004563 wettable powder Substances 0.000 title claims abstract description 33
- 239000002131 composite material Substances 0.000 title abstract 3
- 241000142531 Clonostachys Species 0.000 title abstract 2
- 239000000843 powder Substances 0.000 claims abstract description 36
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 230000001580 bacterial effect Effects 0.000 claims abstract description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 16
- 230000002538 fungal effect Effects 0.000 claims abstract description 14
- 241001149472 Clonostachys rosea Species 0.000 claims abstract description 12
- 210000004666 bacterial spore Anatomy 0.000 claims abstract description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 7
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 7
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 7
- 210000004215 spore Anatomy 0.000 claims description 18
- 244000007853 Sarothamnus scoparius Species 0.000 claims description 13
- 238000013329 compounding Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 claims description 7
- 208000031888 Mycoses Diseases 0.000 claims description 5
- JHJUUEHSAZXEEO-UHFFFAOYSA-M sodium;4-dodecylbenzenesulfonate Chemical compound [Na+].CCCCCCCCCCCCC1=CC=C(S([O-])(=O)=O)C=C1 JHJUUEHSAZXEEO-UHFFFAOYSA-M 0.000 claims description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 241001530056 Athelia rolfsii Species 0.000 claims description 2
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 claims description 2
- 208000008469 Peptic Ulcer Diseases 0.000 claims description 2
- 239000003292 glue Substances 0.000 claims description 2
- 208000019802 Sexually transmitted disease Diseases 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 239000002994 raw material Substances 0.000 claims 1
- 201000010099 disease Diseases 0.000 abstract description 20
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 20
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 abstract description 7
- 238000001228 spectrum Methods 0.000 abstract description 2
- 241000221662 Sclerotinia Species 0.000 abstract 1
- 230000003042 antagnostic effect Effects 0.000 abstract 1
- 229920003123 carboxymethyl cellulose sodium Polymers 0.000 abstract 1
- 229940063834 carboxymethylcellulose sodium Drugs 0.000 abstract 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 abstract 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 abstract 1
- 229920005552 sodium lignosulfonate Polymers 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 21
- 239000000575 pesticide Substances 0.000 description 18
- 238000003556 assay Methods 0.000 description 12
- 230000002265 prevention Effects 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 230000000813 microbial effect Effects 0.000 description 10
- 239000000203 mixture Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 238000002156 mixing Methods 0.000 description 8
- 239000002689 soil Substances 0.000 description 8
- 239000007921 spray Substances 0.000 description 8
- 239000000725 suspension Substances 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 6
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 6
- 238000009736 wetting Methods 0.000 description 6
- 230000003902 lesion Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000227653 Lycopersicon Species 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 239000003905 agrochemical Substances 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 208000035143 Bacterial infection Diseases 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 239000006002 Pepper Substances 0.000 description 3
- 241000722363 Piper Species 0.000 description 3
- 235000016761 Piper aduncum Nutrition 0.000 description 3
- 235000017804 Piper guineense Nutrition 0.000 description 3
- 235000008184 Piper nigrum Nutrition 0.000 description 3
- 241000589771 Ralstonia solanacearum Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000010533 azeotropic distillation Methods 0.000 description 3
- 230000000443 biocontrol Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 241000193755 Bacillus cereus Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- -1 Sodium alkyl benzene Chemical class 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229940077388 benzenesulfonate Drugs 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000003628 erosive effect Effects 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- 238000009335 monocropping Methods 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 239000005867 Iprodione Substances 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- VMFXMTJCTSYHCF-HHQUSWFZSA-N [(2r,3r,4s,5r)-5-(hexylamino)-4-hydroxy-2-(hydroxymethyl)-6-[(7-hydroxy-4-oxo-1,3a,5,6,7,7a-hexahydroimidazo[4,5-c]pyridin-2-yl)amino]oxan-3-yl] carbamate Chemical compound CCCCCCN[C@@H]1[C@H](O)[C@@H](OC(N)=O)[C@@H](CO)OC1\N=C\1NC(C(=O)NCC2O)C2N/1 VMFXMTJCTSYHCF-HHQUSWFZSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- ONUFESLQCSAYKA-UHFFFAOYSA-N iprodione Chemical compound O=C1N(C(=O)NC(C)C)CC(=O)N1C1=CC(Cl)=CC(Cl)=C1 ONUFESLQCSAYKA-UHFFFAOYSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000009336 multiple cropping Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 238000003971 tillage Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides clonostachys rosea-bacillus subtilis composite wettable powder containing clonostachys rosea fungus conidial powder for preventing and treating gray mold, sheath blight, root rot, fungal leaf spot and sclerotinia rot and bacillus subtilis bacterial spore powder for preventing and treating bacterial wilt, soft rot diseases, canker, bacterial spot diseases and bacterial angular leaf spot diseases. The wettable powder comprises, by weight, 50-100 parts of clonostachys rosea fungus conidial powder, 50-100 parts of bacillus subtilis bacterial spore powder, 800-850 parts of diatomite, 10-20 parts of sodium dodecyl benzene sulfonate, 10-50 parts of sodium lignosulfonate, 10-50 parts of carboxymethylcellulose sodium and 5-10 parts of ascorbic acid. The wettable powder composite microbes do not produce antagonistic action, have a wide sterilization spectrum, are safe for crops, users and an environment, improve crop quality and improve a yield and performance stability.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of have preventing and treating fungal disease viscous broom mould
(Gliocladium roseum) fungal spore powder and the bacillus subtilis (Bacillus with preventing and treating bacterial disease
Subtilis) method of the wettable powder that bacterial spore powder is compounding.
Background technology
Due to life-time service chemical fertilizer, chemical pesticide, and high intensity multiple cropping, the tillage method of continuous cropping production, cause
Occurring in that in China's agricultural production process that such as pest and disease damage resistance strengthens, soil continuous production ability and utilization rate of fertilizer reduce,
Farmland quality suffers that destruction, organic deficiency, soil compaction, fertility decline, and soil-borne disease and continuous cropping obstacle take place frequently, agricultural production quality
The problem of a series of restriction China's agricultural development such as amount safety problem is the most serious.And by having the microorganism fungus kind of biological and ecological methods to prevent plant disease, pests, and erosion function
The microbial manure prepared by biofermentation technique and formulation technology and microbial pesticide, due to have diseases prevention, growth-promoting,
The functions such as rehabilitating soil Tiny ecosystem, improvement Soil structure, dissolving phosphor and dissolving potassium, raising utilization rate of fertilizer, can effectively solve above-mentioned
The problem of restriction China's agricultural development.
Along with biofermentation technique and the development of formulation technology, China's microbial manure industry and microbial pesticide produce
Industry have also been obtained fast development, especially in nearest twenty or thirty year, microbial manure and the product category of microbial pesticide,
Year output and manufacturing enterprise's quantity are greatly improved, and when the two industry is fast-developing, there is also controlling disease phase
The problems such as relative narrower, protection effect has much room for improvement, strain is easily degenerated, environmental factor dependence is strong are composed in single, preventing and treating, at present in agriculture
Microbial manure center, industry portion and the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC registration microbial manure and microbial pesticide in single culture, list
The product accounting of one function is more than 80%, and the wettable powder of the present invention is prevented and treated microbial inoculum and the moistening of mechanism by two kinds of differences
Agent, dispersant, uv-protector, carrier process, and have preferable suspended dispersed performance and water solublity, both can prevent and treat leaf
Portion's disease can also prevent and treat the disease in soil, and application process is versatile and flexible, root drip irrigation, punching can be used to execute, pouring root and on the ground
The application processes such as portion's spraying, spray, are respectively provided with good prevention effect to multiple fungus and bacteriosis.
Summary of the invention
The invention provides one and have that to prevent and treat gray mold, banded sclerotial blight, root rot, fungal leaf spot, sclerotiniose etc. true
The viscous broom mould fungal spore powder of fungal disease and having prevents and treats bacterial wilt, soft rot, Peptic Ulcers, bacterial speck, bacillary
Wettable powder that the bacillus subtilis bacterial spore powder of the bacterial diseases such as angular leaf spot is compounding and preparation method thereof.
Above-mentioned wettable powder be by viscous broom mould fungal spore powder, bacillus subtilis bacterial spore powder, kieselguhr, 12
Sodium alkyl benzene sulfonate, sodium lignin sulfonate, sodium carboxymethyl cellulose, ascorbic acid are mixed and processed, and count by weight, respectively
Component is respectively as follows: 50-100 part and glues broom mould fungal spore powder, 50-100 part bacillus subtilis bacterial spore powder, 800-850 part
Kieselguhr, 10-20 part dodecylbenzene sodium sulfonate, 10-50 part sodium lignin sulfonate, 10-50 part sodium carboxymethyl cellulose, 5-10
Part ascorbic acid.
The preparation method of described wettable powder is:
Step one, it is that (purity is more than 90% to the 5-10 hundred million viscous broom mould fungal spore powder of cfu/ gram, and water content is less than by concentration
10%, chlamydospore ratio is more than 30%), concentration be the 200-300 hundred million bacillus subtilis bacterial spore powder (purity of cfu/ gram
More than 90%, water content is less than 10%) it is sequentially added in mixer with kieselguhr, after mixing 0.5～1h, sequentially add 12
Sodium alkyl benzene sulfonate, sodium lignin sulfonate, sodium carboxymethyl cellulose, ascorbic acid, mixed after mixing 0.5～1h uniformly
Thing；
Step 2, the mixture of previous step is proceeded to low-temperature airflow pulverizer, pulverize temperature and control below 20 DEG C, pulverize
Rear mistake 300 mesh sieve, i.e. prepares dispersibility and the good wettable powder of suspension.
Above-mentioned Gliocladium roseum bacterial strain (Gliocladium roseum), numbered QGSS-C16, it is isolatable from Shouguang, Shandong
Dong Zhenguan village of city Fructus Lycopersici esculenti plastic shed soil.This bacterial strain was preserved in Chinese microorganism strain preservation management and has entrusted on 04 25th, 2016
Member's meeting common micro-organisms center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), preservation registration number is CGMCC No.12243.
It is PDA culture medium that bacterial strain is suitable for the culture medium of growth, and the pH being suitable for growth is 6.0～6.8, and the temperature being suitable for growth is 25～28
℃。
Above-mentioned bacillus subtilis strain (Bacillus subtilis), numbered QGSS-05, it is isolatable from Shouguang, Shandong
Ternary Zhu Cun.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on October 30th, 2015
Center (Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), preservation registration number is CGMCC No.11563.Bacterial strain is suitable for growth
Culture medium is NA culture medium, and the pH being suitable for growth is 7.0～7.2, and the temperature being suitable for growth is 28～30 DEG C.
What the present invention provided has the wettable powder energy suspended dispersed quick, uniform of disease prevention growth-promoting in water, this
Bright wettable powder has the special feature that and advantage is:
(1) biocontrol fungi of the most not antagonism is compounded with antibacterial, play good potentiation, expand and disease is prevented
Control scope, solve the problem that single bio-control factors diseases prevention spectrum is narrow, it is ensured that the prevention effect to dissimilar disease；
(2) application process is versatile and flexible, root drip irrigation, punching can be used to execute, the side of using such as pouring root and overground part spraying, spray
Method；
(3) Gliocladium roseum added and bacillus subtilis are all that the higher biological and ecological methods to prevent plant disease, pests, and erosion that has of isolated is lived from soil
Property biocontrol bacterial strain, there is strong stress resistance, nutritional requirement is simple, be prone to surely grow, to various crop at plant rhizosphere and blade face
Bacillary and fungal disease has the advantages such as preferable prevention effect；
(4) in the viscous mould spore powder of broom, chlamydosporic ratio, more than 30%, substantially increases the time-to-live of fungal spore,
Extend shelf life；
(5), during bacterial strain uses therefor derives from soil, to crop, personnel, environmental safety height are used.
Detailed description of the invention
Embodiment 1
Step one, by concentration be 1,000,000,000 cfu/ gram 100 parts of viscous broom mould fungal spore powder, concentration be 30,000,000,000 cfu/ gram
50 parts of bacillus subtilis bacterial spore powder and 815 parts of kieselguhr are sequentially added in mixer, after mixing 0.5～1h, more successively
Add dodecylbenzene sodium sulfonate 10 parts, sodium lignin sulfonate 10 parts, sodium carboxymethyl cellulose 10 parts, 5 parts of ascorbic acid, mixing
Uniform mixture is obtained after 0.5～1h；
Step 2, the mixture of previous step is proceeded to low-temperature airflow pulverizer, pulverize temperature and control below 20 DEG C, pulverize
Rear mistake 300 mesh sieve, i.e. prepares dispersibility and the good wettable powder of suspension.
Suspensibility is measured according to 4.1 in agricultural-chemical suspension rate assay method GB/T 14825-2006, and wetting time is pressed
Being measured according to pesticide wettable wettability determination method GB/T 5451-2001, fineness is according to pesticide powder, wettability
2.2 wet screenings in powder fineness assay method GB/T 16150-1995 are measured, and moisture is according to pesticide moisture assay method
In GB/T 1600-2001,2.2 azeotropic distillation methods are measured, and pH value is according to the assay method GB/T 1601-of pesticide pH value
1993 are measured.The suspensibility of this wettable powder is 78% after testing, and wetting time is 83s, and fineness 99% is by 48 μm
Sieve, moisture 6.3%, pH6.9.
Embodiment 2
Step one, by concentration be 1,000,000,000 cfu/ gram 50 parts of viscous broom mould fungal spore powder, concentration be 30,000,000,000 cfu/ gram withered
50 parts of bacillus cereus bacterial spore powder of grass is sequentially added in mixer with 835 parts of kieselguhr, after mixing 0.5～1h, then adds successively
Enter dodecylbenzene sodium sulfonate 15 parts, sodium lignin sulfonate 15 parts, sodium carboxymethyl cellulose 25 parts, 10 parts of ascorbic acid, mixing
Uniform mixture is obtained after 0.5～1h；
Step 2, the mixture of previous step is proceeded to low-temperature airflow pulverizer, pulverize temperature and control below 20 DEG C, pulverize
Rear mistake 300 mesh sieve, i.e. prepares dispersibility and the good wettable powder of suspension.
Suspensibility is measured according to 4.1 in agricultural-chemical suspension rate assay method GB/T 14825-2006, and wetting time is pressed
Being measured according to pesticide wettable wettability determination method GB/T 5451-2001, fineness is according to pesticide powder, wettability
2.2 wet screenings in powder fineness assay method GB/T 16150-1995 are measured, and moisture is according to pesticide moisture assay method
In GB/T 1600-2001,2.2 azeotropic distillation methods are measured, and pH value is according to the assay method GB/T 1601-of pesticide pH value
1993 are measured.The suspensibility of this wettable powder is 75% after testing, and wetting time is 80s, and fineness 99% is by 48 μm
Sieve, moisture 6.8%, pH6.7.
Embodiment 3
Step one, by concentration be 500,000,000 cfu/ gram 100 parts of viscous broom mould fungal spore powder, concentration be 20,000,000,000 cfu/ gram withered
100 parts of bacillus cereus bacterial spore powder of grass is sequentially added in mixer with 815 parts of kieselguhr, after mixing 0.5～1h, then adds successively
Enter dodecylbenzene sodium sulfonate 20 weight portion, sodium lignin sulfonate 20 weight portion, sodium carboxymethyl cellulose 50 weight portion, Vitamin C
Acid 10 weight portions, obtain uniform mixture after mixing 0.5～1h；
Step 2, the mixture of previous step is proceeded to low-temperature airflow pulverizer, pulverize temperature and control below 20 DEG C, pulverize
Rear mistake 300 mesh sieve, i.e. prepares dispersibility and the good wettable powder of suspension.
Suspensibility is measured according to 4.1 in agricultural-chemical suspension rate assay method GB/T 14825-2006, and wetting time is pressed
Being measured according to pesticide wettable wettability determination method GB/T 5451-2001, fineness is according to pesticide powder, wettability
2.2 wet screenings in powder fineness assay method GB/T 16150-1995 are measured, and moisture is according to pesticide moisture assay method
In GB/T 1600-2001,2.2 azeotropic distillation methods are measured, and pH value is according to the assay method GB/T 1601-of pesticide pH value
1993 are measured.The suspensibility of this wettable powder is 80% after testing, and wetting time is 75s, and fineness 99% is by 48 μm
Sieve, moisture 6.1%, pH6.5.
Embodiment 4
The field experiment of wettable powder preventing and treating graw mold of tomato:
Changping County, Beijing Bai Shan on April 2 town lion battalion village's day-light greenhouse in 2015, experimental field front stubble plantation Fructus Lycopersici esculenti, has
The generation of gray mold.The purpose of this experiment is the generation of preventing and treating graw mold of tomato.By the wettable powder of preparation in embodiment 1,2,3
500 times of liquid of agent carry out foliage-spray and process as experiment, spray 1000 times of liquid of chemical pesticide 50% iprodione wettable powder and make
Positive control, sprays clear water and makees blank, and each process sets 3 repetitions, and the plot area often repeated is 30 square metres, kind
Eggplant Post flowering starts to spray, and sprays every 7 days 1 time, sprays continuously 3 times, is spaced 7 days investigation incidences after spraying for last 1 time.
Carry out field management by producing Routine Management mode, carry out mark in the premorbid method being red rope of the plant to investigating
Note, the strain of every repeated survey 20, investigate graw mold of tomato disease index, total number of sheets of record Fructus Lycopersici esculenti, the sick number of sheets at different levels, calculate the state of an illness
Index and prevention effect.Record each cell production after fruit picking every time, gather in the crops latter stage Fructus Lycopersici esculenti, calculate each community total output and convert into
For per mu yield.
Disease index grade scale: 0 grade, without scab；
1 grade, lesion area accounts for less than the 5% of whole leaf area；
3 grades, lesion area accounts for the 6%-10% of whole leaf area；
5 grades, lesion area accounts for the 11%-25% of whole leaf area；
7 grades, lesion area accounts for the 26%-50% of whole leaf area；
9 grades, lesion area accounts for more than the 50% of whole leaf area.
Disease index (%)=∑ (the sick number of sheets × relative level numerical value at different levels)/(investigating total number of sheets × the highest sick level value) ×
100。
Prevention effect (%)=(blank district disease index-treatment region disease index)/blank district disease index
×100。
Table 1 wettable powder prevention effect on gray mold and the impact on tomato yield
Embodiment 5
The field experiment of wettable powder preventing and treating pepper ralstonia solanacearum:
Cook a meal Guangyang District village village day-light greenhouse in Langfang in Hebei Province on the 22nd March in 2015, the continuous three batches of plantation phases in test plot
With kind Fructus Capsici, there is bacterial wilt to occur, there is Soil-sickness Problem.
The purpose of this experiment is the generation of preventing and treating pepper ralstonia solanacearum.By the wettable powder 500 of preparation in embodiment 1,2,3
Times liquid carries out pouring root and processes as experiment, and 3% Zhongshengmycin WP 600 times liquid irrigating root makees positive control, fills clear water and makees sky
White comparison, each process sets 3 repetitions, and the plot area often repeated is 30 square metres, in bacterial wilt premorbid medication, every 7
It pouring root 1 time, fills 4 times continuously, is spaced 7 days investigation incidences after last 1 pouring root.Field is carried out by producing Routine Management mode
Between manage, carry out labelling the premorbid plant to investigating by the method being red rope, the strain of every repeated survey 40, investigate Fructus Capsici
Dead strain number, calculates mortality rate and prevention effect, records each cell production after each fruit picking, gathers in the crops latter stage in Fructus Capsici, calculates each
Community total output is also converted into per mu yield.
Table 2 wettable powder prevention effect on pepper ralstonia solanacearum and the impact on yield of hot pepper
From greenhouse test embodiment 4,5 it can be seen that the wettable powder prepared of the present invention is to gray mold fungal disease
It is respectively provided with good prevention effect with bacterial wilt bacterial disease, and plant growing way is more prosperous than the comparison of conventional chemical medicament
Contain, the minimum volume increase of Fructus Lycopersici esculenti 14.36%, the minimum volume increase of Fructus Capsici 11.95% can be made.
Claims (5)
1. the wettable powder that a Gliocladium roseum is compounding with bacillus subtilis, it is characterised in that described wettable powder
It is made up of following raw material by weight: 50-100 part glues broom mould fungal spore powder, 50-100 part bacillus subtilis antibacterial bud
Cryptogam, 800-850 part kieselguhr, 10-20 part dodecylbenzene sodium sulfonate, 10-50 part sodium lignin sulfonate, 10-50 part carboxylic first
Base sodium cellulosate, 5-10 part ascorbic acid are compounding to be processed.
The wettable powder that Gliocladium roseum the most according to claim 1 is compounding with bacillus subtilis, it is characterised in that
Described fungal spore powder is to have fungal diseases such as preventing and treating gray mold, banded sclerotial blight, root rot, fungal leaf spot, sclerotiniose
The mould spore powder of viscous broom, purity be more than 90%, water content be less than 10%, chlamydospore ratio be more than 30%.
The wettable powder that Gliocladium roseum the most according to claim 1 is compounding with bacillus subtilis, it is characterised in that
Described bacterial spore powder is to have antibacterials such as preventing and treating bacterial wilt, soft rot, Peptic Ulcers, bacterial speck, bacterial angular leaf spot
The Bacillus globigii spores powder of sexually transmitted disease (STD) evil, purity is more than 90%, and water content is less than 10%.
The wettable powder that Gliocladium roseum the most according to claim 1 is compounding with bacillus subtilis, it is characterised in that
The described mould spore powder of viscous broom is viscous broom mould QGSS-C16 spore powder, and preserving number is CGMCC No.12243.
Gliocladium roseum the most according to claim 1 and bacillus subtilis built wettable powder, it is characterised in that institute
Stating bacillus subtilis spore powder is bacillus subtilis QGSS-05 spore powder, and preserving number is CGMCC No.11563.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610331376.XA CN106305792B (en) | 2016-05-19 | 2016-05-19 | A kind of wettable powder that Gliocladium roseum is compounded with bacillus subtilis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610331376.XA CN106305792B (en) | 2016-05-19 | 2016-05-19 | A kind of wettable powder that Gliocladium roseum is compounded with bacillus subtilis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106305792A true CN106305792A (en) | 2017-01-11 |
| CN106305792B CN106305792B (en) | 2018-12-25 |
Family
ID=57726246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610331376.XA Active CN106305792B (en) | 2016-05-19 | 2016-05-19 | A kind of wettable powder that Gliocladium roseum is compounded with bacillus subtilis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106305792B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129242A (en) * | 2019-06-13 | 2019-08-16 | 北京市农林科学院 | A kind of compound microbial preparation against heavy cropping and preparation method thereof |
| WO2019222819A1 (en) * | 2018-05-23 | 2019-11-28 | Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda | Composition for biological control of phytopathogenic fungi |
| CN110558336A (en) * | 2019-07-19 | 2019-12-13 | 湖南科技学院 | A biocontrol agent for preventing and treating lettuce sclerotinia and its preparation and use method |
| CN111471598A (en) * | 2020-04-25 | 2020-07-31 | 甘肃省科学院生物研究所 | Application of Compound Bacterial Agent and Pest Control of S. |
| CN114262248A (en) * | 2022-01-13 | 2022-04-01 | 宁波市农业科学研究院 | A kind of biological bacterial fertilizer for preventing and treating soft rot of Chinese cabbage and preparation method thereof |
| CN115029286A (en) * | 2022-07-21 | 2022-09-09 | 东北农业大学 | Biocontrol bacterium compound microbial inoculum and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102533608A (en) * | 2012-01-19 | 2012-07-04 | 高丙利 | Bacillus subtilis, preparation, preparation method of preparation, and application of bacillus subtilis and preparation |
| CN102550607A (en) * | 2011-11-01 | 2012-07-11 | 江苏省农业科学院 | Bacillus subtilis PTS-394 for preventing and controlling soil-borne diseases of solanaceous fruit vegetables and application thereof |
| CN103224902A (en) * | 2013-05-08 | 2013-07-31 | 华中农业大学 | Biocontrol bacterium Bacillus subtilis sbz-106, and wettable pulvis and application thereof |
| CN104531533A (en) * | 2014-06-20 | 2015-04-22 | 中农绿康（北京）生物技术有限公司 | Composite microbial inoculant for controlling plant continuous-cropping diseases and preparation method and application thereof |
| CN105072915A (en) * | 2013-03-20 | 2015-11-18 | 巴斯夫公司 | Synergistic composition comprising a bacillus subtilis strain and a biopesticide |
| US20160007613A1 (en) * | 2014-07-14 | 2016-01-14 | Adjuvants Plus Usa, Inc. | Clonostachys rosea Inoculated Plant Materials with Fungicides and Adjuvants |
-
2016
- 2016-05-19 CN CN201610331376.XA patent/CN106305792B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102550607A (en) * | 2011-11-01 | 2012-07-11 | 江苏省农业科学院 | Bacillus subtilis PTS-394 for preventing and controlling soil-borne diseases of solanaceous fruit vegetables and application thereof |
| CN102533608A (en) * | 2012-01-19 | 2012-07-04 | 高丙利 | Bacillus subtilis, preparation, preparation method of preparation, and application of bacillus subtilis and preparation |
| CN105072915A (en) * | 2013-03-20 | 2015-11-18 | 巴斯夫公司 | Synergistic composition comprising a bacillus subtilis strain and a biopesticide |
| CN103224902A (en) * | 2013-05-08 | 2013-07-31 | 华中农业大学 | Biocontrol bacterium Bacillus subtilis sbz-106, and wettable pulvis and application thereof |
| CN104531533A (en) * | 2014-06-20 | 2015-04-22 | 中农绿康（北京）生物技术有限公司 | Composite microbial inoculant for controlling plant continuous-cropping diseases and preparation method and application thereof |
| US20160007613A1 (en) * | 2014-07-14 | 2016-01-14 | Adjuvants Plus Usa, Inc. | Clonostachys rosea Inoculated Plant Materials with Fungicides and Adjuvants |
Non-Patent Citations (3)
| Title |
|---|
| 吴孔明: "《植保科技创新与病虫防控专业化》", 30 November 2011, 中国农业科学技术出版社 * |
| 巩亭云: ""1.多巴丝肼口腔崩解片的药学研究 2.粘帚霉孢子可湿性粉剂的研究"", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
| 徐映明: "《新世纪农药新品种应用手册》", 31 January 2016, 中国农业科学技术出版社 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019222819A1 (en) * | 2018-05-23 | 2019-11-28 | Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda | Composition for biological control of phytopathogenic fungi |
| CN110129242A (en) * | 2019-06-13 | 2019-08-16 | 北京市农林科学院 | A kind of compound microbial preparation against heavy cropping and preparation method thereof |
| CN110129242B (en) * | 2019-06-13 | 2021-07-13 | 北京市农林科学院 | A kind of composite microbial preparation resistant to continuous cropping and preparation method thereof |
| CN110558336A (en) * | 2019-07-19 | 2019-12-13 | 湖南科技学院 | A biocontrol agent for preventing and treating lettuce sclerotinia and its preparation and use method |
| CN111471598A (en) * | 2020-04-25 | 2020-07-31 | 甘肃省科学院生物研究所 | Application of Compound Bacterial Agent and Pest Control of S. |
| CN114262248A (en) * | 2022-01-13 | 2022-04-01 | 宁波市农业科学研究院 | A kind of biological bacterial fertilizer for preventing and treating soft rot of Chinese cabbage and preparation method thereof |
| CN115029286A (en) * | 2022-07-21 | 2022-09-09 | 东北农业大学 | Biocontrol bacterium compound microbial inoculum and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106305792B (en) | 2018-12-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106305792A (en) | Clonostachys rosea-bacillus subtilis composite wettable powder | |
| CN102787075B (en) | Trichoderma viride strain and application thereof | |
| CN103184184B (en) | Streptomyces albidoflavus and applications thereof | |
| CN104761379A (en) | Novel multifunctional compound microbial fertilizer and preparation method thereof | |
| CN103342604A (en) | Bio-control bacterium foliage fertilizer, and preparation method and application thereof | |
| CN105316249B (en) | A kind of bacillus subtilis and microbial bacterial agent and their applications in fermentation maturity | |
| Zhao et al. | Effects of Bacillus subtilis NCD-2 and broccoli residues return on potato Verticillium wilt and soil fungal community structure | |
| CN105165824A (en) | Solid carrier or carrier liquid for producing agricultural microbial preparation | |
| CN104496635A (en) | Sweet potato special-purpose fertilizer | |
| CN106190929B (en) | A kind of new strain of Bacillus licheniformis and its application | |
| CN105016904A (en) | Special biological fertilizer for preventing and controlling peanut nematodosis and preparation method thereof | |
| CN104774772A (en) | Straw decomposing fungicide with fertility increasing effect and application of fungicide | |
| CN112029667B (en) | Trichoderma, trichoderma spore suspension, trichoderma zymophyte powder and preparation method and application thereof | |
| CN102925366B (en) | A Strain of Trichoderma viride and Its Application on Cucumber | |
| CN108148765A (en) | One plant of acidproof plan trichodermaharzianum and its application on Rhizoctonia solani Kuhn is inhibited | |
| CN106978374A (en) | A kind of microbial soil conditioner and preparation method thereof | |
| CN106396991A (en) | Compound microbial fertilizer capable of resisting nematode and promoting crop growth | |
| CN112574906B (en) | Bacterial strain for preventing and treating common continuous cropping diseases of greenhouse tomatoes and compound microbial agent thereof | |
| CN108658679A (en) | It is a kind of to be used to prevent organic fertilizer of early blight of tomato and preparation method thereof | |
| CN102167641A (en) | 200 million live spore/g lichen Bacillus subtilis medical fertilizer granule and preparation method thereof | |
| CN108496995A (en) | A kind of biological seed dressing liquor and its preparation method and application | |
| CN106804627A (en) | A kind of complex microorganism Wettable pesticide powder and preparation method thereof and purposes | |
| CN103045482A (en) | Trichoderma harzianum strain | |
| CN106376600A (en) | Paecilomyces fumosoroseus wettable powder and preparation method thereof | |
| CN102648713A (en) | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 100193, room 3, building 3009, building 3, China Agriculture University international pioneer park, 10 Tian Xiu Road, Beijing, Haidian District Applicant after: Beijing hi tech biological technology Co., Ltd. Address before: 100081, room 209, white building, No. 7 South Street, Haidian District, Beijing, Zhongguancun Applicant before: Beijing hi tech biological technology Co., Ltd. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |