CN106266408A - A kind of use Huangqi Compound medicine repairing nerve damage Therapeutic Method - Google Patents

Abstract

The invention provides a kind of use Huangqi Compound medicine repairing nerve damage Therapeutic Method, Huangqi Compound medicine is prepared from by the raw material of following weight portion: the Radix Astragali 50 70 parts, Radix Codonopsis 3 10 parts, 3 10 parts of Flos Carthami, Rhizoma Chuanxiong 3 10 parts, 2 10 parts of Poria, the Radix Angelicae Dahuricae 10 15 parts, Rhizoma Et Radix Notopterygii 15 parts, Cortex Magnoliae Officinalis 15 parts, the Rhizoma Atractylodis Macrocephalae 2 11 parts, Radix Rehmanniae 18 parts, Radix Bupleuri 16 parts, Rhizoma Cyperi 23 parts, Semen Raphani 18 parts, Radix Scutellariae 26 parts.Subject treatment method has the CO2 laser weld promoting damage, improves neuranagenesis quality characteristic, and prescription compatibility is reasonable, and methodological science determined curative effect is suitable in field of medicaments large-scale promotion.

Description

A treatment method for repairing nerve damage using Astragalus compound medicine

technical field

The invention belongs to the technical field of medical treatment, and in particular relates to a treatment method for repairing nerve damage by using astragali compound medicine.

Background technique

After peripheral nerve injury, the nerve undergoes Wallerian degeneration below the level of injury, and then regenerates at a rate of 1mm per day. How to promote nerve regeneration and improve the quality of nerve regeneration has always been a difficult problem for the medical community to solve. After nerve injury, active and effective treatment is an important condition for the effective recovery of nerve function. For nerve fibers that remain intact and nerve disuse injuries, the main treatment is drug therapy; for injuries caused by complete or partial nerve rupture and nerve entrapment, within a long period of time after nerve suture or release It needs to cooperate with drug treatment to create a good internal environment for nerve repair. Drugs commonly used in clinical practice at this stage include vitamin B complex, nerve growth factor, ganglioside and so on. Different from the single action mechanism of other drugs, traditional Chinese medicine is a multi-factor complex, which has unique advantages because it may provide a richer environment with a ratio closer to neurophysiological needs and growth active factors.

Therefore it is very necessary to invent a kind of novel traditional Chinese medicine compound medicine to be used for repairing nerve injury.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a treatment method for repairing nerve damage by using the astragalus compound drug, which can effectively and rapidly repair nerve damage.

In order to achieve the above object, the present invention is achieved through the following technical solutions: the present invention provides a method for repairing nerve damage using the astragalus compound medicine, which is prepared from the following raw materials in parts by weight: 50-70 parts of Astragalus, 3-10 parts of Codonopsis 3-10 parts of safflower, 3-10 parts of Chuanxiong, 2-10 parts of Poria cocos, 10-15 parts of Angelica dahurica, 1-5 parts of notopterygium, 1-5 parts of Magnolia officinalis, 2-11 parts of Atractylodes macrocephala, 1-1 8 parts, 1-6 parts of Bupleurum, 2-3 parts of Rhizoma Cyperi, 1-8 parts of Radish Seed, 2-6 parts of Scutellaria baicalensis;

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Weigh Cyperus cyperi according to the weight of the prescription, pass through No. 2 to No. 3 sieves after crushing, put the powder in a volatile oil extractor, add an appropriate amount of water, soak for 5-6 hours, and continuously reflux for 9-12 hours. collect volatile oil;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix to obtain the total powder, add an appropriate amount of ethanol solution, heat and reflux for extraction for 5-8 hours, cool, filter and remove the filter residue to obtain the filtrate, and recover the ethanol from the filtrate to concentrate Extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Preferably, the Astragalus compound medicine is prepared from the following raw materials in parts by weight: 55-68 parts of Astragalus, 4-9 parts of Rhizoma Chuanxiong, 4-10 parts of Codonopsis Codonopsis, 3-10 parts of Safflower, 3-8 parts of Poria cocos, 10-15 parts of Angelica dahurica, 2-4 parts of notopterygium, 2-4 parts of Magnolia officinalis, 4-10 parts of Atractylodes macrocephala, 2-6 parts of rehmannia root, 2-5 parts of Bupleurum, 2-3 parts of Rhizoma Cyperi, 2-3 parts of radish 7 parts, 2-6 parts of Radix Scutellariae.

Preferably, the Astragalus compound drug is prepared from the following raw materials in parts by weight: 55 parts of Astragalus, 4 parts of Codonopsis, 4 parts of Safflower, 5 parts of Rhizoma Chuanxiong, 4 parts of Poria cocos, 5 parts of Angelica dahurica, 3 parts of Notopterygium, Magnolia officinalis 2 parts, 6 parts of Atractylodes macrocephala, 3 parts of Rehmannia glutinosa, 2 parts of Bupleurum, 3 parts of Cyperus cyperi, 3 parts of radish seed, and 3 parts of Scutellaria baicalensis.

Preferably, the Astragalus compound drug is prepared from the following raw materials in parts by weight: 68 parts of Astragalus membranaceus, 5 parts of Codonopsis pilosula, 3 parts of safflower, 6 parts of Rhizoma Chuanxiong, 5 parts of Poria cocos, 6 parts of Angelica dahurica, 2 parts of notopterygium, Magnolia officinalis 2 parts, 7 parts of Atractylodes macrocephala, 1 part of rehmannia root, 3 parts of Bupleurum, 2 parts of Cyperus cyperi, 3 parts of radish seed, and 3 parts of skullcap.

Preferably, the relative concentration of the ethanol solution in step (3) of the preparation method is 70%.

Preferably, the uniform stirring time in step (4) of the preparation method is 30-50 min.

The effect of each raw material among the present invention is as follows:

Astragalus: Astragalus has the effects of invigorating qi and solidifying the surface, diuretic and detoxification, draining pus, astringing sores and promoting granulation. For qi deficiency and fatigue, lack of appetite, loose stools, subsidence of central qi, chronic diarrhea and prolapse of the anus, blood in the stool and metrorrhagia, spontaneous perspiration due to superficial deficiency, carbuncle and gangrene that are difficult to ulcerate, chronic ulceration without restraint, blood deficiency and chlorosis, internal heat and quenching thirst. "The Classic" records: "mainly carbuncle and gangrene, long-term sores, draining pus and relieving pain. Tonifies deficiency and treats all kinds of diseases in children." "Hanako Materia Medica" records: "Helps Qi and strengthens muscles and bones, grows flesh and nourishes blood.

Codonopsis: It has the effects of invigorating the middle and replenishing qi, invigorating the spleen and benefiting the lungs. Codonopsis pilosula has the effects of enhancing immunity, dilating blood vessels, lowering blood pressure, improving microcirculation, and enhancing hematopoietic function. In addition, it can improve the leukopenia caused by chemotherapy and radiotherapy.

Safflower: often used with peach kernel, angelica, chuanxiong, rehmannia glutinosa, red peony, etc., called Taohong Siwu Decoction. For coronary heart disease, it can be used with Chuanxiong and Danshen. For bruises, swelling and pain due to blood stasis, costal pain due to blood stasis, carbuncle and hematemesis with stasis, it can be used with peach kernels, frankincense, and myrrh.

Ligusticum Chuanxiong: This product is pungent and warm, can promote blood circulation and remove blood stasis, and can promote qi and relieve pain. It is a "qi medicine in blood" and has the effect of promoting qi and blood.

Poria cocos: sweet in taste, light in taste, flat in nature. Guixin, lung, spleen, kidney channel. Inducing diuresis to eliminate dampness, invigorating the spleen, calming the mind. Cure mainly for edema, oliguria, phlegm retention, dizziness and palpitation, deficiency of the spleen, lack of food, loose stool and diarrhea, restlessness, palpitation and insomnia.

Angelica dahurica: nature and flavor are pungent, warm. Return through entering lung, spleen, stomach meridian. Function cures mainly expelling wind, drying damp, detumescence, pain relieving. Cure headache, brow bone pain, toothache, nasal sinusitis, cold-damp abdominal pain, intestinal wind and hemorrhoids, red and white vaginal discharge, carbuncle sores, dry itchy skin, and scabies.

Notopterygium: expelling exterior cold; expelling rheumatism; sharpening joints; relieving pain. Main exogenous wind-cold; headache without sweating; mimeograph cold-damp arthralgia; Fengshui edema; sore swollen toxin. For impotence and nocturnal emission, enuresis and frequent urination, cold pain in the waist and knees, asthma due to kidney deficiency, diarrhea at dawn; external use for treating vitiligo and alopecia areata. Relieve the exterior and dispel cold, expelling wind and dampness, relieving pain.

Magnolia officinalis: pungent in taste, warm in nature; promotes qi and eliminates stagnation, dries dampness and relieves fullness, subdues adverse events and relieves asthma, mainly treats food stagnation and stagnation of qi, abdominal distension and constipation, dampness resistance in the middle burner, nausea and vomiting, phlegm stagnation and qi reversal, chest fullness and coughing .

Atractylodes macrocephala: use the rhizome as medicine. Dry dampness and invigorate the spleen, insufficiency of the spleen, lack of food, indigestion, chronic diarrhea, edema, antiperspirant, and antifertility.

Rehmannia glutinosa: Fresh rehmannia glutinosa clears away heat and promotes body fluid, and stops bleeding after cooling; it is used for hot wind hurting yin, crimson tongue and polydipsia, spots and rashes, hematemesis, epistaxis, and sore throat. Radix Rehmanniae clears away heat and cools blood, nourishes yin, and promotes body fluid; it is used for fever, polydipsia, spot rash, internal heat due to yin deficiency, hematemesis, epistaxis, diabetes, and infectious hepatitis. .

Bupleurum: bitter in nature and flavor, slightly cold, returns to the liver and gallbladder meridians. It has the effects of reconciling the exterior and interior, soothing the liver and promoting yang. For cold and fever, alternating cold and heat, malaria, liver depression and Qi stagnation, sternocostal pain, anal prolapse, uterine prolapse, and irregular menstruation.

Rhizoma Cyperae: bitter, cold. Return lung, liver, heart meridian; detoxify and reduce swelling: used for sore throat, sore swelling and pain, etc.; relieving cough and reducing phlegm: used for cough with excessive phlegm, cough with lung heat, thick yellow phlegm, etc.

Radix Seed: Radix Seed has the effects of eliminating food, regulating qi, reducing phlegm, relieving cough, clearing lung and relieving throat, dissipating blood stasis and reducing swelling. Indications for stagnation of Qi due to food accumulation, fullness in the abdomen, acid regurgitation, hiccups, diarrhea, dysentery, sore throat, coughing up phlegm, hoarseness, breast swelling and pain in women, milk blockage, external treatment injury and bruising embolism.

Scutellaria baicalensis: the root is used as medicine to clear away heat and dampness, cool blood and induce miscarriage, and detoxify. Indications for febrile disease, upper respiratory tract infection, lung-heat cough, damp-heat jaundice, pneumonia, dysentery, hemoptysis, red eyes, restless fetus, high blood pressure, carbuncle, swollen boils and other diseases.

Beneficial effects of the present invention:

The Radix Astragali compound drug used in the present invention is scientifically compatible with various raw materials to achieve the same effect. The preparation method of the therapeutic drug is simple and practical. In combination with the treatment method, the drug is used to treat the disease body by gavage, which can promote the repair of damaged nerves. The characteristics of improving the quality of nerve regeneration, the composition of the prescription is reasonable, the method is scientific, the curative effect is definite, and it is suitable for large-scale promotion in the field of medicine.

detailed description

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

Example 1:

The invention discloses a method for treating nerve damage by using the Astragalus compound medicine. The Astragalus compound medicine is prepared from the following raw materials in parts by weight: 50 parts of Astragalus membranaceus, 4 parts of Codonopsis pilosula, 3 parts of safflower, 3 parts of Rhizoma Chuanxiong, 2 parts of Poria cocos, 5 parts of Angelica dahurica, Notopterygium 1 part, Magnolia officinalis 1 part, Atractylodes macrocephala 2 parts, Rehmannia glutinosa 1 part, Bupleurum 1 part, Cyperus cyperi 2 parts, radish seed 1 part, Scutellaria baicalensis 2 parts.

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Cyperi cyperi was weighed in parts by weight of the prescription, crushed and passed through No. 2 to No. 3 sieves, the powder was placed in a volatile oil extractor, an appropriate amount of water was added, soaked for 5 hours, continuously refluxed for 9 hours, and the volatile oil was collected;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix them to obtain a total powder, add an ethanol solution with a relative concentration of 70%, heat and reflux for extraction for 5 hours, cool, filter and remove the filter residue to obtain a filtrate, and recover the filtrate Ethanol to obtain concentrated extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly for 30 minutes, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Example 2:

The invention discloses a method for treating nerve damage by using compound Astragalus medicaments. The compound Astragalus medicaments are prepared from the following raw materials in parts by weight: 70 parts of Astragalus membranaceus, 8 parts of Codonopsis pilosula, 3 parts of safflower, 4 parts of Rhizoma Chuanxiong, 3 parts of Poria cocos, 5 parts of Angelica dahurica, Notopterygium 2 parts, Magnolia officinalis 2 parts, Atractylodes macrocephala 4 parts, Rehmannia glutinosa 2 parts, Bupleurum 2 parts, Cyperus cyperi 2 parts, Laifuzi 2 parts, Scutellaria baicalensis 6 parts.

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Cyperi cyperi was weighed in parts by weight of the prescription, crushed and passed through No. 2 to No. 3 sieves, the powder was placed in a volatile oil extractor, an appropriate amount of water was added, soaked for 6 hours, continuously refluxed for 12 hours, and the volatile oil was collected;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix to obtain the total powder, add ethanol solution with a relative concentration of 70%, heat and reflux for extraction for 8 hours, cool, filter and remove the filter residue to obtain the filtrate, and recover the filtrate Ethanol to obtain concentrated extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly for 50 minutes, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Example 3:

The invention discloses a method for treating nerve damage by using the Astragalus compound medicine. The Astragalus compound medicine is prepared from the following raw materials in parts by weight: 55 parts of Astragalus membranaceus, 6 parts of Codonopsis pilosula, 8 parts of safflower, 4 parts of Rhizoma Chuanxiong, 3 parts of Poria cocos, 5 parts of Angelica dahurica, Notopterygium 2 parts, Magnolia officinalis 2 parts, Atractylodes macrocephala 4 parts, Rehmannia glutinosa 2 parts, Bupleurum 2 parts, Cyperus cyperi 2 parts, Laifuzi 2 parts, Scutellaria baicalensis 2 parts.

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Cyperi cyperi was weighed in parts by weight of the prescription, crushed and passed through No. 2 to No. 3 sieves, the powder was placed in a volatile oil extractor, an appropriate amount of water was added, soaked for 6 hours, continuously refluxed for 10 hours, and the volatile oil was collected;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix them to obtain the total powder, add an ethanol solution with a relative concentration of 70%, heat and reflux for extraction for 6 hours, cool, filter and remove the filter residue to obtain a filtrate, and recover the filtrate Ethanol to obtain concentrated extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly for 40 minutes, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Example 4:

The invention discloses a method for treating nerve damage by using a compound medicine of Astragalus membranaceus. The compound medicine of Astragalus membranaceus is prepared from the following raw materials in parts by weight: 68 parts of Astragalus membranaceus, 5 parts of Codonopsis pilosula, 6 parts of safflower, 5 parts of Chuanxiong, 4 parts of Poria cocos, 5 parts of Angelica dahurica, Notopterygium 3 parts, Magnolia officinalis 2 parts, Atractylodes macrocephala 6 parts, Rehmannia glutinosa 3 parts, Bupleurum 2 parts, Cyperus cyperi 3 parts, Laifuzi 3 parts, Scutellaria baicalensis 3 parts.

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Cyperi cyperi was weighed in parts by weight of the prescription, crushed and passed through No. 2 to No. 3 sieves, the powder was placed in a volatile oil extractor, an appropriate amount of water was added, soaked for 5 hours, and continuously refluxed for 10 hours to collect the volatile oil;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix them to obtain the total powder, add an ethanol solution with a relative concentration of 70%, heat and reflux for extraction for 6 hours, cool, filter and remove the filter residue to obtain a filtrate, and recover the filtrate Ethanol to obtain concentrated extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly for 40 minutes, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Example 5:

The invention discloses a method for treating nerve damage by using compound Astragali medicaments. The compound Astragalus medicaments are prepared from the following raw materials in parts by weight: 65 parts of Astragalus membranaceus, 10 parts of Codonopsis pilosula, 4 parts of safflower, 6 parts of Chuanxiong, 5 parts of Poria cocos, 6 parts of Angelica dahurica, 2 parts of notopterygium, 2 parts of Magnolia officinalis, 7 parts of Atractylodes macrocephala, 1 part of rehmannia root, 3 parts of Bupleurum, 2 parts of Cyperus cyperi, 3 parts of radish seed, and 3 parts of skullcap.

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Cyperi cyperi was weighed in parts by weight of the prescription, crushed and passed through No. 2 to No. 3 sieves, the powder was placed in a volatile oil extractor, an appropriate amount of water was added, soaked for 5 hours, and continuously refluxed for 10 hours to collect the volatile oil;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix them to obtain the total powder, add an ethanol solution with a relative concentration of 70%, heat and reflux for extraction for 6 hours, cool, filter and remove the filter residue to obtain a filtrate, and recover the filtrate Ethanol to obtain concentrated extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly for 40 minutes, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Embodiment 6:

The invention discloses a method for treating nerve damage by using a compound medicine of Astragalus membranaceus. The compound medicine of Astragalus membranaceus is prepared from the following raw materials in parts by weight: 65 parts of Astragalus membranaceus, 4 parts of Codonopsis pilosula, 3 parts of safflower, 3 parts of Rhizoma Chuanxiong, 2 parts of Poria cocos, 5 parts of Angelica dahurica, Notopterygium 1 part, Magnolia officinalis 1 part, Atractylodes macrocephala 2 parts, Rehmannia glutinosa 1 part, Bupleurum 1 part, Cyperus cyperi 2 parts, radish seed 1 part, Scutellaria baicalensis 6 parts.

Its treatment method is:

(1) firstly take Poria cocos and raisin seed and mix by weight parts of the prescription, be ground into very fine powder (all pass through No. 9 sieve 200 meshes), get mixed powder A;

(2) Cyperi cyperi was weighed in parts by weight of the prescription, crushed and passed through No. 2 to No. 3 sieves, the powder was placed in a volatile oil extractor, an appropriate amount of water was added, soaked for 6 hours, continuously refluxed for 10 hours, and the volatile oil was collected;

(3) Weigh the remaining 11 medicinal materials according to the weight of the prescription, grind them separately, mix to obtain the total powder, add ethanol solution with a relative concentration of 70%, heat and reflux for extraction for 8 hours, cool, filter and remove the filter residue to obtain the filtrate, and recover the filtrate Ethanol to obtain concentrated extract B;

(4) Finally, add Cyperus cyperi volatile oil into the concentrated extract B and stir evenly for 50 minutes, then add the mixed powder A into the concentrated extract B, and stir well to obtain the mixed extract C (concentration 2g/ml);

(5) Take the extract prepared in step (4) orally, twice a day, once at 8:00-8:30 in the morning, and once at 4:00-4:30 in the afternoon, for 14 consecutive days .

Curative effect observation:

Methods: 60 female Wistar rats were established with bilateral sciatic nerve clamp injury (hemostatic forceps closing the second tooth) model, and randomly divided into high-dose group (group A), middle-dose group (group B) and low-dose group (Group C) and blank control group (Group D). Respectively intraperitoneal 2ml/d, 1ml/d, 0.5ml/d drug and 2ml/d saline. Continuous administration for 28 days. At 4, 6, and 8 weeks after operation, the general observation was performed, the electroneurogram of the regenerated nerve was measured, histological and transmission electron microscope observations were performed, the morphological changes of the target organs were analyzed, and the regeneration quality and functional recovery of the sciatic nerve were evaluated by immunohistochemical staining and other methods .

Results: (1) Nerve electrophysiology: The motor nerve conduction velocity and action potential amplitude of the regenerated nerve at each time point after 4, 6, and 8 weeks increased with time, and the values of the two indicators at each time point were generally A in the longitudinal comparison. >B>C>D, but there was no significant difference between group A and group B, group C and group D (P>0.05); group A, group B and group D, group B and group C The difference was significant (P<0.05). (2) Observation of nerve HE staining at 8 weeks after operation: It can be seen that the regenerated nerves in each group grew across the anastomosis to the distal end, and inflammatory cells infiltrated around the sutured eyes to varying degrees. The myelinated nerve fibers in group A were uniform in thickness and arranged neatly, the axonal myelin sheath was relatively complete, and the nerve regeneration was more mature; the myelinated nerve fibers in group B were slightly thinner and arranged loosely. Most of the nerve fibers in group C were myelinated nerve fibers, the nerve fibers were slightly thinner, and the arrangement was slightly loose. In group D, the nerve fibers were loosely arranged, and some nerve fibers had no axons and myelin sheaths. Nerve methylene blue-basic fuchsin staining shows that the average diameter of myelinated nerve fibers and myelin sheath thickness in groups A and B are relatively uniform, the shape of myelin sheath is regular, and there is less interfascicular connective tissue; the average diameter of myelinated nerve fibers and myelin sheath in groups C and D The thickness is different, the shape of myelin sheath is irregular, and the hyperplasia of connective tissue is obvious (×40).

(3) Observation by transmission electron microscope at 8 weeks after operation: the number of regenerated nerve fibers in groups A, B, and C is large, the nerve fibers are thick, the ratio of axon to myelin sheath is basically 1:1, the myelin sheath is thicker, the lamellar layer is dense, and the light and dark plate Clear, relatively uniform structural maturity. The diameter of the regenerated nerve fibers in group D was quite different, the myelin sheath was thin, the shape was irregular, the distortion was obvious, the lamellar layer was loose, the maturity was not good, and new unmyelinated nerve fibers were scattered among them.

(4) HE staining observation of triceps calf muscle at 12 weeks after operation: the muscle cells in groups A and B were neatly arranged, relatively compact, with large cross-sectional area and thick muscle fibers, and the gap between muscle fibers in group B was wider than that in group A. In group C, the muscle fibers are of different thickness, the gap between muscle fibers is wide, and there is hyperplasia of connective tissue; in group D, the atrophic muscle fibers are obviously horn-shaped, distributed in small clusters in the muscle bundle, with more deeply stained nuclei, and the cross-sectional area of muscle fibers is small , the gap between muscle fibers widened, more connective tissue hyperplasia. The triceps calf muscle TSCI and cross-sectional area of muscle fibers in each group were compared in pairs, group A, group B were compared with group D, and group B was significantly different from group C (P<0.05); There was no significant difference between group C and group D (P>0.05).

(5) Immunohistochemical staining: 4 weeks after operation, there was a significant difference in the expression of NGF protein in the regenerated nerve between each group (P<0.05); Compared with group B, there was a significant difference between group C (P<0.05); compared with group A, there was no significant difference between group C and group D (P>0.05).

Through curative effect observation, it can be seen that the present invention uses Astragalus compound medicine to repair nerve damage treatment method, which has good effect and quick effect, and the rats are rechecked and counted two months after gavage, and there is no infection, and the normal gait movement is quickly restored, and there is no adverse reaction. .

The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be described in the foregoing embodiments Modifications are made to the recorded technical solutions, or equivalent replacements are made to some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (6)

1. one kind uses Huangqi Compound medicine repairing nerve damage Therapeutic Method, it is characterised in that described Huangqi Compound medicine It is prepared from by the raw material of following weight portion: Radix Astragali 50-70 part, Radix Codonopsis 3-10 part, Flos Carthami 3-10 part, Rhizoma Chuanxiong 3-10 part, Poria 2-10 part, Radix Angelicae Dahuricae 10-15 part, Rhizoma Et Radix Notopterygii 1-5 part, Cortex Magnoliae Officinalis 1-5 part, Rhizoma Atractylodis Macrocephalae 2-11 part, Radix Rehmanniae 1-8 part, Radix Bupleuri 1-6 part, Rhizoma Cyperi 2-3 part, Semen Raphani 1-8 part, Radix Scutellariae 2-6 part；

Its Therapeutic Method is:

(1) first weigh Poria by prescription parts by weight to mix with Semen Raphani, be ground into impalpable powder (all by No. 9 sieves 200 Mesh), obtain mixed-powder A；

(2) weigh Rhizoma Cyperi by prescription parts by weight, cross No. 2 to No. 3 sieves after pulverizing, this powder is put in volatile oil extractor, adds Enter suitable quantity of water, soak 5-6 hour, continuous circumfluence extraction 9-12 hour, collect volatile oil；

(3) weigh remaining 11 taste medical material by prescription parts by weight, individually pulverize, mix to obtain total powder, add ethanol in proper amount molten Liquid, heating and refluxing extraction 5-8 hour, cooling, filter removal filtering residue and obtain filtrate, filtrate recycling ethanol obtains concentrated extract B；

(4) finally Rhizoma Cyperi volatile oil is added in concentrated extract B and stirs, then mixed-powder A is added in concentrated extract B, Stir, extractum C (concentration 2g/ml) must be mixed；

(5) extractum step (4) produced uses oral way to take, one day twice, morning 8:00-8:30 gavage once, under Noon 4:00-4:30 gavage once, takes 14 days continuously.

2. use Huangqi Compound medicine repairing nerve damage Therapeutic Method as claimed in claim 1, it is characterised in that described Huangqi Compound medicine is prepared from by the raw material of following weight portion: Radix Astragali 55-68 part, Radix Codonopsis 4-8 part, Flos Carthami 3-10 part, Rhizoma Chuanxiong 4-9 part, Poria 3-8 part, Radix Angelicae Dahuricae 10-15 part, Rhizoma Et Radix Notopterygii 2-4 part, Cortex Magnoliae Officinalis 2-4 part, Rhizoma Atractylodis Macrocephalae 4-10 part, Radix Rehmanniae 2-6 part, Radix Bupleuri 2- 5 parts, Rhizoma Cyperi 2-3 part, Semen Raphani 2-7 part, Radix Scutellariae 2-6 part.

3. use Huangqi Compound medicine repairing nerve damage Therapeutic Method as claimed in claim 2, it is characterised in that described Huangqi Compound medicine is prepared from by the raw material of following weight portion: the Radix Astragali 55 parts, Radix Codonopsis 4 parts, 4 parts of Flos Carthami, Rhizoma Chuanxiong 5 parts, Poria 4 Part, the Radix Angelicae Dahuricae 5 parts, Rhizoma Et Radix Notopterygii 3 parts, Cortex Magnoliae Officinalis 2 parts, the Rhizoma Atractylodis Macrocephalae 6 parts, Radix Rehmanniae 3 parts, Radix Bupleuri 2 parts, Rhizoma Cyperi 3 parts, Semen Raphani 3 parts, Radix Scutellariae 3 Part.

4. use Huangqi Compound medicine repairing nerve damage Therapeutic Method as claimed in claim 2, it is characterised in that described Huangqi Compound medicine is prepared from by the raw material of following weight portion: the Radix Astragali 68 parts, Radix Codonopsis 5 parts, 3 parts of Flos Carthami, Rhizoma Chuanxiong 6 parts, Poria 5 Part, the Radix Angelicae Dahuricae 6 parts, Rhizoma Et Radix Notopterygii 2 parts, Cortex Magnoliae Officinalis 2 parts, the Rhizoma Atractylodis Macrocephalae 7 parts, Radix Rehmanniae 1 part, Radix Bupleuri 3 parts, Rhizoma Cyperi 2 parts, Semen Raphani 3 parts, Radix Scutellariae 3 Part.

5. use Huangqi Compound medicine repairing nerve damage Therapeutic Method as claimed in claim 1, it is characterised in that described In preparation method step (3), the relative concentration of ethanol solution is 70%.

6. use Huangqi Compound medicine repairing nerve damage Therapeutic Method as claimed in claim 1, it is characterised in that described The uniform stirring time in preparation method step (4) is 30-50min.