CN106265814B - Cover the preparation method of application and the drug of watt Ji extract in preparation treatment scald medicament - Google Patents

Abstract

The invention discloses a kind of application for covering watt Ji extract in preparation treatment scald medicament and the preparation methods of the drug.Present invention discover that covering effective component of watt Ji as drug, and it was proved that, covering watt Ji has significant healing effect to scald, and wound is not easy the characteristics of staying scar more afterwards, also has the function of preventing scald wound from infecting, and scald wound can also be accelerated to form a scab, make Wound Contraction and wound healing, compared with existing common medicament for treating scald, there is better therapeutic effect, especially preventing because more having a clear superiority in body fluid exosmose caused by scalding.

Description

Application of imbricate thistle extract in preparation of medicine for treating scald and preparation of the medicine method

technical field

The invention relates to the technical field of pharmacy, in particular to the application of imbricate thistle extract in the preparation of medicine for treating scald and the preparation method of the medicine.

Background technique

Burns and scalds are tissue damages caused by heat, chemicals, light, electricity, and radiation, mainly referring to skin and (or) mucous membrane damage, and severe cases can damage subcutaneous tissue, such as muscles, bones, joints, and even internal organs. , is an extremely complex traumatic disease.

Western medicine then thinks, the cause of disease of burn and scald is because high temperature, as flame, boiling water, molten steel, steam; Chemical factor, as strong acid, strong alkali; Electricity factor, as electric shock, lightning; Directly cause local tissue damage, causing degeneration, necrosis, inflammation, and even structural changes in tissue components, directly leading to destruction of human tissue and loss of function.

Clinically, the degree of burns and scalds is habitually adopted "three degrees and four points" method. According to the degree of damage, it is divided into:

First-degree burns: only the epidermis is injured, and the local skin is red, so it is also called erythema burns. There is mild swelling and pain. Generally, the erythema disappears after 2 to 3 days, and the local necrotic epidermal cells are repaired by deep cell proliferation. Desquamation occurs clinically, leaving no scars, and sometimes mild pigmentation.

Superficial second-degree burns: Injuries to the full-thickness epidermis and superficial dermis, the epidermis and dermis are separated, and exudate accumulates in it to form blisters. Exfoliation of the vesicular epidermis reveals a reddish base with uniform bright red spots, which are sections of congested vascular plexus in the papillary dermis. The skin temperature is high, there is a lot of exudation, the swelling is obvious, and the pain is severe and hyperesthesia due to the stimulation of the peripheral nerves. A thin layer of brownish-yellow dryness will form in 3 to 4 days. If there is no infection, the residual epidermis will heal after 10 to 14 days of hyperplasia, and there will be pigmentation after healing, but no scars.

Deep second-degree burns: The deep layer of the dermis is injured, but there are residual skin appendages. Blisters can also be formed, but because the deteriorated epidermal tissue is slightly thicker, the vesicles are smaller or flatter, and the base is light red or red and white, or reticulated blood vessels can be seen; the sensation is dull, the skin temperature is slightly lower, and the surface is oozing. There is less fluid, but the bottom is swollen obviously. The wound surface gradually dries up 1-2 days after the injury, and can heal within 3-4 weeks if there are no complications such as infection; paralysis marks remain after healing, and if infected, the remaining skin appendages are often destroyed and become grade III.

Third-degree burns: Injury to the whole layer of the skin, even reaching the subcutaneous, muscle, bone, etc., and the skin is necrotic. After dehydration, it can form burns and scalds, so it is also called burns and scalds. The wound surface can be pale, brown or charred, charred, or arborized embolized blood vessels can be seen; the local area becomes hard and dry without blisters. However, there is a large amount of fluid accumulation in the subcutaneous tissue space, and the granulation wound gradually separates and exposes the granulation wound in 2 to 4 weeks after the injury. Except for a small area that can heal itself, it generally needs to be healed through skin transplantation, leaving paralyzed wounds after healing. Scars or deformities, inability to sweat.

Most of the commonly used chemical drugs for the treatment of burns and scalds today are antibiotics such as penicillin, cephalosporin, sulfadiazine, etc., mainly to prevent and treat burn wound infection, anti-inflammatory, and antibacterial therapeutic effects.

Cirsium leducei (Franch.) Levl is a perennial herb of Compositae. Mainly distributed in Guizhou, Guangdong, Guangxi and Yunnan. It grows in forests or forest margins and grasslands on hillsides, at an altitude of 500-1500 meters. Imbricate thistle root has the functions of clearing away heat, detoxification, pain relief, antibacterial and anti-inflammatory.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide an application of imbricate thistle extract in the preparation of medicine for treating scald.

Another object of the present invention is to provide a method for preparing a medicine for treating scalds by using the extract of thistle thistle.

The present invention is achieved in the following way: the application of the imbricate thistle extract in the preparation of medicine for treating scalds.

The said imbricate thistle extract is the water extract or polar extract of imbricate thistle.

A method for preparing a medicine for treating scalds by using imbricate thistle extract,

1) Decoct the fresh or dried thistle products with water, decoct 3 times according to the material-to-liquid ratio of 1:10, 2 hours each time, filter and extract the filtrate, combine the filtrates, and concentrate the filtrates under reduced pressure to obtain the water extract extract, stored at 4°C;

2) The fresh or dry product of thistle and the ethanol with a mass percentage of 75% were refluxed and extracted three times according to the ratio of solid to liquid 1:10, filtered, and the filtrates were combined; with petroleum ether, ethyl acetate, normal Butanol is extracted sequentially, and the extracts of each layer are combined to obtain petroleum ether layer, ethyl acetate layer and n-butanol layer extracts, which are concentrated to obtain polar extracts for later use;

3) Pack the water extract extract and the polar extract separately.

The water extract extract and polar extract are packaged separately as traditional Chinese medicine extract.

Take separate water extract extract, separate polar extract, or mixed extract of water extract and polar extract, add pharmaceutically acceptable adjuvants to make powder, film, oil, Aerosol, tincture, ointment, liniment, suspension, solution, or plaster.

1 The therapeutic effect of imbricate thistle burns is described in detail through the following animal experiments.

1.1 Experimental study on the treatment of burns with thistle water extract

48 SPF level mice, half male and half female, weighing 18-22g, were randomly divided into 4 groups according to body weight, namely water decoction group, Jingwanhong group, silver sulfadiazine group, blank group, each group 12 rats. Anesthesia was performed by inhalation anesthesia, and hair removal was performed with 8% sodium sulfide. After 24 hours, wound surface preparation was performed on the undamaged depilated area. Adjust the pressure of the YLS-5Q desktop super temperature-controlled scald instrument to 500g, and set the temperature to 80°C. Choose a hot head with a diameter of 2 cm, aim the hot head that meets the usage requirements at the intended scald area of the mouse, and time it for 12 seconds to complete the preparation of the third-degree burn wound. After the mice were injured for 2 hours, the mice were given drug treatment. They were divided into water decoction group, the positive control group was Jingwanhong group and silver sulfadiazine group, and the blank group was not administered. It is administered once a day, and after administration, the wound is treated briefly to ensure the effectiveness of the drug. The medicine was administered continuously for 12 days, and the healing condition of the wound surface was observed. The wound healing rate of the mice was calculated according to the change of the wound area of the mice. The calculation formula of wound healing rate is as follows:

1.2 Experimental study on animal burns treated with n-butanol polar parts of imbricate thistle

48 SPF level mice, half male and half female, weighing 18-22g, were randomly divided into 4 groups according to body weight, namely n-butanol group, Jingwanhong group, silver sulfadiazine group, blank group, each group 12 rats. Anesthesia was performed by inhalation anesthesia, and hair removal was performed with 8% sodium sulfide. After 24 hours, wound surface preparation was performed on the undamaged depilated area. Adjust the pressure of the YLS-5Q desktop super temperature-controlled scald instrument to 500g, and set the temperature to 80°C. Choose a hot head with a diameter of 2 cm, aim the hot head that meets the usage requirements at the intended scald area of the mouse, and time it for 12 seconds to complete the preparation of the third-degree burn wound. After the mice were injured for 2 hours, the mice were given drug treatment. They were divided into water decoction group, the positive control group was Jingwanhong group and silver sulfadiazine group, and the blank group was not given medicine. It is administered once a day, and after administration, the wound is treated briefly to ensure the effectiveness of the drug. The medicine was administered continuously for 12 days, and the healing condition of the wound surface was observed. The healing rate of the mouse wound was calculated according to the change of the wound area of the mouse. The calculation formula of wound healing rate is as follows:

1.3 Experimental study on the treatment of scald animals with the polar parts of imbricate ethyl acetate

48 SPF level mice, half male and half female, weighing 18-22g, were randomly divided into 4 groups according to body weight, namely n-butanol group, Jingwanhong group, silver sulfadiazine group, blank group, each group 12 rats. Anesthesia was performed by inhalation anesthesia, and hair removal was performed with 8% sodium sulfide. After 24 hours, wound surface preparation was performed on the undamaged depilated area. Adjust the pressure of the YLS-5Q desktop super temperature-controlled scald instrument to 500g, and set the temperature to 80°C. Choose a hot head with a diameter of 2 cm, aim the hot head that meets the usage requirements at the intended scald area of the mouse, and time it for 12 seconds to complete the preparation of the third-degree burn wound. After the mice were injured for 2 hours, the mice were given drug treatment. They were divided into water decoction group, the positive control group was Jingwanhong group and silver sulfadiazine group, and the blank group was not given medicine. It is administered once a day, and after administration, the wound is treated briefly to ensure the effectiveness of the drug. The medicine was administered continuously for 12 days, and the healing condition of the wound surface was observed. The wound healing rate of the mice was calculated according to the change of the wound area of the mice. The calculation formula of wound healing rate is as follows:

1.4 Experimental study on animal burns treated with imbricate petroleum ether polar parts

48 SPF grade white mice, half male and half female, weighing 18-22g, were randomly divided into 4 groups according to body weight, respectively petroleum ether group, Jingwanhong group, silver sulfadiazine group, blank group, each group of mice 12 only. Anesthesia was performed by inhalation anesthesia, and hair removal was performed with 8% sodium sulfide. After 24 hours, wound surface preparation was performed on the undamaged depilated area. Adjust the pressure of the YLS-5Q desktop super temperature-controlled scald instrument to 500g, and set the temperature to 80°C. Choose a hot head with a diameter of 2 cm, aim the hot head that meets the usage requirements at the intended scald area of the mouse, and time it for 12 seconds to complete the preparation of the third-degree burn wound. After the mice were injured for 2 hours, the mice were given drug treatment. They were divided into water decoction group, the positive control group was Jingwanhong group and silver sulfadiazine group, and the blank group was not given medicine. It is administered once a day, and after administration, the wound is treated briefly to ensure the effectiveness of the drug. The medicine was administered continuously for 12 days, and the healing condition of the wound surface was observed. The wound healing rate of the mice was calculated according to the change of the wound area of the mice. The calculation formula of wound healing rate is as follows:

2 Experimental results

2.1 Animal experimental research results of imbricate thistle water extract in treating scald

The experimental research results show that: compared with the positive control group, the water decoction group has a better therapeutic effect. Especially in preventing body fluid extravasation caused by burns, it has obvious advantages. The decoction group had significantly stronger therapeutic effects on third-degree burn wounds than the Jingwanhong group and silver sulfadiazine group, and 6 days before the wound recovered, the water decoction group could accelerate the recovery of the wound and significantly increase the healing rate of the wound. The results are shown in table 1, table 2, accompanying drawing 1, and accompanying drawing 2.

Table 1 Changes in area of third-degree burn wounds in mice (cm ² )

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

Table 2 Healing rate of third-degree burn wounds in mice (%)

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

2.2 Animal experimental research results on the treatment of scalds with n-butanol polar parts of imbricate thistle

Experimental research result shows: n-butanol group and positive control group all show the therapeutic effect to scald, and in the early stage of experiment, n-butanol group advantage is obvious, the result is shown in table 3, table 4, accompanying drawing 3, accompanying drawing 4 Show.

Table 3 Changes in area of third-degree burn wounds in mice (cm ² )

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

Table 4 Healing rate of third-degree burn wounds in mice (%)

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

2.3 The results of animal experiments on the treatment of scalds with the polar parts of imbricate ethyl acetate

Experimental research result shows: ethyl acetate group and positive control group all show the therapeutic effect to scald, and therapeutic effect is quite, and result is shown in table 5, table 6, accompanying drawing 5, accompanying drawing 6.

Table 5 Changes in area of third-degree burn wounds in mice (cm ² )

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine,

▲▲p<0.01, ▲p<0.05)

Table 6 Wound healing rate of third-degree burns in mice (%)

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

2.4 The results of animal experiments on scalds treated by the polar parts of imbricate petroleum ether

The experimental research results show that: compared with the blank group, the petroleum ether group has a certain healing effect on burns and scalds, but the effect is lower than that of the positive control group. The results are shown in Table 7, Table 8, accompanying drawing 7, and accompanying drawing 8.

Table 7 Changes in area of third-degree burn wounds in mice (cm ² )

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

Table 8 Healing rate of third-degree burn wounds in mice (%)

(Note: Compared with blank group, **P<0.01, *P<0.05; compared with Jingwanhong, ++p<0.01, +p<0.05; compared with silver sulfadiazine, ▲▲p<0.01, ▲p <0.05)

The results of animal experiments on the treatment of scalds with thistles showed that the water decoction of thistles and its alcohol extracts with different polarities have therapeutic effects on scalds, and the decoction has the most obvious effect. In summary, the order of the curative effect of thistle on scalds is as follows: water decoction group>Jingwanhong group>silver sulfadiazine group≥ethyl acetate group>n-butanol group>petroleum ether group.

The present invention finds imbricate thistle as the active ingredient of medicine, and experiments prove that imbricate thistle has a significant healing effect on scalds, and it is not easy to leave scars after the wound heals. It also has the function of preventing infection of scalded wounds, and can also accelerate Scabs on the scald wound surface can shrink the wound surface and heal the wound surface. Compared with the existing commonly used scald medicines, it has a better therapeutic effect, especially in preventing the body fluid extravasation caused by the scald, it has obvious advantages.

Description of drawings

Accompanying drawing 1 is the curve diagram of the area change of mouse III degree burn wound;

Accompanying drawing 2 is the curvilinear graph of wound healing rate of mouse III degree burn;

Accompanying drawing 3 is the curve diagram of the area change of mouse III degree burn wound;

Accompanying drawing 4 is the curvilinear chart of wound healing rate of mouse III degree burn;

Accompanying drawing 5 is the curve diagram of the area change of mouse III degree burn wound;

Accompanying drawing 6 is the curvilinear chart of wound healing rate of mouse III degree burn;

Accompanying drawing 7 is the curve diagram of the area change of mouse III degree burn wound;

Accompanying drawing 8 is the curve chart of wound healing rate of mice with third-degree burns.

Detailed ways

Embodiment 1 of the present invention: a method for preparing a medicine for treating scalds by using an imbricate thistle extract,

1) Decoct the fresh or dried thistle products with water, decoct 3 times according to the material-to-liquid ratio of 1:10, 2 hours each time, filter and extract the filtrate, combine the filtrates, and concentrate the filtrates under reduced pressure to obtain the water extract extract, stored at 4°C;

2) The fresh or dry product of thistle and the ethanol with a mass percentage of 75% were refluxed and extracted three times according to the ratio of solid to liquid 1:10, filtered, and the filtrates were combined; with petroleum ether, ethyl acetate, normal Butanol is extracted sequentially, and the extracts of each layer are combined to obtain petroleum ether layer, ethyl acetate layer and n-butanol layer extracts, which are concentrated to obtain polar extracts for later use;

3) Pack the water extract extract and the polar extract separately.

Embodiment 2 of the present invention: further process the water extract extract and polar extract prepared in Example 1; take separate water extract extract, independent polar extract, or water extract extract and Mixed extracts of polar extracts, adding pharmaceutically acceptable adjuvants to make powders, films, oils, aerosols, tinctures, ointments, liniments for external use, suspensions, solutions or plasters.

When in use, directly apply the prepared medicine evenly to the affected area once a day, and the wound does not need to be cleaned.

Claims (3)

1. a watt Ji extract preparation treatment scald medicament method is covered in a kind of utilization, it is characterised in that:

1) watt Ji fresh goods will be covered or dry product adds water to cook, decocted 3 times, each 2h by solid-liquid ratio 1:10, filtrate is extracted in filtering, is merged Filtrate is made water extract medicinal extract, saves at 4 DEG C by filtrate after being concentrated under reduced pressure；

2) ethyl alcohol that watt Ji fresh goods or dry product and quality percentage are 75% will be covered and carries out refluxing extraction 3 in the ratio of solid-liquid ratio 1:10 It is secondary, it filters, after merging filtrate；It is successively extracted with petroleum ether, ethyl acetate, n-butanol, and merges each layer extract liquor, obtain petroleum ether Layer, ethyl acetate layer and n-butanol layer extracting solution, it is spare that concentration obtains polarity medicinal extract；

3) water extract medicinal extract and polarity medicinal extract are individually packed.

2. a watt Ji extract preparation treatment scald medicament method is covered in utilization according to claim 1, it is characterised in that: by water Extract medicinal extract and polarity medicinal extract are individually packaged as Chinese medical concrete.

3. a watt Ji extract preparation treatment scald medicament method is covered in utilization according to claim 1, it is characterised in that: take list The mixing medicinal extract of only water extract medicinal extract, individual polarity medicinal extract or water extract medicinal extract and polarity medicinal extract, addition can pharmaceutically connect Powder, film, finish, aerosol, tincture, ointment, externally-applied liniment, suspension, solution or emplastrum is made in the adjuvant received.