CN106259891A - A kind of probiotic bacteria buccal tablet and preparation method thereof - Google Patents
A kind of probiotic bacteria buccal tablet and preparation method thereof Download PDFInfo
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- CN106259891A CN106259891A CN201510282113.XA CN201510282113A CN106259891A CN 106259891 A CN106259891 A CN 106259891A CN 201510282113 A CN201510282113 A CN 201510282113A CN 106259891 A CN106259891 A CN 106259891A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 73
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 73
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 73
- 229940046011 buccal tablet Drugs 0.000 title claims abstract description 49
- 239000006189 buccal tablet Substances 0.000 title claims abstract description 49
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 238000000855 fermentation Methods 0.000 claims abstract description 35
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- 238000002156 mixing Methods 0.000 claims description 15
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
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- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 abstract description 7
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- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a kind of probiotic bacteria buccal tablet and preparation method thereof, including: after A) milk powder of 60～80 weight portions being mixed with water, sterilizing, after cooling, add probiotic bacteria strain, ferment, obtain fermentation liquid；In described fermentation liquid, the content of probiotic bacteria is 0.1～5 weight portions；B) in described fermentation liquid, add the edible glue of 1～6 weight portions and the starch of 5～8 weight portions, be mixed to get mixed liquor；C) by described mixed liquor injection mould carries out lyophilization, probiotic bacteria buccal tablet after the demoulding, is obtained.Compared with prior art, the present invention is by the content of various components in regulation probiotic bacteria buccal tablet, the most freeze-dried molding i.e. can get probiotic bacteria buccal tablet, preparation method is simple, simultaneously because lyophilization is low-temperature vacuum environment, probiotic bacteria can be alleviated and destroy biological activity because of heated drying, and due to water activity low under conditions of, probiotic bacteria can be made to present resting state, maintain stablizing of product shelf phase.
Description
Technical field
The invention belongs to probiotic composition and solid beverage based article technical field, particularly relate to a kind of prebiotic
Bacterium buccal tablet and preparation method thereof.
Background technology
The tablet dosage form that buccal tablet slowly dissolves in referring to be contained in oral cavity, being applied to medicine aspect can be to oral cavity
And the drug effect that pharyngeal generation is lasting, there is the effects such as antiinflammatory sterilization, be applied to confection and put and show off, can help
Oral cavity carry out abnormal flavour cover, the effect of cleaning one's teeth etc..Mostly buccal tablet in terms of confection is the fruit of taste-aromatic
Herba Menthae slice, Herba Menthae has extremely strong sterilization antibacterial action, and often taking can preventing viral flu, oral cavity
Disease etc., and also can make breath freshening.
Modern medicine think 80% halitosis be by oral cavity anaerobium excessive multiplication, decompose food in digestion oral cavity
Residue, Organic substance and blood, produce caused by volatile sulfide.And oral cavity probiotic bacteria can be to anaerobe
Breeding produce powerful restraint, periodontal disease, oral cavity recurrent ulcer can be prevented, be directed to halitosis
Root.
Probiotic bacteria is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system
In, definite health efficacy can be produced, improve host's microecological balance, the activity playing beneficial effect is useful
The general name of microorganism.At present, the research about probiotic bacteria is allocated as many aspects, and one, from the sieve of strain
Choosing is identified, except basis source, and such as feces, breast milk etc., also have and analyze institute according to gene identification
The strain belonged to and bacterial strain；Its two, develop into commercially available prod from application end and study, according to different dosage form,
Define it both effectiveness, and then application health care certification.Different probiotic bacterias has following effect: 1, improve
Allergy, eczema, have the symptom that can reduce baby's atopic eczema, and prevention eczema, diaper rash waited
Quick property disease；2, prevent, reduce baby's respiratory tract infection risk, effective reduction respiratory tract infection and 1 years old
The risk of front repeated infection；3, baby's resistance, immunity are strengthened；4, the use of antibiotic is reduced.
At present, buccal tablet mainly uses gas phase instantaneous high pressure to prepare, and processing step is various, sample broke rate
Higher, pressure transient, quality management and control is more difficult, day production capacity relatively low.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is to provide a kind of probiotic bacteria buccal tablet and system thereof
Preparation Method, preparation technology is simple for this buccal tablet.
The invention provides a kind of probiotic bacteria buccal tablet, the freeze-dried molding of following component obtain:
Preferably, the maltose of 4～6 weight portions is also included.
Preferably, the white sugar of 3～4 weight portions is also included.
Preferably, described edible glue is gelatin and/or pectin.
Preferably, the freeze-dried molding of following component obtain；
Preferably, the moisture of described probiotic bacteria buccal tablet is less than or equal to 5%.
Preferably, the water of described probiotic bacteria buccal tablet is lived less than or equal to 0.3.
Present invention also offers the preparation method of a kind of probiotic bacteria buccal tablet, including:
A) after the milk powder of 60～80 weight portions being mixed with water, sterilizing, after cooling, add probiotic bacteria strain,
Ferment, obtain fermentation liquid；In described fermentation liquid, the content of probiotic bacteria is 0.1～5 weight portions；
B) in described fermentation liquid, the edible glue of 1～6 weight portions and the starch of 5～8 weight portions, mixing are added
Obtain mixed liquor；
C) by described mixed liquor injection mould carries out lyophilization, probiotic bacteria buccal tablet after the demoulding, is obtained.
Preferably, the solid content of described mixed liquor is Brix30%～35%.
Preferably, described cryodesiccated vacuum is 5～20MPa；Temperature is-37 DEG C～-30 DEG C.
The invention provides a kind of probiotic bacteria buccal tablet and preparation method thereof, including: A) by 60～80 weight
After the milk powder of part mixes with water, sterilizing, after cooling, add probiotic bacteria strain, ferment, sent out
Ferment liquid；In described fermentation liquid, the content of probiotic bacteria is 0.1～5 weight portions；B) add in described fermentation liquid
The edible glue of 1～6 weight portions and the starch of 5～8 weight portions, be mixed to get mixed liquor；C) by described mixing
Liquid injects in mould and carries out lyophilization, obtains probiotic bacteria buccal tablet after the demoulding.Compared with prior art,
The present invention is by the content of various components in regulation probiotic bacteria buccal tablet, more freeze-dried molding
To probiotic bacteria buccal tablet, preparation method is simple, simultaneously because lyophilization is low-temperature vacuum environment, can delay
Solve probiotic bacteria and destroy biological activity because of heated drying, and due to water activity low under conditions of, can make prebiotic
Bacterium presents resting state, maintains stablizing of product shelf phase.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Describe, it is clear that described embodiment is only a part of embodiment of the present invention rather than all wholely
Embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creativeness
The every other embodiment obtained under work premise, broadly falls into the scope of protection of the invention.
The invention provides a kind of probiotic bacteria buccal tablet, the freeze-dried molding of following component obtain:
Wherein, the content of described probiotic bacteria is preferably 0.1～3 weight portions, more preferably 0.1～1 weight portion,
It is further preferably 0.1～0.5 weight portion, most preferably 0.1～0.2 weight portion；The kind of described probiotic bacteria is this
Probiotic bacteria kind known to skilled person, there is no special restriction, is preferably breast in the present invention
Acid bacterium and/or bacillus bifidus.
The content of described milk powder is preferably 65～80 weight portions, more preferably 70～80 weight portions, further preferably
It is 75～80 weight portions；Described milk powder is preferably whole milk powder.
The content of described edible glue is preferably 2～6 weight portions；The kind of described edible glue is art technology
Edible glue known to personnel, there is no special restriction, and the present invention is preferably gelatin and/or pectin, more
It is preferably gelatin and pectin；Wherein, the content of described gelatin preferably 1～3 weight portion, containing of described pectin
Amount is preferably 1～3 weight portions.
The content of described starch is preferably 6～7 weight portions.
According to the present invention, in order to regulate the mouthfeel of probiotic bacteria buccal tablet, the present invention the most also includes 4～6 weights
The maltose of amount part.The maltose that the kind of described maltose is well known to those skilled in the art, and
Without special restriction, the present invention is preferably oligomeric isomaltose.In oligomeric isomaltose can promote human body
Bacillus bifidus is significantly bred, and has water soluble dietary fiber function, and calorific value is low, anti-caries etc..
The present invention the most also includes the white sugar of 3～4 weight portions.
Probiotic bacteria buccal tablet of the present invention is obtained by the freeze-dried molding of above composition.Described probiotic bacteria is sucked
The moisture of sheet is preferably smaller than equal to 5%；The water of described probiotic bacteria buccal tablet is lived less than or equal to 0.3.
Present invention also offers the preparation method of a kind of above-mentioned probiotic bacteria buccal tablet, including: A) by 60～80
After the milk powder of weight portion mixes with water, sterilizing, after cooling, add probiotic bacteria strain, ferment,
To fermentation liquid；In described fermentation liquid, the content of probiotic bacteria is 0.1～5 weight portions；B) in described fermentation liquid
Add the edible glue of 1～6 weight portions and the starch of 5～8 weight portions, be mixed to get mixed liquor；C) by described
Mixed liquor injects in mould and carries out lyophilization, obtains probiotic bacteria buccal tablet after the demoulding.
Wherein, the source of all raw materials is not particularly limited by the present invention, for commercially available.Described
Milk powder, probiotic bacteria, edible glue are the most same as above with starch, do not repeat them here.
The milk powder of 60～80 weight portions is mixed with water, be preferably additionally added 3～4 weight portions white sugar and/or
The maltose of 4～6 weight portions；Described white sugar and maltose are the most same as above, do not repeat them here.Described
The temperature of mixing is preferably 40 DEG C～60 DEG C, more preferably 50 DEG C～60 DEG C；The time of described mixing is preferably
10～60min, more preferably 20～40min.
In order to make the more homogeneous mixing of milk powder and liquid phase feed, the most also homogenize after present invention mixing
Process；The described temperature processed that homogenizes is preferably 50 DEG C～80 DEG C, more preferably 60 DEG C～70 DEG C；Described
The pressure processed that homogenizes is preferably 150～300Bar, and more preferably 150～250Bar, be further preferably warp
Crossing two sections of process that homogenize, the one section of pressure processed that homogenizes is preferably 150～200Bar, and two-stage nitration homogenizes
The pressure processed is preferably 200～250Bar.Through homogenizing process, the more continuous cunning of mixed liquor mouthfeel can be made,
Butterfat granule is more careful.
Homogenize after processing, sterilizing.The method that the method for described sterilizing is well known to those skilled in the art is i.e.
Can, there is no special restriction, present invention preferably employs 120 DEG C～150 DEG C of high-temperature short-time sterilization；Described sterilizing
Time be preferably 25～35s.By the removable miscellaneous bacteria of sterilizing, it is to avoid impact fermentation.
Sterilizing, after cooling, adds probiotic bacteria strain, ferments, obtain fermentation liquid.Described probiotic bacteria
The probiotic bacteria strain that strain is well known to those skilled in the art, there is no special restriction, and the present invention is excellent
Elect lactic acid bacteria and/or bacillus bifidus as.Add probiotic bacteria strain whole process to operate the most in an aseptic environment.Described
The temperature of fermentation is preferably 42 DEG C～45 DEG C；The time of described fermentation is preferably 3～6h.
The fermentation liquid obtained preferably is stirred crushing by the present invention, to destroy yeasting, is subsequently adding
The edible glue of 1～6 weight portions and the starch of 5～8 weight portions, be mixed to get mixed liquor.Described starch is the most pre-
It is subsequently adding after gelatinizing；The solid content of described mixed liquor is preferably Brix30%～35%, more preferably
Brix32%～34%.
Being injected in mould by described mixed liquor and carry out lyophilization, described cryodesiccated vacuum is 5～20
MPa, more preferably 8～15MPa；Described cryodesiccated temperature is preferably-37 DEG C～-30 DEG C.
After lyophilization, the demoulding i.e. can get probiotic bacteria buccal tablet.
The present invention is by the content of various components in regulation probiotic bacteria buccal tablet, more freeze-dried molding is i.e.
Available probiotic bacteria buccal tablet, preparation method is simple, simultaneously because lyophilization is low-temperature vacuum environment,
Probiotic bacteria can be alleviated and destroy biological activity because of heated drying, and due to water activity low under conditions of, can make
Probiotic bacteria presents resting state, maintains stablizing of product shelf phase.
In order to further illustrate the present invention, a kind of probiotic bacteria mouth present invention provided below in conjunction with embodiment
Buccal tablet and preparation method thereof is described in detail.
Reagent used in following example is commercially available.
Embodiment 1
1.1 by 60 DEG C of the water of the whole milk powder of 60 weight portions, the white sugar of 3 weight portions and 200 weight portions
Mixing 30min, obtains emulsion；Then emulsion is heated to 70 DEG C, is respectively by two sections of homogenization pressures
180Bar and 240Bar carries out the process that homogenizes, and the feed liquid after then homogenizing is cooled to 50 DEG C.
Feed liquid after 1.2 coolings that will obtain in 1.1 passes through 135 DEG C of high temperatures, and the time is 25～35
s。
Feed liquid after 1.3 sterilizations that will obtain in lactobacillus inoculum to 1.2 in an aseptic environment is fermented,
The temperature of fermentation is 43 DEG C, and the time of fermentation is 6h, is 4.4 to pH value, obtains fermentation liquid.
1.4 fermentation liquids obtained in 1.3 add the pectin of 1 weight portion, the gelatin of 3 weight portions and 7
The starch mixing of weight portion pre-gelatinized, change solid content environment, to Brix30%, is then injected in mould carrying out
Lyophilization, freezing environment vacuum 10MPa, temperature is-30 DEG C, is dried to sample moisture less than 5%,
Water is lived less than 0.3, obtains probiotic bacteria buccal tablet after the demoulding.
Embodiment 2
2.1 by 60 DEG C of the water of the whole milk powder of 75 weight portions, the white sugar of 4 weight portions and 250 weight portions
Mixing 30min, obtains emulsion；Then emulsion is heated to 70 DEG C, is respectively by two sections of homogenization pressures
180Bar and 240Bar carries out the process that homogenizes, and the feed liquid after then homogenizing is cooled to 50 DEG C.
Feed liquid after 2.2 coolings that will obtain in 2.1 passes through 135 DEG C of high temperatures, and the time is 25～35
s。
2.3 in an aseptic environment bacillus bifidus is inoculated into the sterilization obtained in 2.2 after feed liquid in carry out send out
Ferment, the temperature of fermentation is 45 DEG C, and the time of fermentation is 6h, is 4.4 to pH value, obtains fermentation liquid.
2.4 fermentation liquids obtained in 2.3 add the pectin of 2 weight portions, the gelatin of 3 weight portions and 8
The starch mixing of weight portion pre-gelatinized, change solid content environment, to Brix32%, is then injected in mould carrying out
Lyophilization, freezing environment vacuum 10MPa, temperature is-30 DEG C, is dried to sample moisture less than 5%,
Water is lived less than 0.3, obtains probiotic bacteria buccal tablet after the demoulding.
Embodiment 3
3.1 by 60 DEG C of the water of the whole milk powder of 80 weight portions, the white sugar of 3 weight portions and 200 weight portions
Mixing 30min, obtains emulsion；Then emulsion is heated to 70 DEG C, is respectively by two sections of homogenization pressures
180Bar and 240Bar carries out the process that homogenizes, and the feed liquid after then homogenizing is cooled to 50 DEG C.
Feed liquid after 3.2 coolings that will obtain in 3.1 passes through 135 DEG C of high temperatures, and the time is 25～35
s。
Feed liquid after 3.3 sterilizations that will obtain in lactobacillus inoculum to 3.2 in an aseptic environment is fermented,
The temperature of fermentation is 45 DEG C, and the time of fermentation is 4h, is 4.4 to pH value, obtains fermentation liquid.
3.4 fermentation liquids obtained in 3.3 add the pectin of 2 weight portions, the gelatin of 2 weight portions and 6
The starch mixing of weight portion pre-gelatinized, change solid content environment, to Brix35%, is then injected in mould carrying out
Lyophilization, freezing environment vacuum 10MPa, temperature is-30 DEG C, is dried to sample moisture less than 5%,
Water is lived less than 0.3, obtains probiotic bacteria buccal tablet after the demoulding.
Comparative example 1
1.1 by 60 DEG C of the water of the whole milk powder of 60 weight portions, the white sugar of 3 weight portions and 200 weight portions
Mixing 30min, obtains emulsion；Then emulsion is heated to 70 DEG C, is respectively by two sections of homogenization pressures
180Bar and 240Bar carries out the process that homogenizes, and the feed liquid after then homogenizing is cooled to 50 DEG C.
Feed liquid after 1.2 coolings that will obtain in 1.1 passes through 135 DEG C of high temperatures, and the time is 25～35
s。
Feed liquid after 1.3 sterilizations that will obtain in lactobacillus inoculum to 1.2 in an aseptic environment is fermented,
The temperature of fermentation is 43 DEG C, and the time of fermentation is 6h, is 4.4 to pH value, obtains fermentation liquid.
1.4 fermentation liquids obtained in 1.3 add the pectin of 1 weight portion, the gelatin of 3 weight portions and 7
The starch mixing of weight portion pre-gelatinized, lyophilization obtains powder, uses gas phase instantaneous high pressure to obtain prebiotic
Bacterium buccal tablet.
To lactic acid bacteria, bacillus bifidus, embodiment 1 obtaining probiotic bacteria buccal tablet, embodiment 2 obtain
Probiotic bacteria buccal tablet and comparative example 1 in the buccal tablet that obtains carry out room temperature (25 DEG C) and preserve experiment, knot
Fruit is as shown in table 1.
Table 1 room temperature preserves experimental result
Heating lactic acid bacteria and bacillus bifidus and maltreat (37 DEG C) preservation experiment, result is as shown in table 2.
Table 2 is heated and is maltreated preservation experimental result
The probiotic bacteria buccal tablet obtained in embodiment 1 is detected, its every physical and chemical index and microorganism
Index result is as shown in table 3.
Table 3 embodiment 1 probiotic bacteria buccal tablet testing result
Claims (10)
1. a probiotic bacteria buccal tablet, it is characterised in that obtained by the freeze-dried molding of following component:
Probiotic bacteria buccal tablet the most according to claim 1, it is characterised in that also include 4～6 weight
The maltose of part.
Probiotic bacteria buccal tablet the most according to claim 1, it is characterised in that also include 3～4 weight
The white sugar of part.
Probiotic bacteria buccal tablet the most according to claim 1, it is characterised in that described edible glue is bright
Glue and/or pectin.
Probiotic bacteria buccal tablet the most according to claim 1, it is characterised in that by following component through cold
Freeze drying and moulding to obtain；
Probiotic bacteria buccal tablet the most according to claim 1, it is characterised in that described probiotic bacteria is sucked
The moisture of sheet is less than or equal to 5%.
Probiotic bacteria buccal tablet the most according to claim 1, it is characterised in that described probiotic bacteria is sucked
The water of sheet is lived less than or equal to 0.3.
8. the preparation method of a probiotic bacteria buccal tablet, it is characterised in that including:
A) after the milk powder of 60～80 weight portions being mixed with water, sterilizing, after cooling, add probiotic bacteria strain,
Ferment, obtain fermentation liquid；In described fermentation liquid, the content of probiotic bacteria is 0.1～5 weight portions；
B) in described fermentation liquid, the edible glue of 1～6 weight portions and the starch of 5～8 weight portions, mixing are added
Obtain mixed liquor；
C) by described mixed liquor injection mould carries out lyophilization, probiotic bacteria buccal tablet after the demoulding, is obtained.
Preparation method the most according to claim 8, it is characterised in that the solid content of described mixed liquor
For Brix30%～35%.
Preparation method the most according to claim 8, it is characterised in that described cryodesiccated very
Reciprocal of duty cycle is 5～20MPa；Temperature is-37 DEG C～-30 DEG C.
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| CN201510282113.XA CN106259891A (en) | 2015-05-28 | 2015-05-28 | A kind of probiotic bacteria buccal tablet and preparation method thereof |
| TW105106397A TWI633843B (en) | 2015-05-28 | 2016-03-02 | Probiotic bacterial buccal tablet and preparation method thereof |
| JP2016051023A JP2016220674A (en) | 2015-05-28 | 2016-03-15 | Probiotic oral tablet and method for producing the same |
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Cited By (1)
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|---|---|---|---|---|
| CN107114794A (en) * | 2017-06-06 | 2017-09-01 | 上海真合生物技术有限公司 | Probiotic composition for strengthening antiallergy ability |
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| BR112021016587A2 (en) | 2019-02-22 | 2021-11-03 | Aquero Canada Ltd | Fortified milk compositions and their preparation processes |
| BR112021026747A2 (en) * | 2019-07-09 | 2022-05-10 | Aquero Canada Ltd | Compositions, production processes, sterilization, and health-promoting uses of freeze-dried milk |
| JP7514926B2 (en) | 2019-12-06 | 2024-07-11 | 健偉 倪 | Composition and use having the function of adjusting the diversity of individualized intestinal microbiota |
| CN112293728A (en) * | 2020-07-13 | 2021-02-02 | 丽江美之源食品有限公司 | Fructus phyllanthi probiotic buccal tablet |
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Also Published As
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| JP2016220674A (en) | 2016-12-28 |
| TW201641021A (en) | 2016-12-01 |
| TWI633843B (en) | 2018-09-01 |
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