CN106248919A - Method based on blood sample vibration detection coagulation - Google Patents
Method based on blood sample vibration detection coagulation Download PDFInfo
- Publication number
- CN106248919A CN106248919A CN201610560461.3A CN201610560461A CN106248919A CN 106248919 A CN106248919 A CN 106248919A CN 201610560461 A CN201610560461 A CN 201610560461A CN 106248919 A CN106248919 A CN 106248919A
- Authority
- CN
- China
- Prior art keywords
- blood sample
- vibration
- capillary tube
- meniscus
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 88
- 239000008280 blood Substances 0.000 title claims abstract description 88
- 238000000034 method Methods 0.000 title claims abstract description 47
- 238000001514 detection method Methods 0.000 title claims abstract description 44
- 230000015271 coagulation Effects 0.000 title claims abstract description 27
- 238000005345 coagulation Methods 0.000 title claims abstract description 27
- 230000005499 meniscus Effects 0.000 claims abstract description 40
- 230000008859 change Effects 0.000 claims abstract description 26
- 230000008569 process Effects 0.000 claims abstract description 15
- 230000023555 blood coagulation Effects 0.000 claims description 16
- 229910052710 silicon Inorganic materials 0.000 claims description 10
- 239000010703 silicon Substances 0.000 claims description 10
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000013307 optical fiber Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 6
- 230000007246 mechanism Effects 0.000 claims description 4
- 230000005484 gravity Effects 0.000 claims description 3
- 210000005239 tubule Anatomy 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 2
- 230000023597 hemostasis Effects 0.000 abstract description 8
- 238000013461 design Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 description 10
- 230000003287 optical effect Effects 0.000 description 8
- 208000007536 Thrombosis Diseases 0.000 description 7
- 239000011324 bead Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 7
- 230000005291 magnetic effect Effects 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 5
- 230000005298 paramagnetic effect Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000007711 solidification Methods 0.000 description 3
- 230000008023 solidification Effects 0.000 description 3
- 206010053567 Coagulopathies Diseases 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000035602 clotting Effects 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005672 electromagnetic field Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 201000001320 Atherosclerosis Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000386 athletic effect Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011246 composite particle Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003527 fibrinolytic agent Substances 0.000 description 1
- 230000003480 fibrinolytic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4905—Determining clotting time of blood
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of method based on blood sample vibration detection coagulation, blood sample to be detected is added in the capillary tube of pendency, to produce the blood sample drop of the band meniscus connected by surface tension at the mouth of pipe of capillary tube lower end；On the capillary arrange vibration generation cavity connect with capillary tube, by the capillary tube other end close, then vibration generation cavity on apply thrust alternately with suction so that blood sample drop meniscus generation of reciprocating vibration；The change in location of blood sample drop meniscus bottom in detection vibration processes, and calculate the elastic modelling quantity of blood sample, thus realize coagulation detection.Hemostasis examination result of the present invention is more accurate, more simplifies according to the structure of the detecting device of method design, it is possible to achieve on-line real-time measuremen.
Description
Technical field
The present invention relates to Hemostasis examination, be specifically related to a kind of method based on blood sample vibration detection coagulation, belong to blood
Liquid detection technique field.
Background technology
Human body has complicated and complete hemostasis, blood coagulation and anticoagulant mechanism, and the formation of thrombosis is relevant with whole clotting mechanism,
And the research of thrombosis relates to generation and the development of the multiple diseases such as atherosclerosis, coronary heart disease, myocardial infarction；Coagulation analysis
The project checked be widely used in hemorrhage, thrombotic disease and the diagnosis of Pre-thrombosis State, observation of curative effect, anticoagulant dosage and
Prognostic analysis, the various primary or diagnosis of Secondary cases fibrinolytic and observation of curative effect.Automatically coagulation analysis refers to be divided by automatic blood coagulation
Analyzer or other analysers are analyzed to hemostasis and Related Component in thrombosis system and are applied to hemorrhage, thrombotic disease and examine
Break, treat and Index for diagnosis；The application of Automatic coagulometer so that hemostasis and thrombosis item inspection become easy, accurate, can do
Go out multinomial quantitative result accurately.
Full automatic blood-coagulation detects at present mainly following several method: 1, optical method；2, paramagnetic particle method；3, immunoturbidimetry；
The Cleaning Principle of optical method is to determine setting time by the change of detection blood sample turbidity to be measured.Treat test sample
This is during solidification, and the component content of blood all there occurs change, according to lambert's beer's law, when a branch of parallel list
When coloured light is perpendicular through the extinction material of uniform non-scatter, its absorbance just becomes with concentration and the absorber thickness of extinction material
Ratio.When blood constituent changes, the intensity of transmission light and scattered light also can change, and can be used to according to this characteristic optical method
Carry out Hemostasis examination.The shortcoming of optical method is susceptible to interference, the bubble in the fineness of test cup, blood sample, sample to be tested
Turbidity higher (such as situations such as jaundice, hyperlipidemia) all can become the interference factor of measurement.
Paramagnetic particle method refers to add magnetic bead in the sample during detection, utilizes the motion of instrument monitoring magnetic bead thus reacts blood coagulation shape
The detection method of condition.In Blood Coagulation Process, the change of blood viscosity can produce impact to magnetic bead motion, can pass through instrument
Detect this change, thus measure the coagulation of blood sample.The shortcoming of paramagnetic particle method is that Cleaning Principle is complicated, to detection knot
Fruit produces the factor of impact and includes: the degree of sample, and magnetic bead drives coagulated mass motion, and wall of cup and the technique etc. of magnetic bead.
Immunoturbidimetry refers to, when the monochromatic light that coagulo meter light source part produces passes through blood plasma to be checked, add people's examination in specimen
After agent, antigen and antibody response will form composite particles so that the turbidity of specimen to be checked increases, then the light intensity meeting passed through
Weaken.The degree that light intensity changes becomes certain quantitative relation with the amount of antigen, utilize antigen body specific binding instead
Should come detected blood sample to carry out qualitative and quantitative determination, to determine every Solidification Parameters.The shortcoming of immunoturbidimetry is mesh
The blood clotting project that front immunization can detect is less, makes the function of instrument be restricted.Reagent price is high simultaneously, improves measurement
Cost.
Summary of the invention
For deficiencies of the prior art, it is an object of the invention to provide a kind of solidifying based on blood sample vibration detection
The method of blood state, Hemostasis examination result of the present invention is more accurate, more simplifies according to the structure of the detecting device of method design, Ke Yishi
Existing on-line real-time measuremen.
The technical scheme is that and be achieved in that:
Method based on blood sample vibration detection coagulation, adds in the capillary tube of pendency by blood sample to be detected, with
The blood sample drop of the band meniscus connected by surface tension is produced at the mouth of pipe of capillary tube lower end；Vibration is set on the capillary
The capillary tube other end, to connect with capillary tube, is closed by generation cavity, then applies thrust alternately and suction on vibration generation cavity
Power is so that blood sample drop meniscus produces of reciprocating vibration；In detection vibration processes, the position of blood sample drop meniscus bottom becomes
Change, and calculate the elastic modelling quantity of blood sample, thus realize coagulation detection.
This method uses following detection device to detect, and detection device includes vibrational system and the big portion of detecting system two
Point；Described capillary tube and vibration generation cavity are vibrational system component parts, and outside defeathering tubule and vibration generation cavity, vibrational system is also
Including sample introduction tank, sample presentation pipe, sacculus and vibration power, sample introduction tank is connected with capillary tube by sample presentation pipe, to be added by sample introduction tank
Add blood sample and at the mouth of pipe of capillary tube lower end, produce the blood sample drop of the band meniscus connected by surface tension；At sample presentation pipe
It is provided with check valve so that blood sample can only be flowed toward capillary tube direction by sample introduction tank and can not flow backwards；Vibration generation cavity is located at list
Connecting on valve with intercapillary sample presentation pipe and with capillary tube, described sacculus is arranged on vibration generation cavity, vibrates power
It is connected with sacculus by drive mechanism so that sacculus produces reciprocating motion, and then makes blood sample drop meniscus up-down vibration；Capillary
The size of pipe is so that Surface Tension of Liquid Drops is applied to the thrust sum of drop more than drop gravity and vibration power, so that drop
Reciprocating motion will not be dripped；
Described detecting system includes daylighting module and the signal processing module that light emitting module is relative with light emitting module, luminous
Module is positioned at capillary tube lower end vertical with capillary tube with daylighting module line, and the light beam that light emitting module sends is curved from blood sample drop
Lunar surface side is irradiated in the daylighting module of meniscus opposite side, and the light detected in daylighting module impinges upon meniscus and air
Intersection have a boundary signal, demarcated in daylighting module the change of signal position by detection meniscus, and
Output, to signal processing module, thus detects the change of capillary meniscus, corresponding calculating blood elastic modelling quantity, thus traces
Coagulation process.
Described daylighting module is made up of silicon cell and optical fiber flexible pipe, and optical fiber flexible pipe one end is connected on silicon cell, separately
One end is towards light emitting module.
Described vibration power is air pump, and the piston of air pump is connected with sacculus, and air pump is connected with electromagnetic valve with by electromagnetic valve control
Air pump operating frequency processed.
Compared with prior art, there is advantages that
1, compared with optical method, the impact of the bubble in present invention fineness the most test tube, blood, can improve detection essence
Degree.
2, the electromagnetic field change caused by magnetic bead motion needs to reach certain motion amplitude (> 5mm) just can cause examining
The electromagnetic field parameters change surveyed, therefore the reciprocating frequency of vibration of magnetic bead can not be the highest, causes Blood Coagulation Process
Sample frequency also suffers restraints, and typically up to can only achieve 1-2Hz sample frequency.The present invention is compared with paramagnetic particle method, due to optics
Detection has high sensitivity and high-speed response characteristic, therefore can apply higher frequency of vibration to blood sample, and therefore the present invention is for solidifying
The temporal resolution that blood state is traced is higher than paramagnetic particle method, and sample frequency, up to more than 30Hz, substantially increases accuracy of detection.
3, present invention detection is the blood elastic modelling quantity the most relevant to coagulation, compared with immunoturbidimetry, no
Need to add antibody, reduce cost, by compositions etc. such as Blood Cholesterol, blood glucose, blood fat, light transmittance is not affected simultaneously,
Accuracy of detection can be improved further.
4, the present invention can reduce the design difficulty of vibrational system, simplifies structure, improves blood coagulation analyzer internal structure
Stability, it is achieved be easier to.
Accompanying drawing explanation
The structure of the detecting device schematic diagram that Fig. 1-detection method is corresponding.
Fig. 2-Cleaning Principle figure of the present invention.
The Cleaning Principle figure of Fig. 3-stereoscopic-state of the present invention.
Detailed description of the invention
With detailed description of the invention, the present invention is described in further detail below in conjunction with the accompanying drawings.
Present invention method based on blood sample vibration detection coagulation, adds the capillary tube of pendency to by blood sample to be detected
In, to produce the blood sample drop of the band meniscus connected by surface tension at the mouth of pipe of capillary tube lower end；On the capillary
Arrange vibration generation cavity connect with capillary tube, by the capillary tube other end close, then vibration generation cavity on apply replace
Thrust with suction so that blood sample drop meniscus produce of reciprocating vibration；Blood sample drop meniscus bottom in detection vibration processes
Change in location, and calculate the elastic modelling quantity of blood sample, thus realize coagulation detection.
This method uses following detection device to detect, and detection device includes vibrational system and the big portion of detecting system two
Point；The hovering drop that vibrational system is formed through capillary tube for making detection blood sample produces of reciprocating vibration, and detecting system then detects liquid
Drip the situation of change of meniscus in vibration processes, the elastic modelling quantity of blood sample can be calculated according to the situation of change of meniscus, thus
Detection coagulation.Seeing Fig. 1, Fig. 2 and Fig. 3, described capillary tube 3 and vibration generation cavity 7 is vibrational system component parts, defeathering
Outside tubule 3 and vibration generation cavity 7, vibrational system also includes sample introduction tank 1, sample presentation pipe 2, sacculus 4 and vibration power 5, at capillary tube 3
In overhang, sample introduction tank 1 is connected with capillary tube 3 by sample presentation pipe 2, to add blood sample and in capillary tube 3 times by sample introduction tank 1
The blood sample drop 6 of the band meniscus connected by surface tension is produced at the end mouth of pipe；Sample presentation pipe 2 is provided with check valve so that
Blood sample can only be flowed toward capillary tube direction by sample introduction tank and can not flow backwards；Vibration generation cavity 7 be located at check valve and capillary tube it
Between sample presentation pipe 2 on and connect with capillary tube 3, described sacculus 4 is arranged on vibration generation cavity 7, and vibration power 5 passes through driver
Structure is connected with sacculus 4 so that sacculus 4 produces reciprocating motion, and then makes blood sample drop 6 meniscus up-down vibration；Capillary tube 3 big
It is little so that Surface Tension of Liquid Drops is applied to the thrust sum of drop more than drop gravity and vibration power, so that drop is back and forth
Motion will not be dripped.
Described detecting system includes daylighting module 9 and the signal processing module that light emitting module 8 is relative with light emitting module, sends out
Optical module 8 and daylighting module 9 line are positioned at capillary tube 3 lower end vertical with capillary tube 3, and light emitting module 8 can use maturation
LED light source.Daylighting module 9 is for gathering the illumination after light emitting module passes through capillary tube lower end meniscus (by illumination variation feelings
Condition can reflect the change of meniscus) and be converted to the signal of telecommunication, daylighting module 9 output connects signal processing module, by signal
The signal of telecommunication that daylighting module is exported by reason module carries out calculating process, obtains the elastic modelling quantity of blood sample.
Described daylighting module 9 is made up of silicon cell 10 and optical fiber flexible pipe 11, and optical fiber flexible pipe 11 one end is connected to silicon photoelectricity
On pond 10, the other end is towards light emitting module 8.The light sent by light emitting module by optical fiber flexible pipe 11 imports silicon cell 10, this
The layout of sample more convenient daylighting module.Silicon cell is a kind of semiconductor device directly converting optical energy into electric energy, and technology becomes
Ripe, the situation of change of drop meniscus can be reflected easily by it.
Vibration power 5 can have multiple way of realization, as long as sacculus can be made to produce the reciprocating motion that frequency is controlled.
Implementing form as one, vibration power 5 can be air pump 12, and the piston of air pump 12 is connected with sacculus 4, air pump 12 and electricity
Magnet valve 13 connects to be controlled air pump work and work range and frequency by electromagnetic valve.
The computing formula of blood elastic modulus E is:
E=K Δ L
Wherein K is resonance amplitude difference and the elastic modelling quantity conversion coefficient of blood coagulation, by clotting assay process record
" amplitude difference-elastic modelling quantity " experiment value is obtained by linear regression analysis；Δ L is the difference of resonance amplitude before and after blood sample blood coagulation
Value, reflects the change of blood sample natural frequency before and after blood coagulation.
In Hemostasis examination, indirectly can reflect solidifying by blood sample liquid level motion amplitude in process of setting during detection vibration
Blood process.Traditional blood coagulation sample method for oscillating is that the machinery such as vibration table of blood coagulation sample Yu vibration is carried out Hard link,
Blood coagulation sample is driven to produce vibration by vibration table.The present invention changes by controlling vibration generation cavity intravascular coagulation sample liquid level pressure
Make blood coagulation sample produce vibration, as it is shown in figure 1, blood is loaded from sample introduction tank 1, arrive vibration generation cavity 7 through sample presentation pipe 2.Ball
Capsule 4 is flexible material, and balloon membrane can control sacculus by the micro air pump of connection and electromagnetic valve and move back and forth, and this athletic meeting makes to shake
Dynamic generation cavity 7 produces pressure or suction, so that blood sample produces motion and enters in capillary tube 3.Owing to blood sample is to capillary tube
Not immersional wetting (capillarity), blood sample enters can hover after capillary tube and is that, below capillary tube 3, the surface tension of blood sample makes
The meniscus of evagination is formed bottom capillary tube.Control sacculus 4 by air pump 12, electromagnetic valve 13 the most again to move with certain frequency
Making vibration generation cavity 7 produce pressure change, this pressure can make the blood sample in capillary tube also with a certain frequency vibration, and finally pass
It is delivered on the drop 6 of hovering, thus controls the up-down vibration of blood sample coagulation liquid level.Compared with the mode of vibration of vibration table, this
Bright need not designs the vibration damping of complexity, stabilization measure for vibrating device, improves stablizing of coagulation analysis instrument internal structure
Property and the precision of optical detection.
The present invention is by pressure in change capillary liquid linker so that the blood sample in capillary tube produces reciprocal fortune
Dynamic, thus cause the blood meniscus of capillary exit evagination to produce vibration.The amplitude of blood sample vibration is relevant with coagulation, blood
Solidification rate is the highest, and elastic modelling quantity is the biggest, it was observed that the motion amplitude of capillary meniscus the least.As shown in Figure 2 and Figure 3, LED
The light beam that light source sends is irradiated to the optical fiber flexible pipe on the left of meniscus on the right side of meniscus, owing to blood is opaque thickness
Liquid, the light detected on silicon cell impinges upon the intersection of meniscus and air and has a boundary signal, by detection
Meniscus is demarcated the change of signal position on silicon cell, detects the change of capillary meniscus, corresponding calculates blood
Liquid elastic modelling quantity, thus trace coagulation process.Compared with turbidimetry, testing result is affected relatively by the bubble in blood sample of the present invention
Little；Compared with substrate development process (absorbance method), present invention detection is the blood springform the most relevant to coagulation
Amount, is affected less by blood constituent；Compared with suspension method and microsphere method, higher frequency of vibration can be applied to blood sample, therefore originally
Invent the temporal resolution that coagulation is traced high.
Last it should be noted that the examples detailed above of the present invention is only for example of the present invention is described, and not
It it is the restriction to embodiments of the present invention.Although the present invention has been described in detail by applicant with reference to preferred embodiment, right
For those of ordinary skill in the field, can also make on the basis of the above description other multi-forms change and
Variation.Here cannot all of embodiment be given exhaustive.Every belong to that technical scheme amplified out aobvious and
The change that is clear to or variation are still in the row of protection scope of the present invention.
Claims (4)
1. method based on blood sample vibration detection coagulation, it is characterised in that: blood sample to be detected is added to the capillary of pendency
In pipe, to produce the blood sample drop of the band meniscus connected by surface tension at the mouth of pipe of capillary tube lower end；At capillary tube
On arrange vibration generation cavity connect with capillary tube, by the capillary tube other end close, then vibration generation cavity on apply replace
Thrust with suction so that blood sample drop meniscus produce of reciprocating vibration；Blood sample drop meniscus bottom in detection vibration processes
Change in location, and calculate the elastic modelling quantity of blood sample, thus realize coagulation detection.
Method based on blood sample vibration detection coagulation the most according to claim 1, it is characterised in that: this method uses
Following detection device detects, and detection device includes vibrational system and detecting system two large divisions；Described capillary tube and shaking
Dynamic generation cavity is vibrational system component parts, and outside defeathering tubule and vibration generation cavity, vibrational system also includes sample introduction tank, sample presentation
Pipe, sacculus and vibration power, sample introduction tank is connected with capillary tube by sample presentation pipe, to add blood sample and at capillary tube by sample introduction tank
The blood sample drop of the band meniscus connected by surface tension is produced at the mouth of pipe of lower end；Sample presentation pipe is provided with check valve so that
Blood sample can only be flowed toward capillary tube direction by sample introduction tank and can not flow backwards；Vibration generation cavity is located between check valve and capillary tube
Sample presentation pipe on and connect with capillary tube, described sacculus is arranged on vibration generation cavity, and vibration power is by drive mechanism and ball
Capsule connects so that sacculus produces reciprocating motion, and then makes blood sample drop meniscus up-down vibration；The size of capillary tube is so that drop
Surface tension is applied to the thrust sum of drop more than drop gravity and vibration power, so that drop will not in reciprocating motion
Drippage；
Described detecting system includes daylighting module and signal processing module, the light emitting module that light emitting module is relative with light emitting module
Be positioned at capillary tube lower end vertical with capillary tube with daylighting module line, the light beam that light emitting module sends is from blood sample drop meniscus
Side is irradiated in the daylighting module of meniscus opposite side, and the light detected in daylighting module impinges upon the friendship of meniscus and air
A boundary signal is had at boundary, the change of the signal position that demarcated in daylighting module by detection meniscus, and export
To signal processing module, thus detect the change of capillary meniscus, corresponding calculating blood elastic modelling quantity, thus trace blood coagulation
Process.
Method based on blood sample vibration detection coagulation the most according to claim 2, it is characterised in that: described daylighting mould
Block is made up of silicon cell and optical fiber flexible pipe, and optical fiber flexible pipe one end is connected on silicon cell, and the other end is towards light emitting module.
Method based on blood sample vibration detection coagulation the most according to claim 2, it is characterised in that: described vibration is moved
Power is air pump, and the piston of air pump is connected with sacculus, and air pump is connected with electromagnetic valve to be controlled air pump operating frequency by electromagnetic valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610560461.3A CN106248919B (en) | 2016-07-15 | 2016-07-15 | Method based on blood sample vibration detection coagulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610560461.3A CN106248919B (en) | 2016-07-15 | 2016-07-15 | Method based on blood sample vibration detection coagulation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106248919A true CN106248919A (en) | 2016-12-21 |
| CN106248919B CN106248919B (en) | 2018-11-06 |
Family
ID=57613204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610560461.3A Active CN106248919B (en) | 2016-07-15 | 2016-07-15 | Method based on blood sample vibration detection coagulation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106248919B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108663502A (en) * | 2017-03-28 | 2018-10-16 | 王英吉 | Full-automatic thrombelastogram instrument |
| CN111420858A (en) * | 2020-04-24 | 2020-07-17 | 北京森美希克玛生物科技有限公司 | A vibration excitation device |
| CN111855732A (en) * | 2019-04-28 | 2020-10-30 | 北京碧澄生物科技有限公司 | Device and method for detecting liquid phase change |
| CN113552025A (en) * | 2021-06-23 | 2021-10-26 | 安图实验仪器(郑州)有限公司 | Method for detecting blood sample resonance frequency and blood sample resonance detector |
| WO2022126845A1 (en) * | 2020-12-18 | 2022-06-23 | 重庆南方数控设备股份有限公司 | Blood viscoelasticity detection device and detection method |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1138377A (en) * | 1993-10-28 | 1996-12-18 | 伊斯塔特公司 | Apparatus for assaying viscosity changes in fluid samples and method of conducting same |
| CN1784599A (en) * | 2003-04-24 | 2006-06-07 | 希缪司构普公司 | Hemostasis analyzer and method |
| CN101308149A (en) * | 2008-07-16 | 2008-11-19 | 上海凯颐医疗仪器有限公司 | Blood clotting time measurement device and its measurement method |
| CN103403519A (en) * | 2010-09-20 | 2013-11-20 | 昆士兰大学 | Serum preparation |
| US20130344502A1 (en) * | 2011-03-15 | 2013-12-26 | Aston University | Vibrating microplate biosensing for characterising properties of behaviour biological cells |
| WO2014039909A1 (en) * | 2012-09-06 | 2014-03-13 | Theranos, Inc. | Systems, devices, and methods for bodily fluid sample collection |
| CN203825014U (en) * | 2014-04-10 | 2014-09-10 | 科拉梅德科技有限公司 | Test box for sample testing |
| CN104903728A (en) * | 2012-12-27 | 2015-09-09 | 高丽大学校产学协力团 | Apparatus and method for platelet function and drug response testing based on microfluidic chip |
| CN105004641A (en) * | 2015-08-27 | 2015-10-28 | 苏州柯尔医疗器械有限公司 | Blood analyzer and analysis method |
| CN105164528A (en) * | 2013-03-15 | 2015-12-16 | 科拉梅德科技有限公司 | Apparatus, cartridge and method for hemostasis testing |
| US20160139106A1 (en) * | 2014-11-14 | 2016-05-19 | Electronics And Telecommunications Research Institute | Apparatus and method for blood analysis |
| US20160274015A1 (en) * | 2013-08-27 | 2016-09-22 | Rheolution Inc. | System and Method for Measurements of Viscoelastic Parameters in Soft Materials |
-
2016
- 2016-07-15 CN CN201610560461.3A patent/CN106248919B/en active Active
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1138377A (en) * | 1993-10-28 | 1996-12-18 | 伊斯塔特公司 | Apparatus for assaying viscosity changes in fluid samples and method of conducting same |
| CN1784599A (en) * | 2003-04-24 | 2006-06-07 | 希缪司构普公司 | Hemostasis analyzer and method |
| CN101308149A (en) * | 2008-07-16 | 2008-11-19 | 上海凯颐医疗仪器有限公司 | Blood clotting time measurement device and its measurement method |
| CN103403519A (en) * | 2010-09-20 | 2013-11-20 | 昆士兰大学 | Serum preparation |
| US20130344502A1 (en) * | 2011-03-15 | 2013-12-26 | Aston University | Vibrating microplate biosensing for characterising properties of behaviour biological cells |
| WO2014039909A1 (en) * | 2012-09-06 | 2014-03-13 | Theranos, Inc. | Systems, devices, and methods for bodily fluid sample collection |
| CN104903728A (en) * | 2012-12-27 | 2015-09-09 | 高丽大学校产学协力团 | Apparatus and method for platelet function and drug response testing based on microfluidic chip |
| CN105164528A (en) * | 2013-03-15 | 2015-12-16 | 科拉梅德科技有限公司 | Apparatus, cartridge and method for hemostasis testing |
| US20160274015A1 (en) * | 2013-08-27 | 2016-09-22 | Rheolution Inc. | System and Method for Measurements of Viscoelastic Parameters in Soft Materials |
| CN203825014U (en) * | 2014-04-10 | 2014-09-10 | 科拉梅德科技有限公司 | Test box for sample testing |
| US20160139106A1 (en) * | 2014-11-14 | 2016-05-19 | Electronics And Telecommunications Research Institute | Apparatus and method for blood analysis |
| CN105004641A (en) * | 2015-08-27 | 2015-10-28 | 苏州柯尔医疗器械有限公司 | Blood analyzer and analysis method |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108663502A (en) * | 2017-03-28 | 2018-10-16 | 王英吉 | Full-automatic thrombelastogram instrument |
| CN108663502B (en) * | 2017-03-28 | 2023-09-08 | 常熟常江生物技术有限公司 | Full-automatic thromboelastography instrument |
| CN111855732A (en) * | 2019-04-28 | 2020-10-30 | 北京碧澄生物科技有限公司 | Device and method for detecting liquid phase change |
| CN111855732B (en) * | 2019-04-28 | 2023-07-04 | 北京碧澄生物科技有限公司 | Device and method for detecting phase change of liquid |
| CN111420858A (en) * | 2020-04-24 | 2020-07-17 | 北京森美希克玛生物科技有限公司 | A vibration excitation device |
| WO2022126845A1 (en) * | 2020-12-18 | 2022-06-23 | 重庆南方数控设备股份有限公司 | Blood viscoelasticity detection device and detection method |
| CN113552025A (en) * | 2021-06-23 | 2021-10-26 | 安图实验仪器(郑州)有限公司 | Method for detecting blood sample resonance frequency and blood sample resonance detector |
| CN113552025B (en) * | 2021-06-23 | 2024-04-16 | 安图实验仪器(郑州)有限公司 | Method for detecting resonance frequency of blood sample and blood sample resonance detector |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106248919B (en) | 2018-11-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106248919B (en) | Method based on blood sample vibration detection coagulation | |
| CN111712703B (en) | Sample analyzer and sample analysis method | |
| US10724949B2 (en) | Cuvette for detecting bacteria and determining their susceptibility to antibiotics | |
| US9378557B2 (en) | Microfluidic device for assessing object/test material interactions | |
| US5734468A (en) | Probe and method for determining serum indices of a serum sample | |
| JP2008520971A (en) | Apparatus and method for treating biological fluids | |
| JP2013533469A (en) | Methods and apparatus for detection of particle positional freedom in biological and chemical analysis and applications in immunodiagnostics | |
| CN101825640A (en) | Qualitative and quantitative detection dual-purpose HCG test paper for colloidal gold immunochromatography assay | |
| CN106226178B (en) | A kind of device using blood sample vibration detection coagulation in capillary | |
| CN201110824Y (en) | Dynamic granule image analyzer | |
| JP2015004534A (en) | Automatic analysis device and automatic analysis method | |
| KR102073662B1 (en) | Apparatus and method to determine the blood sedimentation rate and other parameters connected thereto | |
| CN108931636A (en) | Measure the fluorescence immune chromatography detection kit and preparation method thereof of ST2 | |
| CN109856410A (en) | A kind of blood clotting test device | |
| US20250172476A1 (en) | Device and method for performing a complete blood count and determining a sedimentation rate | |
| CN113504153A (en) | Blood viscosity detection device and method based on microfluidic technology | |
| CN105229472B (en) | Blood coagulation detection method | |
| JP2013053959A (en) | Coagulation activity measuring apparatus, measuring chip, and measuring method | |
| CN107576802A (en) | A kind of detection means and detection method of fluorescence micro albumen | |
| CN101251474A (en) | Dip sticky optical fiber liquid analysis method and its special analysis device | |
| JP5607593B2 (en) | Coagulation activity measuring device, measuring chip and measuring method | |
| CN212872486U (en) | Integrated instrument structure for hemagglutination analysis and blood cell counting | |
| JP7241209B2 (en) | Blood Coagulation Measuring Device, Method for Measuring Blood Coagulation Time, Method for Determining Completion of Blood Coagulation Reaction, and Automated Blood Centrifuge | |
| CN210221804U (en) | Food quality analyzer | |
| CN116337817A (en) | Blood analysis device and method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |