CN106236605A - Porous microsphere and preparation method thereof, application - Google Patents
Porous microsphere and preparation method thereof, application Download PDFInfo
- Publication number
- CN106236605A CN106236605A CN201610727949.0A CN201610727949A CN106236605A CN 106236605 A CN106236605 A CN 106236605A CN 201610727949 A CN201610727949 A CN 201610727949A CN 106236605 A CN106236605 A CN 106236605A
- Authority
- CN
- China
- Prior art keywords
- microsphere
- preparation
- plga
- porous microsphere
- porous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/85—Polyesters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0241—Containing particulates characterized by their shape and/or structure
- A61K8/025—Explicitly spheroidal or spherical shape
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0241—Containing particulates characterized by their shape and/or structure
- A61K8/0279—Porous; Hollow
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/25—Silicon; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/412—Microsized, i.e. having sizes between 0.1 and 100 microns
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to chemical field, particularly to porous microsphere and preparation method thereof, application.This porous microsphere has hierarchical porous structure, can load various active material as the carrier of active substance simultaneously, such as somatomedin, protein, many peptides etc., its stability can be increased, extend release time, maintain release concentration, reduce the pungent zest to skin simultaneously.Raw materials used for the good bio-medical material of biocompatibility, not only safety, also there is certain biological activity, can promote that cell increases, accelerate skin metabolism, be suitable as active ingredient carriers for the cosmetics of super quality.
Description
Technical field
The present invention relates to chemical field, particularly to porous microsphere and preparation method thereof, application.
Background technology
In the face of the strong market competition and consumer are to efficient, the vigorous requirement of multifunctional personal nursing materials, traditional
Design, manufacturing technology oneself can not meet the needs of the formula of the cosmetics of super quality, use existing formula designing technique to add
Content and the constituent species of the cosmetic active components added are the most extremely limited.Among various high-grade cosmetics, it is required for greatly
Have several or multiple efficacies defying age, speckle dispelling, except pox, sun-proof, uvioresistant, brighten, insulation etc..Will be in a certain cosmetics
There is several function simultaneously and do not produce negative effect in addition to adding the material with New function, it is also possible to change original component
Use and interpolation form.
Porous microsphere is a kind of material with special construction, can utilize himself bigger specific surface area and porous knot
Structure, the interpolation of change active matter and delivery mode, strengthen the stability of active substance, extend effective acting time, reduction poison pair
Effect etc..When porous microsphere is as active matter carrier, adsorbable active component therein also makes it in significant period of time
Inside being released slowly into skin surface, it is to avoid initial activity substance release is too fast using, the active substance arriving skin surface is dense
Spend height, be unfavorable for skin absorption, reach the purpose maintaining active material concentration for a long time in OK range.For zest
Active substance, can reduce it by low concentration sustained release the zest of skin is extended its action time simultaneously.Meanwhile, many
The breathability of hole microsphere and moisture retention, enable it to well and skin-compatible.
Tradition is prepared the method for porous microsphere and is mainly added the porogen such as ammonium hydrogen carbonate, sodium chloride, cyclodextrin, gathers simultaneously
Closing reaction to need to use the organic solvents such as larger amount of toluene, oxolane, material toxicity is relatively big not environmentally, may be residual in microsphere
Stay part porogen and toxic organic solvent.And the distribution of the standby porous microsphere pore structure of traditional law system is wayward.Tradition work
Skill is complicated, and cost is high, is not suitable for large-scale promotion.
Therefore it provides a kind of porous microsphere and preparation method thereof has important practical significance.
Summary of the invention
In view of this, the present invention provides a kind of porous microsphere and preparation method thereof, application.This porous microsphere has multi-stage porous
Structure, can load various active material as the carrier of active substance simultaneously, such as somatomedin, protein, many peptides etc.,
Its stability can be increased, extend release time, maintain release concentration, reduce the pungent zest to skin simultaneously.Institute
It is the good bio-medical material of biocompatibility with raw material, not only safety, also there is certain biological activity, cell can be promoted
Increase, accelerate skin metabolism, be suitable as active ingredient carriers for the cosmetics of super quality.
In order to realize foregoing invention purpose, the present invention provides techniques below scheme:
The invention provides a kind of porous microsphere, its raw material does not include porogen and toxic organic solvent；Described porous is micro-
Ball, with PLGA, calcium silicates, mesoporous silicon and multiple emulsion stabilizer as raw material, utilizes inorganic component calcium silicates and mesoporous silicon at microsphere
Surface and internal in-situ pore-forming.
In some specific embodiments of the present invention, described calcium silicates is 1/20～1/2 with the mass ratio of described PLGA.
In some specific embodiments of the present invention, described mesoporous silicon is 1/20～1/2 with the mass ratio of described PLGA.
In some specific embodiments of the present invention, described multiple emulsion stabilizer is 1 with the mass ratio of described PLGA
～3.
Present invention also offers the preparation method of described porous microsphere, comprise the steps:
Calcium silicates after step 1: PLGA is dissolved in organic solvent, with pulverizing, the mesoporous silicon after pulverizing and oil-soluble
Active component mixes, and stirring also ultrasonic is uniformly dispersed to inorganic powder, obtains oil phase；
Step 2: take multiple emulsion stabilizer and mix with water, the aqueous solution of preparation polyvinyl alcohol；Again with water-soluble active thing
Matter mixes, and is uniformly mixing to obtain aqueous phase；
Step 3: oil phase is mixed with water, and it is stirred continuously until organic solvent volatilization completely, obtain the compound of solidification
Microsphere；
Step 4: take that step 3 prepares complex microsphere is scrubbed, lyophilization, obtain described porous microsphere.
In some specific embodiments of the present invention, calcium silicates described in described preparation method and the quality of described PLGA
Ratio is 1/20～1/2；
Described mesoporous silicon is 1/20～1/2 with the mass ratio of described PLGA；
Described multiple emulsion stabilizer is 1～3 with the mass ratio of described PLGA；
In terms of g/mL, described PLGA is 1/20～1/5 with the mass volume ratio of described organic solvent.
In some specific embodiments of the present invention, in described preparation method,
Described organic solvent includes a kind of or both above the mixing in acetone, dichloromethane, chloroform, oxolane
Compound；
In terms of g/mL, multiple emulsion stabilizer described in step 2 is 1/500～1/100 with the mass volume ratio of water.
In some specific embodiments of the present invention, described multiple emulsion stabilizer is polyvinyl alcohol.
In some specific embodiments of the present invention, in described preparation method, content meter by mass percentage, described oil
The addition of soluble active ingredient accounts for the 0.1%～5% of oil phase；
Described oil soluble active ingredients includes glycyrrhizin, coenzyme Q10, bisabolol, vitamin E, lecithin, ascorbic acid
A kind of or both mixture above in ester, ceramide.
In some specific embodiments of the present invention, in described preparation method, content meter by mass percentage, described water
The addition of soluble active substance accounts for the 0.1%-5% of aqueous phase；
Described water-soluble actives include epidermal growth factor, collagen protein, cytokine, Punica granatum L. extract,
A kind of or both mixture above in Pseudoalteromonas tunning extract, Ramulus Sambuci Williamsii extract, acetyl group tetrapeptide.
In some specific embodiments of the present invention, in described preparation method, power ultrasonic described in step 1 is
250～350W, the described ultrasonic time is 15～30min；
Described in step 1, the speed of stirring is 200～600rpm, and the time of described stirring is 4～10h；
Described in step 3, the speed of stirring is 200～600rpm, and the time of described stirring is 8～16h；
Cryodesiccated temperature described in step 4 is-50 DEG C～-40 DEG C, and the described cryodesiccated time is 24～48h.
Present invention also offers described porous microsphere or the porous microsphere prepared according to described preparation method is being made up
Application in product.
Porous microsphere in the present invention uses the biomaterials such as PLGA, calcium silicates and mesoporous silicon to be raw material, molten by emulsion
Agent volatility process is prepared from.The present invention prepares the method for porous microsphere without any porogen, utilizes calcium silicates and mesoporous silicon
Deng inorganic component at the surface of microsphere and internal in-situ pore-forming, the porous forming a large amount of micropore in surface and internal cavity structures is micro-
Ball.The porous microsphere of the present invention can be wrapped in microsphere inside as the carrier of various active composition and passed through surface micropore
Slowly release, extends the action time of active component, has good slow release effect.This porous microsphere is not added with any pore
Agent, avirulence porogen component residue problem.The raw material of preparing of this porous microsphere is bio-medical material, has good life
The thing compatibility, can promote cell proliferation, repairs impaired cell tissue, accelerates skin metabolism, is that one is applicable to top grade
The novel carriers of cosmetic field, has the most wide application prospect.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
In having technology to describe, the required accompanying drawing used is briefly described.
Fig. 1 shows the preparation process schematic diagram of specific embodiment of the invention porous microsphere；
Fig. 2 shows the scanning electron microscope (SEM) photograph of microsphere surface in the embodiment of the present invention 1；
Fig. 3 shows the scanning electron microscope (SEM) photograph of microsphere internal cross section in the embodiment of the present invention 1；
Fig. 4 shows the scanning electron microscope (SEM) photograph of microsphere surface in the embodiment of the present invention 2；
Fig. 5 shows the scanning electron microscope (SEM) photograph of microsphere internal cross section in the embodiment of the present invention 2；
Fig. 6 shows the releasing curve diagram of embodiment of the present invention 1-3；
Fig. 7 shows dermal fibroblasts proliferative conditions in the embodiment of the present invention 1 on porous microsphere.
Detailed description of the invention
The invention discloses a kind of porous microsphere and preparation method thereof, application, those skilled in the art can use for reference herein
Content, is suitably modified technological parameter and realizes.Special needs to be pointed out is, all similar replacements and change are to people in the art
Being apparent from for Yuan, they are considered as being included in the present invention.The method of the present invention and application have been passed through the most real
Executing example to be described, related personnel substantially can be to method described herein in without departing from present invention, spirit and scope
It is modified with application or suitably changes and combine, realize and apply the technology of the present invention.
The purpose of the present invention can be achieved through the following technical solutions:
Can be used for the porous microsphere of the cosmetics of super quality, it is characterised in that with bio-medicals such as PLGA, calcium silicates and mesoporous silicons
Material is raw material, utilizes the inorganic component such as calcium silicates and mesoporous silicon at the surface of microsphere and internal in-situ pore-forming, and microsphere surface is equal
Even being dispersed with a large amount of micropore, inside is cavity structure, and active component is wrapped in internal cavities and the surface hole defect of microsphere.
Can be used for the preparation method of the porous microsphere of the cosmetics of super quality, comprise the following steps:
1. PLGA is dissolved in organic solvent, adds calcium silicates powder, mesoporous silicon powder and oil soluble active ingredients, stir
Mix and ultrasonic be uniformly dispersed to inorganic powder, obtaining oil phase；
2. prepare the aqueous solution of polyvinyl alcohol, add water-soluble actives, be uniformly mixing to obtain aqueous phase；
3. under conditions of stirring, oil phase is slowly added in aqueous phase, and is stirred continuously until dichloromethane volatilization completely,
Obtain the complex microsphere of solidification；
4. collecting the microsphere in step 3, be washed with deionized 5 times, lyophilization i.e. obtains described porous microsphere.
Described organic solvent includes a kind of or both above the mixing in acetone, dichloromethane, chloroform, oxolane
Compound.
Content meter by mass percentage, oil soluble active ingredients addition can account for the 0.1%-5% of oil phase
Content meter by mass percentage, the addition of water-soluble actives accounts for the 0.1%-5% of aqueous phase.
In said method, in described step 1, oil soluble active ingredients includes: glycyrrhizin, coenzyme Q10, bisabolol, dimension
One or more in raw element E, lecithin, acid ascorbyl ester, ceramide.
In said method, in described step 2, water-soluble actives includes: epidermal growth factor, collagen protein, cell
In activin, Punica granatum L. extract, Pseudoalteromonas tunning extract, Ramulus Sambuci Williamsii extract, acetyl group tetrapeptide one
Plant or several.
In said method, in described step 1, PLGA is 1/20～1/5g/ml with the mass volume ratio of organic solvent；Silicic acid
Calcium powder is 1/20～1/2 with the mass ratio of PLGA；Mesoporous silicon is 1/20～1/2 with the mass ratio of PLGA.In said method, institute
Stating in step 2, polyvinyl alcohol is 1/500～1/100g/ml with the mass volume ratio of water.
In said method, in described step 1, ultrasonic power is 250～350W, and ultrasonic time is 15～30 minutes.
In said method, in described step 1, stir speed (S.S.) is 200～600rpm, and mixing time is 4～10 hours.
In said method, in described step 3, stir speed (S.S.) is 200～600rpm, and mixing time is 8～16 hours.
In said method, in described step 4, lyophilization temperature is-50 DEG C～-40 DEG C, sublimation drying be 24～
48 hours.
Without any porogen in the preparation method of the porous microsphere that the present invention provides, utilize calcium silicates and mesoporous silicon etc.
Inorganic constituents is in-situ pore-formed on PLGA microsphere, prepares porous microsphere.This porous microsphere surface is uniform-distribution with a large amount of micropore, interior
Portion is cavity.Microsphere surface and internal holes size and number all can regulate and control by changing preparation technology parameter.By PLGA/ silicic acid
Calcium/mesoporous silicon composite porous microspheres is applied to cosmetic field as the carrier of various active composition, and raw material used is all made a living
Thing medical material, has good biocompatibility.Microsphere exists the mesoporous of internal macropore, surface micropore and mesoporous silicon simultaneously,
It it is the complex microsphere of a kind of hierarchical porous structure.
Have the beneficial effect that
1. the porous microsphere appearance structure of preparation is controlled, regulates the porous pattern of microsphere by changing preparation technology parameter
Structure；
2. this porous microsphere can load oil-soluble active, also can load water-soluble actives, moreover it is possible to same to fashionable dress
Carry oil-soluble and water-soluble actives, be advantageously implemented pleiotropy and the high efficiency of cosmetics；
3. this porous microsphere can significantly improve the release time of active substance, reduces the prominent property released, maintains the concentration of active matter
In OK range, and strengthen the stability of active substance；
4. this porous microsphere can be coated with pungent, it is to avoid with direct skin contact, reduces the stimulation to skin
Property；
5. this porous microsphere uses the biomaterial that biocompatibility is good to prepare, not only safety non-toxic, moreover it is possible to promote thin
Born of the same parents breed, and accelerate skin metabolism, repair damaged cell.
In porous microsphere and preparation method thereof that the present invention provides, application, raw materials used and reagent all can be buied by market.
Below in conjunction with embodiment, the present invention it is expanded on further:
Embodiment 1 carries the preparation of the porous complex microsphere of epidermal growth factor
1g PLGA is dissolved in 10ml dichloromethane, adds 0.2g calcium silicates powder and 0.2g mesoporous silicon powder, 200rpm
Within after stirring 6 hours ultrasonic 15 minutes, it is uniformly dispersed to inorganic powder, obtains oil phase；
Weigh 2g polyvinyl alcohol in 300ml deionized water, prepare the poly-vinyl alcohol solution of 0.67% (w/v), be subsequently adding
3g epidermal growth factor, is uniformly mixing to obtain aqueous phase；
Under the mixing speed of 200rpm, oil phase is slowly added dropwise to aqueous phase, and continuously stirred 12 hours until dichloro
Methane volatilization completely, obtains the complex microsphere of solidification；
Collect complex microsphere, be washed with deionized 5 times ,-40 DEG C of lyophilizations 48 hours, obtain carrying epidermal growth factor
Porous complex microsphere.
Embodiment 2 carries the preparation of the porous complex microsphere of coenzyme Q10
1g PLGA is dissolved in 5ml dichloromethane, adds 0.1g calcium silicates powder, 0.1g mesoporous silicon powder and 50mg auxiliary
Enzyme Q10,300rpm are uniformly dispersed after stirring 4 hours for ultrasonic 30 minutes to inorganic powder, obtain oil phase；
Weigh 3g polyvinyl alcohol in 300ml deionized water, prepare the polyvinyl alcohol water solution of 1% (w/v), stir
Obtain aqueous phase；
Under the mixing speed of 300rpm, oil phase is slowly added dropwise to aqueous phase, and continuously stirred 8 hours until dichloromethane
Alkane volatilization completely, obtains the complex microsphere of solidification；
Collect complex microsphere, be washed with deionized 5 times ,-45 DEG C of lyophilizations 24 hours, obtain carrying coenzyme Q10 many
Hole complex microsphere.
Embodiment 3 carries the preparation of the porous complex microsphere of acid ascorbyl ester and cytokine
1g PLGA is dissolved in 20ml chloroform, adds 0.05g calcium silicates powder, 0.05g mesoporous silicon powder and 1g and resist
Bad hematic acid ester, 400rpm is uniformly dispersed to inorganic powder after stirring 8 hours, obtains oil phase for ultrasonic 20 minutes；
Weigh 2g polyvinyl alcohol in 500ml deionized water, prepare the poly-vinyl alcohol solution of 0.4% (w/v), be subsequently adding
5g cytokine, is uniformly mixing to obtain aqueous phase；
Under the mixing speed of 400rpm, oil phase is slowly added dropwise to aqueous phase, and continuously stirred 16 hours until trichlorine
Methane volatilization completely, obtains the complex microsphere of solidification；
Collect complex microsphere, be washed with deionized 5 times ,-50 DEG C of lyophilizations 36 hours, carried ascorbic acid simultaneously
The porous complex microsphere of ester and cytokine.
Embodiment 4 carries the porous microsphere extracorporeal releasing experiment of active component
The porous microsphere that 2mg carries active component adds in 10ml PBS, seals and is placed on 37 DEG C, 90rpm
Shaking table vibrates, at set intervals, utilizes ultraviolet spectrophotometer to measure the concentration of active component in supernatant, according to input
The volume of amount and supernatant calculates the percentage ratio of drug release.Overall length was 24 hours, according to time and cumulative release release time
Percentage ratio obtains active component releasing curve diagram.Refer to table 1, Fig. 6.
Table 1 release profiles initial data
Result is as shown in Figure 6, it can be seen that compared with blank group, and porous microsphere delays as the active component of carrier
Time of releasing significantly extends (P ＜ 0.05), and overall sustained release is effective.
The in-vitro multiplication experiment on porous microsphere prepared by the present invention of embodiment 5 dermal fibroblasts
With embodiment 1 preparation carry epidermal growth factor porous complex microsphere as experimental group, be not added with epidermal growth factor
The porous complex microsphere of son is matched group, compares dermal fibroblasts proliferative conditions on two kinds of microspheres.By two kinds of microspheres
Respectively with 75% alcohol solution dipping 2 hours, and irradiate 30 minutes sterilizations under ultraviolet light；Then 5 are washed with PBS
Time, soak microsphere 4 hours by culture medium；Suck culture medium, add 100 μ l/ hole (1 × 106cells/ml) cell suspension, transfer
Within 4 hours to incubator, treating that most cells sticks on support, then every hole supplements 400 μ l culture medium, puts into 37 DEG C of dioxies
Change in carbon incubator and cultivate；Cell proliferative conditions on microsphere is detected in the time point set CCK-8 method.
CCK-8 method:
(1) CCK-8 standard curve is made: with the cell quantity in the cell suspension prepared by cell counting count board calculating；So
It is diluted to a cell concentration the most successively by culture medium afterwards and plants in 96 orifice plates, general by 3-5 Concentraton gradient, often group
3-6 Duplicate Samples；Cultivate after inoculation and make cell attachment in 4 hours, then add CCK-8 reagent and cultivate in incubator 1 hour；Cultivate
After base colour developing, slight oscillatory makes culture medium mix, and then measures the OD value in each hole at 450nm wavelength by microplate reader, makes one
Bar with cell as abscissa (X-axis), O.D value as vertical coordinate the standard curve of (Y-axis).In the case of experiment condition is consistent, mark
The incubation time after determining the inoculation quantity of cell and adding CCK-8 is easy in the making of directrix curve.
(2) in predetermined point of time, CCK-8 stock solution culture medium is obtained working solution by 1/10 (v/v) dilution, draws orifice plate
In culture medium, add 100 μ l/ hole working solutions, be then placed in incubator lucifuge cultivate 1 hour, by microplate reader at 450nm wavelength
Place measures the absorption value in each hole, is mixed by orifice plate slight oscillatory before test.
The results are shown in Table 2, Fig. 7.
The initial data of table 2 proliferative conditions
From table 2 result, the porous microsphere of embodiment 1 preparation is compared with matched group, and absorption value significantly improves (P ＜
0.05), therefore, the porous microsphere that the present invention provides can promote cell proliferation, accelerates skin metabolism, is suitable as activity
Material carrier is used for the cosmetics of super quality.
The porous microsphere of Example 2, embodiment 3 preparation carries out above-mentioned test, and result of the test is many with embodiment 1 preparation
The cultivation effect of hole microsphere is close, the increasing of the porous microsphere that the porous microsphere of embodiment 2, embodiment 3 preparation is prepared with embodiment 1
Grow effect without significant difference (P ＞ 0.05).
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For Yuan, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a porous microsphere, it is characterised in that its raw material does not include porogen and toxic organic solvent；Described porous microsphere with
PLGA, calcium silicates, mesoporous silicon and multiple emulsion stabilizer are raw material, utilize inorganic component calcium silicates and mesoporous silicon at microsphere surface
With internal in-situ pore-forming.
Porous microsphere the most according to claim 1, it is characterised in that described calcium silicates is 1/ with the mass ratio of described PLGA
20～1/2.
Porous microsphere the most according to claim 1 and 2, it is characterised in that described mesoporous silicon and the mass ratio of described PLGA
It is 1/20～1/2.
4. according to the porous microsphere described in any one of claims 1 to 3, it is characterised in that described multiple emulsion stabilizer and institute
The mass ratio stating PLGA is 1～3.
5. according to the preparation method of the porous microsphere described in any one of Claims 1-4, it is characterised in that comprise the steps:
Calcium silicates after step 1: PLGA is dissolved in organic solvent, with pulverizing, the mesoporous silicon after pulverizing and oil-soluble active
Composition mixes, and stirring also ultrasonic is uniformly dispersed to inorganic powder, obtains oil phase；
Step 2: take multiple emulsion stabilizer and mix with water, the aqueous solution of preparation polyvinyl alcohol；Mix with water-soluble actives again
Close, be uniformly mixing to obtain aqueous phase；
Step 3: oil phase is mixed with water, and it is stirred continuously until organic solvent volatilization completely, obtain the complex microsphere of solidification；
Step 4: take that step 3 prepares complex microsphere is scrubbed, lyophilization, obtain described porous microsphere.
Preparation method the most according to claim 5, it is characterised in that described calcium silicates is 1/ with the mass ratio of described PLGA
20～1/2；
Described mesoporous silicon is 1/20～1/2 with the mass ratio of described PLGA；
Described multiple emulsion stabilizer is 1～3 with the mass ratio of described PLGA；
In terms of g/mL, described PLGA is 1/20～1/5 with the mass volume ratio of described organic solvent.
7. according to the preparation method described in claim 5 or 6, it is characterised in that
Described multiple emulsion stabilizer is polyvinyl alcohol；
Described organic solvent includes a kind of or both mixing above in acetone, dichloromethane, chloroform, oxolane
Thing；
In terms of g/mL, multiple emulsion stabilizer described in step 2 is 1/500～1/100 with the mass volume ratio of water.
8. according to the preparation method described in any one of claim 5 to 7, it is characterised in that content meter by mass percentage, described
The addition of oil soluble active ingredients accounts for the 0.1%～5% of oil phase；
Described oil soluble active ingredients include glycyrrhizin, coenzyme Q10, bisabolol, vitamin E, lecithin, acid ascorbyl ester,
A kind of or both mixture above in ceramide；
Content meter by mass percentage, the addition of described water-soluble actives accounts for the 0.1%-5% of aqueous phase；
Described water-soluble actives includes epidermal growth factor, collagen protein, cytokine, Punica granatum L. extract, false friendship
For a kind of or both mixture above in Zymomonas mobilis tunning extract, Ramulus Sambuci Williamsii extract, acetyl group tetrapeptide.
9. according to the preparation method described in any one of claim 5 to 8, it is characterised in that power ultrasonic described in step 1 is
250～350W, the described ultrasonic time is 15～30min；
Described in step 1, the speed of stirring is 200～600rpm, and the time of described stirring is 4～10h；
Described in step 3, the speed of stirring is 200～600rpm, and the time of described stirring is 8～16h；
Cryodesiccated temperature described in step 4 is-50 DEG C～-40 DEG C, and the described cryodesiccated time is 24～48h.
10. according to the porous microsphere described in any one of Claims 1-4 or according to the preparation described in any one of claim 5 to 9
The porous microsphere that method prepares application in cosmetics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610727949.0A CN106236605A (en) | 2016-08-25 | 2016-08-25 | Porous microsphere and preparation method thereof, application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610727949.0A CN106236605A (en) | 2016-08-25 | 2016-08-25 | Porous microsphere and preparation method thereof, application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106236605A true CN106236605A (en) | 2016-12-21 |
Family
ID=57596284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610727949.0A Pending CN106236605A (en) | 2016-08-25 | 2016-08-25 | Porous microsphere and preparation method thereof, application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106236605A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107281088A (en) * | 2017-07-13 | 2017-10-24 | 成都新柯力化工科技有限公司 | A kind of Rice oil face cream and preparation method thereof |
| CN108113975A (en) * | 2018-02-02 | 2018-06-05 | 中国人民解放军军事科学院军事医学研究院 | It is a kind of based on the PLGA method for preparing microsphere of vortex oscillator and its application |
| CN108308176A (en) * | 2018-01-12 | 2018-07-24 | 中国建筑材料科学研究总院有限公司 | Diatom soil matrix plants antimicrobial composite material and preparation method |
| CN108653817A (en) * | 2018-05-24 | 2018-10-16 | 上海其胜生物制剂有限公司 | A kind of preparation and application of novel collagen stimulant |
| CN109875930A (en) * | 2019-03-11 | 2019-06-14 | 华南理工大学 | A kind of essence for skin crow's feet repair active ingredient coating and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130266625A1 (en) * | 2011-01-24 | 2013-10-10 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Nanoparticles based on poly (lactic glycolic) acid for cosmetic applications |
| CN105031718A (en) * | 2015-08-27 | 2015-11-11 | 华南理工大学 | Bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and preparation method thereof |
-
2016
- 2016-08-25 CN CN201610727949.0A patent/CN106236605A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130266625A1 (en) * | 2011-01-24 | 2013-10-10 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Nanoparticles based on poly (lactic glycolic) acid for cosmetic applications |
| CN105031718A (en) * | 2015-08-27 | 2015-11-11 | 华南理工大学 | Bone repair porous compound scaffold based on 3D (three-dimensional)-Bioplotter printing technology and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| ZENGJIANG WEI ET AL: "Facile fabrication of biocompatible PLGA drug-carrying microspheres by O/W pickering emulsions", 《COLLOIDS AND SURFACES B: BIOINTERFACES》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107281088A (en) * | 2017-07-13 | 2017-10-24 | 成都新柯力化工科技有限公司 | A kind of Rice oil face cream and preparation method thereof |
| CN108308176A (en) * | 2018-01-12 | 2018-07-24 | 中国建筑材料科学研究总院有限公司 | Diatom soil matrix plants antimicrobial composite material and preparation method |
| CN108113975A (en) * | 2018-02-02 | 2018-06-05 | 中国人民解放军军事科学院军事医学研究院 | It is a kind of based on the PLGA method for preparing microsphere of vortex oscillator and its application |
| CN108653817A (en) * | 2018-05-24 | 2018-10-16 | 上海其胜生物制剂有限公司 | A kind of preparation and application of novel collagen stimulant |
| CN108653817B (en) * | 2018-05-24 | 2021-02-02 | 上海其胜生物制剂有限公司 | Preparation method of novel collagen stimulant |
| CN109875930A (en) * | 2019-03-11 | 2019-06-14 | 华南理工大学 | A kind of essence for skin crow's feet repair active ingredient coating and preparation method thereof |
| CN109875930B (en) * | 2019-03-11 | 2021-09-21 | 华南理工大学 | Essence coated with active ingredients for repairing skin crow's feet and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106236605A (en) | Porous microsphere and preparation method thereof, application | |
| Basavalingappa et al. | Expanding the functional scope of the fmoc‐diphenylalanine hydrogelator by introducing a rigidifying and chemically active urea backbone modification | |
| CN102172498B (en) | Three-dimensional porous chitosan/gelatin microsphere, preparation method thereof and application thereof in hepatocyte culture | |
| CN106491644A (en) | A kind of fullerene soft capsule and preparation method | |
| CN104622710A (en) | Preparation and application of salicylic acid and chitosan compound composition | |
| CN109316439A (en) | A kind of suspension maintenance Essence containing Crithmum maritimum stem cell | |
| CN104983590B (en) | A kind of nano liposomes powder of Chitosan Coating and preparation method thereof | |
| CN107088183B (en) | A kind of cosmetic material and preparation method thereof | |
| Liu et al. | Co3O4/carbon nanohybrids embedded in the fibrous scaffolds promote stem cell osteogenic differentiation via strengthening cell mechanotransduction | |
| CN109010885A (en) | A kind of essence emulsion gel composition and its preparation and application | |
| Gong et al. | Injectable foam-like scaffolds release glucose oxidase-integrated metal–organic framework hybrids for diabetic bone defects | |
| CN103993060A (en) | Preparation method for controllable silk fibroin particle | |
| CN103520102A (en) | Vitamin D nano-emulsion and preparation method thereof | |
| CN103520103A (en) | Vitamin E nano-emulsion and preparation method thereof | |
| CN102585276B (en) | Method for preparing porous chitosan scaffold | |
| CN109052650A (en) | A kind of preparation of immobilized microalgae water quality control agent | |
| TW201417827A (en) | Bacteriorhodopsin-contained bioactive gel and method for preparing same | |
| CN106256423A (en) | A kind of heat resistant type Herba Salviae Japonicae essential oil microcapsule and preparation method thereof | |
| CN101878777B (en) | Chitosan-embedding white-ant drug, preparation method and application thereof | |
| CN104622805A (en) | Econazole nitrate microemulsion and preparation method and application thereof | |
| Zhu et al. | Surface modification of hydroxyapatite microspheres for the sustained release of vitamin C | |
| CN105193826B (en) | Preparation method of vitamin D3 premix and prepared vitamin D3 premix | |
| CN113679050B (en) | Fat-soluble tea polyphenol copper assembled nanoflower controlled release carrier and preparation method thereof | |
| Wei et al. | Composite Graphene for the Dimension-and Pore-Size-Mediated Stem Cell Differentiation to Bone Regenerative Medicine | |
| CN109010914A (en) | A kind of stem cell bracket and preparation method thereof applied to iron doping region |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161221 |