CN106215221A - Oligochitosan gelatin akermanite nanofiber biological dressing and preparation method thereof - Google Patents

Abstract

The invention discloses a chitosan-gelatin-magnesia feldspar nanofiber biological dressing and a preparation method thereof. Specifically, the dressing is prepared by an electrospinning method from a gel suspension comprising chitosan oligosaccharide, gelatin, feldspar and solvent. The composite nanofiber biological dressing of the present invention overcomes the disadvantages of a single dressing component, and is more suitable for the biological diversity and complexity of skin tissue. It not only ensures that the wound is in a humid environment, but also helps to accelerate the formation of blood vessels and promote the formation of granulation tissue. It also maintains the constant temperature of the wound surface, which is conducive to the healing of tissues without scabs, and has good moisture retention, air permeability, bacteriostasis, and biocompatibility, and is easy to operate and use.

Description

Oligochitosan-gelatin-magnesia feldspar nanofiber biological dressing and preparation method thereof

technical field

The invention belongs to the technical field of medical materials, and relates to a composite nanofiber biological dressing containing chitosan oligosaccharide, gelatin and feldspar and a preparation method thereof.

Background technique

In recent years, research on chitin and chitosan (chitosan) has been widely carried out at home and abroad, and their functions in anti-tumor, preparation of wound dressings, and prevention and treatment of pathogenic microorganisms have been affirmed. But because it is insoluble in water, its development and application are greatly limited. After degrading chitosan into chitooligosaccharides (also known as oligoglucosamine and chitooligosaccharides, the degree of polymerization is usually between 2 and 20) by appropriate methods, it is found that it is not only good in water solubility, but also easily absorbed by the human body. It has high bioavailability, and has various physiological functions such as antibacterial, antitumor, blood lipid regulation, immune regulation, and wound healing promotion. Studies have found that chitosan has the functions of hemostasis, strengthening inflammation and repairing cells, thereby accelerating wound healing, and using it as a wound dressing can obtain a more direct therapeutic effect.

Gelatin is a product of partial hydrolysis of collagen. Its amino acid composition is similar to that of collagen, and it has good biocompatibility. Chemical properties, can be used as hemostatic sponges, dressings, drug carriers, gelatin microfibers can simulate the extracellular matrix structure of various tissues and organs of the human body, and are used in biomedicine, tissue engineering and other fields.

Magnesite feldspar (Ca ₂ MgSi ₂ O ₇ ) is a calcium-magnesium-silicon-containing bioactive ceramic with sufficient mechanical strength, good biocompatibility, suitable degradation rate and three-dimensional porous structure. It has been reported. Magnesite feldspar forms an osseointegration with living tissue through a calcium-phosphorus layer similar to hydroxyapatite, which prevents the formation of fibrous capsules and reduces the possibility of tissue repair failure.

At present, there is an increasing demand for wound dressings clinically (such as burns, external trauma, long-term non-healing ulcers, post-operative wound care, etc.), while traditional dressings are gradually becoming airtight and easy to damage when changing dressings. Disadvantages such as new granulation tissue and poor physical sensation of patients. Modern medicine advocates moist healing, and believes that a moist environment is more likely to promote wound healing. The reason is that moist healing accelerates the formation of blood vessels and promotes the formation of granulation tissue. The new granulation tissue will suffer mechanical injury again, and the pain will be relieved.

Due to the biodiversity and complexity of skin tissue, single-component dressings are far from meeting the needs of skin tissue repair. Therefore, it is urgent to develop a dressing made of composite materials to meet related needs.

Contents of the invention

In view of the above situation, the object of the present invention is to provide a kind of oligochitosan-gelatin-magnesia feldspar nanofiber biological dressing and its preparation method. The present invention uses chitosan oligosaccharide, gelatin and feldspar in combination, and utilizes electrospinning technology to make nanofiber wet dressing from the above three components. It can promote angiogenesis and tissue regeneration, is easy to operate, has obvious clinical effects, and plays an important role in the field of wound repair.

In order to achieve the above object, the present invention adopts following technical scheme:

A chitosan oligosaccharide-gelatin-magnesia feldspar nanofiber biological dressing is prepared from a gel suspension comprising chitosan oligosaccharide, gelatin, magnesia feldspar and a solvent through an electrospinning method.

Preferably, in the above-mentioned biological dressing, the mass concentration of chitosan oligosaccharide in the gel suspension is 0.1-5 g/100 mL, preferably 0.3-3 g/100 mL.

Preferably, in the above-mentioned biological dressing, the oligochitosan is a degradation product of chitosan, with a molecular weight of 1000-2500 Da and a degree of polymerization of 2-8; the degradation is accomplished by physical, chemical or enzymatic methods.

Preferably, in the above-mentioned biological dressing, the mass concentration of gelatin in the gel suspension is 2-15g/100mL, preferably 4-14g/100mL.

Preferably, in the above-mentioned biological dressing, the mass concentration of feldspar in the gel suspension is 1-20 g/100 mL, preferably 1.5-10 g/100 mL.

Preferably, in the above-mentioned biological dressing, the solvent is selected from any one of water, glacial acetic acid, formic acid, 2,2,2-trifluoroethanol (TFE) or a mixture thereof in any proportion.

The preparation method of above-mentioned oligochitosan-gelatin-magnesia feldspar nanofiber biological dressing, it comprises the following steps:

1) Weighing chitosan oligosaccharide and gelatin, adding them to a solvent to swell overnight, and stirring at 45-60°C for 0.5-2 hours to obtain a gel-like mixture;

2) Grinding feldspar until the particle size is less than 10 μm, collecting dry feldspar powder, adding it to the gel-like mixture obtained in step 1), and stirring at 45-60° C. for 1-3 hours to obtain a gel suspension;

3) By electrospinning, the gel suspension obtained in step 2) is made into nanofibers with a diameter of 100-1000nm, and flattened to form a fiber mat to obtain chitooligosaccharide-gelatin-magnesia feldspar nanofiber biological dressing.

Preferably, in the above preparation method, the grinding in step 2) is done by using a ball mill, dry grinding 4 to 6 times, each time for 2 to 8 minutes, and the rotation speed is 700 to 1200 rpm.

Preferably, in the above preparation method, the electrospinning parameters described in step 3) are as follows: the inner diameter of the spinneret is 0.6mm, the injection flow rate is 0.2-1.5mL/h, the electric field strength is 5-20kV/cm, and the electric field strength is 5-20kV/cm. The spinning distance is 10-20cm.

Compared with the prior art, the present invention adopting the above-mentioned technical solution has the following advantages:

1. The composite nanofiber biological dressing of the present invention overcomes the disadvantages of using a single dressing component in the prior art, and can better adapt to the biodiversity and complexity of skin tissue;

2. The composite nanofiber biological dressing of the present invention belongs to the category of moist dressings, which not only ensures that the wound is in a moist environment, but also helps to accelerate the formation of blood vessels, promote the formation of granulation tissue, and maintain the constant temperature of the wound surface, which is conducive to the non-toxicity of tissues. The scab heals, preventing the new granulation tissue from suffering mechanical damage again;

3. The composite nanofiber biological dressing of the present invention has good moisture retention, air permeability, bacteriostasis and biocompatibility, and is easy to operate and use.

detailed description

The present invention will be further described in detail through specific examples below, but it should be understood that the following examples are not intended to limit the protection scope of the present invention. In addition, unless otherwise specified, the instruments, materials, reagents, etc. used in the following examples can be obtained through conventional commercial means.

Example 1: Preparation of oligochitosan-gelatin-magnesia feldspar nanofiber biological dressing.

(1) Weigh 0.75g of chitosan oligosaccharide (molecular weight is 1000-2500Da, degree of polymerization is 2-8, obtained by chemical degradation of chitosan) and 7.5g of gelatin, add to 100mL of 2% acetic acid aqueous solution Swell in medium overnight, stir at 45°C for 2 hours to obtain a transparent, viscous gel-like mixture;

(2) Use a ball mill to grind 2 g of feldspar feldspar, dry grinding 4 times, each time for 8 minutes, and the rotation speed is 1200 rpm, until the particle size of feldspar feldspar is less than 10 μm, collect the dry powder, add it to the above gel-like mixture, and dry it at 45 ° C Stir for 3h to obtain a uniform gel suspension;

(3) After the above-mentioned gel suspension is extruded by a syringe pump at a constant speed, it is forced to be sprayed and stretched to a collection device (fiber diameter is 500nm) by a high-voltage electric field force, and flattened to form a fiber mat to obtain chitosan oligosaccharides - Gelatin-magnesia feldspar nanofiber biological dressing, wherein: the inner diameter of the spinneret used for electrospinning is 0.6mm, the injection flow rate is 0.5mL/h, the electric field strength is 10kV/cm, and the electrospinning distance is 12cm.

Example 2: Preparation of oligochitosan-gelatin-magnesia feldspar nanofiber biological dressing.

(1) Weigh 1g of chitosan oligosaccharide (molecular weight is 1000～2500Da, degree of polymerization is 2～8, obtained by enzymatic degradation of chitosan) and 13g of gelatin, add to 100mL of deionized water to swell overnight, Stir at 60°C for 1 h to obtain a transparent, viscous gel-like mixture;

(2) Use a ball mill to grind 5g of feldspar feldspar, dry grinding 6 times, each grinding 6min, rotating speed 700rpm, until the particle size of feldspar feldspar is less than 10μm, collect the dry powder, add it to the above gel mixture, at 60 ℃ Stir for 1h to obtain a uniform gel suspension;

(3) After the above-mentioned gel suspension is extruded at a constant speed by a syringe pump, it is forced to be sprayed and stretched to a collection device (fiber diameter is 800nm) by a high-voltage electric field force, and flattened to form a fiber mat to obtain chitosan oligosaccharides - Gelatin-magnesia feldspar nanofiber biological dressing, wherein: the inner diameter of the spinneret used for electrospinning is 0.6mm, the injection flow rate is 1mL/h, the electric field strength is 15kV/cm, and the electrospinning distance is 18cm.

Example 3: Preparation of oligochitosan-gelatin-magnesia feldspar nanofiber biological dressing.

Chitooligosaccharide 2g, gelatin 12g, feldspar 10g, 2,2,2-trifluoroethanol to 100ml.

Steps:

(1) Weigh 2g of chitosan oligosaccharide (molecular weight is 1000-2500Da, degree of polymerization is 2-8, obtained by physical degradation of chitosan) and 12g of gelatin, add to 2,2,2-trifluoro Swell overnight in ethanol and stir at 50°C for 1 h to obtain a transparent, viscous gel-like mixture.

(2) Use a ball mill to grind 10 g of feldspar feldspar, dry grind 5 times, grind for 6 minutes each time, and rotate at 1200 rpm until the particle size of feldspar feldspar powder is less than 10 μm, collect the dry powder, add it to the above-mentioned gelatinous mixture, and dry it at 50 Stir at ℃ for 1 h to obtain a uniform gel suspension;

(3) After the above-mentioned gel suspension is extruded at a constant speed by a syringe pump, it is forced to be sprayed and stretched to a collection device (fiber diameter is 1000nm) by a high-voltage electric field force, and flattened to form a fiber mat to obtain chitosan oligosaccharides - Gelatin-magnesia feldspar nanofiber biological dressing, wherein: the inner diameter of the spinneret used for electrospinning is 0.6mm, the injection flow rate is 0.6mL/h, the electric field strength is 12kV/cm, and the electrospinning distance is 15cm.

Example 4: Hygroscopic performance test of composite nanofiber biological dressing.

The biological dressing obtained in Examples 1-3 was cut into small pieces of 10mm×10mm, dried to a constant weight, placed in an environment with a relative humidity of 60%, and weighed regularly until moisture absorption equilibrium was reached. The moisture absorption rate (M) is calculated according to the following formula, where M _x is the weight after moisture absorption, and M ₀ is the dry weight before moisture absorption.

$\mathrm{<span\; class="notranslate">\; m</span>}<span\; class="notranslate">\; =</span>\frac{{\mathrm{<span\; class="notranslate">\; m</span>}}_{\mathrm{<span\; class="notranslate">\; x</span>}}<span\; class="notranslate">\; -</span>{\mathrm{<span\; class="notranslate">\; m</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}}}{{\mathrm{<span\; class="notranslate">\; m</span>}}_{\mathrm{<span\; class="notranslate">\; 0</span>}}}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 100</span>}\mathrm{<span\; class="notranslate">\; \%</span>}$

The results of the above-mentioned moisture absorption test experiment (expressed as "mean ± standard deviation") are shown in Table 1.

Table 1. Results of moisture absorption rate of composite nanofiber bio-dressings

Weighing time/h M/Example 1 M/Example 2 M/Example 3 4 1.5±0.43 1.8±0.32 1.9±0.24 8 3.6±0.66 4.2±0.45 5.7±0.38 12 7.4±0.25 8.8±0.43 9.2±0.22 twenty four 10.2±0.35 12.8±0.16 14.6±0.37 48 11.4±0.25 14.2±0.31 16.1±0.28 72 11.7±0.17 14.7±0.36 16.3±0.31

As can be seen from the results in Table 1, the biological dressing of the present invention has good hygroscopicity, and the different contents of chitosan and gelatin also affect the moisture absorption rate of the biological dressing, so it can ensure that the wound is in a humid environment, which is beneficial to Accelerate the formation of blood vessels and promote the formation of granulation tissue; at the same time, the humid environment maintains a constant temperature on the wound surface, which is conducive to the healing of tissues without scabs, avoids the new granulation tissue from being subjected to mechanical damage again, and reduces pain.

Claims (9)

1. oligochitosan-gelatin-akermanite nanofiber biological dressing, its by comprise oligochitosan, gelatin, akermanite and The gel suspension of solvent is prepared from by the method for electrostatic spinning.

Oligochitosan-gelatin the most according to claim 1-akermanite nanofiber biological dressing, it is characterised in that described In gel suspension, the mass concentration of oligochitosan is 0.1～5g/100mL, and the mass concentration of gelatin is 2～15g/100mL, and magnesium is yellow The mass concentration of Anhydrite is 1～20g/100mL.

Oligochitosan-gelatin the most according to claim 2-akermanite nanofiber biological dressing, it is characterised in that described In gel suspension, the mass concentration of oligochitosan is 0.3～3g/100mL, and the mass concentration of gelatin is 4～14g/100mL, and magnesium is yellow The mass concentration of Anhydrite is 1.5～10g/100mL.

Oligochitosan-gelatin the most according to any one of claim 1 to 3-akermanite nanofiber biological dressing, it is special Levying and be, described oligochitosan is the catabolite of chitosan, and molecular weight is 1000～2500Da, and the degree of polymerization is 2～8.

Oligochitosan-gelatin the most according to claim 4-akermanite nanofiber biological dressing, it is characterised in that described Degraded is completed by Physical, chemical method or enzyme process.

Oligochitosan-gelatin the most according to claim 1-akermanite nanofiber biological dressing, it is characterised in that described The mixture of the solvent any one or its arbitrary proportion in water, glacial acetic acid, formic acid, the 2,2,2 tfifluoroethyl alcohol.

7. oligochitosan-gelatin according to any one of claim 1 to 6-akermanite nanofiber biological dressing Preparation method, it comprises the following steps:

1) weigh oligochitosan and gelatin, join in solvent swelling overnight, in 45～60 DEG C stir 0.5～2 hour, obtain gel Shape mixture；

2) grind akermanite, until particle diameter is less than 10 μm, collects akermanite dry powder, join step 1) in the gel that obtains In shape mixture, stir 1～3 hour in 45～60 DEG C, obtain gel suspension；

3) by the method for electrostatic spinning, by step 2) in the gel suspension that obtains make a diameter of 100～1000nm receive Rice fiber, tiling forms fiber felt, obtains oligochitosan-gelatin-akermanite nanofiber biological dressing.

Preparation method the most according to claim 7, it is characterised in that step 2) described in grind use ball mill complete Becoming, dry grinding 4～6 times, each 2～8 minutes, rotating speed was 700～1200rpm.

Preparation method the most according to claim 7, it is characterised in that step 3) described in the parameter of electrostatic spinning as follows: Spinning head internal diameter is 0.6mm, injection flow be 0.2～1.5mL/h, electric field intensity is 5～20kV/cm, electrospinning spacing be 10～ 20cm。