CN106198359B - A kind of Arneth's count instrument optical system using integrating sphere - Google Patents
A kind of Arneth's count instrument optical system using integrating sphere Download PDFInfo
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- CN106198359B CN106198359B CN201610521608.8A CN201610521608A CN106198359B CN 106198359 B CN106198359 B CN 106198359B CN 201610521608 A CN201610521608 A CN 201610521608A CN 106198359 B CN106198359 B CN 106198359B
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- 230000003287 optical effect Effects 0.000 title claims abstract description 38
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- 230000005540 biological transmission Effects 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- 238000004364 calculation method Methods 0.000 claims abstract description 4
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- 239000007788 liquid Substances 0.000 claims description 6
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- 239000012530 fluid Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 210000003463 organelle Anatomy 0.000 description 2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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Abstract
The invention discloses a kind of Arneth's count instrument optical systems using integrating sphere, including lighting unit, sample unit, signal collection unit and the signal processing unit set gradually；Lighting unit, for making light beam transversal converge to sample unit, and the bar shaped diaphragm for converging to light beam longitudinally in signal collection unit；Sample unit, for passing sequentially through the cell in sample, and lighting unit issue light beam under generate multiple angles scattering light；Signal collection unit includes the integrating sphere for acquiring the scattering light of specified angle and being divided, and the detector for acquiring the scattering light after being divided；Signal processing unit for the scattering light data of pick-up probe transmission, and carries out corresponding calculation process.The present invention is divided using integrating sphere, can effectively avoid plane of crystal refraction and caused by polarization effect make the loss of signal, to improve test accuracy.And detector is connected directly with integrating sphere, can reduce the size of entire optical system.
Description
Technical field
The present invention relates to low cytometric analysis field more particularly to a kind of leukocyte differential count meters using integrating sphere
Number instrument optical system.
Background technique
Flow Cytometry is one kind in liquid fluid system, quickly measures the biological property of individual cells or organelle,
And specific cell or organelle are subject to from group the technology of categorised collection.Its main feature is that passing through quickly measurement Kurt electricity
Resistance, fluorescence, light scattering and light absorption quantitative determine cell DNA content, cell volume, protein content, enzymatic activity, cell
Many important parameters such as membrane receptor and surface antigen.Cell of different nature is separated according to these parameters, to obtain for biology
Learn the pure cell colony with medical research.
After haemocyte is dissolved or dyed by corresponding reagent, using sheath Flow Technique, make haemocyte successively one from sheath flow pool
A queuing for connecing one passes through.When cell passes through, using the hot spot irradiating cell of an ellipse, hot spot is upward in cell stream
Width should be slightly bigger than cell dia, to guarantee adequate illumination cell, should be slightly bigger than in the upward length of vertical cell stream
Sheath stream width.They can pass through laser irradiation area one by one.When a variety of different cells are irradiated with a laser, forward direction dissipates
Penetrate light, the signal of side scattered light and lateral fluorescence is all different.Using this difference, selection most can obviously distinguish two kinds or
The two-way of several cells of person.
Cell can generate scattering light when irradiating by hot spot.The power of small angle scattering light (2 ~ 8 degree) mainly characterizes thin
The volume information of born of the same parents, the power of large-angle scattered light (18 ~ 24 degree) then mainly characterize the information of cell interior particle complexity.
Two-dimensional coordinate system is constituted by size angle luminous intensity, then each cell passed through will be characterized as a point in coordinate system.It is more
After a cell passes through detection zone, it is formed scatter plot.
Currently, referring to FIG. 1, the optical information of cell size angle be generally divided into actual measurement two paths of signals with
Independent measurement, and this two paths of signals is usually to apply Amici prism 100 separated.But according to fresnel formula, light is in electricity
Reflection can be different and different with incident angle from refraction on dielectric interface, i.e. material is different to the transmission of p light with s light, this
When having led to light by crystal in Amici prism 1, entrained optical signalling cannot be completely passed, can be to needs
The signal of measurement causes damages.
Therefore, the existing technology needs to be improved and developed.
Summary of the invention
In view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of leukocyte differential counts using integrating sphere
Calculating instrument optical system, it is intended to which solution in the prior art divides the two-way light after cell scatters using Amici prism
Light, entrained optical signalling cannot be completely passed, and the signal that can be measured needs causes damages, to reduce test essence
The defect of degree.
Technical scheme is as follows:
A kind of Arneth's count instrument optical system using integrating sphere, wherein including the illumination list set gradually
Member, sample unit, signal collection unit and signal processing unit；Wherein:
The lighting unit for making light beam transversal converge to sample unit, and makes light beam longitudinally converge to signal collection
Bar shaped diaphragm in unit；
The sample unit is generated for passing sequentially through the cell in sample, and under the light beam that lighting unit issues
The scattering light of multiple angles；
The signal collection unit includes the integrating sphere for acquiring the scattering light of specified angle and being divided, and is used for
The detector of scattering light after acquisition light splitting；
The signal processing unit for the scattering light data of pick-up probe transmission, and carries out corresponding calculation process.
The Arneth's count instrument optical system using integrating sphere, wherein the lighting unit includes:
Laser tube；
Diaphragm, Beam limiting for issuing laser tube to specified light beam aperture；
Shaping lens carry out shaping for that will limit the light beam after light beam aperture；
First cylindrical mirror, for making light beam transversal converge to sample unit；
Second cylindrical mirror, the bar shaped diaphragm for converging to light beam longitudinally in signal collection unit.
The Arneth's count instrument optical system using integrating sphere, wherein the face type of the shaping lens is bent
Line is that the face type cross-section curve of spherical surface or the aspherical or described shaping lens is spherical surface or aspherical.
The Arneth's count instrument optical system using integrating sphere, wherein the light beam that the laser tube issues
After diaphragm limits light beam aperture, elliptical spot, the long axis of the elliptical spot are formed on the cell in sample unit
For 80 ~ 280um, short axle is 9 ~ 16um.
The Arneth's count instrument optical system using integrating sphere, wherein the sample unit includes sheath stream
Pond, the sheath flow pool internal liquid passage cross-section are round or square.
The Arneth's count instrument optical system using integrating sphere, wherein be provided with just on the integrating sphere
The entrance aperture that scattering light incidence is received to sample unit, the small angle for going out 2-8 ° of angle in incident light of scattering light emission
Spend light hole, and the wide-angle light hole for projecting 18-22 ° of angle in incident light of scattering light after diffusing reflection.
The Arneth's count instrument optical system using integrating sphere, wherein in the low-angle light hole
The central axis of mandrel and the entrance aperture on the same line, the central axis of the wide-angle light hole and the low-angle light passing
The central axis in hole.
The Arneth's count instrument optical system using integrating sphere, wherein the hole of the low-angle light hole
2d*tan8 ° of diameter；Wherein, d is equal to the linear distance between sample unit and entrance aperture.
The Arneth's count instrument optical system using integrating sphere, wherein the detector includes low-angle
Detector and wide-angle detector, wherein the exit of the low-angle light hole is provided with the low-angle detector, it is described
The exit of wide-angle light hole is provided with the wide-angle detector.
The utility model has the advantages that the present invention replaces Amici prism to be divided using integrating sphere, it is possible to prevente effectively from plane of crystal is rolled over
Penetrate and caused by polarization effect make the loss of signal, to improve the accuracy of test.And detector is connected directly with integrating sphere,
It can be substantially reduced the size of entire optical system, reduce the use for collecting mirror, more save the cost.
Detailed description of the invention
Fig. 1 is in the prior art using the schematic diagram of the Arneth's count instrument optical system of Amici prism.
Fig. 2 a is the knot of the Arneth's count instrument optical system preferred embodiment of the present invention using integrating sphere
Structure block diagram.
Fig. 2 b is showing for the Arneth's count instrument optical system preferred embodiment of the present invention using integrating sphere
It is intended to.
Fig. 3 is that the light beam of laser tube of the present invention sending forms showing for elliptical spot on the cell in sample unit
It is intended to.
Fig. 4 is the schematic diagram that the light beam that laser tube of the present invention issues forms hot spot on the second cylindrical mirror.
Specific embodiment
The present invention provides a kind of Arneth's count instrument optical system using integrating sphere, to make mesh of the invention
, technical solution and effect it is clearer, clear, the present invention is described in more detail below.It should be appreciated that described herein
Specific embodiment be only used to explain the present invention, be not intended to limit the present invention.
Please referring also to Fig. 2 a and Fig. 2 b, wherein Fig. 2 a is the Arneth's count instrument of the present invention using integrating sphere
With the structural block diagram of optical system preferred embodiment, Fig. 2 b is that the Arneth's count instrument of the present invention using integrating sphere is used
The schematic diagram of optical system preferred embodiment.The Arneth's count instrument optical system using integrating sphere, including according to
Lighting unit 200, sample unit 300, signal collection unit 400 and the signal processing unit 500 of secondary setting；Wherein:
The lighting unit 200 for making light beam transversal converge to sample unit, and makes light beam longitudinally converge to signal receipts
Collect the bar shaped diaphragm in unit；
The sample unit 300, for passing sequentially through the cell in sample, and the light beam issued in lighting unit 200
The lower scattering light for generating multiple angles；
The signal collection unit 400 includes the integrating sphere 8 for acquiring the scattering light of specified angle and being divided, and
For acquiring the detector (not marking in figure) of the scattering light after being divided；
The signal processing unit 500 for the scattering light data of pick-up probe transmission, and carries out at corresponding operation
Reason.
Integrating sphere 8 is also known as light and leads to ball, is a hollow complete spherical shell.Inner wall coating white diffusing reflection layer, and ball inner wall
Each point diffusion is uniform.The illuminance that light source generates on any point on ball wall is the illuminance superposition generated by multiple reflections light
Made of.Its basic working principle are as follows: after light is by input hole incidence, light is uniformly reflected and is diffused inside ball,
Uniform light distribution is formed on spherical surface, therefore the obtained light of delivery outlet is highly uniform diffusion light beam.And enter
Incident angle, spatial distribution and the polarity for penetrating light will not all impact the light intensity and the uniformity of output.Simultaneously as light
Line after being uniformly distributed inside integrating sphere by just projecting, and output intensity and input intensity ratio are about are as follows: and light output hole area/
Surface area inside integrating sphere.When can effectively reduce measurement using integrating sphere because on detector incident light source uneven distribution or
Slight error caused by beam deviation.
In the embodiment of the present invention, it is divided using integrating sphere 8 instead of Amici prism, it is possible to prevente effectively from plane of crystal
Refraction and caused by polarization effect make the loss of signal, to improve the accuracy of test.
Preferably, as shown in Figure 2 b, the lighting unit 200 includes:
Laser tube 1；
Diaphragm 2, Beam limiting for issuing laser tube 1 to specified light beam aperture；
Shaping lens 3 carry out shaping for that will limit the light beam after light beam aperture；
First cylindrical mirror 4, for making light beam transversal converge to sample unit；
Second cylindrical mirror 5, the bar shaped diaphragm for converging to light beam longitudinally in signal collection unit.
In the specific implementation, the light source of the laser tube 1 is 10w, and wavelength 635nm, wavelength is certain, and the angle of divergence can phase
It is same or different.The face type curve of the shaping lens 3 is transversal for the face type of spherical surface or the aspherical or described shaping lens 3
Surface curve is spherical surface or aspherical, the light beam for issuing to laser 1 collimated, uniformly, converge Shape correction.It is described to swash
The light beam that light pipe 1 issues is after diaphragm 2 limits light beam aperture and passes through the first cylindrical mirror 4, on the cell in sample unit 300
Elliptical spot is formed, referring to FIG. 3, the long axis of the elliptical spot is 80 ~ 280um, short axle is 9 ~ 16um；It is optimal,
The long axis of the elliptical spot is 110um, short axle 7um.The laser tube 1 forms ellipse on the cell in sample unit
In shape hot spot, Laser beam energy distribution is uniform, at elliptical spot center with it is poor away from optical energy density at the 5um of elliptical spot center
It is different to be no more than 20%.
Preferably, as shown in Figure 2 b, the sample unit 300 includes sheath flow pool 6, the 6 internal liquid access of sheath flow pool
Cross section is round or square, and when 6 internal liquid passage cross-section of sheath flow pool is round, its diameter is less than 25um, works as sheath stream
Its side length is less than 25um when 6 internal liquid passage cross-section of pond is square.The sample unit 300 is in addition to including sheath flow pool 6
It in addition, further include note sample needle and other pipelines.Wherein there was only sheath flow pool 6 in the optical path, the cell in sample can be one by one
By sheath flow pool 6, the light beam that lighting unit 200 issues can be radiated at the center of sheath flow pool 6, and focus at its center.In sheath
It flows in pond 6, sheath fluid is taken each cell wrapped up in sample and flowed successively through.
Further, bar shaped diaphragm 7 is additionally provided between the integrating sphere 8 and the sheath flow pool 6, the laser tube 1 is sent out
Light beam out also forms hot spot on bar shaped diaphragm 7 after diaphragm 2 and by the second cylindrical mirror 5, which is also ellipse,
This is also second focus of lighting unit 200 in the optical path, is focused light beam in X direction.
Preferably, it is provided with face sample unit 300 on the integrating sphere 8 to receive the entrance aperture of scattering light incidence, uses
In the low-angle light hole for going out 2-8 ° of angle in incident light of scattering light emission, and for making 18-22 ° of angle in incident light
The wide-angle light hole that is projected after diffusing reflection of scattering light.
Wherein, the central axis of the low-angle light hole and the central axis of the entrance aperture are on the same line, described big
The central axis of the central axis of angle light hole and the low-angle light hole.The aperture 2d* of the low-angle light hole
tan8°；Wherein, d is equal to the linear distance between sample unit 300 and entrance aperture.
The detector includes low-angle detector 10 and wide-angle detector 9, wherein the low-angle light hole goes out
It is provided with the low-angle detector 10 at mouthful, the exit of the wide-angle light hole is provided with the wide-angle detector 9.
After the low-angle light hole that 2-8 ° of scattering light emission goes out in the present invention, received by low-angle detector 10；18-22 ° of scattering light
It is reflected by 8 inner wall of integrating sphere, is received by wide-angle detector 9.Since detector and integrating sphere 8 are connected directly, Ke Yi great
The size of entire optical system is reduced greatly, reduces the use for collecting mirror, more save the cost.
In conclusion the Arneth's count instrument optical system of the present invention using integrating sphere, including successively set
Lighting unit, sample unit, signal collection unit and the signal processing unit set；Lighting unit, for converging light beam transversal
To sample unit, and the bar shaped diaphragm for converging to light beam longitudinally in signal collection unit；Sample unit, for making in sample
Cell passes sequentially through, and the scattering light of multiple angles is generated under the light beam that lighting unit issues；Signal collection unit includes using
In the integrating sphere for acquiring the scattering light of specified angle and being divided, and the detector for acquiring the scattering light after being divided；Letter
Number processing unit for the scattering light data of pick-up probe transmission, and carries out corresponding calculation process.The present invention uses integrating sphere
Be divided, can effectively avoid plane of crystal refraction and caused by polarization effect make the loss of signal, to improve test accuracy.
And detector is connected directly with integrating sphere, can reduce the size of entire optical system.
It should be understood that the application of the present invention is not limited to the above for those of ordinary skills can
With improvement or transformation based on the above description, all these modifications and variations all should belong to the guarantor of appended claims of the present invention
Protect range.
Claims (6)
1. a kind of Arneth's count instrument optical system using integrating sphere, which is characterized in that including the photograph set gradually
Bright unit, sample unit, signal collection unit and signal processing unit；Wherein:
The lighting unit for making light beam transversal converge to sample unit, and makes light beam longitudinally converge to signal collection unit
In bar shaped diaphragm；
The sample unit, for passing sequentially through the cell in sample, and lighting unit issue light beam under generate it is a variety of
The scattering light of angle；
The signal collection unit includes the integrating sphere for acquiring the scattering light of specified angle and being divided, and for acquiring
The detector of scattering light after light splitting；
The signal processing unit for the scattering light data of pick-up probe transmission, and carries out corresponding calculation process；
Face sample unit is provided on the integrating sphere to receive the entrance aperture of scattering light incidence, for making angle in incident light
The low-angle light hole that the scattering light emission that degree is 2-8 ° goes out, and for passing through 18-22 ° of angle in incident light of scattering light
The wide-angle light hole projected after diffusing reflection；
The detector includes low-angle detector and wide-angle detector, wherein the exit of the low-angle light hole is set
It is equipped with the low-angle detector, the exit of the wide-angle light hole is provided with the wide-angle detector；The small angle
Spend light hole central axis and the entrance aperture central axis on the same line, the central axis of the wide-angle light hole with
The central axis of the low-angle light hole.
2. using the Arneth's count instrument optical system of integrating sphere according to claim 1, which is characterized in that described
Lighting unit includes:
Laser tube；
Diaphragm, Beam limiting for issuing laser tube to specified light beam aperture；
Shaping lens carry out shaping for that will limit the light beam after light beam aperture；
First cylindrical mirror, for making light beam transversal converge to sample unit；
Second cylindrical mirror, the bar shaped diaphragm for converging to light beam longitudinally in signal collection unit.
3. using the Arneth's count instrument optical system of integrating sphere according to claim 2, which is characterized in that described
The face type curve of shaping lens is that the face type cross-section curve of spherical surface or the aspherical or described shaping lens is spherical surface or aspheric
Face.
4. using the Arneth's count instrument optical system of integrating sphere according to claim 2, which is characterized in that described
The light beam that laser tube issues forms elliptical spot after diaphragm limits light beam aperture on the cell in sample unit, described
The long axis of elliptical spot is 80 ~ 280um, and short axle is 9 ~ 16um.
5. using the Arneth's count instrument optical system of integrating sphere according to claim 1, which is characterized in that described
Sample unit includes sheath flow pool, and the sheath flow pool internal liquid passage cross-section is round or square.
6. using the Arneth's count instrument optical system of integrating sphere according to claim 1, which is characterized in that described
2d*tan8 ° of the aperture of low-angle light hole；Wherein, d is equal to the linear distance between sample unit and entrance aperture.
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| CN107219195B (en) * | 2017-05-23 | 2019-07-23 | 山东中医药大学附属医院 | A kind of blood leucocyte detection device and method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004294249A (en) * | 2003-03-27 | 2004-10-21 | Fuji Kiki Kogyo Kk | Photometric instrument |
| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
| CN101498646A (en) * | 2008-02-03 | 2009-08-05 | 深圳迈瑞生物医疗电子股份有限公司 | Forward-scattering signal inspection device and method, cell or particle analyzer |
| CN201311324Y (en) * | 2008-11-18 | 2009-09-16 | 中国计量科学研究院 | Integrating sphere light collector |
| CN102384889A (en) * | 2011-08-03 | 2012-03-21 | 长春迪瑞医疗科技股份有限公司 | Integrating spherical optical system of automatic fecal occult blood analyzer |
| CN102680379A (en) * | 2012-05-31 | 2012-09-19 | 长春迪瑞医疗科技股份有限公司 | Device for classifying and counting white cells by using even high-order aspherical laser shaping system |
| CN104215562A (en) * | 2013-05-31 | 2014-12-17 | 深圳迈瑞生物医疗电子股份有限公司 | Optical system of particle analyzer |
| CN104949910A (en) * | 2015-05-29 | 2015-09-30 | 广州埃克森生物科技有限公司 | Five-differential blood analyzer optical system |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1191325B1 (en) * | 2000-03-31 | 2007-12-12 | Japan as represented by President of Kobe University | Method and apparatus for detecting mastitis by using visible light and/or near infrared light |
| DE60218074T2 (en) * | 2001-03-29 | 2008-02-07 | Sysmex Corp. | flow cytometer |
| WO2005001436A1 (en) * | 2003-06-26 | 2005-01-06 | The Secretary Of State For Defence | Improvements to fluid borne particle analysers |
-
2016
- 2016-07-05 CN CN201610521608.8A patent/CN106198359B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004294249A (en) * | 2003-03-27 | 2004-10-21 | Fuji Kiki Kogyo Kk | Photometric instrument |
| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
| CN101498646A (en) * | 2008-02-03 | 2009-08-05 | 深圳迈瑞生物医疗电子股份有限公司 | Forward-scattering signal inspection device and method, cell or particle analyzer |
| CN201311324Y (en) * | 2008-11-18 | 2009-09-16 | 中国计量科学研究院 | Integrating sphere light collector |
| CN102384889A (en) * | 2011-08-03 | 2012-03-21 | 长春迪瑞医疗科技股份有限公司 | Integrating spherical optical system of automatic fecal occult blood analyzer |
| CN102680379A (en) * | 2012-05-31 | 2012-09-19 | 长春迪瑞医疗科技股份有限公司 | Device for classifying and counting white cells by using even high-order aspherical laser shaping system |
| CN104215562A (en) * | 2013-05-31 | 2014-12-17 | 深圳迈瑞生物医疗电子股份有限公司 | Optical system of particle analyzer |
| CN104949910A (en) * | 2015-05-29 | 2015-09-30 | 广州埃克森生物科技有限公司 | Five-differential blood analyzer optical system |
Non-Patent Citations (1)
| Title |
|---|
| 测量生物组织光学特性参数的单积分球技术;苏万钧等;《福建师范大学学报（自然科学版）》;20040315;第20卷(第1期);38-40页 |
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