CN1061817C - Disinfectant for silkworm - Google Patents

Abstract

The present invention provides a high efficiency broad-spectrum disinfectant prepared by compounding sodium dichloroisocyanurate and a right quantity of synergistic agents, wherein the sodium dichloroisocyanurate is a preparation with high chlorine and is used as a main agent, and the synergistic agents are used as an auxiliary agent and is added to the main agent. The high efficiency broad-spectrum disinfectant can be widely used for the disinfection of the cocoon houses and the cocoom tools of silk cocoon breeding, and overcomes the defects of unstable agricultural chemical effect, easy dissipation, narrow-spectrum sterilization, large corrosiveness, strong stimulation, high cost, etc. existing in the present generally used bleaching powder and aldehyde preparation disinfectants.

Description

Disinfactant for silkworm

The present invention relates to a kind of disinfactant for silkworm, be mainly used in silkworm room, the disinfection of tools for silkworm rearing of raw cocoon production and parent silkworm rearing, phase air sterillization and the mulberry leaf face sterilization that also can be used for breeding silkworms all has killing action to the polyhedrosis virus of silkworm, bacterial spore, fungus conidium, particulate protozoon spore etc.

At present in the sericulture process the bleaching powder disinfectant of normal use exist that drug effect shakiness, corrosivity are strong, the shortcoming of storage difficulties, and for example the agent of aldehyde preparation sterilizing also exists fungicidal spectrum not wide (can not play disinfective action simultaneously to mycosis, virus disease, bacteriosis and the pebrine of silkworm), and its strong impulse damages people's eyes and respiratory mucosa, influences health.It is big that above-mentioned two kinds of common disinfectantses also exist working concentration, causes the high shortcoming of disinfection cost.

The object of the present invention is to provide a kind of is host with high chlorinated product, and other adds the composite formula disinfactant for silkworm that synergist is an assistant agent, in order to sterilizations such as silkworm room, rearing instruments, to overcome the existing weak point of above-mentioned existing disinfectant.

The present invention mainly is made up of high chlorinated product, its ultimate constituent is: host sodium dichloro cyanurate (Sod-ium dichloroisocyanurate), assistant agent Efficacious Disinfeitant (Chlorinated lrisodiumphosphate), its weight proportion is: host 60--70 is than assistant agent 10--15.Its production technology is: in proportion main and auxiliary dose is loaded on respectively in the pouch, dress is done in a box or the bag in the lump then.During use, mix main and auxiliary two kinds of medicaments soluble in water, its liquor strength is generally 3500--4500ppm and is advisable, mode can adopt spraying, floods or splash, silkworm room, disinfection of tools for silkworm rearing usage amount are generally 225ml/ square metre, require to keep the moisture state more than half an hour just can make drug effect obtain best performance.

The pharmacology analysis and the mechanism of action: host sodium dichloro cyanurate of the present invention contains available chlorine and reaches 62--64.5%, and dry powder long term storage available chlorine is highly stable.Its assistant agent Efficacious Disinfeitant is inorganic chlorine-containing disinfectant, is the stable crystallization of a kind of chemical property, and its aqueous solution is alkalescence (pH value is 11.7 ± 0.2).Disinfection composition clorox in the host promotes it to discharge from sodium dichloro cyanurate because of being subjected to the alkalescence effect of assistant agent Efficacious Disinfeitant greatly, and improves fungicidal effectiveness.The disinfecting process of this disinfectant is: by the oxidation of hypochlorous acid to bacterial propagule, virus, fungal spore, bacterial spore, particulate protozoon spore etc., make its primary protein denaturation and lose pathogenecity, even the pathogene burn into can also be dissolved.

The present invention has broad-spectrum high efficacy, efficacy stability, is difficult for advantages such as lost, that corrosivity is weak, and disinfection cost is low, sees that according to several disinfectant consumption contrasts commonly used in the actual production silkworm room, a disinfection of tools for silkworm rearing agent usage amount are: 1 kilogram in bleaching powder, 2.1 yuan; 1.5 kilograms in formaldehyde, 3.3 yuan; Myristylpicolinum bromide 100 grams, 1.9 yuan; " Anaprox " 100 grams, 1.6 yuan.Can be clear that the present invention's's " Anaprox " superiority place from the data of following several groups of comparative trials.

One, experimental condition:

1, for the reagent thing: bleaching powder (main component content 26%)

Formaldehyde preparation (main component content 36%)

Myristylpicolinum bromide (main component content 80%)

Anaprox (product of the present invention)

2, for examination silkworm pathogenic microorganism: white stiff mattress, aspergillus conidium, soldier's gemma of falling the bacterium, NPB, CPB, particulate protozoon spore.

3, be that the spring is with planting Soviet Union 6 * Soviet Union 5 for examination silkworm kind

Two, test method:

(1), Anaprox of the present invention is to the fall ill mensuration of sterilization critical concentration of substance of silkworm main diseases

1, fungi and bacterium:

1 * 1 square centimeter of sterilization filter paper input is tested dipping in the bacterium liquid (50,000/cubic millimeter), take out drying, sterilization is 5 minutes in for reagent liquid, stops sterilization with neutralizer, cultivates in the input liquid nutrient medium, identifies Disinfection Effect.The results are shown in Table 1:

Table 1 pair fungi and bacterium Disinfection Effect

Chemicals treatment	White muscardine fungi	Aspergillus flavus	Soldier's gemma of falling the bacterium
				Do not sterilize	0/2×4	0/2×2	0/2×2
Bleaching powder 10000PPm	4/2×2	6/2×3	4/2×2
				Myristylpicolinum bromide 3500ppm	4/2×2	6/2×3	4/2×2
Anaprox 1000PPm	4/2×2	4/2×3	2/2×2
				Anaprox 1500PPm	4/2×2	8/2×4	4/2×2

Annotate: divide subrepresentation not have test mattress vitellarium number, denominator is represented duplicate block number and test number (TN).

2, viral polyhedron and particulate protozoon spore

NPB, CPB and particulate spore are used confession reagent liquid disinfectant 5 minutes respectively in test tube, stop sterilization with neutralizer, be mixed with 20000/ cubic millimeter of suitable polyhedron then, the suspension of the suitable 40000/ cubic millimeter of concentration of particulate spore, one age newly-hatched silkworm freshen food, raise 3 length of times, the virus disease investigation incidence of disease, the pebrine silkworm is dissected investigation and infects.The results are shown in Table 2:

Table 2 pair viral polyhedron and little malicious spore Disinfection Effect (%)

Chemicals treatment	NPB	CPB	The particulate spore
				Do not sterilize	100	89	100
Anaprox 10PPm	--	--	0
				Anaprox 500ppm	--	--	0
Anaprox 1000PPm	10.0	65.5	--
				Anaprox 1500PPm	0	6	--
Anaprox 2500ppm	0	0	--
				Blank	0	0	0

According to above test result,, be that thoroughly to kill critical concentration in 5 minutes be 1500PPm to example with the aspergillus flavus with reference to general chlorinated product active chlorine concentrations unit.Microbial activity to part representative pathogens body differentiates that thorough sterilization critical concentration was as follows in its 5 minutes:

Aspergillus flavus 1500PPm white muscardine fungi 1000PPm

Soldier's gemma of falling bacterium 1500PPm NPB 1500PPm

Particulate protozoon spore 10PPm CPB 2500PPm

(2), Anaprox is to the fall ill simulation sterilizing test of substance of silkworm main diseases

1, to mycosis simulation sterilizing prevent disease

Be applied to respectively on the preprepared aseptic slide in suspension (60,000/cubic millimeter) and aspergillus conidia (50,000/cubic millimeter) bacterium liquid with the mitogenetic spore of white muscardine fungi, drop into the reagent agent dipping respectively after 30 minutes after smoothening airing, drop into reagent removal in the culture dish that fills sterile water immediately, take out airing slightly then, each aqua sterilisa that adds mycillin with 3ml is washed dry doubling with the cause of disease on the slide and directly is inoculated in and plays silkworm silkworm body 2 ages, and subregion is supported and played the silkworm investigation incidence of disease to 4 ages.The results are shown in Table 3:

Table 3 Anaprox is to mycotic sterilizing prevent disease effect

Chemicals treatment	The white muscardine incidence of disease	The aspergillus flavus incidence of disease
			Do not sterilize	100	95
Bleaching powder (1%)	3	3
			Formaldehyde (2%)	0	8
Anaprox 1500PPm	2	3
			Anaprox 2000PPm	0	0
Blank	0	0

Anaprox 1500PPm, 2000PPm are remarkable to white muscardine and aspergillus disease control efficiency, have reached the sterilisation level of bleaching powder and formaldehyde typical concentrations fully.

2, to virus disease simulation sterilizing prevent disease

Each 1mlCPB, NPB suspension on aseptic slide respectively, smoothen airing, drop into reagent agent respectively, flood after 30 minutes, take out and drop into reagent removal in the culture dish that fills aqua sterilisa immediately, take out airing slightly then, each washes cause of disease with the 3ml aqua sterilisa, be coated onto the mulberry leaf back side and freshen food to playing silkworm 2 ages, subregion is raised to 4 ages and is played silkworm, investigates the incidence of disease.The results are shown in Table 4:

Table 4 Anaprox is to the sterilizing prevent disease effect (%) of virus disease

Chemicals treatment	The NPB incidence of disease	The CPB incidence of disease
			Do not sterilize	100	100
Bleaching powder (1%)	3.3	0
			Formaldehyde (2%)	1.7	23.3
Anaprox 1500PPm	3.3	23.3
			Anaprox 2000PPm	0	0
Blank	0	0

3, Anaprox is simulated sterilizing prevent disease to pebrine

With particulate spore (50,000/cubic millimeter) liquid, mixed with the Anaprox aqueous solution, after certain hour is disinfected, add in the 5% sodium thiosulfate liquid and the back is added sense of food to silkworm and dyed, dissect the infection conditions of microscopy tissue after normally raising two length of times.The results are shown in Table 5:

The protection effect (%) of table 5 pair pebrine

Chemicals treatment	Do not sterilize	Blank	Bleaching powder (1%)	Formaldehyde (2%)	Anaprox 1500PPm	Anaprox 2000PPm
							Infection rate	100	0	0	0	0	0

Observe under 600 power microscopes, through the spore outward appearance distortion that Anaprox is handled, refractivity descends, and infects the effect that has reached bleaching powder and formaldehyde fully according to the routine disinfection control.

(3), the silkworm toxic and side effect is differentiated:

1, bath disappears to the influence of silkworm egg

Bath disappears to silkworm egg, sterilizes investigation vitality after the reagent removal.The results are shown in Table 6:

The go on a hunger strike influence of vitality of table 6 pair egg-incubation and newly-hatched silkworm

Chemicals treatment	Bath disappears the time	Practical incubation rate (%)	Half death (my god)	All dead (my god)
					Bleaching powder (3 ‰)	10 (branches)	96.3	5	8
Formaldehyde (2%)	40 (branches)	97.0	5	7
					Anaprox 1500PPm	30 (branches)	97.0	5	8
Anaprox 2000PPm	30 (branches)	96.7	5	8

" Anaprox " has no adverse effects to the silkworm egg incubation rate and the vitality of going on a hunger strike.

2, the following influence of food to silkworm

" Anaprox " freshens food with 2000PPm, contrasts with bleaching powder (1%) and clear water: I, young silkworm are added food 2 times per age, and grown silkworm is added food 1 time every day; II, young silkworm are added food 1 time per age, and grown silkworm was added food in per two days 1 time.The results are shown in Table 7:

Table 7 freshens the influence of food to silkworm

Chemicals treatment	Play silkworm worm pupa rate (%) two ages	Silkworm (%) does not cocoon	Cocoon weight (%)
				Bleaching powder (I)	92.5	5.0	1.91
Bleaching powder (II)	95.0	0	1.95
				Clear water (II)	87.5	0	1.98
Sterilization is (I)	92.5	2.5	1.88
				Sterilization is (II)	95.5	0	1.93

(4), stability, the corrosivity test result of sterilization ichor: 1, stability:

The stability of table 8 Anaprox, bleaching powder disinfectant sterilization drug effect is measured:

Annotate: soldier's gemma of falling the bacterium 3.0 * 10 ⁴Individual/cubic millimeter; Aspergillus conidia 3.0 * 10 ⁴Individual/cubic millimeter.

25 ℃ of constant temperature of seasoning condition, dark is added a cover 25 ℃ of treatment temperatures.

The sterilization ichor that just has been made into as can be seen from Table 8 can keep the fungicidal effectiveness more much better than than bleaching fluid in 24 hours, it is similar to arrive 62 hours both fungicidal effectivenesses.From the variation of PH, in 24 hours also than bleaching fluid height.This can infer the killing power to the polyhedron germ, also is reasonable.

(2), corrosivity:

Observe in the test, not all corrosions easily of the metal device of blooming protection, as iron nail, bleaching fluid is the most serious, secondly is clear water, and Anaprox shows the lightest; If be coated with diaphragm as nickel, chromium, pitch-dark, show that then bleaching fluid is extremely serious corrosion, the sterilization ichor has the corrosion point slightly, the clear water situation is best.Total notion is that the corrosivity of sterilization ichor is significantly less than bleaching fluid.

Embodiment: sodium dichloro cyanurate 63 grams, Efficacious Disinfeitant 12 grams, attach together in one bag after the packing respectively, during use, two kinds of medicine systems are mixed house simultaneously be dissolved in 12.5 kg water, be dissolved into the sterilization soup, can sterilize a standard silkworm room, rearing instrument.

Claims (3)

1. a disinfactant for silkworm mainly is made up of high chlorinated product, it is characterized in that its ultimate constituent is: host sodium dichloro cyanurate, assistant agent Efficacious Disinfeitant; Its main and auxiliary dose of weight proportion is: host 60--70 is than assistant agent 10--15.

2. the production technology of disinfactant for silkworm according to claim 1 is characterized in that in proportion main and auxiliary dose being loaded on respectively in the pouch, is loaded in the lump then in a box or the bag.

3. the using method of disinfactant for silkworm according to claim 1, it is characterized in that main and auxiliary dose of mixing soluble in water, its liquor strength is generally 3500--4500ppm and is advisable, mode can adopt spraying, floods or splash, silkworm room, disinfection of tools for silkworm rearing usage amount are generally 225ml/ square metre, keep above moisture state half an hour.