CN106148184B - A kind of reagent cartridge being provided with magnetic bead transfer organization - Google Patents
A kind of reagent cartridge being provided with magnetic bead transfer organization Download PDFInfo
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- CN106148184B CN106148184B CN201510165627.7A CN201510165627A CN106148184B CN 106148184 B CN106148184 B CN 106148184B CN 201510165627 A CN201510165627 A CN 201510165627A CN 106148184 B CN106148184 B CN 106148184B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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Abstract
A kind of reagent cartridge being provided with magnetic bead transfer organization includes the first cavity being disposed adjacent and the second cavity and by first cavity and the second cavity interval region spaced apart；An accommodating chamber is formed in the interval region, be provided with the magnetic bead transfer organization magnetic bead in the first cavity being transferred in the second cavity in the accommodating chamber, the magnetic bead transfer organization sealing is embedded in the accommodating chamber, the magnetic bead transfer organization includes cylindricality rotational structure and pushes the lead screw of the cylindricality rotational structure rotation, and the lead screw pushes cylindricality rotational structure to rotate the transfer for realizing magnetic bead in accommodating chamber.The solution of the present invention, by between adjacent cavity be arranged magnetic bead transfer organization, make magnetic bead and be adsorbed in magnetic bead material to be extracted fully, efficiently shifted；In addition, not only self structure is simple for the reagent cartridge of the present invention, and it is easy to operate, the complexity of the matched equipment used can be effectively reduced.
Description
Technical field
The present invention relates to the preparation of nucleic acid or the protein consumptive materials more particularly to one kind used with detection to be provided with magnetic bead turn
Move the reagent cartridge of structure.
Background technology
With the transformation of medical model and the continuous development of personalized medicine, there is an urgent need to quick, accurate for medical test circle
Detection means, Molecular Detection then plays unique advantage.The basis of Molecular Detection be analyze detected person histocyte,
Hair, anticoagulation or dry bloodstain and formaldehyde fixes, gene and its expression product in the tissue of paraffin embedding, by from point
Nucleic acid (DNA and RNA) is completed in sub- level to detect, in disease once occurring even symptom, sign and biochemical change not yet occur
Before, detection can accurately just be made.
Currently, molecular detection technology mainly has making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology
Deng.Molecular Detection product is mainly used in the detection and physical examination of the clinical departments such as tumour, infection, heredity, Prenatal Screening
The heart, technical service center, third party testing agency and microbial rapid detection market etc..Currently, blood routine, cell
The measuring means such as, pathology and immunology develop towards automation, integration, standardization direction, but due to Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem.For example, the centrifugal column method or paramagnetic particle method that are used in biotechnology carry out nucleic acid extraction, generally requires and split
Four steps such as solution, combination, rinsing, elution, in addition subsequent making nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biological core
The detecting steps such as piece keep the full-automatic instrument of entire " from sample to result " very difficult, single with regard to effective in each step
For the transfer of ingredient, the prior art mainly adopts the modes shifted manually, i.e., by artificial mode by raw material or centre
Product is shifted in the reaction vessel of step successively in step successively and realizing, it is therefore apparent that the hand used in the prior art
Animal material branch mode, it is not only cumbersome, time-consuming and laborious.What is more also resides in whole operation process and be easy to cause pollution,
The purity of extract is influenced, and material is difficult fully, is efficiently shifted, and influences experimental result.Therefore, in view of Molecular Detection
The full-automatic instrument platform of its own technical complexity, " from sample to result " is few or there are many insoluble skills
Art problem, to realize material between each step it is abundant, efficiently transfer just needs the structure and work(in consumptive material and experimental facilities
Can go up carry out it is various consider, such as of high cost, poor sensitivity existing in the prior art, complicated, behaviour can be avoided
Make the problems such as excessively complicated with associated assay devices.
Invention content
For deficiency in the prior art, present invention solves the technical problem that being that providing one kind being provided with magnetic bead transfer knot
The reagent cartridge of structure makes magnetic bead and is adsorbed in the to be extracted of magnetic bead by the way that magnetic bead transfer organization is arranged between adjacent cavity
Material (protein, nucleic acid) is abundant, is efficiently shifted.
A kind of reagent cartridge being provided with magnetic bead transfer organization is provided present invention solves the technical problem that also residing in, not only certainly
Body structure is simple, and easy to operate, can be effectively reduced the complexity of the matched equipment used.
In order to solve the above-mentioned technical problem, the reagent cartridge packet provided in an embodiment of the present invention for being provided with magnetic bead transfer organization
The interval region for including the first cavity being disposed adjacent and the second cavity and being spaced apart first cavity and the second cavity；It should
An accommodating chamber is formed in interval region, being provided with one in the accommodating chamber is transferred to the magnetic bead in the first cavity in the second cavity
Magnetic bead transfer organization, magnetic bead transfer organization sealing is embedded in the accommodating chamber；
The magnetic bead transfer organization includes cylindricality rotational structure and pushes the lead screw of the cylindricality rotational structure rotation, described
Cylindricality rotational structure is set in the accommodating chamber and forms sealed spacer region by the first cavity and the second cavity with accommodating chamber
It separates, the cylindricality rotational structure is provided with a threaded hole along its central axes, and internal thread is provided in the threaded hole, the interior spiral shell
Line and the external screw thread of lead screw outer surface match；
The side of the cylindricality rotational structure is provided with the adsorbing plane for accommodating magnetic bead, is additionally provided in the interval region
Adsorb the magnet of magnetic bead；The adsorbing plane shifts the previous dynasty to first cavity in magnetic bead, and magnetic bead is adsorbed to by the magnet
In the adsorbing plane；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter the spiral shell of cylindricality rotational structure along cylindricality rotational structure
In pit, the internal thread that the external screw thread of lead screw acts on threaded hole pushes cylindricality rotational structure to turn in accommodating chamber around its axis
Dynamic, the magnetic bead of the adsorbing plane and adsorbing plane attachment is turned to towards in the region of the second cavity.
A kind of reagent being provided with magnetic bead transfer organization provided as the preferred embodiment of the present invention, the embodiment of the present invention
Cartridge further comprises some or all of following technical characteristic：
Preferably, the lead screw is provided with a through-hole along wherein axis direction, the magnet intert when magnetic bead shifts into
Magnetic bead is adsorbed in the through-hole, the magnet exits the through-hole after completing magnetic bead transfer.
Preferably, there are two the magnetic for being parallel to the cylindricality rotational structure axis for the both sides setting of the magnetic bead transfer organization
Body, the magnet generate the opposite toroidal magnetic field in direction.
Preferably, the magnet is permanent magnet or electromagnet.
Preferably, the joining place of first cavity and interval region is set as the contraction structure become narrow gradually, first
The cohesive position of cavity and cylindricality rotational structure, the first cavity are contracted to width corresponding with the adsorbing plane.
Preferably, the joining place of second cavity and interval region is set as the contraction structure become narrow gradually, second
The cohesive position of cavity and cylindricality rotational structure, the second cavity are contracted to width corresponding with the adsorbing plane.
Preferably, first cavity and the second cavity are arranged in parallel, in magnetic bead transfer process, the cylindricality rotational structure
Half cycle is rotated, the lead screw is external screw thread or externally threaded half pitch along the axial actuating length of cylindricality rotational structure.
Preferably, the magnetic bead transfer organization further includes a gland, and the gland is set to the lead screw periphery and suppresses
It is fixed in being offered on the receiving cavity wall that the cylindricality rotational structure and the first cavity and the second cavity are mutually linked, the gland
The guide through hole of the position lead screw, the lead screw extend to the cylindricality rotational structure through the guide through hole.
Preferably, the externally threaded end set of the lead screw has chamfering.
In addition, the reagent cartridge provided in an embodiment of the present invention for being provided with magnetic bead transfer organization, include in the reagent cartridge
The reagent cartridge described in multiple said programs being mutually linked.It is i.e. provided in an embodiment of the present invention to be provided with magnetic bead transfer organization
Reagent cartridge include first, second and third ... ..n cavitys are provided with magnetic bead transfer organization between adjacent two cavitys.
The reagent cartridge provided in an embodiment of the present invention for being provided with magnetic bead transfer organization, has the advantages that：
The reagent cartridge provided in an embodiment of the present invention for being provided with magnetic bead transfer organization by the cavity being disposed adjacent it
Between magnetic bead transfer organization, the side of the cylindricality rotational structure in the magnetic bead transfer organization is provided with the adsorbing plane for accommodating magnetic bead,
Adsorbing plane shifts the previous dynasty to first cavity in magnetic bead, and magnetic bead is adsorbed in the adsorbing plane by magnet；Magnetic bead shifts
When, lead screw pushes cylindricality rotational structure to be rotated in accommodating chamber, and the magnetic bead that adsorbing plane adheres to is made to turn in the second cavity, from
And realize magnetic bead and be adsorbed in magnetic bead material to be extracted (protein, nucleic acid) fully, efficiently shifted.To overcome core
In the extractions of the substances such as acid, albumen, detection process between each step due to the linkings operation such as material transfer existing for trivial operations,
The problem of sample or reagent vulnerable to pollution.In addition, the reagent cartridge for being provided with magnetic bead transfer organization of the present invention, not only itself is tied
Structure is simple, and easy to operate, due to the ingehious design of cylindricality rotational structure and lead screw in magnetic bead transfer organization, as long as along axis
To promotion lead screw, you can by the propelled longitudinally rotation for being converted to cylindricality rotational structure of lead screw, make and the matched column of wire rod thread
Shape rotational structure rotates in accommodating chamber, and the magnetic bead that adsorbing plane adheres to is turned in the second cavity.Therefore, in the auxiliary of periphery
The rotating machinery for not needing to be arranged and being transported exclusively for magnetic bead and rotational power being provided is helped in equipment, it is only necessary to provide axial motive force
Power source, to significantly reduce the complexity of the matched equipment used.
Description of the drawings
Fig. 1 is the structural schematic diagram for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
One.
Fig. 2 is the structural schematic diagram for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Two.
Fig. 3 is the structure for the lead screw being provided in the reagent cartridge of magnetic bead transfer organization that the preferred embodiment of the present invention provides
Schematic diagram.
Fig. 4 is the cylindricality rotation knot being provided in the reagent cartridge of magnetic bead transfer organization that the preferred embodiment of the present invention provides
The structural schematic diagram of structure.
Fig. 5 is the cavity body structure being provided in the reagent cartridge of magnetic bead transfer organization that the preferred embodiment of the present invention provides
Structural schematic diagram.
Specific implementation mode
The embodiment of the present invention provides a kind of reagent cartridge being provided with magnetic bead transfer organization：The first chamber being disposed adjacent
Body and the second cavity and the interval region for being spaced apart first cavity and the second cavity；One is formed in the interval region to hold
It receives chamber, a magnetic bead transfer organization being transferred to the magnetic bead in the first cavity in the second cavity, institute is provided in the accommodating chamber
The sealing of magnetic bead transfer organization is stated to be embedded in the accommodating chamber；The magnetic bead transfer organization includes cylindricality rotational structure and promotion institute
The lead screw of cylindricality rotational structure rotation is stated, the cylindricality rotational structure is set in the accommodating chamber and is formed with accommodating chamber and sealed
Interval region separates the first cavity and the second cavity, and the cylindricality rotational structure is provided with a threaded hole, institute along its central axes
It states and is provided with internal thread in threaded hole, the internal thread and the external screw thread of lead screw outer surface match；The cylindricality rotational structure
Side is provided with the adsorbing plane for accommodating magnetic bead, and the magnet of absorption magnetic bead is additionally provided in the interval region；The absorption is flat
The previous dynasty is shifted to first cavity in face in magnetic bead, and magnetic bead is adsorbed in the adsorbing plane by the magnet；When magnetic bead shifts,
Lead screw, lead screw is axially pushed to enter in the threaded hole of cylindricality rotational structure along cylindricality rotational structure, the external screw thread of lead screw acts on
The internal thread of threaded hole pushes cylindricality rotational structure to be rotated in accommodating chamber around its axis, and the adsorbing plane and adsorbing plane are attached
The magnetic bead is turned to towards in the region of the second cavity.
Wherein, the lead screw is provided with a through-hole along wherein axis direction, and the magnet interts when magnetic bead shifts into institute
It states and adsorbs magnetic bead in through-hole, the magnet exits the through-hole after completing magnetic bead transfer.
Wherein, there are two the magnetic for being parallel to the cylindricality rotational structure axis for the both sides setting of the magnetic bead transfer organization
Body, the magnet generate the opposite toroidal magnetic field in direction.
Wherein, the magnet is permanent magnet or electromagnet.
Wherein, the joining place of first cavity and interval region is set as the contraction structure become narrow gradually, in the first chamber
The cohesive position of body and cylindricality rotational structure, the first cavity are contracted to width corresponding with the adsorbing plane.
Wherein, the joining place of second cavity and interval region is set as the contraction structure become narrow gradually, in the second chamber
The cohesive position of body and cylindricality rotational structure, the second cavity are contracted to width corresponding with the adsorbing plane.
Wherein, first cavity and the second cavity are arranged in parallel, and in magnetic bead transfer process, the cylindricality rotational structure turns
Dynamic half cycle, the lead screw are external screw thread or externally threaded half pitch along the axial actuating length of cylindricality rotational structure.
Wherein, the magnetic bead transfer organization further includes a gland, the gland be set to the lead screw periphery and suppress in
The receiving cavity wall that the cylindricality rotational structure and the first cavity and the second cavity are mutually linked offers positioning on the gland
The guide through hole of the lead screw, the lead screw extend to the cylindricality rotational structure through the guide through hole.
Wherein, the externally threaded end set of the lead screw has chamfering.
Wherein, the reagent cartridge provided in an embodiment of the present invention for being provided with magnetic bead transfer organization includes in the reagent cartridge
The reagent cartridge described in multiple said programs being mutually linked.It is i.e. provided in an embodiment of the present invention to be provided with magnetic bead transfer organization
Reagent cartridge include first, second and third ... ..n cavitys are provided with magnetic bead transfer organization between adjacent two cavitys.
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without creative efforts
Embodiment shall fall within the protection scope of the present invention.
Fig. 1 and 2 is the structural representation for the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides
Figure.The reagent cartridge for being provided with magnetic bead transfer organization is applied in nucleic acid or albumen preparation and detection device, it is intended to be nucleic acid
Or albumen prepare and detection process in material transfer between adjacent step provide it is a kind of efficiently, efficiently solution, but simultaneously
It is not limited, in practical applications, the extraction box can also be applied to the extraction of other biological extract.
As shown in Figs. 1-5, the reagent cartridge for being provided with magnetic bead transfer organization that the preferred embodiment of the present invention provides includes the
One cavity 10 and the second cavity 20 and the interval region 30 for being spaced apart the first cavity 10 and the second cavity 20.From Fig. 1 and 2
Structure show that the first cavity 10 and the second cavity 20 are open-type structure, in fact, this be only the present invention a kind of reality
Existing mode, in other embodiments of the invention, the first cavity 10 and the second cavity 20 can also be arranged as required in addition to
Except septal area domain 30, other each faces are closed cavity body structure, and when the examination for being provided with magnetic bead transfer organization of the present invention
When agent cartridge is used in extraction and the test experience of nucleic acid, other than interval region 30, other each faces are closed cavity knot
Structure is more commonly used structure.
When specific implementation, an accommodating chamber 31 is formed in the interval region 30, one is provided with by the first cavity in accommodating chamber 31
Magnetic bead in 10 is transferred to the magnetic bead transfer organization 40 in the second cavity, and the sealing of magnetic bead transfer organization 40 is embedded in accommodating chamber
31.Wherein, magnetic bead transfer organization 40 includes cylindricality rotational structure 41 and pushes the lead screw 42 of 41 rotation of cylindricality rotational structure.Specifically
During realization, cylindricality rotational structure 41 is set in accommodating chamber and forms sealed spacer region by the first cavity 10 with accommodating chamber
20 are separated with the second cavity, cylindricality rotational structure 41 is provided with a threaded hole 411 along its central axes, is provided in threaded hole 411
Internal thread 412, the internal thread 412 and the external screw thread 421 of 42 outer surface of lead screw match.When lead screw is along cylindricality rotational structure 41
When being longitudinally propelling, by screw-driven, 42 axial translation of lead screw is converted into the rotation of cylindricality rotational structure 41, cylindricality rotational structure
41 rotate in accommodating chamber.
For the ease of screwing, the externally threaded end set of lead screw has chamfering 4211.In addition, magnetic bead transfer organization 40 also wraps
Include a gland 43.As shown in Fig. 2, gland 43 is set to the lead screw periphery and suppresses in the cylindricality rotational structure and the first chamber
The receiving cavity wall that body 10 and the second cavity 20 are mutually linked offers the guide through hole 431 for positioning the lead screw on gland 43,
Lead screw 42 extends to cylindricality rotational structure 41 through guide through hole 431, to play to 42 good fixation of lead screw and be oriented to work
With.
In order to fully, efficiently shift magnetic bead, the side of cylindricality rotational structure 41 is provided with the absorption for accommodating magnetic bead
Plane 413 is additionally provided with the magnet (not shown) of absorption magnetic bead in interval region 30.It is, in principle, that the present invention is mainly
By being used as extraction nucleic acid, albumen or other macromolecular bio-extracts, magnetic bead using the magnetic-particle including magnetic bead
The suction-operated to nucleic acids in samples, albumen or other macromolecular bio-extracts, the first cavity 10 are realized in the first cavity 10
Nucleic acid to be extracted, albumen or other macromolecular bio-extracts are adsorbed on magnetic bead in middle mixed material.At this point, i.e. magnetic bead
Before transfer, magnetic bead is adsorbed in adsorbing plane 413 by adsorbing plane 413 towards the first cavity 10, magnet；When magnetic bead shifts,
Along the axial promotion lead screw 42 of cylindricality rotational structure 41, lead screw 42 enters in the threaded hole 411 of cylindricality rotational structure, outside lead screw 42
The internal thread 412 that screw thread 421 acts on threaded hole pushes cylindricality rotational structure to be rotated in accommodating chamber around its axis, adsorbing plane
413 and adsorbing plane 413 in the magnetic bead that accommodates turn in the region towards the second cavity 10.By by cylindricality rotational structure 41
Side local setting be adsorbing plane 413, and make adsorbing plane 413 absorption the previous dynasty to the first cavity 10, waited for that magnetic bead existed
It is adsorbed onto under the action of magnetic force on the adsorbing plane 413, then pushes cylindricality rotational structure 41 to rotate through receiving by lead screw 42
Chamber can form arcuate gap in rotation process between adsorbing plane 413 and the curved surface cavity wall of accommodating chamber, and be adsorbed on suction
Magnetic bead in attached plane 413 has because the presence of arcuate gap thus falls off without being received the cavity wall of chamber and scraping off to realize
Effect ground material transfer, while except other positions of adsorbing plane 413 can be with the sealing of accommodating chamber 31 in cylindricality rotational structure 41
Match, ensure that mutual independence of the unclassified stores in addition to magnetic bead between the first cavity and the second cavity.
In addition, from the point of view of the structure setting of the first cavity 10 and the second cavity 20 with the joining place of interval region, in order to
Enough fully, magnetic bead is efficiently shifted, the joining place of the first cavity 10 and interval region 30 is set as the contraction structure become narrow gradually,
In the cohesive position of the first cavity 10 and cylindricality rotational structure, the first cavity 10 is contracted to corresponding with the adsorbing plane 413
Width.Correspondingly, the joining place of the second cavity 20 and interval region is also configured as the contraction structure become narrow gradually, second
The cohesive position of cavity 20 and cylindricality rotational structure 41, the second cavity 20 are contracted to width corresponding with the adsorbing plane 413.
For this purpose, the joining place of the first cavity 10 and the second cavity 20 and interval region may be configured as the structure that chamfering receives side, also may be used certainly
In a manner of as shown in Figure 5, set close to itself and interval region the width of the first cavity 10 and the second cavity 20 to
Structure gradually narrowed during joining place.
In a preferred embodiment of the invention, the first cavity 10 and the second cavity 20 are arranged in parallel, therefore, magnetic bead transfer
When, lead screw 42 needs that cylindricality rotational structure 41 is pushed to rotate half cycle, correspondingly, the axial direction of lead screw 42 in accommodating chamber
This size of translational thrust is half pitch.Certainly in other embodiments of the present invention, the first cavity 10 and the second cavity 20
Not parallel can be arranged and the two there are certain angles, and at this point, lead screw 42 promotes size and 41 angle of cylindricality rotational structure
It is determined also by the angle between the first cavity 10 and the second cavity 20, to keep the magnetic bead in the first cavity 10 accurate
Really, it is efficiently transferred in the second cavity.
As shown in figs. 1 and 3, in the preferred embodiment of the present invention 1, lead screw 42 is provided with a through-hole along wherein axis direction
422, magnet interts when magnetic bead shifts adsorbs magnetic bead in through-hole, and magnet exits through-hole 422 after completing magnetic bead transfer.This scheme
In, the working method of magnetic bead transfer organization 40 is：Before magnetic bead transfer, for adsorbing plane 413 towards the first cavity 10, magnet is interspersed
Enter in through-hole 422 and adsorb magnetic bead, magnetic bead is adsorbed in adsorbing plane 413；When magnetic bead shifts, along 41 axis of cylindricality rotational structure
To pushing lead screw 42, lead screw 42 to enter in the threaded hole 411 of cylindricality rotational structure, the external screw thread 421 of lead screw 42 acts on screw thread
The internal thread 412 in hole pushes cylindricality rotational structure to be rotated in accommodating chamber around its axis, adsorbing plane 413 and adsorbing plane 413
The magnetic bead of interior receiving is turned to towards in the region of the second cavity 10；When the magnetic accommodated in adsorbing plane 413 and adsorbing plane 413
Pearl turns to towards after the second cavity 10, and magnet exits in through-hole 422, suction-operated of the magnetic bead due to losing magnet magnetic force, point
It is dissipated in the second cavity 20.
In the embodiment of the present invention 2, there are two be parallel to cylindricality rotational structure axis for the both sides setting of magnetic bead transfer organization
The magnet of line, magnet generates the opposite toroidal magnetic field in direction, to make magnetic bead be moved to adsorbing plane 413.In this scheme, magnetic bead
The working method of transfer organization 40 is：Before magnetic bead transfer, adsorbing plane 413 is towards the first cavity 10, and magnetic bead is in toroidal magnetic field
It under the magneticaction of formation, concentrates in adsorbing plane 413, and this magnetic direction is identical as the shift direction of magnetic bead；In magnetic
When pearl shifts, along the axial promotion lead screw 42 of cylindricality rotational structure 41, lead screw 42 enters in the threaded hole 411 of cylindricality rotational structure,
The internal thread 412 that the external screw thread 421 of lead screw 42 acts on threaded hole pushes cylindricality rotational structure to turn in accommodating chamber around its axis
Dynamic, the magnetic bead accommodated in adsorbing plane 413 and adsorbing plane 413 is turned to towards in the region of the second cavity 10.
For magnet selection with from the point of view of material, when specific implementation, magnet is permanent magnet or electromagnet.The present invention's
Permanent magnet or can be extraordinary preferred embodiment by the electromagnet of power control is selected in embodiment 1.And in this hair
In bright embodiment two, required toroidal magnetic field, and the effect in magnetic field can not only easily and efficiently be generated using electromagnet
Time (i.e. magnetic bead transfer time) can be accurately controlled, and magnetic bead is from adsorbing plane after magnetic bead is transferred to the second cavity
It is further reacted in rapid dispersion to the second cavity.
It should be noted that from Fig. 1 and 2 structure can be seen that it is provided in an embodiment of the present invention be provided with magnetic bead turn
The reagent cartridge for moving structure only includes 20 two cavitys being disposed adjacent of the first cavity 10 and the second cavity.In fact, of the invention
In preferred embodiment using only there are two cavity reagent cartridge as the present invention program a kind of preferred embodiment, be in order to from
Simple structure is started with, and illustrates that material transfer of the present invention between adjacent step in nucleic acid or albumen preparation and detection process carries
One kind of confession efficiently, efficiently solution, but be not limited thereto, in practical applications, due to nucleic acid extraction and detection
Be related in the process multiple steps material transfer, have the material transfer step for being related to realizing by magnetic bead it is multiple,
It therefore, in other embodiments of the invention can also be comprising described in the multiple said programs being mutually linked in the reagent cartridge
Reagent cartridge.The reagent cartridge i.e. provided in an embodiment of the present invention for being provided with magnetic bead transfer organization include first and second,
Three ... ..n cavitys are provided with magnetic bead transfer organization between adjacent two cavitys.
The reagent cartridge for being provided with magnetic bead transfer organization of above-described embodiment announcement passes through between the cavity being disposed adjacent
Magnetic bead transfer organization, the side of the cylindricality rotational structure in the magnetic bead transfer organization are provided with the adsorbing plane for accommodating magnetic bead, inhale
Attached plane shifts the previous dynasty to first cavity in magnetic bead, and magnetic bead is adsorbed in the adsorbing plane by magnet；When magnetic bead shifts,
Lead screw pushes cylindricality rotational structure to be rotated in accommodating chamber, and the magnetic bead that adsorbing plane adheres to is made to turn in the second cavity, to
Realization magnetic bead and the material to be extracted (protein, nucleic acid) for being adsorbed in magnetic bead are abundant, are efficiently shifted.To overcome core
In the extractions of the substances such as acid, albumen, detection process between each step due to the linkings operation such as material transfer existing for trivial operations,
The problem of sample or reagent vulnerable to pollution.In addition, the reagent cartridge for being provided with magnetic bead transfer organization of the present invention, not only itself is tied
Structure is simple, and easy to operate, due to the ingehious design of cylindricality rotational structure and lead screw in magnetic bead transfer organization, as long as along axis
To promotion lead screw, you can by the propelled longitudinally rotation for being converted to cylindricality rotational structure of lead screw, make and the matched column of wire rod thread
Shape rotational structure rotates in accommodating chamber, and the magnetic bead that adsorbing plane adheres to is turned in the second cavity.Therefore, in the auxiliary of periphery
The rotating machinery for not needing to be arranged and being transported exclusively for magnetic bead and rotational power being provided is helped in equipment, it is only necessary to provide axial motive force
Power source, to significantly reduce the complexity of the matched equipment used.
The above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also considered as
Protection scope of the present invention.
Claims (10)
1. a kind of reagent cartridge being provided with magnetic bead transfer organization, which includes the first cavity and second being disposed adjacent
Cavity and the interval region for being spaced apart first cavity and the second cavity；It is characterized in that：It is formed in the interval region
One accommodating chamber is provided with the magnetic bead that one is transferred to the magnetic bead in the first cavity in the second cavity in the accommodating chamber and shifts knot
Structure, the magnetic bead transfer organization sealing are embedded in the accommodating chamber；
The magnetic bead transfer organization includes cylindricality rotational structure and pushes the lead screw of the cylindricality rotational structure rotation, the cylindricality
Rotational structure is set in the accommodating chamber and forms sealed spacer region with accommodating chamber and separates the first cavity and the second cavity,
The cylindricality rotational structure is provided with a threaded hole along its central axes, and internal thread is provided in the threaded hole, the internal thread with
The external screw thread of lead screw outer surface matches；
The side of the cylindricality rotational structure is provided with the adsorbing plane for accommodating magnetic bead, and absorption is additionally provided in the interval region
The magnet of magnetic bead；The adsorbing plane shifts the previous dynasty to first cavity in magnetic bead, and magnetic bead is adsorbed to described by the magnet
In adsorbing plane；When magnetic bead shifts, lead screw, lead screw is axially pushed to enter the threaded hole of cylindricality rotational structure along cylindricality rotational structure
In, the internal thread that the external screw thread of lead screw acts on threaded hole pushes cylindricality rotational structure to be rotated in accommodating chamber around its axis, institute
The magnetic bead for stating adsorbing plane and adsorbing plane attachment is turned to towards in the region of the second cavity.
2. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：The lead screw is along wherein
Axis direction is provided with a through-hole, and the magnet interts when magnetic bead shifts adsorbs magnetic bead in the through-hole, completes magnetic bead and turns
The magnet exits the through-hole after shifting.
3. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：The magnetic bead transfer knot
There are two the magnet for being parallel to the cylindricality rotational structure axis, the magnet generates the opposite annular magnetic in direction for the both sides setting of structure
.
4. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：The magnet is permanent magnetism
Body or electromagnet.
5. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：First cavity with
The joining place of interval region is set as the contraction structure become narrow gradually, in the cohesive position of the first cavity and cylindricality rotational structure,
First cavity is contracted to width corresponding with the adsorbing plane.
6. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：Second cavity with
The joining place of interval region is set as the contraction structure become narrow gradually, in the cohesive position of the second cavity and cylindricality rotational structure,
Second cavity is contracted to width corresponding with the adsorbing plane.
7. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：First cavity and
Second cavity is arranged in parallel, and in magnetic bead transfer process, the cylindricality rotational structure rotates half cycle, and the lead screw is rotated along cylindricality and tied
The axial actuating length of structure is external screw thread or externally threaded half pitch.
8. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：The magnetic bead transfer knot
Structure further includes a gland, the gland be set to the lead screw periphery and suppress in the cylindricality rotational structure and the first cavity and
The receiving cavity wall that second cavity is mutually linked offers the guide through hole for positioning the lead screw, the lead screw on the gland
The cylindricality rotational structure is extended to through the guide through hole.
9. the reagent cartridge as described in claim 1 for being provided with magnetic bead transfer organization, it is characterised in that：The outer spiral shell of the lead screw
The end set of line has chamfering.
10. a kind of reagent cartridge being provided with magnetic bead transfer organization, it is characterised in that：Include to be mutually linked in the reagent cartridge
Multiple reagent cartridges as described in any one of claim 1-9.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510165627.7A CN106148184B (en) | 2015-04-09 | 2015-04-09 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
| PCT/CN2015/087220 WO2016161739A1 (en) | 2015-04-09 | 2015-08-17 | Reagent cartridge provided with magnetic bead transferring structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510165627.7A CN106148184B (en) | 2015-04-09 | 2015-04-09 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
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| Publication Number | Publication Date |
|---|---|
| CN106148184A CN106148184A (en) | 2016-11-23 |
| CN106148184B true CN106148184B (en) | 2018-08-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510165627.7A Active CN106148184B (en) | 2015-04-09 | 2015-04-09 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN106148184B (en) |
| WO (1) | WO2016161739A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296330B (en) * | 2015-11-13 | 2018-10-02 | 奥然生物科技（上海）有限公司 | Liquid level inclined reagent cartridge when a kind of material transfer |
| US11433402B2 (en) | 2017-07-19 | 2022-09-06 | Amgen Inc. | Magnetic assisted separation apparatuses and related methods |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN88200247U (en) * | 1988-01-18 | 1988-11-09 | 赵书敏 | Quick cleaning equipment for magnet |
| GB0319671D0 (en) * | 2003-08-21 | 2003-09-24 | Secr Defence | Apparatus for processing a fluid sample |
| CN100484632C (en) * | 2004-11-10 | 2009-05-06 | 横河电机株式会社 | Chemical reaction box, its producing process and chemical reaction box driving system |
| CN101301632B (en) * | 2004-11-10 | 2010-12-29 | 横河电机株式会社 | Chemical reaction kit, production method thereof and chemical reaction kit driving system |
| CN201176432Y (en) * | 2007-12-21 | 2009-01-07 | 凯晟生物科技（上海）有限公司 | Full automatic instrument for extracting nucleic acid |
| JP5311518B2 (en) * | 2008-10-06 | 2013-10-09 | コーニンクレッカ フィリップス エヌ ヴェ | Microfluidic device |
| CN201313074Y (en) * | 2008-11-24 | 2009-09-23 | 江苏正昌粮机股份有限公司 | Permanent magnet drum |
| US8268264B2 (en) * | 2009-02-09 | 2012-09-18 | Caprotec Bioanalytics Gmbh | Devices, systems and methods for separating magnetic particles |
| CN101538612A (en) * | 2009-04-16 | 2009-09-23 | 上海科华生物工程股份有限公司 | Integrated blood nucleic acid screening platform |
| CN101798024A (en) * | 2009-12-28 | 2010-08-11 | 苏州工业园区泰格电子科技有限公司 | Magnetic force delivering device |
| CN101851617B (en) * | 2010-04-28 | 2012-11-21 | 长春市博坤生物科技有限公司 | Extracting method of viral nucleic acid capable of being used for high flux full automation extraction by magnetic bead method |
| CN101865924B (en) * | 2010-06-26 | 2013-07-03 | 上海交通大学 | Method for detecting fumonisins based on immuno-magnetic bead combined enzyme-linked immunosorbent assay |
| CN204608019U (en) * | 2015-04-09 | 2015-09-02 | 余家昌 | A kind of reagent cartridge being provided with magnetic bead transfer organization |
-
2015
- 2015-04-09 CN CN201510165627.7A patent/CN106148184B/en active Active
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| Publication number | Publication date |
|---|---|
| WO2016161739A1 (en) | 2016-10-13 |
| CN106148184A (en) | 2016-11-23 |
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