CN106083796A - A kind of extracting method of vaccinium oxycoccus pigment - Google Patents
A kind of extracting method of vaccinium oxycoccus pigment Download PDFInfo
- Publication number
- CN106083796A CN106083796A CN201610441777.0A CN201610441777A CN106083796A CN 106083796 A CN106083796 A CN 106083796A CN 201610441777 A CN201610441777 A CN 201610441777A CN 106083796 A CN106083796 A CN 106083796A
- Authority
- CN
- China
- Prior art keywords
- vaccinium oxycoccus
- oxycoccus pigment
- extract
- supernatant
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000049 pigment Substances 0.000 title claims abstract description 50
- 241000727741 Vaccinium oxycoccos Species 0.000 title claims abstract description 49
- 235000002118 Vaccinium oxycoccus Nutrition 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 42
- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims abstract description 26
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims abstract description 26
- 235000021014 blueberries Nutrition 0.000 claims abstract description 26
- 240000000851 Vaccinium corymbosum Species 0.000 claims abstract description 24
- 239000000284 extract Substances 0.000 claims abstract description 22
- 239000011347 resin Substances 0.000 claims abstract description 14
- 229920005989 resin Polymers 0.000 claims abstract description 14
- 108010059892 Cellulase Proteins 0.000 claims abstract description 8
- 238000010009 beating Methods 0.000 claims abstract description 8
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000006228 supernatant Substances 0.000 claims description 28
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 21
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims description 16
- 229930014669 anthocyanidin Natural products 0.000 claims description 15
- 235000008758 anthocyanidins Nutrition 0.000 claims description 15
- 239000000287 crude extract Substances 0.000 claims description 12
- 239000003480 eluent Substances 0.000 claims description 12
- 238000001179 sorption measurement Methods 0.000 claims description 12
- 239000003643 water by type Substances 0.000 claims description 9
- 230000005684 electric field Effects 0.000 claims description 8
- JEIPFZHSYJVQDO-UHFFFAOYSA-N ferric oxide Chemical compound O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000007423 decrease Effects 0.000 abstract description 3
- 238000004090 dissolution Methods 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 abstract description 2
- 235000008504 concentrate Nutrition 0.000 abstract description 2
- 239000012141 concentrate Substances 0.000 abstract description 2
- 235000014666 liquid concentrate Nutrition 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 5
- 206010027654 Allergic conditions Diseases 0.000 description 4
- 241001062009 Indigofera Species 0.000 description 4
- 235000021028 berry Nutrition 0.000 description 4
- 230000003203 everyday effect Effects 0.000 description 4
- 230000036039 immunity Effects 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- 235000014102 seafood Nutrition 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000003963 antioxidant agent Substances 0.000 description 3
- 230000003078 antioxidant effect Effects 0.000 description 3
- 235000006708 antioxidants Nutrition 0.000 description 3
- 230000033228 biological regulation Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000638 solvent extraction Methods 0.000 description 3
- 238000000194 supercritical-fluid extraction Methods 0.000 description 3
- 244000077233 Vaccinium uliginosum Species 0.000 description 2
- 235000010208 anthocyanin Nutrition 0.000 description 2
- 229930002877 anthocyanin Natural products 0.000 description 2
- 239000004410 anthocyanin Substances 0.000 description 2
- 150000004636 anthocyanins Chemical group 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 230000002427 irreversible effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 208000003464 asthenopia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940055416 blueberry extract Drugs 0.000 description 1
- 235000019216 blueberry extract Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 210000003792 cranial nerve Anatomy 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000002864 food coloring agent Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses the extracting method of a kind of vaccinium oxycoccus pigment, belong to field of natural organic chemistry.The present invention is with blue berry as raw material, and crushing and beating, is that 40% ethanol solution is as extracting solution with mass fraction, and add cellulase, extract under ultrasonic wave added, then be performing centrifugal separation on, precipitation repeats to extract once, after united extraction liquid concentrates, passes through high-pressure pulse electric, promote the dissolution of vaccinium oxycoccus pigment, increase the vaccinium oxycoccus pigment stripping quantity of extracting solution, then through absorption with macroporous adsorbent resin, alcohol is washed, concentrate, lyophilization, thus obtain vaccinium oxycoccus pigment extracting method.Example proves, the present invention operates controlled, and method is unique, decreases production cost, pollution-free so that the vaccinium oxycoccus pigment extraction ratio up to more than 80% prepared, high purity more than 75%, has higher value medical health care and broader practical application area.
Description
Technical field
The invention discloses the extracting method of a kind of vaccinium oxycoccus pigment, belong to field of natural organic chemistry.
Background technology
Anthocyanidin also known as anthocyanidin, is the water-soluble natural pigment that is widely present in plant of a class, belongs to flavonoid
Thing, it is anthocyanin after being combined with glycosidic bond with glucide, is a kind of natural antioxidant.Owing to anthocyanidin is unstable
Fixed, mainly exist with anthocyanin in plant.
Anthocyanidin is as a kind of natural food colour, safe and nontoxic, aboundresources, and has certain nutrition and pharmacology
Effect, has huge application potential at aspects such as food, cosmetic, medicine.Anthocyanidin is that the nutrition of the defying age of pure natural is mended
Filling agent, research proves mankind nowadays and finds maximally effective antioxidant, is the maximally effective antioxidant of current nature.With
Time have research title can vision enhancing, anti-eye strain；Delay cranial nerve old and feeble.Anthocyanidin contained by blue berry is current all plants
In anthocyanidin, function is the most excellent, range of application is the widest, and side effect is minimum, is also the kind that price is the most expensive.Anthocyanidin content reaches
The blueberry extract price of 25% is 5～6 times that content reaches the Semen Vitis viniferae extract of 95%, it is seen that its value free from worldly cares.Blue berry
Root, stem, leaf, peel, sarcocarp all contain anthocyanidin, and is present in the vacuole of blue berry cell.
Research to the extraction of anthocyanidin at present mainly has water to carry or organic solvent extraction, supercritical fluid extraction
Deng.Traditional water carry or solvent extraction method exist due to the too high structure that can destroy anthocyanidin of temperature, affect its biological activity and
The problems such as productivity reduction；Although modern supercritical extraction extraction ratio is high, but because of apparatus expensive, is not suitable for industrialized production.
Strong in order to solve blue berry Seasonal Harvest, and it is difficult to storage, unsalable blue berry had both caused huge because rotting
Economic loss, the problem polluting again environment, what the present invention provided a kind of high-efficiency and economic extracts vaccinium oxycoccus pigment from blue berry
Method, makes full use of vaccinium oxycoccus pigment, expands the application of vaccinium oxycoccus pigment.
Summary of the invention
The technical problem that present invention mainly solves: extracting during anthocyanidin for current traditional method, water carry or
Solvent extraction method can affect its biological activity and productivity due to temperature too high destruction anthocyanidin structure, and supercritical extraction
Though extraction ratio can be improved, but apparatus expensive, cost is high, is difficult to the present situation of industrialized production, it is provided that one with blue berry is
Raw material, crushing and beating, with mass fraction be 40% ethanol solution as extracting solution, and add cellulase, carry under ultrasonic wave added
Taking, then be performing centrifugal separation on, precipitation repeats to extract once, after united extraction liquid concentrates, by high-pressure pulse electric, to two interpolars
Material applies the high voltage of short pulse punching and processes, and makes polar substances move to electrode direction at a high speed under the effect of electric field, utilizes thin
After birth electroporation principle, causes histiocytic irreversible breaking, thus promotes the dissolution of vaccinium oxycoccus pigment, increases extracting solution
Vaccinium oxycoccus pigment stripping quantity, then through absorption with macroporous adsorbent resin, alcohol is washed, and concentrates, lyophilization, thus obtains vaccinium oxycoccus pigment and carry
Access method.The method operation is controlled, and method is unique, decreases production cost, pollution-free so that the vaccinium oxycoccus pigment prepared extracts
Rate up to more than 80%, high purity more than 75%, there is higher value medical health care and broader practical application area.
In order to solve above-mentioned technical problem, the technical solution adopted in the present invention is:
(1) weigh 200～300g blue berry and clean roguing, add crushing and beating 10 in refiner with 200～300mL deionized waters
～15min, it is in 40% ethanol solution by blue berry serosity addition 2～3L mass concentrations, under 50～52 DEG C of waters bath with thermostatic control, adds
30～45g cellulase, and with 200W ultrasonic echography 10～12min, be that 10% hydrochloric acid solution regulates pH extremely by mass concentration
2.0～2.5, extract 2～3h, lixiviating solution inserted in 4000～6000/min centrifuges centrifugal 10～15min, must precipitate and on
Clear liquid, is extracted once by precipitation by aforesaid operations and is collected supernatant again, merges supernatant, by supernatant at 50～55 DEG C,
It is evaporated to the 30～35% of original volume, obtains vaccinium oxycoccus pigment crude extract；
(2) quality added containing mass concentration is 0.1% hydrochloric acid by solid-liquid ratio 1:6～1:7 by above-mentioned vaccinium oxycoccus pigment crude extract is dense
Degree is in 60% ethanol solution, and with 7～8mL/min by high-pressure pulse electric, controlling umber of pulse is 8～10, and electric field intensity is
18～19KV/cm, and mixed liquor is inserted in 4000～6000/min centrifuges centrifugal 10～15min, take supernatant, obtain blue berry
Anthocyanidin two extract；
(3) above-mentioned vaccinium oxycoccus pigment two extract is filled with 2～4 times of column volumes/h flow velocity process the adsorption column of macroporous resin,
Repetition aforesaid operations is to extracting liquid colourless, then is that 70% ethanol solution is washed with 0.1～0.5 times of column volume/h flow velocity by mass concentration
De-by the vaccinium oxycoccus pigment of macroporous resin adsorption, collect eluent, eluent is reduced pressure dense in 50～55 DEG C of vacuum drying ovens
Contracting, obtains vaccinium oxycoccus pigment by concentrated solution lyophilization.
The application process of the present invention is: by solid-to-liquid ratio 1: 50～1: 100, the vaccinium oxycoccus pigment present invention prepared pours temperature into
In boiled water, stir, oral, it is proposed that morning and evening every day one after each meal, take rear fasting seafood, maror, adhere to even
Continue and take 7～14 days, can substantially feel the fine and smooth gloss that skin becomes, enhance the immunity of skin, for easy autopath,
Can be effective against various allergic conditions, effect is the most notable.
The invention has the beneficial effects as follows:
(1) present invention operates controlled, and method is unique, without specific apparatus during preparation, decreases production cost, without dirty
Dye, belongs to environmental protection technique；
(2) present invention passes through high-pressure pulse electric, the material of two interpolars is applied the high voltage process that short pulse rushes so that polar material
Matter is moved to electrode direction at a high speed under the effect of electric field, utilizes electroporation of cell membrane principle, causes histiocytic irreversible
Destroy, thus promote the dissolution of vaccinium oxycoccus pigment, increase the vaccinium oxycoccus pigment stripping quantity of extracting solution, improve extraction ratio；
(3) present invention finally makes the vaccinium oxycoccus pigment extraction ratio up to more than 80% prepared, high purity more than 75%, has
Higher value medical health care and broader practical application area.
Detailed description of the invention
First weigh 200～300g blue berry and clean roguing, add in refiner broken with 200～300mL deionized waters
Blue berry serosity addition 2～3L mass concentrations are in 40% ethanol solution, 50～52 DEG C of waters bath with thermostatic control by making beating 10～15min
Under, add 30～45g cellulase, and with 200W ultrasonic echography 10～12min, be that 10% hydrochloric acid solution is adjusted by mass concentration
Joint pH to 2.0～2.5, extracts 2～3h, is inserted by lixiviating solution and is centrifuged 10～15min in 4000～6000/min centrifuges, and it is heavy to obtain
Form sediment and supernatant, precipitation is extracted once and collected supernatant by aforesaid operations again, merge supernatant, by supernatant 50～55
At DEG C, it is evaporated to the 30～35% of original volume, obtains vaccinium oxycoccus pigment crude extract；Then by above-mentioned vaccinium oxycoccus pigment crude extract
The mass concentration added containing mass concentration is 0.1% hydrochloric acid by solid-liquid ratio 1:6～1:7 is in 60% ethanol solution, with 7～8mL/
Min passes through high-pressure pulse electric, and controlling umber of pulse is 8～10, and electric field intensity is 18～19KV/cm, and is inserted by mixed liquor
4000～6000/min centrifuges are centrifuged 10～15min, take supernatant, obtain vaccinium oxycoccus pigment two extract；Finally by above-mentioned indigo plant
Certain kind of berries anthocyanidin two extract through being filled with the adsorption column of macroporous resin, repeats aforesaid operations to carrying with 2～4 times of column volumes/h flow velocity
Take liquid colourless, then with mass concentration be 70% ethanol solution with 0.1～0.5 times of column volume/h flow velocity eluting by macroporous resin adsorption
Vaccinium oxycoccus pigment, collect eluent, by eluent concentrating under reduced pressure in 50～55 DEG C of vacuum drying ovens, concentrated solution freezing is done
Dry vaccinium oxycoccus pigment.
Example 1
First weigh 200g blue berry and clean roguing, add crushing and beating 10min in refiner with 200mL deionized water, by indigo plant
It is in 40% ethanol solution that certain kind of berries serosity adds 2L mass concentration, under 50 DEG C of waters bath with thermostatic control, adds 30g cellulase, and uses
200W ultrasonic echography 10min, is 10% hydrochloric acid solution regulation pH to 2.0 by mass concentration, extracts 2h, inserted by lixiviating solution
In 4000/min centrifuge, centrifugal 10min, must precipitate and supernatant, precipitation extracted once by aforesaid operations again and collect supernatant
Liquid, merges supernatant, by supernatant at 50 DEG C, is evaporated to the 30% of original volume, obtains vaccinium oxycoccus pigment crude extract；Then
The mass concentration added containing mass concentration is 0.1% hydrochloric acid by solid-liquid ratio 1:6 by above-mentioned vaccinium oxycoccus pigment crude extract is 60% ethanol
In solution, with 7mL/min by high-pressure pulse electric, controlling umber of pulse is 8, and electric field intensity is 18KV/cm, and by mixed liquor
Insert centrifugal 10min in 4000/min centrifuge, take supernatant, obtain vaccinium oxycoccus pigment two extract；Finally by above-mentioned blue berry cyanine
Element two extracts through being filled with the adsorption column of macroporous resin, repeat aforesaid operations to extracting liquid colourless with 2 times of column volumes/h flow velocity,
It is 70% ethanol solution with 0.1 times of column volume/h flow velocity eluting by the vaccinium oxycoccus pigment of macroporous resin adsorption by mass concentration again, receives
Collection eluent, by eluent concentrating under reduced pressure in 50 DEG C of vacuum drying ovens, obtains vaccinium oxycoccus pigment by concentrated solution lyophilization.
This example operation is easy, and during use, by solid-to-liquid ratio 1: 50, the vaccinium oxycoccus pigment present invention prepared pours warm water into
In, stir, oral, it is proposed that morning and evening every day one after each meal, take rear fasting seafood, maror, adhere to taking continuously
With 7 days, can substantially feel the fine and smooth gloss that skin becomes, enhance the immunity of skin, for easy autopath, can be effective
Resisting various allergic conditions, effect is the most notable.
Example 2
First weigh 250g blue berry and clean roguing, add crushing and beating 13min in refiner with 250mL deionized water, by indigo plant
It is in 40% ethanol solution that certain kind of berries serosity adds 2.5L mass concentration, under 51 DEG C of waters bath with thermostatic control, adds 40g cellulase, and uses
200W ultrasonic echography 11min, is 10% hydrochloric acid solution regulation pH to 2.3 by mass concentration, extracts 2.5h, inserted by lixiviating solution
In 5000/min centrifuge, centrifugal 13min, must precipitate and supernatant, precipitation extracted once by aforesaid operations again and collect supernatant
Liquid, merges supernatant, by supernatant at 53 DEG C, is evaporated to the 33% of original volume, obtains vaccinium oxycoccus pigment crude extract；Then
The mass concentration added containing mass concentration is 0.1% hydrochloric acid by solid-liquid ratio 1:65 by above-mentioned vaccinium oxycoccus pigment crude extract is 60% ethanol
In solution, with 7.5mL/min by high-pressure pulse electric, controlling umber of pulse is 9, electric field intensity 18.5KV/cm, and will mixing
Liquid inserts centrifugal 13min in 5000/min centrifuge, takes supernatant, obtains vaccinium oxycoccus pigment two extract；Finally by above-mentioned blue berry flower
Blue or green plain two extracts with 3 times of column volumes/h flow velocity through being filled with the adsorption column of macroporous resin, repeat aforesaid operations to extracting solution without
Color, then with mass concentration be 70% ethanol solution with 0.4 times of column volume/h flow velocity eluting by the blue berry cyanine of macroporous resin adsorption
Element, collects eluent, by eluent concentrating under reduced pressure in 53 DEG C of vacuum drying ovens, concentrated solution lyophilization is obtained blue berry cyanine
Element.
This example operation is easy, and during use, by solid-to-liquid ratio 1: 80, the vaccinium oxycoccus pigment present invention prepared pours warm water into
In, stir, oral, it is proposed that morning and evening every day one after each meal, take rear fasting seafood, maror, adhere to taking continuously
With 12 days, can substantially feel the fine and smooth gloss that skin becomes, enhance the immunity of skin, for easy autopath, Ke Yiyou
Effect resists various allergic conditions, and effect is the most notable.
Example 3
First weigh 300g blue berry and clean roguing, add crushing and beating 15min in refiner with 300mL deionized water, by indigo plant
It is in 40% ethanol solution that certain kind of berries serosity adds 3L mass concentration, under 52 DEG C of waters bath with thermostatic control, adds 45g cellulase, and uses
200W ultrasonic echography 12min, is 10% hydrochloric acid solution regulation pH to 2.5 by mass concentration, extracts 3h, inserted by lixiviating solution
In 6000/min centrifuge, centrifugal 15min, must precipitate and supernatant, precipitation extracted once by aforesaid operations again and collect supernatant
Liquid, merges supernatant, by supernatant at 55 DEG C, is evaporated to the 35% of original volume, obtains vaccinium oxycoccus pigment crude extract；Then
The mass concentration added containing mass concentration is 0.1% hydrochloric acid by solid-liquid ratio 1:7 by above-mentioned vaccinium oxycoccus pigment crude extract is 60% ethanol
In solution, with 8mL/min by high-pressure pulse electric, controlling umber of pulse is 10, and electric field intensity is 19KV/cm, and will mixing
Liquid inserts centrifugal 15min in 6000/min centrifuge, takes supernatant, obtains vaccinium oxycoccus pigment two extract；Finally by above-mentioned blue berry flower
Blue or green plain two extracts with 4 times of column volumes/h flow velocity through being filled with the adsorption column of macroporous resin, repeat aforesaid operations to extracting solution without
Color, then with mass concentration be 70% ethanol solution with 0.5 times of column volume/h flow velocity eluting by the blue berry cyanine of macroporous resin adsorption
Element, collects eluent, by eluent concentrating under reduced pressure in 55 DEG C of vacuum drying ovens, concentrated solution lyophilization is obtained blue berry cyanine
Element.
This example operation is easy, and during use, by solid-to-liquid ratio 1: 100, the vaccinium oxycoccus pigment present invention prepared is poured temperature into and opened
In water, stir, oral, it is proposed that morning and evening every day one after each meal, take rear fasting seafood, maror, adhere to continuously
Take 14 days, can substantially feel the fine and smooth gloss that skin becomes, enhance the immunity of skin, for easy autopath, permissible
Being effective against various allergic conditions, effect is the most notable.
Claims (1)
1. the extracting method of a vaccinium oxycoccus pigment, it is characterised in that concrete preparation process is:
(1) weigh 200～300g blue berry and clean roguing, add crushing and beating 10 in refiner with 200～300mL deionized waters
～15min, it is in 40% ethanol solution by blue berry serosity addition 2～3L mass concentrations, under 50～52 DEG C of waters bath with thermostatic control, adds
30～45g cellulase, and with 200W ultrasonic echography 10～12min, be that 10% hydrochloric acid solution regulates pH extremely by mass concentration
2.0～2.5, extract 2～3h, lixiviating solution inserted in 4000～6000/min centrifuges centrifugal 10～15min, must precipitate and on
Clear liquid, is extracted once by precipitation by aforesaid operations and is collected supernatant again, merges supernatant, by supernatant at 50～55 DEG C,
It is evaporated to the 30～35% of original volume, obtains vaccinium oxycoccus pigment crude extract；
(2) quality added containing mass concentration is 0.1% hydrochloric acid by solid-liquid ratio 1:6～1:7 by above-mentioned vaccinium oxycoccus pigment crude extract is dense
Degree is in 60% ethanol solution, and with 7～8mL/min by high-pressure pulse electric, controlling umber of pulse is 8～10, and electric field intensity is
18～19KV/cm, and mixed liquor is inserted in 4000～6000/min centrifuges centrifugal 10～15min, take supernatant, obtain blue berry
Anthocyanidin two extract；
(3) above-mentioned vaccinium oxycoccus pigment two extract is filled with 2～4 times of column volumes/h flow velocity process the adsorption column of macroporous resin,
Repetition aforesaid operations is to extracting liquid colourless, then is that 70% ethanol solution is washed with 0.1～0.5 times of column volume/h flow velocity by mass concentration
De-by the vaccinium oxycoccus pigment of macroporous resin adsorption, collect eluent, eluent is reduced pressure dense in 50～55 DEG C of vacuum drying ovens
Contracting, obtains vaccinium oxycoccus pigment by concentrated solution lyophilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610441777.0A CN106083796A (en) | 2016-06-20 | 2016-06-20 | A kind of extracting method of vaccinium oxycoccus pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610441777.0A CN106083796A (en) | 2016-06-20 | 2016-06-20 | A kind of extracting method of vaccinium oxycoccus pigment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106083796A true CN106083796A (en) | 2016-11-09 |
Family
ID=57237137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610441777.0A Pending CN106083796A (en) | 2016-06-20 | 2016-06-20 | A kind of extracting method of vaccinium oxycoccus pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106083796A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107411088A (en) * | 2017-06-20 | 2017-12-01 | 哈尔滨工业大学 | Special blueberry anthocyanin complex tablet of a kind of the elderly and preparation method thereof |
| CN108576142A (en) * | 2018-05-21 | 2018-09-28 | 遵义盛林农业发展有限公司 | A kind of production method of blueberry biscuit |
| CN108719395A (en) * | 2018-05-21 | 2018-11-02 | 遵义盛林农业发展有限公司 | A kind of production method for blueberry biscuit |
| CN108991197A (en) * | 2018-07-27 | 2018-12-14 | 贵州省普定县黔龙茶业有限公司 | A kind of processing method rich in anthocyanidin instant tea |
| CN109293618A (en) * | 2018-11-30 | 2019-02-01 | 黑龙江绿知都生物科技开发有限公司 | A kind of blueberry anthocyanin isolation and purification method |
| CN109553599A (en) * | 2019-01-17 | 2019-04-02 | 江苏省农业科学院 | A kind of blueberry anthocyanin and its preparation method and application |
| CN109793236A (en) * | 2019-04-03 | 2019-05-24 | 海南松吉云商科技有限公司 | A kind of preparation method of Noni extract |
| CN111418742A (en) * | 2020-05-28 | 2020-07-17 | 山西纳安生物科技股份有限公司 | Preparation method of wild cherry juice |
| CN112042846A (en) * | 2020-09-16 | 2020-12-08 | 深圳鼎盛和兴科技有限公司 | Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof |
| CN115381012A (en) * | 2022-09-07 | 2022-11-25 | 黑龙江敬众堂生物科技有限公司 | Preparation method of beverage rich in purple corn anthocyanin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103073532A (en) * | 2012-11-28 | 2013-05-01 | 北京林业大学 | Processing method for increasing blueberry anthocyanidin content and purity |
| CN103435588A (en) * | 2013-09-12 | 2013-12-11 | 南京泽朗农业发展有限公司 | Novel method for preparing blueberry anthocyanidin |
| CN105175380A (en) * | 2015-08-14 | 2015-12-23 | 北京中农弘科生物技术有限公司 | Method for preparing Yunnan pine bark procyanidine |
-
2016
- 2016-06-20 CN CN201610441777.0A patent/CN106083796A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103073532A (en) * | 2012-11-28 | 2013-05-01 | 北京林业大学 | Processing method for increasing blueberry anthocyanidin content and purity |
| CN103435588A (en) * | 2013-09-12 | 2013-12-11 | 南京泽朗农业发展有限公司 | Novel method for preparing blueberry anthocyanidin |
| CN105175380A (en) * | 2015-08-14 | 2015-12-23 | 北京中农弘科生物技术有限公司 | Method for preparing Yunnan pine bark procyanidine |
Non-Patent Citations (1)
| Title |
|---|
| 于东 等: "花色苷提取、分离纯化及鉴定的研究进展", 《食品与发酵工业》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107411088A (en) * | 2017-06-20 | 2017-12-01 | 哈尔滨工业大学 | Special blueberry anthocyanin complex tablet of a kind of the elderly and preparation method thereof |
| CN108576142A (en) * | 2018-05-21 | 2018-09-28 | 遵义盛林农业发展有限公司 | A kind of production method of blueberry biscuit |
| CN108719395A (en) * | 2018-05-21 | 2018-11-02 | 遵义盛林农业发展有限公司 | A kind of production method for blueberry biscuit |
| CN108991197A (en) * | 2018-07-27 | 2018-12-14 | 贵州省普定县黔龙茶业有限公司 | A kind of processing method rich in anthocyanidin instant tea |
| CN109293618B (en) * | 2018-11-30 | 2022-06-17 | 黑龙江绿知都生物科技开发有限公司 | Blueberry anthocyanin separation and purification method |
| CN109293618A (en) * | 2018-11-30 | 2019-02-01 | 黑龙江绿知都生物科技开发有限公司 | A kind of blueberry anthocyanin isolation and purification method |
| CN109553599A (en) * | 2019-01-17 | 2019-04-02 | 江苏省农业科学院 | A kind of blueberry anthocyanin and its preparation method and application |
| CN109793236A (en) * | 2019-04-03 | 2019-05-24 | 海南松吉云商科技有限公司 | A kind of preparation method of Noni extract |
| CN109793236B (en) * | 2019-04-03 | 2022-04-08 | 海南松吉云商科技有限公司 | Preparation method of noni extract |
| CN111418742A (en) * | 2020-05-28 | 2020-07-17 | 山西纳安生物科技股份有限公司 | Preparation method of wild cherry juice |
| CN112042846A (en) * | 2020-09-16 | 2020-12-08 | 深圳鼎盛和兴科技有限公司 | Sea-buckthorn solid beverage for enhancing immunity and inhibiting tumors and preparation method thereof |
| CN115381012A (en) * | 2022-09-07 | 2022-11-25 | 黑龙江敬众堂生物科技有限公司 | Preparation method of beverage rich in purple corn anthocyanin |
| CN115381012B (en) * | 2022-09-07 | 2024-02-09 | 黑龙江敬众堂生物科技有限公司 | Preparation method of beverage rich in purple corn anthocyanin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106083796A (en) | A kind of extracting method of vaccinium oxycoccus pigment | |
| CN102732050B (en) | Method for preparing gardenia pigments from Gardenia jasminoides Ellis | |
| CN102924423B (en) | Method for extracting cyanidin from banana peel | |
| CN101704867B (en) | Method for preparing naringin or hesperidin | |
| CN103342692A (en) | Method for extracting blueberry anthocyanin | |
| CN101531652A (en) | Novel method for extracting anthocyanin in bilberry by double water phases | |
| CN113896754B (en) | Industrial production method for extracting and purifying baicalin from radix scutellariae | |
| CN1854149A (en) | Extraction of Momordica grosvenori beet aglucone V | |
| CN105713716A (en) | Method for extracting sea buckthorn fruit oil in grading mode through combination of aqueous enzymatic method and ultrasonic assistance | |
| CN102604424A (en) | Method for extracting anthocyanins from purple sweet potato | |
| CN106800586A (en) | A kind of method of Moringa protein high efficiency extraction | |
| CN106243172A (en) | A kind of method extracting high-purity black Fructus Lycii anthocyanin | |
| CN105237654A (en) | Method for extracting pectin from orange peel | |
| CN108690099A (en) | A method of the extraction separation procyanidine from black fruit fructus lycii | |
| CN107652337B (en) | Method for extracting and preparing anthocyanin such as black rice, black beans, purple potatoes, roses, peonies and the like | |
| CN113637038A (en) | Method for extracting sweet tea glycoside and sweet tea polyphenol without bitter taste from sweet tea leaves | |
| CN110892987A (en) | Preparation method of wolfberry syrup | |
| CN108530411A (en) | A method of the extraction and purification anthocyanidin from wild cherry certain kind of berries slag | |
| CN101012256A (en) | Method of extracting natural coloring matter anthocyanins in black rice | |
| CN107629879A (en) | The method that sugar orange cull fruit extracts Panongchus citri | |
| CN103349850B (en) | A kind of method for extracting active ingredient in wax-apple | |
| CN106038821A (en) | Method for extracting total flavonoids from boxthorn leaves | |
| CN107056741A (en) | A kind of preparation technology of Application of Mulberry Anthocyanins powder | |
| CN104974124A (en) | Method for extracting procyanidine from dahurian larch barks | |
| CN106279385A (en) | A kind of protein separating method of sunflower seed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161109 |
|
| RJ01 | Rejection of invention patent application after publication |