CN106072574A - A kind of composite plant ferment and its preparation method and application - Google Patents
A kind of composite plant ferment and its preparation method and application Download PDFInfo
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- 239000002131 composite material Substances 0.000 title claims abstract description 56
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 241000196324 Embryophyta Species 0.000 claims abstract description 59
- 102000004190 Enzymes Human genes 0.000 claims abstract description 52
- 108090000790 Enzymes Proteins 0.000 claims abstract description 52
- 235000013339 cereals Nutrition 0.000 claims abstract description 41
- 210000000582 semen Anatomy 0.000 claims abstract description 17
- 101100381997 Danio rerio tbc1d32 gene Proteins 0.000 claims abstract description 16
- 101100381999 Mus musculus Tbc1d32 gene Proteins 0.000 claims abstract description 16
- 235000021329 brown rice Nutrition 0.000 claims abstract description 15
- 239000006041 probiotic Substances 0.000 claims abstract description 15
- 235000018291 probiotics Nutrition 0.000 claims abstract description 15
- 235000003935 Hippophae Nutrition 0.000 claims abstract description 10
- 241000229143 Hippophae Species 0.000 claims abstract description 10
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- 235000016954 Ribes hudsonianum Nutrition 0.000 claims abstract description 10
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- 240000000851 Vaccinium corymbosum Species 0.000 claims abstract description 10
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 10
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- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 14
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- HBGGXOJOCNVPFY-UHFFFAOYSA-N diisononyl phthalate Chemical compound CC(C)CCCCCCOC(=O)C1=CC=CC=C1C(=O)OCCCCCCC(C)C HBGGXOJOCNVPFY-UHFFFAOYSA-N 0.000 description 1
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- AIONOLUJZLIMTK-UHFFFAOYSA-N hesperetin Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(O)=CC(O)=C2C(=O)C1 AIONOLUJZLIMTK-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of composite plant ferment and its preparation method and application.Described composite plant ferment, by fresh fruits such as the miscellaneous grain crops such as Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi and Fructus Rosae Normalis, Fructus Caricae, Fructus Mori, black currant, blue berry, Fructus Hippophae and Fructus Crataegis, obtains through PROCESS FOR TREATMENT such as enzymolysis, probiotics fermention, lyophilizations.Described composite plant ferment steady quality, rich in the organized enzyme having needed for multiple body metabolism, and the nutrient substance such as flavonoid, vitamin, mineral, trace element, and sweet and sour taste, delicate fragrance overflows, nutritional labeling is easily absorbed by the body, and has multiple nutrients health value, is particularly suited for the capillary hemorrhage disease that treatment of vascular fragility increase causes.Composite plant ferment preparation technology of the present invention is simple, and product property is stable, with short production cycle, has important Development volue.
Description
Technical field
The invention belongs to ferment preparation field, be specifically related to a kind of composite plant ferment and its preparation method and application.
Background technology
Ferment is the general name of all enzyme materials, is the active macromolecules produced by organism with catalytic action.
Various biochemical reactions in organism, nearly all to be carried out under the catalytic action of ferment, in health ferment number with
Its activity height, and the aging of human body and disease closely bound up.Modern study shows, ferment is various physiology merits in biosis
Total performance of energy, disease has direct relation with content and the specific ferment shortage of internal metabolism ferment.It is to pass through that ferment is cured the disease
Regulation human body metabolic balance, balanced nutritious, improve internal milieu, thus improve immunity of organisms, exciting human self-healing function with
Vitality reaches the purpose prevented and cured diseases.
And modern society's dog-eat-dog, rhythm of life quickening, environmental pollution, age growth, all can cause the ferment in human body
Element reduces.When human body endo enzyme effect is weak or reduces, just have various symptom and occur.Vascular fragility increase is that evaluation is hemorrhage inclines
To important indicator, be that the diseases such as cardiovascular disease, hypertension, diabetes, dermatosis, rheumatism, liver cirrhosis, gastric ulcer are common
Complication, low closely related with internal aqueous enzyme pancake, the especially shortage with Citrin is closely connected.Citrin be by
The compositions such as bioflavonoids, rutacultin and hesperetin are constituted, and have potent non-oxidizability, are maintaining the blood system normally side of work
Mask has special role, can not only keep the pliability of blood vessel wall, reduces vascular fragility, moreover it is possible to improve the activity of erythrocyte,
Increase oxygen carrier amount and metabolite, effectively suppress PA.Citrin belongs to water soluble vitamins, and human body cannot self close
Becoming, it is necessary to absorb from food, according to relevant information, people from whole world is general lack of Citrin, and its main cause is diet structure
Unreasonable cause.Take ferment and not only can absorb multiple nutritional components, keep balanced in nutrition, and absorption of human body degree is relatively
Height, can make full use of nutrient.
At present, ferment on the market is many based on natural fermentation, effective ingredient not only can not be made to be fully dissolved out, cause raw material
Waste, and be easy to cause other living contaminantses, additionally, the cycle of natural fermentation is the longest, the generally 2-4 month, it is impossible to meet
The market demand.Chinese patent application 201210216324.X discloses a kind of Plant essence enzyme and preparation method thereof, described
Plant essence enzyme by 20 kinds of fresh fruits including Fructus Mali pumilae, Herba Apii graveolentis 10 kinds of interior fresh vegetabless, sugar, trace element,
The raw materials such as egg albumen powder, wine, water are cultivated through 4 oxygenation aerations and are prepared from, and the cycle that each oxygenation aeration is cultivated is 10～18
My god, the production cycle is longer.
Summary of the invention
For solving the problem that prior art exists, the present invention provides a kind of composite plant ferment and preparation method thereof and answers
With.Described composite plant ferment rich in the organized enzyme having needed for multiple body metabolism, and flavonoid, vitamin, mineral,
The nutrient substance such as trace element, and sweet and sour taste, delicate fragrance overflows, and nutritional labeling is easily absorbed by the body, and has multiple nutrients and protects
Strong value, is particularly suited for the capillary hemorrhage disease that treatment of vascular fragility increase causes.Composite plant ferment of the present invention
Element preparation technology is simple, and product property is stable, with short production cycle, has important Development volue.
The present invention is by following technical solution to be attained in that
The preparation method of a kind of composite plant ferment comprises the following steps:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 150～200 mesh sieves, obtain miscellaneous
Grain powder, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 6～7, adds
Compound enzyme, carries out enzymolysis 6～8h under the conditions of 50～55 DEG C, then heats to 100 DEG C of insulations 10～15min stopping enzymolysis, room
Cool down under the conditions of temperature, be then centrifuged for, respectively obtain supernatant and miscellaneous grain crops solid content, standby；
(2), after the miscellaneous grain crops solid content that step (1) obtains being carried out ozone sterilization, add in the ratio that inoculum concentration is 4～6%wt
Entering saccharomyces carlsbergensis bacterium, 30～35 DEG C of lucifuges carry out fermenting 8～10 days, filter, take supernatant and i.e. obtain feed liquid A；
(3) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, by solid-liquid ratio be
The ratio of 1:5 adds water, is subsequently adding 0.01%wt Sal, stirs, obtain pulp serosity, pulp serosity is warming up to 45
～55 DEG C, it is subsequently adding mixed enzyme insulation and carries out enzymolysis, when the soluble solid in pulp serosity is 30～35%, heat up
To 100 DEG C of insulation 10～15min stopping enzymolysis, obtain feed liquid B；
(4) the supernatant mixing that feed liquid B step (3) obtained and step (1) obtain, adds 10%wt sugar, and stirring is all
Even, add saccharomyces carlsbergensis bacterium in ratio that inoculum concentration is 8～10%wt, 30～35 DEG C of lucifuges carry out fermenting 3～5 days, obtain the
One fermentation liquid；
(5) the feed liquid A mixing that the first fermentation liquid step (4) obtained and step (2) obtain, stirs, by inoculation
Amount is the ratio addition compound probiotic of 8～10%wt, and 28～30 DEG C of lucifuges carry out fermenting 3～5 days, obtain the second fermentation liquid；
(6) the second fermentation liquid that step (5) obtains is carried out lyophilization, obtain composite plant ferment；
Wherein, the weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and the Fructus Tritici aestivi in described step (1) is 2:1:1:
0.6:0.4；
Fruit in described step (3) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 10～25%, Fructus Caricae 10
～20%, Fructus Mori 10～20%, black currant 10～20%, blue berry 8～15%, Fructus Hippophae 8～15% and Fructus Crataegi 10～25%；
Sugar in described step (4) is at least one in dextrinosan, soybean oligo saccharide, oligomeric galactose.
Further, the compound enzyme in described step (1) be 1,4 beta-glucanase and amylase with (0.5～1): (0.2～
0.4) weight ratio composition, described compound enzyme addition is 0.5～1.2g/kg miscellaneous grain crops powder.
Preferably, the compound enzyme in described step (1) is 1,4 beta-glucanase and the amylase weight ratio group with 0.8:0.3
Becoming, described compound enzyme addition is 0.8g/kg miscellaneous grain crops powder.
Further, the mixed enzyme in described step (3) be cellulase and pectase with (1～2): the weight ratio group of 1
Becoming, the addition of mixed enzyme is 0.2～0.6g/L pulp serosity.
Preferably, the mixed enzyme in described step (3) is that cellulase and pectase form with the weight ratio of 1.5:1, mixed
The addition of synthase is 0.5g/L pulp serosity.
Further, the compound probiotic in described step (5) is streptococcus thermophilus and the bacillus acidophilus weight with 1:1
Than composition.
Preferably, the fruit in described step (2) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 20%, kind wood
Melon 10%, Fructus Mori 15%, black currant 15%, blue berry 10%, Fructus Hippophae 10% and Fructus Crataegi 20%.
Correspondingly, invention additionally discloses a kind of composite plant ferment, use above-mentioned preparation method to be prepared from.
Correspondingly, the invention also discloses the application of described composite plant ferment, the most described composite plant ferment exists
Preparation treatment of vascular fragility increase causes the application in the health product of capillary hemorrhage disease.
Compared with prior art, present invention have an advantage that
(1) concentrating carry out fermenting different from after the ownership being mixed for raw material in traditional zymotic technique, first the present invention will
Miscellaneous grain crops carry out enzymolysis, produce more nutrient substance, then carry out culture propagation and produce the higher alcohols of concentration, then with fruit
The first fermentation liquid obtained through enzymolysis and fermentation as primary raw material mixes, and forms the substrate of second time fermentation, utilizes dense
Spend higher alcohols and the organic acid reaction of the first fermentation liquid generation, generate more aromatic ester material, reduce acidity simultaneously,
Taste is better to make ferment.
(2) composite plant ferment of the present invention to prepare material combination reasonable, produce collaborative work between each composition
The materials such as abundant dietary fiber, starch based, protide are contained, through enzymolysis, fermentation etc. with, miscellaneous grain crops such as described Semen Fagopyri Esculenti, Herba bromi japonici
After PROCESS FOR TREATMENT, be converted into easily be absorbed by the body aminoacid, polysaccharide, the material such as oligosaccharide, and as fermentation substrate and fruit
Class raw material mixes, and becomes the raw material of submerged fermentation, reduces the viscosity of fruit fermentation.
(3) the composite plant ferment that the present invention provides is rich in there being various trace elements, and containing higher bioflavonoids
Content (Citrin) and superoxide dismutase (SOD) enzyme are lived, and have multiple nutrients health value, are particularly suited for preventing and treating
The capillary hemorrhage disease that vascular fragility increase causes.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following example.
Embodiment 1
The preparation method of the embodiment of the present invention 1 composite plant ferment comprises the following steps:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 200 mesh sieves, obtain coarse cereal powder
End, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 6, adds compound enzyme,
Described compound enzyme is that 1,4 beta-glucanase and amylase form with the weight ratio of 0.8:0.3, and compound enzyme addition is 0.8g/kg miscellaneous grain crops
Powder, carries out enzymolysis 6h under the conditions of 50 DEG C, then heats to 100 DEG C of insulation 10min and stops enzymolysis, cools down under room temperature condition,
It is then centrifuged for, respectively obtains supernatant and miscellaneous grain crops solid content, standby；
(2), after the miscellaneous grain crops solid content that step (1) obtains being carried out ozone sterilization, add in the ratio that inoculum concentration is 5%wt
Saccharomyces carlsbergensis bacterium, 30 DEG C of lucifuges carry out fermenting 8 days, filter, take supernatant and i.e. obtain feed liquid A；
(3) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, by solid-liquid ratio be
The ratio of 1:5 adds water, is subsequently adding 0.01%wt Sal, stirs, obtain pulp serosity, pulp serosity is warming up to 45
DEG C, it being subsequently adding mixed enzyme insulation and carry out enzymolysis, described mixed enzyme is cellulase and the pectase weight ratio group with 1.5:1
Becoming, the addition of mixed enzyme is 0.5g/L pulp serosity, when the soluble solid in pulp serosity is 35%, is warming up to
100 DEG C of insulation 10min stop enzymolysis, obtain feed liquid B；
(4) the supernatant mixing that feed liquid B step (3) obtained and step (1) obtain, adds 10%wt soy oligosaccharides
Sugar, stirs, and adds saccharomyces carlsbergensis bacterium in the ratio that inoculum concentration is 8%wt, and 30 DEG C of lucifuges carry out fermenting 5 days, obtain first
Fermentation liquid；
(5) the feed liquid A mixing that the first fermentation liquid step (4) obtained and step (2) obtain, stirs, by inoculation
The ratio that amount is 8%wt adds compound probiotic, and described compound probiotic is streptococcus thermophilus and the bacillus acidophilus weight with 1:1
Amount is than composition, and 30 DEG C of lucifuges carry out fermenting 5 days, obtain the second fermentation liquid；
(6) the second fermentation liquid that step (5) obtains is carried out lyophilization, obtain composite plant ferment；
Wherein, the weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and the Fructus Tritici aestivi in described step (1) is 2:1:1:
0.6:0.4；
Fruit in described step (3) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 20%, Fructus Caricae 10%,
Fructus Mori 15%, black currant 15%, blue berry 10%, Fructus Hippophae 10% and Fructus Crataegi 20%.
Embodiment 2
The preparation method of the embodiment of the present invention 2 composite plant ferment comprises the following steps:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 200 mesh sieves, obtain coarse cereal powder
End, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 6, adds compound enzyme,
Described compound enzyme is that 1,4 beta-glucanase and amylase form with the weight ratio of 0.5:0.2, and compound enzyme addition is 0.5g/kg miscellaneous grain crops
Powder, carries out enzymolysis 6h under the conditions of 50 DEG C, then heats to 100 DEG C of insulation 10min and stops enzymolysis, cools down under room temperature condition,
It is then centrifuged for, respectively obtains supernatant and miscellaneous grain crops solid content, standby；
(2), after the miscellaneous grain crops solid content that step (1) obtains being carried out ozone sterilization, add in the ratio that inoculum concentration is 5%wt
Saccharomyces carlsbergensis bacterium, 30 DEG C of lucifuges carry out fermenting 8 days, filter, take supernatant and i.e. obtain feed liquid A；
(3) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, by solid-liquid ratio be
The ratio of 1:5 adds water, is subsequently adding 0.01%wt Sal, stirs, obtain pulp serosity, pulp serosity is warming up to 45
DEG C, it being subsequently adding mixed enzyme insulation and carry out enzymolysis, described mixed enzyme is that cellulase and pectase form with the weight ratio of 2:1,
The addition of mixed enzyme is 0.6g/L pulp serosity, when the soluble solid in pulp serosity is 35%, is warming up to 100 DEG C
Insulation 10min stops enzymolysis, obtains feed liquid B；
(4) the supernatant mixing that feed liquid B step (3) obtained and step (1) obtain, adds 10%wt soy oligosaccharides
Sugar, stirs, and adds saccharomyces carlsbergensis bacterium in the ratio that inoculum concentration is 8%wt, and 30 DEG C of lucifuges carry out fermenting 5 days, obtain first
Fermentation liquid；
(5) the feed liquid A mixing that the first fermentation liquid step (4) obtained and step (2) obtain, stirs, by inoculation
The ratio that amount is 8%wt adds compound probiotic, and described compound probiotic is streptococcus thermophilus and the bacillus acidophilus weight with 1:1
Amount is than composition, and 30 DEG C of lucifuges carry out fermenting 5 days, obtain the second fermentation liquid；
(6) the second fermentation liquid that step (5) obtains is carried out lyophilization, obtain composite plant ferment；
Wherein, the weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and the Fructus Tritici aestivi in described step (1) is 2:1:1:
0.6:0.4；
Fruit in described step (3) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 25%, Fructus Caricae 10%,
Fructus Mori 20%, black currant 10%, blue berry 10%, Fructus Hippophae 15% and Fructus Crataegi 10%.
Embodiment 3
The preparation method of the embodiment of the present invention 3 composite plant ferment comprises the following steps:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 150 mesh sieves, obtain coarse cereal powder
End, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 7, adds compound enzyme,
Described compound enzyme is that 1,4 beta-glucanase and amylase form with the weight ratio of 0.7:0.4, and compound enzyme addition is 1.2g/kg miscellaneous grain crops
Powder, carries out enzymolysis 8h under the conditions of 55 DEG C, then heats to 100 DEG C of insulation 15min and stops enzymolysis, cools down under room temperature condition,
It is then centrifuged for, respectively obtains supernatant and miscellaneous grain crops solid content, standby；
(2), after the miscellaneous grain crops solid content that step (1) obtains being carried out ozone sterilization, add in the ratio that inoculum concentration is 6%wt
Saccharomyces carlsbergensis bacterium, 35 DEG C of lucifuges carry out fermenting 10 days, filter, take supernatant and i.e. obtain feed liquid A；
(3) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, by solid-liquid ratio be
The ratio of 1:5 adds water, is subsequently adding 0.01%wt Sal, stirs, obtain pulp serosity, pulp serosity is warming up to 55
DEG C, it being subsequently adding mixed enzyme insulation and carry out enzymolysis, described mixed enzyme is that cellulase and pectase form with the weight ratio of 1:1,
The addition of mixed enzyme is 0.2g/L pulp serosity, when the soluble solid in pulp serosity is 30%, is warming up to 100 DEG C
Insulation 15min stops enzymolysis, obtains feed liquid B；
(4) the supernatant mixing that feed liquid B step (3) obtained and step (1) obtain, adds 10%wt soy oligosaccharides
Sugar, stirs, and adds saccharomyces carlsbergensis bacterium in the ratio that inoculum concentration is 10%wt, and 35 DEG C of lucifuges carry out fermenting 5 days, obtain first
Fermentation liquid；
(5) the feed liquid A mixing that the first fermentation liquid step (4) obtained and step (2) obtain, stirs, by inoculation
The ratio that amount is 10%wt adds compound probiotic, and described compound probiotic is that streptococcus thermophilus and bacillus acidophilus are with 1:1's
Ratio forms, and 28 DEG C of lucifuges carry out fermenting 5 days, obtain the second fermentation liquid；
(6) the second fermentation liquid that step (5) obtains is carried out lyophilization, obtain composite plant ferment；
Wherein, the weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and the Fructus Tritici aestivi in described step (1) is 2:1:1:
0.6:0.4；
Fruit in described step (3) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 10%, Fructus Caricae 10%,
Fructus Mori 15%, black currant 20%, blue berry 8%, Fructus Hippophae 12% and Fructus Crataegi 25%.
Comparative example 1
This comparative example 1 is with the preparation method difference of the composite plant ferment of embodiment 1, the miscellaneous grain crops that step (1) obtains
Solid content does not carry out culture propagation, carries out second time and ferment after directly mixing with the first fermentation liquid, and concrete operation step is as follows:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 200 mesh sieves, obtain coarse cereal powder
End, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 6, adds compound enzyme,
Described compound enzyme is that 1,4 beta-glucanase and amylase form with the weight ratio of 0.8:0.3, and compound enzyme addition is 0.8g/kg miscellaneous grain crops
Powder, carries out enzymolysis 6h under the conditions of 50 DEG C, then heats to 100 DEG C of insulation 10min and stops enzymolysis, cools down under room temperature condition,
It is then centrifuged for, respectively obtains supernatant and miscellaneous grain crops solid content, standby；
(2) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, by solid-liquid ratio be
The ratio of 1:5 adds water, is subsequently adding 0.01%wt Sal, stirs, obtain pulp serosity, pulp serosity is warming up to 45
DEG C, it being subsequently adding mixed enzyme insulation and carry out enzymolysis, described mixed enzyme is cellulase and the pectase weight ratio group with 1.5:1
Becoming, the addition of mixed enzyme is 0.5g/L pulp serosity, when the soluble solid in pulp serosity is 35%, is warming up to
100 DEG C of insulation 10min stop enzymolysis, obtain feed liquid；
(3) the supernatant mixing that feed liquid step (2) obtained and step (1) obtain, adds 10%wt soybean oligo saccharide,
Stirring, add saccharomyces carlsbergensis bacterium in the ratio that inoculum concentration is 8%wt, 30 DEG C of lucifuges carry out fermenting 5 days, obtain the first fermentation
Liquid；
(4) the miscellaneous grain crops solid content mixing that the first fermentation liquid step (3) obtained and step (1) obtain, stirs, presses
Inoculum concentration is that the ratio of 8%wt adds compound probiotic, and described compound probiotic is that streptococcus thermophilus and bacillus acidophilus are with 1:1
Weight ratio composition, 30 DEG C of lucifuges carry out fermenting 5 days, obtain the second fermentation liquid；
(5) the second fermentation liquid that step (4) obtains is carried out lyophilization, obtain composite plant ferment；
Wherein, the weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and the Fructus Tritici aestivi in described step (1) is 2:1:1:
0.6:0.4；
Fruit in described step (2) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 20%, Fructus Caricae 10%,
Fructus Mori 15%, black currant 15%, blue berry 10%, Fructus Hippophae 10% and Fructus Crataegi 20%.
Comparative example 2
The preparation method of comparative example 2 composite plant ferment of the present invention is substantially the same manner as Example 1, and difference is, described step
Suddenly the pulp serosity in (3) does not use cellulase and pectase to carry out enzymolysis, and the supernatant that direct and step (1) obtains mixes
Closing, concrete operation step is as follows:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 200 mesh sieves, obtain coarse cereal powder
End, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 6, adds compound enzyme,
Described compound enzyme is that 1,4 beta-glucanase and amylase form with the weight ratio of 0.8:0.3, and compound enzyme addition is 0.8g/kg miscellaneous grain crops
Powder, carries out enzymolysis 6h under the conditions of 50 DEG C, then heats to 100 DEG C of insulation 10min and stops enzymolysis, cools down under room temperature condition,
It is then centrifuged for, respectively obtains supernatant and miscellaneous grain crops solid content, standby；
(2), after the miscellaneous grain crops solid content that step (1) obtains being carried out ozone sterilization, add in the ratio that inoculum concentration is 5%wt
Saccharomyces carlsbergensis bacterium, 30 DEG C of lucifuges carry out fermenting 8 days, filter, take supernatant and i.e. obtain feed liquid A；
(3) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, by solid-liquid ratio be
The ratio of 1:5 adds water, is subsequently adding 0.01%wt Sal, stirs, obtains pulp serosity；
(4) the supernatant mixing that pulp serosity step (3) obtained and step (1) obtain, adds 10%wt Semen sojae atricolor low
Polysaccharide, stirs, and adds saccharomyces carlsbergensis bacterium in ratio that inoculum concentration is 8%wt, and 30 DEG C of lucifuges carry out fermenting 5 days, obtains the
One fermentation liquid；
(5) the feed liquid A mixing that the first fermentation liquid step (4) obtained and step (2) obtain, stirs, by inoculation
The ratio that amount is 8%wt adds compound probiotic, and described compound probiotic is streptococcus thermophilus and the bacillus acidophilus weight with 1:1
Amount is than composition, and 30 DEG C of lucifuges carry out fermenting 5 days, obtain the second fermentation liquid；
(6) the second fermentation liquid that step (5) obtains is carried out lyophilization, obtain composite plant ferment；
Wherein, the weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and the Fructus Tritici aestivi in described step (1) is 2:1:1:
0.6:0.4；
Fruit in described step (3) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 20%, Fructus Caricae 10%,
Fructus Mori 15%, black currant 15%, blue berry 10%, Fructus Hippophae 10% and Fructus Crataegi 20%.
The composite plant ferment quality evaluation that test example one, the present invention prepare
(1) composite plant ferment flavor evaluation
The composite plant ferment flavor evaluation that table 1 present invention prepares
As seen from the above table, the present invention prepares embodiment 1-3 the composite plant ferment prepared and answering that comparative example 1 and 2 prepares
Conjunction plant enzyme compares, and has stronger fragrance, and sour-sweet suitably, taste is better.
(2) each component content of composite plant ferment measures
Bioflavonoids (Citrin) content and SOD enzyme in the composite plant ferment preparing the present invention are lived and are carried out
Detection, and use the composite plant that embodiment 1-3 and comparative example 1-2 prepare by inductively coupled plasma mass spectrometry (ICP-MS)
Micronutrient levels in ferment is measured, and the results are shown in Table 2.
Each component content measurement result in the composite plant ferment that table 2 present invention prepares
| Testing index | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 |
| Bioflavonoids (mg/g) | 106.42 | 104.58 | 101.15 | 88.52 | 80.24 |
| SOD enzyme lives (U/g) | 138.25 | 135.46 | 132.37 | 117.57 | 104.83 |
| Ferrum (mg/g) | 0.151 | 0.155 | 0.148 | 0.133 | 0.110 |
| Zinc (mg/g) | 0.645 | 0.637 | 0.622 | 0.515 | 0.413 |
| Manganese (mg/g) | 1.758 | 1.834 | 1.693 | 1.425 | 1.374 |
| Iodine (mg/g) | 0.075 | 0.080 | 0.072 | 0.068 | 0.053 |
| Selenium (mg/g) | 0.468 | 0.472 | 0.450 | 0.360 | 0.282 |
From upper table 2, the composite plant ferment that embodiment of the present invention 1-3 prepares contains various trace elements, and contains
There is more biology total flavones (Citrin) and stronger exceed superoxide dismutase (SOD) activity, showing answering of gained
Conjunction plant enzyme is nutritious, and its abundant Citrin and stronger SOD enzyme are lived and be all conducive to increasing vascular toughness, reduce
The bleeding risk caused because vascular fragility increases.And nutritional labeling that the composite plant ferment of comparative example 1,2 gained contains is equal
Decrease, especially the most obvious with comparative example 2.Therefore, the preparation method of composite plant ferment of the present invention can make ferment
Element is prepared the effective ingredient of raw material and is fully dissolved out, and improves the quality of product.
The composite plant ferment that test example two, the present invention prepare is to hypertension, hyperlipemia, coronary heart disease volunteer patients's capillary
The impact of vascular fragility
According to prescription: composite plant ferment lyophilized powder 30g, microcrystalline Cellulose 50g, lactose 30g, Pulvis Talci 15g, polyvidone
K30 25g, the composite plant ferment lyophilized powder embodiment of the present invention 1,2,3 and comparative example 1,2 prepared is by dry granulation pressure
Sheet legal system piece agent, every contains composite plant ferment lyophilized powder 0.2g.
Screening hypertension, hyperlipemia, each 250 examples of coronary heart disease volunteer patients, be divided into 5 groups, respectively embodiment 1-3 group with
Comparative example 1-2 group, often hypertension, hyperlipemia, each 50 examples of patients with coronary heart disease in group, each group patient takes the present invention every day and implements
The composite plant ferment tablet that example 1,2,3 and comparative example 1,2 prepare, 3 times/day, 4 pieces/times, takes continuously 2 weeks, measures before taking
The capillary fragility of rear volunteer patients, assay method is: uses negative pressure method, i.e. the plastic cup of internal diameter 7 millimeters is overlying on forearm
Bending on the skin of side certain area, this cup has been connected in vial, vacuum table and have on the syringe of piston, and being bled by syringe makes to reach
To certain negative pressure, taking out plastic cup after one minute, under observation cup, skin is with or without petechia.Take out negative pressure typically to open from-200mmHg
Begin, as without petechia, the position of mobile cup the most in certain sequence, then the pressure of incremental-50mmHg, until presenting petechia, with
Presenting the minimum negative pressure needed for petechia is capillary fragility index, and this value the least expression fragility is the highest, blood capillary resists
Power is low.
The standard of curative effect evaluation: 1) take before and after the most hemorrhage count constant, but take rear negative pressure value increase, represent blood capillary crisp
Property reduce；2) before and after's negative pressure value is taken constant, but hemorrhage minimizing of counting after taking, represent that capillary fragility reduces；3) take
Rear negative pressure value relatively takes front increase, and hemorrhage counting is reduced on the contrary, represents that capillary fragility reduces；4) before and after's negative pressure value is taken
Constant, hemorrhage count constant, represent capillary fragility constant；5) take before and after's negative pressure value constant, and take the most hemorrhage counting
Increase on the contrary or the most hemorrhage before and after taking count constant, and taking rear negative pressure value and reduce, all represent that capillary fragility increases.
The results are shown in Table 3.
The composite plant ferment that table 3 present invention prepares impact on capillary fragility
As seen from the above table, the composite plant ferment that embodiment of the present invention 1-3 prepares is to hypertension, hyperlipemia, coronary heart disease
The capillary fragility of volunteer patients all has a better role, and can effectively reduce the capillary fragility of volunteer patients, increases
The effect of elastic resistance of strong blood capillary, reduces hemorrhage tendency, and its therapeutic effect is better than the composite plant ferment that comparative example 1,2 prepares
Element.
Below it is only the preferred embodiment of the present invention, it is noted that it is right that above-mentioned preferred implementation is not construed as
The restriction of the present invention, protection scope of the present invention should be as the criterion with claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, it is also possible to make some improvements and modifications, these change
Enter and retouch and also should be regarded as protection scope of the present invention.
Claims (9)
1. the preparation method of a composite plant ferment, it is characterised in that comprise the following steps:
(1) take Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi grind into powder, cross 150～200 mesh sieves, obtain coarse cereal powder
End, adds water in the ratio that solid-liquid ratio is 1:10 in miscellaneous grain crops powder, uses sodium carbonate liquor regulation pH to 6～7, adds compound
Enzyme, carries out enzymolysis 6～8h under the conditions of 50～55 DEG C, then heats to 100 DEG C of insulations 10～15min stopping enzymolysis, room temperature bar
Cool down under part, be then centrifuged for, respectively obtain supernatant and miscellaneous grain crops solid content, standby；
(2), after the miscellaneous grain crops solid content that step (1) obtains being carried out ozone sterilization, card is added in the ratio that inoculum concentration is 4～6%wt
Family name's yeast, 30～35 DEG C of lucifuges carry out fermenting 8～10 days, filter, take supernatant and i.e. obtain feed liquid A；
(3) take fresh fruit, clean, remove flash removed and kernel, sarcocarp is crushed together with peel, be 1:5 by solid-liquid ratio
Ratio add water, be subsequently adding 0.01%wt Sal, stir, obtain pulp serosity, pulp serosity is warming up to 45～
55 DEG C, it is subsequently adding mixed enzyme insulation and carries out enzymolysis, when the soluble solid in pulp serosity is 30～35%, be warming up to
100 DEG C of insulation 10～15min stopping enzymolysis, obtain feed liquid B；
(4) the supernatant mixing that feed liquid B step (3) obtained and step (1) obtain, adds 10%wt sugar, stirs, press
Inoculum concentration is the ratio addition saccharomyces carlsbergensis bacterium of 8～10%wt, and 30～35 DEG C of lucifuges carry out fermenting 3～5 days, obtain the first fermentation
Liquid；
(5) the feed liquid A mixing that the first fermentation liquid step (4) obtained and step (2) obtain, stirs, is 8 by inoculum concentration
～the ratio of 10%wt adds compound probiotic, 28～30 DEG C of lucifuges carry out fermenting 3～5 days, obtain the second fermentation liquid；
(6) the second fermentation liquid that step (5) obtains is carried out lyophilization, obtain composite plant ferment；
The weight ratio of Semen Fagopyri Esculenti, Herba bromi japonici, brown rice, bran of Radix Et Rhizoma Fagopyri Tatarici and Fructus Tritici aestivi in described step (1) is 2:1:1:0.6:0.4；
Fruit in described step (3) is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 10～25%, Fructus Caricae 10～
20%, Fructus Mori 10～20%, black currant 10～20%, blue berry 8～15%, Fructus Hippophae 8～15% and Fructus Crataegi 10～25%；
Sugar in described step (4) is at least one in dextrinosan, soybean oligo saccharide, oligomeric galactose.
The preparation method of composite plant ferment the most according to claim 1, it is characterised in that answering in described step (1)
Synthase be 1,4 beta-glucanase and amylase with (0.5～1): the weight ratio composition of (0.2～0.4), described compound enzyme addition is
0.5～1.2g/kg miscellaneous grain crops powder.
The preparation method of composite plant ferment the most according to claim 2, it is characterised in that answering in described step (1)
Synthase is that 1,4 beta-glucanase and amylase form with the weight ratio of 0.8:0.3, and described compound enzyme addition is 0.8g/kg coarse cereal powder
End.
The preparation method of composite plant ferment the most according to claim 1, it is characterised in that mixing in described step (3)
Synthase be cellulase and pectase with (1～2): the weight ratio composition of 1, the addition of mixed enzyme is 0.2～0.6g/L pulp
Serosity.
The preparation method of composite plant ferment the most according to claim 4, it is characterised in that mixing in described step (3)
Synthase is that cellulase and pectase form with the weight ratio of 1.5:1, and the addition of mixed enzyme is 0.5g/L pulp serosity.
The preparation method of composite plant ferment the most according to claim 1, it is characterised in that answering in described step (5)
Closing probiotic bacteria is that streptococcus thermophilus and bacillus acidophilus form with the weight ratio of 1:1.
The preparation method of composite plant ferment the most according to claim 1, it is characterised in that the water in described step (3)
Fruit is made up of the raw material of following percentage by weight meter: Fructus Rosae Normalis 20%, Fructus Caricae 10%, Fructus Mori 15%, black currant 15%, blue berry
10%, Fructus Hippophae 10% and Fructus Crataegi 20%.
8. a composite plant ferment, it is characterised in that obtain according to the arbitrary described preparation method of claim 1-7.
9. the composite plant ferment prepared according to the arbitrary described preparation method of claim 1-7 increases in preparation treatment of vascular fragility
Add the application in the health product causing capillary hemorrhage disease.
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| CN110464000A (en) * | 2019-09-11 | 2019-11-19 | 上海应用技术大学 | A kind of compound fruit enzyme liquid and preparation method thereof suitable for bread fermentation |
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| CN107744079B (en) * | 2017-09-28 | 2020-12-25 | 华侨大学 | Preparation method of phyllanthus emblica fermented flavor beverage |
| CN107929133A (en) * | 2017-12-08 | 2018-04-20 | 王颖 | A kind of freckle removing and whitening SOD complex enzyme formulas |
| CN108719983A (en) * | 2018-05-22 | 2018-11-02 | 中南林业科技大学 | A kind of preparation method of preserved egg ferment meal replacement powder |
| CN110140909A (en) * | 2019-05-30 | 2019-08-20 | 重庆康菌泰生物科技股份有限公司 | A kind of Rosa roxburghii Tratt comprehensive enzyme and preparation method thereof |
| CN110463994A (en) * | 2019-08-20 | 2019-11-19 | 南昌大学 | A kind of preparation method of plant enzyme |
| CN110464000A (en) * | 2019-09-11 | 2019-11-19 | 上海应用技术大学 | A kind of compound fruit enzyme liquid and preparation method thereof suitable for bread fermentation |
| CN110810831A (en) * | 2019-12-03 | 2020-02-21 | 安徽妙奇树生物科技有限公司 | An edible ferment prepared from herbal materials and five cereals, and its preparation method |
| CN111035002A (en) * | 2020-01-16 | 2020-04-21 | 肽晟堂生物科技(常州)有限公司 | Preparation method of saponin rice enzyme |
| CN114304620A (en) * | 2021-12-30 | 2022-04-12 | 新疆惠泽食品有限责任公司 | Xinjiang medicine mulberry compound enzyme fermented by compound bacteria and preparation method thereof |
| CN114304620B (en) * | 2021-12-30 | 2023-09-19 | 新疆惠泽食品有限责任公司 | Xinjiang medicine mulberry compound enzyme fermented by compound bacteria and preparation method thereof |
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