CN106071186B - Preparation method of special fermented concentrate silage for sows - Google Patents

Abstract

The invention belongs to the technical field of feed, enzyme preparation and microbial fermentation, and in particular relates to a method for preparing fermented concentrate silage special for sows. Cut fresh pasture or root and tuber into short pieces, beat them, insert lactic acid bacteria, and make silage after natural enzymolysis and anaerobic fermentation; combine one or more of cakes, by-products of food processing, and grains with clean water and molasses Mixing syrup, adding lactic acid bacteria seed solution, natural enzymolysis and anaerobic fermentation, to obtain the wet base of fermented concentrate; adding aerobic fermentation seed liquid to the concentrate silage fermentation substrate, and natural aerobic fermentation to obtain Special fermented concentrate silage for sows. The fermented concentrate silage is rich in fermentable cellulose lactic acid, calcium lactate, small peptides, amino acids, vitamins and probiotics. Increases feed intake and significantly reduces constipation in sows.

Description

Preparation method of special fermented concentrate silage for sows

technical field

The invention belongs to the technical field of feed, enzyme preparation and microbial fermentation, and in particular relates to a method for preparing fermented concentrate silage special for sows.

Background technique

China is a country with a large population and the largest pig raising country in the world. In 2015, the output value of China's animal husbandry industry reached 3.5 trillion yuan, 643 million pigs were slaughtered, of which 45 million sows were on hand, and pork output was 54.87 million tons. According to statistics from the Ministry of Agriculture, the national commercial feed production reached 218 million tons in 2015. Among them, 179 million tons of full-price feed, 21.51 million tons of concentrated feed, 6.4 million tons of premixed feed, including 89.74 million tons of pig feed, 90 million tons of poultry feed, 53 million tons of aquatic feed, and 8.5 million tons of cattle and sheep feed, 3.5 million tons of feed for rabbits, raccoons and pets, and more than 5 million tons of feed for sows nationwide. It is estimated that by 2020, the national compound feed output will reach more than 220 million tons, of which sow feed will reach more than 8 million tons.

As we all know, the quality of feed for lactating sows not only directly affects the milk yield of sows, the survival rate of weaned piglets and the weaning weight of piglets, but also affects the normal estrus and mating of sows after weaning. However, due to the low cellulose content and poor quality of feed cellulose in sows, the milk production of lactating sows in my country is generally low, and the sows are very constipated, which restricts the development of the pig industry. Therefore, it is very important to improve the cellulose content and quality of lactating sow feed.

Silage is lactic acid bacteria fermented feed. Natural silage is fermented by using the lactic acid bacteria present on the plants in nature. Due to the low content of lactic acid bacteria in the natural plants, it only accounts for 0.01-1% of the total number of bacteria. Therefore, during the fermentation process, it is difficult for lactic acid bacteria to quickly form a dominant flora and cannot lower the pH value in a short time. The results: one is that various bacteria are growing and multiplying so that the temperature rises rapidly, causing the pre-fermentation period to be prolonged. The second is that during the preparatory fermentation process, the silage will lose a lot of nutrients and energy due to heat, and also cause a pungent smell and poor palatability. The third is the massive reproduction of mold and spoilage bacteria during the fermentation process, resulting in local mildew and rot of the silage, especially the severe mildew and rot on the top, bottom and edges. Fourth, due to the existence of a large number of miscellaneous bacteria, when the silage is opened, it is easy to form secondary fermentation, and new mold spots or moldy patches will occur on the feeding section. If the situation is not good, it will cause complete mildew and rot. Internationally, exogenous lactic acid bacteria are generally used to improve the quality of silage.

Silage has been widely used in dairy cattle feeding, but its application in sow feeding is just in the ascendant. Feeding experiments show that the cellulose of silage is of high quality, especially the content of neutral detergent fiber, which can stimulate the intake of dairy cows and sows, relieve constipation of sows, and has some functions of fermentable cellulose. Therefore, how to make good use of silage in sow feeding is a topic worthy of research and discussion.

In recent years, fermented feed, as a green and environmentally friendly feed, has become a hot spot in animal nutrition research under the background that the safety of animal products has become the main issue in the development of animal husbandry. Feed fermentation can not only open up feed resources, improve feed palatability, but also improve feed digestibility. It is worth mentioning that the fermented feed has obvious effects in inhibiting the reproduction and growth of Escherichia coli, Salmonella, and some pathogenic bacteria and pathogens in the digestive tract. Therefore, countries around the world have paid more and more attention to fermented feed in recent years. Feed Fermentation technology is in the ascendant. For example: According to statistics, by 2011, 60% of pig farms in Denmark have been using fermented feed, and this number is still rising; the United States and Canada are also vigorously promoting the use of fermented feed; It has also been better developed.

Attempts have long been made to ferment fresh grass or root tubers with soybean meal, bran residue, corn gluten meal, and grains. However, fresh pastures or roots and tubers are particularly prone to nitrite production and are also very susceptible to spoilage, leading to failure of the entire fermentation. Existing studies have shown that: fresh forage or root tubers are more suitable for silage fermentation alone, and there are still technical difficulties in the fermentation of fresh forage or root tubers with soybean meal, bran residue, corn gluten meal and grains, and fresh forage or root tubers have not been seen with Reports or technologies on the successful fermentation of cakes, by-products of food processing, and grains together.

Contents of the invention

In view of the low cellulose content and poor quality of feed cellulose in my country's current sows, which lead to low lactation and severe constipation in sows, and the fermentation of fresh pasture or root tubers with cakes, food processing by-products, and grains will produce nitrous acid For the problem of salt and spoilage, the purpose of the present invention is to provide a kind of fermentable cellulose, lactic acid, calcium lactate, small peptides, amino acids, vitamins and probiotics, which are rich in amino acids and balanced, high in nutrition, easy to digest, and palatable. Good features, can significantly increase sow lactation, significantly increase feed intake, and significantly reduce sow constipation special fermented concentrate silage for sows.

The technical scheme adopted in the present invention is:

A method for preparing fermented concentrate silage special for sows, the steps are as follows:

①. Preparation of silage:

Cut fresh grass and/or root tubers into short pieces, beat them, mix them with lactic acid bacteria seed solution and compound enzyme, fill and compact them in silo or tower, and make silage after natural enzymatic hydrolysis and anaerobic fermentation;

②. Preparation of fermented concentrate wet base:

After mixing one or more of cakes, by-products of food processing, and grains with clean water and molasses syrup, they are then mixed with lactic acid bacteria seed liquid and compound enzymes, and after natural enzymatic hydrolysis and anaerobic fermentation, the wet base of fermented concentrates is obtained ;

③. Preparation of concentrate silage fermentation substrate:

After mixing silage, fermented concentrate wet base and stone powder, the concentrate silage fermentation substrate is prepared;

④. Preparation of fermented concentrate silage for sows:

Add the aerobic fermentation strain solution to the concentrate silage fermentation substrate, and after natural aerobic fermentation, the special fermented concentrate silage wet base for sows is obtained, or the special fermented concentrate silage for sows is obtained after drying Feed dry basis.

Further, the fresh forage and/or root and tuber are one or more of the fresh forage and/or root and tuber feed in the feed material catalog of the Ministry of Agriculture, such as silage corn, alfalfa grass, clover, water hyacinth, water duckweed , water peanuts, amaranth grains, sweet potatoes, carrots, cassava, sweet potatoes, pumpkins, potatoes in one or more;

The cake is one or more of the cake feed in the feed raw material catalog of the Ministry of Agriculture, including soybean meal, rapeseed meal, cotton meal, peanut meal, linseed meal, sunflower seed meal, rice bran meal, corn germ meal, Or one or more of the above-mentioned puffed cake products;

The by-products of food processing are one or more of the by-products of food processing in the feed raw material catalog of the Ministry of Agriculture, including bran, rice bran, wheat gluten, corn gluten powder, rice gluten powder, DDGS, vinegar residue, distiller's grains, One or more of beer grains and starch residue;

The grain is one or more of the grain products in the feed raw material catalog of the Ministry of Agriculture, corn, rice, broken rice, wheat, flour, oats, barley, sorghum, or one of the puffed products of the above grains or more;

The molasses syrup is one or more of the molasses syrup in the feed raw material catalog of the Ministry of Agriculture, including sucrose syrup/powder, glucose syrup/powder, fructose syrup/powder, maltodextrin pulp/powder, fructose syrup/powder, One or more of maltose syrup/powder, oligosaccharide syrup/powder, sucrose molasses, beet molasses, soybean molasses.

Preferably, the fresh pasture and/or roots and tubers are one or more of silage corn, alfalfa, sweet potato, carrot, and pumpkin;

The cake is one or more of soybean meal, cotton meal, rice bran meal, corn germ meal, or the expanded products of the above-mentioned cake;

The by-products of food processing are one or more of bran, rice bran, corn gluten powder, and DDGS;

The grains are corn, broken rice, wheat, oats, barley, or one or more of the puffed products of the above grains;

The molasses syrup is one or more of glucose syrup/powder, sucrose molasses and beet molasses.

Further, the preparation conditions of silage in step 1. are as follows:

The water content of the fresh pasture or root and tuber is 30%-90%, the fineness is 0.01-100cm, the inoculation amount of lactic acid bacteria seed solution is 0.01%-20%, the compound enzyme addition is 0.0001%-2%, and the compound enzyme is composed of the following weight ratio: Enzyme composition: the weight ratio of cellulase, xylanase, pectinase and mannanase is 100:1～500:1～500:1～300;

The above percentages are percentages by mass.

Preferably, the preparation conditions of silage in step 1. are as follows:

The water content of the fresh pasture or root and tuber is 65%-75%, the fineness is 1-2cm, the inoculation amount of lactic acid bacteria seed solution is 1%-3%, the compound enzyme addition is 0.1%-0.5%, and the compound enzyme is composed of the following weight ratio: Enzyme composition: the weight ratio of cellulase, xylanase, pectinase and mannanase is 100:20-30:20-30:10-20.

Further, the preparation conditions of the fermented concentrate wet base in step ② are as follows:

The weight ratio of clean water, cake, food processing by-products, grains, and molasses syrup is 100:1~1000:0~300:0~100:0~100;

The inoculation amount of lactic acid bacteria seed solution is 1% to 30%;

The amount of compound enzyme added is 0.001% to 5%;

Fermentation temperature: 6～60℃;

Initial fermentation pH: natural;

Fermentation type: anaerobic fermentation;

Medium water content: 15% to 90%;

Fermentation time: 6-480 hours;

The above percentages are percentages by mass.

Preferably, the preparation conditions of the fermented concentrate wet base in step 2. are as follows:

The weight ratio of clean water, cake, food processing by-products, grains, and molasses syrup is 100:50-60:20-30:10-20:5-10;

The inoculum amount of lactic acid bacteria seed solution is 4% to 8%;

The amount of compound enzyme added is 0.12% to 0.6%;

Fermentation temperature: 24～28℃;

Initial fermentation pH: natural;

Fermentation type: anaerobic fermentation;

Medium water content: 40% to 60%;

Fermentation time: 48 to 72 hours.

Further, the weight ratio of the silage, fermented concentrate wet basis, aerobic fermentation seed liquid and stone powder is 100:1-1000:1-100:0.1-10;

The aerobic fermentation seed liquid is composed of a yeast seed liquid, a Bacillus subtilis liquid and a Bacillus licheniformis liquid, wherein the live bacterial spore ratio of the yeast seed liquid, the Bacillus subtilis liquid and the Bacillus licheniformis liquid is 100 : 1～1000: 1～500;

The fermentation condition of step 4. is as follows:

Fermentation temperature: 26～32℃;

Initial fermentation pH: natural;

Fermentation type: aerobic fermentation;

Medium water content: 20-80%;

Fermentation time: 3 to 360 hours;

The above percentages are percentages by mass.

Preferably, the weight ratio of the silage, fermented concentrate wet basis, aerobic fermentation seed liquid and stone powder is 100:30~100:6~12:0.005~1

The aerobic fermentation seed liquid is composed of a yeast seed liquid, a Bacillus subtilis liquid and a Bacillus licheniformis liquid, wherein the live bacterial spore ratio of the yeast strain, Bacillus subtilis and Bacillus licheniformis is 100:1～ 1000: 1~500;

The fermentation condition of step 4. is as follows:

Fermentation temperature: 26～32℃;

Initial fermentation pH: natural;

Fermentation type: aerobic fermentation;

Medium water content: 50% to 60%;

Fermentation time: 24 to 48 hours.

The beneficial effect of the present invention is that: in the fermentation process of the present invention, the fresh pasture or root and tuber not only does not produce nitrite, but also does not rot. Simultaneously, the zymolytic concentrate silage prepared by the present invention is rich in fermentable cellulose lactic acid, calcium lactate, small peptides, amino acids, vitamins and probiotics, has the characteristics of high nutrition, easy digestion, good palatability, etc., and can significantly improve the Pig milk production, significantly increased feed intake, significantly reduced sow constipation.

Detailed ways

The present invention will be described in more detail below in conjunction with examples.

Example 1

A method for preparing fermented concentrate silage special for sows, the steps are as follows:

①. Preparation of silage:

Dry the fresh alfalfa to a moisture content of 40-50%. 500kg of fresh alfalfa with a water content of 40-50% was chopped and broken to 1cm by a crushing beater to obtain 500kg of fresh alfalfa powder. 100kg of carrots with a moisture content of 80-85% are crushed and beaten to obtain 100kg of carrot pulp.

500kg of fresh alfalfa powder and 100kg of carrot pulp are mixed to make 600kg of silage raw materials with a moisture content of about 75%. Insert 10kg of lactic acid bacteria seed liquid and mix in 60g of compound enzyme (40g of cellulase, 8g of xylanase, 8g of pectinase and 4g of mannanase in the compound enzyme), fill and compact in the silo, and naturally enzymatically and After 15 days of anaerobic fermentation, 595 kg of silage was obtained.

②. Preparation of fermented concentrate wet base:

After mixing 80kg clean water, 85kg soybean meal, 80kg corn gluten powder and 20kg molasses syrup, insert 25kg lactic acid bacteria seed liquid and mix into 80g compound enzyme (50g of cellulase, 12g of xylanase, 12g of pectinase and Mannanase 6g), carry out anaerobic fermentation for 48-60 hours under the conditions of 25-26°C and natural fermentation pH, and obtain 278-282kg of fermented concentrate wet base;

③. Preparation of concentrate silage fermentation substrate:

After fully mixing 595kg of silage, 278-282kg of fermented concentrate wet base and 2-3kg of stone powder, 873-877kg of concentrate silage fermentation substrate is prepared.

④. Preparation of fermented concentrate silage for sows:

In 873-877kg of concentrate silage fermentation substrate, add 50kg of aerobic bacteria seed liquid to ferment for 48 hours, and then make 915-920kg of fermented concentrate silage wet base specially for sows, or dry it to make 425-430kg Special fermented concentrate silage dry basis for sows.

Example 2

A method for preparing fermented concentrate silage special for sows, the steps are as follows:

①. Preparation of silage:

The fresh silage corn stalks are air-dried to a moisture content of 65-75%. Using a crushing beater, 1000 kg of fresh silage corn stalks with a water content of 65-75% are chopped and crushed to 1 cm to obtain 1000 kg of fresh silage corn stalk powder.

Inject 10kg of lactic acid bacteria seed solution and mix 30g of compound enzyme (20g of cellulase, 4g of xylanase, 4g of pectinase and 2g of mannanase in compound enzyme) into 1000kg of fresh silage corn stalk powder, pack and compact in In the silo, after 15 days of natural enzymatic hydrolysis and anaerobic fermentation, 995kg of silage was obtained.

②. Preparation of fermented concentrate wet base:

After mixing 100kg of clean water, 100kg of soybean meal, 50kg of corn gluten powder, 50kg of rice bran, and 20kg of molasses syrup, insert 30kg of lactic acid bacteria seed liquid and mix in 80g of compound enzyme (50g of cellulase, 12g of xylanase, pectin in the compound enzyme) Enzyme 12g and mannanase 6g), under the condition of 25-26 ℃, fermentation pH natural, carry out anaerobic fermentation 48-60 hours, make 340-345kg fermented concentrate wet basis;

③. Preparation of concentrate silage fermentation substrate:

After fully mixing 995kg of silage, 340-345kg of fermented concentrate wet base and 3-4kg of stone powder, the pH is adjusted to 6.5-7.5 to obtain 1335-1355kg of concentrate silage fermentation substrate.

④. Preparation of fermented concentrate silage for sows:

In 1335-1355kg of concentrate silage fermentation substrate, add 80kg of aerobic bacteria seed liquid to ferment for 48 hours, and then make 1405-1410kg of fermented concentrate silage wet base specially for sows, or dry it to make 550-560kg Special fermented concentrate silage dry basis for sows.

Example 3

A method for preparing fermented concentrate silage special for sows, the steps are as follows:

①. Preparation of silage:

Dry the alfalfa to a moisture content of 50-60%. Using a crushing beater, 1000 kg of fresh silage corn stalks with a moisture content of 50-60% are chopped and crushed to 1 cm to obtain 1000 kg of fresh silage corn stalk powder.

Insert 20kg of lactic acid bacteria seed solution into 1000kg of fresh silage corn stalk powder, mix with 30g of compound enzyme (20g of cellulase, 4g of xylanase, 4g of pectinase and 2g of mannanase in the compound enzyme), pack and compact In the silo, after natural enzymatic hydrolysis and anaerobic fermentation for 15 days, 1110kg of silage was obtained.

②. Preparation of fermented concentrate wet base:

After mixing 100kg of clean water, 100kg of soybean meal, 50kg of DDGS, 50kg of bran, and 20kg of molasses syrup, insert 20kg of lactic acid bacteria seed liquid and mix in 50g of compound enzyme (30g of cellulase, 8g of xylanase, and 8g of pectinase in the compound enzyme) and mannanase 4g), carry out anaerobic fermentation for 48-60 hours under the conditions of 25-26°C and natural fermentation pH, and obtain 330-335kg of fermented concentrate wet base;

③. Preparation of concentrate silage fermentation substrate:

After fully mixing 1110kg of silage, 330-335kg of fermented concentrate wet base and 2-3kg of stone powder, the pH is adjusted to 6.5-7.5 to obtain 1440-1445kg of concentrate silage fermentation substrate.

④. Preparation of fermented concentrate silage for sows:

Into 1440-1445kg concentrate silage fermentation substrate, add 80kg aerobic bacteria seed liquid to ferment for 48 hours, and then make 1515-1520kg fermented concentrate silage wet base specially for sows, or dry it to make 545-550kg Special fermented concentrate silage dry basis for sows.

Example 4

A method for preparing fermented concentrate silage special for sows, the steps are as follows:

①. Preparation of silage:

Dry the carrots to a moisture content of 70-80%. 1000 kg of carrots with a moisture content of 70-80% are crushed and beaten by a crushing and beating machine to obtain 1000 kg of fresh carrot pulp.

Inject 30kg of lactic acid bacteria seed solution into 1000kg of fresh carrot pulp, mix with 60g of compound enzyme (40g of cellulase, 8g of xylanase, 8g of pectinase and 4g of mannanase in the compound enzyme), pack and compact in the silo 1130kg of silage was prepared after natural enzymolysis and anaerobic fermentation for 15 days.

②. Preparation of fermented concentrate wet base:

After mixing 500kg clean water, 500kg soybean meal, 300kgDDGS, 200kg bran, and 100kg molasses, insert 100kg lactic acid bacteria seed liquid and mix 300g compound enzyme (200g cellulase, 40g xylanase, 40g pectinase and Mannanase 20g), under the conditions of 25-26°C and natural fermentation pH, carry out anaerobic fermentation for 48-60 hours, and obtain 1680-1685kg of wet base of fermented concentrate;

③. Preparation of concentrate silage fermentation substrate:

After fully mixing 1130kg of silage, 1680-1685kg of fermented concentrate wet base and 5-6kg of stone powder, the pH is adjusted to 6.5-7.5 to obtain 2850-2860kg of concentrate silage fermentation substrate.

④. Preparation of fermented concentrate silage for sows:

Into 2850-2860kg of concentrate silage fermentation substrate, add 200kg of aerobic bacteria seed liquid to ferment for 48 hours, and then make 2980-3010kg of fermented concentrate silage wet base specially for sows, or dry it to make 1145-1150kg Special fermented concentrate silage dry basis for sows.

Example 5

A method for preparing fermented concentrate silage special for sows, the steps are as follows:

Dry the fresh grain amaranth until the water content is 40-45%. Utilize crushing beater, 1000kg moisture content is that the fresh alfalfa of 40-50% is cut short and broken to 1cm, makes 1000kg fresh fresh grain amaranth powder. 500kg of sweet potatoes with a moisture content of 80-85% are crushed and beaten to obtain 500kg of sweet potato pulp.

1000kg of fresh alfalfa powder and 500kg of carrot pulp are mixed to make 1500kg of silage raw materials with a moisture content of about 75%. Insert 50kg of lactic acid bacteria seed liquid and mix in 60g of compound enzyme (40g of cellulase, 8g of xylanase, 8g of pectinase and 4g of mannanase in the compound enzyme), fill and compact in the silo, and naturally enzymatically and After 15 days of anaerobic fermentation, 1590 kg of silage was obtained.

②. Preparation of fermented concentrate wet base:

After mixing 400kg of clean water, 500kg of soybean meal, 300kg of corn gluten powder, 200kg of liquor distiller's grains, and 150kg of molasses, insert 150kg of lactic acid bacteria seed liquid and mix in 300g of compound enzyme (200g of cellulase, 40g of xylanase, and pectin in the compound enzyme) Enzyme 40g and mannanase 20g), under the condition of 25-26 ℃, fermentation pH natural, carry out anaerobic fermentation 48-60 hours, make 1690-1700kg fermented concentrate wet basis;

③. Preparation of concentrate silage fermentation substrate:

After fully mixing 1590kg of silage, 1690-1700kg of fermented concentrate wet base and 5-6kg of stone powder, the pH is adjusted to 6.5-7.5 to obtain 3265-3270kg of concentrate silage fermentation substrate.

④. Preparation of fermented concentrate silage for sows:

Into 3265-3270kg concentrate silage fermentation substrate, insert 150kg compound aerobic bacteria seed solution and ferment for 48 hours, and then make 3380-3390kg fermented concentrate silage wet base specially for sows, or dry 1650- 1680kg special fermented concentrate silage dry basis for sows.

1. See Table 1 for the contents of main nutrients in the special fermented concentrate silage for sows prepared in Example 1.

Table 1, content of nutrients in the fermented concentrate silage product specially prepared for sows in Example 1

project index project index project index color tan L-lactic acid/% ≥5.0 Fermentable fiber/% ≥1.5 Moisture/% ≤12.0 L-Calcium Lactate/% ≥0.5 Total number of probiotics cfu/g ≥0.03×10⁶ crude protein/% ≥18.0 Cellulose/% ≥6.0 water soluble substances ≥20.0

Two, the effect of feeding lactating sows with the fermented concentrate silage special for sows prepared in Example 1.

In order to investigate the feeding effect of the special zymolytic concentrate silage for sows prepared in Example 1 on lactating sows, 120 growing lactating sows with similar blood relationship, parity, body weight and farrowing date were selected. The number of litters is 10.5, and the average weight of sows is 220 kg. They are randomly divided into 2 groups (control group and test group). Feed 8% fermented concentrate silage specially for sows in the experimental group feed (here refers to adding 8% mass fraction of fermented concentrate silage specially for sows in the basal ration), and the control group does not add The basal ration of fermented concentrate silage for sows. The basal diets of the control group and the experimental group were the same.

Table 2. Basal diet composition and nutritional level

The test was carried out for 35 days (from calving to estrus again), and the pigs were fed according to the conventional feeding method of the pig farm. Wet mixtures were fed ad libitum and water was available ad libitum. At the beginning and end of the experiment, the individuals in each group were weighed, the body loss was calculated, and the feed intake and weight gain of piglets were counted.

It can be seen from Table 3 that after adding 8% special fermented concentrate silage for sows in the basic diet, the feed intake of sows increased from 5.0kg/day to 5.5kg/day for 8-28 days, an increase of 10.0%. , and no significant loss of body weight was observed in sows.

It can be seen from Table 4 that after adding 8% special fermented concentrate silage for sows in the basic diet, the average body weight of suckling piglets at weaning was 10.45% higher than that of the control group; the weight loss of sows was reduced by 33.3%; days reduced to 6.0 days, shortened by 0.5 days.

Table 3, the effect of the special fermented concentrate silage for sows prepared in Example 1 on feed intake and body weight of sows

Table 4, the influence of the special zymolysis concentrate silage for sows prepared in Example 1 on the performance of piglets

group Average starting weight(kg) Weight at the end of weaning (kg) weight gain (kg) More weight gain (kg) Incidence rate (%) control group 1.35 6.7 5.35 - 8.3 test group 1.3 7.4 6.1 0.75 1.2

3. The effect of feeding hybrid piglets the special fermented concentrate silage for sows prepared in Example 1.

In order to investigate the feeding effect of the special zymolytic concentrate silage for sows prepared in Example 1 on hybrid piglets, select 168 long-term hybrid piglets (59 and 60 days) with similar body weight (body weight 18.9 ± 0.85kg). age piglets), were randomly divided into test group and control group, 84 test groups (44 sows, 40 boars), 84 control groups (38 sows, 46 boars). The weights of piglets in the two groups were similar (the significance analysis was not significant), and each group had 7 replicates, each with 12 piglets.

The basal diets of piglets in the experimental group and the control group were the same, both being powder. The control group did not add special fermented concentrate silage for sows, and the test group added 6% special fermented concentrate silage for sows. The diet was mixed evenly, and the coefficient of variation was determined to be less than 8%. The diet configuration is based on the G national standard GB/T5915 piglet and growing-finishing pig compound feed standard, and its composition and nutritional components are shown in Table 5. Its corn, soybean meal, wheat bran, imported fish meal and other feed materials have been tested to meet the quality requirements of the national standard (GB13078-2001).

Table 5. Basic diet composition and nutritional level of experimental piglets

Raw material composition content/% nutrient content corn 61.00 Digestible energy DE 12.35MJ kg^-1 soybean meal 25.00 dry matter DM 87.74% (measured) Wheat bran 6.00 crude fat EE 3.52% imported fish meal 3.00 crude protein CP 17.02% Grease powder 1.00 crude fiber CF 4.22% Premix 4.00

Note: During the test period, the premix contains stone powder, calcium hydrogen phosphate, salt, 20% lysine, vitamins and trace elements.

The test officially started on January 7, 2016, and officially ended on March 1, 2016, with a total of 54 days. The test results are as follows:

It can be seen from Table 6 that after adding 6% special fermented concentrate silage for sows in the diet, the average final weight of 42 piglets in the test group was 38.06 kg, and the average final weight of the control group was 35.71 kg, which was more than that of the control group. 2.35kg, an increase of 6.58% (P<0.05); the average weight gain of the test group was 19.24kg, and the average weight gain of the control group was 16.66kg, and the test group was 2.58kg more than the control group, an increase of 15.49% (P<0.01); The average daily weight gain of the group was 356.30g, the average daily weight gain of the control group was 308.52g, and the test group was 47.78g more than the control group, an increase of 15.49% (P<0.01).

Table 6, the influence of the special fermented concentrate silage for sows prepared in Example 1 on the daily gain of piglets

Note: ** indicates significant difference within a column (P<0.01), * indicates significant difference within a column (P<0.05). Table 2 is the same.

It can be seen from Table 7 that after adding 6% special zymolytic concentrate silage for sows in the diet, the feed intake per head of the test group was 45.21 kg, and the feed intake per head of the control group was 40.32 kg, which was more in the test group than in the control group. 40.89kg, the feed intake increased by 12.13% (P<0.05); the feed conversion rate of the test group was 2.35, 0.07 less than the control group 2.42, and the test group was 2.89% lower than the control group (P>0.05).

Table 7, the influence of the fermented concentrate silage specially prepared for sows on feed intake and feed conversion ratio prepared in Example 1

group Head count Weight gain/head/kg Feed intake/head/kg feed conversion ratio Feed cost/yuan control group 84 16.66 40.32±1.23 2.42±0.49 60.48 test group 84 19.24 45.21±2.35* 2.35±0.83 67.82

The above content is only an example and description of the present invention. Those skilled in the art can make various modifications or supplements to the described specific embodiments or use similar methods to replace them, as long as they do not deviate from the concept of the invention or go beyond The scope defined in the claims shall belong to the protection scope of the present invention.

Claims (8)

1. A preparation method for special zymolysis concentrate silage for sows, characterized in that the steps are as follows: ①. Preparation of silage: Cut fresh grass and/or root tubers into short pieces, beat them, mix them with lactic acid bacteria seed solution and compound enzyme, fill and compact them in silo or tower, and make silage after natural enzymatic hydrolysis and anaerobic fermentation; ②. Preparation of fermented concentrate wet base: Mix one or more of cakes, by-products of food processing other than cakes, and grains with clean water and molasses syrup, then mix them with lactic acid bacteria seed solution and compound enzymes, and natural enzymatic hydrolysis and anaerobic fermentation to obtain wet basis of fermented concentrate; ③. Preparation of concentrate silage fermentation substrate: After mixing silage, fermented concentrate wet base and stone powder, the concentrate silage fermentation substrate is prepared; ④. Preparation of fermented concentrate silage for sows: Add the aerobic fermentation strain solution to the concentrate silage fermentation substrate, and after natural aerobic fermentation, the special zymolytic concentrate silage wet base for sows is obtained, or the special zymolytic concentrate silage for sows is obtained after drying Feed dry basis. 2. the preparation method of a kind of special zymolysis concentrate silage for sows according to claim 1, is characterized in that: The fresh pasture and/or root tuber is one or more of the fresh pasture and/or root tuber feed in the feed material catalog of the Ministry of Agriculture, which is silage corn, alfalfa grass, clover, water hyacinth, water duckweed, water peanut , one or more of grain amaranth, carrot, cassava, sweet potato, pumpkin, potato; The cake is one or more of the cake feed in the feed raw material catalog of the Ministry of Agriculture, including soybean meal, rapeseed meal, cotton meal, peanut meal, linseed meal, sunflower seed meal, rice bran meal, corn germ meal, Or one or more of the above-mentioned puffed cake products; The by-products of food processing other than cakes are one or more of the by-products of food processing other than cakes in the feed raw material catalog of the Ministry of Agriculture, such as bran, rice bran, wheat gluten, corn gluten powder, rice protein One or more of powder, DDGS, liquor distillers grains, beer distillers grains; The grain is one or more of the grain products listed in the feed material catalog of the Ministry of Agriculture, corn, rice, broken rice, wheat, flour, oats, barley, sorghum, or one or more of the puffed products of the above grains. various; The molasses syrup is one or more of the molasses syrups in the feed material catalog of the Ministry of Agriculture, including sucrose syrup/powder, glucose syrup/powder, fructose syrup/powder, maltodextrin pulp/powder, maltose syrup/powder, sucrose One or more of molasses, beet molasses, and soybean molasses. 3. the preparation method of a kind of special zymolysis concentrate silage for sows according to claim 2, is characterized in that: The fresh pasture and/or root and tuber are one or more of silage corn, alfalfa grass, sweet potato, carrot, and pumpkin; The cake is one or more of soybean meal, cotton meal, rice bran meal, corn germ meal, or the expanded products of the above-mentioned cake; The by-products of food processing other than cakes are one or more of bran, rice bran, corn gluten meal, and DDGS; The grains are corn, broken rice, wheat, oats, barley, or one or more of the puffed products of the above grains; The molasses syrup is one or more of glucose syrup/powder, sucrose molasses and beet molasses. 4. the preparation method of a kind of sow special-purpose zymolysis concentrate silage according to claim 1, is characterized in that: the preparation condition of step ① middle silage is as follows: The water content of the fresh pasture or root and tuber is 30%-90%, the fineness is 0.01-100cm, the inoculation amount of lactic acid bacteria seed solution is 0.01%-20%, the compound enzyme addition is 0.0001%-2%, and the compound enzyme is composed of the following weight ratio: Enzyme composition: the weight ratio of cellulase, xylanase, pectinase and mannanase is 100:1～500:1～500:1～300; The above percentages are percentages by mass. 5. the preparation method of a kind of sow special-purpose zymolysis concentrate silage according to claim 4, is characterized in that: the preparation condition of step 1. middle silage is as follows: The water content of the fresh pasture or root and tuber is 65%-75%, the fineness is 1-2cm, the inoculation amount of lactic acid bacteria seed solution is 1%-3%, the compound enzyme addition is 0.1%-0.5%, and the compound enzyme is composed of the following weight ratio: Enzyme composition: the weight ratio of cellulase, xylanase, pectinase and mannanase is 100:20-30:20-30:10-20. 6. the preparation method of a kind of sow special fermented concentrate silage according to claim 1, is characterized in that, the preparation condition of fermented concentrate wet base in step 2. is as follows: The weight ratio of clean water, cake, food processing by-products except cake, grain, and molasses syrup is 100:50-60:20-30:10-20:5-10; The inoculum amount of lactic acid bacteria seed solution is 4% to 8%; The amount of compound enzyme added is 0.12% to 0.6%; Fermentation temperature: 24～28℃; Initial fermentation pH: natural; Fermentation type: anaerobic fermentation; Medium water content: 40% to 60%; Fermentation time: 48 to 72 hours. 7. the preparation method of a kind of sow special-purpose zymolysis concentrate silage according to claim 1, is characterized in that, The weight ratio of the silage, fermented concentrate wet basis, aerobic fermentation seed liquid and stone powder is 100:1-1000:1-100:0.1-10; The aerobic fermentation seed liquid is composed of a yeast seed liquid, a Bacillus subtilis liquid and a Bacillus licheniformis liquid, wherein the live bacterial spore ratio of the yeast seed liquid, the Bacillus subtilis liquid and the Bacillus licheniformis liquid is 100 : 1～1000: 1～500; The fermentation condition of step 4. is as follows: Fermentation temperature: 26～32℃; Initial fermentation pH: natural; Fermentation type: aerobic fermentation; Medium water content: 20-80%; Fermentation time: 3 to 360 hours; The above percentages are percentages by mass. 8. the preparation method of a kind of sow special-purpose zymolysis concentrate silage according to claim 7, is characterized in that, The weight ratio of the silage, fermented concentrate wet base, aerobic fermentation seed liquid and stone powder is 100:30-100:6-12:0.1-1; The aerobic fermentation seed liquid is composed of a yeast seed liquid, a Bacillus subtilis liquid and a Bacillus licheniformis liquid, wherein the live bacterial spore ratio of the yeast strain, Bacillus subtilis and Bacillus licheniformis is 100:1～ 1000: 1~500; The fermentation condition of step 4. is as follows: Fermentation temperature: 26～32℃; Initial fermentation pH: natural; Fermentation type: aerobic fermentation; Medium water content: 50% to 60%; Fermentation time: 24 to 48 hours.