CN106069727A - A kind of artificial pollination method for pear tree - Google Patents
A kind of artificial pollination method for pear tree Download PDFInfo
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- CN106069727A CN106069727A CN201610497946.2A CN201610497946A CN106069727A CN 106069727 A CN106069727 A CN 106069727A CN 201610497946 A CN201610497946 A CN 201610497946A CN 106069727 A CN106069727 A CN 106069727A
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- 230000010152 pollination Effects 0.000 title claims abstract description 51
- 235000014443 Pyrus communis Nutrition 0.000 title claims abstract description 29
- 240000001987 Pyrus communis Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 239000000575 pesticide Substances 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 9
- 239000004745 nonwoven fabric Substances 0.000 claims description 9
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims description 3
- 235000019743 Choline chloride Nutrition 0.000 claims description 3
- 244000303965 Cyamopsis psoralioides Species 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 235000019270 ammonium chloride Nutrition 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 229960003178 choline chloride Drugs 0.000 claims description 3
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 3
- 238000006297 dehydration reaction Methods 0.000 claims description 3
- RCVCNFJNZUYOGY-UHFFFAOYSA-N dodecyl hydroxy propyl phosphate Chemical compound P(=O)(OCCCCCCCCCCCC)(OO)OCCC RCVCNFJNZUYOGY-UHFFFAOYSA-N 0.000 claims description 3
- 150000002148 esters Chemical class 0.000 claims description 3
- 235000001727 glucose Nutrition 0.000 claims description 3
- 150000002304 glucoses Chemical class 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 210000004681 ovum Anatomy 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 244000088401 Pyrus pyrifolia Species 0.000 claims 3
- 230000000694 effects Effects 0.000 abstract description 7
- 235000013399 edible fruits Nutrition 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 3
- 239000002917 insecticide Substances 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 3
- 241000220324 Pyrus Species 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 238000005507 spraying Methods 0.000 abstract description 2
- 244000025272 Persea americana Species 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 241000256836 Apis Species 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 241000256844 Apis mellifera Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 230000035943 smell Effects 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of artificial pollination method for pear tree, comprise the steps: that prepared by (1) pollen, prepared by (2) petal extracting solution, prepared by (3) sepal extracting solution, prepared by (4) pollen pollination liquid, (5) artificial pollination.The pollen pollination liquid effect duration at normal temperatures of uniqueness of the present invention preparation is two days, during this, the vigor of pollen is without substantially reducing, improve the problem needing in existing mode repeatedly to prepare pollination liquid, reduce labor intensity, the pollination activity intensity of the insecticides such as Apis can be strengthened again simultaneously, compensate for ensure, when spraying, the problem that flower is all capped, the percentage of fertile fruit of final pear flower is up to more than 98%, greatly improve planting benefit, additionally, the inventive method is simple, pollen consumption is few, cost is relatively low, has good promotional value.
Description
Technical field
The invention belongs to field of planting, be specifically related to a kind of artificial pollination method for pear tree.
Background technology
Pear is in gametophytic incompatibility fruit tree, and most kind self-pollinations can not be solid, miscellaneous by remote edge
Handing over and breed, use and configure suitable pollinizers and cultivar and mutually pollinate to improve solid on producing, pear pollen is main
Mediate by mild wind and honeybee worm and to teach.Frequently with artificial supplementary pollination technology to improve fruit-setting rate, really in pears production process
Protect yield and quality.
Existing artificial pollination mode mostly is artificial point and awards method, is i.e. directly spread upon by pollen female by brush pen or banister brush
On the style of flower, this mode also exists that cost of labor is high, take time and effort, expend the shortcomings such as pollen is many, and then people explore trial
Liquid pollination mode, will be added in liquid flux by pollen, is then sprayed on the stigma of female flower, has pollination speed fast,
Save artificial feature, and moisture and the nutrient of stigma can be increased, extend the stigma Pollination Fertilization time, but liquid pollination technology is still
Immature, however it remains nutritional solution suspending power poor, pollen dispersion is uneven, the problem of easy swelling death in nutritional solution,
And the pollen solution prepared need to make to be finished at short notice, usually within 5h, not so pollen loses vigor again without exploitation value
Value, this also increases the fussy degree of preparation during pollination, the most inevitably causes pollen a certain degree of simultaneously
Waste.Although additionally, liquid pollination mode is the most convenient, but full-bloom stage flower is the most intensive, during sprinkling, cannot ensure that flower is equal
Capped, the situation that part flower still can be caused to pollinate, it is unfavorable for the raising of pollination rate.
Before pear tree enters the florescence, it usually needs carry out flower thinning operation, reduce unnecessary nutrition leak, to increase fruit
Quality, can produce substantial amounts of useless flower during flower thinning, when pear tree grows naturally also can withered a lot of flowers, current fresh rare to this
The method that class flower fully recycles.
Summary of the invention
In order to solve the problems referred to above, the invention provides a kind of artificial pollination method for pear tree, have method simple,
The feature that pollination rate is high.
The present invention is achieved through the following technical solutions:
A kind of artificial pollination method for pear tree, comprises the steps:
(1) prepared by pollen:
A. pear flower entered after florescence, in the morning before 8.30, selected the male flower that will open, standby under plucking at anthocaulus,
If excess moisture on male flower, the most first put in drier standby after dehydration；
B. being placed on by male flower in the mesh screen that aperture is 4mm, spread non-woven fabrics below mesh screen, both hands are rubbed gently, make flower pesticide and flower
Lobe and filigree are peeled off, then shake mesh screen, are sieved to flower pesticide be layered on the non-woven fabrics below mesh screen, regather the flower pesticide on non-woven fabrics
Pour into and the plastics mesh screen that aperture is 3 mm is carried out sieve for the second time, reject the impurity such as petal and filigree, obtain clean flower pesticide
Standby；
C. gained flower pesticide is put under conditions of temperature is 18 ~ 23 DEG C and toasts, treat that flower pesticide pops, after the pollen of yellow flows out, stop
Only baking, crosses 200 mesh sieves by flower pesticide and pollen mixture, obtains pear flower pollen standby；
(2) prepared by petal extracting solution:
A. pear flower petal is separated from flower, then put it under conditions of temperature is 38 ~ 42 DEG C, dry to water content
It is 20 ~ 25% standby；
B. the pear flower petal after drying is put into after being ground into rotten shape thing in pulverizer, further takes out and puts into its gross mass 3 times
Volumetric concentration is in the ethanol solution of 75%, extraction 10h after, filter petal extracting solution is standby；
(3) prepared by sepal extracting solution:
A. pear flower sepal is separated from flower, then put it under conditions of temperature is 40 ~ 43 DEG C, dry to water content
It is 15 ~ 20% standby；
B. the pear flower sepal after drying is put into after being ground into rotten shape thing in pulverizer, further takes out and puts into its gross mass 3 times
Volumetric concentration is in the ethanol solution of 85%, extraction 10h after, filter sepal extracting solution is standby；
(4) prepared by pollen pollination liquid:
Weigh following material the most by weight: 20 parts of sucrose, 10 parts of glucoses, 4 parts of boric acid, 7 parts of guar gums, 2 parts of Semen sojae atricolor ovum phosphorus
Fat, 1.5 parts of dodecyl Hydroxypropyl phosphate ester glycine betaines, 1 part of potassium nitrate, 1 part of ammonium chloride, 0.5 part of choline chloride；
B. take the deionized water of mentioned component gross mass 400 ~ 500 times, be heated to 40 ~ 42 DEG C, again that mentioned component is the most common
Put in deionized water, obtain base fluid after being stirred continuously mix homogeneously standby；
C. by the pollen of step (1) gained, the petal extracting solution of step (2) gained, the sepal extracting solution of step (3) gained and
Base fluid is by weight i.e. obtaining pollen pollination liquid after 0.1:7:3:100 mix homogeneously；
(5) artificial pollination: when pear flower enters full-bloom stage, by pollen pollination liquid atomizing pollination device spray made for step (4)
Being sprinkled upon female taking, the time of sprinkling is selected in morning 7.00 ~ 9.00 or afternoon 2.00 ~ 4.00.
There is advantages that
(1) the useless flower produced during pear flower is grown by the present invention is made full use of, and petal and sepal is therefrom carried out
Separate, prepare petal extracting solution and sepal extracting solution respectively, add in pollen pollination liquid, on the one hand can improve the work of pollen
Power, with the problem that the components matching such as sucrose improves the easy swelling death of pollen, extends the effective life of pollen pollination liquid, another
Containing multiple natural chemical substance in aspect, petal extracting solution and sepal extracting solution, special abnormal smells from the patient can be given out, again can be spy
Visible special fluorescence under standing wave length, this type of fluorescence can attract and guide the insecticides such as Apis to come to suck nectar, and then auxiliary passes
Broadcast pollen, improve pollination success rate further.
(2) the pollen pollination liquid effect duration at normal temperatures of uniqueness of the present invention preparation is two days, pollen during this
Vigor, without substantially reducing, improves the problem needing repeatedly to prepare pollination liquid in existing mode, reduces labor intensity, again can simultaneously
Strengthen the pollination activity intensity of the insecticides such as Apis, compensate for ensure the problem that flower is all capped, final pear flower when spraying
Percentage of fertile fruit up to more than 98%, greatly improve planting benefit, additionally, the inventive method is simple, pollen consumption is few, and cost is relatively
Low, there is good promotional value.
Detailed description of the invention
Embodiment 1
A kind of artificial pollination method for pear tree, comprises the steps:
(1) prepared by pollen:
A. pear flower entered after florescence, in the morning before 8.30, selected the male flower that will open, standby under plucking at anthocaulus,
If excess moisture on male flower, the most first put in drier standby after dehydration；
B. being placed on by male flower in the mesh screen that aperture is 4mm, spread non-woven fabrics below mesh screen, both hands are rubbed gently, make flower pesticide and flower
Lobe and filigree are peeled off, then shake mesh screen, are sieved to flower pesticide be layered on the non-woven fabrics below mesh screen, regather the flower pesticide on non-woven fabrics
Pour into and the plastics mesh screen that aperture is 3 mm is carried out sieve for the second time, reject the impurity such as petal and filigree, obtain clean flower pesticide
Standby；
C. gained flower pesticide is put under conditions of temperature is 18 ~ 23 DEG C and toasts, treat that flower pesticide pops, after the pollen of yellow flows out, stop
Only baking, crosses 200 mesh sieves by flower pesticide and pollen mixture, obtains pear flower pollen standby；
(2) prepared by petal extracting solution:
A. pear flower petal is separated from flower, then put it under conditions of temperature is 38 ~ 42 DEG C, dry to water content
It is 20 ~ 25% standby；
B. the pear flower petal after drying is put into after being ground into rotten shape thing in pulverizer, further takes out and puts into its gross mass 3 times
Volumetric concentration is in the ethanol solution of 75%, extraction 10h after, filter petal extracting solution is standby；
(3) prepared by sepal extracting solution:
A. pear flower sepal is separated from flower, then put it under conditions of temperature is 40 ~ 43 DEG C, dry to water content
It is 15 ~ 20% standby；
B. the pear flower sepal after drying is put into after being ground into rotten shape thing in pulverizer, further takes out and puts into its gross mass 3 times
Volumetric concentration is in the ethanol solution of 85%, extraction 10h after, filter sepal extracting solution is standby；
(4) prepared by pollen pollination liquid:
Weigh following material the most by weight: 20 parts of sucrose, 10 parts of glucoses, 4 parts of boric acid, 7 parts of guar gums, 2 parts of Semen sojae atricolor ovum phosphorus
Fat, 1.5 parts of dodecyl Hydroxypropyl phosphate ester glycine betaines, 1 part of potassium nitrate, 1 part of ammonium chloride, 0.5 part of choline chloride；
B. take the deionized water of mentioned component gross mass 400 ~ 500 times, be heated to 40 ~ 42 DEG C, again that mentioned component is the most common
Put in deionized water, obtain base fluid after being stirred continuously mix homogeneously standby；
C. by the pollen of step (1) gained, the petal extracting solution of step (2) gained, the sepal extracting solution of step (3) gained and
Base fluid is by weight i.e. obtaining pollen pollination liquid after 0.1:7:3:100 mix homogeneously；
(5) artificial pollination: when pear flower enters full-bloom stage, by pollen pollination liquid atomizing pollination device spray made for step (4)
Being sprinkled upon female taking, the time of sprinkling is selected in morning 7.00 ~ 9.00 or afternoon 2.00 ~ 4.00.
Comparative example 1
This comparative example 1 compared with Example 1, does not contains petal extracting solution and sepal extracting solution in made pollen pollination liquid,
Method step in addition is the most identical.
Matched group 1
Existing common pear flower liquid pollination method.
Matched group 2
Spontaneous pollination.
For contrasting effect of the present invention, by above-mentioned four kinds of modes, pear flower being carried out pollination operation respectively, following table is the most right
Than data:
| Percentage of fertile fruit (%) | Pure pollen amount (g) needed for average every mu | Average every mu of pollination time (h) | |
| Embodiment 1 | 98.6 | 8.2 | 0.4 |
| Comparative example 1 | 93.7 | 10.5 | 0.6 |
| Matched group 1 | 89.2 | 14.6 | 1.2 |
| Matched group 2 | 70.3 | / | / |
As seen from the above table, the inventive method compared with the conventional method, is using less pollen amount, is putting into relatively low artificial pollination
Time cost, can obtain the percentage of fertile fruit effect of up to more than 98%, and be not added with petal extracting solution and the contrast of sepal extracting solution
Embodiment 1, the using effect in different factors all has decline, and made pollen pollination liquid effective life was less than 1 day.
Claims (1)
1. the artificial pollination method for pear tree, it is characterised in that comprise the steps:
(1) prepared by pollen:
A. pear flower entered after florescence, in the morning before 8.30, selected the male flower that will open, standby under plucking at anthocaulus,
If excess moisture on male flower, the most first put in drier standby after dehydration；
B. being placed on by male flower in the mesh screen that aperture is 4mm, spread non-woven fabrics below mesh screen, both hands are rubbed gently, make flower pesticide and flower
Lobe and filigree are peeled off, then shake mesh screen, are sieved to flower pesticide be layered on the non-woven fabrics below mesh screen, regather the flower pesticide on non-woven fabrics
Pour into and the plastics mesh screen that aperture is 3 mm is carried out sieve for the second time, reject the impurity such as petal and filigree, obtain clean flower pesticide
Standby；
C. gained flower pesticide is put under conditions of temperature is 18 ~ 23 DEG C and toasts, treat that flower pesticide pops, after the pollen of yellow flows out, stop
Only baking, crosses 200 mesh sieves by flower pesticide and pollen mixture, obtains pear flower pollen standby；
(2) prepared by petal extracting solution:
A. pear flower petal is separated from flower, then put it under conditions of temperature is 38 ~ 42 DEG C, dry to water content
It is 20 ~ 25% standby；
B. the pear flower petal after drying is put into after being ground into rotten shape thing in pulverizer, further takes out and puts into its gross mass 3 times
Volumetric concentration is in the ethanol solution of 75%, extraction 10h after, filter petal extracting solution is standby；
(3) prepared by sepal extracting solution:
A. pear flower sepal is separated from flower, then put it under conditions of temperature is 40 ~ 43 DEG C, dry to water content
It is 15 ~ 20% standby；
B. the pear flower sepal after drying is put into after being ground into rotten shape thing in pulverizer, further takes out and puts into its gross mass 3 times
Volumetric concentration is in the ethanol solution of 85%, extraction 10h after, filter sepal extracting solution is standby；
(4) prepared by pollen pollination liquid:
Weigh following material the most by weight: 20 parts of sucrose, 10 parts of glucoses, 4 parts of boric acid, 7 parts of guar gums, 2 parts of Semen sojae atricolor ovum phosphorus
Fat, 1.5 parts of dodecyl Hydroxypropyl phosphate ester glycine betaines, 1 part of potassium nitrate, 1 part of ammonium chloride, 0.5 part of choline chloride；
B. take the deionized water of mentioned component gross mass 400 ~ 500 times, be heated to 40 ~ 42 DEG C, again that mentioned component is the most common
Put in deionized water, obtain base fluid after being stirred continuously mix homogeneously standby；
C. by the pollen of step (1) gained, the petal extracting solution of step (2) gained, the sepal extracting solution of step (3) gained and
Base fluid is by weight i.e. obtaining pollen pollination liquid after 0.1:7:3:100 mix homogeneously；
(5) artificial pollination: when pear flower enters full-bloom stage, by pollen pollination liquid atomizing pollination device spray made for step (4)
Being sprinkled upon female taking, the time of sprinkling is selected in morning 7.00 ~ 9.00 or afternoon 2.00 ~ 4.00.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610497946.2A CN106069727A (en) | 2016-06-30 | 2016-06-30 | A kind of artificial pollination method for pear tree |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718833A (en) * | 2016-12-07 | 2017-05-31 | 延边朝鲜族自治州农业科学院（延边特产研究所） | Apple pear essence pollen pollinating method |
| CN107853168A (en) * | 2017-11-14 | 2018-03-30 | 中国林业科学研究院热带林业实验中心 | Promote the artificial pollination method of the solid production seed of manglietia glauca |
| CN109329041A (en) * | 2018-09-18 | 2019-02-15 | 吴江市黎里心怡农业科技有限公司 | A kind of Cuiguan pear pollen dilution |
| CN109329042A (en) * | 2018-09-18 | 2019-02-15 | 吴江市黎里心怡农业科技有限公司 | A kind of application of Cuiguan pear pollen dilution |
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| CN106718833B (en) * | 2016-12-07 | 2019-06-07 | 延边朝鲜族自治州农业科学院（延边特产研究所） | Apple pear essence pollen pollinating method |
| CN107853168A (en) * | 2017-11-14 | 2018-03-30 | 中国林业科学研究院热带林业实验中心 | Promote the artificial pollination method of the solid production seed of manglietia glauca |
| CN109329041A (en) * | 2018-09-18 | 2019-02-15 | 吴江市黎里心怡农业科技有限公司 | A kind of Cuiguan pear pollen dilution |
| CN109329042A (en) * | 2018-09-18 | 2019-02-15 | 吴江市黎里心怡农业科技有限公司 | A kind of application of Cuiguan pear pollen dilution |
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