CN106053364A - Kit for measuring insulin and preparation method of kit - Google Patents
Kit for measuring insulin and preparation method of kit Download PDFInfo
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- CN106053364A CN106053364A CN201610358104.9A CN201610358104A CN106053364A CN 106053364 A CN106053364 A CN 106053364A CN 201610358104 A CN201610358104 A CN 201610358104A CN 106053364 A CN106053364 A CN 106053364A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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Abstract
The invention discloses a kit for measuring insulin and a preparation method of the kit. The kit comprises two liquid components including a reagent R1 and a reagent R2 which are mutually independent, the reagent R1 comprises Tris buffer liquid, polyethylene glycol-8000, Tween-20, sodium azide, sodium EDTA and bovine serum albumin, and the reagent R2 comprises Tris buffer liquid, sodium chloride, sodium azide, bovine serum albumin and latex coated anti-insulin antibody. The preparation method includes: preparing the reagents according to component content; mixing a to-be-measured sample with the reagents R1 and R2; using a full-automatic biochemical analyzer to measure light absorbance difference value after reaction; calculating concentration of insulin in the sample according to light absorbance change value. The kit has the advantages of high measuring accuracy and the like.
Description
Technical field
The present invention relates to medical science and technological field of biochemistry, a kind of test kit measuring insulin and
Preparation method.
Background technology
Insulin is by endogenous or exogenous material by the beta Cell of islet in pancreas, such as glucose, lactose, ribose,
The stimulation of arginine, glucagon etc. and a kind of proteohormone of secreting, insulin is that unique fall is hypoglycemic in body
Hormone, simultaneously facilitates glycogen, fat, protein synthesis.Exogenous insulin is mainly used to treating diabetes.
Type 1 diabetes patient before making a definite diagnosis diabetes, major part patient's beta Cell of islet generation autoimmune destruction,
All reducing with Basal insulin secretion when causing eating, type 2 diabetes mellitus patient's islet beta cell function is abnormal makes slow progress, and often shows
For peripheral insulin resistance, therefore the detection of insulin all has for the diagnosis of diabetics and the monitoring of typing and treatment
Important meaning.
At present, the detection method of insulin has immunoelectrophoresis, immuno-precipitation, a radioimmunology etc., immunoelectrophoresis and
Immuno-precipitation complex operation, needs special instrument and the operation of specialty, requires higher to technical staff, is not suitable for clinical quick
Detection, radioimmunology contains radioelement, has the biggest pollution to environment, there is also the defect that accuracy in detection is the highest simultaneously.
Summary of the invention
The technical problem to be solved is that prior art operation is complicated, pollute environment and measure accurate in order to overcome
The defect that exactness is low, and a kind of test kit measuring insulin and preparation method thereof is provided.
The technical scheme that the present invention solves above-mentioned technical problem and provides is: the invention discloses a kind of examination measuring insulin
Agent box, including reagent R1 independent of each other and reagent R2 biliquid component, including composition and corresponding content be:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
PEG-8 000 20 ~ 60 g/L
Tween 20 10 ~ 35 g/L
Sodium azide 0.3 ~ 1.7 g/L
Sodium ethylene diamine tetracetate 1.5 ~ 6.5 mmol/L
Bovine serum albumin 10 ~ 30 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Sodium chloride 150 ~ 350 mmol/L
Sodium azide 0.3 ~ 1.7 g/L
Bovine serum albumin 10 ~ 30 g/L
Latex is coated anti-insulin antibody 1 ~ 8g/L
Its solvent is purified water.
As preferably, the invention discloses a kind of test kit measuring insulin, including reagent R1 independent of each other and reagent R2
Biliquid component, including composition and corresponding content be:
Reagent R1:
Tris buffer 100 mmol/L
PEG-8 000 40 g/L
Tween 20 20 g/L
Sodium azide 1.0 g/L
Sodium ethylene diamine tetracetate 4.0 mmol/L
Bovine serum albumin 20 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 100 mmol/L
Sodium chloride 200 mmol/L
Sodium azide 1.0 g/L
Bovine serum albumin 20 g/L
Latex is coated anti-insulin antibody 4g/L
Its solvent is purified water.
As preferably, in described reagent R2, described latex is coated the preparation method of anti-insulin antibody and is: first with 50
The polystyrene microsphere dilution that particle diameter is 80 ~ 500nm is become the matter of contained polystyrene microsphere by the MES buffer of mmol/L
Amount concentration is the solution of 1%-5%, then adds 1-ethyl-3-(3-DimethylAminopropyl) carbonization of 1.0 mg in every milliliter of solution
Diimmonium salt hydrochlorate, reacts 1 hour under conditions of 20 DEG C-37 DEG C, uses centrifuge, at 25000 rpm/ after having reacted
It is centrifuged 30 minutes under the rotating speed of min, removes supernatant, then the precipitation MES buffer of 50 mmol/L is diluted, then use super
Sound separating apparatus carries out ultrasonic disperse, re-uses centrifuge, is centrifuged 30 minutes under 25000 rpm/min rotating speeds, removes supernatant, will be heavy
Form sediment and dilute with the MES buffer of 50 mmol/L, till in solution, the mass concentration of polystyrene microsphere is 1%-3%, ultrasonic
Dispersion, then dispersion limit in limit adds isopyknic MES buffer containing 8 mg/mL anti-insulin antibody, and mix and blend, 20
React 2 hours under conditions of DEG C-37 DEG C, when the final mass concentration of polystyrene microsphere is 1% till, be subsequently adding cattle
Serum albumin so that bovine serum albumin ultimate density reaches 15 g/L, closes 24 hours under the conditions of 4 DEG C, can be prepared into
It is coated anti-insulin antibody to latex.
As preferably, the invention also discloses preparation method and the using method of the test kit of said determination insulin, bag
Include following steps:
A () prepares reagent according to following component content:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
PEG-8 000 20 ~ 60 g/L
Tween 20 10 ~ 35 g/L
Sodium azide 0.3 ~ 1.7 g/L
Sodium ethylene diamine tetracetate 1.5 ~ 6.5 mmol/L
Bovine serum albumin 10 ~ 30 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Sodium chloride 150 ~ 350 mmol/L
Sodium azide 0.3 ~ 1.7 g/L
Bovine serum albumin 10 ~ 30 g/L
Latex is coated anti-insulin antibody 1 ~ 8g/L
Its solvent is purified water；
B sample to be tested is mixed by () with reagent R1 and reagent R2 so that it is fully react；
C () measures reacted absorbance difference with automatic clinical chemistry analyzer；
D () calculates the concentration of insulin in sample according to absorbance changing value.
As preferably, in step (b), described reagent R1 and the volume ratio of reagent R2 are 2:1；
As preferably, in step (b), described sample to be tested arrives at 1:2 with the volume ratio of reagent R1 and the cumulative volume of reagent R2
Between 1:20.
The Cleaning Principle of the present invention is: the present invention utilizes antigen antibody reaction, is coated glucagon and resists on latex particle
Body, the insulin generation agglutination in insulin latex antibody and sample, form antigenantibody complex and produce one
Determining turbidity, the height of this turbidity becomes relevant to the insulin concentration in sample, by measuring absorbance and according to calibration curve
Calculate the content of insulin.
Activity (the uIU/mL)=C of insulin (INS) in sampleS ×(uIU/mL)
In formula: Δ ATThe sample cell absorbance compared with blank tube absorbance
ΔASThe calibration pipe absorbance compared with blank tube absorbance
CSThe concentration of INS in calibration solution.
Compared with prior art, the present invention has following advantageous benefits: the present invention with the addition of ethylenediamine tetraacetic in reagent R1
Sodium acetate, it is possible to effectively removing from the interference of metal ion in sample, the accuracy therefore detected is higher, and by R1
In with the addition of PEG-8 000 as accelerator, promoting is swift in response is carried out, and remolding sensitivity is higher, and that therefore detects is accurate
Property relatively good, and the present invention does not contains any radioelement, will not produce environment and pollute, and additionally operate the most more convenient.
Detailed description of the invention
Further illustrate below in conjunction with specific embodiment, but the present invention is not limited in these embodiments.
Embodiment 1
The test kit of the present invention includes reagent R1 independent of each other and reagent R2 biliquid component, wherein
Reagent R1:
Tris buffer 100 mmol/L
PEG-8 000 40 g/L
Tween 20 20 g/L
Sodium azide 1.0 g/L
Sodium ethylene diamine tetracetate 4.0 mmol/L
Bovine serum albumin 20 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 100 mmol/L
Sodium chloride 200 mmol/L
Sodium azide 1.0 g/L
Bovine serum albumin 20 g/L
Latex is coated anti-insulin antibody 4g/L
Its solvent is purified water.
Embodiment 2
The test kit of the present invention includes reagent R1 independent of each other and reagent R2 biliquid component, wherein
Reagent R1:
Tris buffer 150 mmol/L
PEG-8 000 40 g/L
Tween 20 25g/L
Sodium azide 0.3g/L
Sodium ethylene diamine tetracetate 6.5 mmol/L
Bovine serum albumin 10 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 20mmol/L
Sodium chloride 350 mmol/L
Sodium azide 1.7 g/L
Bovine serum albumin 10 g/L
Latex is coated anti-insulin antibody 3g/L
Its solvent is purified water.
Embodiment 3
The preparation and application of test kit
1, latex is coated the preparation of anti-insulin antibody: be first the polyphenyl of 120nm with the MES buffer of 50 mmol/L by particle diameter
The dilution of ethylene microsphere becomes the solution that mass concentration is 5% of contained polystyrene microsphere, then adds in every milliliter of solution
1-ethyl-3-(3-DimethylAminopropyl) carbodiimide hydrochloride of 1.0 mg, reaction 1 hour under conditions of 37 DEG C, instead
Centrifuge should be used after completing, be centrifuged 30 minutes under the rotating speed of 25000 rpm/min, remove supernatant, then by precipitation with 50
The MES buffer dilution of mmol/L, then uses ultrasonic disperse instrument to carry out ultrasonic disperse, re-uses centrifuge, 25000
It is centrifuged 30 minutes under rpm/min rotating speed, removes supernatant, the precipitation MES buffer of 50 mmol/L is diluted, until poly-in solution
Till the mass concentration of phenylethylene micro ball is 3%, ultrasonic disperse, then the addition of limit dispersion limit is isopyknic contains the 8 anti-islets of langerhans of mg/mL
The MES buffer of element antibody, mix and blend, react 2 hours under conditions of 37 DEG C, until the final mass of polystyrene microsphere
Till when concentration is 1%, it is subsequently adding bovine serum albumin so that bovine serum albumin ultimate density reaches 15 g/L, 4 DEG C of bars
Close 24 hours under part, latex can be prepared and be coated anti-insulin antibody；
2, reagent is prepared according to following component content:
Reagent R1:
Tris buffer 100 mmol/L
PEG-8 000 40 g/L
Tween 20 20 g/L
Sodium azide 1.0 g/L
Sodium ethylene diamine tetracetate 4.0 mmol/L
Bovine serum albumin 20 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 100 mmol/L
Sodium chloride 200 mmol/L
Sodium azide 1.0 g/L
Bovine serum albumin 20 g/L
Latex is coated anti-insulin antibody 4g/L
Its solvent is purified water；
3, automatic clinical chemistry analyzer parameter is arranged
(a) detection temperature: 37 DEG C；
(b) detection wavelength: dominant wavelength 600nm；
(c) response time: 10min, wherein, incubation time 5min, measure immediately after adding reagent R2 and read absorbance
Read absorbance A 2 after A1,5min, calculate absorbance changes delta A=A2-A1；
(d) the Direction of Reaction: positive reaction；
4, detecting step
A () takes 160 μ l reagent R1 and the mixing of 20 μ l samples to be tested；
B solution after mixing is hatched 5min under conditions of 37 DEG C by ()；
C () adds 80 μ l reagent R2, measure immediately and read absorbance A 1, reads absorbance A 2 after 5min, calculates absorbance change
Δ A=A2-A1；
D () is according to activity (the uIU/mL)=C of the insulin (INS) in sampleS × (uIU/mL) calculate in sample
The concentration of insulin.
Embodiment 4
The preparation and application of test kit
1, latex is coated the preparation of anti-insulin antibody: be first the polyphenyl of 120nm with the MES buffer of 50 mmol/L by particle diameter
The dilution of ethylene microsphere becomes the solution that mass concentration is 5% of contained polystyrene microsphere, then adds in every milliliter of solution
1-ethyl-3-(3-DimethylAminopropyl) carbodiimide hydrochloride of 1.0 mg, reaction 1 hour under conditions of 37 DEG C, instead
Centrifuge should be used after completing, be centrifuged 30 minutes under the rotating speed of 25000 rpm/min, remove supernatant, then by precipitation with 50
The MES buffer dilution of mmol/L, then uses ultrasonic disperse instrument to carry out ultrasonic disperse, re-uses centrifuge, 25000
It is centrifuged 30 minutes under rpm/min rotating speed, removes supernatant, the precipitation MES buffer of 50 mmol/L is diluted, until poly-in solution
Till the mass concentration of phenylethylene micro ball is 3%, ultrasonic disperse, then the addition of limit dispersion limit is isopyknic contains the 8 anti-islets of langerhans of mg/mL
The MES buffer of element antibody, mix and blend, react 2 hours under conditions of 37 DEG C, until the final mass of polystyrene microsphere
Till when concentration is 1%, it is subsequently adding bovine serum albumin so that bovine serum albumin ultimate density reaches 15 g/L, 4 DEG C of bars
Close 24 hours under part, latex can be prepared and be coated anti-insulin antibody；
2, reagent is prepared according to following component content:
Reagent R1:
Tris buffer 150 mmol/L
PEG-8 000 40 g/L
Tween 20 25g/L
Sodium azide 0.3g/L
Sodium ethylene diamine tetracetate 6.5 mmol/L
Bovine serum albumin 10 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 20mmol/L
Sodium chloride 350 mmol/L
Sodium azide 1.7 g/L
Bovine serum albumin 10 g/L
Latex is coated anti-insulin antibody 3g/L
Its solvent is purified water；
3, automatic clinical chemistry analyzer parameter is arranged
(a) detection temperature: 37 DEG C；
(b) detection wavelength: dominant wavelength 600nm；
(c) response time: 10min, wherein, incubation time 5min, measure immediately after adding reagent R2 and read absorbance
Read absorbance A 2 after A1,5min, calculate absorbance changes delta A=A2-A1；
(d) the Direction of Reaction: positive reaction；
4, detecting step
A () takes 160 μ l reagent R1 and the mixing of 20 μ l samples to be tested；
B solution after mixing is hatched 5min under conditions of 37 DEG C by ()；
C () adds 80 μ l reagent R2, measure immediately and read absorbance A 1, reads absorbance A 2 after 5min, calculates absorbance change
Δ A=A2-A1；
D () is according to activity (the uIU/mL)=C of the insulin (INS) in sampleS × (uIU/mL) calculate in sample
The concentration of insulin.
Mensuration insulin obtained by the test kit of the table 1 mensuration insulin obtained by embodiment 1 and embodiment 2
The result that quality-control product 1 is measured by test kit respectively, wherein the concentration of the insulin in quality-control product 1 is 18.14 uIU/mL,
Measurement result is shown in Table 1:
Table 1
| 1st time (uIU/mL) | 2nd time (uIU/mL) | 3rd time (uIU/mL) | Average (uIU/mL) | Deviation (%) | |
| Embodiment 1 | 18.42 | 18.46 | 18.45 | 18.44 | 1.65 |
| Embodiment 2 | 17.98 | 17.56 | 17.79 | 17.78 | 2.03 |
As shown in Table 1, the test kit measuring insulin obtained by the present invention is less to the measurement result deviation of quality-control product 1, because of
This accuracy of measurement is high, and embodiment 1 is optimum selection.
The table 2 test kit measuring insulin obtained by embodiment 1 and the reagent measuring insulin obtained by embodiment 2
The result that quality-control product 2 is measured by box respectively, wherein the concentration of the insulin in quality-control product 2 is 58.10 uIU/mL, measures
The results are shown in Table 2:
Table 2
| 1st time (uIU/mL) | 2nd time (uIU/mL) | 3rd time (uIU/mL) | Average (uIU/mL) | Deviation (%) | |
| Embodiment 1 | 58.03 | 58.65 | 57.10 | 57.93 | 0.29 |
| Embodiment 2 | 57.14 | 58.36 | 57.21 | 57.57 | 0.91 |
As shown in Table 2, the test kit measuring insulin obtained by the present invention is less to the measurement result deviation of quality-control product 2, because of
This accuracy of measurement is high, and embodiment 1 is optimum selection.
The table 3 test kit measuring insulin obtained by embodiment 3 is surveyed the most repeatedly to what same sample to be tested was carried out
The test kit measuring insulin calmly and obtained by embodiment 4 is repeatedly repeatedly measured what same sample to be tested was carried out, to institute
The result obtained carries out the calculating of SD and CV, and result is as follows:
Table 3
The precision of the test kit measuring insulin obtained by the present invention is relatively good as shown in Table 3, and as shown in Table 3, real
Executing example 3 is optimum selection.
The principle of above-described embodiment only illustrative present invention and effect thereof, not for limiting the present invention.Any ripe
Above-described embodiment all can be modified under the spirit and the scope of the present invention or change by the personage knowing this technology.Cause
This, have usually intellectual such as complete with institute under technological thought without departing from disclosed spirit in art
All equivalences become are modified or change, and must be contained by the claim of the present invention.
Claims (6)
1. the test kit measuring insulin, it is characterised in that: include reagent R1 independent of each other and reagent R2 biliquid group
Point, including composition and corresponding content be:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
PEG-8 000 20 ~ 60 g/L
Tween 20 10 ~ 35 g/L
Sodium azide 0.3 ~ 1.7 g/L
Sodium ethylene diamine tetracetate 1.5 ~ 6.5 mmol/L
Bovine serum albumin 10 ~ 30 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Sodium chloride 150 ~ 350 mmol/L
Sodium azide 0.3 ~ 1.7 g/L
Bovine serum albumin 10 ~ 30 g/L
Latex is coated anti-insulin antibody 1 ~ 8g/L
Its solvent is purified water.
A kind of test kit measuring insulin the most according to claim 1, it is characterised in that: include reagent independent of each other
R1 and reagent R2 biliquid component, including composition and corresponding content be:
Reagent R1:
Tris buffer 100 mmol/L
PEG-8 000 40 g/L
Tween 20 20 g/L
Sodium azide 1.0 g/L
Sodium ethylene diamine tetracetate 4.0 mmol/L
Bovine serum albumin 20 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 100 mmol/L
Sodium chloride 200 mmol/L
Sodium azide 1.0 g/L
Bovine serum albumin 20 g/L
Latex is coated anti-insulin antibody 4g/L
Its solvent is purified water.
A kind of test kit measuring insulin the most according to claim 1 and 2, it is characterised in that: in described reagent R2,
Described latex is coated the preparation method of anti-insulin antibody: first with the MES buffer of 50 mmol/L by particle diameter be 80 ~
The polystyrene microsphere dilution of 500nm becomes the solution that mass concentration is 1%-5% of contained polystyrene microsphere, then every milli
Rise 1-ethyl-3-(3-DimethylAminopropyl) carbodiimide hydrochloride adding 1.0 mg in solution, at the bar of 20 DEG C-37 DEG C
React 1 hour under part, use centrifuge after having reacted, be centrifuged 30 minutes under the rotating speed of 25000 rpm/min, remove supernatant,
Then by the precipitation MES buffer of 50 mmol/L dilution, then use ultrasonic disperse instrument carries out ultrasonic disperse, re-use from
Scheming, is centrifuged 30 minutes under 25000 rpm/min rotating speeds, removes supernatant, is diluted by the precipitation MES buffer of 50 mmol/L,
Till in solution, the mass concentration of polystyrene microsphere is 1%-3%, ultrasonic disperse, then dispersion limit in limit adds isopyknic
Containing the MES buffer of 8 mg/mL anti-insulin antibody, mix and blend, react 2 hours under conditions of 20 DEG C-37 DEG C, until poly-
Till when the final mass concentration of phenylethylene micro ball is 1%, it is subsequently adding bovine serum albumin so that bovine serum albumin is final
Concentration reaches 15 g/L, closes 24 hours, can prepare latex and be coated anti-insulin antibody under the conditions of 4 DEG C.
The preparation method of a kind of test kit measuring insulin the most according to claim 1 and 2 and using method, its feature
It is: comprise the following steps:
A () prepares reagent according to following component content:
Reagent R1:
Tris buffer 20 ~ 180 mmol/L
PEG-8 000 20 ~ 60 g/L
Tween 20 10 ~ 35 g/L
Sodium azide 0.3 ~ 1.7 g/L
Sodium ethylene diamine tetracetate 1.5 ~ 6.5 mmol/L
Bovine serum albumin 10 ~ 30 g/L
Its solvent is purified water
Reagent R2:
Tris buffer 20 ~ 180 mmol/L
Sodium chloride 150 ~ 350 mmol/L
Sodium azide 0.3 ~ 1.7 g/L
Bovine serum albumin 10 ~ 30 g/L
Latex is coated anti-insulin antibody 1 ~ 8g/L
Its solvent is purified water；
B sample to be tested is mixed by () with reagent R1 and reagent R2 so that it is fully react；
C () measures reacted absorbance difference with automatic clinical chemistry analyzer；
D () calculates the concentration of insulin in sample according to absorbance changing value.
The preparation method of a kind of test kit measuring insulin the most according to claim 4 and using method, its feature exists
In: in step (b), described reagent R1 and the volume ratio of reagent R2 are 2:1.
The preparation method of a kind of test kit measuring insulin the most according to claim 4 and using method, its feature exists
In: in step (b), the volume ratio of the cumulative volume of described sample to be tested and reagent R1 and reagent R2 is between 1:2 to 1:20.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107576806A (en) * | 2017-09-30 | 2018-01-12 | 安徽伊普诺康生物技术股份有限公司 | A kind of granulocyte colony stimulating factor detection kit and its application method |
| CN107748251A (en) * | 2017-09-30 | 2018-03-02 | 安徽伊普诺康生物技术股份有限公司 | A kind of preparation method of granulocyte colony stimulating factor detection kit |
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| CN102818902A (en) * | 2012-08-16 | 2012-12-12 | 北京恩济和生物科技有限公司 | Detection kit for insulin and preparation method thereof |
| CN103073642A (en) * | 2012-08-29 | 2013-05-01 | 深圳伯美生物医药有限公司 | CRP monoclonal antibody nanometer latex microsphere composition and preparation process thereof |
| CN104215769A (en) * | 2014-08-14 | 2014-12-17 | 上海睿康生物科技有限公司 | Latex enhanced immunoturbidimetry NGAL detection kit |
| CN104237498A (en) * | 2013-06-13 | 2014-12-24 | 绍兴圣康生物科技有限公司 | Preparation method of insulin determination kit employing latex enhanced turbidimetric immunoassay |
| CN104360056A (en) * | 2014-12-05 | 2015-02-18 | 重庆中元生物技术有限公司 | Method for improving sensitivity of latex enhanced turbidimetric immunoassay |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102818902A (en) * | 2012-08-16 | 2012-12-12 | 北京恩济和生物科技有限公司 | Detection kit for insulin and preparation method thereof |
| CN103073642A (en) * | 2012-08-29 | 2013-05-01 | 深圳伯美生物医药有限公司 | CRP monoclonal antibody nanometer latex microsphere composition and preparation process thereof |
| CN104237498A (en) * | 2013-06-13 | 2014-12-24 | 绍兴圣康生物科技有限公司 | Preparation method of insulin determination kit employing latex enhanced turbidimetric immunoassay |
| CN104215769A (en) * | 2014-08-14 | 2014-12-17 | 上海睿康生物科技有限公司 | Latex enhanced immunoturbidimetry NGAL detection kit |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107576806A (en) * | 2017-09-30 | 2018-01-12 | 安徽伊普诺康生物技术股份有限公司 | A kind of granulocyte colony stimulating factor detection kit and its application method |
| CN107748251A (en) * | 2017-09-30 | 2018-03-02 | 安徽伊普诺康生物技术股份有限公司 | A kind of preparation method of granulocyte colony stimulating factor detection kit |
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Application publication date: 20161026 |
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