CN106037669A - Finger bio-photon emission spectral distribution graph drawing device and method thereof - Google Patents
Finger bio-photon emission spectral distribution graph drawing device and method thereof Download PDFInfo
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0064—Body surface scanning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention discloses a finger bio-photon emission spectral distribution graph drawing device and a method thereof. The device comprises a darkroom; holes are formed in the front face of the darkroom; photomultiplier tubes are vertically arranged right above the darkroom; an electrically controlled filter wheel is arranged between the darkroom and the photomultiplier tubes; and a plurality of groups of filters are loaded on the filter wheel. Bio-photons spontaneously generated by a finger extending into the darkroom are transmitted to the photomultiplier tubes after passing through one group of filter and then converted into bio-electricity signals, and the bio-electricity signals obtained after conversion are transmitted to a photon counter by the photomultiplier tubes, and the luminous intensity of the finger is obtained by the photon counter; when the filter is non-loaded, the luminous intensity of the filter is obtained; and the photon counter is also connected with a central processing unit, and the central processing unit is used for receiving the luminous intensity of the finger and the luminous intensity of the filter, and calculating the difference between the luminous intensity of the finger and the luminous intensity of the filter to obtain a finger self-luminous intensity, and obtaining a finger spectral distribution graph according to the finger self-luminous intensity.
Description
Technical field
The invention belongs to biophoton emission field of detecting, particularly relate to a kind of finger biometric photon radiation spectral distribution graph
Drawing apparatus and method thereof.
Background technology
Biophoton emission during deriving from body intracellular metabolic various biochemical reactions from upper state to lower state
The photon that transition is launched, it is the generation biosis at " molecular level ", and it is that organism itself is intrinsic
Attribute, is an inherent function of biosystem, carries structure change and functional metabolism shape in body metabolic processes
The information of state, this information lies in the middle of its parameters such as intensity, spectral distribution.Biosystem is in the change of molecular level
Change, can cause system biological photon radiation in the change of the aspect such as intensity, spectral distribution, it to the change within biosystem and
The impact of external environment has the sensitivity of height, and detection and analysis to body superweak luminescence can disclose inside biosystem
Details, show external environment minor way.
The spontaneous biophoton emission of human finger is found in the seventies in last century, but currently without just for human finger
Spontaneous biophoton emission draws the device of its radiation spectrum profiles.Additionally, due to the radiation of finger spontaneous photon is the most micro-
Weak, the device sensitivity of the existing spontaneous bio-photon of detection human finger is low, operation is complicated and poor stability, even endangers people
The health of body finger.
Summary of the invention
In order to solve the shortcoming of prior art, the present invention provides a kind of finger biometric photon radiation spectral distribution graph to draw dress
Put and method.This device sensitivity is high, simple to operate, it is possible to indicates the state of health intuitively and has quick, clever
Quick, reliable and lossless to human finger advantage.
For achieving the above object, the present invention is by the following technical solutions:
A kind of finger biometric photon radiation spectral distribution graph drawing apparatus, including darkroom, the front in described darkroom is provided with
Stretching into the hole of staff, the surface in described darkroom is vertically installed with photomultiplier tube, between described darkroom and photomultiplier tube
It is also equipped with filter plate wheel, described filter plate wheel carries the filter plate of some groups of different-wavebands;
Filter plate is for when there being finger in darkroom, the bio-photon of generation spontaneous to the finger in darkroom is filtered,
And filtered bio-photon be sent to photomultiplier tube carry out being converted into bioelectrical signals, the bioelectrical signals after conversion by
Photomultiplier tube is sent to photon counter, obtains finger luminous intensity under arbitrary group of filter plate;
Described filter plate is additionally operable to when in darkroom without finger, and self-luminous produces optical signal, and light self-luminous produced
Signal is sent to photon counter by photomultiplier tube, obtains the luminous intensity of arbitrary group of filter plate self;
Described photon counter is also connected with central processing unit, and described central processing unit is used for receiving finger and filters at arbitrary group
Luminous intensity under wave plate and the luminous intensity of arbitrary group of filter plate self, and by strong for the luminescence of the finger under same group of filter plate
Degree is poor with the luminous intensity of filter plate self, obtains finger self-luminous intensity under specific band, and then draws out finger
Spectral distribution graph.
Described hole outer is fixed with black rubber set, for preventing the entrance of ambient.
Described filter plate wheel is the filter plate wheel of Electronic control.
Described finger biometric photon radiation spectral distribution graph drawing apparatus also includes that high voltage power supply, described high voltage power supply are used for
Power supply is provided for photomultiplier tube.
The front in described darkroom is provided with dividing plate in being provided with two circular cavities and darkroom, for realizing right-hand man's bio-light
Son detects simultaneously.
Described central processing unit is also connected with display screen.
A kind of method of work of finger biometric photon radiation spectral distribution graph drawing apparatus, including:
Stretching in darkroom by hands by hole, the bio-photon of each finger of opponent is tested:
In test process, the bio-photon of the spontaneous generation of finger in darkroom, after arbitrary group of filter plate filtering, passes
Delivering to photomultiplier tube and be converted into bioelectrical signals, the bioelectrical signals after conversion is sent to photon counting by photomultiplier tube
Device, the bioelectrical signals received is processed by photon counter, obtains finger luminous intensity under arbitrary group of filter plate;
When in darkroom without finger, the optical signals photomultiplier tube of arbitrary group of filter plate self-luminous generation is sent to photon
After enumerator, obtain the luminous intensity of arbitrary group of filter plate self;
By poor to the luminous intensity of the finger under same group of filter plate and the luminous intensity of filter plate self, obtain finger and exist
Self-luminous intensity under specific band, and then draw out finger spectral distribution graph.
After obtaining finger self-luminous intensity under specific band, also include according to different filter plates in different-waveband
Quantum efficiency finger self-luminous intensity under specific band is corrected.
Before the method also includes by hole, hands is stretched into darkroom, temperature regulation is controlled to 20 DEG C and ambient humidity
Between 40%-50%.This is done to ensure the accuracy of detection finger self-luminous intensity under specific band.
The invention have the benefit that
(1) front in the darkroom of the present invention is provided with dividing plate in being provided with two circular cavities and darkroom, forms double darkroom, is used for
Realize right-hand man's bio-photon is detected simultaneously;And the present invention uses high sensitivity and the photoelectricity times without additional refrigeration
Increase the bio-photon that pipe detects each finger of hands, improve the measuring accuracy of whole device and make simple to operate, this
Invention can indicate the state of health intuitively and have quick, sensitive, reliable and lossless to human finger advantage.
(2) present invention uses the photomultiplier tube of the surface being vertically mounted on darkroom and is arranged on filter plate wheel
The filter plate of some groups of different-wavebands, detects the luminous intensity under arbitrary group of filter plate of the finger in darkroom and arbitrary group of filter
The luminous intensity of wave plate self, and by the luminous intensity of the luminous intensity of the finger under same group of filter plate Yu filter plate self
Differ from, obtain finger self-luminous intensity under specific band, and then draw out finger spectral distribution graph exactly.
Accompanying drawing explanation
Fig. 1 is assembly of the invention structural representation;
Fig. 2 is the method for work schematic flow sheet of assembly of the invention;
Fig. 3 (a) is the distribution of Healthy People left-hand finger spontaneous photon radiation spectrum;
Fig. 3 (b) Healthy People right finger spontaneous photon radiation spectrum is distributed;
Fig. 3 (c) is as a example by cold patients, left-hand finger spontaneous photon radiation spectrum distribution under morbid state;
Fig. 3 (d) is as a example by cold patients, right finger spontaneous photon radiation spectrum distribution under morbid state.
Wherein, 1, darkroom;2, hole;3, photomultiplier tube;4, filter plate wheel;5, photon counter;6, high voltage power supply;7、
Central processing unit.
Detailed description of the invention
The present invention will be further described with embodiment below in conjunction with the accompanying drawings:
As it is shown in figure 1, the finger biometric photon radiation spectral distribution graph drawing apparatus of the present invention includes darkroom 1, photoelectricity times
Increase pipe 3, filter plate wheel 4 and photon counter 5.
Wherein, front, darkroom 1 is provided with the hole stretching into staff, and the surface in darkroom 1 is vertically installed with photomultiplier tube
3, it is also equipped with filter plate wheel 4 between described darkroom 1 and photomultiplier tube 3, described filter plate wheel 4 carries some groups of differences
The filter plate of wave band.
Darkroom 1 is the important component part of both hands photon detection system, and it acts on is that the measurement for hand spontaneous photon carries
For the environment of complete darkness, it is divided into two the dark lattice in left and right, it is simple to right-hand man detects simultaneously.Owing to the photon of hand radiation is the most micro-
Weak, therefore the light seal in darkroom is the best, and to avoid the impact of external stray light, this experiment darkroom used is according to hand
Luminous that need custom-made, its inwall and periphery are all through blackening process, such as: to whole darkroom surfaces externally and internally all with pitch-dark
Even application.
In the present embodiment, front, darkroom 1 has the circular opening 2 of two a diameter of 10cm, hole 2 outer to be respectively fixed with
The black rubber glove of long 25cm, its effect be to ensure that arm after hole stretches into darkroom, the contact position, hole of arm and hole 2
Entrance without ambient;Vertically equipped with two photomultiplier tubes 3 directly over darkroom 1, at darkroom 1 upper surface and photomultiplier tube
Take turns 4 equipped with automatically controlled filter plate between 3, built with five groups of filter plates, five groups of filter plates be respectively GG395, GG455, GG495,
OG550 and RG610, for the measurement of hand spontaneous photon radiation spectrum.
Photomultiplier tube 3 is the core component of both hands spontaneous photon detecting system, and the height of its sensitivity determines whole
The system detectivity to hand faint light.Photomultiplier tube 3 (PMT) is a kind of faint optical signal to be converted into and can be visited
The electrooptical device of the signal of telecommunication surveyed, it is received by photo-emissive cathode (photocathode), focusing electrode, dynode and electronics
Collectors (anode) etc. form, and its operation principle is when low light irradiation to photocathode, and photocathode inspires photoelectron, these photoelectricity
Son is pressed focusing electrode electric field and is entered dynode system, and the multiplication obtained by further Secondary Emission is amplified, then after amplification
Electronics with anode collect as signal export.Photomultiplier tube of the present invention is that Britain ET Enterprises produces
9235QA type: diameter 51mm (2-inch), end window type is incident, bialkali photocathode, effective photocathode diameter 48mm, wave spectrum
Response range is 290nm-630nm, has the features such as high-gain, quick time response, low dark count rate.This device is used up
Electricity multiplier tube, without refrigeration, i.e. use of starting shooting, is greatly saved the time that test waits.
Wherein, filter plate is for when having finger in darkroom 1, and the bio-photon of generation spontaneous to the finger in darkroom 1 enters
Row filtering, and filtered bio-photon is sent to photomultiplier tube carries out being converted into bioelectrical signals, the biology after conversion
The signal of telecommunication is sent to photon counter 5 by photomultiplier tube 3, obtains finger luminous intensity under arbitrary group of filter plate;
Filter plate is additionally operable to when in darkroom without finger, and self-luminous produces optical signal, and optical signal self-luminous produced
It is sent to photon counter 5 by photomultiplier tube 3, obtains the luminous intensity of arbitrary group of filter plate self.
The photon counter 5 used in the present embodiment is the C9744 type that Hamamatsu company of Japan produces, such as Fig. 3 institute
Show.This type photon counter 5 can be converted into the single photoelectron in photomultiplier tube 3 by incorporated amplifier and discriminator circuit
The digital signal of 5V shows.C9744 type photon counter has high-velocity electrons circuit, can realize the measurement of 107s-1, have ÷
10 and ÷ 1 two grades, wherein one grade can be selected according to the intensity of measured signal.
Photon counter 5 is also connected with central processing unit 7, and described central processing unit 7 is used for receiving finger and filters at arbitrary group
Luminous intensity under wave plate and the luminous intensity of arbitrary group of filter plate self, and by strong for the luminescence of the finger under same group of filter plate
Degree is poor with the luminous intensity of filter plate self, obtains finger self-luminous intensity under specific band, and then draws out finger
Spectral distribution graph.
Wherein, finger biometric photon radiation spectral distribution graph drawing apparatus also includes high voltage power supply, and described high voltage power supply is used
In providing power supply for photomultiplier tube.High voltage power supply in the present embodiment is the PM20SP that Sens-Tech company produces, its effect
It is to provide the most adjustable, stable and almost without ripple High voltage output for photomultiplier tube.PM20SP type high voltage power supply passes through one
Potentiometer is read in the accurate dialing of individual 10 turns, and to realize output the most adjustable from 0-maximum, has that noise is low, stability is high and short-term
The feature of drift.
In specific implementation process, central processing unit is also connected with display screen, and display screen draws out finger for display directly perceived
Spectral distribution graph.
The specific works flow process of the finger biometric photon radiation spectral distribution graph drawing apparatus of the present invention is:
(1) prepare before test.Within two hours, opening laboratory air-conditioning in advance, temperature regulates to 20 DEG C, and ambient humidity controls
Between 40%-50%.Due to this experiment to be obtained be hand spontaneous photon radiation, therefore experiment before need to carry out secretly hand
Adaptation processes to mask the ambient impact on skin delayed luminescence.Before experiment, experimenter must be with normal saline or tap water
Hand is cleaned up, after naturally drying, puts on preprepared black glove, quiet wait 30 minutes immediately.
(2) parameter is arranged.Testing results software, now can hear filter plate wheel start rotate, system starts self-inspection, wait be
System self-inspection completes, and completes the setting of relevant parameter at operation interface.
(3) test process:
1. after startup self-detection process terminates, before the radiation test of finger spontaneous photon, it is necessary first in test experiments darkroom
Noise and the spontaneous fluorescence intensity of different filter plate self.Parameter is arranged ibid.
2., after the dark adaptation time of 30 minutes has arrived, experimenter adjusts posture and sits up straight before experiment porch;
3. the both hands having on black glove are put in experiment magazine, by under glove picking in experiment magazine, it is therefore an objective to keep away
Exempt from hand again by external light influence;
4. being respectively placed in by corresponding for right-hand man finger fingertip to be measured fixes at groove immediately below left-right photo multiplier tube camera lens,
Then clicking on " Measure ", start counting up, now filter plate state is in " No filter ", a finger tip be completed after more
Change another root finger.Finger measuring sequence is as follows: the thumb → forefinger → middle finger → third finger → little finger of toe;
5., after the 3rd pacing amount, change filter plate, obtain GG395, GG455, GG495 successively by above-mentioned measuring method,
Measurement data under OG550, RG610 difference filter plate;
6. being measured, data derive, analyzing and processing;
(4) data process.As a example by thumb, first the thumb spontaneous luminescence intensity recorded is deducted making an uproar of filter plate itself
Sound, i.e. (finger spontaneous photon intensity+filter plate luminous intensity)-filter plate luminous intensity, obtain thumb under different filter plates
The luminous intensity of itself, after secondly the light intensity under previous filter plate deducts, the light intensity under a filter plate obtains between two wavelength
Finger photon intensity, finally combines different filter plate quantum efficiency in different-waveband, is corrected the numerical value between wave band,
Spectral distribution graph is drawn in units of 100nm.
(5) test result.
During can judging body intracellular metabolic on the whole by human body spontaneous luminescent spectrum feature, oxidation is freely
The level of base, thus evaluate the health status of body.Physics and the principles of chemistry research of bioluminescence are thought, only sharp when occurring
Send out state molecule when ground state transition, just have luminescence phenomenon, in other words, archebiosis light sources is in biomacromolecule new old generation
From upper state to the photon of lower state transition radiation, the photon that interior free yl isoreactivity oxygen is discharged, available body during thanking
Table superweak luminescence characterizes.Photon emission with metabolism mechanism is thought, intracellular highly active peroxylradicals carry out chemically composited instead
During Ying, generate singlet oxygen or excited state carbonyl, when they return to ground state, then give off the photon of different-waveband.In a large number
Experimentation show, be embedded in the membrane bound enzyme complex NADPH of cell membrane, mitochondrial membrane, chloroplast membranes, phagosome film etc.
Oxidase can be by oxygen (O2) it is converted into ultra-oxygen anion free radical (O2 ·-), O2 ·-Hydrogen peroxide is generated by dismutation reaction
(H2O2), H2O2React through Fenton and then change into hydroxyl radical free radical (HO·).There is the HO of higher reproducibility·Thin by oxidation
Intracellular includes various types of biomacromolecules of lipid, protein and nucleic acid, ultimately generate triplet excited state carbonyl (3R=
O*), substance (1P*) and triple (3P*) excited state pigment and singlet oxygen (1O2).Triplet excited state carbonyl (3R=O*) from upper state
To lower state transition produce 350-550nm wave band photon, substance (1P*) and triple (3P*) excited state pigment transition correspondence respectively
550-750nm and 750-1000nm wave band, singlet oxygen transition produces the photon of 634nm and 1270nm wave band.
When body is in morbid state, spectrally can embody at finger spontaneous luminescence.Finger spontaneous luminescence spectrum
The peak feature (such as peak intensity, peak position, the quantity at peak) of distribution is possibly as the characteristic index of body metabolic activity, logical
Cross test and analyze finger spontaneous luminescence spectral characteristic under different physio-pathological condition, comparing the difference of its spectral distribution feature,
I.e. can determine whether the health status of body.
Under health status shown in human body right-hand man finger spectral distribution such as Fig. 3 (a) and Fig. 3 (b).Under morbid state, human body is left
Shown in right finger spectral distribution such as Fig. 3 (c) and Fig. 3 (d).From such as Fig. 3 (a) and Fig. 3 (b) it can be seen that ten handss of Healthy People
Referring to that spontaneous luminescence strength is apparently higher than other wave band between 495-550nm, prompting health status people ten refers to have relatively at this wave band
Strong photon radiation, the i.e. peak value of Healthy People ten finger tip spectral distribution occur between 495-550nm.At this section, every
The light intensity numerical value of finger is the most different, shows as left hand and is followed successively by thumb, middle finger, forefinger, little finger of toe and the third finger from high to low,
The right hand is followed successively by thumb, middle finger, little finger of toe, forefinger and the third finger.
From Fig. 3 (c) and Fig. 3 (d) it can be seen that refer to that spectral distribution is compared with Healthy People ten, in the right-hand man of cold patients
Refer to, nameless and little finger of toe spectrum peak occurs in that Red Shift Phenomena, i.e. spectrum peak moves to 550-610nm from 495-550nm, fixed
From the point of view of Liang, i.e. cold patients right-hand man's middle finger, the third finger and little finger of toe light intensity is obvious at the ratio of 550-610nm with 495-550nm
Higher than Healthy People (left hand: p=0.007;The right hand: p=0.005).Test result shows, is in the human body of morbid state, and it is raw
The distribution of object light sub-radiation spectrum deviates with normal value.
The front in the darkroom of the present invention is provided with dividing plate in being provided with two circular cavities and darkroom, form double darkroom, for real
Now right-hand man's bio-photon is detected simultaneously;And the present invention uses high sensitivity and the photomultiplier transit without additional refrigeration
Pipe detects the bio-photon of each finger of hands, improves the measuring accuracy of whole device and makes simple to operate, this
The bright state that can indicate health intuitively and there is quick, sensitive, reliable and lossless to human finger advantage.
If the present invention uses the photomultiplier tube of the surface being vertically mounted on darkroom and is arranged on filter plate wheel
The filter plate of dry group different-waveband, detects the luminous intensity under arbitrary group of filter plate of the finger in darkroom and arbitrary group of filtering
The luminous intensity of sheet self, and the luminous intensity of the luminous intensity of the finger under same group of filter plate with filter plate self is made
Difference, obtains finger self-luminous intensity under specific band, and then draws out finger spectral distribution graph exactly.
Although the detailed description of the invention of the present invention is described by the above-mentioned accompanying drawing that combines, but not the present invention is protected model
The restriction enclosed, one of ordinary skill in the art should be understood that on the basis of technical scheme, and those skilled in the art are not
Need to pay various amendments or deformation that creative work can make still within protection scope of the present invention.
Claims (9)
1. a finger biometric photon radiation spectral distribution graph drawing apparatus, it is characterised in that including darkroom, described darkroom is just
Face is provided with the hole stretching into staff, and the surface in described darkroom is vertically installed with photomultiplier tube, described darkroom and photoelectricity times
Increase and between pipe, be also equipped with filter plate wheel, described filter plate wheel carries the filter plate of some groups of different-wavebands;
Filter plate is for when there being finger in darkroom, the bio-photon of generation spontaneous to the finger in darkroom is filtered, and will
Filtered bio-photon is sent to photomultiplier tube and carries out being converted into bioelectrical signals, and the bioelectrical signals after conversion is by photoelectricity
Multiplier tube is sent to photon counter, obtains finger luminous intensity under arbitrary group of filter plate;
Described filter plate is additionally operable to when in darkroom without finger, and self-luminous produces optical signal, and optical signal self-luminous produced
It is sent to photon counter by photomultiplier tube, obtains the luminous intensity of arbitrary group of filter plate self;
Described photon counter is also connected with central processing unit, and described central processing unit is used for receiving finger at arbitrary group of filter plate
Under luminous intensity and the luminous intensity of arbitrary group of filter plate self, and by the luminous intensity of the finger under same group of filter plate with
The luminous intensity of filter plate self is poor, obtains finger self-luminous intensity under specific band, and then draws out finger spectrum
Scattergram.
2. a kind of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 1, it is characterised in that described
Hole outer is fixed with black rubber set, for preventing the entrance of ambient.
3. a kind of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 1, it is characterised in that described
Filter plate wheel is the filter plate wheel of Electronic control.
4. a kind of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 1, it is characterised in that described
Finger biometric photon radiation spectral distribution graph drawing apparatus also includes high voltage power supply, and described high voltage power supply is used for as photomultiplier tube
Power supply is provided.
5. a kind of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 1, it is characterised in that described
The front in darkroom is provided with dividing plate in being provided with two circular cavities and darkroom, examines right-hand man's bio-photon for realization simultaneously
Survey.
6. a kind of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 3, it is characterised in that described
Central processing unit is also connected with display screen.
7. the work side of the finger biometric photon radiation spectral distribution graph drawing apparatus as described in claim 1-6 is arbitrary
Method, it is characterised in that including:
Stretching in darkroom by hands by hole, the bio-photon of each finger of opponent is tested:
In test process, the bio-photon of the spontaneous generation of finger in darkroom, after arbitrary group of filter plate filtering, is sent to
Photomultiplier tube is also converted into bioelectrical signals, and the bioelectrical signals after conversion is sent to photon counter by photomultiplier tube,
The bioelectrical signals received is processed by photon counter, obtains finger luminous intensity under arbitrary group of filter plate;
When in darkroom without finger, the optical signals photomultiplier tube of arbitrary group of filter plate self-luminous generation is sent to photon counting
After device, obtain the luminous intensity of arbitrary group of filter plate self;
By poor to the luminous intensity of the finger under same group of filter plate and the luminous intensity of filter plate self, obtain finger specific
Self-luminous intensity under wave band, and then draw out finger spectral distribution graph.
8. the method for work of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 7, its feature exists
In, after obtaining finger self-luminous intensity under specific band, also include according to different filter plate amounts in different-waveband
Finger self-luminous intensity under specific band is corrected by sub-efficiency.
9. the method for work of finger biometric photon radiation spectral distribution graph drawing apparatus as claimed in claim 7, its feature exists
In, before the method also includes by hole, hands is stretched into darkroom, temperature regulation is controlled to 20 DEG C and ambient humidity
Between 40%-50%.
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