CN106035992A - Formula and preparation method of enterococcus faecalis mini pills capable of resisting high temperature and being released in site-oriented manner - Google Patents
Formula and preparation method of enterococcus faecalis mini pills capable of resisting high temperature and being released in site-oriented manner Download PDFInfo
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- 241000194032 Enterococcus faecalis Species 0.000 title claims abstract description 54
- 229940032049 enterococcus faecalis Drugs 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims description 10
- 239000006187 pill Substances 0.000 title abstract 8
- 238000009472 formulation Methods 0.000 title 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 104
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 52
- 239000004310 lactic acid Substances 0.000 claims abstract description 52
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 239000004925 Acrylic resin Substances 0.000 claims abstract description 31
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 18
- 229920001353 Dextrin Polymers 0.000 claims abstract description 16
- 239000004375 Dextrin Substances 0.000 claims abstract description 16
- 229920002472 Starch Polymers 0.000 claims abstract description 16
- 235000019425 dextrin Nutrition 0.000 claims abstract description 16
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- 239000008267 milk Substances 0.000 claims abstract description 16
- 210000004080 milk Anatomy 0.000 claims abstract description 16
- 235000019698 starch Nutrition 0.000 claims abstract description 16
- 239000008107 starch Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 14
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 14
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 14
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 238000000576 coating method Methods 0.000 claims abstract description 13
- 239000011248 coating agent Substances 0.000 claims abstract description 12
- 241000194036 Lactococcus Species 0.000 claims description 36
- 239000008187 granular material Substances 0.000 claims description 20
- 239000008215 water for injection Substances 0.000 claims description 15
- 239000011347 resin Substances 0.000 claims description 9
- 229920005989 resin Polymers 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
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- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims 1
- 238000005243 fluidization Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 210000002429 large intestine Anatomy 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 4
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- 244000144972 livestock Species 0.000 abstract description 3
- 244000144977 poultry Species 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 241000282887 Suidae Species 0.000 abstract 1
- 239000002355 dual-layer Substances 0.000 abstract 1
- 235000013861 fat-free Nutrition 0.000 abstract 1
- 238000002347 injection Methods 0.000 abstract 1
- 239000007924 injection Substances 0.000 abstract 1
- 210000000813 small intestine Anatomy 0.000 abstract 1
- 210000002784 stomach Anatomy 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 28
- 230000000968 intestinal effect Effects 0.000 description 22
- 239000012530 fluid Substances 0.000 description 16
- 238000004090 dissolution Methods 0.000 description 10
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- 241001478240 Coccus Species 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 235000021590 normal diet Nutrition 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
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- 206010012735 Diarrhoea Diseases 0.000 description 3
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- 210000000481 breast Anatomy 0.000 description 3
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- 235000013305 food Nutrition 0.000 description 3
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- 235000021053 average weight gain Nutrition 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229930182843 D-Lactic acid Natural products 0.000 description 1
- JVTAAEKCZFNVCJ-UWTATZPHSA-N D-lactic acid Chemical compound C[C@@H](O)C(O)=O JVTAAEKCZFNVCJ-UWTATZPHSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a formula of enterococcus faecalis mini pills capable of resisting high temperature and being release in a site-oriented manner. The enterococcus faecalis mini pills are made from the following raw materials in parts by weight: 10-50 parts of lactic acid enterococcus faecalis, 35-70 parts of starch, 5-10 parts of nonfat dry milk powder, 5-15 parts of microcrystalline cellulose, 3-8 parts of dextrin, 5-15 parts of No.II polyacrylic resin, 5-15 parts of No.III polyacrylic resin, 100-200 parts of 95% alcohol, 2-5 parts of talcum powder and 20-60 parts of injection water. The enterococcus faecalis mini pills made through the formula disclosed by the invention can be resistant to high temperature of 95 DEG C for 3 minutes, and can be used as raw materials of granulated feeds for use; the enterococcus faecalis mini pills adopt an enteric dual-layer coating technique, so that the enterococcus faecalis mini pills are not released in the stomach, and the influence of gastric acid on the lactic acid enterococcus faecalis is avoided; besides, the enterococcus faecalis mini pills can be released in rear sections of small intestines and large intestines in the site-oriented manner, the quantity of the lactic acid enterococcus faecalis in the sections of the large intestines is increased, the growth of escherichia coli is restrained, the digestive absorption functions of the sections of the large intestines are improved, the occurrence of diseases is reduced, and the enterococcus faecalis mini pills are suitable for being used for breeding of various livestock and poultry and particularly breeding of pigs, have broad application prospects and are suitable for popularization and application.
Description
Technical field
The present invention relates to field of feed additive technology, specifically, refer to a kind of can be high temperature resistant and can pinpoint and release
Lactococcus micropill formula put and preparation method thereof.
Background technology
Lactic acid enterococcus faecalis is the probiotic bacteria allowing in a kind of feed additive catalogue to be added in feedstuff, belongs to amphimicrobian
Bacterium, its optimum growth temp is 35-38 DEG C, and suitable acid-base value is 5.0-7.5, can survive 30 minutes at 65 DEG C, because of lactic acid excrement intestinal
Growing environment is required relatively low by coccus, and passes on fast (20 minutes 1 generations), is widely used the most at present.Lactic acid excrement intestinal ball
Bacterium can improve intestinal microbial population balance, produces antibiotic substance, suppresses harmful bacteria growth and breeding, reduces the generation of intestinal tract disease；Simultaneously
Feedstuff, decomposition of protein, carbohydrate can be softened, produce aminoacid, lactic acid etc., can promote that calcium, phosphorus absorb, improve intestinal digestion
Absorption function and improving food conversion ratio, promotes growth of animal；Because of the disease-resistant growth-promoting function that it is outstanding, it is widely used at present
In livestock and poultry cultivation industry, it it is the green feed additive of nontoxic residue-free.
Although lactic acid enterococcus faecalis toleration is preferable, common product is resistant to 65 DEG C 30 minutes.But because of feed manufacturing process
In, granulated feed swelling temperature is more than 90 DEG C, and general enterococcus faecalis powder is not resistant to, and therefore cannot function as feed factory granule
Feedstuff uses.Lactic acid enterococcus faecalis is lactic acid bacteria class simultaneously, and its growth and breeding can produce lactic acid, when conditioning acid is low,
The vigor of meeting lactic acid bacteria inhibiting, therefore general lactobacillus powder oral administration uses, because of acid low (pH is less than 2) environment, meeting in gastric acid
Cause lactic acid enterococcus faecalis vigor to decline, affect the using effect of lactic acid bacteria.
Summary of the invention
For the problems referred to above overcoming prior art to exist, the present invention provides a kind of energy high temperature resistant and can pinpoint the lactic acid of release
Coccus micropill formula.
To achieve these goals, the technical solution used in the present invention is as follows:
A kind of energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, by following raw material system by weight
Become:
Lactic acid enterococcus faecalis 10-50 part, starch 35-70 part, defatted milk powder 5-10 part, microcrystalline Cellulose 5-15 part, dextrin
3-8 part, polyacrylic resin II 5-15 part, polyacrylic resin III 5-15 part, 95% ethanol 100-200 part, Pulvis Talci
2-5 part, water for injection 20-60 part.
Specifically, this Lactococcus micropill formula is made up of following raw material by weight:
Lactic acid enterococcus faecalis 20 parts, starch 65 parts, defatted milk powder 5 parts, microcrystalline Cellulose 10 parts, 5 parts of dextrin, polyacrylic acid
Resin No. II 6 parts, polyacrylic resin No. III 9 parts, 95% ethanol 150 parts, Pulvis Talci 2 parts, water for injection 35 parts.
More specifically, this Lactococcus micropill formula is made up of following raw material by weight:
Lactic acid enterococcus faecalis 40 parts, starch 56 parts, defatted milk powder 8 parts, microcrystalline Cellulose 14 parts, 5 parts of dextrin, polyacrylic acid
Resin No. II 6 parts, polyacrylic resin No. III 9 parts, 95% ethanol 150 parts, Pulvis Talci 2 parts, water for injection 20 parts.
Preferably, the colony counts of described lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
Based on above-mentioned formula, present invention also offers this energy high temperature resistant and the Lactococcus micropill formula of release can be pinpointed
Preparation method, comprises the steps:
(1) the lactic acid enterococcus faecalis of corresponding weight portion, starch, defatted milk powder, micro-is taken by described Lactococcus micropill formula
Crystalline cellulose, dextrin, water for injection mix homogeneously, use extrusion spheronization machine to make micropill granule, and used by this micropill granule
Fluid bed dryer is dried to moisture less than 6% below 40 DEG C, standby；
(2) polyacrylic resin II of corresponding weight portion, polyacrylic resin are taken by described Lactococcus micropill formula
No. III, 95% ethanol, Pulvis Talci, soak after polyacrylic resin II and polyacrylic resin III are pulverized and be dissolved in
In 95% ethanol, it is subsequently adding Pulvis Talci, stirs, and use 80 mesh screen, take liquid standby；
(3) the micropill granule that step (1) prepares being added fluidized-bed coating machine, the solution using step (2) to prepare is carried out
Spray coating；
(4) after solution has been sprayed, continue to dry to moisture less than 5% by the micropill granule of coating, prepare described breast
Acid coccus micropill.
Compared with prior art, the method have the advantages that
The Lactococcus micropill that the present invention makes is resistant to 95 DEG C of high temperature 3 minutes, can make as pellet raw material
With, it uses enteric solubility double-layer coatings technology, does not discharges, it is to avoid the gastric acid impact on lactic acid enterococcus faecalis, simultaneously its
Fixed point can discharge at small intestinal back segment and large intestine, improve the lactic acid enterococcus faecalis quantity of big intestinal segment, suppress Escherichia coli Growth, improve
Digestive and absorptive functions and the minimizing disease of big intestinal segment occur, and are suitable in all kinds of livestock and poultry cultivations using, especially Swine cultivation, have
It is widely applied prospect, is suitable for popularization and application.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, and embodiments of the present invention include but not limited to following reality
Execute example.
Embodiment 1
This example energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, by following raw material system by weight
Become:
Lactic acid enterococcus faecalis 20 parts, starch 65 parts, defatted milk powder 5 parts, microcrystalline Cellulose 10 parts, 5 parts of dextrin, polyacrylic acid
Resin No. II 6 parts, polyacrylic resin No. III 9 parts, 95% ethanol 150 parts, Pulvis Talci 2 parts, water for injection 35 parts.Wherein institute
The colony counts stating lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
Preparation method: (1) takes the lactic acid enterococcus faecalis of corresponding weight portion, starch, defatted milk by described Lactococcus micropill formula
Powder, microcrystalline Cellulose, dextrin, water for injection mix homogeneously, use extrusion spheronization machine to make micropill granule, and by this micropill granule
Fluid bed dryer is used to be dried below 40 DEG C to moisture less than 6%, standby；
(2) polyacrylic resin II of corresponding weight portion, polyacrylic resin are taken by described Lactococcus micropill formula
No. III, 95% ethanol, Pulvis Talci, soak after polyacrylic resin II and polyacrylic resin III are pulverized and be dissolved in
In 95% ethanol, it is subsequently adding Pulvis Talci, stirs, and use 80 mesh screen, take liquid standby；
(3) the micropill granule that step (1) prepares being added fluidized-bed coating machine, the solution using step (2) to prepare is carried out
Spray coating；
(4) after solution has been sprayed, continue to dry to moisture less than 5% by the micropill granule of coating, prepare described breast
Acid coccus micropill.
This Lactococcus micropill can generate pellet application directly as feedstuff, or is added into by a certain percentage
Normal diet uses.
Embodiment 2
This example energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, by following raw material system by weight
Become:
Lactic acid enterococcus faecalis 40 parts, starch 56 parts, defatted milk powder 8 parts, microcrystalline Cellulose 14 parts, 5 parts of dextrin, polyacrylic acid
Resin No. II 6 parts, polyacrylic resin No. III 9 parts, 95% ethanol 150 parts, Pulvis Talci 2 parts, water for injection 20 parts.Wherein institute
The colony counts stating lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
Preparation method: (1) takes the lactic acid enterococcus faecalis of corresponding weight portion, starch, defatted milk by described Lactococcus micropill formula
Powder, microcrystalline Cellulose, dextrin, water for injection mix homogeneously, use extrusion spheronization machine to make micropill granule, and by this micropill granule
Fluid bed dryer is used to be dried below 40 DEG C to moisture less than 6%, standby；
(2) polyacrylic resin II of corresponding weight portion, polyacrylic resin are taken by described Lactococcus micropill formula
No. III, 95% ethanol, Pulvis Talci, soak after polyacrylic resin II and polyacrylic resin III are pulverized and be dissolved in
In 95% ethanol, it is subsequently adding Pulvis Talci, stirs, and use 80 mesh screen, take liquid standby；
(3) the micropill granule that step (1) prepares being added fluidized-bed coating machine, the solution using step (2) to prepare is carried out
Spray coating；
(4) after solution has been sprayed, continue to dry to moisture less than 5% by the micropill granule of coating, prepare described breast
Acid coccus micropill.
This Lactococcus micropill can generate pellet application directly as feedstuff, or is added into by a certain percentage
Normal diet uses.
Embodiment 3
This example energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, by following raw material system by weight
Become:
Lactic acid enterococcus faecalis 30 parts, starch 60 parts, defatted milk powder 8 parts, microcrystalline Cellulose 12 parts, 6 parts of dextrin, polyacrylic acid
Resin No. II 8 parts, polyacrylic resin No. III 8 parts, 95% ethanol 180 parts, Pulvis Talci 4 parts, water for injection 30 parts.Wherein institute
The colony counts stating lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
Its preparation method is same as in Example 1, and the Lactococcus micropill made also can generate granule directly as feedstuff and raise
Material application, or it is added in normal diet use by a certain percentage.
Embodiment 4
This example energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, by following raw material system by weight
Become:
Lactic acid enterococcus faecalis 50 parts, starch 50 parts, defatted milk powder 10 parts, microcrystalline Cellulose 10 parts, 4 parts of dextrin, polypropylene
Acid resin No. II 8 parts, polyacrylic resin No. III 7 parts, 95% ethanol 160 parts, Pulvis Talci 3 parts, water for injection 26 parts.Wherein
The colony counts of described lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
Its preparation method is same as in Example 1, and the Lactococcus micropill made also can generate granule directly as feedstuff and raise
Material application, or it is added in normal diet use by a certain percentage.
Embodiment 5
This example energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, by following raw material system by weight
Become:
Lactic acid enterococcus faecalis 10 parts, starch 70 parts, defatted milk powder 5 parts, microcrystalline Cellulose 6 parts, 8 parts of dextrin, polyacrylic acid
Resin No. II 10 parts, polyacrylic resin No. III 10 parts, 95% ethanol 200 parts, Pulvis Talci 4 parts, water for injection 40 parts.Wherein
The colony counts of described lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
Its preparation method is same as in Example 1, and the Lactococcus micropill made also can generate granule directly as feedstuff and raise
Material application, or it is added in normal diet use by a certain percentage.
The Lactococcus micropill that above-described embodiment 1-5 prepares is carried out respectively " dissolution in vitro test " as follows, " external adds
Heat test " and " actual Pelleting is on the impact test of lactic acid enterococcus faecalis viable count ":
One, dissolution in vitro Experimental Comparison
Test method: the Lactococcus micropill sample that Example 1-5 prepares, each portion of isodose, use paddle method, point
Sample does not carry out in 100 parts of normal saline, simulated gastric fluid, simulated intestinal fluids Dissolution Rate Testing, and detection different time difference is molten
Micropill in liquid observes change and viable count.Result is as shown in table 1.
Table 1 lactic acid enterococcus faecalis micropill dissolution determination result
Conclusion: by embodiment 1-5 1,2,3 hours outward appearance and liquid in simulated gastric fluid, normal saline and simulated intestinal fluid
Middle living stems illustrates: 1. this Lactococcus micropill is in simulated gastric fluid, 1,2,3 hours complete appearance of normal saline, has viable bacteria, can
Can bring into for micropill surface, within 1-3 hour, number of viable changes without significant difference, illustrates that micropill is in simulated gastric fluid and normal saline
Substantially not dissolution；2. micropill 1,2,3 hours outward appearances and number of viable change in simulated intestinal fluid can be seen that, microorganism in 1 hour
Dissolution is few, 1 hour to 2 hour, has a small amount of dissolution, and after 2 hours, dissolution is accelerated, 3 hours can substantially completely dissolution；3. micropill exists
Simulated gastric fluid and normal saline has pole significant difference with the dissolution in simulated intestinal fluid.4. the digestive system of animal is combined
And physiological metabolism feature, after micropill enters animal body, at the gastric of sour environment, coating of pellets layer is micro-in can effectively protecting ball
Biology is not released, and after entering small intestinal, along with enteral acid-base value raises, coating of pellets layer, by the most swelling decomposition, discharges ball
Interior microorganism, and food enters the intestinal time of 2-3 hour, food will be in the small intestinal second half section；Therefore, can be said by this test
This micropill bright can pinpoint and start release in small intestinal posterior segment, discharges completely after entering large intestine.
Two, external heat run contrast
Test method: the Lactococcus micropill sample that Example 1-5 prepares, each two parts of isodose, a test group,
A matched group；After test group sample wraps up with fine screen mesh, it is placed in 95 DEG C of hot baths, heats 3 minutes, take out afterwards, grind, use
Simulated intestinal fluid shakes 3 hours, detects number of viable；After control sample sampling, directly grind, shake 3 hours with simulated intestinal fluid
After, detect number of viable.Test group is as shown in table 2 with matched group number of viable result.
| Test group viable count (cfu/g) | Matched group viable count (cfu/g) | |
| Embodiment 1 | 1.13×1010 | 1.12×1010 |
| Embodiment 2 | 1.86×1010 | 1.94×1010 |
| Embodiment 3 | 1.48×1010 | 1.49×1010 |
| Embodiment 4 | 2.32×1010 | 2.37×1010 |
| Embodiment 5 | 6.12×109 | 5.9×109 |
Table 2 lactic acid enterococcus faecalis micropill heat run measurement result
Conclusion: test group sample viable count is compared with control sample viable count, and difference is the most notable, it was demonstrated that the present invention prepares
Lactic acid enterococcus faecalis be capable of withstanding 95 DEG C 3 minutes.
Three, actual Pelleting affects Experimental Comparison to lactic acid enterococcus faecalis viable count
Test method: the Lactococcus micropill sample that Example 1-5 prepares, each two parts of isodose, a test group,
A matched group；Test group is each added in the pellet formula of identical 10000 part respectively, according to identical pellet
The technological process of production produces, and takes the pellet containing corresponding lactic acid enterococcus faecalis micropill, grinds, shakes with simulated intestinal fluid
After 3 hours, detect number of viable；After control sample is according to 1000 parts of identical pellet formula mixing, the most expanded granulation,
Direct sample, grinds, and after shaking 3 hours with simulated intestinal fluid, detects number of viable.Test group is with matched group number of viable result such as
Shown in table 3.
| Test group viable count (cfu/g) | Matched group viable count (cfu/g) | |
| Embodiment 1 | 1.12×106 | 1.18×106 |
| Embodiment 2 | 1.97×106 | 1.89×106 |
| Embodiment 3 | 1.57×106 | 1.63×106 |
| Embodiment 4 | 2.34×106 | 2.41×106 |
| Embodiment 5 | 6.35×105 | 6.19×105 |
The actual Pelleting of table 3 affects test determination result to lactic acid enterococcus faecalis viable count
Conclusion: test group sample viable count is compared with control sample viable count, and difference is the most notable, it was demonstrated that the present invention prepares
Lactic acid enterococcus faecalis be capable of withstanding technique during particulate material feed manufacturing and do not destroy death.
Four, the lactic acid enterococcus faecalis that alternative embodiment 2 prepares carries out Piglet feed test, specific as follows:
Test pig farm: pig farm is planted by animal husbandry company of Sichuan cavalry；
Experimental animal: 23 days age in days Yorkshire ablactational baby pig；
Test method: choose identical age in days piglet 90, be randomly divided into three groups, is respectively test 1 group, tests 2 groups and right
According to group, test 1 group in normal feedstuff per ton, add this lactic acid enterococcus faecalis micropill 100g, test 2 groups at normal feedstuff per ton
Middle interpolation this lactic acid enterococcus faecalis micropill 200g, matched group, without, Direct-fed normal feedstuff, uses 25 days, recording process
And measurement data is as shown in table 4.
Table 4 Piglet feed test record and measurement data
Result of the test: testing 1 group of relatively matched group average weight gain and improve 5.4%, feedstuff-meat ratio reduces by 2.5%, test 2 groups more right
Improving 20.0% according to group average weight gain, feedstuff-meat ratio reduces by 14.6%, tests 1 group and test 2 groups reduces 42 times than matched group diarrhoea,
Diarrhea rate reduces by 5%；Visible, the lactic acid enterococcus faecalis micropill that the present invention prepares adds as feedstuff feeds piglet, it is possible to fixed point
In small intestinal back segment and big enteral release, improve the lactic acid enterococcus faecalis quantity of big intestinal segment, suppress Escherichia coli Growth, improve piglet
Digestive and absorptive functions and the minimizing disease of big intestinal segment occur, and therefore can improve piglet growth weight, reduce feedstuff-meat ratio, and significantly drop
Low piglet diarrhea rate, and show by result of the test, lactic acid enterococcus faecalis micropill is more excellent according to 200g/ ton additive effect.
Above-described embodiment is only the preferred embodiments of the present invention, not limiting the scope of the invention, as long as using
The design principle of the present invention, and carry out non-creativeness work on this basis and the change made, all should belong to the present invention's
Within protection domain.
Claims (5)
1. an energy is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, it is characterised in that by by weight as
Lower raw material is made:
Lactic acid enterococcus faecalis 10-50 part, starch 35-70 part, defatted milk powder 5-10 part, microcrystalline Cellulose 5-15 part, dextrin
3-8 part, polyacrylic resin II 5-15 part, polyacrylic resin III 5-15 part, 95% ethanol 100-200 part, Talcum
Powder 2-5 part, water for injection 20-60 part.
A kind of energy the most according to claim 1 is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, and its feature exists
In, it is made up of following raw material by weight:
Lactic acid enterococcus faecalis 20 parts, starch 65 parts, defatted milk powder 5 parts, microcrystalline Cellulose 10 parts, 5 parts of dextrin, polypropylene
Acid resin No. II 6 parts, polyacrylic resin No. III 9 parts, 95% ethanol 150 parts, Pulvis Talci 2 parts, water for injection 35 parts.
A kind of energy the most according to claim 1 is high temperature resistant and can pinpoint the Lactococcus micropill formula of release, and its feature exists
In, it is made up of following raw material by weight:
Lactic acid enterococcus faecalis 40 parts, starch 56 parts, defatted milk powder 8 parts, microcrystalline Cellulose 14 parts, 5 parts of dextrin, polypropylene
Acid resin No. II 6 parts, polyacrylic resin No. III 9 parts, 95% ethanol 150 parts, Pulvis Talci 2 parts, water for injection 20 parts.
4. high temperature resistant according to a kind of energy described in any one of claims 1 to 3 and can pinpoint release Lactococcus micropill formula,
It is characterized in that, the colony counts of described lactic acid enterococcus faecalis is not less than 100,000,000,000 cfu/ml.
5. a kind of energy as described in any one of Claims 1 to 4 is high temperature resistant and can pinpoint the Lactococcus micropill formula of release
Preparation method, it is characterised in that comprise the steps:
(1) the lactic acid enterococcus faecalis of corresponding weight portion, starch, defatted milk powder, crystallite fibre are taken by described Lactococcus micropill formula
Dimension element, dextrin, water for injection mix homogeneously, use extrusion spheronization machine to make micropill granule, and this micropill granule used fluidisation
Bed dryer is dried to moisture less than 6% below 40 DEG C, standby；
(2) polyacrylic resin II of corresponding weight portion, polyacrylic resin III are taken by described Lactococcus micropill formula
Number, 95% ethanol, Pulvis Talci, soak after polyacrylic resin II and polyacrylic resin III are pulverized and be dissolved in 95% wine
In essence, it is subsequently adding Pulvis Talci, stirs, and use 80 mesh screen, take liquid standby；
(3) the micropill granule that step (1) prepares being added fluidized-bed coating machine, the solution using step (2) to prepare is sprayed
Coating；
(4) after solution has been sprayed, continue to dry to moisture less than 5% by the micropill granule of coating, prepare described Lactococcus
Micropill.
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| CN110024905A (en) * | 2019-05-22 | 2019-07-19 | 河南聚推牧业有限公司 | A kind of enterococcus faecalis feed addictive |
| CN110241043A (en) * | 2019-06-10 | 2019-09-17 | 大连理工大学 | A bacterial strain producing lactic acid by high-temperature fermentation and a method for producing lactic acid |
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