CN106035382A - Bactericide composition containing dinitroaniline bactericides and biocontrol bacterium, preparation and application of bactericide composition and preparation - Google Patents
Bactericide composition containing dinitroaniline bactericides and biocontrol bacterium, preparation and application of bactericide composition and preparation Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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Abstract
The invention discloses a bactericide composition containing dinitroaniline bactericides and a biocontrol bacterium, a preparation and application of the bactericide composition and the preparation, and belongs to the field of plant protection and pesticide science. The bactericide composition comprises dinitroaniline bactericides and biocontrol bacterium powder, wherein the mass ratio of the dinitroaniline bactericides to the biocontrol bacterium powder is (1-10) to (1-10); the viable count of the bacterium powder is not lower than 10<6>cfu/g; the biocontrol bacterium is preferably bacillus amyloliquefaciens ZY-9-13, with collection number of CGMCC No.6162. The bactericide composition has the beneficial effects that fluazinam as the dinitroaniline bactericide can uncouple oxidation and phosphorylation in respiration of pathogenic bacteria, hinder energy synthesis and inhibit mycelial growth, sporogenesis and germination of the pathogenic bacteria; by using the bactericides and bacillus amyloliquefaciens cooperatively, the bactericide composition has excellent control effects on tobacco root knot nematode and tobacco black shank, has obviously better control effects than the singly used dinitroaniline bactericides or biocontrol bacterium bacillus amyloliquefaciens, can achieve the effect of delaying the drug resistance of chemical agents and is especially suitable for controlling tobacco black shank in the field.
Description
Technical field
The present invention relates to a kind of bacterium drug composition containing dinitroaniline series bactericidal agent and biocontrol microorganisms, also relate to bag
Preparation and application thereof containing this bacterium drug composition, belongs to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, its cause of disease
Bacterium is phytophthora oronoco [Phytophthora parasitica var nicotianae (Breda de Hann)
Tuker].Phytophthora nicotianae all can infect to Adult plant in the cigarette young plant phase, mainly infects tobacco stem stalk, it is possible to infect blade.Nicotiana tabacum L.
Balck shank forms water stain shape black speck, cortical tissue's blackening depression at stem foot, and extends up and down along stem, and marrow pitchy necrosis is also
Drying shrinkage be video disc shape (Wan Weixiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014
(02):50-50).Blade catch an illness after just for water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is wheel stricture of vagina at edge
Plaster shape black speck, Herb wilt (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. Hubei agriculture
Industry science, 2011,50 (16): 3253-3256).Phytophthora nicotianae with mycelium and akinete with diseased plant residuum at soil or heap
Fertilizer is survived the winter, becomes the primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged pass through surface flow
Water or with wind and rain infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. south agriculture
Industry journal, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, the black shin of Nicotiana tabacum L.
Sick extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J].
Chinese Tobacco Science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, wherein metalaxyl, dimethomorph, fluopicolide, fluorine pyridine
The pesticide such as amine are to use relatively broad medicament in production.Fluazinam (SHIRLAN, IKF-1216) is to be originated in industry public affairs by Japan's stone
The 2 of department's exploitation, 6-dinitroaniline wide-spectrum bactericide, infected with the resistance such as sprouting, mycelial growth by suppression Sporulation,
Resistance of rainwater washing against, lasting period are long, and the multiple gray mold causing Botrytis cinerea has specially good effect, same to benzimidazole, dicarboximide
Class and the most existing antibacterial no interactions resistance, to interlink spore genus, Phytophthora, Plasmopara, Sclerotinia and black
The germ diseases such as star Pseudomonas also have good activity.
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Biocontrol bacterial strain is utilized to prevent and treat cigarette
Grass balck shank has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
Plant the bacterial strain of preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196, this bacterium
Strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Notification number
The patent of invention of CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, and preservation is compiled
Number CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Notification number
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, entitled Xie Dian disclosed in the patent of invention of CN102925393B
Afnyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, root knot nematode in tobacco and balck shank are respectively provided with anti-by this bacterial strain
Controlling effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biocontrol effect is notable.But
It is that above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can
Alleviate the pressure to environment, delay to chemical agent produce resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical association
With preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and
Chemical agent is compounding all has good prevention effect to multiple pathogen.Semen Tritici aestivi can be prevented and treated as Tarlaromyces flavus and first frost MnZn compound
Head blight (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. biocontrol bacterial strain MT-06 fermentation condition and composite bactericide are to wheat scab
Preventive effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), compounding can to prevent and treat Semen Viciae fabae withered for Bacillus subtillis and Tebuconazole
(Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and potentiation for Chen Zhiyi, Haiying REN for disease of withering
Mechanisms [J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can
Prevent and treat ring rot of apple (week is cold. antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Anhui agriculture
Sparetime university is learned, 2009), jingganmycin and Bacillus subtillis collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. Jing Gang
Mycin and biocontrol microorganisms Bs-916 work in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.)
Etc., but the preventing and treating target that above bacterium recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A
Disclose a kind of bacterium drug composition for preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to matter
Amount is more compounding than 1.21～2.34:97.96～98.79 to be formed, and compares and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium medicine
Compositions can significantly improve the disease control effect such as black shank, rust, strengthens plant disease resistance, but also only reach with
The prevention effect that chemical agent is suitable.
Summary of the invention
It is an object of the invention to provide a kind of bacterium drug composition containing dinitroaniline series bactericidal agent and biocontrol microorganisms, this group
Compound has excellent prophylactic-therapeutic effect to tobacco root-knot, black shank, and preventive effect is significantly better than and uses single dinitro
Base aniline fungicides or biocontrol microorganisms microbial inoculum, it can delay the drug-fast generation of chemical agent.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffer a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or
Application in black shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, is made up of dinitroaniline series bactericidal agent and biocontrol microorganisms mycopowder, by quality ratio, and dinitroaniline
Series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Described dinitroaniline series bactericidal agent is selected from fluazinam etc..Preferably, dinitroaniline series bactericidal agent: biocontrol microorganisms bacterium
Powder=2～6:1.Antibacterial and biocontrol microorganisms are used in combination the prevention effect to tobacco root-knot, black shank etc. and are better than
It is used alone chemical bactericide or biocontrol microorganisms microbial inoculum.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Wherein, biocontrol microorganisms is excellent
Elect bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13 as, deposit number: CGMCC No.6162,
The preservation time: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Unit address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially can
Suppression tobacco black shank bacterium mycelial growth and spore germination, grow the most surely in tobacco rhizosphere.See patent " a kind of anti-root knot of holding concurrently
The Nicotiana tabacum L. endophyte of nematicide and Phytophthora nicotianae and application thereof " (notification number: CN102925393B).Experiment proves that, dinitro benzene
Amine antibacterial is faint to the growth effect of bacillus amyloliquefaciens (CGMCC No.6162), and the compatibility therebetween is good,
Bacterium medicine combination can be carried out.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettability
Powder, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..In preparation
Bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described help
Agent includes wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and other
It is of value to effective ingredient and the most stably plays the material of drug effect, be conventional in pesticidal preparations or the various one-tenth of permission use
Point.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, ten
Dialkyl benzene sulfonic acids calcium, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate, alkane
Base phenol polyethenoxy ether formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate, alkane
Base naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. any one
Plant or multiple.
Described stabilizer is selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol, Semen Ricini
Oil, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, tolyl shrink sweet
Any one or more in oil ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium, Gypsum Fibrosum, sulfur
Any one or more in acid sodium, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer,
C8～10Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethylformamide,
Any one or more in ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier be selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, bentonite,
Any one or more in Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc..
The application of bacterium drug composition, specially bacterium drug composition are in preventing and treating root knot nematode in tobacco and/or black shank
Application.
The application of bacterium medicine preparation, specially bacterium medicine preparation answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is by dinitroaniline series bactericidal agent and bacillus amyloliquefaciens (CGMCC No.6162)
Mycopowder forms, the oxidative phosphorylation uncoupling during wherein the fluazinam of dinitroaniline can make pathogen Repiration, hinders
Energy synthesizes, and the suppression mycelial growth of pathogen, Sporulation and sprouting, with bacillus amyloliquefaciens with the use of to Tobacco Root
Root-knot, black shank have the prophylactic-therapeutic effect of excellence, and preventive effect is significantly better than and uses single dinitroaniline sterilization
Agent or bacillus amyloliquefaciens biocontrol agent, and the drug-fast generation of chemical agent can be delayed, it is particularly well-suited to field tobacco black
The preventing and treating that shin is sick.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, is made up of fluazinam and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fluazinam: bacillus amyloliquefaciens mycopowder=10:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of fluazinam and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fluazinam: bacillus amyloliquefaciens mycopowder=1:10, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 3
Bacterium drug composition, is made up of fluazinam and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fluazinam: bacillus amyloliquefaciens mycopowder=1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 4
Bacterium drug composition, is made up of fluazinam and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fluazinam: bacillus amyloliquefaciens mycopowder=2:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 5
Bacterium drug composition, is made up of fluazinam and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with mass ratio
Meter, fluazinam: bacillus amyloliquefaciens mycopowder=6:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 6
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 25 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 15 parts6Cfu/g), dodecyl sodium sulfate 10 parts, Laurel alcohol radical sulphuric acid
10 parts of sodium, Kaolin 25 parts；Preparation method is: accurately takes each raw material, mix homogeneously according to mass fraction, obtains wettable powder
Agent.
Embodiment 7
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 45 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 8 parts, pulls open 3 parts of powder, forms sediment
4 parts of powder, 3 parts of sodium sulfate, xanthan gum 4 parts, sucrose 12 parts, bentonite 40 parts；Preparation method is: accurately take each according to mass fraction
Raw material, pelletize after mix homogeneously, it is dried, sieves, obtain suspending agent.
Embodiment 8
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 7 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 35 parts6Cfu/g), sodium lignin sulfonate 5 parts, sodium lignin sulfonate 4 parts,
Nekal 5 parts, dodecylbenzene sodium sulfonate 4 parts, polyvinylpyrrolidone 3 parts, starch 2 parts, sucrose 6 parts, diatom
20 parts of soil；Preparation method is: accurately takes each raw material, ultra micro comminution by gas stream after mix homogeneously, pelletize according to mass fraction, obtains water
Dispersible granule.
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 35 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 15 parts6Cfu/g), dodecyl sodium sulfate 6 parts, calcium lignosulfonate 8
Part, sucrose 8 parts, 20 parts of kieselguhr；Preparation method is with embodiment 6.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fluazinam 15 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 30 parts6Cfu/g), calcium lignosulfonate 5 parts, sodium lauryl sulphate 6
Part, sucrose 4 parts, white carbon 10 parts, 15 parts of kieselguhr；Preparation method is with embodiment 6.
In the various embodiments described above, bacillus amyloliquefaciens (CGMCC No.6162) mycopowder is with reference to patent (notification number
CN102925393B) in detailed description of the invention, method is prepared as wettable powder.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule,
Auxiliary agent uses this area conven-tional adjuvants.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and bacterium drug composition
Insecticide sensitivity.In process group, bactericide original drug (not adding bacillus amyloliquefaciens) is 1.00 μ g/mL for examination concentration, Xie Dian
The concentration of afnyloliquefaciens (CGMCC No.6162) bacteria suspension is 1010Cfu/mL, bacterium drug composition (bactericide original drug 1.00,
2.00,6.00,10.00 μ g/mL+ bacillus amyloliquefaciens (CGMCC No.6162) 1010Cfu/mL), compounding form see table 1.
Diameter 5mm bacterium dish is placed in the oat medium central authorities of pastille, and every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses
Decussation method measures colony diameter, calculates the different disposal suppression ratio to tobacco black shank bacterium, evaluates bacterium drug composition to black
The inhibitory action of shin pathogenic bacteria, result see table 1.
Use Abbott mathematical model calculate bacterium drug composition to the inhibition of pathogen (Colby S,
Calculating synergistic and antagonistic responses of herbicide combinations
[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent medicament A, medicine respectively
Agent B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as short of money
Anti-effect.
The suppression of tobacco black shank bacterium mycelial growth is imitated by table 1 dinitroaniline series bactericidal agent with bacillus amyloliquefaciens
Really
As it can be seen from table 1 compare chemical bactericide or bacillus amyloliquefaciens, the suppression of tobacco black shank bacterium is imitated
Really, bacterium drug composition shows synergistic function.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculating tobacco black shank bacterium by greenhouse, and spray bacterium medicine preparation prepared by embodiment 6～10, concrete grammar is such as
Under: the method with reference to Dong Yu prepares bacterium paddy inoculum, is added by the bacterium cake of 20 diameter 5mm in the millet of 500g sterilizing, 28 DEG C
Cultivation 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D]. the Chinese Academy of Agricultural Sciences,
2015)；Zhongyan-100 tobacco seedlings being planted in the nutritive cube of 10 × 10cm, Nutrition Soil is 1:1 with the ratio of Vermiculitum, treats that tobacco seedlings is long
During to 6～7 true leaves, carrying out medicament root irrigation, each nutritive cube adds medicinal liquid 50mL, and each process 5 strain tobacco seedlings, not apply
Medicament, add the process of 50mL clear water as comparison.Test sets three repetitions, inoculates bacterium paddy 10g/ in cigarette strain root after dispenser 3d
Strain, carries out disease survey after dispenser 7d, according to below equation calculating prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), test knot
Fruit see table 2.In table 2,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 500g/L fluazinam
Suspending agent is purchased from Ishihara Sangyo Kaisha, Ltd. of Japan, and bacillus amyloliquefaciens (CGMCC No.6162) microbial inoculum is with reference to patent (bulletin
Number CN102925393B) prepared by method in detailed description of the invention, is more than matched group.
The table 2 bacterium medicine preparation prevention effect to black shank
From table 2 it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 6～10,
Preventive effect reaches more than 75%, is better than commercially available chemical agent and bacillus amyloliquefaciens microbial inoculum, and the preventing and treating for balck shank provides newly
Type alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (8)
1. bacterium drug composition, it is characterised in that: it is made up of dinitroaniline series bactericidal agent and biocontrol microorganisms mycopowder, by quality ratio,
Dinitroaniline series bactericidal agent: biocontrol microorganisms mycopowder=1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described dinitroaniline series bactericidal agent is fluorine pyridine
Amine.
Bacterium drug composition the most according to claim 1 and 2, it is characterised in that: described biocontrol microorganisms is bacillus amyloliquefaciens
ZY-9-13, deposit number: CGMCC No.6162.
Bacterium drug composition the most according to claim 3, it is characterised in that: dinitroaniline series bactericidal agent: biocontrol microorganisms mycopowder
=2～6:1.
5. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of Claims 1 to 4.
Bacterium medicine preparation the most according to claim 5, it is characterised in that: also comprise in one or more Pesticide Sciences acceptable
Auxiliary agent.
7. as according to any one of Claims 1 to 4 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
8. bacterium medicine preparation application in preventing and treating root knot nematode in tobacco and/or black shank as described in claim 5 or 6.
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