CN106031348B - Simple method for rapidly detecting infection amount of ring rot germs in apple branches - Google Patents
Simple method for rapidly detecting infection amount of ring rot germs in apple branches Download PDFInfo
- Publication number
- CN106031348B CN106031348B CN201510110691.5A CN201510110691A CN106031348B CN 106031348 B CN106031348 B CN 106031348B CN 201510110691 A CN201510110691 A CN 201510110691A CN 106031348 B CN106031348 B CN 106031348B
- Authority
- CN
- China
- Prior art keywords
- branch
- branches
- paraquat
- lenticels
- apple
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 208000015181 infectious disease Diseases 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 10
- 244000052616 bacterial pathogen Species 0.000 title description 3
- 244000052769 pathogen Species 0.000 claims abstract description 23
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 20
- 238000001514 detection method Methods 0.000 claims abstract description 11
- 230000003902 lesion Effects 0.000 claims abstract description 9
- 229920000742 Cotton Polymers 0.000 claims abstract description 8
- 230000001338 necrotic effect Effects 0.000 claims abstract description 4
- 239000004744 fabric Substances 0.000 claims abstract description 3
- 230000002745 absorbent Effects 0.000 claims abstract 2
- 239000002250 absorbent Substances 0.000 claims abstract 2
- 239000002985 plastic film Substances 0.000 claims abstract 2
- 229920006255 plastic film Polymers 0.000 claims abstract 2
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 claims description 33
- 239000004009 herbicide Substances 0.000 claims description 7
- 230000002363 herbicidal effect Effects 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 5
- 238000011835 investigation Methods 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000034994 death Effects 0.000 claims description 4
- 239000002552 dosage form Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims 3
- 241000233866 Fungi Species 0.000 claims 2
- 241000220225 Malus Species 0.000 claims 2
- 241000700141 Rotifera Species 0.000 claims 2
- 239000004480 active ingredient Substances 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 210000003046 sporozoite Anatomy 0.000 claims 2
- 235000021016 apples Nutrition 0.000 claims 1
- 230000001524 infective effect Effects 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 3
- 206010064487 Oral mucosal exfoliation Diseases 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 244000141359 Malus pumila Species 0.000 description 19
- 235000011430 Malus pumila Nutrition 0.000 description 19
- 235000015103 Malus silvestris Nutrition 0.000 description 19
- 238000011081 inoculation Methods 0.000 description 17
- 206010039509 Scab Diseases 0.000 description 15
- 210000003491 skin Anatomy 0.000 description 15
- 239000012530 fluid Substances 0.000 description 14
- 238000012545 processing Methods 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 230000017074 necrotic cell death Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000190146 Botryosphaeria Species 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 4
- 235000010724 Wisteria floribunda Nutrition 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 230000003020 moisturizing effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000001054 cortical effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000005156 Dehydration Diseases 0.000 description 1
- 208000032420 Latent Infection Diseases 0.000 description 1
- 244000134242 Malus prunifolia Species 0.000 description 1
- 235000005087 Malus prunifolia Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 208000037771 disease arising from reactivation of latent virus Diseases 0.000 description 1
- 230000008641 drought stress Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000005081 epithelial layer Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000010129 solution processing Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000009333 weeding Methods 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了一套快速检测苹果枝条内轮纹病菌侵染量的简易方法，涉及植物体内病原菌侵染量的快速检测技术。本发明适用于检测基部直径不超过3厘米活体苹果枝条的带菌量。具体方法为在待检测枝条的基部环剥皮层，宽度2~10毫米；用有效成份含量60g/L~100g/L的百草枯药液浸泡吸水纸、脱脂棉、棉线或布条，取出沥干后，缠绕在环剥口处，用胶带或塑料膜包严；从处理后的第3天开始，检查记录处理枝条上发生病变和未发生病变的皮孔数量，以及坏死皮层上是否产生分生孢子器。本发明的优点在于：在1~2周内检测明确苹果枝条是否潜带轮纹病菌，以及潜带病原菌的数量；操作方法简便，检测结果可靠；解决了常规调查无法检测枝干内轮纹病菌侵染量的问题。
The present invention discloses a simple method for rapidly detecting the infection amount of the pathogen in apple branches, and relates to a rapid detection technology for the infection amount of pathogens in plants. The present invention is suitable for detecting the infection amount of living apple branches with a base diameter not exceeding 3 cm. The specific method is to ring-peel the cortex at the base of the branch to be detected, with a width of 2 to 10 mm; soak absorbent paper, absorbent cotton, cotton thread or cloth strips with an effective ingredient content of 60 g/L to 100 g/L, take out and drain, wrap them around the ring-peeling mouth, and wrap them tightly with tape or plastic film; starting from the third day after the treatment, check and record the number of lenticels with and without lesions on the treated branches, and whether conidiophores are produced on the necrotic cortex. The present invention has the advantages of: detecting and clarifying whether the apple branches are potentially carrying the pathogen within 1 to 2 weeks, and the number of potential pathogens; the operation method is simple, and the detection results are reliable; and the problem that conventional surveys cannot detect the infection amount of the pathogen in branches and trunks is solved.
Description
Technical field
The invention belongs to plant disease detection field, it is related to the Fast Detection Technique of pathogen infection amount in plant.
Background technique
Ring rot of apple is the important disease on apple, and mainly cause harm limb and fruit are the important diseases in Chinese apple
Evil, Bohai Rim and the aggrieved of ancient riverbed of the Yellow River producing region are particularly acute.2008,7, Shandong etc. apple main product is saved, 88 orchards
A situation arises that investigation shows the overall incidence of eyespot in apple limb up to 77.6% for Trunk canker.
Ring rot of apple is by Ascomycotina grape seat chamber bacteriumBotryosphaeria dothideaCaused by infecting, disease
Bacterium mainly infects branch from positions such as hole skin, stomata, wounds, forms sick tumor, saddle scab and dry rot scab.
Existing research is found, after infection of Botryosphaeria berengriana f. sp. piricola branch, only minority infects germ and infecting the sick tumor of current year induction, is drawn
Macroscopic symptom is played, the season of growth of most germs perhaps afterwards falls ill successively or hides for a long time in hole skin
In tissue, when branch is by drought stress or extremely weak branch, latent germ induces saddle scab or dry in a short time
Maize ear rot spot.
The branch that artificial infection test result is inoculated with before showing by the end of August, has a small amount of germ to induce Visual symptoms；August part
The branch being inoculated with later induces visible aobvious disease since next season of growth.
Due to infecting there are latent infection phenomenon for ring rot of apple, researcher is difficult to understand pathogen by inoculation test
The amount of infecting and medicament control efficiency, it is also difficult to the definite content of molds in tree body is understood, to constrain the prevalence of ring spot
It learns and Control Technology research and development.
Summary of the invention
Aiming at the problem that in the presence of ring rot of apple research and monitoring, the purpose of the present invention is to provide a set of quick inspections
Infection of Botryosphaeria berengriana f. sp. piricola amount simple and easy method in apple branch is surveyed, is ring rot of apple epidemiology and Study of Prevention Technology and disease
Monitoring provides a set of simple, quick detection technique and method.
The present invention uses following technical principle: the Target spot pathogen inside cortex has been invaded, in apple branch decline and death
During or by water stress after, mushroom out extension, and kill host cell, formed bronzing circle dry rot scab,
Cause macroscopic lesion.According to the quantity for occurring bronzing dry rot scab on branch, it can be determined that intrusion cortex inner wheel
The quantity of line germ.It hides in hole skin, there are no the Target spot pathogens inside intrusion cortex, can be bad after branch cortical necrosis
It extends rapidly, is bred using the nutrient growth that branch necrosis cortex provides, and generate the visible conidium of appearance in dead skin layer
Device, according to pycnidia can be generated on branch, it can be determined that whether have latent Target spot pathogen on branch.
The technical solution adopted by the invention is as follows: form band girdle mouth in the base portion band girdle epidermis for checking branch, with certain dense
Paraquat (herbicide) medical fluid of degree is coated with band girdle mouth, so that herbicide is penetrated into xylem by wound, and keep branch slowly withered.
In branch decline death process, the quantity of the bronzing dry rot scab formed at branch hole skin is checked, or check withered branch
On whether there is or not the formation of Target spot pathogen pycnidia, infer detection branch whether by infection of Botryosphaeria berengriana f. sp. piricola and pathogen according to this
The amount of infecting.
It is as follows specifically to apply embodiment:
(1) it chooses branch: choosing living body branch of the diameter of stem no more than 3 centimetres as processing branch；
(2) band girdle branch: in the lower section of detection branch section, away from least 5 centimetres of bifurcation, with pocket knife band girdle cortex, expose wood
Matter portion, 2 ~ 10 millimeters of width；
(3) it handles branch: being configured to the paraquat water that effective component content is 50g/L ~ 100g/L with herbicides paraquat
Solution；Blotting paper, absorbent cotton, cotton thread or cloth etc. is taken to impregnate 3 ~ 5 minutes in medical fluid, after taking-up drains, uniform winding or packet
It is rolled on band girdle mouth, with adhesive tape or plastic foil Bao Yan, prevents medical fluid from volatilizing；Handling diameter is more than that 0.5 centimetre of the common of branch has
Component content 100g/L medical fluid is imitated, the common effective component content that processing diameter is no more than 0.5 centimetre of branch is 60g/L medical fluid；
(4) investigation records: processing branch is slowly wilted withered in 1 ~ 2 week；Since treated the 3rd day, note is checked
The hole skin quantity of bronzing circle dry rot scab is formed on record wilting branch and the hole skin quantity of lesion does not occur, with marking pen mark
Note morbidity hole skin, until scab cannot count in flakes；The quantity or percentage of lesion hole skin, represent Target spot pathogen at this
The amount of infecting on branch or by the percentage for infecting hole skin；
(5) supplement survey: if not occurring the hole skin of lesion on branch, in branch, treated in 2 ~ 3 weeks, checks branch
The pycnidia for whether thering is Target spot pathogen to be formed on item；If handling the pycnidia for generating Target spot pathogen on branch, show
Branch is by infection of Botryosphaeria berengriana f. sp. piricola, but germ is deep into not yet in hole skin tissue.
Noticed Problems: the toxicity of paraquat is big, there is strong destruction to epidermal tissue, and when application must wear protecting hand
Set, and pay attention to drug safety；When handling branch with paraquat medical fluid, other positions or other crops of tree body cannot be polluted, are prevented
Only generate phytotoxicity；Apple branch is withered after being handled with paraquat, is unable to regrowth.
The present invention has the advantages that the whether latent belt wheel line of clear apple branch can be investigated in 1 ~ 3 week using this invention
Germ, and the latent quantity with pathogen, operating method is easy, and testing result is reliable；Used herbicide is that market is routinely sold
The various dosage forms and various contents sold are used for the paraquat medicament of weeding.
Application case one, influence of the paraquat aqueous solution of various concentration to branch: in early July, 2014, with every milliliter
Containing 105The conidial suspension of a Target spot pathogen is inoculated with the branch of 0.3 ~ 1.0cm centimetres of base diameter of current year raw Fuji apple
Item is inoculated with the moisturizing of rear enclosure polybag 24 hours.After inoculation 7 days, with the base portion of pocket knife band girdle inoculation branch, width is 2 ~ 10 millis
Rice；Preparing paraquat content with 200g/L paraquat aqua (commercial herbicide) is the aqueous solution of 20g/L, 6%, 10% and 20%, takes length
Degree is the bonnet cotton of about 10cm, in paraquat medicament impregnate 3 ~ after five minutes, taking-up drains, wind at the band girdle mouth of branch, use
Plastic foil Bao Yan, prevents medical fluid from volatilizing.The medical fluid of each concentration handles 10 branches, is control with clear water.The 5th after medical fluid processing
It, check the blade, the cortex that occur obvious wilting symptom on branch there is the branch number of withered spot and withered spot area account for it is whole
The percentage of a branch area.
Test result: concentration is more than the branch of the paraquat aqueous solution processing of 60g/L, the blade the 3rd day after application
There is apparent wilting symptom, the necrosis cortex since the 4th day, survey data is as shown in table 1 within the 5th day.Survey data table
Bright, concentration is more than the branch of 60g/L paraquat medical fluid processing, and the 5th day most blades are wilted withered after chemicals treatment, is generated
Obvious necrotic plaque；The branch handled with the paraquat medical fluid of 20g/L does not have bright without the apparent variation of discovery after 5 days；In experiment also
It was found that the paraquat solution of 200g/L damages death that is very big, or even can lead to whole tree due to concentration height, to whole strain apple tree,
It is not recommended that using in factual survey；Suggest using concentration 60g/L ~ 100g/L in factual survey.
Table 1: after apple annotinous branch (10 branches) is handled with various concentration paraquat medical fluid the 5th day blade wilt and
Branch cortical necrosis situation.
Application case two: the recall rate of pathogen after inoculation different time: in by the end of July, 2014, contains 10 with every milliliter5A wheel
Branch of the conidial suspension inoculation diameter of line germ in the current year raw Fuji apple of 0.3 ~ 1.0cm, sleeving plastic after inoculation
Bag moisturizing 24 hours；By be inoculated with branch label after 4 groups of average mark, respectively after inoculation 7 days, 14 days, 21 days and 28 days it is laggard
Row band girdle, and handled with paraquat, 24 branches of each time-triggered protocol.The inspection record since treated the 5th day for paraquat
There is downright bad corticating branch item number and has the branch number of Target spot pathogen pycnidia.
Test result: in all inoculation branches after inoculation 10 days, necrotic plaque is all produced on cortex；Downright bad cortex from
Initially form pycnidia within 7th day, all inoculation branch all produced pycnidia by the 21st day, the inspection carried disease germs to branch
Extracting rate reaches 100%, and survey data is shown in Table 2.
Table 2: with Target spot pathogen conidium be inoculated with apple branch, cultivate different time after handled with paraquat after branch
Epithelial layer necrosis and the percentage for generating pycnidia.
Application case three gos deep into the detection of Target spot pathogen in cortex: in early August, 2013, contains 10 with every milliliter5A wheel line
The branch of the conidial suspension inoculation current year raw Fuji apple of germ, is inoculated with the moisturizing of rear enclosure polybag 24 hours.2014 5
The middle of the month is configured to effectively with the epidermis of pocket knife band girdle inoculation branch base portion with 200g/L paraquat aqua (commercial herbicide)
Component content is 100g/L medical fluid；Absorbent cotton to be impregnated 5 minutes in medical fluid, taking-up drains, equably it is wrapped at band girdle mouth,
And with preservative film Bao Yan.
Test result: after inoculation branch is handled with paraquat, since the 3rd day, a large amount of diameters 1 are formed centered on hole skin
~ 2 millimeters of bronzing circle dry rot scab.Dry rot scab is centered on hole skin, bronzing, and round, the necrosis with cortex has bright
Aobvious difference.Part Spot expansion is more than 5 millimeters after processing 5 days, and is started in flakes；Most of germ, which links, after processing 10 days is
It is a piece of；Black dot, i.e. the life spore device of Target spot pathogen are generated after processing 15 days under scab epidermis.
Detailed description of the invention: Fig. 1 is that formation 1 ~ 2mm of diameter is reddish brown centered on hole skin after paraquat is handled 3 days for inoculation branch
Color dry rot scab；Fig. 2 is that inoculation branch constantly extends after paraquat is handled 5 days, and starts scab in flakes；Fig. 3 is inoculation
Scab in flakes after branch is handled 10 days with paraquat；Fig. 4 is that inoculation branch scab after paraquat is handled 15 days is formed
Pycnidia.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510110691.5A CN106031348B (en) | 2015-03-13 | 2015-03-13 | Simple method for rapidly detecting infection amount of ring rot germs in apple branches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510110691.5A CN106031348B (en) | 2015-03-13 | 2015-03-13 | Simple method for rapidly detecting infection amount of ring rot germs in apple branches |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106031348A CN106031348A (en) | 2016-10-19 |
| CN106031348B true CN106031348B (en) | 2019-01-29 |
Family
ID=57151179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510110691.5A Expired - Fee Related CN106031348B (en) | 2015-03-13 | 2015-03-13 | Simple method for rapidly detecting infection amount of ring rot germs in apple branches |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106031348B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108410946A (en) * | 2018-03-20 | 2018-08-17 | 海南大学 | A method of it is pathogenic to measure cause of disease domestomycetes in basidiomycetes using live standing tree stem |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060292646A1 (en) * | 2002-11-26 | 2006-12-28 | Colpas Gerard J | Methods, biosensors, and kits for detecting and identifying fungi |
| CN101020687A (en) * | 2007-01-12 | 2007-08-22 | 南开大学 | New thiobizole formanide derivative and its synthesis process and bioactivity |
| CN101864481A (en) * | 2009-09-29 | 2010-10-20 | 青岛农业大学 | SCAR Molecular Marker Method for Detecting Apple Branch Ringworm Resistance Gene |
-
2015
- 2015-03-13 CN CN201510110691.5A patent/CN106031348B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060292646A1 (en) * | 2002-11-26 | 2006-12-28 | Colpas Gerard J | Methods, biosensors, and kits for detecting and identifying fungi |
| CN101020687A (en) * | 2007-01-12 | 2007-08-22 | 南开大学 | New thiobizole formanide derivative and its synthesis process and bioactivity |
| CN101864481A (en) * | 2009-09-29 | 2010-10-20 | 青岛农业大学 | SCAR Molecular Marker Method for Detecting Apple Branch Ringworm Resistance Gene |
Non-Patent Citations (3)
| Title |
|---|
| 关于果树营养与施肥的几个问题;刘成先;《北方果树》;20090630(第3期);46-48 * |
| 我国苹果主要病害研究进展与病害防治中的问题;李保华等;《植物保护》;20131031;第39卷(第5期);46-54 * |
| 招远市苹果主要病害发生情况调查报告;杨聚德等;《落叶果树》;20080630(第3期);31-33 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106031348A (en) | 2016-10-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101555458B (en) | A kind of Burkholderia pyrrole and its application in the prevention and treatment of poplar canker | |
| Chukwuka et al. | Microbial ecology of organisms causing pawpaw (Carica papaya L.) fruit decay in Oyo State, Nigeria | |
| CN102578170A (en) | Chinese herbal medicinal bactericide | |
| Raghuwanshi et al. | Characterization of Xanthomonas axonopodis pv. punicae isolates from western Maharashtra and their sensitivity to chemical treatments | |
| CN103766409A (en) | Walnut green seedcase extract product and bacteriostatic use thereof | |
| Arora | Eco-friendly management of soil and tuber borne diseases of potato | |
| CN106031348B (en) | Simple method for rapidly detecting infection amount of ring rot germs in apple branches | |
| CN107232238B (en) | Application of Litsea Cubeba Essential Oil in Controlling Pepper Blight | |
| Stewart | The fire blight disease in nursery stock | |
| CN110037028B (en) | Application of 6-pentyl-2H-pyran-2-one compound in preparation of pathogenic bacteria agent for resisting banana wilt and rice false smut | |
| Rahmadhani et al. | Growth responses of oil palm seedling inoculated with Ganoderma boninense under competition with edible herbaceous plants | |
| Epstein et al. | Crown gall can spread between walnut trees in nurseries and reduce future yields | |
| CN109362876A (en) | A kind of dragon fruit traditional Chinese medicine composite coating preservative and its preparation method and application | |
| Anderson | Diseases of Illinois fruits | |
| Katoch et al. | Perpetuation of Stemphylium blight of garlic under mid hill conditions of Himachal Pradesh | |
| Warakagoda et al. | In vitro seed germination of Coscinium fenestratum (Gaertn.) Colebr | |
| Rawat et al. | Major Diseases of Garlic (Allium Sativum L.) and Their Management | |
| CN105613605B (en) | Botanical aphicide locust seed insecticidal plant material | |
| Jawadagi et al. | Vegetable Science Frontiers: Insights of Olericulture | |
| CN103141350B (en) | Method for controlling apple fruit rust | |
| Misra | Diseases of guava | |
| JP3591250B2 (en) | Tide-resistant plant and method for producing the same | |
| van den Brink et al. | Rhizoctonia solani in potatoes and its control: Specific recommendations for seed production in Punjab (India) | |
| CN107372526A (en) | A kind of Difenoconazole liniment and its preparation method and application | |
| Saleeon et al. | GREEN TOBACCO SICKNESS AND PROTECTIVE BEHAVIORSAMONG THAI TRADITIONAL TOBACCO FARMERS IN NORTHERN THAILAND |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190129 |