CN105994943A - Animal feed attractant and preparation method thereof - Google Patents
Animal feed attractant and preparation method thereof Download PDFInfo
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- CN105994943A CN105994943A CN201610373654.8A CN201610373654A CN105994943A CN 105994943 A CN105994943 A CN 105994943A CN 201610373654 A CN201610373654 A CN 201610373654A CN 105994943 A CN105994943 A CN 105994943A
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- 241001465754 Metazoa Species 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000005667 attractant Substances 0.000 title abstract 5
- 230000031902 chemoattractant activity Effects 0.000 title abstract 5
- 238000000855 fermentation Methods 0.000 claims abstract description 30
- 230000004151 fermentation Effects 0.000 claims abstract description 30
- 230000001954 sterilising effect Effects 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 7
- 150000007524 organic acids Chemical class 0.000 claims abstract description 7
- 150000001491 aromatic compounds Chemical class 0.000 claims abstract description 6
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims abstract description 6
- 235000013923 monosodium glutamate Nutrition 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 229940073490 sodium glutamate Drugs 0.000 claims abstract description 6
- 229940024606 amino acid Drugs 0.000 claims abstract description 5
- 150000001413 amino acids Chemical class 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 5
- PVBRXXAAPNGWGE-LGVAUZIVSA-L disodium 5'-guanylate Chemical compound [Na+].[Na+].C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H]1O PVBRXXAAPNGWGE-LGVAUZIVSA-L 0.000 claims abstract description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 235000013902 inosinic acid Nutrition 0.000 claims description 9
- 244000063299 Bacillus subtilis Species 0.000 claims description 8
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 7
- 241000186145 Corynebacterium ammoniagenes Species 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 6
- 241000186226 Corynebacterium glutamicum Species 0.000 claims description 5
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 5
- GRSZFWQUAKGDAV-KQYNXXCUSA-N IMP Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-KQYNXXCUSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 235000013922 glutamic acid Nutrition 0.000 claims description 5
- 239000004220 glutamic acid Substances 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- XXLFLUJXWKXUGS-UHFFFAOYSA-N 6-methoxyquinoline-4-carboxylic acid Chemical compound N1=CC=C(C(O)=O)C2=CC(OC)=CC=C21 XXLFLUJXWKXUGS-UHFFFAOYSA-N 0.000 claims description 4
- 235000001014 amino acid Nutrition 0.000 claims description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 4
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 239000001630 malic acid Substances 0.000 claims description 2
- 235000011090 malic acid Nutrition 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 1
- 235000015165 citric acid Nutrition 0.000 claims 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 229940099596 manganese sulfate Drugs 0.000 claims 1
- 235000007079 manganese sulphate Nutrition 0.000 claims 1
- 239000011702 manganese sulphate Substances 0.000 claims 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims 1
- 239000011591 potassium Substances 0.000 claims 1
- 229910052700 potassium Inorganic materials 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 11
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000003674 animal food additive Substances 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- SLIUELDPAPMQIB-IDIVVRGQSA-N [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C=NC=2C(O)=NC=NC12 Chemical compound [Na].[Na].[C@@H]1([C@H](O)[C@H](O)[C@@H](COP(=O)(O)O)O1)N1C=NC=2C(O)=NC=NC12 SLIUELDPAPMQIB-IDIVVRGQSA-N 0.000 abstract 1
- 230000036528 appetite Effects 0.000 abstract 1
- 235000019789 appetite Nutrition 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 235000013896 disodium guanylate Nutrition 0.000 abstract 1
- 239000004198 disodium guanylate Substances 0.000 abstract 1
- 235000013890 disodium inosinate Nutrition 0.000 abstract 1
- 239000003925 fat Substances 0.000 abstract 1
- 235000005985 organic acids Nutrition 0.000 abstract 1
- 239000006072 paste Substances 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 10
- 239000000654 additive Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 230000000996 additive effect Effects 0.000 description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 4
- 230000037406 food intake Effects 0.000 description 3
- 235000012631 food intake Nutrition 0.000 description 3
- 235000019629 palatability Nutrition 0.000 description 3
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- KNLQKHUBPCXPQD-UHFFFAOYSA-N manganese;sulfuric acid Chemical compound [Mn].OS(O)(=O)=O KNLQKHUBPCXPQD-UHFFFAOYSA-N 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 108010011619 6-Phytase Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 235000019752 Wheat Middilings Nutrition 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- -1 compound vitamin Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013360 fish flour Nutrition 0.000 description 1
- 235000013928 guanylic acid Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 229940085127 phytase Drugs 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 238000013337 sub-cultivation Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
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- Fodder In General (AREA)
Abstract
The present invention belongs to the fields of microorganisms, fermentation and feed additives, and particularly relates to an animal feed attractant and a preparation method thereof. The attractant consists of the following components in weight percentages: 10%-50% of sodium glutamate, 10%-30% of disodium 5'-inosinate, 0.5%-5% of disodium guanylate, 2%-10% of amino acids, 2%-10% of organic acids, 0.5%-2% of fats and 0.5%-3% of aromatic compounds, and the sum of the weight percentage of each substance is 100%. The invention also provides a preparation method of the animal feed attractant. The method comprises the following steps: microorganism inoculating in a fermentation medium, seed culturing, fermenting, sterilizing, and concentrating/concentrating and drying. The obtained attractant can be liquid, paste or powder, and widely used in feed production process, and have food attracting and appetite promoting effects for animals and pets.
Description
Technical field
The invention belongs to microorganism, fermentation and feed additive field, particularly to a kind of animal
Feed eating belt agent and preparation method thereof.
Background method
The bad smell produced in conventional feed production process, easily affects animal palatability, from
And affect the food-intake of animal.For improving the food-intake of animal, people are often in conventional feed
Increase additive, though but these additives have the effect of delicate flavour, but nutritive value is little, has
A little additives also can precipitate toxin in animal bodies, and these toxin are final by food chain structure
Transfer to, on human body, affect health.
Summary of the invention
For above technical problem, the invention provides that one is of high nutritive value, food calling effect is strong,
And the animal feed additive having no side effect, add additive to animal is daily raises by a certain amount of
In material, feedstuff palatability can be improved, increase food-intake, promote that animal is mesotrophic to feedstuff
Absorb.
In order to solve above technical problem, the present invention adopts the following technical scheme that a kind of animal is raised
Material phagostimulant, its weight percent consists of: sodium glutamate 10%-50%, inosine monophosphate
10%-30%, guanosine monophosphate disodium 0.5%-5%, aminoacid 2%-10%, organic acid 2%-10%, fat
Fat 0.5%-2% and aromatic compounds 0.5%-3%, the weight percent composition sum of each material
It is 100%.Described aromatic compounds be those skilled in the art commonly use tannin, pigment
And aromatics.
Further: in above-mentioned animal feed phagostimulant, described organic acid is malic acid, lemon
At least one in lemon acid, lactic acid, quininic acid and acetic acid.
The method that present invention also offers above-mentioned animal feed phagostimulant, it includes inoculating strain
Cultivating seeds, fermentation, sterilizing, the process of concentration/concentrate drying is carried out in fermentation medium.
Described strain is at least one in glutamic acid bacillus, bacillus subtilis or corynebacterium ammoniagenes.
Described fermentating controling condition is, inoculum concentration is 10 ± 1%, and temperature is 30-37 DEG C, and PH is
6.6-7.2, tank pressure is 0.05MPa, dissolved oxygen >=15%.Described fermentation medium percentage by weight
Concentration proportioning be Semen Maydis starch 1.5-2%, sugared close 5-10%, potassium dihydrogen phosphate 0.1-0.3%,
Ammonium sulfate 0.2-0.5%, magnesium sulfate 0.1-0.3%, ferrous sulfate 0.03-0.05%, sulphuric acid
Manganese 0.03-0.05%, glucose 5-10%, surplus is water.Described concentration/concentrate drying is
Refer to concentrate the 3-8 times of creamy product obtained through negative pressure 50-90 DEG C after fermentation liquid sterilizing;Or again
Continue to be dried to obtain powder product by creamy product.Described fermentation refers to that corynebacterium ammoniagenes is fermented extremely
The concentration of inosine monophosphate IMP reaches 3%-12.0%;Or Corynebacterium glutamicum/bacillus subtilis
Bacterium fermentation reaches 5%-17.0% to fermentation liquid Glutamic Acid concentration;Or the two mixture.
In prepared by above-mentioned feedstuff food calling additive, the fermentation culture of described corynebacterium ammoniagenes
Temperature is in temperature 30-37 DEG C, and the time of fermentation culture is 60-100h;Corynebacterium glutamicum or
Person's fermentation of bacillus subtilis cultivation temperature is 32-41 DEG C;Two kinds of pure-blood ferment liquid mix in proportion
After conjunction, continue fermentation to fermentation liquid glucose content close to 0.Product ammonia bar used by the present invention
Bacterium, Corynebacterium glutamicum or bacillus subtilis are that art technology is approved also in food scope
Known strain.
Beneficial effect: compared with prior art, the present invention prepares feedstuff food calling additive technology tool
There is a following advantage:
(1) the glutamic acid bar that the feedstuff food calling additive cause nature of the present invention is widely present
Bacterium, corynebacterium ammoniagenes, bacillus subtilis process, and production technology is simple, are a kind of green
Color, eco-friendly feed additive;
Product the most of the present invention contains abundant aminoacid, organic acid, nucleotide and aromatic compound
Thing, and there is the fresh effect of increasing, animal is had obvious food calling effect;
Product form the most of the present invention can be emulsus, paste, bulk, powdery etc., conveniently makes an addition to
Follow-up feed production technology.
Even sodium glutamate and IMP or GMP the used time, it is possible to cover the abnormal flavour in feedstuff, change
Kind feedstuff palatability, promotes that feed digestion absorbs, increases yield, almost to all of fowl
Domestic animal, Fish, shrimps, shellfish all have the strongest food calling effect.In addition, nucleotide exists
Animal body normal immunological function aspects is maintained to play an important role.First, suitable in feedstuff
When adding nucleotide, the excrement bifidobacteria in animal intestinal can be made to preponderate, thus suppress
The propagation of enteropahtogenic microganism;Secondly, the growth of intestinal and maturation need extraneous nucleotide
Supply, makes enterocyte preferably breed, breaks up and maintain integrity.
Detailed description of the invention
Prepared by 1 seed liquor
Preparation Corynebacterium glutamicum, corynebacterium ammoniagenes/bacillus subtilis slant medium, training
Supporting basigamy ratio is: yeast extract 0.5%, glucose 2%, potassium dihydrogen phosphate 0.1%, sulphuric acid
Ammonium 0.2%, magnesium sulfate 0.1%, agar powder 1.5%;Strain is inoculated in slant medium,
Cultivate 24h for 37 DEG C, with sterilized water, the lawn on inclined-plane is washed till sterilized three and solves in bottle, system
Obtain bacteria suspension.
The preparation of 2 secondary seeds
Preparation secondary seed medium, proportioning is Semen Maydis pulp 0.5%, yeast extract 0.3%,
Potassium dihydrogen phosphate 0.1%, ammonium sulfate 0.1%, magnesium sulfate 0.1%, grape sugar sugar 2%;After dissolving
Enter fermentation tank constant volume, 125 DEG C of sterilizing 20min, be cooled to 37 DEG C and access seed bacteria suspension, send out
Ferment gets final product subcultivation when being 0.3-0.5 to OD net added value;
3, prepared by feedstuff food calling additive mixed liquor
Fermentation base Semen Maydis starch 1.5-2%, sugared close 5-10%, potassium dihydrogen phosphate 0.1-0.3%,
Ammonium sulfate 0.2-0.5%, magnesium sulfate 0.1-0.3%, ferrous sulfate 0.03-0.05%, sulphuric acid
Manganese 0.03-0.05%, glucose 5-10%, surplus is water.Wherein molasses, glucose are by one
Determine to divide after volume dissolves to disappear, after sterilization, pump into fermentation tank by fed-batch mode;Medium sterilization disappears
Adjusting PH to 6-6.7 with ammonia after poison cooling, secondary seed solution is accessed by 10% volume, maintains and sends out
Ferment tank pressure 0.05Mpa, tank temperature 30-37 DEG C, DO >=20%, by fermentation time and fermentation condition
Control fermentation termination to fermentation liquid sodium glutamate content be 10-20% or IMP content be 6-9%
Time, the sterilizing both are mixed in proportion obtain mixed liquor of can heating, mixed liquor is concentrated to
After certain viscosity, it is spray-dried, obtains the animal feed phagostimulant of the present invention.
Preferably constituting as sodium glutamate 40%-48%, inosine monophosphate of its phagostimulant
25%-30%, guanosine monophosphate disodium 3.5%-5%, aminoacid 8%-10%, organic acid 5%-7%, fat
Fat 0.5%-2% and aromatic compounds 1%-3%, the weight percent composition sum of each material is
100%.
4 products application
Product of the present invention is added to containing bean cake, wheat bran, cottonseed meal, Semen Maydis fibre with 8-15% ratio
Animal is thrown something and fed by the fermented feed of dimension, Testa oryzae, powdered rice hulls etc..
Test example 1
Fructus Hordei Vulgaris wheat-middlings 300 parts, Semen Maydis powder 200 parts, bean dreg powder 200 parts, 180 parts of Testa oryzae,
Fish flour 20 parts, lysine 2 parts, phytase 3 parts, cellulose close to obtain enzyme 5 parts, biological work
Property bacterium 5 parts, compound vitamin 8 parts, Sal 6 parts, antifoggant 3 parts, product of the present invention
10 parts, as testing the impact that live pig is digested and assimilated by product of the present invention, choose 50 ± 3kg
Live pig 100, is randomly divided into two groups, and matched group is without product of the present invention, and other raises bar
Part is identical, raises contrast effect and see table:
Experimental group (g) | Matched group (g) | |
Daily gain | 825±12 | 738±25 |
Feed to delivering day for sale | 53±2 | 59±3 |
Test 2
Test uses 96 child care piglets (original body mass 12.00 ± 0.5kg), according to piglet
The sow parity in source is close, the principle that male and female ratio is consistent, is assigned randomly to 4 groups, often
Individual group 4 repetitions, 6 piglets of each repetition, share 16 circles.Piglet enters nursery house
After by equal amount of mixture mixed feeding 3 days step by step of the former child care material fed and 4 kinds of testing materials;
Starting formal test afterwards, feed 4 kinds of daily rations respectively, experimental period is 4 weeks, and result of the test is shown in
Following table, as can be known from the results, feed eating belt agent of the present invention has obvious effect to the weightening finish of piglet food calling.
Claims (8)
1. an animal feed phagostimulant, its weight percent consists of: sodium glutamate 10%-50%,
Inosine monophosphate 10%-30%, guanosine monophosphate disodium 0.5%-5%, aminoacid 2%-10%, organic acid
2%-10%, fat 0.5%-2% and aromatic compounds 0.5%-3%, the weight hundred of each material
Being grouped into sum is 100%.
Animal feed phagostimulant the most according to claim 1, it is characterised in that: described
Organic acid be at least one in malic acid, citric acid, lactic acid, quininic acid and acetic acid.
3. the method preparing animal feed phagostimulant described in claim 1 or 2, its
It is characterised by: it includes being inoculated in strain fermentation medium and carries out cultivating seeds, ferments, goes out
Bacterium, the process of concentration/concentrate drying.
Preparation method the most according to claim 3, it is characterised in that: described strain is
At least one in glutamic acid bacillus, bacillus subtilis or corynebacterium ammoniagenes.
Preparation method the most according to claim 4, it is characterised in that: described fermentation
Control condition is, inoculum concentration is 10 ± 1%, and temperature is 30-37 DEG C, and PH is 6.6-7.2, tank
Pressure is 0.05MPa, dissolved oxygen >=15%.
Preparation method the most according to claim 3, it is characterised in that: described fermentation training
The concentration proportioning supporting base percentage by weight is Semen Maydis starch 1.5-2%, sugared close 5-10%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.1-0.3%, ammonium sulfate 0.2-0.5%, magnesium sulfate 0.1-0.3%, ferrous sulfate
0.03-0.05%, manganese sulfate 0.03-0.05%, glucose 5-10%, surplus is water.
Preparation method the most according to claim 3, it is characterised in that: described concentration/
Concentrate drying refers to concentrate the 3-8 times of paste obtained through negative pressure 50-90 DEG C after fermentation liquid sterilizing
Product;Or continue to be dried to obtain powder product by creamy product again.
Preparation method the most according to claim 3, it is characterised in that: described fermentation
Refer to that corynebacterium ammoniagenes fermentation reaches 3%-12.0% to the concentration of inosine monophosphate IMP;
Or Corynebacterium glutamicum/fermentation of bacillus subtilis reaches to fermentation liquid Glutamic Acid concentration
5%-17.0%;
Or the two mixture.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
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CN113632887A (en) * | 2021-06-07 | 2021-11-12 | 西南大学 | Preparation method and application of pig feeding accelerant |
CN113678951A (en) * | 2021-09-01 | 2021-11-23 | 齐齐哈尔龙江阜丰生物科技有限公司 | Preparation process of novel piglet food calling feed additive |
CN116998599A (en) * | 2023-08-16 | 2023-11-07 | 南京高新工大生物技术研究院有限公司 | An animal feed attractant derived from fungal extracts and its preparation method and application |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107467370A (en) * | 2017-10-13 | 2017-12-15 | 无锡正大生物股份有限公司 | A kind of feed feature phagostimulant and preparation method thereof |
CN113632887A (en) * | 2021-06-07 | 2021-11-12 | 西南大学 | Preparation method and application of pig feeding accelerant |
CN113678951A (en) * | 2021-09-01 | 2021-11-23 | 齐齐哈尔龙江阜丰生物科技有限公司 | Preparation process of novel piglet food calling feed additive |
CN116998599A (en) * | 2023-08-16 | 2023-11-07 | 南京高新工大生物技术研究院有限公司 | An animal feed attractant derived from fungal extracts and its preparation method and application |
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