CN105986324B - 环状小rna文库构建方法及其应用 - Google Patents
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Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
cDNA | 10ul |
上游引物 | 1ul |
10N pfN缓冲液 | 2ul |
50mM硫酸镁 | 0.4ul |
10mM dNTP | 0.6ul |
pfN | 0.4ul |
水 | 4.6ul |
total | 20ul |
样品名 | 总reads数 | 过滤后reads数 | 接头自连数目 | 接头自连比例 |
All | 291080193 | 232764154 | 3314488 | 1.14% |
UHRR | 35248461 | 28168769 | 622612 | 1.77% |
UHRR | 36087532 | 28670026 | 355512 | 0.99% |
HBRR | 37059530 | 29747624 | 417809 | 1.13% |
HBRR | 36878800 | 29703040 | 324104 | 0.88% |
小鼠 | 32678723 | 26182978 | 558211 | 1.71% |
小鼠 | 39329268 | 31453414 | 326436 | 0.83% |
水稻 | 38900191 | 31130153 | 362346 | 0.93% |
水稻 | 34897688 | 27818150 | 347458 | 1.00% |
样品名 | 总reads数 | 过滤后reads数 | 接头自连数目 | 接头自连比例 |
All | 345821023 | 325071762 | 205697807 | 63.28% |
UHRR1 | 110780011 | 104133210 | 72749778 | 69.86% |
UHRR2 | 98984241 | 93045187 | 60746735 | 65.29% |
HBRR1 | 60965348 | 57307427 | 33183607 | 57.90% |
HBRR2 | 75091423 | 70585938 | 39017687 | 55.28% |
Claims (14)
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CN105986324B true CN105986324B (zh) | 2018-08-14 |
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Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108070642B (zh) * | 2016-11-17 | 2020-10-27 | 深圳华大智造科技股份有限公司 | 提高dnb双末端测序质量的方法和dnb双末端测序方法和试剂盒 |
CN108203847B (zh) * | 2016-12-16 | 2022-01-04 | 深圳华大智造科技股份有限公司 | 用于二代测序质量评估的文库、试剂及应用 |
CN115927566B (zh) * | 2017-09-20 | 2025-02-18 | 深圳华大智造科技股份有限公司 | 一种用于Small RNA的测序方法、测序试剂和应用 |
CN108060460A (zh) * | 2018-01-17 | 2018-05-22 | 深圳华大生命科学研究院 | 一种small RNA的文库构建及测序方法 |
US20210024933A1 (en) * | 2018-04-28 | 2021-01-28 | Bgi Genomics Co., Ltd. | Splint nucleic acid molecule for cyclizing single-stranded nucleic acid molecule and use thereof |
CN110872609B (zh) * | 2018-09-04 | 2024-06-04 | 深圳华大基因科技服务有限公司 | 对小rna分子精准建库和测序的方法以及应用 |
US20220033809A1 (en) * | 2018-09-05 | 2022-02-03 | Bgi Shenzhen | Method and kit for construction of rna library |
US20220177964A1 (en) * | 2019-03-27 | 2022-06-09 | Diagenode S.A. | A high throughput sequencing method and kit |
CN111909991B (zh) * | 2019-05-09 | 2021-08-03 | 中国科学院生物物理研究所 | 一种捕获rna原位高级结构及相互作用的方法 |
CN119685448A (zh) * | 2019-05-15 | 2025-03-25 | 深圳华大生命科学研究院 | 用于检测核酸空间信息的阵列及检测方法 |
CN111440846B (zh) * | 2020-04-09 | 2020-12-18 | 江苏先声医学诊断有限公司 | 一种用于纳米孔测序建库的位置锚定条码系统 |
CN113684247B (zh) * | 2021-08-20 | 2024-05-07 | 深圳市龙华区中心医院 | 一种环状小非编码rna的测序文库构建方法和应用 |
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Effective date of registration: 20180110 Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Applicant after: MGI TECH Co.,Ltd. Address before: Beishan Industrial Zone Building in Yantian District of Shenzhen city of Guangdong Province in 518083 Applicant before: BGI SHENZHEN |
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Address after: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee after: Shenzhen Huada Zhizao Technology Co.,Ltd. Address before: 518083 comprehensive building of Beishan industrial zone and 11 Building 2, Yantian District, Guangdong, Shenzhen Patentee before: MGI TECH Co.,Ltd. |
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