CN105973886B - A kind of method and device determining concentration value based on visible light - Google Patents
A kind of method and device determining concentration value based on visible light Download PDFInfo
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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Abstract
The present invention provides a kind of method and device that concentration value is determined based on visible light, is related to chemical analysis technology field.It is complicated to solve existing assay, the slow problem of acquisition time.Include: that chromogenic reaction is carried out to substance to be determined and standard solution, is chosen from standard solution image and solution image to be determined and determine region, and count the OD value in determining region；It by three primary colours chromatography value is inverted in the OD value of standard solution and is added, and stores into the second document of standard solution, regression fit is carried out to the numerical value in the second document of standard solution, determines the coefficient of regression fit equation；It is chosen from solution image to be determined and determines region, count the OD value determined in region；It by three primary colours chromatography value is inverted in determining OD value and is added, and stores into the second document of solution to be determined, substitutes into the regression fit equation, determine the concentration value of solution to be determined.
Description
Technical field
The present invention relates to the chemical analysis technology fields for passing through spectroscopic methodology detection content, in particular to a kind of to be based on visible light
Spectrum determines the method and device of concentration value.
Background technique
In the measurement analytic process of content of material, the measurement of most of content of material is using spectrophotometry (including enzyme mark
Instrument), spectrophotometry be by measurement measured matter in certain wave strong point or a wavelength range absorbance of light or shine
Intensity, the method that qualitative and quantitative analysis is carried out to the substance.
In spectrophotometer, when the light of different wave length is continuously irradiated to certain density sample solution, it can obtain
To absorption intensity corresponding with different wave length.Such as with wavelength (λ) for abscissa, absorption intensity (A) is ordinate, so that it may be drawn
The absorption spectrum curve of the substance.Qualitative, the quantitative analysis method of substance, referred to as spectrophotometry are carried out using the curve,
Referred to as absorption spectrometry.With the method for ultraviolet source measurement colourless substance, referred to as ultraviolet spectrophotometry；It is surveyed with visible light source
Determine the method for coloring matter, referred to as visible light photometry.It is known as ir spectrophotometry with the content of infrared light supply measurement substance
Method, they are all based on Beer-Lambert law.It is complementary relationship between the light color of color and absorption that substance is presented.
Fig. 1 illustrates the relational graph between solution colour and complementary light, and table 1 illustrates the colour developing of different wave length, is furnished with
Different rgb values.
Table 1, different wave length color correspond to different rgb values
It is mainly measurement with spectrophotometer or microplate reader in the existing method for analyzing content by visible light
Analysis instrument, and in practical applications, since the volume of analysis instrument is bigger, and use condition is more stringent, to lead
It causes assay complicated, is limited by analysis condition, the slow problem of acquisition time.
Summary of the invention
The embodiment of the present invention provides a kind of method and device that concentration value is determined based on visible light, existing for solving
Volume in technology due to analysis instrument is bigger, and use condition is more stringent, so as to cause assay complexity, by point
The limitation of analysis condition, the slow problem of acquisition time.
The embodiment of the present invention provides a kind of method for determining concentration value based on visible light, comprising:
Substance and standard solution to analysis to be determined carry out chromogenic reaction, the solution figure to be determined after obtaining chromogenic reaction
Picture and standard solution image；
First is chosen from the standard solution image got and determines region, and is counted described first and determined in region
OD value；
Described first is determined the red-green-blue chromatography value storage in the OD value in region is molten to the standard
In the first document of liquid；
Complementarity principle is absorbed according to optical wavelength, it is inverted to the data in first document of standard solution and be added, it will
Data that are inverted and being added are stored into second document of standard solution, to the numerical value in second document of standard solution
It carries out regression fit and the coefficient of the regression fit equation is determined according to determining regression fit equation；
Second is chosen from the solution image to be determined and determines region, and is counted described second and determined that the light in region is close
Angle value；Described second is determined the red greenish-yellow three primary colours chromatography value in the OD value in region is stored to solution first to be determined
In document；
Complementarity principle is absorbed according to optical wavelength, it is inverted to the data in first document of solution to be determined and be added,
Data that are inverted and being added are stored into second document of solution to be determined, it will be in second document of solution to be determined
Numerical value substitutes into the regression fit equation, and according to the regression fit equation, determines the concentration value of the solution to be determined.
Preferably, solution and the standard solution image to be determined obtained after chromogenic reaction, comprising:
According to set distance keep the distance between capture apparatus and the solution to be determined or the standard solution, every group
The quantity including the solution to be determined or the standard solution in photo is shot between 5~10.
Preferably, it is described first determine region correspond to multiple reaction tubes same position or multiple volumetric flasks it is same
Position；And/or
Described second determines that region corresponds to the same position at the same position or multiple volumetric flasks of multiple reaction tubes.
Preferably, the numerical value in second document of standard solution carries out regression fit, comprising:
Using the concentration value of the standard solution as ordinate, inverted and phase is worth with the three primary colours chromatography of the standard solution
The data added are abscissa, using following formula, carry out regression fit to the numerical value in second document of standard solution:
Y=a*ln (X)+b
Wherein, known concentration when Y is standard solution sample, X are the three primary colours chromatography optical density inverse accumulated value calculated,
A, b is required coefficient in fitting.
The embodiment of the present invention is a kind of device that concentration value is determined based on visible light, comprising:
Acquiring unit, for the substance and standard solution progress chromogenic reaction to analysis to be determined, after obtaining chromogenic reaction
Solution image and standard solution image to be determined；
Statistic unit determines region for choosing first from the standard solution image got, and described in statistics
First determines the OD value in region；
First storage unit, for determining the red-green-blue chromatography value in the OD value in region by described first
It stores in first document of standard solution；
First determination unit, for absorbing complementarity principle according to optical wavelength, to the number in first document of standard solution
According to inverted and be added, data that are inverted and being added are stored into second document of standard solution, it is molten to the standard
Numerical value in the second document of liquid carries out regression fit and determines the regression fit equation according to determining regression fit equation
Coefficient；
Second storage unit determines region for choosing second from the solution image to be determined, and counts described the
Two determine the OD value in region；Described second is determined the red greenish-yellow three primary colours chromatography value in the OD value in region is deposited
It stores up in the first document of solution to be determined；
Second determination unit, for absorbing complementarity principle according to optical wavelength, in first document of solution to be determined
Data are inverted and are added, and data that are inverted and being added are stored into second document of solution to be determined, will it is described to
Determine in the second document of solution that numerical value substitutes into the regression fit equation, and according to the regression fit equation, determine described in
The concentration value of solution to be determined.
Preferably, the acquiring unit is used for: keeping capture apparatus and the solution to be determined or institute according to set distance
State the distance between standard solution, include in every group of shooting photo the solution to be determined or the standard solution quantity between
5~10.
Preferably, it is described first determine region correspond to multiple reaction tubes same position or multiple volumetric flasks it is same
Position；And/or
Described second determines that region corresponds to the same position at the same position or multiple volumetric flasks of multiple reaction tubes.
Preferably, first determination unit is specifically used for:
Using the concentration value of the standard solution as ordinate, inverted and phase is worth with the three primary colours chromatography of the standard solution
The data added are abscissa, using following formula, carry out regression fit to the numerical value in second document of standard solution:
Y=a*ln (X)+b
Wherein, known content when Y is standard solution sample, X are the three primary colours chromatography optical density inverse accumulated value calculated,
A, b is required coefficient in fitting.
A kind of method and device determining concentration value based on visible light provided in an embodiment of the present invention, including treat really
The substance and standard solution of setting analysis carry out chromogenic reaction, the solution image and standard solution figure to be determined after obtaining chromogenic reaction
Picture；First is chosen from the standard solution image got and determines region, and counts the light in the described first determining region
Density value；Described first is determined the red-green-blue chromatography value in the OD value in region is stored to the standard solution
In first document；Complementarity principle is absorbed according to optical wavelength, it is inverted to the data in first document of standard solution and be added,
Data that are inverted and being added are stored into second document of standard solution, to the number in second document of standard solution
Value carries out regression fit and determines the coefficient of the regression fit equation according to determining regression fit equation；From described to be determined
Second is chosen in solution image and determines region, and counts the OD value in the described second determining region；Described second is determined
Red greenish-yellow three primary colours chromatography value in OD value in region is stored into the first document of solution to be determined；It is apneusis according to light wave
Complementarity principle is received, it is inverted to the data in first document of solution to be determined and be added, by data that are inverted and being added
It stores in second document of solution to be determined, numerical value in second document of solution to be determined is substituted into the regression fit
In equation, and according to the regression fit equation, the concentration value of the solution to be determined is determined.In the embodiment of the present invention, use
Existing digital photographing apparatus (such as camera or mobile phone) takes a picture to standard solution and solution reaction liquid to be determined, will acquire
Image storage to entering in computer, using existing image processing software to the optical density at position same in image, zonule into
Row calculates its average value, the three primary colours determined according to standard solution is stored into the first document of standard solution, according to optical wavelength
Complementarity principle is absorbed, the three primary colours chromatography value in the first document of standard solution is subjected to serial calculating, and after calculating series
Numerical value carries out regression fit, determines regression fit equation coefficient；Above-mentioned processing method is used to solution to be determined, it will be to be determined molten
Numerical value combination regression fit equation after the calculating of liquid series, calculates the concentration value of solution to be determined.Using the above method, Ke Yitong
It crosses simple image processing and numerical value calculates, determine the concentration value of solution to be determined, avoid using the large-scale instrument such as analysis instrument
Device, and above-mentioned determining method has method simple, and the advantage that acquisition time is very fast.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Relational graph of the Fig. 1 between solution colour and complementary light；
Fig. 2 is a kind of method flow schematic diagram that concentration value is determined based on visible light provided in an embodiment of the present invention；
Fig. 3 is standard solution image schematic diagram provided in an embodiment of the present invention；
Fig. 4, which is that standard solution provided in an embodiment of the present invention is selected, determines area schematic；
Fig. 5 is that calculating provided in an embodiment of the present invention determines OD value schematic diagram in region；
Fig. 6 is that the present invention implements to obtain standard solution three primary colours schematic diagram in the image processing process provided；
The regression fit curve synoptic diagram that Fig. 7 standard solution tri-stimulus value provided in an embodiment of the present invention determines；
Fig. 8 is a kind of apparatus structure schematic diagram that concentration value is determined based on visible light provided in an embodiment of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 2 is a kind of method flow schematic diagram that concentration value is determined based on visible light provided in an embodiment of the present invention,
As shown in Fig. 2, it is provided in an embodiment of the present invention it is a kind of based on visible light determine concentration value method the following steps are included:
Step 101, the substance to analysis to be determined and standard solution carry out chromogenic reaction, after obtaining chromogenic reaction to true
Determine solution image and standard solution image；
Step 102, first is chosen from the standard solution image got and determine region, and count described first really
Determine the OD value in region；
Step 103, described first is determined that the red-green-blue chromatography value in the OD value in region is stored to institute
It states in the first document of standard solution；
Step 104, complementarity principle is absorbed according to optical wavelength, it is inverted simultaneously to the data in first document of standard solution
It is added, data that are inverted and being added is stored into second document of standard solution, to second document of standard solution
Interior numerical value carries out regression fit and determines the coefficient of the regression fit equation according to determining regression fit equation；
Step 105, second is chosen from the solution image to be determined and determine region, and count described second and determine region
Interior OD value；Described second is determined the red greenish-yellow three primary colours chromatography value in the OD value in region is stored to be determined
In the first document of solution；
Step 106, complementarity principle is absorbed according to optical wavelength, it is inverted to the data in first document of solution to be determined
And be added, data that are inverted and being added are stored into second document of solution to be determined, by the solution to be determined the
Numerical value substitutes into the regression fit equation in two documents, and according to the regression fit equation, determines the solution to be determined
Concentration value.
In step 101, substance to analysis to be determined and standard solution carry out chromogenic reaction, after obtaining chromogenic reaction to
Determine solution image and standard solution image.
It should be noted that in embodiments of the present invention, to the chromogenic reaction that solution to be determined and standard solution carry out, with
Conventional chemical reaction handling method is consistent, and in embodiments of the present invention, does not do specific limit to the specific method of display reaction
It is fixed.
In practical applications, solution and the standard solution image to be determined after chromogenic reaction is obtained, is needed using general
Capture apparatus shoots image to solution to be determined and standard solution, the image of shooting is determined as to the solution to be determined got
With standard solution image.
It should be noted that needing to infuse when above-mentioned use capture apparatus is to solution to be determined and standard solution shooting image
It anticipates following item:
1), need to guarantee that light source is constant in standard solution or solution electrophotographic process to be determined using camera；
2) it, needs to guarantee camera and standard solution in standard solution or solution electrophotographic process to be determined using camera
Or the distance between solution to be confirmed is constant；
3) before, analyzing, the concentration (or OD value) of solution to be determined is prejudged, it is ensured that the concentration of solution to be determined
It is worth (or OD value) within the scope of concentration of standard solution value (or OD value).
Wherein, the quantity including solution to be determined or standard solution in every group of shooting photo is molten when holding between 5~10
Liquid be test tube when, the quantity in every group of shooting photo including solution to be determined or standard solution can be with 10, when holding solution
Be volumetric flask when, the quantity in every group of shooting photo including solution to be determined or standard solution can be with 5.
For example, the higher mobile phone of pixel can be used or camera is taken a picture, light source is constant when photograph, camera and standard
The distance between solution or solution to be confirmed are determined as 50cm, and the volumetric flask of 5 50ml is put in photograph each time.
In a step 102, it chooses and determines from the standard solution image and the solution image to be determined got
Region, and count the OD value in the determining region.
In practical applications, the standard solution image and solution image to be determined that can be will acquire are in ImageJ software
It is counted in plugins/analyze/RGB measure toolbar, determines the mean optical density of selection area.
Fig. 3 is standard solution image provided in an embodiment of the present invention, in embodiments of the present invention, molten from the standard got
It is chosen in liquid image and determines region, i.e., chosen from the standard solution image that Fig. 3 is provided and determine region, it should be noted that on
It states determining region and corresponds to the same position of multiple reaction tubes or the same position of multiple volumetric flasks.
When being determined region selection, it should be noted that: in test phase and take pictures the stage, standard solution or to be determined
Solution is generally seated among volumetric flask or test tube, and since volumetric flask or test tube different zones correspond to different thickness, i.e. standard is molten
Then the spectrum readings of different parts is also different in optical density for liquid or solution to be determined.In order to avoid there is above situation, one
As in image processing process, need for same section carry out image procossing.
Fig. 4, which is that standard solution provided in an embodiment of the present invention is selected, determines area schematic.As shown in figure 4, being directed in figure
The selected determination region of each volumetric flask is same area, so as to avoid the spectrum value of different parts is different from asking
Topic.
In step 103, by the red-green-blue chromatography value storage in the OD value of the determination to the standard
In the first document of solution；
Fig. 5 is that calculating provided in an embodiment of the present invention determines OD value schematic diagram in region.As shown in figure 5, in this hair
In bright embodiment, using above-mentioned image processing software, can directly acquire will determine the OD value in region, thus the meter saved
Evaluation time, meanwhile, the accuracy of calculated result can be improved in this method.
In practical applications, the first document of standard solution can be excel table, be also possible to other documents, in this hair
In bright embodiment, without limitation to the concrete form of the first document of standard solution.
At step 104, complementarity principle is absorbed according to optical wavelength, the three primary colours chromatography value of determining standard solution is taken down
It counts and is added, the three primary colours chromatography of standard solution is worth data that are inverted and being added and is stored into the second document of standard solution,
Regression fit is carried out to the numerical value in the second document of standard solution, and determines the coefficient of regression fit equation.
Fig. 6 is that the present invention implements to obtain standard solution three primary colours schematic diagram in the image processing process provided, and table 1 is this hair
The information tables such as the standard solution three primary colours that bright embodiment provides.
2 standard solution three primary colours information table of table
In practical applications, inverted with the three primary colours chromatography value of standard solution using concentration of standard solution value as ordinate
And the data being added are abscissa, using formula (1), carry out regression fit to the numerical value in second document of standard solution:
Y=a*ln (X)+b (1)
It should be noted that statistical software can also be used, and using formula (2) in second document of standard solution
Numerical value carry out regression fit:
Y=a+b*exp (c/X) (2)
Wherein, in above-mentioned formula, Y be standard solution sample when known content, X be calculating three primary colours chromatography optical density
Accumulated value reciprocal, a, b, c are required coefficient in fitting.
Fig. 7 is the regression fit curve synoptic diagram that standard solution tri-stimulus value provided in an embodiment of the present invention determines.
It should be noted that above-mentioned steps 101 are into step 104, wherein in step 101, to standard solution and to be determined
The processing that solution carries out, and after step 102 has then only carried out a series of image procossing to standard solution, at algorithm
Reason, without carrying out image procossing and algorithm process to solution to be determined, in following steps 105 and step 106, to be determined
Solution carried out image procossing and some algorithm processing, wherein to solution to be determined carry out image processing method and to standard
The image processing method that solution carries out is consistent, and details are not described herein.
Further, in embodiments of the present invention, it before the concentration value for determining solution to be determined, needs first to be predicted
Examination, guarantees the reaction coloration of solution to be determined within the OD range of standard solution, so as to avoid solution to be determined
The problem of overrich is excessively light, influences definitive result.
It to the algorithm process that solution to be determined carries out, mainly include that numerical value in the second document of solution to be determined is substituted into institute
It states in regression fit equation, wherein two parameters in regression fit equation are determining regression fit side according to standard solution
It is had determined when journey, that is, here, only formula need to be substituted into using the numerical value in the second document of solution to be determined as the X in formula (1)
(1) in, according to formula (1), Y value can be calculated, that is, the concentration value of solution to be determined has been determined.
Table 3 is solution concentration value to be determined provided in an embodiment of the present invention and spectrometer metric results contrast table.
The solution concentration value to be determined of table 3 and spectrometer metric results contrast table
As shown in table 3, the t that pairing mean is V.23 carried out through SPSS is examined, then F=0.062, p=0.805, difference are not shown
It writes.The error for illustrating that determining visible light concentration method provided in an embodiment of the present invention and spectrometer metering method determine all exists
Within normal experimental error, i.e., the method for determining visible light concentration provided in an embodiment of the present invention solves the prior art
In due to the volume of analysis instrument it is bigger, and use condition is more stringent, deposits so as to cause visible light concentration is obtained
In method complexity, the slow problem of acquisition time.
In the embodiment of the present invention, the method for the visible light opacimeter algorithm measurement unknown material concentration provided is applicable in
In 400nm~760nm it is any using spectrophotometer or microplate reader measurement analysis experiment, for example, soil imitate full phosphorus yield,
The measurement of soil available phosphorus, the anthrone method assay of reduced sugar, lactic acid parazon colorimetric method for determining, p-aminophenyl sulphur
Amide method measures content of nitrite, the content of silicon molybdenum blue method measurement silicate, ninhydrin method measurement protein, congo red method and surveys
Determine (ELISA) microplate reader of beta glucan method, the spectrophotometry assay of flavones and replacement horseradish peroxidase colour developing
Assay analysis etc..Method using visible light density calculating method measurement unknown material concentration is easy, and there is certain profession to know
The operator of knowledge can not limited by laboratory apparatus substantially, can complete content of material using popular response system and computer
Measurement.For test error within allowed band, this method is not only very simple and convenient, but also can quickly detect, instead of utilizing
The method of most of substance of visible spectrophotometry measurement.
Based on the same inventive concept, the embodiment of the invention provides a kind of dresses that concentration value is determined based on visible light
It sets, since the principle that the device solves technical problem determines that the method for concentration value is similar based on visible light to a kind of,
The implementation of the device may refer to the implementation of method, and overlaps will not be repeated.
As shown in figure 8, being a kind of device for determining concentration value based on visible light provided in an embodiment of the present invention, packet
It includes: acquiring unit 201, statistic unit 202, the first storage unit 203, the first determination unit 204, the second storage unit 205, the
Two determination units 206.
Single Yuan 201 is obtained, for the substance and standard solution progress chromogenic reaction to analysis to be determined, obtains chromogenic reaction
Solution image and standard solution image to be determined afterwards；
Statistic unit 202 determines region for choosing first from the standard solution image got, and counts institute
State the OD value in the first determining region；
First storage unit 203, for determining the red-green-blue color in the OD value in region by described first
Spectrum is stored into first document of standard solution；
First determination unit 204, for absorbing complementarity principle according to optical wavelength, in first document of standard solution
Data are inverted and are added, and data that are inverted and being added stored into second document of standard solution, to the standard
Numerical value in the second document of solution carries out regression fit and determines the regression fit equation according to determining regression fit equation
Coefficient；
Second storage unit 205 determines region for choosing second from the solution image to be determined, and described in statistics
Second determines the OD value in region；Described second is determined the red greenish-yellow three primary colours chromatography value in the OD value in region
It stores in the first document of solution to be determined；
Second determination unit 206, for absorbing complementarity principle according to optical wavelength, in first document of solution to be determined
Data it is inverted and be added, data that are inverted and being added are stored into second document of solution to be determined, will be described
Numerical value substitutes into the regression fit equation in the second document of solution to be determined, and according to the regression fit equation, determines institute
State the concentration value of solution to be determined.
Preferably, the acquiring unit 201 is used for: according to set distance keep capture apparatus and the solution to be determined or
The distance between described standard solution includes that the quantity of the solution to be determined or the standard solution is situated between in every group of shooting photo
In 5~10.
Preferably, it is described first determine region correspond to multiple reaction tubes same position or multiple volumetric flasks it is same
Position；And/or
Described second determines that region corresponds to the same position at the same position or multiple volumetric flasks of multiple reaction tubes.
Preferably, first determination unit 204 is specifically used for:
Using the concentration value of the standard solution as ordinate, inverted and phase is worth with the three primary colours chromatography of the standard solution
The data added are abscissa, using following formula, carry out regression fit to the numerical value in second document of standard solution:
Y=a*ln (X)+b
Wherein, known content when Y is standard solution sample, X are the three primary colours chromatography optical density inverse accumulated value calculated,
A, b is required coefficient in fitting.
It should be appreciated that this sets according to one of the above determines unit that the device of concentration value includes only based on visible light
The logical partitioning that the function that standby device is realized carries out in practical application, can carry out the superposition or fractionation of said units.And it should
Embodiment provide it is a kind of based on visible light determine function that the device of concentration value is realized with it is provided by the above embodiment
A kind of method one-to-one correspondence determining concentration value based on visible light, the more detailed processing stream realized for the device
Journey has been described in detail in above method embodiment one, has been not described in detail herein.
It should be understood by those skilled in the art that, the embodiment of the present invention can provide as method, system or computer program
Product.Therefore, complete hardware embodiment, complete software embodiment or reality combining software and hardware aspects can be used in the present invention
Apply the form of example.Moreover, it wherein includes the computer of computer usable program code that the present invention, which can be used in one or more,
The computer program implemented in usable storage medium (including but not limited to magnetic disk storage, CD-ROM, optical memory etc.) produces
The form of product.
The present invention be referring to according to the method for the embodiment of the present invention, the process of equipment (system) and computer program product
Figure and/or block diagram describe.It should be understood that every one stream in flowchart and/or the block diagram can be realized by computer program instructions
The combination of process and/or box in journey and/or box and flowchart and/or the block diagram.It can provide these computer programs
Instruct the processor of general purpose computer, special purpose computer, Embedded Processor or other programmable data processing devices to produce
A raw machine, so that being generated by the instruction that computer or the processor of other programmable data processing devices execute for real
The device for the function of being specified in present one or more flows of the flowchart and/or one or more blocks of the block diagram.
These computer program instructions, which may also be stored in, is able to guide computer or other programmable data processing devices with spy
Determine in the computer-readable memory that mode works, so that it includes referring to that instruction stored in the computer readable memory, which generates,
Enable the manufacture of device, the command device realize in one box of one or more flows of the flowchart and/or block diagram or
The function of being specified in multiple boxes.
These computer program instructions also can be loaded onto a computer or other programmable data processing device, so that counting
Series of operation steps are executed on calculation machine or other programmable devices to generate computer implemented processing, thus in computer or
The instruction executed on other programmable devices is provided for realizing in one or more flows of the flowchart and/or block diagram one
The step of function of being specified in a box or multiple boxes.
Although preferred embodiments of the present invention have been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (6)
1. a method of concentration value is determined based on visible light characterized by comprising
Substance to analysis to be determined and standard solution carry out chromogenic reaction, solution image to be determined after obtaining chromogenic reaction and
Standard solution image；
First is chosen from the standard solution image got and determines region, and counts the light in the described first determining region
Density value；
Described first is determined red-green-blue chromatography value storage in the OD value in region to the standard solution the
In one document；
Complementarity principle is absorbed according to optical wavelength, it is inverted to the data in first document of standard solution and be added, it will take down
It counts and the data being added is stored into second document of standard solution, the numerical value in second document of standard solution is carried out
Regression fit determines the coefficient of the regression fit equation according to determining regression fit equation；
The numerical value in second document of standard solution carries out regression fit, comprising: with the concentration of the standard solution
Value is ordinate, is worth data that are inverted and being added as abscissa, using following public affairs using the three primary colours chromatography of the standard solution
Formula carries out regression fit to the numerical value in second document of standard solution:
Y=a*ln (X)+b
Wherein, known concentration when Y is standard solution sample, X are the three primary colours chromatography optical density inverse accumulated value calculated, a, b
For required coefficient in fitting；
Second is chosen from the solution image to be determined and determines region, and counts the optical density in the described second determining region
Value；Described second is determined the red greenish-yellow three primary colours chromatography value storage in the OD value in region is literary to solution first to be determined
In shelves；
Complementarity principle is absorbed according to optical wavelength, it is inverted to the data in first document of solution to be determined and be added, it will take
Data that are reciprocal and being added are stored into second document of solution to be determined, by numerical value in second document of solution to be determined
It substitutes into the regression fit equation, and according to the regression fit equation, determines the concentration value of the solution to be determined.
2. the method as described in claim 1, which is characterized in that the solution image and mark to be determined obtained after chromogenic reaction
Quasi- solution image, comprising: kept between capture apparatus and the solution to be determined or the standard solution according to set distance
Distance includes the quantity of the solution to be determined or the standard solution in every group of shooting photo between 5-10.
3. the method as described in claim 1, which is characterized in that described first determines that region corresponds to the same of multiple reaction tubes
Position or the same position of multiple volumetric flasks；Described second determines that region corresponds to the same position or more of multiple reaction tubes
The same position of a volumetric flask.
4. a kind of device for determining concentration value based on visible light characterized by comprising
Acquiring unit, for analysis to be determined substance and standard solution carry out chromogenic reaction, obtain chromogenic reaction after to
Determine solution image and standard solution image；
Statistic unit determines region for choosing first from the standard solution image got, and counts described first
Determine the OD value in region；
First storage unit, for determining that the red-green-blue chromatography value in the OD value in region is stored by described first
Into first document of standard solution；
First determination unit takes the data in first document of standard solution for absorbing complementarity principle according to optical wavelength
It is reciprocal to be simultaneously added, data that are inverted and being added are stored into second document of standard solution, to the standard solution the
Numerical value in two documents carries out regression fit and determines the coefficient of the regression fit equation according to determining regression fit equation；
First determination unit is specifically used for:
Using the concentration value of the standard solution as ordinate, it is worth with the three primary colours chromatography of the standard solution inverted and addition
Data are abscissa, using following regression formula, carry out regression fit to the numerical value in second document of standard solution:
Y=a*ln (X)+b
Wherein, known content when Y is standard solution sample, X are the three primary colours chromatography optical density inverse accumulated value calculated, a, b
For required coefficient in fitting；
Second storage unit determines region for choosing second from the solution image to be determined, and counts described second really
Determine the OD value in region；Described second is determined the red greenish-yellow three primary colours chromatography value storage in the OD value in region is arrived
In the first document of solution to be determined；
Second determination unit, for absorbing complementarity principle according to optical wavelength, to the data in first document of solution to be determined
It is inverted and be added, data that are inverted and being added are stored into second document of solution to be determined, it will be described to be determined
Numerical value substitutes into the regression fit equation in the second document of solution, and according to the regression fit equation, determines described to true
Determine the concentration value of solution.
5. device as claimed in claim 4, which is characterized in that the acquiring unit is used for: keeping shooting according to set distance
The distance between equipment and the solution to be determined or the standard solution include the solution to be determined in every group of shooting photo
Or the quantity of the standard solution is between 5-10.
6. device as claimed in claim 4, which is characterized in that described first determines that region corresponds to the same of multiple reaction tubes
Position or the same position of multiple volumetric flasks.
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| CN107227257B (en) * | 2017-06-05 | 2020-07-14 | 承启医学（深圳）科技有限公司 | Microbial optical density detection device and detection method |
| CN108519383B (en) * | 2018-04-08 | 2020-08-14 | 上海理工大学 | A detection method for full-band biological color development experiment |
| CN108776107B (en) * | 2018-07-05 | 2021-04-27 | 侯延辉 | Spectrum analysis method for replacing visible spectrophotometer |
| CN110376188B (en) * | 2019-05-30 | 2022-04-22 | 碧沃丰生物科技(广东)股份有限公司 | Inorganic nitrogen detection method based on image analysis |
| CN112557320B (en) * | 2020-11-26 | 2023-06-13 | 中国科学院苏州生物医学工程技术研究所 | Method, system and storage medium for measuring concentration of image colorimetry based on HSV/HSB |
| CN113008722A (en) * | 2021-04-05 | 2021-06-22 | 中国人民解放军63966部队 | Method for measuring dust content in armored vehicle |
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