CN105961399B - Bactericidal composition and its application - Google Patents
Bactericidal composition and its application Download PDFInfo
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- CN105961399B CN105961399B CN201610416462.0A CN201610416462A CN105961399B CN 105961399 B CN105961399 B CN 105961399B CN 201610416462 A CN201610416462 A CN 201610416462A CN 105961399 B CN105961399 B CN 105961399B
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- bactericidal composition
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- 230000004060 metabolic process Effects 0.000 description 1
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052625 palygorskite Inorganic materials 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940104261 taurate Drugs 0.000 description 1
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 1
- GYICYQJEVCIYJY-UHFFFAOYSA-N thiophen-1-ylidenemethanone Chemical compound O=C=S1C=CC=C1 GYICYQJEVCIYJY-UHFFFAOYSA-N 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protection of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of bactericidal composition and its application, it is related to technical field of pesticide combination.The active ingredient of bactericidal composition of the present invention contains shenqinmycin A and bactericide B, and wherein bactericide B is selected from Bravo, fluazinam, and A is 1 with B weight ratio：100~100：1.Effective constituents A has obvious synergistic effect with B compoundings, can effectively reduce the amount of application of each single dose.To expanding fungicidal spectrum and delaying the plant resistance to the action of a drug to have preferable effect, moreover it is possible to extend the lasting period, there is obvious promotional value.Said composition can add the auxiliary element for allowing to use in other agricultural chemicals and be processed into any formulation.The bactericidal composition can effectively prevent and treat rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, root rot, sclerotinia sclerotiorum, maize head smut, droop, downy mildew, epidemic disease, bacterial angular leaf spot etc..
Description
Technical field
The present invention relates to a kind of bactericidal composition and its application, particularly relates to a kind of active ingredient and contains shenqinmycin and hundred
Bacterium is clear or bactericidal composition and its application of fluazinam, belongs to technical field of pesticide combination.
Background technology
In agricultural production, crops can healthy growth, in addition to being influenceed by worm, crop smothering, the preventing and treating to disease is also very heavy
Will.Reinforcement and sustainable development implementation with environmental protection ideas, efficient, low toxicity, high activity, low-residual have turned into agricultural chemicals and sent out
The inexorable trend of exhibition.However, even efficient germicide, long-term to be used alone, also easily disease is developed immunity to drugs, cause to use
The problems such as dose increases, preventive effect reduces, the lasting period shortens, is unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, drop
The dosage of low chemical pesticide, drug effect is improved, turn into the problem that plant protection art is badly in need of solving.
Shenqinmycin, for its structural formula such as shown in (I), shenqinmycin is to be secreted by pseudomonas fluorescens M18 through biological culture
A kind of antibiotic, while there is wide spectrum to suppress phytopathogen and promote the bactericide of the dual-use function of Effects on Plant Growth,
With wide spectrum, it is efficient the characteristics of.
Bravo (chlorothalonil) also known as beat excellent Ke Nier, big kairine, mulberry watt, gram labor, Da Kening, it is mould must
Clearly, daconil etc..Molecular formula：C8N2Cl4, chemical name：Daconil M (the benzene two of 2,4,5,6 one tetrachloro one 1,3 one
Formonitrile HCN).Bravo is a kind of non-internal-absorting wide-spectrum bactericide, has prevention effect to various crop fungal disease.Energy and fungi
Glyceraldehyde 3-phosphate dehydro-genase in cell is had an effect, and is contained with the protein knot containing cysteine in the enzyme body, destructive enzyme
Vigor, the metabolism of fungal cell is destroyed and loss of life power.The main function of Bravo is to prevent plant by true
The infringement of bacterium.Encroached on by germ in plant, after germ enters in plant, bactericidal action root is small.
Fluazinam (Fluazinam), chemical name：The chloro- N- of 3- (the chloro- 5- trifluoromethyls -2- pyridine radicals of 3-)-α, α, α-three
Fluoro- 2,6- dinitro-para-toluidines, molecular formula：C13H4Cl2F6N4O4, fluazinam belongs to 2,6- dinitroanilines, is new
Type broad spectrum protection bactericide, its effect are better than conventional protecting bactericide.To Alternaria, Botrytis, Phytophthora, single shaft
Mould category, Sclerotinia and Venturia germ are highly effective.But the current production of fluazinam and use cost are higher, and
Long-term be used alone also has very big resistance risk.
Banded sclerotial blight is also known as moire disease, and popular name spends sufficient stalk, rotten pin pest, features spot.It is to be fallen ill by Rhizoctonia solani Kuhn infection, exists more
Occur under high temperature, super-humid conditions.Banded sclerotial blight causes harm seriously in South Rice Region of China, is one of the Major Diseases in current Rice Production.
The disease prevents rice from earing, or the not plump paddy of heading is more, and grain declines again.
Plant downy mildew is flagellum mushroom fungal disease.The melon crops such as cucumber, muskmelon, watermelon, cucurbita pepo；Chinese cabbage,
The Cruciferae such as raphanobrassica, cauliflower；Spinach, lettuce and green onion class；The orchard crops such as grape, lichee have downy mildew.Protect
Occur throughout the year in shield ground plastic greenhouse, sporangium can be propagated by air-flow rainwater.Blade disease all can every year caused by downy mildew
Crop 30%-60% economic loss is directly contributed, is that one of serious disease is done in harm in crops.
Frequent use containing single-activity component bactericide, most germs are caused to generate the resistance to the action of a drug, preventive effect is not
Ideal, cause dosage increase, preventive effect reduce, the lasting period shorten the problem of, be unfavorable for environment sustainable development.Therefore, it is badly in need of
A kind of novel product substitutes the sterilised products of single-activity component, and increase drug effect delays the resistance risk of disease, reduces economical
Loss.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of component is reasonable, synergistic effect is notable, and bactericidal effect is good, medication
Cost is low, is not likely to produce the resistance to the action of a drug, the bactericidal composition to crop safety and its application.
In order to solve the above technical problems, present invention offer technical scheme is as follows：
The bactericidal composition of the present invention, its active ingredient is shenqinmycin A and bactericide B, the bactericide B can be hundred
Bacterium is clear or fluazinam, and A and B weight ratio are 1:100~100:1, preferably weight ratio is 1：50~50：1, more preferably weight ratio
For 1：25~25：1.
Pesticidal preparations auxiliary element can also be contained in bactericidal composition of the present invention, be adapted to agriculturally use to be made
Formulation.The pesticidal preparations auxiliary element is one kind in solvent, emulsifying agent, dispersant, stabilizer, antifreezing agent or wetting agent
It is or several.
The bactericidal composition of the present invention can be prepared into any one formulation of suitable agricultural use with known method, than
Preferable formulation is dry suspending agent (DF), oil-suspending agent, aqueous emulsion, microemulsion, wettable powder (WP), water dispersible granules
(WDG), suspending agent (SC), seed treatment or suspension seed-coating agent etc..
A wettable powders (WP), be can be dispersed in water solid powder preparation.Wettable powder of the present invention except
Into active compound plus such as outside white carbon, precipitated calcium carbonate, clay, diatomite, attapulgite diluent or inert substance, also from
Subtype or nonionic surface active agent (wetting agent, dispersant), wetting agent is for example, alkylbenzenesulfonate (DBS-Na), alkyl
Naphthalene sulfonate (pull open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulfate,
Aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether
(JFC), content 0.1~30%；Dispersant such as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), wood
Quality and its derivative sulfonate (M-9, POLYFON etc.), polyoxyethylene poly-oxygen propylene aether block copolymer, alkyl phenol polyoxy second
Alkene ether formaldehyde condensation products sulfate (SOPA), PVA, CMC content 0.1~30%；Also containing penetrating agent JFC, azone, thiophene ketone,
One or more materials among penetrant t, content 0.3~30%；Stabilizer, foam inhibitor, anticaking agent etc. can also be contained
Other auxiliary agents.
B water dispersible granules (WDG), be can be comparatively fast disintegrated in water, the scattered granule for forming higher suspension dispersion.By
The key element such as the auxiliary agent such as active component, wetting agent, dispersant, interleaving agent, disintegrant, stabilizer, adhesive and carrier forms.It is helped
Agent and carrier, and the compound method of precursor (preformed objects before granulation) are analogous to wettable powder and suspending agent substantially.
C suspending agents (SC), it is the not dispersion of water soluble solid agricultural chemicals or immiscible liquids agricultural chemicals in water or oil.By activity
Composition, auxiliary agent (wetting dispersing agent, thickener, stabilizer, pH adjusting agent, defoamer, antifreezing agent) are made through soybean dietary fiber.
Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and its derivative sulfonate
(M-9, POLYFON etc.), alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, alkyl
Phenol polyethenoxy base phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensation phosphorus
One or more in hydrochlorate etc., content 0.1~12%；Thickener and stabilizer can be xanthan gum (XG), polyvinyl alcohol
(PVA), the one or more in Magnesiumaluminumsilicate etc., content 0.05~0.5%；PH adjusting agent and defoamer optionally can add in right amount
Add.
D dry suspending agents (Dry Flowable, abbreviation DF), it is that can be comparatively fast disintegrated in water, scattered form higher suspension and disperse
The granule of system.Preparation method：The preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and precursor (dehydrate
Preceding preformed objects) compound method it is consistent with suspending agent.
Bactericidal composition of the present invention is applied to prepare the pesticidal preparations of controlling plant diseases.Particularly useful for making
Prevent and treat rice sheath blight disease, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice banded sclerotial blight
Disease, root rot, sclerotinia sclerotiorum, maize head smut, capsicum wilt, cucurbits fusarium wilt, downy mildew, epidemic disease, bacterium
The pesticidal preparations of property angular leaf spot, late blight etc..
Bactericidal composition of the present invention can be provided in the form of finished product preparation, i.e., each material has mixed in bactericidal composition
Close, or the composition of bactericidal composition can also be provided with unit dose form, directly directly be mixed in bucket or tank using preceding, so
Required concentration is diluted to afterwards.
Bactericidal composition of the present invention is applied to the method for controlling disease, and active principle A and component B are with above-mentioned weight
Amount is than (1：100-100：1,1：50-50：1,1：25-25：1) apply, it applies after can mixing according to the above ratio, in proportion simultaneously
Using or apply successively in proportion.Specific method of application can be powder injection process, spray-on process, smog method, fumigating system and seed treatment
And soil treatment, ultra-low volume spray, mix fertilizer, spread fertilizer over the fields, pouring root.
Compared with prior art, the beneficial effects of the present invention are：
1st, compared with single dose, compounding has obvious synergistic function, improves prevention effect；
2nd, composition is made up of two kinds of different active ingredients of mechanism of action, and bactericidal effect is good, is advantageous to overcome and delays
The drug-fast generation of germ；
3rd, medicament mixture reduces dosage, so as to reduce cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment plus protective effect, drug cost is low, and its activity and bactericidal effect are not each components
The simple superposition of activity, but have significant synergistic effect, it is good to crop safety, meet the security requirement of pesticidal preparations.
Embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, specific embodiment will be used below
It is described in detail, but the present invention is limited to absolutely not these examples.As described below is only the preferable embodiment of the present invention, only to
The present invention is explained, therefore can not be interpreted as the limitation to the scope of the claims of the present invention.It is it should be pointed out that all the present invention's
All any modification, equivalent and improvement done within spirit and principle etc., should be included in the scope of the protection.
Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Agricultural chemicals is made in the combination of active principles of different agricultural chemicals, is current exploitation and develops novel pesticide and preventing and treating agriculturally
A kind of effective and quick way of resistance germ.After the pestsides synthesis of different cultivars, a large amount of three kinds of effects classes are typically exhibited
Type：Summation action, synergistic effect and antagonism.Compound synergic is formulated well, can significantly improve actual prevention effect, drop
The usage amount of low agricultural chemicals, it is the important means of integrated control disease so as to help to delay the drug-fast generation speed of germ.
For the present composition with shenqinmycin (A) for a kind of active ingredient, bactericide (B) is another active ingredient, they
Between combination there is obvious synergistic function to plant disease, rather than just the simple superposition of two kinds of pharmacy effects, this
It can be clearly understood that from indoor virulence test result.
In order to prevent and treat the rice sheath blight disease in agricultural production, the synergy that inventor is compounded with shenqinmycin and Bravo
Research, specific method are：
Experiment uses rice sheath blight disease as test object.
The test medicine that active compound is configured to need, test method：Fungicide bioassay pattern -- Broad Bean Leaves method,
With reference to《People's Republic of China's agricultural industry criteria》.Selection broad bean susceptible variety is potted plant, and clip same section, growing way is consistent,
With petiolate blade, put in culture dish, moisturizing is standby, and experimental pharmacy is used into active compound water dissolved dilution, sets 5 quality
Concentration, by blade in pre-configured good decoction fully infiltration 5S, unnecessary decoction is drained, after natural air drying, after being marked by processing
Moisturizing culture, experiment set the processing without medicament as blank control.The one side that a diameter of 5mm bacteria cakes have mycelia is connect with inoculator
Kind is in processing central vane, 30 leaves of each processing inoculation.Protectiveness experiment 24h after chemicals treatment is inoculated with, curative tests
24h is inoculated with before chemicals treatment.Growth cabinet or constant temperature are placed in after inoculation to be had in illumination humidistat in fact, Celsius in temperature 26
Cultivated under conditions of -28 degrees Celsius of degree, relative humidity 80%-90%.According to blank control incidence, recorded with kind of calliper
Each vaccination scab length and width, represent that lesion diameter records lesion diameter (unit mm) according to tune with length and width average value
Data are looked into, by preventing and treating result publicity：Preventive effect=(blank diameter-processing diameter)/blank diameter * 100, calculate each processing and suppress disease
The percentage of spot growth, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain each
The EC50 values of medicament, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient meter, reagent agent is evaluated to germ with this
Activity.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect；CTC≤80 show as antagonism；80 ﹤
CTC ﹤ 120 show as summation action.
The Toxicity Determination of the shenqinmycin of table 1 and chlorothalonil compound to rice sheath blight disease
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shenqinmycin (A) | —— | Y=3.4519+0.9042X | 51.5288 | —— |
| Bravo (B1) | —— | Y=3.3471+0.9543X | 53.9566 | —— |
| A：B1 | 1:100 | Y=3.4016+1.0274X | 35.9538 | 150.0 |
| A：B1 | 1:50 | Y=3.7420+0.8475X | 30.5876 | 176.7 |
| A：B1 | 1:25 | Y=3.8288+0.8024X | 28.8175 | 186.9 |
| A：B1 | 1:1 | Y=3.7444+0.8842X | 26.3071 | 200.4 |
| A：B1 | 25:1 | Y=3.8406+0.7886X | 29.5195 | 176.9 |
| A：B1 | 50:1 | Y=3.7073+0.8649X | 31.2408 | 165.1 |
| A：B1 | 100:1 | Y=3.3746+1.0454X | 35.8809 | 143.7 |
Toxicity Determination result table 1 is shown, in weight than 1:100-100:1, particularly 1:50-50:In the range of 1,
Especially in weight than 1：25-25：Shenqinmycin with chlorothalonil compound there is obvious synergy to make to plant disease in the range of 1
With prevention effect is excellent and is better than single dose kind.
In order to prevent and treat the rice blast in agricultural production, inventor is ground with shenqinmycin and the synergy that fluazinam is compounded
Study carefully, specific method is：
Experiment uses rice blast as test object.
Active compound is directly configured to the test medicine of needs with water dissolved dilution, it is standby.Test method (spore germination method).
Cultured disease fungus spore deionized water is eluted from culture medium or diseased tissues, filtered, 5 points is centrifuged, removes supernatant
Liquid, deionized water is added, then centrifuged.Finally spore is resuspended floating to every milliliter of 1*10 with deionized water5-1*107Individual spore, and
Add 0.5% glucose solution.With pipette from low concentration to high concentration, decoction 0.5ml is drawn successively, is separately added into small test tube
In, the spore suspension 0.5ml prepared is then drawn, makes decoction and spore suspension mixed in equal amounts uniform.Use micro sample-adding
Device is drawn above-mentioned mixed liquor and dripped on concave slide, and then frame is put in the culture dish with shallow seated groundwater, and capping moisturizing culture is with fitting
In the incubator of suitable temperature.Each processing is no less than 3 repetitions, and sets the processing without medicament as blank control.
When blank control spore germination rate reaches more than 90%, each processing spore germination situation is checked.Each processing with
Machine observes more than 3 visuals field, and investigation spore sum is no less than 200, records sprouting number and spore sum respectively.Spore germ tube is grown
Degree is considered as sprouting more than the short radius of spore.According to survey data, the spore germination relative inhibition of each processing is calculated.Pass through suppression
Linear regression analysis between the probit value of rate processed and the logarithm value of series concentration, the EC50 values of each medicament are obtained, be with common poison
Number meters calculate the co-toxicity coefficient (CTC) of mixture, and activity of the reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect；CTC≤80 show as antagonism；80 ﹤
CTC ﹤ 120 show as summation action.
The shenqinmycin of table 2 and fluazinam compound the Toxicity Determination to rice blast
Toxicity Determination result table 2 is shown, in weight than 1:100-100:1, particularly 1:50-50:In the range of 1,
Especially in weight than 1：25-25：Shenqinmycin with fluazinam compounding there is obvious synergy to make to plant disease in the range of 1
With prevention effect is excellent and is better than single dose kind.
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, in weight than 1:100~100:
In the range of 1, structure shenqinmycin with Bravo or the embodiment of fluazinam compounding, is described in detail, but the present invention is absolutely respectively
It is not limited to these examples.
In addition, inventor is also found through experiments that, the shenqinmycin compounding effect with Bravo or fluazinam respectively, it is better than
The compounding effect of other antibioticses bactericide and Bravo or fluazinam.In order to clearly demonstrate this problem, in weight ratio
1:100~100:In the range of 1, the comparative example that other antibioticses bactericide compound with Bravo or fluazinam is built, is carried out detailed
Thin description.But because length is limited, representative several comparative examples can only be enumerated.It should be pointed out that shenqinmycin point
Not with the compounding effect of Bravo or fluazinam, imitated better than the compounding of other antibioticses bactericide and Bravo or fluazinam
Fruit, it is not limited in limited comparative example.
The bactericidal composition of the present invention can be prepared into any one formulation of suitable agricultural use with known method, than
Preferable formulation is wettable powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules, microemulsion, aqueous emulsion, kind
Subprocessing agent, suspension seed-coating agent etc..Percentage is weight percentage in all formulas.The various formulations of the present composition add
Work technique is prior art, can be varied from according to different situations.
Present invention is described further with reference to embodiment, comparative example.
One：Shenqinmycin and Bravo
Embodiment 1：20.2% shenqinmycin 〃 Bravos suspending agent (1:100)
Component：Shenqinmycin 0.2%, Bravo 20%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, polycarboxylic acids sodium
3%, 1,2-PD 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 20.2% shenqinmycin Bravo suspending agent is made.
Comparative example 1:20.2% polyoxin 〃 Bravos suspending agent (1:100)
Component：Polyoxin 0.2%, Bravo 20%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, polycarboxylic acids sodium
3%, 1,2-PD 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Ibid.
Embodiment 2:16.8% shenqinmycin 〃 chlorothalonil water dispersible granules (1:55)
Component：Shenqinmycin 0.3%, Bravo 16.5%, sodium lignin sulfonate 5%, polycarboxylic acids sodium 3%, anhydrous slufuric acid
Sodium 8%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 16.8% shenqinmycin chlorothalonil water dispersible granule is made.
Embodiment 3:25.5% shenqinmycin 〃 Bravos wettable powder (1:50)
Component：Shenqinmycin 0.5%, Bravo 25%, dodecyl sodium sulfate 5%, lauryl sodium sulfate 5%, in vain
Carbon black 6%, anhydrous sodium sulfate complements to 100%.
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
25.5% shenqinmycin Bravo wettable powder can be made.
Comparative example 2：25.5% polyoxin 〃 Bravos wettable powder (1:50)
Component：Polyoxin 0.5%, Bravo 25%, dodecyl sodium sulfate 5%, lauryl sodium sulfate 5%, in vain
Carbon black 6%, anhydrous sodium sulfate complements to 100%.
Preparation method：Ibid.
Embodiment 4：27.9% shenqinmycin 〃 Bravos wettable powder (1：45.5)
Component:Shenqinmycin 0.6%, Bravo 27.3%, lauryl sodium sulfate 4%, sodium lignin sulfonate 6%, in vain
Carbon black 8%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
27.9% shenqinmycin 〃 Bravo wettable powders can be made.
Embodiment 5:35% shenqinmycin 〃 chlorothalonil dry suspending agents (1:34)
Component：Shenqinmycin 1%, Bravo 34%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 6%, white carbon
8%, sodium benzoate 0.2%, kaolin supplies 100%.Deionized water 150-200% is additionally added during preparation.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal
It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate, you can it is mould that 35% Shen piperazine is made
Plain chlorothalonil dry suspending agent.
Embodiment 6:26% shenqinmycin 〃 chlorothalonil water dispersible granules (1:25)
Component：Shenqinmycin 1%, Bravo 25%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 5%, anhydrous calcium chloride
3%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 26% shenqinmycin chlorothalonil water dispersible granule is made.
Comparative example 3:26% polyoxin 〃 chlorothalonil water dispersible granules (1:25)
Component：Polyoxin 1%, Bravo 25%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 5%, anhydrous calcium chloride
3%, talcum powder complements to 100%.
Preparation method：Ibid.
Embodiment 7:16% shenqinmycin 〃 Bravos suspending agent (1:15)
Component：Shenqinmycin 1%, Bravo 15%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy second
Alkene ether sulfonic acid sodium 3%, ethylene glycol 5%, XG 0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to
100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 16% shenqinmycin Bravo suspending agent is made.
Embodiment 8：20% shenqinmycin 〃 Bravos wettable powder (1:1)
Component：Shenqinmycin 10%, Bravo 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, hard charcoal
Black 10%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
20% shenqinmycin Bravo wettable powder can be made.
Comparative example 4：20% polyoxin 〃 Bravos wettable powder (1:1)
Component：Polyoxin 10%, Bravo 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, hard charcoal
Black 10%, precipitated calcium carbonate complements to 100%.
Preparation method：Ibid.
Embodiment 9:21% shenqinmycin 〃 chlorothalonil water dispersible granules (19:2)
Component：Shenqinmycin 19%, Bravo 2%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sodium sulfate
5%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 21% shenqinmycin chlorothalonil water dispersible granule is made.
Embodiment 10：16% shenqinmycin 〃 chlorothalonil dry suspending agents (15:1)
Component:Shenqinmycin 15%, Bravo 1%, neopelex 3%, sodium lignin sulfonate 10%, hard charcoal
Black 8%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal
It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 16% Shen piperazine mould
Plain chlorothalonil dry suspending agent.
Embodiment 11:26% shenqinmycin 〃 chlorothalonil water dispersible granules (25:1)
Component：Shenqinmycin 25%, Bravo 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, nothing
Aqueous sodium persulfate 6%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 26% shenqinmycin chlorothalonil water dispersible granule is made.
Comparative example 5:26% polyoxin 〃 chlorothalonil water dispersible granules (25:1)
Component：Polyoxin 25%, Bravo 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, nothing
Aqueous sodium persulfate 6%, talcum powder complements to 100%.
Preparation method：Ibid.
Embodiment 12:18.8% shenqinmycin 〃 Bravos suspending agent (46:1)
Component:Shenqinmycin 18.4%, Bravo 0.4%, polycarboxylic acids sodium 2%, OPEO sodium sulfonate
3%, ethylene glycol 6%, XG0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 18.8% shenqinmycin Bravo suspending agent is made.
Embodiment 13:20.4% shenqinmycin 〃 Bravos suspending agent (50:1)
Component:Shenqinmycin 20%, Bravo 0.4%, sodium naphthalene sulfonate 1%, acrylic acid-acrylic acid hydroxypropyl ester copolymer
4%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.3%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 20.4% shenqinmycin Bravo suspending agent is made.
Comparative example 6：20.4% polyoxin 〃 Bravos suspending agent (50:1)
Component:Polyoxin 20%, Bravo 0.4%, sodium naphthalene sulfonate 1%, acrylic acid-acrylic acid hydroxypropyl ester copolymer
4%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.3%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Ibid.
Embodiment 14:24% shenqinmycin 〃 chlorothalonil water dispersible granules (79:1)
Component:Shenqinmycin 23.7%, Bravo 0.3%, sodium lignin sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous slufuric acid
Sodium 6%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 24% shenqinmycin chlorothalonil water dispersible granule is made.
Embodiment 15:20.2% shenqinmycin 〃 Bravos wettable powder (100:1)
Component：Shenqinmycin 20%, Bravo 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%,
White carbon 8%, kaolin complements to 100%.
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
20.2% shenqinmycin Bravo wettable powder can be made.
Comparative example 7：20.2% polyoxin 〃 Bravos wettable powder (100:1)
Component：Polyoxin 20%, Bravo 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%,
White carbon 8%, kaolin complements to 100%.
Preparation method：Ibid.
Two:Shenqinmycin and fluazinam
Embodiment 16：20.2% shenqinmycin 〃 fluazinams suspending agent (1:100)
Component：Shenqinmycin 0.2%, fluazinam 20%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy
Vinethene 3%, ethylene glycol 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 20.2% shenqinmycin fluazinam suspending agent is made.
Comparative example 8:20.2% jinggangmeisu 〃 fluazinams suspending agent (1:100)
Component：Jinggangmeisu 0.2%, fluazinam 20%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy
Vinethene 3%, ethylene glycol 6%, XG 0.3%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Ibid.
Embodiment 17:21.3% shenqinmycin 〃 fluazinams water dispersible granules (1:70)
Component:Shenqinmycin 0.3%, fluazinam 21%, polycarboxylic acids sodium 4%, lignin sodium naphthalene sulfonate 8%, anhydrous slufuric acid
Sodium 6%, kaolin supplies 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 21.3% shenqinmycin 〃 fluazinam water dispersible granules are made.
Embodiment 18:25.5% shenqinmycin 〃 fluazinams wettable powder (1:50)
Component:Shenqinmycin 0.5%, fluazinam 25%, neopelex 3%, lignin sodium naphthalene sulfonate 6%,
White carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
25.5% shenqinmycin 〃 fluazinam wettable powders can be made.
Comparative example 9：25.5% kasugarnycin 〃 fluazinams wettable powder (1:50)
Component:Kasugarnycin 0.5%, fluazinam 25%, neopelex 3%, lignin sodium naphthalene sulfonate 6%,
White carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method：Ibid.
Embodiment 19：22% shenqinmycin 〃 fluazinam dry suspending agents (1：43)
Component：Shenqinmycin 0.5%, fluazinam 21.5%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 6%, in vain
Carbon black 10%, sodium benzoate 0.2%, talcum powder supplies 100%.Deionized water 150-200% is additionally added during preparation.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal
It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate, you can it is mould that 22% Shen piperazine is made
Plain fluazinam dry suspending agent.
Embodiment 20:35% shenqinmycin 〃 fluazinam dry suspending agents (1:34)
Component：Shenqinmycin 1%, fluazinam 34%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 6%, white carbon
15%, sodium benzoate 0.2%, kaolin supplies 100%.Deionized water 150-200% is additionally added during preparation.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal
It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate, you can it is mould that 35% Shen piperazine is made
Plain fluazinam dry suspending agent.
Embodiment 21:26% shenqinmycin 〃 fluazinams water dispersible granules (1:25)
Component：Shenqinmycin 1%, fluazinam 25%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 6%, anhydrous calcium chloride
2%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 26% shenqinmycin fluazinam water dispersible granules are made.
Comparative example 10:26% polyoxin 〃 fluazinams water dispersible granules (1:25)
Component：Polyoxin 1%, fluazinam 25%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 6%, anhydrous calcium chloride
2%, talcum powder complements to 100%.
Preparation method：Ibid.
Embodiment 22:16% shenqinmycin 〃 fluazinams suspending agent (1:15)
Component：Shenqinmycin 1%, fluazinam 15%, acrylic acid-acrylic acid hydroxypropyl ester copolymer 3%, octyl phenol polyoxy second
Alkene ether sulfonic acid sodium 3%, ethylene glycol 5%, XG 0.35%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to
100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 16% shenqinmycin fluazinam suspending agent is made.
Embodiment 23：20% shenqinmycin 〃 fluazinams wettable powder (1:1)
Component：Shenqinmycin 10%, fluazinam 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, hard charcoal
Black 12%, kaolin complements to 100%.
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
20% shenqinmycin fluazinam wettable powder can be made.
Comparative example 11：20% jinggangmeisu 〃 fluazinams wettable powder (1:1)
Component：Jinggangmeisu 10%, fluazinam 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, hard charcoal
Black 12%, kaolin complements to 100%.
Preparation method：Ibid.
Comparative example 12：20% polyoxin 〃 fluazinams wettable powder (1:1)
Component：Polyoxin 10%, fluazinam 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, hard charcoal
Black 12%, kaolin complements to 100%.
Preparation method：Ibid.
Embodiment 24:21% shenqinmycin 〃 fluazinams water dispersible granules (19:2)
Component：Shenqinmycin 19%, fluazinam 2%, polycarboxylic acids sodium 4%, dodecyl sodium sulfate 6%, anhydrous sodium sulfate
8%, talcum powder complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 21% shenqinmycin fluazinam water dispersible granules are made.
Embodiment 25：16% shenqinmycin 〃 fluazinam dry suspending agents (15:1)
Component:Shenqinmycin 15%, fluazinam 1%, neopelex 3%, lignin sodium naphthalene sulfonate 10%, in vain
Carbon black 8%, organic silicon defoamer 0.4%, sodium benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method：The water of 1.5-2.0 times of preparation amount of preparation is added, each component is mixed, is mixed through high speed shear equal
It is even, then 2-3 hours are sanded by sand mill, average grain diameter is reached 1-5 microns, dehydrate that to can be prepared by 16% Shen piperazine mould
Plain fluazinam dry suspending agent.
Embodiment 26:26% shenqinmycin 〃 fluazinams water dispersible granules (25:1)
Component：Shenqinmycin 25%, fluazinam 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, nothing
Aqueous sodium persulfate 6%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 26% shenqinmycin fluazinam water dispersible granules are made.
Comparative example 13:26% jinggangmeisu 〃 fluazinams water dispersible granules (25:1)
Component：Jinggangmeisu 25%, fluazinam 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, nothing
Aqueous sodium persulfate 6%, kaolin complements to 100%.
Preparation method：Ibid.
Embodiment 27:18.8% shenqinmycin 〃 fluazinams suspending agent (46:1)
Component:Shenqinmycin 18.4%, fluazinam 0.4%, polycarboxylic acids sodium 2%, OPEO sodium sulfonate
3%, ethylene glycol 6%, XG0.32%, organic silicon defoamer 0.2%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 18.8% shenqinmycin fluazinam suspending agent is made.
Embodiment 28:20.4% shenqinmycin 〃 fluazinams suspending agent (50:1)
Component:Shenqinmycin 20%, fluazinam 0.4%, polycarboxylic acids sodium 2%, acrylic acid-acrylic acid hydroxypropyl ester copolymer
3%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.3%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Each component is mixed, is well mixed through high speed shear, then 2-3 hours are sanded by sand mill, makes to put down
Equal particle diameter reaches 1-5 microns, you can 20.4% shenqinmycin fluazinam suspending agent is made.
Comparative example 14：20.4% kasugarnycin 〃 fluazinams suspending agent (50:1)
Component:Kasugarnycin 20%, fluazinam 0.4%, polycarboxylic acids sodium 2%, acrylic acid-acrylic acid hydroxypropyl ester copolymer
3%, ethylene glycol 6%, XG0.3%, organic silicon defoamer 0.3%, sodium benzoate 0.4%, soft water complements to 100%.
Preparation method：Ibid.
Embodiment 29:24% shenqinmycin 〃 fluazinams water dispersible granules (79:1)
Component:Shenqinmycin 23.7%, fluazinam 0.3%, sodium lignin sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous slufuric acid
Sodium 6%, kaolin complements to 100%.
Preparation method：Each component is mixed, through mechanical crushing, then through air-flow crushing, is well mixed, then through wet granulation simultaneously
Drying, you can 24% shenqinmycin fluazinam water dispersible granules are made.
Embodiment 30:20.2% shenqinmycin 〃 fluazinams wettable powder (100:1)
Component：Shenqinmycin 20%, fluazinam 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%,
White carbon 8%, kaolin complements to 100%
Preparation method：Each component is well mixed, first passes through mechanical crushing, then after air-flow crushing, is well mixed, i.e.,
20.2% shenqinmycin fluazinam wettable powder can be made.
Comparative example 15：20.2% polyoxin 〃 fluazinams wettable powder (100:1)
Component：Polyoxin 20%, fluazinam 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%,
White carbon 8%, kaolin complements to 100%
Preparation method：Ibid.
Pesticidal preparations preventing and treating sigatoka, cucumber downy mildew experiment made from above-described embodiment, comparative example.
1st, test process：Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination
Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is the commercially available bacterium of agricultural chemicals 40,%00 respectively
Clear suspending agent, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating and preventing and treating before dispenser
Method is：It is classified and is investigated with blank control incidence, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number
With sick series.
Using following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-15% of whole leaf area；
5 grades：Lesion area accounts for the 16%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Result of the test see the table below 3,4：
The shenqinmycin of table 3 (A) and Bravo (B) compound the prevention effect to sigatoka
The field efficacy result of table 3 shows, in weight than 1：100-100：In the range of 1, shenqinmycin increases with chlorothalonil compound
Positive effect is imitated, prevention effect is better than single dose kind, particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1,
There is excellent prevention effect to sigatoka, prevention effect is better than single dose kind.
Cucumber downy mildew field investigation standard uses following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 5%-10% of whole leaf area；
5 grades：Lesion area accounts for the 11%-25% of whole leaf area；
7 grades：Lesion area accounts for the 26%-50% of whole leaf area；
9 grades：Lesion area accounts for more than the 50% of whole leaf area；
Other Statistical experiments are same as above.
The embodiment of table 4 is compared with comparative example is to the prevention effect of cucumber downy mildew
Inventor has found other antibioticses bactericide and chlorothalonil compound, in weight than 1：100-100：In the range of 1
With certain Complex synergism.Because length is limited, do not enumerate, only constructed pair with most representational polyoxin
Ratio and embodiment carry out preventive effect contrast, as the field efficacy result of table 4 shows, in weight than 1：100-100：In the range of 1, Shen piperazine
Mycin and chlorothalonil compound synergistic effect are obvious, and synergistic effect is better than the preventing and treating of other antibioticses bactericide and chlorothalonil compound
Effect.1：50-50：In the range of 1,1：25-25：In the range of 1, shenqinmycin and chlorothalonil compound synergistic effect are obvious, to planting
The prevention effect of thing disease, it is better than the prevention effect of other antibioticses bactericide and chlorothalonil compound.
Pesticidal preparations preventing and treating pepper anthracnose, late blight of potato experiment made from above-described embodiment, comparative example.
1st, test process：Experiment is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This examination
Test dosing and three concentration for the treatment of are set according to the difference of each composition respectively, comparison medicament is the commercially available fluorine pyridine of agricultural chemicals 50% respectively
Amine suspending agent, 1% shenqinmycin SC and blank clean water experiment.
2nd, test method：Each plot area is 50m2, it is repeated 4 times, the inspection drug effect side after investigating and preventing and treating before dispenser
Method is：It is classified and is investigated with blank control incidence, per the sampling of cell diagonal, often clicks 10 plants, record total strain number, diseased plant number
With sick series.
Using following stage division：
0 grade：It is disease-free；
1 grade：Lesion area accounts for less than the 2% of whole leaf area；
3 grades：Lesion area accounts for the 3%-8% of whole leaf area；
5 grades：Lesion area accounts for the 9%-15% of whole leaf area；
7 grades：Lesion area accounts for the 16%-25% of whole leaf area；
9 grades：Lesion area accounts for less than the 25% of whole leaf area；
Result of the test see the table below 5,6：
The shenqinmycin of table 5 (A) and fluazinam (B) compound the prevention effect to pepper anthracnose
The field efficacy result of table 5 shows, in weight than 1：100-100：In the range of 1, shenqinmycin increases with fluazinam compounding
Positive effect is imitated, prevention effect is better than single dose kind, particularly 1：50-50：In the range of 1, especially 1：25-25：In the range of 1,
There is excellent prevention effect to pepper anthracnose, prevention effect is better than single dose kind.
Late blight of potato field investigation standard uses following stage division：
0 grade：It is disease-free；
1 grade：There are indivedual scabs on indivedual blades；
3 grades：1/3 with the ill spot of lower blade；
5 grades：1/3-1/2 blades have scab；
7 grades：Nearly all blade has scab；
9 grades：Whole blades go rotten almost without green portion；
Other statistics, experimental method are same as above.
The embodiment of table 6 is compared with comparative example is to the prevention effect of the late blight of potato
Inventor has found that other antibioticses bactericide compound with fluazinam, in weight than 1：100-100：In the range of 1
With certain Complex synergism.Because length is limited, do not enumerate, it is only mould with most representational jinggangmeisu, spring thunder
Element, polyoxin construct the progress preventive effect contrast of comparative example and embodiment, as the field efficacy result of table 6 shows, in weight than 1：
100-100：In the range of 1, shenqinmycin and fluazinam Complex synergism are obvious, are better than other antibioticses bactericide and fluorine pyridine
The prevention effect of amine compounding.1：50-50：In the range of 1,1：25-25：In the range of 1, the prevention effect synergy to plant disease is bright
It is aobvious, it is better than other antibioticses bactericide and the prevention effect of fluazinam compounding.
Inventor has found that shenqinmycin has obvious increase to plant disease with Bravo, shenqinmycin and fluazinam compounding
Effect acts on, and tests its prevention effect to root rotof flax.In identical proportion 1:100-100：In 1, particularly 1:50-
50:In the range of 1, especially 1：25-25：In the range of 1, it is found that shenqinmycin is answered with Bravo, shenqinmycin and fluazinam
With also having obvious synergistic effect, there is obvious synergistic effect to plant disease.Because length is limited, it is not repeated.
Described above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (9)
1. a kind of bactericidal composition, it is characterised in that containing effective constituents A and B, wherein A is shenqinmycin, and B is fluazinam, institute
The weight ratio for stating A and B is 1：100~100：1.
2. bactericidal composition according to claim 1, it is characterised in that the effective constituents A and B weight ratio are 1：50
~50：1.
3. bactericidal composition according to claim 2, it is characterised in that the effective constituents A and B weight ratio are 1：25
~25：1.
4. according to any described bactericidal composition in claims 1 to 3, it is characterised in that also contain in the bactericidal composition
There is pesticidal preparations auxiliary element, so that the formulation for being adapted to agriculturally use is made.
5. bactericidal composition according to claim 4, it is characterised in that the pesticidal preparations auxiliary element is solvent, breast
One or more in agent, dispersant, stabilizer, antifreezing agent or wetting agent.
6. bactericidal composition according to claim 4, it is characterised in that the formulation of the bactericidal composition be suspending agent,
Oil-suspending agent, water dispersible granules, wettable powder or suspension seed-coating agent.
7. application of any described bactericidal composition of claim 1 to 6 in the pesticidal preparations for preparing controlling plant diseases.
8. the application of bactericidal composition according to claim 7, it is characterised in that preparing preventing and treating rice sheath blight disease, rice
Seasonal febrile diseases, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, root rot, sclerotinia sclerotiorum, corn
Head smut, capsicum wilt, cucurbits fusarium wilt, downy mildew, epidemic disease, bacterial angular leaf spot, late blight pesticidal preparations in
Using.
9. the application process of any described bactericidal composition of claim 1 to 6, it is characterised in that active principle A and more than B
The weight ratio stated is applied, and it is applied after mixing in proportion, is administered simultaneously in proportion or applied successively in proportion.
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| CN201510262430.5A Active CN104839180B (en) | 2015-01-12 | 2015-05-21 | Fungicide composition and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105104385A (en) * | 2015-09-01 | 2015-12-02 | 河北中天邦正生物科技股份公司 | Bactericide composition and application thereof |
| CN105248432A (en) * | 2015-11-16 | 2016-01-20 | 周红梅 | Anti-fungal composition containing phenazine-1-carboxylic acid and dimethomorph |
| CN105580813A (en) * | 2015-12-18 | 2016-05-18 | 广东中迅农科股份有限公司 | Bactericidal composition containing fluazinam and shenqinmycin |
| CN106577680A (en) * | 2016-12-01 | 2017-04-26 | 青岛东生药业有限公司 | Bactericide and application thereof |
| CN106804158A (en) * | 2016-12-09 | 2017-06-09 | 河池市农业科学研究所 | A kind of rice blast prevention and controls |
| CN109287407A (en) * | 2018-09-14 | 2019-02-01 | 山东省农业科学院农业资源与环境研究所 | A kind of ecological control method of winter wheat root rot |
Citations (4)
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| WO2011076726A2 (en) * | 2009-12-23 | 2011-06-30 | Bayer Cropscience Ag | Pesticidal compound mixtures |
| CN102249799A (en) * | 2011-04-27 | 2011-11-23 | 上海农安生物科技发展有限公司 | Method for controlling crop diseases with shenqinmycin and shenqinmycin preparation |
| CN103444721A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation |
| CN104045601A (en) * | 2014-06-23 | 2014-09-17 | 南京工业大学 | Method for synthesizing phenazine-1-carboxylic acid |
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| JP2005289845A (en) * | 2004-03-31 | 2005-10-20 | Nisso Green:Kk | Method for preventing growth of alga on turfgrass |
| MX2013001837A (en) * | 2010-08-26 | 2013-03-07 | Makhteshim Chem Works Ltd | Synergistic fungicidal composition. |
| CN103960244B (en) * | 2011-12-18 | 2015-09-02 | 深圳诺普信农化股份有限公司 | Bactericidal composition containing Shen piperazine mycin |
| CN103155936B (en) * | 2011-12-18 | 2014-08-20 | 深圳诺普信农化股份有限公司 | Plant disease control composition containing phenazino-1-carboxylic acid |
| CN103155926B (en) * | 2011-12-18 | 2014-04-23 | 深圳诺普信农化股份有限公司 | Sterilization composite based on phenazino-1-carboxylicacid |
| MX360281B (en) * | 2012-05-30 | 2018-10-25 | Bayer Cropscience Ag | Compositions comprising a biological control agent and an insecticide. |
| EP2925141A2 (en) * | 2012-12-03 | 2015-10-07 | Bayer CropScience AG | Composition comprising a biological control agent and a fungicide |
| CN104247716B (en) * | 2013-06-29 | 2016-02-24 | 深圳诺普信农化股份有限公司 | A kind of composition containing Silthiopham |
| CN104054727A (en) * | 2014-06-18 | 2014-09-24 | 北京燕化永乐生物科技股份有限公司 | Sterilization composition containing phenazino-1-carboxylic acid |
| CN104186549A (en) * | 2014-08-20 | 2014-12-10 | 青岛农业大学 | Compound composition preparation of fungicide |
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- 2015-05-21 CN CN201510260961.0A patent/CN104886070B/en active Active
- 2015-05-21 CN CN201610416462.0A patent/CN105961399B/en active Active
- 2015-05-21 CN CN201510262430.5A patent/CN104839180B/en active Active
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| WO2011076726A2 (en) * | 2009-12-23 | 2011-06-30 | Bayer Cropscience Ag | Pesticidal compound mixtures |
| CN102249799A (en) * | 2011-04-27 | 2011-11-23 | 上海农安生物科技发展有限公司 | Method for controlling crop diseases with shenqinmycin and shenqinmycin preparation |
| CN103444721A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Sterilization pesticide composition, pesticide preparation and application of pesticide preparation |
| CN104045601A (en) * | 2014-06-23 | 2014-09-17 | 南京工业大学 | Method for synthesizing phenazine-1-carboxylic acid |
Also Published As
| Publication number | Publication date |
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| CN104839168A (en) | 2015-08-19 |
| CN104839180A (en) | 2015-08-19 |
| CN104839168B (en) | 2017-06-23 |
| CN104886070A (en) | 2015-09-09 |
| CN105961399A (en) | 2016-09-28 |
| CN104839180B (en) | 2017-01-18 |
| CN104886070B (en) | 2017-04-26 |
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