CN105961049B - A kind of optomagnetic processing unit of Ultra-weak Bioluminescence research - Google Patents
A kind of optomagnetic processing unit of Ultra-weak Bioluminescence research Download PDFInfo
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- CN105961049B CN105961049B CN201610352781.XA CN201610352781A CN105961049B CN 105961049 B CN105961049 B CN 105961049B CN 201610352781 A CN201610352781 A CN 201610352781A CN 105961049 B CN105961049 B CN 105961049B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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Abstract
本发明公开了一种生物超微弱发光研究的光磁处理装置，其包括有主控机构以及分别与所述主控机构相接的磁处理组件、光处理组件和电源单元，所述磁处理组件用于对生物进行稳恒磁场或交变磁场的处理，并可根据需要改变磁场的强度或频率等条件，所述光处理组件用于对生物进行光照处理，并可根据需要改变光质、光强、闪频等条件。使用本发明进行超微弱发光测量研究时，可按“磁场形式/磁场强度/频率/作用时间”和“光质/光强/作用时间”等多种设计方案处理植物试样，在对植物无损伤下，了解植物系统内部的生化反应状况、探讨“植物的衰老机理”，为农业育种和栽培技术提供依据，因此本发明应用前景广阔。
The invention discloses an opto-magnetic processing device for biological ultra-weak luminescence research. It is used to treat living beings with a steady and constant magnetic field or an alternating magnetic field, and conditions such as the intensity or frequency of the magnetic field can be changed as required. Strong, flicker and other conditions. When using the present invention to conduct ultra-weak luminescence measurement research, plant samples can be processed according to various design schemes such as "magnetic field form/magnetic field intensity/frequency/action time" and "light quality/light intensity/action time". Under the condition of damage, understanding the biochemical reaction status of the plant system and discussing the "senescence mechanism of plants" provides a basis for agricultural breeding and cultivation technology, so the invention has broad application prospects.
Description
Technical field
The present invention relates to optomagnetic processing unit, specifically a kind of optomagnetic processing dress of Ultra-weak Bioluminescence research
It sets.
Background technique
The phenomenon that superweak luminescence is a kind of universal phenomenon that organism has, the superweak luminescence of organism can provide
The important information of a variety of physiological metabolisms etc. in organism, if plant superweak luminescence and its metabolic physiology are closely related, Ke Yiti
For cell division, photosynthesis, growth and development, the order character of creature and plant by important informations such as extraneous effects.Phase
Biochemical analysis is studied, plant superweak luminescence analysis and research have many advantages, such as, can such as not damage and not interfere plant
The situation for understanding the biochemical reaction inside botanical system in the case where object physiological reaction earlier, can be by the beginning of plant growth
Phase detects Ultra-weak Luminescence, comes the situation and trend of evaluation and foreca plant growth；It can judge to plant according to the luminous intensity of plant seedlings
Winter resistance, heat resistance, salt-resistance and corps nutrient development growth situation of object etc., for agricultural breeding and cultivation technique provide according to
According to.
The superweak luminescence of biology is influenced obviously by extraneous factor, temperature, electromagnetic field, salt stress, H2O2, light quality etc. changes
Becoming can make the superweak luminescence of biology change rapidly.At present using light magetic device to blade carry out light processing (light stress) and
Magnetic field processing (magnetic stress) is two commonly used research means, and the ultraweak hair of plant leaf blade is studied in light processing (light stress)
Light, to " light factor " basic demand be it is photochromic may be selected, intensity of illumination, the processing time can set, however currently used for leaves of plants
" the light stress device " of piece superweak luminescence research, the halogen lamp or fluorescent lamp, white light for such as generating white light are through optical filter " optical filtering "
Or monochromator " light splitting " generation " quasi-monochromatic light " etc., have that " band-like wide range ", device be complicated, light quality/light intensity/time can not interact
The defects of being arranged, be inconvenient for use；Magnetic field handles (magnetic stress) and studies plant leaf blade superweak luminescence, to " the magnetic factor " basic demand
It is that steady magnetic field, alternating magnetic field may be selected, magnetic induction intensity, action time are adjustable, however the research of this aspect is less at present,
It is handled only with steady magnetic field, the problem is that stationary magnetic field is generated by permanent magnet, magnetic cytokine regulatory is inconvenient, using not
Specification.Therefore the backwardness of optomagnetic processing unit research limits the progress of plant superweak luminescence research.
Summary of the invention
The object of the present invention is to provide a kind of optomagnetic processing units of Ultra-weak Bioluminescence research, to solve existing light
Processing unit exist the defects of " band-like wide range ", device be complicated, light quality/light intensity/time can not interact setting, inconvenient for use and
Magnetic processing device magnetic cytokine regulatory is inconvenient, using it is lack of standardization the problems such as.
The purpose of the present invention is what is realized by the following technical solution:
A kind of optomagnetic processing unit of Ultra-weak Bioluminescence research, include main control mechanism and respectively with the master control
Magnetic treatment component, light processing component and the power supply unit that mechanism connects:
The magnetic treatment component includes the mobile pole plate and fixed magnetic pole plate of setting symmetrical above and below, and is arranged in institute
Fixed magnetic pole plate top is stated for containing the container I of test material, the rotating mechanism that the drive container I rotates and described for adjusting
The height adjustment mechanism I of mobile pole plate relative altitude；
The mobile pole plate is that several embeded slots in square matrix-shaped arrangement are equipped on one block of non-magnetic plate, each described
It is equipped in embeded slot and chimeric groove shape, permanent magnet A of the same size；The fixed magnetic pole plate is that have to lead in a center
The non-magnetic plate in hole is equipped with several embeded slots in square matrix-shaped arrangement, is equipped in each embeded slot and chimeric flute profile
Shape, permanent magnet B of the same size；The mobile pole plate is identical as chimeric groove shape, the size on the fixed magnetic pole plate；Institute
The pole N for stating permanent magnet A is extremely opposite with the S of the permanent magnet B；
The container I is made of using un-conducted magnetic material；
Non-magnetic axle sleeve and the stepper motor that the rotating mechanism includes stepper motor, is socketed on the step motor shaft
Driver, the upper through-hole that the non-magnetic axle sleeve passes through the fixed magnetic pole plate are fixed on the bottom center of the container I；Institute
The output end for stating stepper motor driver connects with the stepper motor；
The light processing component includes LED driver, LED combination lamps and lanterns, opposite for adjusting the LED combination lamps and lanterns
The height adjustment mechanism II of height and the container II for being used to contain test material below the LED combination lamps and lanterns；
The output end of the LED driver connects with the LED combination lamps and lanterns；
The LED combination lamps and lanterns include 7 LED lamp beads, 7 optically focused for respectively corresponding setting with each LED lamp bead
Lens and an euphotic cover plate；The wavelength of the 7 LED lamp bead be respectively as follows: 640 ~ 660nm far-red light, 620 ~ 630nm feux rouges,
450 ~ 460nm blue light, 520 ~ 530nm green light, 585 ~ 595nm yellow light, 400 ~ 410nm purple light and main peak are 420 ~ 490nm and 510
The white light of ~ 710nm, half-value angle are 60 degree, and the arrangement of the 7 LED lamp bead is rounded, and even circumferential is distributed 6,1, the center of circle；
The power supply unit is connect with the main control mechanism, the magnetic treatment component, the light processing component respectively, is used for
Voltage needed for the main control mechanism, the magnetic treatment component, the light processing component operation are provided.
Main control mechanism of the present invention includes real-time clock, keyboard, display and single-chip microcontroller, and one of the single-chip microcontroller
Output end connects the display, and two input terminals of the single-chip microcontroller are separately connected the real-time clock and the keyboard；Institute
The another two output end for stating single-chip microcontroller is separately connected the input terminal and the light of stepper motor driver in the magnetic treatment component
The input terminal of LED driver in processing component, for being adjusted by the stepper motor driver to the stepper motor
It controls and the LED combination lamps and lanterns is regulated and controled by the LED driver.
Height adjustment mechanism I of the present invention include guide rail, sliding block supporting plate, rack gear, stem gear, band sliding slot scale and
Handwheel；The sliding block supporting plate is set on the left of the guide rail, and the rack gear is set on the right side of the guide rail, the stem gear with
The rack gear is mutually ratcheting and links together with the sliding block supporting plate, and the axis of the stem gear and the band sliding slot scale are socketed
And the handwheel is installed.
Embeded slot of the present invention in square matrix-shaped arrangement is 16 cylindrical grooves of the same size by 4 × 4 arrangements.
The permanent magnet A of the present invention and permanent magnet B is ndfeb magnet.
Mobile pole plate of the present invention is fixed on the sliding block supporting plate by upper holder, is set on fixed frame
There are several through-holes corresponding with the permanent magnet A；The fixed magnetic pole plate has the lower fixed frame of perforation by a center
It is fixed in a fixing seat, the fixing seat is mounted on lower end on the left of the guide rail；The lower fixed frame be equipped with it is several with
The corresponding through-hole of the permanent magnet B, the through-hole on through-hole and the lower fixed frame on fixed frame are slightly less than it
Corresponding permanent magnet.
The present invention is provided with cooling mechanism above the LED lamp bead of the LED combination lamps and lanterns, and the cooling mechanism is by being close to
The aluminum base PCB plate being arranged above the LED lamp bead and the aluminium base side tooth being set in turn in above aluminum base PCB plate heat dissipation cylinder
And aerofoil fan.
Height adjustment mechanism II of the present invention is to adjust the adjusting being mounted on ∏ type bracket by a locking handwheel
The height of bar is equipped with the LED combination lamps and lanterns in the lower end of the adjusting rod.
Non-magnetic plate used in mobile pole plate of the present invention and the fixed magnetic pole plate is using vulcanie plate
It is made, the container I and the container II are using open-top receptacle made of rigid plastics or non-magnetic stainless steel.
LED driver of the present invention is made of I/O interface circuit and multipath LED drive circuit, every LED lamp bead
It is both connected on the output end of the LED drive circuit；The LED drive circuit uses voltage-dropping type inductor current continuous mode,
Driving chip is SN3352, and the single-chip microcontroller issues pwm signal to control driving chip SN3352 work, is realized to the LED
The luminous regulation of LED lamp bead in combination lamp.
Further, the power of the LED lamp bead is 3W.
Further, low temperature high emission infrared coating is coated on the heat dissipation cylinder.
Using the present invention to biological tissue's test material carry out " magnetic treatment " when, can by change two pieces of magnetic pole distances between plates,
Gradient magnetic is formed on vertical direction, also using stepper motor rotating device, with the rotation of certain revolving speed be placed in two pole plates it
Between container, generate alternating magnetic field in the horizontal plane, realize biological tissue's test material steady magnetic field processing and alternating magnetic field at
Reason, and magnetic field size and processing time can set；When carrying out " light processing " to biological tissue's test material using the present invention, using big
Power LED lamp bead devises LED drive circuit using voltage-dropping type inductor current continuous mode, the serial number that single-chip microcontroller is issued
8 channel parallel datas are converted into according to through I/O interface circuit, are respectively applied to the ADJI pin of driving chip SN3352, realize LED group
Combined lamps shine --- and light quality, light intensity, the interactive regulation of photoperiod carry out at light biology to solve in research process
The some problems occurred during reason or magnetic treatment.
The present invention carry out biological tissue's test material, such as plant leaf blade superweak luminescence measuring study when, can be by " magnetic field
A variety of design schemes such as form/magnetic field strength/frequency/action time " and " light quality/light intensity/action time " handle plant leaf blade,
And then probe into Strength Changes, total number of photons, the attenuation change of delayed luminescence, attenuation parameter 1/ of processing rear blade superweak luminescence
P variation understands the biochemical reaction situation inside botanical system, inquires into " Aging Mechanism of plant ", be under not damaged to plant
Agricultural breeding and cultivation technique provide foundation, therefore the present invention has a extensive future.
The present invention is high-incidence using aluminum side tooth heat dissipation cylinder, aluminum base PCB plate, spraying low temperature when designing LED combination lamps and lanterns
The mode penetrating infrared coating and combining with aerofoil fan, solves the heat dissipation problem of great power LED, guarantees the normal of LED chip
Work.
Detailed description of the invention
Fig. 1 is overall structure block diagram of the present invention.
Fig. 2 is overall structure of the present invention.
Fig. 3 is magnetic treatment modular construction exploded view.
Fig. 4 is light processing modular construction exploded view.
Fig. 5 is LED drive circuit figure in light processing component of the present invention.
Fig. 6 is blade light processing program setting flow chart of the present invention.
In figure: 1, main control mechanism, 1-1, single-chip microcontroller, 1-2, real-time clock, 1-3, display, 1-4, keyboard；2, magnetic treatment
Component, 2-1, mobile pole plate, 2-1-1, vulcanie plate A, 2-1-2, ndfeb magnet A, 2-2, upper holder, 2-3, height
Regulating mechanism I, 2-3-1, sliding block supporting plate, 2-3-2, guide rail, 2-3-3, rack gear, 2-3-4, stem gear, 2-3-5, band sliding slot mark
Ruler, 2-3-6, handwheel, 2-4, fixed magnetic pole plate, 2-4-1, vulcanie plate B, 2-4-2, ndfeb magnet B, 2-5, lower fixation
Frame, 2-6, stepper motor rotating device, 2-6-1, stepper motor, 2-6-2, stepping motor bracket, 2-6-3, non-magnetic axle sleeve, 2-
7, biological tissue's test material container I, 2-8, fixing seat, 2-9, the cabinet with back door；3, light processing component, 3-1, LED driver, 3-
2, LED combination lamps and lanterns, 3-2-1, aluminum base PCB plate, 3-2-2, LED lamp bead, 3-2-3, collector lens, 3-2-4, the heat dissipation of aluminium base side tooth
Cylinder, 3-2-5, euphotic cover plate, 3-2-6, aerofoil fan, 3-3, height adjustment mechanism II, 3-3-1, adjusting rod, 3-3-2, ∏ type
Bracket, 3-3-3, locking handwheel, 3-4, biological tissue's test material container II；4, power supply unit, 5, rack, 6, band back door control cabinet.
Specific embodiment
Embodiment 1
As shown in Figure 1, 2, the present invention is the main control mechanism 1, magnetic treatment component 2, light processing component by being arranged on rack 5
3 and power supply unit 4 constitute, wherein magnetic treatment component 1 is arranged in the left side of rack 5, and the setting of light processing component 3 is on 5 right side of rack
Operating platform above, main control mechanism 1 and power supply unit 4 are installed in the band back door below 5 east side operation platform of rack and control
In case 6.
Main control mechanism 1 includes single-chip microcontroller 1-1, real-time clock 1-2, display 1-3 and keyboard 1-4.Real-time clock 1-2 and
The output end of keyboard 1-4 is separately connected the respective input of single-chip microcontroller 1-1, the input terminal connection single-chip microcontroller 1-1's of display 1-3
Output end；Display 1-3 is LCD display or light-emitting diode display.Band back door control cabinet 6 by operation panel, side panel, bottom plate and
Back door composition, interior to be equipped with device mounting board, wherein single-chip microcontroller 1-1 and real-time clock 1-2 are mounted on device mounting board, display
Device 1-3 and keyboard 1-4 are mounted on operation panel.
Main control mechanism 1 is connected with magnetic treatment component 2, light processing component 3 and power supply unit 4 respectively by cable.
As shown in Figure 1, Figure 3, magnetic treatment component 2 is held by fixed magnetic pole plate 2-4, mobile pole plate 2-1, biological tissue's test material
I 2-7 of device, stepper motor rotating device 2-6 and I 2-3 of height adjustment mechanism are constituted, wherein fixed magnetic pole plate 2-4 and stepper motor
Rotating device 2-6 is mounted in the cabinet 2-9 with back door, specific structure are as follows:
Fixed magnetic pole plate 2-4 is symmetrical above and below with mobile pole plate 2-1 and is arranged in parallel: fixed magnetic pole plate 2-4 is at one piece
The upper surface of vulcanie plate B 2-4-1 offers 16 cylindrical grooves in the same size by 4 × 4 arrangements, in each cylindrical groove
Inside respectively embedded with the pole the S court for ndfeb magnet B 2-4-2, ndfeb magnet the B 2-4-2 being adapted with cylindrical groove size
Upper placement；Mobile pole plate 2-1 is to offer in the lower surface of one piece of vulcanie plate A 2-1-1 by the 16 big of 4 × 4 arrangements
Small consistent cylindrical groove, respectively embedded with the ndfeb magnet A 2-1- being adapted with cylindrical groove size in each cylindrical groove
It places downward the pole N of 2, ndfeb magnet A 2-1-2.The above ndfeb magnet B 2-4-2 and ndfeb magnet A 2-1-2 are
Permanent magnet, a diameter of 20mm, with a thickness of 10mm, adjacent two pieces of magnetic blow out centre spacing 30mm, every piece of magnet surface magnetic induction is strong
Degree is greater than 8000GS, and remanent magnetism is greater than 1T.
I 2-3 of height adjustment mechanism is a screw rod regulating mechanism comprising has sliding block supporting plate 2-3-1, guide rail 2-3-2, tooth
2-3-3, stem gear 2-3-4, with sliding slot scale 2-3-5 and handwheel 2-3-6: the top (left side) of guide rail 2-3-2, which is placed, to be slided
Block supporting plate 2-3-1, bottom (right side) center installation rack gear 2-3-3, by stem gear 2-3-4 and rack gear 2-3-3 it is ratcheting and with cunning
Block supporting plate 2-3-1 links together, and then will be set with sliding slot scale 2-3-5 and stem gear 2-3-4, and be fixed on guide rail 2-3-
Handwheel 2-3-6 is finally mounted on the axis of stem gear 2-3-4 and locks by 2 front.
Mobile pole plate 2-1 passes through upper holder 2-2 respectively and is fixed on sliding block supporting plate 2-3-1, and fixed magnetic pole plate 2-4 is logical
Crossing a center, there is the lower fixed frame 2-5 of perforation to be mounted on guide rail 2-3-2 by fixing seat 2-8；On upper holder 2-2
Through-hole corresponding with the ndfeb magnet A2-1-2 on mobile pole plate 2-1 is offered, and the diameter of the through-hole is slightly less than neodymium
The diameter of iron boron magnetic patch A2-1-2 offers and the ndfeb magnet B2-4-2 on fixed magnetic pole plate 2-4 on lower fixed frame 2-5
Corresponding through-hole, and the diameter of the through-hole is slightly less than the diameter of ndfeb magnet B2-4-2.
Stepper motor rotating device 2-6 be by stepper motor driver, stepper motor 2-6-1, non-magnetic axle sleeve 2-6-3 and
Stepping motor bracket 2-6-2 is constituted: the output end of stepper motor driver connects stepper motor 2-6-1, stepper motor 2-6-1 peace
In stepping motor bracket 2-6-2, axis stretches out stepping motor bracket 2-6-2 and connects with one end of non-magnetic axle sleeve 2-6-3
It connects, the other end of non-magnetic axle sleeve 2-6-3 sequentially passes through the perforation on through-hole and lower fixed frame 2-5 on fixed magnetic pole plate 2-4
Afterwards, it is fixed on the bottom center of I 2-7 of biological tissue's test material container.I 2-7 of biological tissue's test material container is using rigid plastics
Manufactured discoid open-top receptacle.
Magnetic treatment component 2 is connected with power supply unit 4 and main control mechanism 1 respectively by cable, and specific structure is: power supply
Unit 4 connects stepper motor 2-6-1, provides operating voltage for stepper motor 2-6-1；The output of the single-chip microcontroller 1-1 of main control mechanism 1
The input terminal of end connection stepper motor driver, to control the revolving speed of stepper motor 2-6-1.
As Figure 1 and Figure 4, light processing component 3 is by LED driver 3-1, LED combination lamps and lanterns 3-2, height adjustment mechanism II
3-3 and II 3-4 of biological tissue's test material container is constituted, and LED drive circuit structure is as shown in Figure 5.
LED combination lamps and lanterns 3-2 by 7 different colours LED lamp bead 3-2-2,7 collector lens 3-2-3, an euphotic cover
Plate 3-2-5 and cooling mechanism composition: at circumferential, (even circumferential is distributed 6, the center of circle 1 to 7 LED lamp bead 3-2-2 random alignments
), the diameter that a collector lens 3-2-3(collector lens 3-2-3 is correspondingly arranged below every LED lamp bead 3-2-2 is
2.2cm, with a thickness of 1.4cm, optically focused angle is 60 degree), euphotic cover plate 3-2-5 is equipped in the lower section of collector lens 3-2-3；?
The top of LED lamp bead 3-2-2 be equipped with cooling mechanism, the cooling mechanism successively by the aluminum base PCB plate 3-2-1(of abutting LED lamp bead its
Round for diameter 70mm, with a thickness of 3mm), the aluminium base side tooth heat dissipation surface cylinder 3-2-4(be coated with low temperature high emission infrared coating) and
Aerofoil fan 3-2-6 is constituted.The power of the above 7 LED lamp bead 3-2-2 is 3W, and the wavelength of each lamp bead is respectively as follows: 640 ~
660nm far-red light, 620 ~ 630nm feux rouges, 450 ~ 460nm blue light, 520 ~ 530nm green light, 585 ~ 595nm yellow light, 400 ~ 410nm
Purple light, main peak are the white light of 420 ~ 490nm and 510 ~ 710nm, and half-value angle is 60 degree.
The output end of LED driver 3-1 connects LED combination lamps and lanterns 3-2, and LED driver 3-1 is by I/O interface circuit and more
Paths of LEDs driving circuit composition, every LED lamp bead 3-2-2 are both connected on the output end of LED drive circuit；LED drive circuit is adopted
With voltage-dropping type inductor current continuous mode, driving chip SN3352.
II 3-3 of height adjustment mechanism is by the ∏ type bracket 3- on the right side of locking handwheel 3-3-3, adjusting rod 3-3-1 and rack 5
3-2 constitute: adjusting rod 3-3-1 is mounted in the mounting hole on ∏ type bracket 3-3-2, by lock handwheel 3-3-3 adjust its
Relative altitude on ∏ type bracket 3-3-2, LED combination lamps and lanterns 3-2 are mounted on the lower end of adjusting rod 3-3-1.Biological tissue's test material
II 3-4 of container is placed on the operating platform immediately below LED combination lamps and lanterns 3-2, and II 3-4 of biological tissue's test material container is to use
Discoid open-top receptacle made of non-magnetic stainless steel.
Light processing component 3 is connected with power supply unit 4 and main control mechanism 1 respectively by cable, and specific structure is: power supply
Unit 4 connects LED combination lamps and lanterns 3-2, provides voltage for its work；The output end of the single-chip microcontroller 1-1 of main control mechanism 1 connects LED
Driver 3-1, single-chip microcontroller 1-1 issue pwm signal to control driving chip SN3352 work, realize in LED combination lamps and lanterns 3-2
The luminous regulation of LED lamp bead 3-2-2.
According to existing conventional computer technology, pass through keyboard 1-4 and display 1-3 and real-time clock 1-2, single-chip microcontroller 1-
The program set in 1 can regulate and control the speed of rotation of I 2-7 of biological tissue's test material container, realize to biological tissue's test material
Carry out the purpose of alternation magnetic treatment；It rotates handwheel 2-3-6 and adjusts mobile the distance between pole plate 2-1 and fixed magnetic pole plate 2-4,
And " distance --- magnetic field strength " is demarcated, demarcate 0.01 ~ 0.5T range, then can be set as needed vertically to
D.C. magnetic field intensity.
According to existing conventional computer technology, pass through keyboard 1-4 and display 1-3 and real-time clock 1-2, single-chip microcontroller 1-
The program set in 1 can shine to LED combination lamps and lanterns 3-2 --- and light quality, light intensity, photoperiod interact formula regulation, real
The purpose of light processing is now carried out to biological tissue's test material.
The operating method of 2 embodiment of the present invention of embodiment, 1 device
1) magnetic treatment:
1-1) steady magnetic field is handled: adjusting mobile the distance between pole plate 2-1 and fixed magnetic pole plate 2-4 as needed.
Setting vertically to D.C. magnetic field intensity；
1-2) alternating magnetic field is handled: being set the distance between mobile pole plate 2-1 and fixed magnetic pole plate 2-4 first, is then led to
Main control mechanism 1 is crossed, under the control of single-chip microcontroller 1-1 program software, realizes the speed of rotation regulation of stepper motor 2-6-1, it can root
According to the alternating magnetic field intensity for needing to set in horizontal plane；
2) adjusting rod 3-3-1 light processing: is adjusted relative to biological tissue's test material container II by locking handwheel 3-3-3 first
The height of 3-4 under the control of single-chip microcontroller 1-1 program software, realizes LED combination lamps and lanterns 3-2's then by main control mechanism 1
Different light qualities, light intensity, irradiation time and irradiation distance can be set as needed in the regulation of photochromic, light intensity, irradiation time.
3 light quality of embodiment influences the ultraweak spontaneous luminescence of mung bean seedlings blade --- blade light processing
Test material: the mung bean seedlings blade cultivated in illumination cultivation room.
It is set according to program circuit shown in Fig. 6, guarantees that the illuminance on plant leaf blade is identical, specifically set process
Are as follows: 1), power on, start main program, regulation and control model is entered after initialization, display 1-3(is LCD display) it shows first
Interface is welcome, subsequently into system operating mode selection interface, the "lower" key selection " light processing " of keyboard 1-4 is pressed, then presses M key
It determines.2), system time is set: by " left side " " right side " key of keyboard 1-4 when, minute selected, existed with "upper" "lower" key
It is numerically adjusted and determines, after the completion, enter next setting menu by Menu key M.3), dish is set into " model selection "
Single: 1. path settings, the setting of 2. chromaticness, the setting of 3. light intensity, 4. interval settings, the setting of 5. stroboscopics, 6. exit setting；Pass through keyboard
" left side " " right side " key of 1-4 selects " channel-chromaticness-light intensity-light application time-stroboscopic ", changes light intensity value size with "upper" "lower" key
(range 0~99), flicker frequency (range 0~50) presses M key into next setting menu after the completion.4) it, exits setting: " protecting
Deposit modification ", it by " left side " " right side " key, is selected between " confirmation ", " cancellation ", then press M key and determine, system enters work shape
State.
Test method:
(1) blade is put into II 3-4 of biological tissue's test material container, then selects light processing, adjust LED combination lamps and lanterns 3-
2 height relative to II 3-4 of biological tissue's test material container, and main control mechanism 1 is combined to adjust light intensity, guarantee the light on plant leaf blade
Illumination is identical；
(2) selecting uses feux rouges, yellow light, blue and green light and 5 kinds of white light photochromic, after being set by table 1, into work
State；
Table 1:
(3) work terminates, and blade is taken out, and routinely operates with BPCL superweak luminescence analyzer and carries out ultraweak hair
The measurement of luminous intensity.
4 magnetic field of embodiment influences the ultraweak spontaneous luminescence of Kalanchoe blossfediana Leaves --- blade magnetic treatment
Test material: Kalanchoe blossfediana Leaves similar in growing way.
Test method:
(1), for one group, to choose 5 groups of blades to raw a pair of of blade, handwheel 2-3-6 is then rotated, magnetic field strength is set
For 400mT；
(2) first group of blade is placed in I 2-7 of biological tissue's test material container simultaneously, according to the magnetic field strength set, into
Row processing wherein a blade will take out after handling 30min and BPCL superweak luminescence analyzer is used to carry out Weak-luminescence intensity
Measurement, after another blade continues with 30min, use BPCL superweak luminescence analyzer carry out Weak-luminescence intensity survey
It is fixed；Test is divided into 5 groups: first groups of blade magnetic fields times for 30min and 60min, and second group is 45min and 75min, third
Group is 75min and 105min, and the 4th group is 90min and 120min, and the 5th group is 105min and 135min, remaining four groups are pressed phase
Same step carries out.
Claims (10)
1. a kind of optomagnetic processing unit of Ultra-weak Bioluminescence research, characterized in that include main control mechanism and respectively with
Magnetic treatment component, light processing component and the power supply unit that the main control mechanism connects:
The magnetic treatment component includes the mobile pole plate and fixed magnetic pole plate of setting symmetrical above and below, and is arranged described solid
Determine above pole plate for containing the container I of test material, driving the rotating mechanism of the rotation of container I and for adjusting the movement
The height adjustment mechanism I of pole plate relative altitude；
The mobile pole plate is that several embeded slots in square matrix-shaped arrangement are equipped on one block of non-magnetic plate, each described chimeric
It is equipped in slot and chimeric groove shape, permanent magnet A of the same size；The fixed magnetic pole plate is that have through-hole in a center
Non-magnetic plate be equipped with several embeded slots in square matrix-shaped arrangement, be equipped in each embeded slot with chimeric groove shape, greatly
Small consistent permanent magnet B；The mobile pole plate is identical as chimeric groove shape, the size on the fixed magnetic pole plate；It is described forever
The pole N of magnet A is extremely opposite with the S of the permanent magnet B；
The container I is made of using un-conducted magnetic material；
The rotating mechanism includes stepper motor, the non-magnetic axle sleeve being socketed on the step motor shaft and stepper motor driving
Device, the upper through-hole that the non-magnetic axle sleeve passes through the fixed magnetic pole plate are fixed on the bottom center of the container I；The step
Output end into motor driver connects with the stepper motor；
The light processing component includes LED driver, LED combination lamps and lanterns, for adjusting the LED combination lamps and lanterns relative altitude
Height adjustment mechanism II and below the LED combination lamps and lanterns for containing the container II of test material；
The output end of the LED driver connects with the LED combination lamps and lanterns；
The LED combination lamps and lanterns include 7 LED lamp beads, 7 collector lenses for respectively corresponding setting with each LED lamp bead
With an euphotic cover plate；The wavelength of the 7 LED lamp bead be respectively as follows: 640 ~ 660nm far-red light, 620 ~ 630nm feux rouges, 450 ~
460nm blue light, 520 ~ 530nm green light, 585 ~ 595nm yellow light, 400 ~ 410nm purple light and main peak be 420 ~ 490nm and 510 ~
The white light of 710nm, half-value angle are 60 degree, and the arrangement of the 7 LED lamp bead is rounded, and even circumferential is distributed 6,1, the center of circle；
The power supply unit is connect with the main control mechanism, the magnetic treatment component, the light processing component respectively, for providing
Voltage needed for the main control mechanism, the magnetic treatment component, the light processing component operation.
2. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 1, characterized in that the main controller
Structure includes real-time clock, keyboard, display and single-chip microcontroller, and an output end of the single-chip microcontroller connects the display, institute
Two input terminals for stating single-chip microcontroller are separately connected the real-time clock and the keyboard；The another two output end of the single-chip microcontroller point
The defeated of the input terminal of stepper motor driver and the LED driver in the light processing component in the magnetic treatment component is not connected
Enter end, for carrying out regulation and by the LED driver to institute to the stepper motor by the stepper motor driver
LED combination lamps and lanterns are stated to be regulated and controled.
3. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 1 or 2, characterized in that the height
Degree regulating mechanism I includes guide rail, sliding block supporting plate, rack gear, stem gear, band sliding slot scale and handwheel；The sliding block supporting plate is set
Be placed on the left of the guide rail, the rack gear is set on the right side of the guide rail, the stem gear it is mutually ratcheting with the rack gear and with
The sliding block supporting plate links together, and the axis of the stem gear and the band sliding slot scale are socketed and are equipped with the handwheel.
4. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 1, characterized in that described is in square matrix
The embeded slot of shape arrangement is 16 cylindrical grooves of the same size by 4 × 4 arrangements.
5. the optomagnetic processing unit of Ultra-weak Bioluminescence according to claim 1 or 4 research, characterized in that it is described forever
Magnet A and the permanent magnet B are ndfeb magnets.
6. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 3, characterized in that the mobile magnetic
Pole plate is fixed on the sliding block supporting plate by upper holder, and fixed frame is equipped with several opposite with the permanent magnet A
The through-hole answered；The fixed magnetic pole plate by a center there is the lower fixed frame of perforation to be fixed in a fixing seat, described
Fixing seat is mounted on lower end on the left of the guide rail；The lower fixed frame is equipped with several through-holes corresponding with the permanent magnet B,
The through-hole on through-hole and the lower fixed frame on fixed frame is slightly less than the permanent magnet corresponding to it.
7. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 1, characterized in that in the LED
Cooling mechanism, the aluminium that the cooling mechanism is arranged by being close to the LED lamp bead top are provided with above the LED lamp bead of combination lamp
Base pcb board and the aluminium base side tooth being set in turn in above aluminum base PCB plate heat dissipation cylinder and aerofoil fan.
8. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 1 or claim 7, characterized in that the height
Degree regulating mechanism II is the height that the adjusting rod being mounted on ∏ type bracket is adjusted by a locking handwheel, in the adjusting rod
Lower end the LED combination lamps and lanterns are installed.
9. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 1, characterized in that the mobile magnetic
Non-magnetic plate used in pole plate and the fixed magnetic pole plate is made of using vulcanie plate, the container I and the container
II is using open-top receptacle made of rigid plastics or non-magnetic stainless steel.
10. the optomagnetic processing unit of Ultra-weak Bioluminescence research according to claim 2, characterized in that the LED drives
Dynamic device is made of I/O interface circuit and multipath LED drive circuit, and every LED lamp bead is both connected to the LED drive circuit
Output end on；The LED drive circuit uses voltage-dropping type inductor current continuous mode, driving chip SN3352, the list
Piece machine issues pwm signal to control driving chip SN3352 work, and realization shines to the LED combination lamp bulb in LED lamp
Regulation.
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