CN105958096A - Improved microalgae biological direct-driven power generation battery - Google Patents
Improved microalgae biological direct-driven power generation battery Download PDFInfo
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- CN105958096A CN105958096A CN201610038376.0A CN201610038376A CN105958096A CN 105958096 A CN105958096 A CN 105958096A CN 201610038376 A CN201610038376 A CN 201610038376A CN 105958096 A CN105958096 A CN 105958096A
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- 238000010248 power generation Methods 0.000 title abstract description 4
- 239000000126 substance Substances 0.000 claims abstract description 17
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 23
- BTJIUGUIPKRLHP-UHFFFAOYSA-N 4-nitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1 BTJIUGUIPKRLHP-UHFFFAOYSA-N 0.000 claims description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 16
- 239000001301 oxygen Substances 0.000 claims description 16
- 229910052760 oxygen Inorganic materials 0.000 claims description 16
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 claims description 15
- QNVSXXGDAPORNA-UHFFFAOYSA-N Resveratrol Natural products OC1=CC=CC(C=CC=2C=C(O)C(O)=CC=2)=C1 QNVSXXGDAPORNA-UHFFFAOYSA-N 0.000 claims description 15
- LUKBXSAWLPMMSZ-OWOJBTEDSA-N Trans-resveratrol Chemical compound C1=CC(O)=CC=C1\C=C\C1=CC(O)=CC(O)=C1 LUKBXSAWLPMMSZ-OWOJBTEDSA-N 0.000 claims description 15
- 229940016667 resveratrol Drugs 0.000 claims description 15
- 235000021283 resveratrol Nutrition 0.000 claims description 15
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 claims description 14
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 9
- 239000011575 calcium Substances 0.000 claims description 9
- 229910052791 calcium Inorganic materials 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 9
- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 claims description 8
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 8
- 240000002900 Arthrospira platensis Species 0.000 claims description 8
- 239000000470 constituent Substances 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 8
- 229940082787 spirulina Drugs 0.000 claims description 8
- 239000006096 absorbing agent Substances 0.000 claims description 7
- 235000010265 sodium sulphite Nutrition 0.000 claims description 7
- -1 potassium ferricyanide Chemical compound 0.000 claims description 5
- 125000001917 2,4-dinitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C(=C1*)[N+]([O-])=O)[N+]([O-])=O 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 241000195633 Dunaliella salina Species 0.000 claims description 3
- 241000195620 Euglena Species 0.000 claims description 3
- 241001300629 Nannochloropsis oceanica Species 0.000 claims description 3
- 235000007122 Scenedesmus obliquus Nutrition 0.000 claims description 3
- 244000249201 Scenedesmus obliquus Species 0.000 claims description 3
- PVPBBTJXIKFICP-UHFFFAOYSA-N (7-aminophenothiazin-3-ylidene)azanium;chloride Chemical compound [Cl-].C1=CC(=[NH2+])C=C2SC3=CC(N)=CC=C3N=C21 PVPBBTJXIKFICP-UHFFFAOYSA-N 0.000 claims description 2
- KVYRCBOUKXJXDK-UHFFFAOYSA-N 3,4-dimethylphenazine-1,2-diamine hydrochloride Chemical compound Cl.C1=CC=CC2=NC3=C(C)C(C)=C(N)C(N)=C3N=C21 KVYRCBOUKXJXDK-UHFFFAOYSA-N 0.000 claims description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 2
- GBAOBIBJACZTNA-UHFFFAOYSA-L calcium sulfite Chemical compound [Ca+2].[O-]S([O-])=O GBAOBIBJACZTNA-UHFFFAOYSA-L 0.000 claims description 2
- 235000010261 calcium sulphite Nutrition 0.000 claims description 2
- MCPLVIGCWWTHFH-UHFFFAOYSA-L methyl blue Chemical compound [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[NH+]C=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S([O-])(=O)=O)=CC=2)C=C1 MCPLVIGCWWTHFH-UHFFFAOYSA-L 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- FIKAKWIAUPDISJ-UHFFFAOYSA-L paraquat dichloride Chemical compound [Cl-].[Cl-].C1=C[N+](C)=CC=C1C1=CC=[N+](C)C=C1 FIKAKWIAUPDISJ-UHFFFAOYSA-L 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims 1
- 241000168517 Haematococcus lacustris Species 0.000 claims 1
- 229910002651 NO3 Inorganic materials 0.000 claims 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 1
- 230000002745 absorbent Effects 0.000 claims 1
- 239000002250 absorbent Substances 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- GJYLKIZKRHDRER-UHFFFAOYSA-N calcium;sulfuric acid Chemical compound [Ca].OS(O)(=O)=O GJYLKIZKRHDRER-UHFFFAOYSA-N 0.000 claims 1
- 229910001882 dioxygen Inorganic materials 0.000 claims 1
- 239000012286 potassium permanganate Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- 239000000758 substrate Substances 0.000 abstract description 5
- 230000029553 photosynthesis Effects 0.000 abstract description 4
- 238000010672 photosynthesis Methods 0.000 abstract description 4
- 239000002551 biofuel Substances 0.000 abstract description 3
- 239000012528 membrane Substances 0.000 abstract 1
- 239000000446 fuel Substances 0.000 description 9
- 238000002047 photoemission electron microscopy Methods 0.000 description 9
- 229920001483 poly(ethyl methacrylate) polymer Polymers 0.000 description 9
- 241000195493 Cryptophyta Species 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 229920000049 Carbon (fiber) Polymers 0.000 description 2
- 239000012075 bio-oil Substances 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 239000004917 carbon fiber Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 241000195634 Dunaliella Species 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000007772 electrode material Substances 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/8647—Inert electrodes with catalytic activity, e.g. for fuel cells consisting of more than one material, e.g. consisting of composites
- H01M4/8652—Inert electrodes with catalytic activity, e.g. for fuel cells consisting of more than one material, e.g. consisting of composites as mixture
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/02—Details
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M2004/8678—Inert electrodes with catalytic activity, e.g. for fuel cells characterised by the polarity
- H01M2004/8694—Bipolar electrodes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacturing & Machinery (AREA)
- Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Sustainable Energy (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Composite Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a microalgae biological direct-driven power generation battery and particularly relates to a bio-fuel battery with microalgae as an anode electron donor. The battery includes an anode cell and a cathode cell, which are separated from each other by means of a proton exchange membrane therebetween. An anode and a cathode are respectively fixed in the anode cell and the cathode cell and are connected to each other through an external resistor. The microalgae serve as an electron donor in the anode while a common cathode chemical substance or microalgae serves as an electron receptor in the cathode. Through microalgae biological direct-driven power generation, light energy is converted into chemical energy which is then converted into electric energy, namely, with living microalgae as the anode, the light energy is converted into the chemical energy through photosynthesis without any substrate substance, during the process, electrons are led to leak to directly generate electric power. The invention provides a novel bio-fuel battery system.
Description
Technical field
The present invention relates to microalgae biology and directly drive generating battery, relate in particular to the biological fuel cell as anode current donor of microalgae.
Background technology
Inadequate resource due to oil, reduce the increase of supply and continuous cost, the replacement technology finding production capacity becomes the significant interest of research circle, explore the replacement technology about production capacity for many years, algae has been attempted for energy alternate source, the most substantial amounts of data all concentrates in exploitation algae bio oil, thus for synthesising biological fuel.Up to the present, the cost of exploitation algae bio oil is the highest, temporarily cannot compete with petroleum fuel.Showing from document, microalgae was once used in microbiological fuel cell, but microalgae is commonly used in the utilizable substrate of microorganism on positive plate, or microalgae was placed on negative electrode as passive electron acceptor.Though microalgae Anodic Type fuel cell has been reported, but electrically or phycomycete works in coordination with product electrically to produce hydrogen product based on microalgae.Patent CN200910220037 discloses a kind of chlorella biological fuel cell producing electricity based on photosynthesis, it is a kind of indirect method chlorella photodissociation Aquatic product hydrogen system, and microalgae produces hydrogen and can be limited by the anti-inhibitory action of hydrogen and the high cost of metal electrode, easily poisoning.At present, fuel cell studies report electrically is directly produced with microalgae the most fewer.
Summary of the invention
The present invention provides a kind of microalgae biology directly to drive generating battery, it is biological as anode current donor using microalgae, converting luminous energy by photosynthesis is that the directed leakage of electronics directly produces electric power during chemical energy, and device technique and the result of this product electricity are not appeared in the newspapers.
For achieving the above object, the present invention is by the following technical solutions:
A kind of microalgae biology that improves directly drives generating battery, including anode pool and cathode pool, separated by PEM between anode pool and cathode pool, anode and negative electrode are separately fixed at inside anode pool, cathode pool, and connected by non-essential resistance, it is characterized in that: the electron donor of anode is that microalgae is biological, the electron acceptor of negative electrode is cathodic chemical product or microalgae biology, the microalgae of anode is biological guides electronics to leak by the environment controlled residing for microalgae biology, produce electronics, the electronics produced is transferred to negative electrode by external circuit, and is combined generation electric power with the electron acceptor of negative electrode.
Described microalgae biology includes any one in chlorella, Nannochloropsis oceanica, scenedesmus obliquus, very thin Euglena, spirulina maxim, Dunaliella salina, Haematocoocus Pluvialls, spirulina plalensis.
The described environment residing for microalgae biology includes intensity of illumination, microalgae biological cell density, the oxygen level in local environment.
Described intensity of illumination is 1000-10000Lux.Described microalgae biological cell density is 1 × 104-4×107。
Microalgae biology directly drives generating battery and by pumping into nitrogen or can also add oxygen absorber and reduce the oxygen level of anode, and wherein oxygen absorber includes sodium sulfite.
Described cathodic chemical product include that the potassium ferricyanide, described microalgae biology can also add calcium constituent in incubation, and increase electric power, and the addition of calcium constituent is the 0.05%-0.25% cultivating the biological culture medium gross mass of microalgae, and incubation time is 36-50h；Described calcium constituent includes calcium sulfate, calcium sulfite, calcium carbonate.
The biological proton leak that can also carry out inductive anode microalgae bioelectronics transfer chain by adding chemical substance of the microalgae of anode, increasing the leakage of electronics, and increase electric power, the proton of generation is transferred to cathode pool by PEM, described chemical substance includes 2,4-DNP, resveratrol；After wherein 2,4-dinitrophenol,DNP, resveratrol add to the 2,4-dinitrophenol,DNP in system, resveratrol concentration be 250-1000ppm.
Described microalgae biology directly drives generating battery and by adding medium instrumentality or high salt culture medium can also be used to reduce device internal resistance, medium Auto-regulator includes 4-nitroaniline, 4-nitrophenol, methyl blue, dimethyl diaminophenazine chloride, thionine, methyl viologen, humic acid, wherein, 4-nitroaniline, 4-nitrophenol add after to 4-nitroaniline, 4-nitro phenol concentration in system be 5-15ppm.
The microalgae biology that the present invention relates to directly drives generating battery, relate in particular to the biological microalgae biology as anode current donor of a kind of microalgae and directly drive generating battery, including anode pool and cathode pool, separated by PEM between anode pool and cathode pool, anode and negative electrode are separately fixed at inside pond, positive and negative pole, and being connected by non-essential resistance, the electron donor of anode is that microalgae is biological, the electron acceptor of negative electrode is conventional cathodic chemical product or microalgae biology.The present invention is by the way of the microalgae biology direct generation of electricity, convert light energy into chemical energy, it is converted into electric energy again by chemical energy, the microalgae that will live is biological as anode, under there is no substrate, during being chemical energy by photosynthesis conversion luminous energy, the directed leakage of electronics directly produces electric power, it is provided that a kind of novel biofuel cell system.
This microalgae biology directly drives generating battery, improves generating capacity by the way of different, including the distance shortened between anode and negative electrode, changes electrode material, improves electrode surface areas, increases medium conductivity, improve contact point.
Accompanying drawing explanation
Fig. 1 is the equipment drawing that microalgae of the present invention biology directly drives generating battery, and Fig. 1 a is under without external substrate as electric power source, uses microalgae biology as the electron donor directly driving generating battery group, wherein, 1. anode chamber, 2. carbon fiber, 3. pad, 4. PEM, 5. cathode chamber；Fig. 1 b is that simple microalgae biology directly drives generating battery equipment drawing, wherein, 1 ' anode chamber, 2 ' cathode chambers, 3 ' PEMs, 4 ' anode reaction passages.
Fig. 2 is the schematic diagram that microalgae of the present invention biology directly drives generating battery, and wherein, Fig. 2 a is model 1, and the electron donor of its Anodic is that microalgae is biological, and the electron acceptor of negative electrode is the potassium ferricyanide；Fig. 2 b is model 2, and the electron donor of its Anodic is that microalgae is biological, and the electron acceptor of negative electrode is also that microalgae is biological.
Fig. 3 be in Fig. 1 a equipment at the electric current of the lower generation that volume is 200mL.
Fig. 4 is the electric current density produced under distinct device in Fig. 1, wherein, ◆ volume is under Fig. 1 a equipment of 150mL, inside sets the intensity of illumination and 1 × 10 of 3500 luxs6The electric current density of cell concentration；Volume is under Fig. 1 b equipment of 5mL, inside sets the intensity of illumination and 1 × 10 of 3500 luxs6The electric current density of cell concentration
Fig. 5 is the map of current that the embodiment of the present invention 1 (under Fig. 1 a equipment) Chlorella pyrenoidesa directly drives that generating battery produces under different controlled conditions, and in Fig. 5 a, cell density is 5.94x 106During cells/ml, the map of current produced under different level conditions, wherein ■ 2500 lux, ▲ 3500 luxs, ◆ 6500 luxs；In Fig. 5 b, illuminance is 3500 luxs, the map of current produced under the conditions of different cell densities, wherein ◆ 3.68x 107, 3.08x 107, △ 1.39x 107, ● 5.94x 106Cells/ml.
Fig. 6 is not for add Na2SO3Under, in anode chamber 5.94 × 106Biological dissolved oxygen (DO) mg/L under different light intensities of the microalgae of cells/ml, wherein ◆ 2500 luxs；■ 3500 lux；▲ 6500 luxs；Along with adding 1g/LNa2SO3Under, in anode chamber 5.94 × 106Biological dissolved oxygen (DO) mg/L under different light intensities of the microalgae of cells/ml, wherein ◇ 2500 lux；3500 lux；△ 6500 lux.
Fig. 7 is that the embodiment of the present invention 2 Chlorella pyrenoidesa directly drives after generating battery adds sodium sulfite in anode pool, and the map of current produced under different controlled conditions, in Fig. 7 a, cell density is 5.94x 106During cells/ml, the map of current produced under different level conditions, wherein ■ 2500 lux, ▲ 3500 luxs, ◆ 6500 luxs；In Fig. 7 b, illuminance is 3500 luxs, the map of current produced under the conditions of different cell densities, wherein ◆ 3.68x 107, 3.08x 107, △ 1.39x 107, ● 5.94x 106Cells/ml.
Fig. 8 is the intensity of illumination of 3500 luxs, does not adds Na2SO3Under, biological dissolved oxygen (DO) mg/L under different cell densities of microalgae, wherein, ◆ 5.95x106、■1.39x107、●3.08x107、▲3.68x107；Along with adding 1g/LNa2SO3Under, biological dissolved oxygen (DO) mg/L, wherein ◇ 5.95x10 under different cell densities of microalgae under the intensity of illumination of 3500 luxs6、□1.39x107、○3.08x107、△3.68x107。
Fig. 9 is the impact that Chlorella pyrenoidesa is directly driven generating battery by the embodiment of the present invention 3 resveratrol (RVT), 2,4-dinitrophenol,DNP (DNP), 4-nitroaniline (4NA), 4-nitrophenol (4NP).Fig. 9 a is to be added without and add the map of current under the conditions of resveratrol (RVT) and 2,4-DNP (DNP), and wherein ■ does not adds RVT and DNP, ◆ add RVT and DNP；Fig. 9 b is the voltage pattern under the conditions of addition 4-nitroaniline (4NA), and wherein ◇ 5ppm 4NA, ■ add 10ppm 4NA, △ 15ppm 4NA.Fig. 9 c is the voltage pattern under the conditions of addition 4-nitrophenol (4NP), and wherein ◇ adds 5ppm 4NP, ■ and adds 10ppm 4NP, △ 15ppm 4NP.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, further illustrate various embodiments of the present invention below with reference to accompanying drawing.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment 1
Fig. 1 is the equipment drawing that microalgae of the present invention biology directly drives generating battery, and Fig. 1 a is under without external substrate as electric power source, uses microalgae biology as the electron donor directly driving generating battery group, wherein, 1. anode chamber, 2. carbon fiber, 3. pad, 4. PEM, 5. cathode chamber；Fig. 1 b be Fig. 1 b be that simple microalgae biology directly drives generating battery equipment drawing, wherein, 1 ' anode chamber, 2 ' cathode chambers, 3 ' PEMs, 4 ' anode reaction passages.
As shown in Figure 2 a, the present invention the 1st microalgae biology described in embodiment directly drives generating battery and includes the internal anode pool 1 biological equipped with microalgae and add the cathode pool 2 of oxidizing agent solution, separated by PEM 3 between two ponds, anode 4 and negative electrode 5 are separately fixed at inside anode pool and cathode pool, and are connected by external circuit 6.The microalgae of anode is biological guides electronics to leak by the environment controlled residing for microalgae biology, produce electronics, the electronics produced is transferred to negative electrode by external circuit, and generation electric power is combined with the electron acceptor of negative electrode, the microalgae biology used in this embodiment is Chlorella pyrenoidesa, its cultural method is for using TAP culture medium culturing, culture bottle covers the filter of 0.22 μm, and cultivate 3 days at the environmental chamber persistently rotated with 120rpm, this environmental chamber keeps 25 DEG C of constant temperature, and uses the circulation light of the 3500 luxs/dark of 16/8h to shine.
Fig. 5 a is when Chlorella pyrenoidesa cell density is 5.94 × 106During cells/ml, respectively in illuminance 2500 lux, 3500 luxs, under the conditions of 6500 luxs, the electric current that Chlorella pyrenoidesa produces, current data average ± standard error represents.Illuminance is when 2500 lux, and the current intensity of generation is maximum, and initial current maximum intensity is 28 μ A, is gradually lowered and is maintained at 5 μ A subsequently.
Fig. 5 b is for when illuminance is 3500 lux, and Chlorella pyrenoidesa density is respectively 3.68 × 107, 3.08 × 107, 1.39 × 107, 5.94 × 106Under the conditions of cells/ml, the electric current that Chlorella pyrenoidesa produces, current data average ± standard error represents.Microalgae cell density is 5.94x 106During cells/ml, the current intensity of generation is maximum, and initial current maximum intensity is 28 μ A, is gradually lowered and is maintained at 5 μ A subsequently.
Embodiment 2
It is same as in Example 1 that described microalgae directly drives generating battery, difference is in anode pool to add oxygen absorber sodium sulfite (1 grams per liter), Fig. 7 a is to add oxygen absorber sodium sulfite (1 grams per liter) in anode pool, and Chlorella pyrenoidesa cell density is 5.94 × 106During cells/ml, respectively in illuminance 2500 lux, 3500 luxs, under the conditions of 6500 luxs, the electric current that Chlorella pyrenoidesa produces, current data average ± standard error represents.Illuminance is when 2500 luxs and 3500 lux, and the initial current intensity produced is relatively big, and accessible maximum is respectively 70 μ A and 78 μ A, and stable at 60 μ A after 100min.
Fig. 7 b is to add oxygen absorber sodium sulfite (1 grams per liter) in anode pool, and when illuminance is 3500 lux, Chlorella pyrenoidesa cell density is respectively 3.68 × 107, 3.08 × 107, 1.39 × 107, 5.94 × 106Under the conditions of cells/ml, the electric current that Chlorella pyrenoidesa produces, current data average ± standard error represents.Microalgae cell density is 5.94 × 106During cells/ml, the initial current intensity produced is maximum, and the maximum reached is 78 μ A and stablizes at 55 μ A at 100min.
Embodiment 3
It is same as in Example 1 that described microalgae directly drives generating battery, and difference is add 250ppmRVT and 250ppmDNP in a fuel cell or add (5-15) ppm4NA, or adds (5-15) ppm 4NP.Fig. 9 a is to add 250ppm RVT and 250ppmDNP and scheme without the current vs under the conditions of RVT and DNP, wherein add the 1000ppmDNP of 1ml at 45min after, electric current is increased to 9 μ A by 3 μ A, there is identical phenomenon in the 1000ppmDNP being separately added into 1ml at two minor tick 30min, after the 30min of third time interval, adding the 1000ppm RVT of 1ml, electric current is increased to 8 μ A by 3 μ A；Fig. 9 b is the voltage-contrast figure under the conditions of adding (5-15) ppm4-nitroaniline or being added without 4NA, and the microalgae battery voltage after 30min wherein adding 4NA starts to increase.Fig. 9 c is the voltage-contrast figure under the conditions of adding (5-15) ppm 4-nitrophenol or being added without 4NP, and the microalgae battery voltage after 30min wherein adding 4NP starts to increase.
Embodiment 4
As shown in Figure 2 b, the present invention the 4th microalgae biology described in embodiment directly drives generating battery and includes the internal anode pool 1 biological equipped with microalgae and the cathode pool 2 of microalgae biology, separated by PEM 3 between two ponds, anode 4 and negative electrode 5 are separately fixed at inside anode pool and cathode pool, and are connected by external circuit 6.The microalgae of anode is biological guides electronics to leak by the environment controlled residing for microalgae biology, produce electronics, the electronics produced is transferred to negative electrode by external circuit, and is combined with the electron acceptor of negative electrode and produces the oxygen combination generation water of release in electric power, and the microalgae organism in cathode pool.
Model 1: the electron donor of anode is Chlorella pyrenoidesa, the electron acceptor of negative electrode is six cyano iron ions；Model 2: the electron donor of anode is Chlorella pyrenoidesa, the electron acceptor of negative electrode is Chlorella pyrenoidesa.Fig. 8 shows to utilize microalgae biology directly to drive generating battery, chemical energy can be converted into electric energy, under certain controlled condition, anode pool i.e. add oxygen absorber sodium sulfite (1 grams per liter), illuminance is 3500 luxs, and Chlorella pyrenoidesa cell density is 5.94 × 106During cells/ml, the output power density of model 1 can reach 30.15mW/m2, 6030mW/m2/L；The output power density of model 2 can reach 2.16mW/m2, 432mW/m2/L。
Following table is the comparing result of the present invention and other microbiological fuel cell generating capacity:
Embodiment 5
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is chlorella, uses TAP culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 6
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is Nannochloropsis oceanica, uses F/2medium culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 7
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is scenedesmus obliquus, uses TAP culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 8
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is spirulina maxim, uses spirulina medium culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 9
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is very thin Euglena, uses HUT medium culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 10
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is Dunaliella salina, uses Dunaliella medium culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 11
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is Haematocoocus Pluvialls, uses BBM culture medium to cultivate, and cultural method is with embodiment 1.
Embodiment 12
In the present embodiment, microalgae directly drives that generating battery is same as in Example 1, and difference is, the microalgae biology used is spirulina plalensis, uses spirulina medium culture medium to cultivate, and cultural method is with embodiment 1.
In above-mentioned middle embodiment 5-12, the electric power result using different algal species to produce is as follows:
Embodiment 10
In the present embodiment, it is same as in Example 1 that microalgae directly drives generating battery, difference is, the microalgae biology used is chlorella, uses TAP culture medium to cultivate, and cultural method is with embodiment 1, TAP culture medium is wherein added calcium constituent again and cultivates 48h, the addition of calcium constituent accounts for the 0.05% of culture medium total amount, 0.25%, and it is 0.05%, 0.1%, 0.2%, 0.25% that the application drafts, and the electric power result of generation is as follows:
As can be seen from the table, add calcium and cultivate 48 hours to algae (chlorella), bring up to 2～3 times in power generation.
Claims (10)
1. microalgae biology directly drives a generating battery, including anode pool and cathode pool, between anode pool and cathode pool
Being separated by PEM, anode and negative electrode are separately fixed at inside anode pool, cathode pool, and by external electrical
Resistance connects, it is characterised in that: the electron donor of anode is that microalgae is biological, and the electron acceptor of negative electrode is cathodic chemical
Product or microalgae are biological, and the microalgae of anode is biological guides electronics to leak by the environment controlled residing for microalgae biology, produces
Raw electronics, the electronics of generation is transferred to negative electrode by external circuit, and is combined generation electric power with the electron acceptor of negative electrode.
2. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that described microalgae is raw
Thing includes chlorella, Nannochloropsis oceanica, scenedesmus obliquus, very thin Euglena, spirulina maxim, Dunaliella salina, rain
Any one in raw Haematococcus Pluvialis, spirulina plalensis.
3. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that described microalgae is raw
Environment residing for thing includes intensity of illumination, microalgae biological cell density, the oxygen level in local environment.
4. microalgae biology as claimed in claim 3 directly drives generating battery, it is characterised in that described illumination is strong
Degree is 1000Lux-10000Lux.
5. microalgae biology as claimed in claim 3 directly drives generating battery, it is characterised in that described microalgae is raw
Thing cell density is 1 × 104-4×107。
6. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that microalgae biology directly drives
Generating battery by pumping into nitrogen or can also add oxygen absorber and reduces the oxygen level of anode, wherein oxygen
Gas absorbent includes sodium sulfite.
7. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that described is cathodization
Product include the potassium ferricyanide, potassium permanganate, nitrate, sulfate, microalgae biology or air.
8. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that described microalgae is raw
Thing can also add calcium constituent in incubation, and increases electric power, and the addition of calcium constituent is raw for cultivating microalgae
The 0.05%-0.25% of the culture medium gross mass of thing, incubation time is 36-50h；Described calcium constituent includes sulphuric acid
Calcium, calcium sulfite, calcium carbonate.
9. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that the microalgae of anode
The biological proton leak that can also induce microalgae bioelectronics transfer chain by adding chemical substance, increases electronics
Leakage, and increase electric power, the proton of generation is transferred to cathode pool, described chemical substance by PEM
Including 2,4-dinitrophenol,DNP, resveratrol；To system after wherein 2,4-dinitrophenol,DNP, resveratrol add
In 2,4-dinitrophenol,DNP, resveratrol concentration be 250-1000ppm.
10. microalgae biology as claimed in claim 1 directly drives generating battery, it is characterised in that microalgae is biological straight
Driving generating battery by adding medium instrumentality or can also use high salt culture medium to reduce device internal resistance, medium
Auto-regulator includes 4-nitroaniline, 4-nitrophenol, methyl blue, dimethyl diaminophenazine chloride, thionine, methyl viologen, corruption
Grow acid, wherein, to 4-nitroaniline, 4-nitrophenol in system after the interpolation of 4-nitroaniline, 4-nitrophenol
Concentration is 5-15ppm.
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