CN105941115A - Rice coleoptiles and content extraction method and application thereof - Google Patents
Rice coleoptiles and content extraction method and application thereof Download PDFInfo
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 114
- 235000009566 rice Nutrition 0.000 title claims abstract description 114
- 238000000605 extraction Methods 0.000 title abstract 3
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 113
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 101710162629 Trypsin inhibitor Proteins 0.000 claims abstract description 14
- 229940122618 Trypsin inhibitor Drugs 0.000 claims abstract description 14
- 239000002753 trypsin inhibitor Substances 0.000 claims abstract description 14
- 238000003306 harvesting Methods 0.000 claims abstract description 10
- 230000010261 cell growth Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract 3
- 239000000284 extract Substances 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 8
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- 206010028980 Neoplasm Diseases 0.000 claims description 5
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- 238000012364 cultivation method Methods 0.000 claims description 5
- 235000013402 health food Nutrition 0.000 claims description 5
- 230000001681 protective effect Effects 0.000 claims description 5
- 150000003254 radicals Chemical class 0.000 claims description 4
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 239000003729 cation exchange resin Substances 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000003814 drug Substances 0.000 claims description 3
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 230000011218 segmentation Effects 0.000 claims description 3
- 238000003809 water extraction Methods 0.000 claims description 3
- 230000003712 anti-aging effect Effects 0.000 claims description 2
- 239000002537 cosmetic Substances 0.000 claims description 2
- 210000001161 mammalian embryo Anatomy 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 17
- 108091003079 Bovine Serum Albumin Proteins 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000012894 fetal calf serum Substances 0.000 abstract 1
- 238000000265 homogenisation Methods 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 230000003068 static effect Effects 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- 230000001629 suppression Effects 0.000 description 12
- 206010002660 Anoxia Diseases 0.000 description 8
- 241000976983 Anoxia Species 0.000 description 8
- 206010021143 Hypoxia Diseases 0.000 description 8
- 230000007953 anoxia Effects 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- 239000003112 inhibitor Substances 0.000 description 7
- 108090000631 Trypsin Proteins 0.000 description 6
- 102000004142 Trypsin Human genes 0.000 description 6
- 230000003078 antioxidant effect Effects 0.000 description 6
- 230000002786 root growth Effects 0.000 description 6
- 239000012588 trypsin Substances 0.000 description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 4
- 239000005030 aluminium foil Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 230000003301 hydrolyzing effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 229930003427 Vitamin E Natural products 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 229960000271 arbutin Drugs 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
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- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 239000011709 vitamin E Substances 0.000 description 2
- 235000019165 vitamin E Nutrition 0.000 description 2
- 230000002087 whitening effect Effects 0.000 description 2
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000002333 glycines Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- TWTIGXOHHSOAJN-UHFFFAOYSA-N n-[3-[4-(3-aminopropylamino)butylamino]propyl]benzamide Chemical compound NCCCNCCCCNCCCNC(=O)C1=CC=CC=C1 TWTIGXOHHSOAJN-UHFFFAOYSA-N 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000003519 ventilatory effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/55—Protease inhibitors
- A61K38/56—Protease inhibitors from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Gastroenterology & Hepatology (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Birds (AREA)
- Pharmacology & Pharmacy (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Environmental Sciences (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a rice coleoptiles and a content extraction method and application thereof, and belongs to the coleoptiles culture and application technical field; the rice coleoptiles is cultured by the following steps: rice disinfection and sterilization; static culturing; exchanging water when rice coleoptiles starts to emerge, contentiously settling without changing water, and isolating air, so the rice coleoptiles can grow in an anoxic environment; harvesting rice coleoptiles. The rice coleoptiles content extraction method comprises the following steps: homogenization, separating macro-molecule from micro-molecule, and separating trypsin inhibitor; the rice coleoptiles content extractant of 454ug/ml is added in a 10% fetal calf serum medium, thus providing 37% substantial inhibition on HK-2cells growth; in addition, the rice coleoptiles content extractant of 909.1ug/ml is added to inhibit 27% of cell growth rate.
Description
Technical field
The present invention relates to bud scale cultivate and applied technical field, particularly to the application in having in preparation in the health food of protective effect on cancer risk and preparing the medicine with cell growth inhibiting rate action of a kind of rice bud scale, the content extracting process of rice bud scale and this rice bud scale.
Background technology
Purification and characteristic by learning rice bud scale trypsin inhibitor in published Master's thesis (paper identification code: etd-0730103-180759): the rice bud scale of growth from immersion conditions environmental, can isolate a kind of molecular weight about at the trypsin inhibitor of 18kDa via the mode of cation exchange column and colloid col-umn chromatography.
This inhibitor is suppressed a behavior not typical competitive type suppression reaction for chymotrypsin protein enzyme hydrolysis by matter N-benzoyl-spermine acid-paranitroanilinum (L-BAPNA)；But its suppression mechanism by matter same for trypsin hydrolyzing is identical with the trypsin inhibitor extracted from Semen sojae atricolor, broadly fall into the suppression reaction of competitive type.
The complex (EI) that the inhibitor obtained from rice bud scale is combined with trypsin, its dissociation constant is 4.0 × 10-7M, and the complex (EI) that the inhibitor extracted from Semen sojae atricolor is combined with same ferment, its dissociation constant is 7.4X 10-7M。
When the concentration ratio of rice inhibitor with trypsin is 0.3, the trypsin of 50 percent can be suppressed the hydrolysing activity by matter L-BAPNA, and Semen Glycines inhibitor then needs the ratio of about 0.5 that the trypsin of 50 percent could be suppressed the same hydrolysing activity by matter.
This result of study shows for trypsin, and the inhibitor that the rejection ability of the inhibitor that rice bud scale is extracted is extracted than Semen sojae atricolor is preferred.It addition, also report trypsin inhibitor on other documents there is excellent anticancer effect.
Therefore, how to set up an environment and condition being suitable for cultivation rice bud scale, in order to cultivation rice bud scale, be the target of all circles' competitively effort, it addition, the extracting process of rice bud scale content, the direction that Yi Shi all circles are competitively studied.
Summary of the invention
The invention provides a kind of rice bud scale and the extracting process of content thereof and application, establish the environment and condition being suitable for cultivation rice bud scale, and utilize homogenizer to carry out rice bud scale to homogenize the method extracting content again.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of rice bud scale, is to be formed by following cultivation method:
A () rice disappearsPoisoningBacterium；
B () quiescent culture: changed water when rice bud scale starts to pip, stand the most always, is not required to change water, and completely cuts off air in this time of repose, make being grown under anaerobic environment of rice bud scale carry out；
(c) harvest rice bud scale.
Wherein it is preferred to, in described step (b), the temperature of quiescent culture is 4 DEG C～38 DEG C.
Wherein it is preferred to, in described step (b), the time of quiescent culture is 70～120 hours.
Wherein it is preferred to, when described step sends out harvest rice bud scale in (c), a length of the 4 of rice bud scale～10cm.
The present invention also provides the content extracting process of a kind of rice bud scale, comprises the following steps:
A () homogenizes: utilize a homogenizer to carry out rice bud scale and homogenize again with secondary deionized water extraction；
B () separates macromole and little molecule: utilizing concentrating and separating membrance separation to go out rice bud scale macromole and little molecule, the boundary line of described concentrating and separating membrance separation molecular weight is 5000KDa；
C () separates trypsin inhibitor: utilize cation exchange resin rough segmentation, by trypsin inhibitor and other hmw proteins and separation of small molecuies, obtains rice bud scale extract.
The present invention also provides the application in preparation has the health food of protective effect on cancer risk of the rice bud scale, the extract of described rice bud scale is added milled rice with embryo and is dried under low temperature, then clay into power, must have the health food of protective effect on cancer risk.
The present invention also provides the application in preparation has the cosmetics of free radical resisting and anti-aging effect of the rice bud scale.
The present invention also provides the application in preparation has the medicine of cell growth inhibiting rate action of the rice bud scale.
Rice bud scale extract application process of the present invention, it is in the culture medium containing 10% hyclone, adds the rice bud scale extract of 454ug/ml, has 37% significantly inhibit for the growth of HK-2cells.It addition, add the rice bud scale extract of 909.1ug/ml, then can suppress the cell growth rate of 27%.
The rice bud scale that anaerobic environment is cultivated, the clear liquor after water extracts there are about the free radical scavenging activity of 73%, and this rice bud scale extract has antioxidant activity.
Accompanying drawing explanation
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below by use required in embodiment or description of the prior artAccompanying drawingIt is briefly described, it should be apparent that, in describing belowAccompanying drawingIt is only some embodiments of the present invention, for those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to according to theseAccompanying drawingObtain otherAccompanying drawing。
Figure 1For the curve of sample antioxidation numerical value in Application ExampleFigure。
Detailed description of the invention
Below the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, broadly fall into the scope of protection of the invention.
Embodiment 1
The present invention provides a kind of rice bud scale, is to be formed by following cultivation method:
A () rice disappearsPoisoningBacterium, selects to obstruct No. 9 rice cultivated for rice bud scale with platform, cultivates front and disappearedPoisoningBacterium.
(b) quiescent culture, water is changed when rice bud scale starts to pip, stand the most always, this time of repose is not required to change water, and cultivation temperature system is 25 DEG C, and completely cut off air (covering isolation air with aluminium-foil paper) or add a little ethanol, make to carry out under being grown on anoxia or have the environment of ethanol of rice bud scale (, by anaerobic environment or add a little ethanol suppression the rice initial stage root growth (root growth when rice, leaf is emerged the most immediately, bud scale then cannot grow), in order to constitute an environment being beneficial to bud scale growth.
C () harvest bud scale, treats rice bud scale growth also quiescent culture 100 hours, when the growth of rice bud scale, more than 4～10cm length gets final product harvest, and the longest rice bud scale is less than 10cm.
Embodiment 2
The present invention provides a kind of rice bud scale, is to be formed by following cultivation method:
A () rice disappearsPoisoningBacterium, selects to obstruct No. 9 rice cultivated for rice bud scale with platform, cultivates front and disappearedPoisoningBacterium.
(b) quiescent culture, water is changed when rice bud scale starts to pip, stand the most always, this time of repose is not required to change water, and cultivation temperature system is 4 DEG C, and completely cut off air (covering isolation air with aluminium-foil paper) or add a little ethanol, make to carry out under being grown on anoxia or have the environment of ethanol of rice bud scale (, by anaerobic environment or add a little ethanol suppression the rice initial stage root growth (root growth when rice, leaf is emerged the most immediately, bud scale then cannot grow), in order to constitute an environment being beneficial to bud scale growth.
C () harvest bud scale, treats rice bud scale growth also quiescent culture 120 hours, when the growth of rice bud scale, more than 4～10cm length gets final product harvest, and the longest rice bud scale is less than 10cm.
Embodiment 3
The present invention provides a kind of rice bud scale, is to be formed by following cultivation method:
A () rice disappearsPoisoningBacterium, selects to obstruct No. 9 rice cultivated for rice bud scale with platform, cultivates front and disappearedPoisoningBacterium.
(b) quiescent culture, water is changed when rice bud scale starts to pip, stand the most always, this time of repose is not required to change water, and cultivation temperature system is 38 DEG C, and completely cut off air (covering isolation air with aluminium-foil paper) or add a little ethanol, make to carry out under being grown on anoxia or have the environment of ethanol of rice bud scale (, by anaerobic environment or add a little ethanol suppression the rice initial stage root growth (root growth when rice, leaf is emerged the most immediately, bud scale then cannot grow), in order to constitute an environment being beneficial to bud scale growth.
C () harvest bud scale, treats rice bud scale growth also quiescent culture 70 hours, when the growth of rice bud scale, more than 4～10cm length gets final product harvest, and the longest rice bud scale is less than 10cm.
Observe the growth record under anoxia and ventilatory conditions of the above-mentioned rice bud scale in the above-described embodiments, find in the case of anoxia, the speed of growth of rice bud scale, than very fast, is under anaerobic conditions cultivated 70 hours, and the length of rice bud scale is more than 4 centimeters, cultivate 70 hours when ventilation, the length of its rice bud scale the most about 2.1 centimeters, and find the most also there is root length out, therefore, under conditions of the cultivation optimal growth condition of rice bud scale, or setting anoxia most preferably.
Embodiment 4
The present embodiment provides the content extracting process of a kind of rice bud scale, comprises the following steps:
A () homogenizes: utilize a homogenizer to carry out rice bud scale and homogenize again with secondary deionized water extraction；
B () separates macromole and little molecule: utilizing concentrating and separating membrance separation to go out rice bud scale macromole and little molecule, the boundary line of described concentrating and separating membrance separation molecular weight is 5000KDa；Via experiment, macromole and little molecule, with 5000KDa as boundary line, are respectively provided with the effect of the suppression tryrosinase of 6.1% and 13.8%.
C () separates trypsin inhibitor: utilize cation exchange resin rough segmentation, by trypsin inhibitor and other hmw proteins and separation of small molecuies, obtains rice bud scale extract.
Detect by the carrying out of the west point method of the use of ink and water, we learn under anoxic conditions, the trypsin inhibitor that trypsin inhibitor major part is molecular weight 16KDa that bud scale is showed, along with the growth of blowing air time finds the trypsin inhibitor that another molecular weight is 25KDa, its oxygenation can show.
Embodiment 5 Application Example
Test the antioxidant activity of rice bud scale extract of the present invention: the rice cultivated in anaerobic environment, the clear liquor after water extracts there are about the free radical scavenging activity of 73%, and this rice bud scale extract has antioxidant activity.
Sample antioxidation numerical value is madeFigureRelativelySuch as figure 1Shown in.
Wherein, free clearance rate (scavenging activity, %)=[(A-B)/A] * 100%
A=blank group (blank) absorption value under 517nm
B=extract (sample) absorption value under 517nm
Embodiment 6 Application Example
Test the whitening active of rice bud scale extract of the present invention: find that the molecular weight rice bud scale extract more than 5000 only has the effect of 6.1% suppression tryrosinase, and molecular weight is less than the bud scale extract of 5000, has the effect of 13.8% suppression tryrosinase.
The whitening test result of bud scale composition:
UV light absorption value
| Arbutin | Vitamin C | Macromole | Little molecule | |
| At | 0.282 | 0.051 | 0.414 | 0.373 |
| Al | 0.013 | 0.049 | 0.039 | 0.029 |
| Ab | 0.400 | 0.400 | 0.400 | 0.4 |
| Ao | 0.001 | 0.001 | 0.001 | 0.001 |
Anti-melanin test result:
| Arbutin | Vitamin C | Macromole | Little molecule | |
| Suppression ratio (%) | 32.581 | 99.499 | 6.015 | 13.784 |
Suppression ratio (%)=(Ab-Ao)-(At-Al)/(Ab-Ao) * 100%
Embodiment 7 Application Example
Test the antioxidant activity of rice bud scale extract of the present invention: free radical DPPH under conditions of aerobic with anoxia is because being that 517nm has strong absorption value at wavelength, we utilize standard substance (Vit.E) and the sample of addition, follow the trail of under 517nm wavelength, the slippage of absorption value, directly proves that rice bud scale extract sample has non-oxidizability.
In antioxidant activity experiment test, free radical DPPH under conditions of aerobic with anoxia is because having strong absorption value at wavelength 517nm, and we utilize standard substance (Vit.E) and the sample of addition, follows the trail of under 517nm, the slippage of absorption value, directly proves that sample is stagnant to have non-oxidizability.
Utilize the method for antioxidative test in step, obtain absorption value such asFollowing table:
Embodiment 8 Application Example
The test bud scale extract Reproductive activity to cell: in the culture medium containing 10% hyclone, adds the bud scale extract of 454ug/ml, for the growth of HK-2cells, and have 37% significantly inhibit (p < 0.001).And add the bud scale extract of 909.1ug/ml, then can suppress the cell growth rate (p < 0.001) of 27%.This bud scale extract, in the case of containing hyclone, has the effect of suppression HK-2 cell growth rate really.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (8)
1. a rice bud scale, it is characterised in that be to be formed by following cultivation method:
(a) rice disinfection；
B () quiescent culture, is changed water when rice bud scale starts to pip, is stood the most always, should
It is not required to change water in time of repose, and completely cuts off air, make being grown under anaerobic environment of rice bud scale carry out；
(c) harvest rice bud scale.
A kind of rice bud scale the most according to claim 1, it is characterised in that: described step (b) stands
The temperature cultivated is 4 DEG C～38 DEG C.
A kind of rice bud scale the most according to claim 1, it is characterised in that: described step (b) stands
The time cultivated is 70～120 hours.
A kind of rice bud scale the most according to claim 1, it is characterised in that: described step is sent out in (c) and is received
When becoming rice bud scale, a length of the 4 of rice bud scale～10cm.
5. the content extracting process of the rice bud scale described in a claim 1, it is characterised in that include with
Lower step:
A () homogenizes: utilize a homogenizer to carry out rice bud scale and homogenize again with secondary deionized water extraction；
B () separates macromole and little molecule: utilize concentrating and separating membrance separation go out rice bud scale macromole with little point
Son, the boundary line of described concentrating and separating membrance separation molecular weight is 5000KDa；
(c) separate trypsin inhibitor: utilize cation exchange resin rough segmentation, by trypsin inhibitor with
Other hmw proteins and separation of small molecuies, obtain rice bud scale extract.
6. the answering in preparation has the health food of protective effect on cancer risk of the rice bud scale described in a claim 1
With, it is characterised in that: the extract of described rice bud scale is added milled rice with embryo and is dried under low temperature, then wear into
Powder, must have the health food of protective effect on cancer risk.
7. the rice bud scale described in a claim 1 has free radical resisting and the cosmetic of anti-aging effect in preparation
Application in product.
8. the rice bud scale described in a claim 1 has the medicine of cell growth inhibiting rate action in preparation
In application.
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| CN201610322824.XA CN105941115A (en) | 2016-05-13 | 2016-05-13 | Rice coleoptiles and content extraction method and application thereof |
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| CN201610322824.XA CN105941115A (en) | 2016-05-13 | 2016-05-13 | Rice coleoptiles and content extraction method and application thereof |
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