CN105923960A - Livestock excrement deodorant and deodorizing method and application thereof - Google Patents

Abstract

The invention discloses a livestock excrement deodorant and a deodorizing method and application thereof. By adding needle mushroom residues and horseradish powder, the concentration of phenols, indoles and volatile fatty acids in livestock excrement is reduced. Major odor constituents in indoles are reduced greatly. Reduction of phenols is caused by degradation of phenolic compounds, and degradation of phenolic compounds is caused by peroxidase in horseradish. The concentration of volatile fatty acids and branched chain fatty acids is affected obviously, volatile fatty acids in livestock excrement are reduced, and finally odor is relieved.

Description

A livestock manure deodorant and its deodorizing method and application

technical field

The invention relates to the technical field of environmental bioengineering, in particular to a livestock feces deodorant and its deodorizing method and application.

Background technique

In recent years, animal husbandry is one of the main causes of odor pollution. Epidemiological studies have shown that malodorous gases from livestock manure can affect the health of rural populations. At present, the dilemma of animal husbandry has attracted public attention, as well as the attention of legislative and environmental management departments. For the sustainability of livestock production, the management method of reducing the emission of malodorous gas is a method with good application potential at present. Odor generation is a complex process related to the production of volatile compounds by bacteria during storage of pig manure.

Pig manure contains both incompletely digested nutrients and an unpleasant smell. Anaerobic fermentation of microorganisms in pig manure storage pits is the main cause of odor. Studies have shown that there are about 200 kinds of odor compounds, including phenols, indoles, volatile fatty acids, sulfides and ammonia in livestock manure. Phenols and indoles are essential for odor generation due to their low odor threshold concentrations. Fermentation of undigested organic compounds in pig manure produces odorous compounds. The concentration of branched-chain fatty acids and aromatic compounds in pig manure increased when protein components were added in excess, while volatile fatty acids increased when carbohydrates were added. When pigs are fed a protein-rich diet, the ammonia nitrogen concentration in pig manure increases.

Some studies on the control of pig manure odor have shown that reducing pig dietary crude protein can reduce malodorous compounds, and controlling dietary additives can reduce fecal odor compounds. At present, in order to reduce the generation of odor, many studies pay attention to the concentration detection of odor. The analysis of gaseous compounds, the control of precursor compounds that produce odor, and the development of effective biological control systems are of great significance to the control of odorous substances.

Contents of the invention

In order to overcome the defects of the prior art, the invention provides a livestock manure deodorant and its deodorizing method and application.

The technical solution adopted in the present invention is: a livestock manure deodorant, the deodorant is a solution containing 0.5-1.5% Flammulina velutipes slag.

The deodorant is an aqueous solution containing 1% Flammulina velutipes slag.

The deodorant is an aqueous solution containing 0.5-1.5% Flammulina velutipes residue, 0.015-0.045% horseradish powder and 112 ml 3% hydrogen peroxide.

The deodorant is an aqueous solution containing 1% Flammulina velutipes slag, 0.03% horseradish powder and 112 ml 3% hydrogen peroxide.

A deodorization method of the described livestock manure deodorant, comprising the steps of: spraying on the livestock manure comprising an aqueous solution containing concentration of 1% Flammulina velutipes residue, 0.03% horseradish powder and 112 ml of 3% hydrogen peroxide, and treating 10-15 days later.

An application of the livestock manure deodorant to deodorize livestock manure, urine, mixture of manure and urine, and compost.

The beneficial effects of the present invention are: the present invention provides a livestock manure deodorant and its deodorizing method and application, by adding Flammulina velutipes residue and horseradish powder, the phenols, indoles and volatile fatty acids in livestock manure are reduced concentration. The important odor components in indoles are greatly reduced. The reduction of phenols is caused by the degradation of phenolic compounds, and the degradation of phenolic compounds is caused by peroxidase in horseradish. The present invention significantly affects volatile fatty acids With the concentration of branched chain fatty acids, the volatile fatty acids in livestock manure are reduced, which ultimately reduces the odor.

detailed description

Example 1

Sample Collection:

Pigs were fed a basal diet (digestible energy, 3450 kcal/kg; crude protein, 16.5%; total lysine, 0.87%). Three kinds of additive treatment conditions were set and repeated 4 times. The treatment groups included 0.03% aqueous solution of horseradish powder + 112 ml 3% hydrogen peroxide solution, 1% aqueous solution of enoki mushroom residue and 1% aqueous solution of enoki mushroom residue + 0.03% aqueous solution of horseradish powder + 112 ml 3 % hydrogen peroxide solution. Pig manure was collected from a pigsty pit, and after additive treatment, the pig manure mixture was placed in a 20-L test chamber (similar to a pigsty pit), and incubated at 20°C for 14 days with continuous ventilation (15 ml/min). Because in southern Zhejiang, the average temperature is about 20°C from April to October, when the pig manure is placed on the pig pen pit for about 2 weeks, the smell problem in the pig farm is the most serious. Samples were collected from a depth of 10 cm below the surface of pig manure in the test box and stored at -20°C for later use.

Example 2

Sample Collection:

Pigs were fed a basal diet (digestible energy, 3450 kcal/kg; crude protein, 16.5%; total lysine, 0.87%). Three treatment conditions were set and repeated 4 times. The treatment groups included 0.015% aqueous solution of horseradish powder + 112 ml 3% hydrogen peroxide solution, 1.5% aqueous solution of enoki mushroom residue, and 1.5% aqueous solution of enoki mushroom residue + 0.015% aqueous solution of horseradish powder + 112 ml 3% hydrogen peroxide solution. Pig manure was collected from a pigsty pit, and after being treated with additives, the pig manure mixture was placed in a 20-L test chamber (similar to a pigsty pit), and incubated at 20°C for 14 days with continuous ventilation (15 ml/min). Because in southern Zhejiang, the average temperature is about 20°C from April to October, when the pig manure is placed on the pig pen pit for about 2 weeks, the smell problem in the pig farm is the most serious. Samples were collected from a depth of 10 cm below the surface of pig manure in the test box and stored at -20°C for later use.

Example 3

Sample Collection:

Pigs were fed a basal diet (digestible energy, 3450 kcal/kg; crude protein, 16.5%; total lysine, 0.87%). Three treatment conditions were set and repeated 4 times. The treatment groups included 0.045% aqueous solution of horseradish powder + 112 ml 3% hydrogen peroxide solution, 0.5% aqueous solution of enoki mushroom slag, and 0.5% aqueous solution of enoki mushroom slag + 0.045% aqueous solution of horseradish powder + 112 ml 3% hydrogen peroxide solution. Pig manure was collected from a pigsty pit, and after additive treatment, the pig manure mixture was placed in a 20-L test chamber (similar to a pigsty pit), and incubated at 20°C for 14 days with continuous ventilation (15 ml/min). Because in southern Zhejiang, the average temperature is about 20°C from April to October, when the pig manure is placed on the pig pen pit for about 2 weeks, the smell problem in the pig farm is the most serious. Samples were collected from a depth of 10 cm below the surface of pig manure in the test box and stored at -20°C for later use.

Composition Analysis of Odor Compounds

The composition of the odorous substance was determined by a gas chromatograph (6890N, Agilent Technologies) equipped with a flame ionization detector. The analysis of phenols and indoles was carried out on a DB-5 column (30 m × 0.25 mm × 0.25 μm, Agilent Technologies). Gas chromatography analysis parameters are as follows: separation mode, 5:1; inlet and detector temperature, 250°C; injection volume, 2 μl. The furnace temperature program was as follows: initial temperature of 50°C for 5 min; 10°C/min to 220°C for 2 min. Volatile fatty acids were extracted with 25% HPO3 solution (Sigma-Aldrich) and analyzed on an HP-INNOWax column (30 m × 0.25 mm × 0.25 μm, Agilent Technologies) under the following conditions: separation mode, 10:1; inlet and Detector temperature, 250°C; injection volume, 0.2 μl. The furnace temperature program was as follows: initial temperature of 80 °C for 5 min, 20 °C/min to 120 °C for 0 min, 10 °C/min to final temperature of 205 °C for 2 min.

Analysis of odorous compounds

After adding horseradish powder and Flammulina velutipes residue to treat pig manure for 14 days, the concentrations of phenols, indoles and volatile fatty acids in pig manure were detected. After treatment with horseradish powder, the concentration of phenol decreased by 9%, and the concentration of p-cresol (one of the important odor components in phenols) decreased by 10%. After treatment with Flammulina velutipes slag, the concentration of indole decreased by 25%, and the concentration of methyl indole (one of the important odor components in indoles) decreased by 32% (Table 1). The reduction of phenolics was caused by the degradation of phenolic compounds caused by peroxidase in horseradish.

The reduction of methyl indole concentration was caused by the addition of Flammulina velutipes residue, which may be due to the relative decrease of protein degradation due to the increase of the proportion of carbohydrates in pig manure. In addition, the short-chain fatty acids in pig manure from Flammulina velutipes significantly increased (Table 1). The polysaccharides of Flammulina velutipes residue include hemicellulose, cellulose and lignin, all of which are easy to ferment. Since fermentable carbohydrates increase the formation of volatile fatty acids and reduce the growth of methylindole-producing bacteria, resulting in a decrease in the level of methylindole in pig manure, pig manure with horseradish powder (pH 7.27) and control Group (pH 7.36), the pH (7.01) of Flammulina velutipes slag pig manure was lower. Adding fermentable carbohydrates to pig manure according to the present invention reduces malodorous odor (Table 1).

The horseradish powder treatment group showed a significant effect on the concentration of volatile fatty acids and branched chain fatty acids, with a reduction of 24% and 33%, respectively (Table 1). Peroxidase reacts with peroxygen compounds to generate dissolved oxygen and activate aerobic bacteria in pig manure. These activated aerobic bacteria are able to break down malodorous compounds and reduce volatile fatty acids in pig manure.

The above descriptions are only preferred implementations of the present invention, and the scope of protection of the present invention is not limited to the above-mentioned embodiments, and all technical solutions under the idea of the present invention belong to the scope of protection of the present invention. It should be pointed out that for those skilled in the art, some improvements and modifications without departing from the principles of the present invention should also be regarded as the protection scope of the present invention.

Claims (6)

1. an excrement of animals deodorizer, it is characterised in that described deodorizer is for including that containing concentration be 0.5-1.5% acupuncture needle The solution of mushroom slag.

A kind of excrement of animals deodorizer the most according to claim 1, it is characterised in that described deodorizer for include containing Concentration is the aqueous solution of 1% Flammulina velutiper (Fr.) Sing slag.

A kind of excrement of animals deodorizer the most according to claim 1, it is characterised in that described deodorizer for include containing Concentration be 0.5-1.5% Flammulina velutiper (Fr.) Sing slag, concentration be 0.015-0.045% horseradish powder and the aqueous solution of 112 ml 3% hydrogen peroxide.

A kind of excrement of animals deodorizer the most according to claim 3, it is characterised in that described deodorizer for include containing Concentration be 1% Flammulina velutiper (Fr.) Sing slag, concentration be 0.03% horseradish powder and the aqueous solution of 112 ml 3% hydrogen peroxide.

5. the deodorizing method of the excrement of animals deodorizer described in a claim 1, it is characterised in that comprise the following steps: Spray on excrement of animals include containing concentration be 1% Flammulina velutiper (Fr.) Sing slag, concentration be 0.03% horseradish powder and 112 ml 3% hydrogen peroxide Aqueous solution, after processing 10-15 days.

6. the excrement of animals deodorizer described in a claim 1 is on feces of livestock and poultry, urine, slurry of feces and urine, compost deodorizing Application.