CN105886614B - A kind of identification method and its primer special of upland cotton Asia chicken foot leaf germplasm materials - Google Patents
A kind of identification method and its primer special of upland cotton Asia chicken foot leaf germplasm materials Download PDFInfo
- Publication number
- CN105886614B CN105886614B CN201610246829.9A CN201610246829A CN105886614B CN 105886614 B CN105886614 B CN 105886614B CN 201610246829 A CN201610246829 A CN 201610246829A CN 105886614 B CN105886614 B CN 105886614B
- Authority
- CN
- China
- Prior art keywords
- chicken foot
- sub
- leaf
- foot leaf
- asia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 59
- 239000000463 material Substances 0.000 title claims abstract description 35
- 244000299507 Gossypium hirsutum Species 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 23
- 235000009429 Gossypium barbadense Nutrition 0.000 title claims abstract description 18
- 235000018322 upland cotton Nutrition 0.000 title claims abstract description 18
- 238000012408 PCR amplification Methods 0.000 claims abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 229920003266 Leaf® Polymers 0.000 description 67
- 241000196324 Embryophyta Species 0.000 description 23
- 229920000742 Cotton Polymers 0.000 description 14
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 238000000926 separation method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 238000000137 annealing Methods 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000002837 defoliant Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000007380 fibre production Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides the identification method and its primer special of a kind of upland cotton Asia chicken foot leaf germplasm materials, and the sub- chicken foot leaf character that all to upland cotton Asia chicken foot leaf germplasm materials can have carries out early stage Rapid identification, accelerates breeding process.Using the genomic DNA of upland cotton to be measured as template, PCR amplification is carried out using specific primer, is judged according to the banding pattern that PCR product is presented on gel, containing only 230bp banding pattern is sub- chicken foot leaf homozygote, it is normal broad-leaved containing 200bp banding pattern, the two all has plenty of sub- chicken foot leaf heterozygote.The present invention can indoors identify sub- chicken foot leaf germplasm materials purity with seed or seedling, accelerate breeding process, improve the efficiency of selection of excellent sub- chicken foot leaf homozygosis material.
Description
Technical field
The present invention relates to the identification methods and its primer special of a kind of upland cotton Asia chicken foot leaf germplasm materials, belong to cotton point
Sub- biological applications field.
Background technique
Cotton is the important industrial crops in China, and major product cotton fiber is important textile industry raw material, is produced in weaving
The effect that can not be substituted is played in industry.Blade is the photosynthetic organs that heavy cotton is wanted, and is responsible for luminous energy being converted to carbohydrate
For plant growth and development.The variation of blade shape has a significant impact to the growth and development of cotton, and then influences cotton fiber production
Amount and quality.Upland cotton is leaf to be divided into normal leaf and drastic crack leaf.It is duck's foot type that normal leaf is leaf, and leaf splits shallower, and leaf area is big,
Mismanagement is typically easy to cause Cotton Canopy is strongly fragrant to cover, and so that middle and lower part cotton boll is generated severe detachment because of illumination deficiency, overcast and rainy year
Part be easy to cause rotten bell, is easy to influence the spraying effect of defoliant, influences machine pick cotton quality.
Sub- chicken foot leaf belongs to cotton drastic crack leaf, and leaf is split relatively deeply, and leaf-area coefficient is moderate；It is being effectively improved Architecture of Cotton Canopy
While, the problem of chicken foot leaf cotton Efficient leaf area deficiency can be made up to a certain extent；In addition, sub- chicken foot leaf phenotype can be used as
Morphological markers have larger purposes in cotton hybrid vigor utilization for distinguishing true and false hybrid.
Currently, generally identifying sub- chicken foot leaf material by investigating leaf phenotype in flower bud phase and florescence in production.To sub- chicken
The identification of foot leaf homozygosis material and hybrid material is usually required preferred material kind at plant, in leaf point of next season plant
It can determine whether the single plant is homozygous type from phenotypic evaluation rear.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of identification method of upland cotton Asia chicken foot leaf germplasm materials and its
Primer special, the sub- chicken foot leaf character that all to upland cotton Asia chicken foot leaf germplasm materials can have carry out early stage Rapid identification, add
Fast breeding process.
Therefore, the technical solution adopted by the present invention is as follows: a kind of identification method of upland cotton Asia chicken foot leaf germplasm materials, with
The genomic DNA of upland cotton to be measured be template, using primer pair: forward primer 5 '-GATGCACCAGATCCTTTTAT -3 ' and
Reverse primer 5 '-GGTACATCGGAATCACAGT -3 ' carries out PCR amplification, the banding pattern presented on gel according to PCR product
Judged, it is normal broad-leaved containing 200bp banding pattern that containing only 230bp banding pattern, which is sub- chicken foot leaf homozygote, and the two has
It is sub- chicken foot leaf heterozygote.
Above-mentioned banding pattern is master tape banding pattern.
The pcr amplification reaction system and program are as follows:
PCR system is 20ul, includes 2 × EasyTaqPCR Supermix 10ul, DNA profiling 1ul, the forward primer
With each 1ul of reverse primer, DDH2O 7ul；
PCR program is 94 DEG C of initial denaturation 5min, 94 DEG C of denaturation 30s, 55 DEG C of annealing 30s, 72 DEG C of extension 50s (35 recycle)
Extend 10min after 72 DEG C；
Another object of the present invention is to provide the primer in preparation for identifying or assisting identification upland cotton Asia chicken foot leaf kind
Application in the kit of material.
The present invention also provides a kind of for identifying or assisting the kit of identification upland cotton Asia chicken foot leaf germplasm materials, contains
The primer.
The existing method identified sub- chicken foot leaf material has the following disadvantages:
1. not being that too obviously, can only identify, can not sieve since flower bud phase since leaf splits phenotype in cotton fertility performance early period
Select the sub- chicken foot leaf single plant that wheat seeding is excellent.
It is easily protected from environmental 2. leaf phenotype belongs to morphological markers, brings certain difficulty to the stability of phenotypic evaluation.
3. from hereditary angle, sub- chicken foot leaf is incomplete dominant lnheritance to normal leaf, but in crop field breeding process
Middle homozygous sub- chicken foot leaf is homozygous larger with the differentiation difficulty of heterozygous, affects the screening effect of excellent homozygous sub- chicken foot leaf material
Rate affects breeding process.
Therefore, for above-mentioned prior art the shortcomings that, the present invention have selected the sub- chicken foot leaf material of based on PCR molecular labeling
Screening technique, the method for the present invention can in advance identify sub- chicken foot leaf material, and ensure sub- chicken foot leaf material screening simultaneously
Accuracy and high efficiency.
Compared with prior art the invention has the following advantages that
1. can be identified indoors with seed or seedling sub- chicken foot leaf germplasm materials purity by the invention, big
The identification period of sub- chicken foot leaf material can be advanceed to seedling stage by Tanaka, filter out the sub- chicken foot leaf material being excellent in seedling stage；
2. the DNA molecular marker that the present invention uses belongs to neutral label, do not influenced by period, environment and histoorgan,
Qualification result is reliable and stable；
3. the present invention can distinguish sub- chicken foot leaf homozygote and heterozygote material, convenient for accelerating breeding process, excellent Asia is improved
The efficiency of selection of chicken foot leaf homozygosis material.
Detailed description of the invention
Fig. 1 is the form phenotype and marker genetype Statistical Comparison of part single plant.
Specific embodiment
Technical solution of the present invention and its generated technical effect are carried out below with reference to specific test method further
Elaboration, the following description is only intended to explain the invention, but the present invention is limited in any way, based on the present invention religion
It is any made by leading to transform or replace, it all belongs to the scope of protection of the present invention.
Method used in the present invention is conventional method in that art unless otherwise specified.
Test material as used in the following examples, reagent etc., are commercially available unless otherwise specified.Institute
Each water article kind (strain) used is conventional use of kind (strain) in breeding field, by country authorization assert or
Technical appraisement, can be in species bank acquisition or market purchasing.
A kind of identification method of upland cotton Asia chicken foot leaf germplasm materials of one, of embodiment, steps are as follows:
1. seed or seedling stage take upland cotton fresh material tender leaf, genomic DNA is extracted, and detect to DNA mass,
It is diluted to 50ng/ul；
2. according to known cotton EST series, a pair of special SSR label primer of SSRhunter software design, forward primer:
5'—GATGCACCAGATCCTTTTAT—3'(SEQ ID No.1)；Reverse primer: 5 '-GGTACATCGGAATCACAGT-
3’(SEQ ID No.2)。
The est sequence of design primer is following (SEQ ID No.3), and underscore indicates primer location.
CTGGACTAACACCAATAATCACTAAACTTTGATTAAAATAACATTTCAGTTACTAAACTTTCAAAAGTG
ACAAATCAGTCATTAACATTTACGAAAAGTGACAAATTAGTCACCTGAGAGTGGACATCTGACGTGGCCCGTTAGGG
TGCCACGTTGAACATGATCGGATGCACCAGATCCTTTTATTAGTTTATAAGGATTACCAACTAAATAGAAGAAGAAG
AAGAAGAGGAAGAAGAAGAAGAAAAGGAAGAAGAAGAAGAAGAATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAA
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAAAGAGAAATGGAAATA
CCCACTGTGATTCCGATGTACCGTCATTTGGACGGAATTTAAAAGACACTTTAGTTGTTATTAAGAACTGATTTTTA
TTTGGATTTAAAAGAC
3. carrying out PCR amplification using diluted DNA as template, reaction system and operation program are as follows:
PCR system is 20ul, includes 2 × EasyTaqPCR Supermix 10ul, DNA profiling 1ul, upstream primer is under
Swim each 1ul of primer, DDH2O 7ul；
PCR program is 94 DEG C of initial denaturation 5min, 94 DEG C of denaturation 30s, 55 DEG C of annealing 30s, 72 DEG C of extension 50s (35 recycle)
Extend 10min after 72 DEG C；
4.PCR product is separated by electrophoresis in 8% PAGE glue, and voltage 120V, 2h are dyed using argentation；
5. marker genetype is identified, is judged according to the banding pattern that PCR product is presented on gel, contain only 230bp band
Type is sub- chicken foot leaf homozygote, is normal broad-leaved containing 200bp banding pattern, and the two all has plenty of sub- chicken foot leaf heterozygote.
Two, of embodiment carries out the identification of sub- chicken foot leaf with the method for the present invention to 100 samples
1. material:
No. 28 are ground as female parent with normal broad-leaved material Shandong cotton, and sub- chicken foot leaf homozygosis material S131189 system is male parent, building packet
Contain the sub- chicken foot leaf and normal broad-leaved separation F of 229 single plants2Group.
2.DNA is extracted:
100 single plants are randomly selected in seedling stage, extract young leaflet tablet, extract genomic DNA with CTAB method, and to DNA matter
Amount is detected, and concentration is adjusted to 50ng/ul.
3.PCR reaction:
PCR reaction carries out on ABI9700PCR instrument, system 20ul, 2 × EasyPCR mix including 10ul, 1ul's
DNA profiling, upstream primer and downstream primer each 1ul, the DDH of 7ul2O.Response procedures are 94 DEG C of initial denaturation 5min, 94 DEG C of denaturation
30s, 55 DEG C of annealing 30s, 72 DEG C of extension 50s, 35 recycle, 72 DEG C of extension 10min.
4. electrophoresis and genotype identification:
PCR product is separated by electrophoresis on 8% polyacrylamide gel, as shown in Figure 1, statistic mass 230bp (Brazil
014 genotype) and 200bp (S131189) banding pattern.Only 230bp banding pattern genotype is denoted as B；Only 200bp banding pattern genotype is
A；H is denoted as comprising two kinds of 230bp and 200bp banding patterns simultaneously.
5. leaf phenotypic evaluation:
It is identified respectively at flower bud phase and seedling stage the separation phenotype leaf to group.Normal broad-leaved is labeled as B, sub- chicken foot leaf
Homozygosis label is that sub- chicken foot leaf hybrid marker is H.
6. data statistics:
The form phenotype and genotype of segregating population are counted respectively, and check the identical situation of genotype and phenotype.
7. result and analysis:
The statistics display of form phenotype, in 100 single plants, 23 plants of normal broad-leaved, sub- chicken foot leaf is 32 plants homozygous, sub- chicken foot leaf
45 plants of heterozygosis.Genotype statistics shows that in 100 individuals, the individual that genotype is A is 40, and gene is the individual 36 of H, gene
Type is individual 23 of B.The phenotype of normal broad-leaved is consistent with genotypic expression, the phenotype and gene of sub- chicken foot leaf homozygosis and heterozygosis
Type has 8 single plant performance differences, and the Phenotypic Expression that 6 genotype are A is sub- chicken foot leaf heterozygosis, the phenotype table that 2 genotype are H
It is now homozygous for sub- chicken foot leaf.By 100 single plant kinds at plant, the coincide plant phenotype of single plant of 92 genotype and phenotype does not occur
Separation, the plant for the single plant that 6 genotype are A do not occur all sub- chicken foot leafs of leaf separation, illustrate that 6 single plants are all
Sub- chicken foot leaf homozygosis single plant.2 genotype are H, and phenotypic evaluation is the single plant of A, all sub- chicken foot leafs of the plant of 1 single plant,
Other 1 shows as sub- chicken foot leaf and normal leaf separation.
By comparing it is known that the accuracy rate that routine phenotypic is identified is 92%, and genotype identification of the present invention is accurate
Rate is 99%；And routine phenotypic identification can only be identified since flower bud phase, can not screen the excellent sub- chicken foot leaf single plant of wheat seeding,
And genotype identification of the present invention can advance to seedling stage, filter out the sub- chicken foot leaf material being excellent in seedling stage.This illustrates this hair
It is bright more more reliable than Morphological Identification based on the identification method of molecular labeling.
Claims (2)
1. a kind of identification method of upland cotton Asia chicken foot leaf germplasm materials, characterized in that the genomic DNA with upland cotton to be measured is
Template, using primer pair: forward primer 5 '-GATGCACCAGATCCTTTTAT -3 ' and reverse primer 5 ' -
GGTACATCGGAATCACAGT -3 ' carries out PCR amplification, is judged according to the banding pattern that PCR product is presented on gel, only
It is sub- chicken foot leaf homozygote containing 230bp banding pattern, is normal broad-leaved containing 200bp banding pattern, the two some is sub- chicken feet
Leaf heterozygote.
2. a kind of for identifying or assisting the kit of identification upland cotton Asia chicken foot leaf germplasm materials, characterized in that containing having the right
It is required that 1 primer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610246829.9A CN105886614B (en) | 2016-04-20 | 2016-04-20 | A kind of identification method and its primer special of upland cotton Asia chicken foot leaf germplasm materials |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610246829.9A CN105886614B (en) | 2016-04-20 | 2016-04-20 | A kind of identification method and its primer special of upland cotton Asia chicken foot leaf germplasm materials |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105886614A CN105886614A (en) | 2016-08-24 |
| CN105886614B true CN105886614B (en) | 2019-05-24 |
Family
ID=56704121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610246829.9A Active CN105886614B (en) | 2016-04-20 | 2016-04-20 | A kind of identification method and its primer special of upland cotton Asia chicken foot leaf germplasm materials |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105886614B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103757030A (en) * | 2014-01-14 | 2014-04-30 | 浙江农林大学 | Alleles for regulating and controlling two types of main stem table fur types of upland cotton, and single stranded conformational polymorphism (SSCP) molecular marker for identification thereof |
| WO2014172529A1 (en) * | 2013-04-17 | 2014-10-23 | Pioneer Hi-Bred International, Inc. | Methods for characterizing dna sequence composition in a genome |
-
2016
- 2016-04-20 CN CN201610246829.9A patent/CN105886614B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014172529A1 (en) * | 2013-04-17 | 2014-10-23 | Pioneer Hi-Bred International, Inc. | Methods for characterizing dna sequence composition in a genome |
| CN103757030A (en) * | 2014-01-14 | 2014-04-30 | 浙江农林大学 | Alleles for regulating and controlling two types of main stem table fur types of upland cotton, and single stranded conformational polymorphism (SSCP) molecular marker for identification thereof |
Non-Patent Citations (2)
| Title |
|---|
| Mapping and genomic targeting of the major leaf shape gene (L) in Upland cotton (Gossypium hirsutum L.);Andres RJ et al.;《Theoretical and Applied Genetics》;20140131;第127卷(第1期);摘要 |
| 陆地棉张氏鸡脚叶标记系的鉴定与比较;韩世杰等;《棉花科学》;20160229;第38卷(第1期);第11-14页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105886614A (en) | 2016-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112005878B (en) | Method for rapidly breeding corn haploid induction line and application thereof | |
| CN107201404A (en) | A kind of molecular biology identification method and its application for Asparagus dioecian plant sex | |
| CN106755480A (en) | A kind of SSR molecular marker I for identifying Gala apple Progeny plants and its application | |
| CN109251995B (en) | CAPS molecular marker for identifying watermelon peel background color and application thereof | |
| CN108384875A (en) | A kind of and the relevant insertion/deletion site of capsicum mellow fruit color gene, molecular labeling, molecular labeling primer and application | |
| CN112391489B (en) | SNP molecular marker related to watermelon flesh color and application thereof | |
| CN109536638A (en) | The molecular labeling and its application that a kind of and rape pattern character is closely related | |
| Del et al. | Marker-assisted introgression of a broad-spectrum resistance gene, Pi40 improved blast resistance of two elite rice (Oryza sativa L.) cultivars of Turkey | |
| CN104928396A (en) | Method for rapidly identifying hot pepper species and golden pepper purity degree by using EST-SSR molecular markers | |
| Rawat et al. | Comparative assessment of SSR, ISSR and AFLP markers for characterization of selected genotypes of Himalayan Chir pine (Pinus roxburghii Sarg.) based on resin yield | |
| CN106350591A (en) | SSR primers for establishing colored-leaf goldenrain tree variety fingerprint spectrum, established fingerprint spectrum and application thereof | |
| CN102766625B (en) | Molecular marker of rice major gene bph22 (t) resistant to brown planthoppers and application thereof | |
| CN105886614B (en) | A kind of identification method and its primer special of upland cotton Asia chicken foot leaf germplasm materials | |
| CN106801105A (en) | The SSR marker being associated with ramee fineness and application | |
| CN111349717A (en) | SSR (simple sequence repeat) markers of sweet cherry stock resources and fingerprint spectrum database thereof | |
| KR101444178B1 (en) | A method for identifying hot pepper varieties using microsatellites markers | |
| Reflinur et al. | The potential use of SSR markers to support the morphological identification of Indonesian mungbean varieties | |
| CN108977573A (en) | A method of seven-star radish purity of hybrid is identified using SSR molecular marker | |
| CN109234447A (en) | A kind of method for identifying No. 4 microspecies soybean resources of anti-soybean cyst nematode Heterodera glycines and dedicated SSR primer | |
| CN108531636A (en) | A kind of molecular marked compound TJcM01 and its application for identifying muskmelon unisexual flower | |
| CN105420354B (en) | Conventional rice variety Huaihe River rice No. 5 and Huaihe River rice No. 18 identification methods based on InDel label | |
| Ancora et al. | The first three clones selected from the traditional artichoke ‘Romanesco’populations and proposed for the release of new varieties | |
| CN104560961A (en) | Molecular markers linked to ZYMV (zucchini yellow mosaic virus-1) dominant disease-resistant gene ZYMV-1 and application of molecular markers | |
| CN114231656A (en) | SSR core primer group for identifying purity of cauliflower hybrid and screening method and application thereof | |
| Juliano et al. | Genetic differentiation in Oryza meridionalis Ng based on molecular and crossability analyses |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |