CN105886583A - Bovine bone marrow peptide extraction method - Google Patents
Bovine bone marrow peptide extraction method Download PDFInfo
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- CN105886583A CN105886583A CN201510042296.8A CN201510042296A CN105886583A CN 105886583 A CN105886583 A CN 105886583A CN 201510042296 A CN201510042296 A CN 201510042296A CN 105886583 A CN105886583 A CN 105886583A
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- 238000001914 filtration Methods 0.000 claims abstract description 21
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- 238000010792 warming Methods 0.000 claims description 12
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to a bovine bone marrow peptide extraction method, which comprises steps of cleaning and breaking, digesting, discharging soup, hydrolyzing, filtering, concentrating and spray-drying. With the application of the extraction method disclosed by the invention, problems on the effective absorption and utilization of macromolecular protein in human body are effectively solved, and micromolecular transformation and extraction of the protein are achieved. Bovine bone marrow peptide, as a micromolecular peptide substance extracted from bovine bone marrow through biological enzymolysis, is extra-strong in activity of an essence ingredient, and the essence ingredient is quite easy for human absorption, so that human immunity can be comprehensively regulated. The bovine bone marrow peptide disclosed by the invention, as a quite clear nutrient substance, is free from chemical ingredients and hormones and is free from toxic and side effects to human body; the bovine bone marrow peptide can rapidly dissolve in water without stirring; a water solution of the bovine bone marrow peptide is represented as a clear and transparent liquid in white or light yellow, and the water solution is free from the precipitation of impurities or precipitates; and the bovine bone marrow peptide plays an important role in such physiological activities as human's growth, development, metabolism, reproduction and the like.
Description
Technical field
The present invention relates to bioengineering field, particularly to a kind of method extracting small-molecular peptides from ox bone marrow.
Background technology
Containing a lot of nutrition maintained required for human life in ox bone marrow, wherein there is the indispensable phosphorus of human brain
Lipid, phosphoprotein etc., also prevent the ossein of human senescence, chondroitin, promote liver function methionine,
Each amino acid, vitamin A, B1, B2 and other trace element.
Product with ox bone marrow as raw material is few the most on the market, substantially eats to directly heat, and straight
Connect and also taken in substantial amounts of lipoid material while eating, original protein can not be changed in itself
Macromolecular structure, be difficult to be absorbed and utilized by the body.
Summary of the invention
In order to solve how prior art allows high molecular weight protein how effectively be absorbed by human body and asking of utilizing
Topic, carries out conversion and the extraction of little molecule to albumen, and the present invention provides a kind of ox bone marrow peptide extracting method.Institute
State technical scheme as follows:
A kind of ox bone marrow peptide extracting method, comprises the following steps:
Clean destruction step, by ox bone according to set clean duration be carried out, will clean after ox bone according to
Set broken size to crush；
Boiling step, puts into steaming plant by water, puts into the meter described ox bone of weight, and same stepping while intensification
Row stirring, stops heating up after being warming up to set heat-preserving range, and is incubated according to setting insulation duration, protects
Temperature stops stirring after terminating, and stands then according to set standing duration, forms a soup；
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storage device；
Hydrolysing step, pumps in hydrolysis device by a soup in a described soup storage device, is warming up to set
Determining sterilising temp and keep according to setting sterilizing duration, being then cooled to set hydrolysis temperature, addition is joined
Put liquid by a soup pH value regulation to neutral, put into animal protein enzyme preparation and be hydrolyzed, and keep to setting
Enzymolysis duration, enzymolysis is warming up to set enzyme-removal temperature, and keeps to setting the enzyme duration that goes out after terminating, formation is gone out
Hydrolyzate after enzyme；
Filtration step, stands after the hydrolyzate after the described enzyme that goes out puts into diatomite and/or activated carbon, and utilizes
Filter filters to clear liquid storage device, then utilizes circulation filter to follow according to setting described clear liquid
Ring filters duration and is circulated filtration；
Concentration step, is concentrated into the clear liquid after described circulating filtration setting and concentrates concentration, after forming clarification
Concentrate；
Spray drying step, by the concentrate after described clarification according to setting EAT and setting leaving air temp
It is spray-dried, forms ox bone marrow peptide pulvis.
Cleaning in destruction step, is setting cleaning duration scope as 20～40 minutes, set and crush size range
It it is 20～40 centimetres.
In boiling step, set heat-preserving range as 80～100 DEG C, set insulation duration scope as 8～12 hours,
Set standing duration scope as 0.5～1.5 hour.
In row's soup step, draw-out device is specially vacuum extraction tank.
In hydrolysing step, set sterilising temp scope as 90～110 DEG C, set sterilizing duration scope as 20～40
Minute, setting hydrolysis temperature scope as 55～75 DEG C, configuration liquid is specially NaoH, sets enzymolysis duration scope
It is 4～8 hours, sets enzyme-removal temperature scope as 80～100 DEG C, set enzyme duration scope of going out as 20～40 minutes.
In filtration step, filter is specially tub filter and/or two-stage plate filter, is circulated throughout
Filter device is specially plate filter, sets circulating filtration duration scope as 1～3 hour.
In concentration step, set the concentration concentration range concentrate need profit again as 10～20 Baumes, to muddiness
Carry out filtering or utilize tube centrifuge to separate with plate filter, the concentrate of described muddiness is formed
The concentrate of clarification.
In spray drying step, set EAT scope as 190～210 DEG C, set leaving air temp scope as
90-100℃。
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and utilize, to albumen
Carry out conversion and the extraction of little molecule.The ox bone marrow peptide of the present invention be that ox bone marrow is carried out biological enzymolysis extraction and
Becoming small-molecule peptide material, the essential ingredient activity in ox bone marrow peptide is superpower, is easily absorbed by the body, can be complete
Face regulation body immunity.The present invention does not contains any chemical composition and hormone, does not has the secondary work of any poison to human body
With, it is the purest nutriment.Can be dissolved in rapidly water without stirring, the aqueous solution is limpid transparent, for
White or light yellow transparent liquid, will not separate out any impurity and sediment.To the growth of human body, growth,
The physiological activity such as metabolism, procreation has vital effect.
Ox bone marrow peptide molecular weight, all below 1000 dalton, has the high property digested and assimilated and utilization rate.
It directly can be absorbed by the body without gastro-intestinal digestion.Can effectively ensure that protein is supplemented by human body.
Ox bone marrow peptide activity is strong, is easily absorbed by the body, fully adjustable body immunity.Function assessment test shows,
Ox bone marrow Toplink adjusts human body TH/TS ratio effectively, prevents from increasing with the age and ratio declines, ox bone marrow
Peptide has raising immunity function.Cytologic experiment shows that ox bone marrow Toplink effectively prevents radical pair human body
The infringement of cell membrane, the aging course of reducing human cell, can activity anti-oxidant, enhancement of SOD effectively.
Bone growth, prevention children rachitis, senile osteoporosis can be promoted, shorten the convalescence of fracture patient.
It should be appreciated that it is only exemplary that above general description and details hereinafter describe, can not
Limit the present invention.
Accompanying drawing explanation
Accompanying drawing herein is merged in specification and constitutes the part of this specification, it is shown that meet the present invention
Embodiment, and in specification together for explaining the principle of the present invention.
Fig. 1 is the method flow schematic diagram of the present invention；
Fig. 2 is the method flow schematic diagram of the embodiment of the present invention.
Detailed description of the invention
Here will illustrate exemplary embodiment in detail, its example represents in the accompanying drawings.Following retouches
Stating when relating to accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawings represents same or analogous key element.
Embodiment described in following exemplary embodiment does not represent all embodiment party consistent with the present invention
Formula.On the contrary, they only with describe in detail in appended claims, the present invention some in terms of mutually one
The example of the apparatus and method caused.
Fig. 1 is the method according to the invention schematic flow sheet, relates to a kind of ox bone marrow peptide extracting method, including
Following steps:
Cleaning destruction step 101: be carried out according to setting cleaning duration by ox bone, the ox bone after cleaning is pressed
Crush according to setting broken size.Set cleaning duration scope as 20～40 minutes, set broken size model
Enclose is 20～40 centimetres.
Boiling step 102: water is put into steaming plant, puts into the meter described ox bone of weight, and synchronizes while intensification
It is stirred, stops heating up after being warming up to set heat-preserving range, and be incubated according to setting insulation duration,
Insulation stops stirring after terminating, and stands then according to set standing duration, forms a soup.Set and protect
Temperature scope be 80～100 DEG C, sets insulation duration scope as 8～12 hours, set stand duration scope as
0.5～1.5 hour.
Row's soup step 103: utilize draw-out device to extract a described soup, and pump into a soup storage device.Take out
Fetching puts specially vacuum extraction tank.
Hydrolysing step 104 a: soup in a described soup storage device is pumped in hydrolysis device, is warming up to
Set sterilising temp and keep according to setting sterilizing duration, being then cooled to set hydrolysis temperature, add
Soup pH value regulation to neutral, put into animal protein enzyme preparation and is hydrolyzed, and keep to setting by configuration liquid
Determining enzymolysis duration, enzymolysis is warming up to set enzyme-removal temperature after terminating, and keeps to setting the enzyme duration that goes out, and is formed
Go out the hydrolyzate after enzyme.Set sterilising temp scope as 90～110 DEG C, set sterilizing duration scope as 20～40
Minute, setting hydrolysis temperature scope as 55～75 DEG C, configuration liquid is specially NaoH, sets enzymolysis duration scope
It is 4～8 hours, sets enzyme-removal temperature scope as 80～100 DEG C, set enzyme duration scope of going out as 20～40 minutes.
Filtration step 105: stand after the hydrolyzate after the described enzyme that goes out is put into diatomite and/or activated carbon, and
Utilize filter to filter to clear liquid storage device, then utilize circulation filter according to setting described clear liquid
Determine circulating filtration duration and be circulated filtration.Filter is specially tub filter and/or two-stage plate-frame filtering
Device, circulation filter is specially plate filter, sets circulating filtration duration scope as 1～3 hour.
Concentration step 106: the clear liquid after described circulating filtration is concentrated into setting and concentrates concentration, after forming clarification
Concentrate.Set concentration concentration range as 10～20 Baumes, muddy concentrate need to be recycled sheet frame mistake
Filter carries out filtering or utilize tube centrifuge to separate, and the concentrate of described muddiness is formed the dense of clarification
Contracting liquid.
Spray drying step 107: by the concentrate after described clarification according to setting EAT and setting out phoenix temperature
Degree is spray-dried, and forms ox bone marrow peptide pulvis.Set EAT scope as 190～210 DEG C, set out
Air temperature scope is 90-100 DEG C.
Fig. 2 is the method flow schematic diagram of the embodiment that the present invention provides, and relates to a kind of ox bone marrow peptide extraction side
Method, comprises the following steps:
Clean destruction step 201: ox bone was carried out according to 30 minutes, will clean after ox bone according to
25～30 centimetres crush.
Boiling step 202: water is put into steaming plant, puts into the meter described ox bone of weight, and synchronizes while intensification
It is stirred, stops after being warming up to 85～95 DEG C heating up, and be incubated according to 10 hours, after insulation terminates
Stop stirring, then according to 1 hour stands, form a soup.
Row's soup step 203: utilize vacuum extraction tank to extract a described soup, and pump into a soup storage device.
Hydrolysing step 204 a: soup in a described soup storage device is pumped in hydrolysis device, is warming up to
98～100 DEG C and kept according to 30 minutes, then it is cooled to 60～65 DEG C, adds NaoH by a soup
PH value regulation, to neutral, put into animal protein enzyme preparation and is hydrolyzed, and keep to 6 hours, and enzymolysis terminates
After be warming up to 90 DEG C, and keep to 30 minutes, form the hydrolyzate after the enzyme that goes out.
Filtration step 205: stand after the hydrolyzate after the described enzyme that goes out is put into diatomite and/or activated carbon, and
Tub filter and/or two-stage plate filter is utilized to filter to clear liquid storage device, then by described clear liquid profit
It was circulated filtration according to 2 hours with plate filter.
Concentration step 206: the clear liquid after described circulating filtration is concentrated into 13～15 Baumes, after forming clarification
Concentrate.
Spray drying step 207: by the concentrate after described clarification according to EAT 200 DEG C and leaving air temp
95 DEG C are spray-dried, and form ox bone marrow peptide pulvis.
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and utilize, to albumen
Carry out conversion and the extraction of little molecule.The ox bone marrow peptide of the present invention be that ox bone marrow is carried out biological enzymolysis extraction and
Becoming small-molecule peptide material, the essential ingredient activity in ox bone marrow peptide is superpower, is easily absorbed by the body, can be complete
Face regulation body immunity.The present invention does not contains any chemical composition and hormone, does not has the secondary work of any poison to human body
With, it is the purest nutriment.Can be dissolved in rapidly water without stirring, the aqueous solution is limpid transparent, for
White or light yellow transparent liquid, will not separate out any impurity and sediment.To the growth of human body, growth,
The physiological activity such as metabolism, procreation has vital effect.
Ox bone marrow peptide molecular weight, all below 1000 dalton, has the high property digested and assimilated and utilization rate.
It directly can be absorbed by the body without gastro-intestinal digestion.Can effectively ensure that protein is supplemented by human body.
Ox bone marrow peptide activity is strong, is easily absorbed by the body, fully adjustable body immunity.Function assessment test shows,
Ox bone marrow Toplink adjusts human body TH/TS ratio effectively, prevents from increasing with the age and ratio declines, ox bone marrow
Peptide has raising immunity function.Cytologic experiment shows that ox bone marrow Toplink effectively prevents radical pair human body
The infringement of cell membrane, the aging course of reducing human cell, can activity anti-oxidant, enhancement of SOD effectively.
Bone growth, prevention children rachitis, senile osteoporosis can be promoted, shorten the convalescence of fracture patient.
Those skilled in the art, after considering specification and putting into practice invention disclosed herein, will readily occur to this
Other bright embodiment.The application is intended to any modification, purposes or the adaptations of the present invention,
These modification, purposes or adaptations are followed the general principle of the present invention and include that the present invention is not disclosed
Common knowledge in the art or conventional techniques means.Description and embodiments is considered only as exemplary
, true scope and spirit of the invention are pointed out by claim below.It should be appreciated that the present invention
It is not limited to precision architecture described above and illustrated in the accompanying drawings, and can be without departing from its model
Enclose and carry out various modifications and changes.The scope of the present invention is only limited by appended claim.
Claims (10)
1. an ox bone marrow peptide extracting method, it is characterised in that said method comprising the steps of:
Clean destruction step, by ox bone according to set clean duration be carried out, will clean after ox bone according to
Set broken size to crush；
Boiling step, puts into steaming plant by water, puts into the meter described ox bone of weight, and same stepping while intensification
Row stirring, stops heating up after being warming up to set heat-preserving range, and is incubated according to setting insulation duration, protects
Temperature stops stirring after terminating, and stands then according to set standing duration, forms a soup；
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storage device；
Hydrolysing step, pumps in hydrolysis device by a soup in a described soup storage device, is warming up to set
Determining sterilising temp and keep according to setting sterilizing duration, being then cooled to set hydrolysis temperature, addition is joined
Put liquid by a soup pH value regulation to neutral, put into animal protein enzyme preparation and be hydrolyzed, and keep to setting
Enzymolysis duration, enzymolysis is warming up to set enzyme-removal temperature, and keeps to setting the enzyme duration that goes out after terminating, formation is gone out
Hydrolyzate after enzyme；
Filtration step, stands after the hydrolyzate after the described enzyme that goes out puts into diatomite and/or activated carbon, and utilizes
Filter filters to clear liquid storage device, then utilizes circulation filter to follow according to setting described clear liquid
Ring filters duration and is circulated filtration；
Concentration step, is concentrated into the clear liquid after described circulating filtration setting and concentrates concentration, after forming clarification
Concentrate；
Spray drying step, by the concentrate after described clarification according to setting EAT and setting leaving air temp
It is spray-dried, forms ox bone marrow peptide pulvis.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described cleaning
Setting in destruction step crushes size range as 20～40 centimetres.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described boiling
Step sets heat-preserving range as 80～100 DEG C, sets insulation duration scope as 8～12 hours.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described row's soup
Draw-out device in step is specially vacuum extraction tank.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described hydrolysis
The sterilising temp scope that sets in step, as 90～110 DEG C, sets sterilizing duration scope as 20～40 minutes.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described hydrolysis
The hydrolysis temperature scope that sets in step, as 55～75 DEG C, sets enzymolysis duration scope as 4～8 hours.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described hydrolysis
The enzyme-removal temperature scope that sets in step, as 80～100 DEG C, sets enzyme duration scope of going out as 20～40 minutes.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described filtration
Filter in step is specially tub filter and/or two-stage plate filter.
A kind of ox bone marrow peptide extracting method the most according to claim 1, it is characterised in that described concentration
Setting in step concentrates concentration range as 10～20 Baumes.
A kind of ox bone marrow peptide Advanced method the most according to claim 1, it is characterised in that described spray
The EAT scope that sets in mist drying steps, as 190～210 DEG C, sets leaving air temp scope as 90-100
℃。
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| CN201510042296.8A CN105886583A (en) | 2015-01-28 | 2015-01-28 | Bovine bone marrow peptide extraction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510042296.8A CN105886583A (en) | 2015-01-28 | 2015-01-28 | Bovine bone marrow peptide extraction method |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754151A (en) * | 2016-12-13 | 2017-05-31 | 国肽生物科技(北京)有限公司 | The preparation method of peptide wine |
| CN106854232A (en) * | 2017-03-07 | 2017-06-16 | 中国科学院新疆理化技术研究所 | A kind of preparation method and applications of bone marrow proteins in tumor |
| CN106905410A (en) * | 2017-03-07 | 2017-06-30 | 中国科学院新疆理化技术研究所 | The ultrafiltration membrane preparation method and its application of a kind of bone marrow proteins in tumor and polypeptide |
| CN107365379A (en) * | 2017-09-13 | 2017-11-21 | 甘肃中肽生物科技有限公司 | Ox bone collagen protein peptides extracting method |
| CN107853710A (en) * | 2017-11-14 | 2018-03-30 | 王清秀 | Strengthen calcium ox bone marrow peptide compound powder and its application |
| CN107874062A (en) * | 2017-11-14 | 2018-04-06 | 王清秀 | FOS ox bone marrow peptide compound powder and its application |
| CN108185407A (en) * | 2017-12-28 | 2018-06-22 | 王清秀 | The method for promoting glossy ganoderma powder absorption rate and obtained ganoderma lucidum sheep marrow peptide compound powder and application |
| CN109082453A (en) * | 2018-08-28 | 2018-12-25 | 青海七彩花生物科技有限公司 | A kind of polypeptide improves the purposes of influenza vaccines immune effect as influenza vaccines adjuvant |
| CN109182430A (en) * | 2018-08-28 | 2019-01-11 | 青海七彩花生物科技有限公司 | A kind of influenza vaccines immunologic adjuvant |
| CN110863026A (en) * | 2019-12-16 | 2020-03-06 | 宝丰县龙泉瑞鑫多肽生物制品有限公司 | Preparation method and composition of bovine or ovine spine bone marrow peptide |
| CN111235201A (en) * | 2020-03-24 | 2020-06-05 | 中科花鹿农业发展有限公司 | Deer bone peptide and extraction method thereof |
| CN113475618A (en) * | 2021-06-30 | 2021-10-08 | 内蒙古阿荣旗牧原康肽实业有限公司 | Method for preparing bovine bone collagen bone marrow peptide |
| CN113598377A (en) * | 2021-08-09 | 2021-11-05 | 河南磐康健康管理股份有限公司 | Composition for improving anemia, functional food thereof, preparation method and application |
| CN114452370A (en) * | 2021-11-24 | 2022-05-10 | 新疆农业大学 | Use of equine bone marrow enzymolysis peptide in the preparation of preparations for preventing osteoporosis |
| CN114796391A (en) * | 2022-05-10 | 2022-07-29 | 段金生 | Anti-aging health-care formula containing echinacea |
| CN115341006A (en) * | 2022-08-22 | 2022-11-15 | 育达康(北京)生物科技有限公司 | Pig bone peptide preparation, preparation method and application thereof |
| CN115624185A (en) * | 2022-10-28 | 2023-01-20 | 育达康(北京)生物科技有限公司 | Total nutrient small molecule peptide collagen preparation and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327069A (en) * | 2001-05-24 | 2001-12-19 | 谢启安 | Process for extracting amino acid polypeptide with animal bone marrow and tissue |
| CN1557466A (en) * | 2004-02-02 | 2004-12-29 | 北京世纪昭宝转移因子研究中心 | Marrow peptide preparation and its preparing process |
| CN104164466A (en) * | 2014-06-27 | 2014-11-26 | 宗树伟 | A ginseng peptide extraction method |
-
2015
- 2015-01-28 CN CN201510042296.8A patent/CN105886583A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327069A (en) * | 2001-05-24 | 2001-12-19 | 谢启安 | Process for extracting amino acid polypeptide with animal bone marrow and tissue |
| CN1557466A (en) * | 2004-02-02 | 2004-12-29 | 北京世纪昭宝转移因子研究中心 | Marrow peptide preparation and its preparing process |
| CN104164466A (en) * | 2014-06-27 | 2014-11-26 | 宗树伟 | A ginseng peptide extraction method |
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754151A (en) * | 2016-12-13 | 2017-05-31 | 国肽生物科技(北京)有限公司 | The preparation method of peptide wine |
| CN106854232A (en) * | 2017-03-07 | 2017-06-16 | 中国科学院新疆理化技术研究所 | A kind of preparation method and applications of bone marrow proteins in tumor |
| CN106905410A (en) * | 2017-03-07 | 2017-06-30 | 中国科学院新疆理化技术研究所 | The ultrafiltration membrane preparation method and its application of a kind of bone marrow proteins in tumor and polypeptide |
| CN106854232B (en) * | 2017-03-07 | 2020-10-09 | 中国科学院新疆理化技术研究所 | Preparation method and application of bone marrow protein |
| CN106905410B (en) * | 2017-03-07 | 2020-08-14 | 中国科学院新疆理化技术研究所 | Preparation method and application of bone marrow protein and polypeptide |
| CN107365379A (en) * | 2017-09-13 | 2017-11-21 | 甘肃中肽生物科技有限公司 | Ox bone collagen protein peptides extracting method |
| CN107853710A (en) * | 2017-11-14 | 2018-03-30 | 王清秀 | Strengthen calcium ox bone marrow peptide compound powder and its application |
| CN107874062A (en) * | 2017-11-14 | 2018-04-06 | 王清秀 | FOS ox bone marrow peptide compound powder and its application |
| CN108185407A (en) * | 2017-12-28 | 2018-06-22 | 王清秀 | The method for promoting glossy ganoderma powder absorption rate and obtained ganoderma lucidum sheep marrow peptide compound powder and application |
| CN109182430A (en) * | 2018-08-28 | 2019-01-11 | 青海七彩花生物科技有限公司 | A kind of influenza vaccines immunologic adjuvant |
| CN109082453A (en) * | 2018-08-28 | 2018-12-25 | 青海七彩花生物科技有限公司 | A kind of polypeptide improves the purposes of influenza vaccines immune effect as influenza vaccines adjuvant |
| CN109182430B (en) * | 2018-08-28 | 2022-10-21 | 南京赛尔健生物技术有限公司 | Influenza vaccine immunologic adjuvant |
| CN109082453B (en) * | 2018-08-28 | 2022-10-21 | 南京赛尔健生物技术有限公司 | Application of polypeptide as influenza vaccine adjuvant in improving influenza vaccine immune effect |
| CN110863026A (en) * | 2019-12-16 | 2020-03-06 | 宝丰县龙泉瑞鑫多肽生物制品有限公司 | Preparation method and composition of bovine or ovine spine bone marrow peptide |
| CN111235201A (en) * | 2020-03-24 | 2020-06-05 | 中科花鹿农业发展有限公司 | Deer bone peptide and extraction method thereof |
| CN113475618A (en) * | 2021-06-30 | 2021-10-08 | 内蒙古阿荣旗牧原康肽实业有限公司 | Method for preparing bovine bone collagen bone marrow peptide |
| CN113598377A (en) * | 2021-08-09 | 2021-11-05 | 河南磐康健康管理股份有限公司 | Composition for improving anemia, functional food thereof, preparation method and application |
| CN114452370A (en) * | 2021-11-24 | 2022-05-10 | 新疆农业大学 | Use of equine bone marrow enzymolysis peptide in the preparation of preparations for preventing osteoporosis |
| CN114452370B (en) * | 2021-11-24 | 2024-06-11 | 新疆农业大学 | Use of Ma Gusui enzymolysis peptide in preparation of preparation for preventing osteoporosis |
| CN114796391A (en) * | 2022-05-10 | 2022-07-29 | 段金生 | Anti-aging health-care formula containing echinacea |
| CN115341006A (en) * | 2022-08-22 | 2022-11-15 | 育达康(北京)生物科技有限公司 | Pig bone peptide preparation, preparation method and application thereof |
| CN115624185A (en) * | 2022-10-28 | 2023-01-20 | 育达康(北京)生物科技有限公司 | Total nutrient small molecule peptide collagen preparation and preparation method thereof |
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