CN105854107A - Method for collecting plasma of live pig - Google Patents
Method for collecting plasma of live pig Download PDFInfo
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- CN105854107A CN105854107A CN201510033368.2A CN201510033368A CN105854107A CN 105854107 A CN105854107 A CN 105854107A CN 201510033368 A CN201510033368 A CN 201510033368A CN 105854107 A CN105854107 A CN 105854107A
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- 210000002381 plasma Anatomy 0.000 claims description 97
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Abstract
The invention provides a method for collecting plasma of a live pig. The method comprises the following steps: 1, binding the whole body of the pig to be subjected to plasma collection, and fixing the bound pig to a blood collecting vehicle; 2, carrying out disinfection treatment on the selected head-neck part of the pig to be subjected to plasma collection; 3, slowly puncturing the head-neck part of the pig to be subjected to plasma collection by adopting a puncture needle connected with an injection syringe, pulling out the injection syringe when red blood appears in the injection syringe, inserting a guide wire into the puncture needle, and pulling out the puncture needle; and 4, slowly pushing a collecting catheter into the blood vessel along the guide wire, and pulling out the guide wire. With the method for collecting the plasma of the live pig, the plasma of the pig can be effectively and conveniently obtained, and does not need secondary separation, and therefore, the environmental pollution and the secondary pollution caused by the environment to the plasma are reduced; meanwhile, the initial bacterium contamination number of the plasma is reduced, the transmission of the zoonotic diseases is completely eradicated, the hemolysis phenomenon is avoided, and thus a reliable quality guarantee is provided for a biological product taking the plasma of the pig as the raw material.
Description
Technical field
The present invention relates to sampled plasma method, particularly to a kind of method for gathering live hog blood plasma.
Background technology
In the medical field, along with the fast development of medical science and technology, in order to reduce clinical medicine product to human body
The dependency of blood plasma, increasingly tends to use the blood plasma of animal.Such as, with the blood plasma of live hog for the most former
The biological medical product such as the pig source adhesive fibrin of material, it has been widely used in Clinical Surgery's hands
Art.Particularly after organ operation, this based article replaces sewing up through wound surface spraying, can quickly complete
The hemostasis of wound surface, thus decrease the diffusivity bleeding risk that patient is postoperative.So can be effectively reduced disease
The operation of people is painful, reduces clinical blood demand simultaneously, has significant benefit to society.
But, the raw material of presently relevant biological medicine enterprise is both from the collection in worksite in live hog slaughtering process.
This gatherer process is easily affected by slaughter environment, it is impossible to avoid the microorganism of related raw material and not clear impurity dirty
Dye.Sanguis sus domestica for collection in worksite needs secondary separation could obtain blood plasma, and the remaining Sanguis sus domestica after separation is difficult to
Processing, separation process not only easily causes the secondary pollution of blood plasma, and is highly vulnerable to breakage the effective active of blood plasma
Composition.
Additionally, due to whether bacteria infection or virus cannot be predicted before live hog is butchered, this method obtains
Blood plasma is difficult to solve the propagation problem of zoonosis from source, it is impossible to meet related pharmaceutical regulation to medicine
Reviewed to the requirement of product, therefore Related Drug quality is just difficult to ensure that.
Therefore, for meeting the control requirement of relevant laws and regulations, it is ensured that relevant medicine is safe and effective, it is necessary to donor
Pig closes healthy aquaculture periodically live body collection under clean environment for a long time, and those skilled in the art urgently obtain
A kind of effective and safe method gathering blood plasma.
Summary of the invention
The technical problem to be solved in the present invention is to overcome collection live hog blood plasma in prior art to be easily subject to ring
Border affects, and causes the defect of blood plasma secondary pollution, it is provided that a kind of method for gathering live hog blood plasma.
The present invention solves above-mentioned technical problem by following technical proposals: one is used for gathering live hog blood
The method of slurry, its feature is, said method comprising the steps of:
Step one, by pig whole body to be adopted binding be fixed on blood-collecting car；
Step 2, choose the head and neck position disinfection of pig to be adopted；
Step 3, with the puncture needle connecting syringe slowly puncture described in the head and neck position of pig to be adopted, to be injected
Take out syringe after seeing red blood in device, seal wire is inserted in described puncture needle, extract described puncture needle；
Step 4, collection tube is slowly pushed into Ink vessel transfusing along described seal wire, extracts described seal wire out.
It is preferred that further comprising the steps of before described step one: pig device is caught in employing will pig capture clip be adopted
Tightly, promote and overturn, and push on blood-collecting car.
It is preferred that described method also includes step 5: described collection tube is connected with described slurry consumptive material of adopting,
Described adopt slurry consumptive material and automatically adopt pulp grinder and be connected, and described in adopt and starch consumptive material and be connected with anticoagulant.
It is preferred that described automatically adopt on pulp grinder connect have blood plasma collecting bag, described blood plasma collecting bag and institute
State and adopt slurry consumptive material connection.
It is preferred that the described slurry speed of adopting automatically adopting pulp grinder is 90ml/min, feedback speed is 60ml/min,
One way collection capacity is 200ml.
It is preferred that described method also includes step 6: start described pulp grinder of automatically adopting and carry out the automatic of blood plasma
Gather.
It is preferred that described method also includes step 7: when one way gathers after blood plasma reaches 200ml, described entirely
Automatically adopt pulp grinder and automatically switch to feedback state；Automatically adopt in pulp grinder after blood cell feedback when described, then
Automatically switch to next collection one way, till the total collection capacity set completes.
It is preferred that described method also includes step 8: automatically adopt after pulp grinder quits work until described, use
The pipeline heat seal of described blood plasma collecting bag is closed by heat-sealing machine；
Step 9: retaining the length of pipe on described blood plasma collecting bag is 30cm, and remaining pipeline is wiped out, by institute
State blood plasma collecting bag be inverted, and guarantee retain described pipeline in be full of blood plasma；
Step 10: be respectively adopted heat seal at 10cm and 20cm of the described pipeline retained and close formation three sections
Detection sample cell, sticks respectively on the described detection sample cell of described blood plasma collecting bag and three sections and adopts with described waiting
Pig is identically numbered labeling；
Step 11: cut two sections of described detection sample cells, for plasma purity detection and reinspection, by described
Blood plasma collecting bag puts to the freezer of-20 DEG C preservation immediately.
It is preferred that the head and neck position choosing pig to be adopted in described step 2 is 15cm × 20cm, use purified water
Cleaning, and carry out disinfection with the ethanol of 75%.
It is preferred that a diameter of 0.021cm of described seal wire, a length of 20cm, the length of described collection tube
For 6cm.
The most progressive effect of the present invention is: the present invention can the most just for the method gathering live hog blood plasma
Obtain porcine blood plasma promptly, and described porcine blood plasma is without secondary separation, decrease environmental pollution and environment pair
The secondary pollution that blood plasma causes.The microbiological contamination number that blood plasma is initial can be reduced simultaneously, stop zoonosis
Propagation, it is to avoid haemolysis occur, improve the content of activated protein in blood plasma, thus be with porcine blood plasma
Biological product for raw material provide reliable quality assurance.
Accompanying drawing explanation
Fig. 1 is that the present invention is for gathering the collection schematic diagram of the method for live hog blood plasma.
Detailed description of the invention
Provide presently preferred embodiments of the present invention below in conjunction with the accompanying drawings, to describe technical scheme in detail.
The present invention for the method gathering live hog blood plasma mainly include live hog immunity inoculation, close healthy aquaculture,
Pig blood pathogen detection, health pig catch fixing and wait to adopt, gathers position epidermis cleaning-sterilizing processes, wears
Sting pre-buried collection tube, connect and automatically adopt the supporting consumptive material of pulp grinder, automatically collection, quick-freezing and infecting both domestic animals and human
The processes such as disease window phase detection.
Before being acquired live hog blood plasma, first require to select healthy young pig numbering to carry out foot and mouth disease, height
The animal epidemics such as pathogenic pig blue-ear disease, swine fever carry out immunity inoculation.Immune programme for children herein is according to " 2013
Year National Animal epidemic disease plan of compulsory immunization " carry out, carry out related diseases substance detection after immunity and set up immunity
Health account, failure isolates immediately.
Health pig through immunity being raised in the clean environment closed, a point circle is raised.Nursing high protein children
After pig feed to the body weight of health pig reaches 50 kilograms, change and feed into pig coarse fodder, by the body weight control of pig often
All value addeds are about 2 kilograms.
Generally, the health pig choosing body weight more than 50 kilograms carries out sampled plasma, the list of 50 kilograms-80 kilograms
Secondary slurry amount of always adopting is 1000ml；It is 1600ml that the single that body weight is 81 kilograms-100 kilograms always adopts slurry amount；Body weight
It is 2000ml that the single of more than 101 kilograms always adopts slurry amount.The interval time of every twice collection is greater than equal to 6
My god.
Fig. 1 is that the present invention is for gathering the collection schematic diagram of the method for live hog blood plasma.As it is shown in figure 1, this
The bright method for gathering live hog blood plasma comprises the following steps:
Step one: pig whole body to be adopted binding is fixed on blood-collecting car.
Adopt before pig collects blood treating, need first to use and catch pig device and pig to be adopted is caught clamping, promotes
And upset, and push on blood-collecting car, then by fixing for the binding of its whole body, thus ensure to gather blood plasma process
In pig to be adopted firm, it is to avoid it shakes off collecting device.So can provide omnidistance steady for follow-up sampled plasma
Fixed reliable rigid condition.
Step 2: choose the head and neck position disinfection of pig to be adopted.
Specifically, the region of the proper site 15cm × 20cm size that can choose pig's head cervical region position to be adopted is entered
Row preserved skin, cleans and dries preserved skin position by the purified water of 500ml, then disappear with 75% ethanol cotton balls surface
Poison processes.
Step 3: with the puncture needle connecting syringe slowly puncture described in the head and neck position of pig to be adopted, to be injected
Take out syringe after seeing red blood in device, seal wire is inserted in described puncture needle, extract described puncture needle.
Described seal wire is preferably rustless steel seal wire.The diameter of described seal wire is preferably 0.021cm, a length of
20cm。
Step 4: along described seal wire, collection tube is slowly pushed into Ink vessel transfusing, extracts described seal wire out.To gather
It is standby that Ink vessel transfusing stayed by conduit 500, and the length of described collection tube is preferably 6cm, and its diameter is preferably 20G,
I.e. approximate 0.812mm.
Step 5: collection tube 500 be connected with adopting slurry consumptive material 400, adopts slurry consumptive material 400 and automatically adopts
Pulp grinder 200 connects, and adopt slurry consumptive material 400 be connected (as shown in Figure 1) with anticoagulant 300.
When gathering blood plasma, collection tube 500, as described in above-mentioned step 4, is slowly pushed into pig 100 to be adopted
Ink vessel transfusing, thus realize the connection of pig 100 to be adopted and a whole set of acquisition system.Automatically adopting on pulp grinder 200
Connecting and have blood plasma collecting bag 600, blood plasma collecting bag 600 connects with adopting slurry consumptive material 400.Especially blood plasma is received
Collection bag 600 is hung on weighs on hook, completes the schematic diagram after assembly connection as shown in Figure 1.
Step 6: start described pulp grinder of automatically adopting and carry out the automatic collection of blood plasma.
Preferably, before being initiated, the slurry speed of adopting automatically adopting pulp grinder 200 is adjusted to 90ml/min,
Feedback speed is 60ml/min, and one way collection capacity is 200ml.Certainly these ginsengs of pulp grinder 200 are automatically adopted
Number can set total collection capacity according to the body weight of pig 100 to be adopted.When proceeding by collection blood plasma, start complete
Automatically the topping up button of pulp grinder 200 is adopted, it is ensured that be full of anticoagulant 300 in gathering pipeline, press collection button
Start to gather.
Step 7: after one way collection blood plasma reaches 200ml, described pulp grinder of automatically adopting automatically switches to back
Defeated state；Automatically adopt in pulp grinder after blood cell feedback when described, then it is single to automatically switch to next collection
Journey, till the total collection capacity set completes.
Step 8: automatically adopt after pulp grinder quits work until described, use heat-sealing machine by blood plasma collecting bag 600
Pipeline heat seal close.
Step 9: retaining the length of pipe on described blood plasma collecting bag is 30cm, and remaining pipeline is wiped out, by institute
State blood plasma collecting bag be inverted, and guarantee retain described pipeline in be full of blood plasma.
Step 10: be respectively adopted heat seal at 10cm and 20cm of the described pipeline retained and close formation three sections
Detection sample cell, sticks respectively on the described detection sample cell of blood plasma collecting bag 600 and three sections and adopts with described waiting
Pig is identically numbered labeling.
Step 11: cut two sections of described detection sample cells, for plasma purity detection and reinspection, by described
Blood plasma collecting bag puts to the freezer of-20 DEG C preservation immediately.
For same pig 100 to be adopted, after the window phase of 60 days of interval, again gather blood plasma, only before
Rear both sides gather blood plasma the most qualified after, just may determine that the blood plasma of previous (before i.e. 60 days) is
Qualified blood plasma.Specifically mode of operation is: after qualified to the detection of plasma purity, the blood plasma of corresponding numbering
To continue Cryopreservation.After general 60 days, pig of this corresponding numbering can again be acquired blood plasma or
After the detection of ear vein blood sample is qualified, the blood plasma of previous collection is qualified blood plasma, may be used for producing.
Such as rear time, sample is defective, and the most previous blood plasma is considered as defective work, and this pig numbered accordingly needs to carry out immediately
Isolation.
Owing to setting the window phase of 60 days, thus can reject completely in window phase that may be present because of
The defective blood plasma that pathogen titre is too low and cannot detect, it is thereby advantageously ensured that produce for medical product
The risk propagated without zoonosis of raw blood plasma.
In sum, the present invention for gather the method for live hog blood plasma by live hog immunity inoculation, closely
Healthy aquaculture and pig blood pathogen detection, and health pig is carried out firmly solid during gathering blood plasma
Determine, be cleaned disinfecting, puncturing a series of mode of pre-buried collection tube etc. to collection position epidermis,
Can fully automatically gather live hog blood plasma, have effectively achieved automatically adopting of quick totally-enclosed healthy live hog blood plasma
Collection.So can meet the control requirement of relevant laws and regulations, it is ensured that the safe and effective of medicine of being correlated with.
The porcine blood plasma that the method using the present invention to be used for gathering live hog blood plasma obtains, without secondary separation, decreases
The secondary pollution that blood plasma is caused by environmental pollution and environment, can reduce the microbiological contamination number that blood plasma is initial, stop
The propagation of zoonosis, it is to avoid haemolysis occurs, and improves the content of activated protein in blood plasma,
Thus provide reliable quality assurance for the biological product with porcine blood plasma as raw material.
Although the foregoing describing the detailed description of the invention of the present invention, it will be appreciated by those of skill in the art that
These are merely illustrative of, and protection scope of the present invention is defined by the appended claims.This area
These embodiments, on the premise of without departing substantially from the principle of the present invention and essence, can be made many by technical staff
Plant change or amendment, but these changes and amendment each fall within protection scope of the present invention.
Claims (10)
1. the method being used for gathering live hog blood plasma, it is characterised in that said method comprising the steps of:
Step one, by pig whole body to be adopted binding be fixed on blood-collecting car；
Step 2, choose the head and neck position disinfection of pig to be adopted；
Step 3, with the puncture needle connecting syringe slowly puncture described in the head and neck position of pig to be adopted, to be injected
Take out syringe after seeing red blood in device, seal wire is inserted in described puncture needle, extract described puncture needle；
Step 4, collection tube is slowly pushed into Ink vessel transfusing along described seal wire, extracts described seal wire out.
2. the method for gathering live hog blood plasma as claimed in claim 1, it is characterised in that in described step
Rapid one is the most further comprising the steps of: employing is caught pig device and pig to be adopted caught clamping, promotes and overturn, and pushes away
Deliver on blood-collecting car.
3. the method for gathering live hog blood plasma as claimed in claim 1, it is characterised in that described method
Also include step 5: described collection tube is connected with described slurry consumptive material of adopting, described in adopt slurry consumptive material with automatically
Adopt pulp grinder connect, and described in adopt slurry consumptive material be connected with anticoagulant.
4. the method for gathering live hog blood plasma as claimed in claim 3, it is characterised in that described entirely
Automatically adopting to connect on pulp grinder and have blood plasma collecting bag, described blood plasma collecting bag connects with described slurry consumptive material of adopting.
5. the method for gathering live hog blood plasma as claimed in claim 3, it is characterised in that described complete from
The dynamic slurry speed of adopting adopting pulp grinder is 90ml/min, and feedback speed is 60ml/min, and one way collection capacity is 200ml.
6. the method for gathering live hog blood plasma as claimed in claim 4, it is characterised in that described method
Also include step 6: start described pulp grinder of automatically adopting and carry out the automatic collection of blood plasma.
7. the method for gathering live hog blood plasma as claimed in claim 6, it is characterised in that described method
Also include step 7: after one way collection blood plasma reaches 200ml, described pulp grinder of automatically adopting automatically switches to
Feedback state；Automatically adopt in pulp grinder after blood cell feedback when described, then automatically switch to next collection
One way, till the total collection capacity set completes.
8. the method for gathering live hog blood plasma as claimed in claim 7, it is characterised in that described method
Also include step 8: automatically adopt after pulp grinder quits work until described, use heat-sealing machine by described plasma collection
The pipeline heat seal of bag is closed；
Step 9: retaining the length of pipe on described blood plasma collecting bag is 30cm, and remaining pipeline is wiped out, by institute
State blood plasma collecting bag be inverted, and guarantee retain described pipeline in be full of blood plasma；
Step 10: be respectively adopted heat seal at 10cm and 20cm of the described pipeline retained and close formation three sections
Detection sample cell, sticks respectively on the described detection sample cell of described blood plasma collecting bag and three sections and adopts with described waiting
Pig is identically numbered labeling；
Step 11: cut two sections of described detection sample cells, for plasma purity detection and reinspection, by described
Blood plasma collecting bag puts to the freezer of-20 DEG C preservation immediately.
9. the method for gathering live hog blood plasma as claimed in claim 1, it is characterised in that described step
The head and neck position choosing pig to be adopted in two is 15cm × 20cm, uses purified water cleaning, and with the ethanol of 75%
Carry out disinfection.
10. the method for gathering live hog blood plasma as claimed in claim 4, it is characterised in that described in lead
A diameter of 0.021cm, a length of 20cm, a length of 6cm of described collection tube of silk.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510033368.2A CN105854107A (en) | 2015-01-22 | 2015-01-22 | Method for collecting plasma of live pig |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510033368.2A CN105854107A (en) | 2015-01-22 | 2015-01-22 | Method for collecting plasma of live pig |
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| Publication Number | Publication Date |
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| CN105854107A true CN105854107A (en) | 2016-08-17 |
Family
ID=56623995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510033368.2A Pending CN105854107A (en) | 2015-01-22 | 2015-01-22 | Method for collecting plasma of live pig |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107396848A (en) * | 2017-07-28 | 2017-11-28 | 广州大正新材料科技有限公司 | A kind of method that bloodletting is carried out to pig |
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| CN107396848B (en) * | 2017-07-28 | 2021-06-25 | 广州大正新材料科技有限公司 | Method for sampling blood of pig |
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