CN105845959A - Microbial fuel cell for sewage water degradation - Google Patents
Microbial fuel cell for sewage water degradation Download PDFInfo
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- CN105845959A CN105845959A CN201610299193.4A CN201610299193A CN105845959A CN 105845959 A CN105845959 A CN 105845959A CN 201610299193 A CN201610299193 A CN 201610299193A CN 105845959 A CN105845959 A CN 105845959A
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- fuel cell
- microbiological fuel
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- glucose
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- 239000000446 fuel Substances 0.000 title claims abstract description 50
- 230000000813 microbial effect Effects 0.000 title abstract description 15
- 229910001868 water Inorganic materials 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title abstract description 5
- 239000010865 sewage Substances 0.000 title abstract 4
- 230000015556 catabolic process Effects 0.000 title description 4
- 238000006731 degradation reaction Methods 0.000 title description 4
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims abstract description 44
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 33
- 239000008103 glucose Substances 0.000 claims abstract description 33
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229960002477 riboflavin Drugs 0.000 claims abstract description 23
- 235000019192 riboflavin Nutrition 0.000 claims abstract description 21
- 239000002151 riboflavin Substances 0.000 claims abstract description 21
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims abstract description 14
- 241001351439 Oneida Species 0.000 claims abstract description 10
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims abstract description 7
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract description 7
- 230000000593 degrading effect Effects 0.000 claims abstract description 5
- 230000002906 microbiologic effect Effects 0.000 claims description 38
- 239000000243 solution Substances 0.000 claims description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 20
- 239000012531 culture fluid Substances 0.000 claims description 18
- 239000000126 substance Substances 0.000 claims description 14
- 239000002351 wastewater Substances 0.000 claims description 14
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 11
- 229910052799 carbon Inorganic materials 0.000 claims description 11
- 239000004744 fabric Substances 0.000 claims description 10
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 10
- 239000011707 mineral Substances 0.000 claims description 10
- 229940088594 vitamin Drugs 0.000 claims description 10
- 229930003231 vitamin Natural products 0.000 claims description 10
- 235000013343 vitamin Nutrition 0.000 claims description 10
- 239000011782 vitamin Substances 0.000 claims description 10
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 10
- -1 potassium ferricyanide phosphate Chemical group 0.000 claims description 8
- 241000863430 Shewanella Species 0.000 claims description 7
- 239000008366 buffered solution Substances 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 5
- 241000878021 Shewanella baltica Species 0.000 abstract 3
- 230000005611 electricity Effects 0.000 description 12
- 230000010287 polarization Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 7
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 6
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 4
- 229910000397 disodium phosphate Inorganic materials 0.000 description 4
- 229960003512 nicotinic acid Drugs 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- AGBQKNBQESQNJD-SSDOTTSWSA-N (R)-lipoic acid Chemical compound OC(=O)CCCC[C@@H]1CCSS1 AGBQKNBQESQNJD-SSDOTTSWSA-N 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 229930003270 Vitamin B Natural products 0.000 description 2
- 229930003451 Vitamin B1 Natural products 0.000 description 2
- 229930003471 Vitamin B2 Natural products 0.000 description 2
- 229930003537 Vitamin B3 Natural products 0.000 description 2
- AGBQKNBQESQNJD-UHFFFAOYSA-N alpha-Lipoic acid Natural products OC(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 229910052927 chalcanthite Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 2
- 235000019136 lipoic acid Nutrition 0.000 description 2
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 2
- 229910052603 melanterite Inorganic materials 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 2
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 2
- GRLPQNLYRHEGIJ-UHFFFAOYSA-J potassium aluminium sulfate Chemical compound [Al+3].[K+].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O GRLPQNLYRHEGIJ-UHFFFAOYSA-J 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 229960002663 thioctic acid Drugs 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 235000019156 vitamin B Nutrition 0.000 description 2
- 239000011720 vitamin B Substances 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- 235000019164 vitamin B2 Nutrition 0.000 description 2
- 239000011716 vitamin B2 Substances 0.000 description 2
- 235000019160 vitamin B3 Nutrition 0.000 description 2
- 239000011708 vitamin B3 Substances 0.000 description 2
- 229940084600 vitamin b 12 0.1 mg Drugs 0.000 description 2
- 229940023356 vitamin b6 10 mg Drugs 0.000 description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 239000011686 zinc sulphate Substances 0.000 description 2
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920000049 Carbon (fiber) Polymers 0.000 description 1
- 229920005479 Lucite® Polymers 0.000 description 1
- 229920000557 Nafion® Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000004917 carbon fiber Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M8/00—Fuel cells; Manufacture thereof
- H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
- H01M4/00—Electrodes
- H01M4/86—Inert electrodes with catalytic activity, e.g. for fuel cells
- H01M4/96—Carbon-based electrodes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Electrochemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Manufacturing & Machinery (AREA)
- Sustainable Development (AREA)
- Sustainable Energy (AREA)
- Inert Electrodes (AREA)
- Fuel Cell (AREA)
Abstract
The invention relates to a microbial fuel cell, and discloses a microbial fuel cell for degrading glucose sewage water to generate electric energy. The microbial fuel cell comprises a positive electrode chamber and a negative electrode changer, wherein the positive electrode chamber is loaded with sewage water and a culture solution as a positive electrode liquid, the sewage water contains xylose and/or glucose, the culture solution contains riboflavin, and the positive electrode chamber is inoculated with Oneida Shewanella baltica MR-1. The microbial fuel cell disclosed by the invention is inoculated with the Oneida Shewanella baltica MR-1, and the Oneida Shewanella baltica MR-1 can grow by means of the glucose in the microbial fuel cell to generate power; and the riboflavin is additionally added into the positive electrode liquid and is taken as an electron medium, the voltage and power density is substantially improved, the internal resistance is reduced, the glucose in the solution can be efficiently removed, the electric producing quantity of the microbial fuel cell is higher, the performance is more excellent, and the maximum power of the microbial fuel cell reaches 319.9mW/m<2> and is ten times of that of a microbial fuel cell without the riboflavin.
Description
Technical field
The present invention relates to microbiological fuel cell, be specifically related to a kind of glucose wastewater of degrading and produce the microbiological fuel cell of electric energy.
Background technology
Along with the progress and development of the mankind, all the more strong to the demand of the energy, in order to solve energy problem, the mankind seek novel energy mode positive.Microbiological fuel cell (Microbial Fuel Cells, MFCs) it is the new device utilizing microbial catalyzed degradation Organic substance to produce electric energy, refuse can be realized process and utilization of energy simultaneously, its ultimate principle is: under anode chamber's anaerobic environment, Organic substance decomposes and discharges electronics and proton under microbial action, electronics relies on suitable electron transfer mediator effectively to transmit between biological components and anode, and form electric current by the from outside negative electrode that is delivered to, and proton is delivered to negative electrode by PEM, oxidant obtains electronics at negative electrode and is reduced and is combined into water with proton.Therefore the most biodegradable material all can select suitable microorganism as the fuel of microbiological fuel cell, and the most available biomass of microbiological fuel cell produce the organic matter such as waste water, refuse produced by electricity, particularly human lives and commercial production.It is gentle that microbiological fuel cell has reaction condition, it is not necessary to carries out the advantages such as exhaust-gas treatment.
Glucose is to be distributed organic compound that is the widest and that have important biomolecule function in nature, it is widely used in the industries such as food, medicine, chemical industry and fermentable, and being discharged in environment along with waste water, the COD of many industrial wastewaters is mainly caused by glucose, becomes important environment organic contamination source.Therefore, the reproducible utilization of these organic wastewaters becomes a significant challenge of environmental improvement.
There are some researches show, certain micro-organisms can utilize glucose to produce electric energy in MFC, but these MFC electricity generation performances are poor, produces electricity relatively low, affects its application prospect, it is therefore desirable to develop a kind of more efficient microbiological fuel cell.
Summary of the invention
It is an object of the invention to provide and a kind of produce that electricity is higher, the microbiological fuel cell of excellent performance.
For reaching above-mentioned purpose, the present invention by the following technical solutions:
A kind of microbiological fuel cell of degrading waste water, including anode chamber and cathode chamber, described anode chamber is equipped with waste water and culture fluid as anolyte, and described waste water contains xylose and/or glucose sugar, described culture fluid contains riboflavin, described anode chamber inoculation Oneida Shewanella MR-1.
Further, the concentration of described riboflavin is 0.1 ~ 0.3mmol/L, and this concentration is riboflavin concentration in culture fluid.
Further, described culture fluid includes M9 saline solution, Wolfe's trace quantity mineral substance element solution, Wolfe's vitamin solution.
Further, described M9 saline solution, Wolfe's trace quantity mineral substance element solution, the volume ratio of Wolfe's vitamin solution are 10 ~ 20:1:1.
Further, described xylose and/or glucose sugar total concentration in anolyte are 1 ~ 20g/L.
Further, described cathode chamber is equipped with catholyte, and described catholyte is potassium ferricyanide phosphate buffered solution.
Further, described microbiological fuel cell also includes anode electrode, cathode electrode, PEM and external circuits.
Further, described anode electrode is 1 × 2cm2Carbon cloth, described cathode electrode is 2 × 3cm2Carbon cloth.
The formula of M9 saline solution is: 0.5g/L NaCl, 3g/L KH2PO4、17.8g/L Na2HPO4·12H2O、1 g/L NH4Cl。
The formula of Wolfe's trace quantity mineral substance element solution is: 1.5g/L complexon I, 3.0g/L MgSO4·7H2O、0.5g/L MnSO4·H2O、1.0g/L NaCl、0.1g/L FeSO4·7H2O、0.1g/L CoCl2·6H2O、0.1g/L ZnSO4·7H2O、0.1g/L CaCl2、0.01g/L CuSO4·5H2O, 0.01g/L AlK(SO4)2·12H2O、0.01g/L H3BO2、0.01g/L Na2MnO4·2H2O。
The formula of Wolfe's vitamin solution is: biotin 2.0 mg/L, vitamin B 2.0 mg/L, vitamin B6 10 mg/L, vitamin B1 10 mg/L, vitamin B2 5.0 mg/L, nicotinic acid 5.0 mg/L, vitamin B3 5.0 mg/L, vitamin B12 0.1 mg/L, para-amino benzoic acid 5.0 mg/L, thioctic acid 5.0 mg/L.
Sugared to xylose and/or glucose, riboflavin are diluted to desired concn with M9 saline solution by anolyte；The OD600 of anolyte about 2.5.
The formula of potassium ferricyanide phosphate buffered solution is: the 16.47g/L potassium ferricyanide, 3.73g/L KCl, 17.8g/L Na2HPO4·12H2O、3g/L KH2PO4。
The present invention has a following beneficial effect:
The microbe fuel cell inoculation Oneida Shewanella MR-1 of the present invention, the Glucose-grown in microbiological fuel cell can be utilized and produce electricity, additional riboflavin in anolyte, with riboflavin as electron medium, increasing substantially the glucose in voltage and power density, reduction internal resistance, and the more efficient removal solution of energy, the product electricity making microbiological fuel cell is higher, performance is more excellent, and the peak power of microbiological fuel cell reaches 319.9mW/m2, it is be not added with riboflavin 10 times.
The glucose degradation of anodic dissolution is acetic acid, even carbon dioxide by microbiological fuel cell, and exports electric energy simultaneously, reaches waste water and processes and resource reutilization effect.It is higher that the MFC of the present invention produces electricity, and the process for various glucose wastewater has good application prospect.
Accompanying drawing explanation
Fig. 1 is the structural representation of the microbiological fuel cell of embodiment 1；
Fig. 2 is m-voltage curve when being embodiment 1 microbiological fuel cell；
Fig. 3 is power density diagram and the polarization curve of embodiment 1 experimental group microbiological fuel cell；
Fig. 4 is power density diagram and the polarization curve of embodiment 1 matched group microbiological fuel cell；
Fig. 5 is the glucose consumption rate figure of embodiment 1 microbiological fuel cell.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further:
Oneida Shewanella MR-1 used by following example is purchased from ATCC American Type Culture preservation center, bacterium numbering ATCC700550.
Embodiment
1
Experimental group:
As it is shown in figure 1, the microbiological fuel cell of the present embodiment includes anode chamber 4, cathode chamber 6, anode electrode 3, cathode electrode 5, PEM 7 and external circuits 2.
Anode chamber 4 and cathode chamber 6 are polypropylene lucite, separate with Nafion 117 PEM 7.
MFC electrode pretreatment, first carbon cloth is put into immersion 8h in acetone soln, to remove the colloid of carbon fiber surface, it is then placed in the HCl solution of 2mol/L soaking 12h, remove biomass and the metal ion etc. of carbon cloth surfaces residual, clean carbon cloth with ultra-pure water, until pH value of solution is 7, finally the carbon cloth cleaned up is put into oven drying, standby after cooling.
Inserting in anode chamber 4 and cathode chamber 6 by anode electrode 3 and cathode electrode 5 respectively, anode electrode 3 is 1 × 2cm2Through pretreatment carbon cloth, described cathode electrode 5 is 2 × 3cm2Through pretreatment carbon cloth, by external circuits 2 jointed anode electrode 3 and cathode electrode 5, electricity situation is produced in external resistance 1 reaction accessing 2K in external circuits 2, accesses data collecting card 8 and produces electricity data to collect.
Add the waste water containing glucose in anode chamber 4, with M9 saline solution by the concentration dilution of glucose to 5g/L, glucose is as carbon source and electron donor.
Culture fluid is added, consisting of of culture fluid: add M9 saline solution to 30mL, 30 μ L 100 × Wolfe's trace quantity mineral substance element solution, 30 μ L 100 × Wolfe's vitamin solutions in anode chamber 4.
The formula of M9 saline solution is: 0.5g/L NaCl, 3g/L KH2PO4、17.8g/L Na2HPO4·12H2O、1g/L NH4Cl.The formula of Wolfe's trace quantity mineral substance element solution is: 1.5g/L complexon I, 3.0g/L MgSO4·7H2O、0.5g/L MnSO4·H2O、1.0g/L NaCl、0.1g/L FeSO4·7H2O、0.1g/L CoCl2·6H2O、0.1g/L ZnSO4·7H2O、0.1g/L CaCl2、0.01g/L CuSO4·5H2O, 0.01g/L AlK(SO4)2·12H2O、0.01g/L H3BO2、0.01g/L Na2MnO4·2H2O.The formula of Wolfe's vitamin solution is: biotin 2.0 mg/L, vitamin B 2.0 mg/L, vitamin B6 10 mg/L, vitamin B1 10 mg/L, vitamin B2 5.0 mg/L, nicotinic acid 5.0 mg/L, vitamin B3 5.0 mg/L, vitamin B12 0.1 mg/L, para-amino benzoic acid 5.0 mg/L, thioctic acid 5.0 mg/L.
Adding riboflavin in culture fluid, riboflavin concentration in culture fluid is 0.18mmol/L.
Inoculating Oneida Shewanella MR-1 in anode chamber 4 as electro-chemical activity microorganism, Oneida Shewanella MR-1 is with LB fluid medium activation culture, uses that 5000r/min is centrifugal goes supernatant to obtain.
Cathode chamber 6 adds the 30mL potassium ferricyanide phosphate buffered solution containing 50mmol/L, and the formula of potassium ferricyanide phosphate buffered solution is: the 16.47g/L potassium ferricyanide, 3.73g/L KCl, 17.8g/L Na2HPO4·12H2O、3g/L KH2PO4。
Matched group: the nutrient solution of accompanying of microbiological fuel cell is added without riboflavin, and other conditions are identical with experimental group.
Blank group: microbiological fuel cell does not inoculate Oneida Shewanella MR-1, and other conditions are identical with matched group.
Start and run microbiological fuel cell, anode chamber is starting and is needing in running to keep oxygen-free environment, cathode chamber is then exposed in air, the running voltage at MFC two ends is monitored in real time by data collecting card, m-voltage curve during drafting, result is as shown in Figure 2, blank group (curve 3) is almost without voltage, the voltage peak of experimental group (curve 1) is higher than the voltage peak of matched group (curve 2), the maximum voltage that experimental group produces is 578mV, the maximum voltage that matched group produces is 121mV, this shows that adding riboflavin can be greatly improved voltage, improve electricity generation performance.
Single cycle is used to survey polarization curve and the power curve of MFC.Before measuring polarization data, MFC is in open-circuit condition a few hours until voltage tends towards stability, external variable rheostat is utilized to regulate circuit dispatch from foreign news agency resistance, resistance is made the most quantitatively to successively decrease (being decremented to 330 by 20K, 10K, 9K), each resistance time of staying is 20 ~ 30 minutes, obtain the magnitude of voltage under different external resistance, and according to formula P=V2/ Rt and PAn=V2/ A R calculates the electric current density under each resistance and power density (representing electric current density and the power density of battery with the electric current of projected area A of unit anode or power), draws out power curve and polarization curve；Wherein: P is output, PAnBeing output power density, V is voltage, and R is external resistance.
Fig. 3 is power density diagram and the polarization curve of experimental group, and curve 4 represents that voltage, curve 5 represent power density, glucose MFC power density along with the increase of electric current density present first rise after downward trend, when electric current density is 735.9mA/m2, power density reaches maximum, and maximum power density is 319.9mW/m2, now corresponding external resistance is 3K, and the total internal resistance understanding MFC according to power curve method is 3K, obtains electric charge transfer internal resistance 726 by what polarization curve Slope Method (R=V/I, wherein I is electric current) calculated.
Fig. 4 is power density diagram and the polarization curve of matched group, and curve 6 represents that voltage, curve 7 represent power density, glucose MFC power density along with the increase of electric current density present first rise after downward trend, when electric current density is 125mA/m2, power density reaches maximum, and maximum power density is 32.0mW/m2, corresponding external resistance is 10K, and the total internal resistance understanding MFC according to power curve method is about 10K, by polarization curve Slope Method, obtaining current density 75 ~ 125mA/m2Polarization curve, calculating electric charge transfer internal resistance be 5316.
The above results shows, adds riboflavin and internal resistance can be greatly lowered, improve power density, and the product electricity making microbiological fuel cell is higher, and performance is more excellent.
Choose the consumption rate of the first product electricity period measurement glucose, from MFC anode chamber, take 500 μ L culture fluid every a few hours, use high performance liquid chromatograph to measure the situation of change of wherein concentration of glucose to reflect glucose degradation situation.High performance liquid chromatograph uses Shimadzu LC-20AD, and chromatographic column uses Aminex HPX-87H sugar post (300 × 7.8mm), the sulfuric acid solution of the 4mM that flowing configures mutually for ultra-pure water, detector uses Composition distribution RID 10A, flow velocity 0.4mL/min, column temperature 50 DEG C, 1 hour analysis time.Anode culture solution sample feeding device needs to carry out pretreatment: use 10,000 to leave heart 5min, takes supernatant and with the filter membrane filtration sterilization of 0.45 μm.
Result is not as it is shown in figure 5, after running 18h, the glucose of blank group (curve 8) consumes, and in matched group (curve 9) Anodic liquid, concentration of glucose reduces by 48.3%；And the concentration of glucose of experimental group (curve 10) increases along with the operation time and reduces, the glucose consumption in anolyte 99.8%.This microbiological fuel cell showing to add riboflavin can glucose in more efficient removal solution.
Embodiment
2
The microbiological fuel cell of the present embodiment, the consisting of of culture fluid in anode chamber: 30mL M9 saline solution, 15 μ L 100 × Wolfe's trace quantity mineral substance element solution, 15 μ L 100 × Wolfe's vitamin solutions；In culture fluid, the concentration of riboflavin is 0.1mmol/L；In anolyte, the concentration of glucose is 1g/L.
The structure of microbial dye battery, strain, catholyte etc. are identical with the experimental group of embodiment 1.
Embodiment
3
The microbiological fuel cell of the present embodiment, the consisting of of culture fluid in anode chamber: 30mL M9 saline solution, 20 μ L 100 × Wolfe's trace quantity mineral substance element solution, 20 μ L 100 × Wolfe's vitamin solutions；In culture fluid, the concentration of riboflavin is 0.3mmol/L；In anolyte, the concentration of glucose is 10g/L.
The structure of microbial dye battery, strain, catholyte etc. are identical with the experimental group of embodiment 1.
Embodiment
4
The microbiological fuel cell of the present embodiment, the consisting of of culture fluid in anode chamber: 30mL M9 saline solution, 25 μ L 100 × Wolfe's trace quantity mineral substance element solution, 25 μ L 100 × Wolfe's vitamin solutions；In culture fluid, the concentration of riboflavin is 0.25mmol/L；In anolyte, the total concentration of xylose and glucose is 20g/L.
The structure of microbial dye battery, strain, catholyte etc. are identical with the experimental group of embodiment 1.
The above; being only the detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, any those skilled in the art of belonging to are in the technical scope that the invention discloses; the change that can readily occur in or replacement, all should contain within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with scope of the claims.
Claims (8)
1. a microbiological fuel cell for degrading waste water, including anode chamber and cathode chamber, it is characterised in that, described anode chamber equipped with waste water and culture fluid as anolyte, described waste water contains xylose and/or glucose sugar, and described culture fluid contains riboflavin, described anode chamber inoculation Oneida Shewanella MR-1.
Microbiological fuel cell the most according to claim 1, it is characterised in that the concentration of described riboflavin is 0.1 ~ 0.3mmol/L.
Microbiological fuel cell the most according to claim 1, it is characterised in that described culture fluid includes M9 saline solution, Wolfe's trace quantity mineral substance element solution, Wolfe's vitamin solution.
Microbiological fuel cell the most according to claim 3, it is characterised in that described M9 saline solution, Wolfe's trace quantity mineral substance element solution, the volume ratio of Wolfe's vitamin solution are 10 ~ 20:1:1.
Microbiological fuel cell the most according to claim 1, it is characterised in that described xylose and/or glucose sugar total concentration in anolyte are 1 ~ 20g/L.
Microbiological fuel cell the most according to claim 1, it is characterised in that described cathode chamber is equipped with catholyte, and described catholyte is potassium ferricyanide phosphate buffered solution.
Microbiological fuel cell the most according to claim 1, it is characterised in that described microbiological fuel cell also includes anode electrode, cathode electrode, PEM and external circuits.
Microbiological fuel cell the most according to claim 7, it is characterised in that described anode electrode is 1 × 2cm2Carbon cloth, described cathode electrode is 2 × 3cm2Carbon cloth.
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| CN117568247A (en) * | 2024-01-19 | 2024-02-20 | 河南师范大学 | Preparation method and application of engineered E. coli electrocatalyst coated with metal-polyphenol nanocomplexes |
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