CN105838709A - Method of extracting DNA and RNA at same time - Google Patents
Method of extracting DNA and RNA at same time Download PDFInfo
- Publication number
- CN105838709A CN105838709A CN201610369194.1A CN201610369194A CN105838709A CN 105838709 A CN105838709 A CN 105838709A CN 201610369194 A CN201610369194 A CN 201610369194A CN 105838709 A CN105838709 A CN 105838709A
- Authority
- CN
- China
- Prior art keywords
- test tube
- dna
- rna
- liquid
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 39
- 238000007400 DNA extraction Methods 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 claims description 12
- 230000009514 concussion Effects 0.000 claims description 11
- 235000019441 ethanol Nutrition 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 239000011536 extraction buffer Substances 0.000 claims description 8
- 239000002244 precipitate Substances 0.000 claims description 7
- MFESCIUQSIBMSM-UHFFFAOYSA-N I-BCP Chemical compound ClCCCBr MFESCIUQSIBMSM-UHFFFAOYSA-N 0.000 claims description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 claims description 6
- 239000007844 bleaching agent Substances 0.000 claims description 6
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 claims description 6
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 6
- 239000012498 ultrapure water Substances 0.000 claims description 6
- 229920002527 Glycogen Polymers 0.000 claims description 5
- 229940096919 glycogen Drugs 0.000 claims description 5
- 125000005909 ethyl alcohol group Chemical group 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000008236 heating water Substances 0.000 claims description 3
- 239000006166 lysate Substances 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- CQHGAGSNWPSHHU-UHFFFAOYSA-N [O-]C#N.[NH4+].[S] Chemical compound [O-]C#N.[NH4+].[S] CQHGAGSNWPSHHU-UHFFFAOYSA-N 0.000 claims 1
- 230000001988 toxicity Effects 0.000 abstract description 3
- 231100000419 toxicity Toxicity 0.000 abstract description 3
- 238000002123 RNA extraction Methods 0.000 abstract 2
- 230000009089 cytolysis Effects 0.000 abstract 2
- 230000010355 oscillation Effects 0.000 abstract 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 239000000284 extract Substances 0.000 description 8
- 150000007523 nucleic acids Chemical class 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 108020004707 nucleic acids Proteins 0.000 description 6
- SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- SODQFLRLAOALCF-UHFFFAOYSA-N 1lambda3-bromacyclohexa-1,3,5-triene Chemical compound Br1=CC=CC=C1 SODQFLRLAOALCF-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a method of extracting DNA and RNA at the same time. The method includes: 1), disposing tissue in an oscillation tube, and adding 500 ul of tissue lysis liquid into the oscillation tube for lysis of the tissue to obtain tissue liquid; 2), transferring the tissue liquid into a centrifugal tube, adding 100 ul of 1-bromine-3-chloropropane and 20 ng/ul of glucogen in concentration into the centrifugal tube, oscillating and standing the centrifugal tube at room temperature, and centrifuging to divide liquid in the centrifugal tube into an upper layer, a middle layer and a lower layer; 3), taking a test tube A and a test tube B, pouring the liquid on the upper layer in the centrifugal tube into the test tube A for RNA extraction, and pouring the liquid on the middle and lower layers in the centrifugal tube into the test tube B for DNA extraction at the same time. The method is lower in toxicity and quicker and more efficient in DNA and RNA extraction. DNA and RNA extracted by the method are quite high in purity, and DNA and RNA can be extracted at the same time, so that working efficiency is improved greatly.
Description
Technical field
The present invention relates to a kind of method simultaneously extracting DNA and RNA.
Background technology
Clinical tissue sample is precious inhereditary material source, and the amount being obtained in that under normal circumstances is little, as
What more substantially efficiently utilizes limited clinical tissue is a problem needing solution badly.Extract at present DNA and
The method of RNA has two kinds, and one is Trizol i.e. chloroform extraction method, and another kind is Silicon moulds absorption method.Before
The chloroform that person is used is the hazardous organic solvents of a kind of easy system poison, although but the latter's acquisition simple to operate
Nucleic acid purity is on the low side, and particularly in the nucleic acid extraction of trace sample, efficiency is the lowest.
Summary of the invention
It is an object of the invention to provide a kind of method simultaneously extracting DNA and RNA.
In order to achieve the above object, the present invention is achieved by the following technical solutions:
A kind of method simultaneously extracting DNA and RNA, step is as follows:
1), by tissue being placed in concussion pipe, then adding volume in concussion pipe is 500 μ l Tissue lysates,
Tissue is cracked, obtains tissue fluid；
2), tissue fluid is transferred in centrifuge tube, in centrifuge tube, then add the 1-that volume is 100 μ l
Bromo-3-chloropropane and the glycogen that concentration is 20ng/ μ l, then carry out centrifuge tube shaking, room temperature stands, so
After be centrifuged after, centrifugal liquid in pipe is divided into three layers, and described three layers is upper strata, middle level, lower floor；
3), take test tube A and test tube B, the supernatant liquid in the centrifuge tube in step 2 is poured in test tube A
Carry out the extraction of RNA, the middle lower floor liquid in centrifuge tube is poured into simultaneously and test tube B carries out carrying of DNA
Take.
The extraction step carrying out RNA in test tube A is as follows:
The first step: the isopropanol that volume is 150 μ l is added in test tube A, test tube A is carried out successively on
Lower reverse mixing, room temperature stand, are centrifuged；
Second step: then adding 500 μ l volumetric concentrations in test tube A is the ethanol of 75%, rinses；
3rd step: after Li Xin, pours out the liquid in test tube A, then by test tube A room temperature dry to
White precipitate bleach；
4th step: continuing to add volume in test tube A is that 5ul to 50 μ l processed through pyrocarbonic acid diethyl ester
Sterilizing ultra-pure water, the volumetric concentration of described pyrocarbonic acid diethyl ester is 1 ‰, then test tube A is carried out water-bath and adds
Heat, then shakes, is centrifuged, be subsequently placed on ice, obtain RNA liquid, measure RNA purity and concentration
After RNA liquid is preserved at a temperature of subzero 80 degree.
The extraction step carrying out DNA in test tube B is as follows:
A, in test tube B, add 200 μ l DNA Extraction buffers, test tube B is carried out 55 degree of water-baths and adds
Heat 10 minutes, carries out turning upside down 5 times by test tube B during heating water bath；
B, by test tube B after room temperature carries out standing 10 minutes, then 4 degree 12000 revs/min centrifugal 15 points
Clock, now the liquid in test tube B is divided into upper and lower two-layer；
C, take test tube C, during just the supernatant liquid of test tube B is poured into test tube C, then add in test tube C
Entering volume is 250 μ l absolute ethyl alcohols；
D, test tube C is placed on temperature be under minus 20 degrees stand 1 hour, precipitate DNA, then by test tube
C carries out 4 degree 12000 revs/min and is centrifuged；
E, in test tube C add volumetric concentration be 75% ethanol, rinse, after rinsing twice, by test tube
The supernatant liquid of C is poured out, and is then dried to white precipitate bleach in room temperature by test tube C；
F, in test tube C, add 10-50ul sterilizing ultra-pure water, centrifugal after concussion, obtain DNA liquid, survey
After determining DNA purity and concentration, DNA liquid is preserved at a temperature of subzero 80 degree.
The DNA Extraction buffer of the present invention include 1M trishydroxymethylaminomethane, 3.75M different guanidine hydracid guanidine,
0.3M ammonium thiocyanate, 35mM sodium citrate.
Beneficial effects of the present invention is as follows: the present invention uses 1-bromo-3-chloropropane to replace chloroform, greatly reduces
Murder by poisoning during nucleic acid extraction, therefore the toxicity of the present invention is lower, extracts DNA and RNA safer；
In addition the present invention with the addition of appropriate glycogen while adding 1-bromo-3-chloropropane and makes the easier precipitation of nucleic acid,
Extract DNA and RNA more quick, efficiently；The present invention utilizes DNA Extraction buffer to make DNA more hold
Easily extracting, the purity of DNA and RNA that the present invention extracts is the highest, and can extract DNA simultaneously
And RNA, substantially increase operating efficiency.
Detailed description of the invention
It is described further below in conjunction with to technical scheme:
A kind of method simultaneously extracting DNA and RNA, step is as follows:
1), by tissue being placed in concussion pipe, then adding volume in concussion pipe is 500 μ l Tissue lysates,
Carrying out concussion makes tissue crack, and the time of concussion is 1 minute, obtains tissue fluid；
2), tissue fluid is transferred in centrifuge tube, in centrifuge tube, then add the 1-that volume is 100 μ l
Bromo-3-chloropropane and the glycogen that concentration is 20ng/ μ l, then carry out centrifuge tube shaking, room temperature stands 12 points
Clock, then 4 degree 12000 revs/min after centrifugal 15 minutes, centrifugal liquid in pipe is divided into three layers, and described three layers are
Upper strata, middle level, lower floor；
3), take test tube A and test tube B, the supernatant liquid in the centrifuge tube in step 2 is poured in test tube A
Carry out the extraction of RNA, the middle lower floor liquid in centrifuge tube is poured into simultaneously and test tube B carries out carrying of DNA
Take；
The extraction step carrying out RNA in test tube A is as follows:
The first step: the isopropanol that volume is 150 μ l is added in test tube A, test tube A is carried out successively on
Lower reverse mixing, room temperature stand 8 minutes, subsequently 4 degree 12000 revs/min centrifugal 15 minutes；
Second step: then adding 500 μ l volumetric concentrations in test tube A is the ethanol of 75%, rinses；
3rd step: after 4 degree 12000 revs/min centrifugal, the liquid in test tube A is poured out, then by test tube
A dries to white precipitate bleach in room temperature；
4th step: continuing to add volume in test tube A is that 5ul to 50 μ l processed through pyrocarbonic acid diethyl ester
Sterilizing ultra-pure water, the volumetric concentration of described pyrocarbonic acid diethyl ester is 1 ‰, and then test tube A carries out 59 degree of water
Bath heating 10 minutes, then shake, centrifugal after, be subsequently placed on ice, obtain RNA liquid, measure RNA
After purity and concentration, RNA liquid is preserved at a temperature of subzero 80 degree.
The extraction step carrying out DNA in test tube B is as follows:
A, in test tube B, add 200 μ l DNA Extraction buffers, test tube B is carried out heating water bath, water
During bath heating, test tube B is turned upside down；
B, being stood in room temperature by test tube B, be then centrifuged, now the liquid in test tube B is divided into
Lower two-layer；
C, take test tube C, during just the supernatant liquid of test tube B is poured into test tube C, then add in test tube C
Entering volume is 250 μ l absolute ethyl alcohols；
D, test tube C is placed on temperature be under minus 20 degrees stand 1 hour, then test tube C is centrifuged；
E, in test tube C add volumetric concentration be 75% ethanol, rinse, by the supernatant liquid of test tube C
Pour out, then test tube C is dried to white precipitate bleach in room temperature；
F, in test tube C, add 10-50ul sterilizing ultra-pure water, centrifugal after concussion, obtain DNA liquid, survey
After determining DNA purity and concentration, DNA liquid is preserved at a temperature of subzero 80 degree.
The DNA Extraction buffer of the present invention include 1M trishydroxymethylaminomethane, 3.75M different guanidine hydracid guanidine,
0.3M ammonium thiocyanate, 35mM sodium citrate.
The present invention uses 1-bromo-3-chloropropane to replace chloroform, greatly reduces the murder by poisoning during nucleic acid extraction,
Therefore the toxicity of the present invention is lower, extracts DNA and RNA safer；In addition the present invention is adding 1-bromine
With the addition of appropriate glycogen while-3-chloropropane makes nucleic acid be easier to precipitation, extracts DNA and RNA more
Fast, efficiently；The present invention utilizes DNA Extraction buffer to make DNA be easier to extract, and the present invention carries
The purity of DNA and RNA taken is the highest, and can extract DNA and RNA simultaneously, substantially increases
Operating efficiency.
It should be noted that listed above is only a kind of specific embodiment of the present invention.Obviously, the present invention
It is not limited to above example, it is also possible to have many deformation.
In a word, those of ordinary skill in the art can directly derive from present disclosure or associate
All deformation, are all considered as protection scope of the present invention.
Claims (4)
1. the method simultaneously extracting DNA and RNA, it is characterised in that step is as follows:
1), by tissue being placed in concussion pipe, then adding volume in concussion pipe is 500 μ l Tissue lysates,
Tissue is cracked, obtains tissue fluid；
2), tissue fluid is transferred in centrifuge tube, in centrifuge tube, then add the 1-that volume is 100 μ l
Bromo-3-chloropropane and the glycogen that concentration is 20ng/ μ l, then carry out centrifuge tube shaking, room temperature stands, so
After be centrifuged after, centrifugal liquid in pipe is divided into three layers, and described three layers is upper strata, middle level, lower floor；
3), take test tube A and test tube B, the supernatant liquid in the centrifuge tube in step 2 is poured in test tube A
Carry out the extraction of RNA, the middle lower floor liquid in centrifuge tube is poured into simultaneously and test tube B carries out carrying of DNA
Take.
A kind of method simultaneously extracting DNA and RNA, it is characterised in that
The extraction step carrying out RNA in described test tube A is as follows:
The first step: the isopropanol that volume is 150 μ l is added in test tube A, test tube A is carried out successively on
Lower reverse mixing, room temperature stand, are centrifuged；
Second step: then adding 500 μ l volumetric concentrations in test tube A is the ethanol of 75%, rinses；
3rd step: after Li Xin, pours out the liquid in test tube A, then by test tube A room temperature dry to
White precipitate bleach；
4th step: continuing to add volume in test tube A is that 5ul to 50 μ l processed through pyrocarbonic acid diethyl ester
Sterilizing ultra-pure water, the volumetric concentration of described pyrocarbonic acid diethyl ester is 1 ‰, then test tube A is carried out water-bath and adds
Heat, then shakes, is centrifuged, be subsequently placed on ice, obtain RNA liquid, measure RNA purity and concentration
After RNA liquid is preserved at a temperature of subzero 80 degree.
A kind of method simultaneously extracting DNA and RNA, it is characterised in that
The extraction step carrying out DNA in described test tube B is as follows:
A, in test tube B, add 200 μ l DNA Extraction buffers, test tube B is carried out heating water bath, water
During bath heating, test tube B is turned upside down；
B, being stood in room temperature by test tube B, be then centrifuged, now the liquid in test tube B is divided into
Lower two-layer；
C, take test tube C, during just the supernatant liquid of test tube B is poured into test tube C, then add in test tube C
Entering volume is 250 μ l absolute ethyl alcohols；
D, test tube C is placed on temperature be under minus 20 degrees stand 1 hour, then test tube C is centrifuged；
E, in test tube C add volumetric concentration be 75% ethanol, rinse, by the supernatant liquid of test tube C
Pour out, then test tube C is dried to white precipitate bleach in room temperature；
F, in test tube C, add 10-50ul sterilizing ultra-pure water, centrifugal after concussion, obtain DNA liquid, survey
After determining DNA purity and concentration, DNA liquid is preserved at a temperature of subzero 80 degree.
A kind of method simultaneously extracting DNA and RNA, it is characterised in that
Described DNA Extraction buffer includes 1M trishydroxymethylaminomethane, 3.75M different guanidine hydracid guanidine, 0.3M sulphur
Ammonium cyanate, 35mM sodium citrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610369194.1A CN105838709A (en) | 2016-05-27 | 2016-05-27 | Method of extracting DNA and RNA at same time |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610369194.1A CN105838709A (en) | 2016-05-27 | 2016-05-27 | Method of extracting DNA and RNA at same time |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105838709A true CN105838709A (en) | 2016-08-10 |
Family
ID=56595213
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610369194.1A Pending CN105838709A (en) | 2016-05-27 | 2016-05-27 | Method of extracting DNA and RNA at same time |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105838709A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795928A (en) * | 2018-07-02 | 2018-11-13 | 苏州呼呼健康科技有限公司 | A method of for DNA and RNA in separation and Extraction cell |
| CN112662739A (en) * | 2021-01-04 | 2021-04-16 | 深圳海普洛斯医学检验实验室 | Urine exosome RNA extraction and library construction method for NGS platform |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102952899A (en) * | 2012-11-12 | 2013-03-06 | 中国农业科学院柑桔研究所 | RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) detection method of citrus tatter leaf viruses |
| CN105368816A (en) * | 2015-11-25 | 2016-03-02 | 湖北省农业科学院畜牧兽医研究所 | Method for separating DNA, RNA and protein from single plant sample |
-
2016
- 2016-05-27 CN CN201610369194.1A patent/CN105838709A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102952899A (en) * | 2012-11-12 | 2013-03-06 | 中国农业科学院柑桔研究所 | RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) detection method of citrus tatter leaf viruses |
| CN105368816A (en) * | 2015-11-25 | 2016-03-02 | 湖北省农业科学院畜牧兽医研究所 | Method for separating DNA, RNA and protein from single plant sample |
Non-Patent Citations (1)
| Title |
|---|
| 百度文库: "血液标本的收集和保存方法", 《百度文库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108795928A (en) * | 2018-07-02 | 2018-11-13 | 苏州呼呼健康科技有限公司 | A method of for DNA and RNA in separation and Extraction cell |
| CN112662739A (en) * | 2021-01-04 | 2021-04-16 | 深圳海普洛斯医学检验实验室 | Urine exosome RNA extraction and library construction method for NGS platform |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105838709A (en) | Method of extracting DNA and RNA at same time | |
| CN104178480A (en) | Kit and method for quickly extracting DNA of plant by use of DNA adsorption column | |
| CN106769297A (en) | A kind of method that Ancient Silk Textile is determined based on proteomics | |
| CN105890945A (en) | Ultrasonic centrifuged seepage sample injecting method for quickly measuring rapidly available potassium in soil | |
| CN104164423B (en) | Kit and method for rapidly extracting plant RNA by utilizing RNA adsorption column | |
| CN106754879A (en) | A kind of plant leaf blade DNA simplifies rapid extracting method | |
| CN104031910A (en) | High-efficiency extraction method of total RNA (ribonucleic acid) in freshwater crab haemolymph | |
| CN104596826B (en) | The extracting method of albumen in a kind of stone in urinary system | |
| CN207423628U (en) | Rock core displacement device for washing oil | |
| CN105057271B (en) | Method for cleaning sample vials in batches | |
| CN106978414A (en) | A kind of nucleic acid-protein extracts reagent and its application and nucleic acid-protein extracting method | |
| CN111019940A (en) | Extracting solution for directly extracting whole blood genome DNA and extracting method thereof | |
| CN202974733U (en) | Concentrating cup assembly for pretreatment of food samples | |
| CN107523613A (en) | The method of single dinoflagellate cysts Molecular Identification | |
| CN107603975A (en) | A kind of lily method for extracting total RNA | |
| CN205352821U (en) | Portable biological sample liquid nitrogen freezes storage | |
| EA201991781A1 (en) | METHOD FOR PROCESSING TITANIUM-CONTAINING SLAG | |
| CN102433324B (en) | Circular extraction method of plant genome DNA (deoxyribonucleic acid) | |
| CN102180946B (en) | Method for concentrating and drying peptide sample | |
| CN107505308A (en) | Method for detecting trace mercury and cadmium in water through ICP extraction | |
| CN107860635B (en) | Core flooding oil washing device and method | |
| CN209410653U (en) | A kind of stable transhipment ice bag of biological reagent | |
| CN106706464A (en) | Milk fat analyzer | |
| CN104729951A (en) | Quantitative chemical analysis method of triacetate fiber and polyester fiber blended product | |
| CN105039275A (en) | Method for separating and purifying glutathion peroxidase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160810 |
|
| RJ01 | Rejection of invention patent application after publication |