CN105830720B - Cuttage breeding method for coniferous tree - Google Patents
Cuttage breeding method for coniferous tree Download PDFInfo
- Publication number
- CN105830720B CN105830720B CN201610235093.5A CN201610235093A CN105830720B CN 105830720 B CN105830720 B CN 105830720B CN 201610235093 A CN201610235093 A CN 201610235093A CN 105830720 B CN105830720 B CN 105830720B
- Authority
- CN
- China
- Prior art keywords
- cuttage
- cuttings
- soil
- matrix
- torreya
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 241001330457 Torreya jackii Species 0.000 claims abstract description 36
- 238000005520 cutting process Methods 0.000 claims abstract description 27
- 241000196324 Embryophyta Species 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 10
- 238000005507 spraying Methods 0.000 claims abstract description 7
- 238000010276 construction Methods 0.000 claims abstract description 6
- 238000012423 maintenance Methods 0.000 claims abstract description 4
- 239000002689 soil Substances 0.000 claims description 28
- 238000002360 preparation method Methods 0.000 claims description 20
- 239000011159 matrix material Substances 0.000 claims description 19
- 241000589166 Sinorhizobium fredii Species 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 241000908011 Trichoderma aureoviride Species 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 14
- 241000194108 Bacillus licheniformis Species 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 12
- 239000007921 spray Substances 0.000 claims description 10
- 210000004185 liver Anatomy 0.000 claims description 9
- 241000222122 Candida albicans Species 0.000 claims description 8
- 206010007134 Candida infections Diseases 0.000 claims description 8
- 201000003984 candidiasis Diseases 0.000 claims description 8
- 229930191978 Gibberellin Natural products 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 7
- 239000003448 gibberellin Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 238000012876 topography Methods 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000001850 reproductive effect Effects 0.000 abstract description 4
- 241000894007 species Species 0.000 abstract description 3
- 238000011160 research Methods 0.000 description 6
- 240000000147 Torreya grandis Species 0.000 description 5
- 235000016410 Torreya grandis Nutrition 0.000 description 5
- 238000010899 nucleation Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 241001135312 Sinorhizobium Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000011449 brick Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 101150068263 putA gene Proteins 0.000 description 2
- 230000002786 root growth Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 241001138444 Globularia alypum Species 0.000 description 1
- 244000082204 Phyllostachys viridis Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241000233629 Phytophthora parasitica Species 0.000 description 1
- 241000422846 Sequoiadendron giganteum Species 0.000 description 1
- 240000006909 Tilia x europaea Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 241000488908 Torreya Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000011121 hardwood Substances 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003434 inspiratory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000004016 soil organic matter Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to technical field of plant asexual propagation, specially a kind of cuttage breeding method for coniferous tree.Coniferous tree is preferably that Torreya jackii specifically includes：（1）The construction in full exposure auto spraying cuttage pond：（2）Cuttings acquires and fringe processed；（3）Cuttage；（4）Maintenance management after cuttage；（5）It is managed after transplanting.The method of the present invention is simple and practicable, and reproductive survival rate is high, and heredity variation is small, management is convenient, is conducive to large-scale breeding, can effectively save Endangered Plant Torreya jackii, and the existence background of the species is northwards elapsed.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, and in particular to the cuttage breeding method of coniferous tree.
Background technology
Coniferous tree Torreya jackii（Torreya jackii Chan）Also known as Zhejiang Chinese torreya, Jia Shi Chinese torreyas, belong to taxaceae Torreya, is
The few survivors gymnosperm of Tertiary Period remaining of new generation, the distinctive rare tree in China, the wild plant of national II grades of focused protection,
There is more important meaning to climatic similarity of Quaternary ice age etc. to the classification of research category, flora.In addition, Torreya jackii material
Matter is hard, there is fragrance, can utensil processed.
Torreya jackii is evergreen dungarunga or is often multi-branched shrub；Branch is verticillate or to life, carries out or sprig is sagging；Leaf pair
Raw, for row at two row, matter is hard, wire lanceolar shape, 3-13 centimetres long, 3-4 millimeters wide, first has gradually thorn-like tip, base portion wedge shape to have
Short handle, above light green, micro- tool 2 and unconspicuous middle arteries.Monoecism, male cone list leave armpit, tool 4-8 wheels often take turns 4 pieces
Stamen.Ellipse or oblong, base portion have decussate bract, have short stalk.Female cone is born in leaf in pairs, respectively has 2 pairs of interactions
To the squamella that raw bract and 1 piece of side are given birth to, has short stalk；Ovule Dan Sheng, uprightly, only 1 globe daisy development.Seed is all by meat
Matter aril is wrapped, oval, 2-3 centimetres long, reddish yellow when ripe, and by white powder, embryo is significantly inwardly deep to wrinkle.
The mountain valley limes marginis that the Chinese torreya that comes into leaves is grown on moistening more, feels nice and cool, under hillside private parts hardwood forest, bamboo grove and overhanging cliff
Rock gap in.It is distributed mainly on China's Southern Zhejiang, western part, In Western Fujian Province, the north, Jiangxi Province east, the north and Hubei Province north
Some areas in portion, distribution are narrower.Geographical coordinate：112 °~122 ° of east longitude, 26 °~32 ° of north latitude are distributed the northern fringe
In the townshiies Tonglu County Lu Ci of the Zhejiang Province ridges Cao Fei and Hubei Province Baokang County yellow township, one band of the big region of rivers and lakes；Nan Yuan is in Fujian Province Taining County
The townshiies Mei Kou；Hole is grown in east along in the Xianju County in Zhejiang Province townshiies An Ling stone, west to Jiangxi Province Zixi County and Jingan County state-owned forest farms.
Torreya jackii distribution is narrow, resource scarcity, the destruction with mankind's activity to natural environment, wild Torreya jackii number
Amount gradually reduces, and Torreya jackii seed maturity is long, and natural regeneration ability is poor.It is passed the winter using the storage of warm sand or is directly sowed,
Germination percentage only about 0.5-1%.Thus it is badly in need of the artificial breeding for taking measures to accelerate Torreya jackii.Plant Research Inst., Jiangsu Prov. is from 20 generation
The initial stage sixties i.e. beginning Torreya jackii of recording introduces a fine variety work, and by more than 50 years Domestication tests, Torreya jackii adapted to the gas in Nanjing
It waits, has preserved that 2 plants of trees from Zhejiang introduces a collection are 6 meters high, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is up to 13 centimetres of big tree, to by the distributed areas of Torreya jackii
From primary border northward extension.
The cutting propagation research for being directed to Torreya jackii in the prior art is considerably less, since Torreya jackii is extremely rare in the wild, city
More suitable on field not have, Torreya jackii seed maturity is very long, and natural regeneration ability is poor, is passed the winter using the storage of warm sand or is directly broadcast
Kind, germination percentage is only 0.5-1%, in addition, Torreya jackii is slow-growing, the requirement to environment is high, what the Chinese Academy of Sciences of Jiangsu Province preserved
2 plants of Torreya jackiis, more than 50 years in the past, sets 6 meters high, 13 centimetres of chest neck, but so far without solid, the situation of the prior art is unfavorable
With the large-scale breeding of Torreya jackii, therefore, Endangered Plant Torreya jackii can be saved by studying effective invisible propagation method of Torreya jackii,
And the existence background of the species is northwards elapsed.Cottage method disclosed in prior art application number 2013101994490, takes root
Rate is relatively low, cutting propagation excessive cycle, and present inventor cooperates with the Chinese Academy of Sciences, is improved on the basis of existing technology,
Obtain excellent technique effect.
Invention content
The purpose of the present invention is to provide the cuttage breeding method for coniferous tree especially Torreya jackii, this method can be kept
The good characteristic of Torreya jackii, and method is simple and practicable, reproductive survival rate is high, and preparing Torreya jackii nursery stock for energy large-scale application provides
Technical guarantee.
A kind of cuttage breeding method of Torreya jackii provided by the invention, is as follows：
1, the construction in full exposure auto spraying cuttage pond：Select flat illumination abundance, topography, well-ventilated and draining convenient
Local construction cuttage pond.Top is equipped with the automatic spraying system by time control, does not spread sunshade net, is full exposure condition.Skewer
It includes several plastic crates to insert in pond, a gauze is covered in each basket, to prevent matrix from leaking out.The lower section in the wide direction of plastic crate
The upper brick of both sides pad, to facilitate draining.
2, the preparation of cutting medium：Cuttage pond is using horse liver soil：Red soil：Turfy soil=3:3:The mixed base of 2 weight ratios
Matter, bio-organic fertilizer is added in then press mixed-matrix total weight 3%, and mixes well, laying depth 20-22cm.
3, cuttings acquisition and fringe processed：Cuttage Best Times are generally late June to late July.The acquisition of cuttings is generally selected
Rainy days or morning dew it is not dry when carry out, the acquisition current year raw sturdy branch from Torreya jackii kind tree carries out fringe processed, fringe bar make plus
Work preferably carries out indoors, or in outdoor shady and cool place.Cuttings length 10-12cm stays leaf 4-6 pieces, lower clip to be trimmed to
45 degree of bevelings, are conducive to take root；
Clip under fringe bar is immersed in complex microorganism preparations, 30min.
The complex microorganism preparations include the raw material of following weight proportion：Sinorhizobium fredii zymotic fluid：Yellowish green wood
Mould zymotic fluid：Candida fermented liquid：The lichen bacillus ferments liquid：Gibberellin：Turf：Dipotassium hydrogen phosphate=2：2：3：2：
1：5：1
The Sinorhizobium fredii is Sinorhizobium fredii（Sinorhizobium fredii）HNO1 is (referring to Fei Shi
The research of putA genes in Sinorhizobium HN01, Tang Meiqiong etc., 2009)；
The trichoderma aureoviride is trichoderma aureoviride（Trichoderma aureoviride）ACCC32248
The candidiasis is specially candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is specially for bacillus licheniformis（Bacilluslicheniformis）CGMCC
NO.2276 (referring to CN101215537A)；
The preparation method of the complex microorganism preparations is first by Sinorhizobium fredii, trichoderma aureoviride, Candida
Bacterium, bacillus licheniformis activate in a conventional manner, cultivates to viable count in bacterium solution and reaches 108A/gram acquisition zymotic fluid, will be upper
Zymotic fluid is stated according to 2：2：3：2 mass ratios mix, according to weight proportion addition gibberellin, turf and dipotassium hydrogen phosphate to obtain the final product.
4, cuttage：Cuttings cuttage depth is 3-4cm, and density seeding row spacing is the cm of 4 cm × 4；Watering after having inserted, make cuttings with
Matrix is in close contact.
5, maintenance management after cuttage：Cuttings curing condition is full exposure, and adjusts water spray frequency and spray according to weather condition
Water time length.
6, it is managed after transplanting：Transplanting medium is horse liver soil：Red soil：Turfy soil=3:3:The mixed-matrix of 2 weight ratios, and
Complex microorganism preparations are added according to 8% weight ratio in mixed-matrix, helping to take root survives.Seedling is positioned over screening early period
It under screened postive, burns to prevent sunlight burnt, after waiting 30 days or so slow seedlings, then moves to outdoor, and regard climate condition, spray is primary saturating weekly
Water.In addition, replacing big nutritive cube in time, be conducive to Torreya jackii root growth and stretching, extension.
In the present invention, in step 5, sprayed water with the automatic spraying system of time control, first 30 days after cuttage,
Time control water spray requires：It is sprayed 10 seconds within every 10 minutes in fine day；It was sprayed 10 seconds per 20-25 minutes under overcast sky；30
After it, time control water spray requirement is：It is sprayed 10 seconds within every 20 minutes in fine day；The spray 10 per 40-50 minutes under overcast sky
Second.
The beneficial effects of the present invention are：Its method is simple and practicable, and reproductive survival rate is high, and heredity changes small, manager
Just, it is conducive to large-scale breeding, can effectively saves Endangered Plant Torreya jackii, and the existence background of the species is northwards elapsed.
For plant vegetative propagation field, how plant tissue differentiation regeneration plant is utilized, improves the reproductive number of plant,
It is that study important technology emphasis be also technological difficulties to scientist, complex microorganism preparations of the invention are specifically for Torreya jackii
The nutritional preparation of asexual cuttage root-taking stimulates root cell by a large amount of investigation and experiment, the moderate concentration of gibberellin
Stretch it is vigorous, collocation Sinorhizobium fredii, trichoderma aureoviride, candidiasis, bacillus licheniformis formed one it is good micro-
The ecosystem brings the beneficial microbe colony that pest and disease damage is built with conducive to plant growth and prevented in matrix into after immersion, improve
Plant rhizosphere nutrient environment, metabolism can also reinforce the decomposition of soil organic matter, and reasonable compatibility between each strain, symbiosis is coordinated,
Mutually antagonism, microorganism secretion root system regulator and ablastins can not take good care of the rotten mould root rot of seedlings root infection, phytophthora root-rot
Sick, bacillary root rot and the sprout term diseases such as base rot disease and damping-off, samping off, dipotassium hydrogen phosphate are continuing to supply nutrition, dimension
The cuttage robust seedling impetus to be held, the adaptability of plant is greatly improved, improves entire survival rate, turf aeration is good, adsorption capacity is strong,
Retain water and nutrients are conducive to microbial activities, enhance biological property, are rich in organic matter, humic acid and other nutritional ingredients.
Horse liver soil is a kind of distinctive soil of Lower Changjiang River region, and Sha Xingqiang is dry to consolidate root, wet to be retained, water permeable ability
By force, it is most appropriate to the control of the moisture before cuttage rooting, the disadvantage is that water permeable ability is strong, dehydration is too fast, is easy to air-dry, single use
Cuttage seeding can be made to survive rear base portion dehydration withered.Red soil has good permeability and larger salt balance ability, turf
Soil property it is soft easily it is fragmentary, have it is inspiratory, be in subacidity, do not have water-retaining property.Present inventor is by a large amount of creative
Labour, carries out screening contrast experiment, screens to go into action liver soil, red soil and turfy soil these four matrix from a large amount of cutting mediums
According to horse liver soil：Red soil：Turfy soil=3:3:2 weight ratios are used in combination, and optimum Torreya jackii is taken root survival, mutual between each matrix
Make mutual induction, achievees the effect that ventilative, moisturizing, solid root.Cuttage rooting can either be rapidly promoted, and plant after cutting survival can be promoted
Growth.Using composite substrate culture Torreya jackii, Torreya jackii can be quickly and effectively obtained, solves the problems, such as its shortage.
Specific implementation mode
Embodiment 1
The construction of cuttage facility：In cuttage place research institute of Chinese Academy of Sciences nursery, selection illumination is sufficient, topography is flat, ventilation
The good and convenient local construction cuttage facility of draining.Cutting bed is the plastics Turnround basket of the cm × 18 of 55 cm × 36 cm, basket bottom
Portion is covered with a gauze, to prevent matrix from leaking out.Brick is padded on the lower section both sides in the wide direction of Turnround basket, to facilitate draining.On
The height of 80 cm of side sets up the automatic spraying system by time control；Top does not spread sunshade net, is full exposure condition.
Cutting medium：The preparation of cutting medium：Cuttage pond is using horse liver soil：Red soil：Turfy soil=3:3:2 weight ratios
Mixed-matrix, bio-organic fertilizer is added in then press mixed-matrix total weight 3%, and mixes well, laying depth 20-
22cm.It is irrigated 2 times with water before cuttage, matrix is made to suction moisture.
Cuttings acquires and fringe processed：Rainy days on July 23rd, 2014, the acquisition current year raw sturdy branch from Torreya jackii kind tree,
And branch is erected and is inserted in bucket, there is the water of deep 10 cm in bucket.Interior is taken to after 300 branches of acquisition carries out fringe processed, cuttings
Length 10-12 cm stay leaf 4-6 pieces, lower clip to be trimmed to 45 degree of bevelings, and 1 branch can be cut into 1-2 cuttings, finally there are
485 cuttings are used for cuttage, clip under fringe bar are immersed in complex microorganism preparations, 30min.
The complex microorganism preparations include the raw material of following weight proportion：Sinorhizobium fredii zymotic fluid：Yellowish green wood
Mould zymotic fluid：Candida fermented liquid：The lichen bacillus ferments liquid：Gibberellin：Turf：Dipotassium hydrogen phosphate=2：2：3：2：
1：5：1
The Sinorhizobium fredii is Sinorhizobium fredii（Sinorhizobium fredii）HNO1 is (referring to Fei Shi
The research of putA genes in Sinorhizobium HN01, Tang Meiqiong etc., 2009)；
The trichoderma aureoviride is trichoderma aureoviride（Trichoderma aureoviride）ACCC32248
The candidiasis is specially candidiasis（Candida utilis）ATCC No.22023;
The bacillus licheniformis is specially for bacillus licheniformis（Bacilluslicheniformis）CGMCC
NO.2276 (referring to CN101215537A)；
The preparation method of the complex microorganism preparations is first by Sinorhizobium fredii, trichoderma aureoviride, Candida
Bacterium, bacillus licheniformis activate in a conventional manner, cultivates to viable count in bacterium solution and reaches 108A/gram acquisition zymotic fluid, will be upper
Zymotic fluid is stated according to 2：2：3：2 mass ratios mix, according to weight proportion addition gibberellin, turf and dipotassium hydrogen phosphate to obtain the final product.
Cuttage：Cuttage depth is 3-4cm, and density seeding row spacing is 4cm × 4cm.It is watered with water sprager after having inserted, makes to insert
Fringe is in close contact with matrix.
Maintenance management after cuttage：Cuttings curing condition is full exposure, and first 30 days after cuttage, time control water spray required
For：It is sprayed 10 seconds within every 10 minutes in fine day；It was sprayed 10 seconds per 20-25 minutes under overcast sky.After 30 days, time control water spray
It is required that being：It is sprayed 10 seconds within every 20 minutes in fine day；It was sprayed 10 seconds per 40-50 minutes under overcast sky.Cuttage seeding is moved in December
Enter greenhouse.
Carry out take root investigation and transplanting in January, 2015, rooting rate 92.6% gives birth to the 449 plants of cuttage seedings taken root
Root condition survey, the every plant of root of hair number that is averaged is 4.32,6.07 cm of average root long, 0.223 cm of rough.Before the present inventor
Technology（Rooting rate 75%, mean elements 2.73, root long 4.35, rough 0.168）The time is not only saved, reduces nearly 3 months
Culture, and obtain significant effect in the parameters such as rooting rate and root long, radical.
Transplanting medium is horse liver soil：Red soil：Turfy soil=3:3:The mixed-matrix of 2 weight ratios, and pressed in mixed-matrix
Complex microorganism preparations are added according to 8% weight ratio, helping to take root survives.Seedling is positioned under sunshade net early period, to prevent the sun
The bright coke of light after waiting 30 days or so slow seedlings, then moves to outdoor, and regard climate condition, and spray is primary permeable weekly.In addition, in time more
Big nutritive cube is changed, Torreya jackii root growth and stretching, extension are conducive to.It is investigated on March 31st, 2015, acquisition altogether survives transplanting and comes into leaves
437 plants of Chinese torreya, transplanting survival rate are：97.3%.
The Torreya jackii plant obtained through the method for the present invention, growing way is strong, disease-resistant, can pass the winter safely, efficiently solve length
The vacancy of leaf Chinese torreya market resource, has broad application prospects.
Claims (2)
1. the cuttage breeding method for coniferous tree, which is characterized in that described method includes following steps：
（1）It selects flat illumination abundance, topography, well-ventilated and drains convenient local construction cuttage pond；Top be equipped with by when
Between the automatic spraying system that controls, do not spread sunshade net, be full exposure condition；
（2）The preparation of cutting medium：Cuttage pond is using horse liver soil：Red soil：Turfy soil=3:3:The mixed-matrix of 2 weight ratios, paving
If thickness is 20-22cm；
（3）Cuttings acquires and fringe processed：Cuttage Best Times are late June to late July, and rainy days or morning are selected in the acquisition of cuttings
It is carried out when dew is not dry, acquisition current year raw sturdy branch, carries out fringe processed, cuttings length 10-12cm stays leaf 4- from Torreya jackii kind tree
6, lower clip is trimmed to 45 degree of bevelings, is conducive to take root；Clip under fringe bar is immersed in complex microorganism preparations again,
30min；
（4）Cuttage：Cuttings cuttage depth is 3-4cm, and watering after having inserted makes cuttings and step（2）Gained matrix is in close contact；
（5）Maintenance management after cuttage：Cuttings curing condition is full exposure, and according to weather condition adjusting water spray frequency and when spraying water
Between length；
（6）It is managed after transplanting：Transplanting medium is horse liver soil：Red soil：Turfy soil=3:3:The mixed-matrix of 2 weight ratios, and moving
It plants in matrix and adds complex microorganism preparations according to 8% weight ratio, helping to take root survives;
The complex microorganism preparations include the raw material of following weight ratio：Sinorhizobium fredii zymotic fluid：Trichoderma aureoviride is sent out
Zymotic fluid：Candida fermented liquid：The lichen bacillus ferments liquid：Gibberellin：Turf：Dipotassium hydrogen phosphate=2：2：3：2：1：5：
1;
The Sinorhizobium fredii is Sinorhizobium fredii（Sinorhizobium fredii）HNO1；
The trichoderma aureoviride is trichoderma aureoviride（Trichoderma aureoviride）ACCC No.32248；
The candidiasis is specially candidiasis（Candida utilis）ATCC No.22023；
The bacillus licheniformis is specially bacillus licheniformis（Bacilluslicheniformis）CGMCC NO.2276.
2. according to the method described in claim 1, it is characterized in that, the preparation method of the complex microorganism preparations is：First
Sinorhizobium fredii, trichoderma aureoviride, candidiasis, bacillus licheniformis are activated in a conventional manner, cultivated to bacterium solution
Middle viable count reaches 108A/gram acquisition zymotic fluid, by zymotic fluid according to 2：2：3：2 weight ratio mix, then add gibberellin,
Turf and dipotassium hydrogen phosphate to get.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610235093.5A CN105830720B (en) | 2016-04-18 | 2016-04-18 | Cuttage breeding method for coniferous tree |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610235093.5A CN105830720B (en) | 2016-04-18 | 2016-04-18 | Cuttage breeding method for coniferous tree |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105830720A CN105830720A (en) | 2016-08-10 |
| CN105830720B true CN105830720B (en) | 2018-10-12 |
Family
ID=56588553
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610235093.5A Active CN105830720B (en) | 2016-04-18 | 2016-04-18 | Cuttage breeding method for coniferous tree |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105830720B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934505A (en) * | 2018-06-27 | 2018-12-07 | 陈东明 | A kind of method that cutting strunk urges root cuttage banyan potted landscape |
| CN108848979B (en) * | 2018-07-18 | 2021-06-22 | 陈东明 | Method for cutting and direct planting of old sycamore piles |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487719A (en) * | 2011-12-30 | 2012-06-13 | 南京金埔园林股份有限公司 | Simple cutting method for Ilex cornuta Lindli.et Paxt |
| CN102823358A (en) * | 2012-09-21 | 2012-12-19 | 南京绿宙薄壳山核桃科技有限公司 | Method for promoting lateral rooting of annual carya illinoensis seed seedling |
| CN103053377A (en) * | 2013-01-27 | 2013-04-24 | 管天球 | Vitis davidii foex culturing method |
| CN103238448A (en) * | 2013-05-27 | 2013-08-14 | 江苏省中国科学院植物研究所 | Cutting propagation method of torreya jackii chun |
| CN103555624A (en) * | 2013-10-25 | 2014-02-05 | 王天喜 | Nitrogen fixation bacillus licheniformis and application thereof |
| CN104904499A (en) * | 2014-05-09 | 2015-09-16 | 常州市金土地农牧科技服务有限公司 | Method for grafting caryaillinoinensis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101531793B1 (en) * | 2014-05-19 | 2015-06-25 | 김규석 | Method for early cuttings of Populus seedling propagation methods |
-
2016
- 2016-04-18 CN CN201610235093.5A patent/CN105830720B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487719A (en) * | 2011-12-30 | 2012-06-13 | 南京金埔园林股份有限公司 | Simple cutting method for Ilex cornuta Lindli.et Paxt |
| CN102823358A (en) * | 2012-09-21 | 2012-12-19 | 南京绿宙薄壳山核桃科技有限公司 | Method for promoting lateral rooting of annual carya illinoensis seed seedling |
| CN103053377A (en) * | 2013-01-27 | 2013-04-24 | 管天球 | Vitis davidii foex culturing method |
| CN103238448A (en) * | 2013-05-27 | 2013-08-14 | 江苏省中国科学院植物研究所 | Cutting propagation method of torreya jackii chun |
| CN103555624A (en) * | 2013-10-25 | 2014-02-05 | 王天喜 | Nitrogen fixation bacillus licheniformis and application thereof |
| CN104904499A (en) * | 2014-05-09 | 2015-09-16 | 常州市金土地农牧科技服务有限公司 | Method for grafting caryaillinoinensis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105830720A (en) | 2016-08-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105767012B (en) | A kind of accelerator beneficial to plant cuttage root-taking | |
| CN103749124B (en) | Acer palmatum overhead layering propagation method | |
| CN104145794B (en) | A kind of dendrobe tissue culture seedling heel in hardening off method | |
| CN102550252B (en) | Cultivation method of Dendrobium officinale | |
| CN102783368B (en) | White kiwi breeding and cultivating technology | |
| CN101715680A (en) | Method for promoting culture of cyclobalanopsis glauca (Thunb.) Oerst. seedlings | |
| CN103583208A (en) | Under-forest original ecology gastrodia elata planting method | |
| CN102499002B (en) | Long-distance introduction and afforestation method of acidosasa edulis | |
| CN104145795B (en) | A kind of dendrobe cultivation method improving facility utilization rate | |
| CN101707964A (en) | Tunnel half-shading green branch cottage and propagation method of large reed-leaf cherries and Teng county red cherries | |
| CN104871914A (en) | Cultivation method of red pomelo in Karst rock-desertification areas | |
| CN107046964A (en) | Begonia scale potted flower culture method | |
| CN102771297A (en) | Method for cultivating cork oak seedlings | |
| CN106171467B (en) | Rhododendron Ericatum seed propagation method | |
| CN106034936A (en) | A kind of cultivation method of Magnolia potted plant | |
| CN105993483A (en) | Organic planting method for Sechium edule | |
| CN105993605B (en) | A kind of Grifola high-yield cultivation technology | |
| CN105830720B (en) | Cuttage breeding method for coniferous tree | |
| CN106386375A (en) | Grafting cultivation method for vitis quinquangularis | |
| CN102550271A (en) | Method for commercialized cultivation of container seedling of cold-resistant japanese cinnamon germplasm | |
| CN106613659B (en) | Grafting cultivation method of euonymus plant golden beetle | |
| CN105830718B (en) | A kind of cuttage breeding method of Torreya jackii | |
| CN105028084A (en) | High-efficiency seedling raising technology of pear tree | |
| CN104871916A (en) | Method of cultivating red pomelos in conserved stony mountain lands | |
| CN105766263A (en) | Cuttage seedling propagation method for picea asperata |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190930 Address after: 221000 150 meters north of the power substation of the village of Dawu street, Jiawang District, Xuzhou, Jiangsu Patentee after: Xuzhou Jiawang District eco agricultural science and Technology Development Co., Ltd. Address before: Hangzhou City, Zhejiang province Fuyang District 311400 Fuchun Street Stadium Road No. 217 Patentee before: HANGZHOU FUYANG FEIBO TECHNOLOGY CO., LTD. |