CN105820948B - A kind of swing platform for biological respinse bag - Google Patents
A kind of swing platform for biological respinse bag Download PDFInfo
- Publication number
- CN105820948B CN105820948B CN201610197394.3A CN201610197394A CN105820948B CN 105820948 B CN105820948 B CN 105820948B CN 201610197394 A CN201610197394 A CN 201610197394A CN 105820948 B CN105820948 B CN 105820948B
- Authority
- CN
- China
- Prior art keywords
- pallet
- biological respinse
- bent axle
- respinse bag
- swing platform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 19
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000005587 bubbling Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of swing platforms for biological respinse bag, including power pedestal, pallet, bent axle and limit stick, central shaft at the top of bent axle deviates the central shaft of bottom, crankshaft installed drives rotation on power pedestal, by power pedestal, pallet is mounted on the top of bent axle by connector, rotatably, limit stick lower end is fixed with power pedestal, upper end is fixedly connected with the tray, and with pallet swing telescopic deformation occurs for limit stick.The invention enables culture solution liquid level wave features in biological respinse bag to enrich, and mixed flow is abundant, accelerates the gas-liquid mass transfer efficiency inside biological respinse bag, simultaneously effective prevents culture cell conglomeration.
Description
Technical field
The invention belongs to the technical fields of inside bioreactor gas-liquid mass transfer, and biological respinse is used for more particularly to one kind
The swing platform of bag.
Background technology
A major class in bioreactor is cell culture reactor, for cultivating zooblast and plant cell.These
Cell needs to try the gas for accelerating oxygen during growth, it is necessary to supply enough oxygen into liquid medium
Liquid mass transfer.Conventional method is using agitating paddle stir culture medium or oxygen bubble is blasted into culture medium, to increase gas
Liquid contact area, the diffusion in accelerating oxygen from the gas phase above culture medium to liquid medium.But mechanical agitation or this
Bubbling method generates higher shearing force in liquid culturing medium, causes cellular damage.For this purpose, since the last century 90's,
Wave bioreactor is progressively developed, cell and culture medium are placed in sterile disposable plastic culture bag, and
It is placed on the shake platform of special designing.The shake of platform causes culture medium to generate wave, replaces stirring mixing drum by wave
It steeps to realize the Surface Renewal between gas-liquid, both enriched with oxygen mass transfer, in turn avoided the wound of stirring slurry end of blade and bubbling to cell
Evil provides the cell culture microenvironment of mild low-shearing force dissolved oxygen relatively high simultaneously, is conducive to improve cell state, improve
Cell density and yield.
A kind of representative wave bioreactor is U.S. GE Healthcare Wave Products Group public affairs
The wave bioreactor of department shakes platform and the cell culture bags being placed on the shake platform including " seesaw type ", leads to
The seesaw type for crossing the shake platform fluctuates, and wave is generated in culture solution, but this " seesaw type " shakes platform conduct
One fluctuates entirely around intermediate support point, the wave feature and mixing shape that liquid culture is formed in reactor on platform
The angle that state can only be moved up and down by adjusting platform be adjusted, liquid culture wave feature and admixture controllability compared with
It is few, oxygen, heat transfer etc. are passed between solid culture and liquid not bery fully, wave feature and admixture adjustment may be selected in liquid
Property it is less, the swing in a direction is especially done in a square bag, lacks horizontal exchange, mixing is poor in bag.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of swing platform for biological respinse bag, biological respinse bag
Interior culture solution liquid level wave feature enriches, and mixed flow is abundant, accelerates gas-liquid mass transfer efficiency, prevents culture cell conglomeration.
The technical solution adopted by the present invention to solve the technical problems is to provide a kind of swing for biological respinse bag and puts down
Platform, including power pedestal, pallet, bent axle and limit stick, the central shaft at the top of the bent axle deviates the central shaft of bottom, the song
Axis is mounted on power pedestal, rotation is driven by power pedestal, and the pallet is mounted on bent by connector, rotatably
The top of axis, the limit stick lower end is fixed with power pedestal, upper end is fixedly connected with the tray, and limit stick is sent out with pallet swing
Raw telescopic deformation.
As a kind of preferred embodiment of the present invention, the connector is ball, on the outside of the ball with
Tray bottom is closely connected, inside and bent axle top roll cooperation installation.
As another preferred embodiment of the present invention, the connector is bearing, the bearing outside and tray bottom
Portion is closely connected, inside and bent axle top are fitted close and fix.
As another preferred embodiment of the present invention, the pallet inner wall is equipped with the flow guide bar of several protrusions.
As another preferred embodiment of the present invention, the limit stick upper end is fixed on the edge placement of pallet.
As another preferred embodiment of the present invention, the pallet is in bowl structure.
It is further improved as to the above embodiment, elliposoidal biological respinse bag is placed inside the pallet.
As another preferred embodiment of the present invention, the limit stick is made of flexible material.
As another preferred embodiment of the present invention, the power base interior be equipped with rotary speed adjustment module and
Digital display module.
Advantageous effect
In the present invention, opposite rotation can occur for the connection between pallet and bent axle, when bent axle is by power pedestal
When driving rotates, due to being acted on be subject to limit stick rotating for circumferential direction will not occur with bent axle for pallet, but and bent axle
Between opposite rotation occurs, simultaneously as bent axle upper end deviate bottom pivot, so as to drive pallet swing campaign.
Biological respinse bag is placed in inside pallet occurs swing campaign therewith, occurs to mix so as to urge the culture solution inside biological respinse bag
Stream, culture solution liquid level forms wave feature, and the mixed flow admixture that this swing urges is more abundant, culture solution liquid level
Wave feature is more abundant, the gas-liquid mass transfer efficiency inside biological respinse bag is accelerated, in addition, the culture solution of mixed flow state can
It is effectively prevented culture cell conglomeration.
Description of the drawings
Fig. 1 is the structural diagram of the present invention.
Specific embodiment
With reference to specific embodiment, the present invention is further explained.It is to be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, people in the art
Member can make various changes or modifications the present invention, and such equivalent forms equally fall within the application the appended claims and limited
Scope.
A kind of swing platform for biological respinse bag as shown in Figure 1, including power pedestal 1, pallet 2, bent axle 3 and limit
Position stick 6.
Bent axle 3 be bending shape rotation axis, at the top of central shaft deviate bottom central shaft.Bent axle 3 is mounted on dynamic
The rotary output of power pedestal 1, and rotated by the driving of power pedestal 1, power pedestal 1 is internally provided with rotary speed adjusting
Module and digital display module can be adjusted rotary speed by adjusting knob and read corresponding rotation by digital display screen
Rotary speed.
Pallet 2 is whole in bowl structure, biological respinse bag prevent with inside pallet 2, to select the biological respinse of elliposoidal
Bag is optimal.Pallet 2 is mounted on the top of bent axle 3 by connector 4, and opposite turn can occur between pallet 2 and bent axle 3
It is dynamic.Connector 4 can select to use ball, be closely connected on the outside of 2 bottom of pallet and ball, bent axle 3
The fit inside of top and ball is installed and can occur to roll relatively, passes through the ball rolling action of ball
Pallet 2 can relatively rotate between bent axle 3；Connector 4 can also use bearing, outside and 2 bottom of pallet of bearing
It is closely connected, inside and 3 top of bent axle of bearing are fitted close the self-characteristic fixed and pass through bearing so that support
Disk 2 can relatively rotate between bent axle 3.
Limit stick 6 is made of flexible material, has certain telescopic deformation characteristic.The lower end of limit stick 6 and power base
Seat 1 is fixed, and upper end is fixedly connected with pallet 2, and when bent axle 3 rotates, pallet 2 will not be under the action of limit stick 6
Rotation in circumferential direction occurs for bent axle 3, generates opposite rotation between pallet 2 and bent axle 3 at this time, simultaneously as 3 upper end of bent axle is inclined
Pivot from bottom, so as to which pallet 2 be driven to make swing campaign, with 2 swing of pallet telescopic deformation occurs for limit stick 6, but
It is that will not change on angle orientation.In order to play the effect that preferably limitation pallet 2 rotates in a circumferential direction, 6 upper end of limit stick
It is fixed on the edge placement of pallet 2.
Bent axle 3 rotates a circle, and pallet 2 therewith enclose by swing one, when continuously swing campaign occurs for pallet 2, biological respinse
Bag is placed in inside pallet 2 occurs swing campaign therewith, and mixed flow, culture solution occurs so as to urge the culture solution inside biological respinse bag
Liquid level forms wave feature, and the mixed flow admixture that this swing urges is more abundant, the wave feature of culture solution liquid level
It is more abundant, the gas-liquid mass transfer efficiency inside biological respinse bag is accelerated, prevents culture cell conglomeration.It is in addition, in order to enable raw
The culture solution of object reaction bag bottom obtains preferably mixed flow effect, is provided with the flow guide bar 5 of several protrusions in 2 inner wall of pallet, leads
Stream item 5 can change the original flow direction of biological respinse bag bottom culture solution, achieve the effect that fluid stirs.
Claims (9)
1. a kind of swing platform for biological respinse bag, including power pedestal (1), pallet (2), bent axle (3) and limit stick
(6), the central shaft at the top of the bent axle (3) deviates the central shaft of bottom, it is characterised in that：The bent axle (3) is mounted on power
Rotation is driven on pedestal (1), by power pedestal (1), the pallet (2) is mounted on bent by connector (4), rotatably
The top of axis (3), limit stick (6) lower end is fixed with power pedestal (1), upper end is fixedly connected with pallet (2), limit stick
(6) as telescopic deformation occurs for pallet (2) swing.
2. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The connector
(4) it is ball, is closely connected with pallet (2) bottom on the outside of the ball, is rolled at the top of inside and bent axle (3)
Cooperation installation.
3. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The connector
(4) it is bearing, is closely connected on the outside of the bearing with pallet (2) bottom, tight fit is solid at the top of inside and bent axle (3)
It is fixed.
4. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The pallet (2)
Inner wall is equipped with the flow guide bar (5) of several protrusions.
5. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The limit stick
(6) upper end is fixed on the edge placement of pallet (2).
6. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The pallet (2)
In bowl structure.
7. a kind of swing platform for biological respinse bag according to claim 6, it is characterised in that：The pallet (2)
Place elliposoidal biological respinse bag in inside.
8. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The limit stick
(6) it is made of flexible material.
9. a kind of swing platform for biological respinse bag according to claim 1, it is characterised in that：The power pedestal
(1) rotary speed adjustment module and digital display module are internally provided with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610197394.3A CN105820948B (en) | 2016-03-31 | 2016-03-31 | A kind of swing platform for biological respinse bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610197394.3A CN105820948B (en) | 2016-03-31 | 2016-03-31 | A kind of swing platform for biological respinse bag |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105820948A CN105820948A (en) | 2016-08-03 |
| CN105820948B true CN105820948B (en) | 2018-06-01 |
Family
ID=56525514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610197394.3A Active CN105820948B (en) | 2016-03-31 | 2016-03-31 | A kind of swing platform for biological respinse bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105820948B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418894A (en) * | 2017-08-15 | 2017-12-01 | 南京航空航天大学 | A kind of shake platform for wave bioreactor Piezoelectric Driving |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639160A (en) * | 1995-03-15 | 1997-06-17 | Kishimoto; Masaru | Platform shaker in three dimensional motion |
| CN201545840U (en) * | 2009-09-21 | 2010-08-11 | 惠识瑶 | Packed bed cell bioreactor of stirring-free device |
| CN101875026A (en) * | 2010-08-17 | 2010-11-03 | 金卫医疗科技(上海)有限公司 | Rotary limit device applied to differential centrifugal separation system |
| CN201713522U (en) * | 2010-04-30 | 2011-01-19 | 惠倪 | Swinging cell reactor with polygonal plastic bag |
| CN102482633A (en) * | 2009-07-06 | 2012-05-30 | 弗·哈夫曼-拉罗切有限公司 | Method of culturing eukaryotic cells |
| CN205576147U (en) * | 2016-03-31 | 2016-09-14 | 上海拜高乐生物技术有限公司 | A balance platform soon for biological reaction bag |
-
2016
- 2016-03-31 CN CN201610197394.3A patent/CN105820948B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5639160A (en) * | 1995-03-15 | 1997-06-17 | Kishimoto; Masaru | Platform shaker in three dimensional motion |
| CN102482633A (en) * | 2009-07-06 | 2012-05-30 | 弗·哈夫曼-拉罗切有限公司 | Method of culturing eukaryotic cells |
| CN201545840U (en) * | 2009-09-21 | 2010-08-11 | 惠识瑶 | Packed bed cell bioreactor of stirring-free device |
| CN201713522U (en) * | 2010-04-30 | 2011-01-19 | 惠倪 | Swinging cell reactor with polygonal plastic bag |
| CN101875026A (en) * | 2010-08-17 | 2010-11-03 | 金卫医疗科技(上海)有限公司 | Rotary limit device applied to differential centrifugal separation system |
| CN205576147U (en) * | 2016-03-31 | 2016-09-14 | 上海拜高乐生物技术有限公司 | A balance platform soon for biological reaction bag |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105820948A (en) | 2016-08-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5075234A (en) | Fermentor/bioreactor systems having high aeration capacity | |
| CN107349893A (en) | A kind of efficient chemical reagent mediation reactor | |
| Cheng et al. | Strengthening mass transfer of carbon dioxide microbubbles dissolver in a horizontal tubular photo-bioreactor for improving microalgae growth | |
| JPS63317074A (en) | Fermentation apparatus | |
| CN1323352A (en) | Mixer systems | |
| CN108585188A (en) | A kind of quick aerator of sewage disposal with oxygen supply function | |
| CN207169724U (en) | A kind of efficient chemical reagent mediation reactor | |
| CN105148823A (en) | Inflatable stirring reaction device | |
| CN108660056A (en) | A kind of swing type shaking table for microculture | |
| CN105820948B (en) | A kind of swing platform for biological respinse bag | |
| CN106378075B (en) | A kind of reaction kettle of floating ball positioning de-bubble | |
| CN205576147U (en) | A balance platform soon for biological reaction bag | |
| CN206304624U (en) | A kind of bio-pharmaceuticals agitating device | |
| CN208883866U (en) | A multi-material stirring hydrolysis device | |
| CN206474150U (en) | A kind of novel reaction kettle | |
| CN108641898A (en) | A kind of swinging shaking table for microculture | |
| CN201626957U (en) | biogas mixer | |
| CN204865826U (en) | Reaction unit is aerifyd in stirring | |
| CN209685769U (en) | A kind of inclined rotating drum installation for fermenting | |
| CN202786259U (en) | Bioreactor | |
| JP2011036189A (en) | Stirring device | |
| CN206396254U (en) | Asparagus liquid strain culture tank | |
| CN206736121U (en) | One kind stirring aeration type organic fertilizer fermenting apparatus | |
| CN209702605U (en) | A kind of organic fertilizer stirring fermentation system | |
| CN206940908U (en) | Antisitic defect NK cell culture apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20180510 Address after: 201611 1, 80 Lane 6965, North Song highway, Che Dun Town, Songjiang District, Shanghai. Applicant after: SHANGHAI GAOJI BIOENGINEERING CO., LTD. Address before: 201203 3 floors, No. 400, No. 400, Fang Chun Road, Shanghai Applicant before: SHANGHAI BE GAULLE BIOTECHNOLOGY CO., LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220328 Address after: 201603 floor 3, building 1, No. 400, Fangchun Road, Pudong New Area, Shanghai Patentee after: SHANGHAI BAIGAOLE BIOTECHNOLOGY CO.,LTD. Address before: 201611 1, 80 Lane 6965, North Song highway, Che Dun Town, Songjiang District, Shanghai. Patentee before: SHANGHAI GAOJI BIOENGINEERING Co.,Ltd. |