CN105816475A - Styptic powder and preparation method and application thereof - Google Patents
Styptic powder and preparation method and application thereof Download PDFInfo
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- CN105816475A CN105816475A CN201510000589.XA CN201510000589A CN105816475A CN 105816475 A CN105816475 A CN 105816475A CN 201510000589 A CN201510000589 A CN 201510000589A CN 105816475 A CN105816475 A CN 105816475A
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- 239000000843 powder Substances 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 229920002472 Starch Polymers 0.000 claims abstract description 78
- 239000008107 starch Substances 0.000 claims abstract description 78
- 235000019698 starch Nutrition 0.000 claims abstract description 78
- 238000002156 mixing Methods 0.000 claims abstract description 63
- 229920001592 potato starch Polymers 0.000 claims abstract description 34
- 238000005406 washing Methods 0.000 claims abstract description 21
- 238000004132 cross linking Methods 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 13
- 238000001035 drying Methods 0.000 claims abstract description 9
- 230000001804 emulsifying effect Effects 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 48
- 238000000034 method Methods 0.000 claims description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 36
- 238000003756 stirring Methods 0.000 claims description 34
- 238000013517 stratification Methods 0.000 claims description 32
- 239000012153 distilled water Substances 0.000 claims description 30
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- 239000000725 suspension Substances 0.000 claims description 23
- UGTZMIPZNRIWHX-UHFFFAOYSA-K sodium trimetaphosphate Chemical compound [Na+].[Na+].[Na+].[O-]P1(=O)OP([O-])(=O)OP([O-])(=O)O1 UGTZMIPZNRIWHX-UHFFFAOYSA-K 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 16
- 208000032843 Hemorrhage Diseases 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 239000006185 dispersion Substances 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 10
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- 239000008158 vegetable oil Substances 0.000 claims description 10
- 238000006266 etherification reaction Methods 0.000 claims description 9
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims description 8
- 238000005538 encapsulation Methods 0.000 claims description 8
- 235000013336 milk Nutrition 0.000 claims description 8
- 239000008267 milk Substances 0.000 claims description 8
- 210000004080 milk Anatomy 0.000 claims description 8
- 238000004806 packaging method and process Methods 0.000 claims description 8
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 238000009461 vacuum packaging Methods 0.000 claims description 8
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 claims description 5
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- 239000012295 chemical reaction liquid Substances 0.000 claims description 4
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- 239000000203 mixture Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
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- 125000002091 cationic group Chemical group 0.000 claims description 2
- 239000002270 dispersing agent Substances 0.000 claims description 2
- 239000001341 hydroxy propyl starch Substances 0.000 claims description 2
- 235000013828 hydroxypropyl starch Nutrition 0.000 claims description 2
- 206010053476 Traumatic haemorrhage Diseases 0.000 claims 1
- 238000005303 weighing Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 5
- 230000000740 bleeding effect Effects 0.000 abstract description 4
- 231100000252 nontoxic Toxicity 0.000 abstract description 4
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
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- 239000004382 Amylase Substances 0.000 description 1
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- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 1
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- 206010071229 Procedural haemorrhage Diseases 0.000 description 1
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Abstract
The invention relates to a styptic powder and its preparation method. The preparation method comprises the following steps: using potato starch and etherified starch as raw materials, processing potato starch by a super-high pressure technology, uniformly mixing the processed potato starch and etherified starch, emulsifying and dispersing, cross-linking, extracting and washing, and drying and sterilizing so as to obtain the product. The styptic powder has good biocompatibility, can directly act on common bleeding, deep bleeding and bleeding of parts where surgical operation is hard to reach, and is simple and convenient to use. By using starch as a raw material, the styptic powder is nontoxic and nonirritating, has extensive sources and is low-cost. The technology is simple and feasible, and the product has a wide clinical application prospect.
Description
Technical field
The invention belongs to biomedical materials field, be specifically related to a kind of styptic powder and preparation method thereof, application.
Background technology
First aid, wound etc. various hemorrhage be one of problem needing solution badly in surgical operation, normal human is respectively provided with hemostatic function, the most a small amount of hemorrhage self function can be relied on to stop blooding, but, the phenomenon bodies such as the disguised oozing of blood of, Viscera rupture hemorrhage for human body artery in various sudden accidents can not stop blooding voluntarily, can not only lean on common tourniquet bandage hemostasis by compression, intraoperative hemorrhage too much makes operating time extend, it is possible that serious complication, such as hemorrhagic shock, even entail dangers to life and health.The most conventional effective Hemostasis is the resorbable material hemostasis of employing biocompatibility, provides a kind of safe and effective in surgical operation, first aid etc. are stopped blooding, and easy to use, hemostatic material with low cost is particularly important.
At present, the hemostatic material used clinically mainly has Fibrin Glue, collagen protein, gelfoam and cellulose hemostatic material, these materials all achieve good effect in zoopery and clinical practice, but the most all come with some shortcomings: its raw material sources of collagen, gelatin are in animal tissue, it is foreign protein, rejection easily occurs, there is potential sensitization, and it is relatively slow to absorb degraded, can increase the infection risk of wound；Cellulose family, human body lacks makes its enzyme degraded, and degradation time is long, may bring the side effect such as infection to patient.
Potato starch is a kind of naturally occurring plant polysaccharide, its good biocompatibility, nontoxic, non-stimulated, be difficult to cause the anaphylaxis of body, monosaccharide can be degraded to by the amylase in body fluid in vivo, degradation in vivo absorbs.Etherification starch is a class modified starch, is the hydroxyl in starch molecule and the starch substituent group ether of reactivity substance reaction generation, improves viscosity stability, stable in the basic conditions, has stronger water absorption and dilatancy.The most representative starch hemostatic material is Arista styptic powder produced in USA, although its haemostatic effect is good, but it uses epichlorohydrin cross-linked, there is genotoxic potential, and expensive, limits its application.
Using superhigh pressure technique to process starch, moisture enters inside starch granules, ruptures with intermolecular hydrogen bonding in making starch molecule, crystalline texture is destroyed, and ordered structure is disturbed, and improves the reactivity of starch, use superhigh pressure technique to process starch, environmentally safe simultaneously.Styptic powder prepared by the present invention, has the strongest water absorption and water-retaining property, has efficient anastalsis, and preparation technology is simple simultaneously, it is easy to industrialized production.
Summary of the invention
In order to solve the deficiencies in the prior art, the present invention provides a kind of styptic powder and preparation method thereof, this styptic powder has good biocompatibility, body energy degraded and absorbed, catabolite safety non-toxic is non-stimulated, preparation technology simple possible, can be done directly on the most hemorrhage, Hemorrhage in Deep and the unapproachable position of operation technique is hemorrhage, easy to use.
The preparation method of a kind of styptic powder, comprises the steps:
Being processed through superhigh pressure technique by potato starch, then mix with etherification starch soluble in water by the potato starch processed through superhigh pressure technique, through emulsion dispersion, crosslinking, extracting washing, dry sterilization i.e. obtains this styptic powder.
Further, the described step that potato starch carries out superhigh pressure technique process is particularly as follows: load in high pressure resistant bag by the starch milk that mass volume ratio is 10-20%, vacuum packaging machine sealing packaging, encapsulation two-layer, the pressure chamber putting into extra-high tension unit after fully shaking up processes, high pressure cavity temperature is 20 ± 5 DEG C, boost with the speed of 10MPa/s, pressure is 600-700MPa, and the dwell time is 5-15min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Further, described by soluble in water for the prepared potato starch box etherification starch mixing through superhigh pressure technique process, through emulsion dispersion, crosslinking, extracting washing, dry sterilization i.e. obtains the step of styptic powder, specifically includes:
Produce mixing starch suspension: by weight for (0.1-100): the potato starch processed through superhigh pressure technique and etherification starch are mixed in the distilled water being dissolved in 20-40 times by (100-0.1), after stirring, regulate pH to 9-10 with sodium hydroxide solution；
Produce mixing starch emulsified dispersed liquid: weigh Span 80 and Tween 80 mix homogeneously by weight for 1:1, make blended emulsifier, add vegetable oil mixing, stir, make mixing and emulsifying dispersant, being then added in described mixing starch suspension, 45-70 DEG C of stirring 10-30min, to stirring, prepares mixing starch emulsified dispersed liquid；Vegetable oil is 15-100:1 with the envelope-bulk to weight ratio of mixing starch, ml/g, and blended emulsifier is 0.5-2.5:1, g/g with the weight ratio of mixing starch；
Cross-linking reaction: weigh a certain amount of sodium trimetaphosphate and be dissolved in distilled water, be evenly stirred until and be completely dissolved, prepares sodium trimetaphosphate solution, is then added in described mixing starch emulsified dispersed liquid, stirring, and cross-linking reaction 4-8h prepares cross-linking reaction liquid；Sodium trimetaphosphate is 0.01-0.2:1, g/g with the mass ratio of mixing starch；
Prepare styptic powder: by obtained cross-linking reaction liquid stratification, abandon supernatant, the ethyl acetate of 5-10 times of volume is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 3-5 times of volume, vacuum adds the distilled water wash of 3-5 times of volume after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Particularly, one or more during described etherification starch is carboxymethyl starch, hetastarch, hydroxypropyl starch and cationic starch etc..
Product of the present invention uses good biocompatibility, degradable absorbs, the starch of catabolite safety non-toxic is that raw material prepares, bleeding part can be directly acted on, absorbing rapidly the moisture in blood, reduce blood flowing speed, erythrocyte and PC increase, blood self-solidifying, plays the effect of hemostasis by compression after material imbibition simultaneously.
Product preparation process of the present invention is simple, with low cost, can be used for various the most hemorrhage operating, Hemorrhage in Deep and the unapproachable position of operation technique is hemorrhage, easy to use.
Detailed description of the invention
In order to be better understood from technical scheme, it is described in further detail by embodiment in detail below, but protection scope of the present invention is not limited only to following embodiment.
Embodiment 1:
Weigh 200g potato starch to be dissolved in distilled water, make the starch milk that mass volume ratio is 10%, it is loaded into again in high pressure resistant bag, vacuum packaging machine sealing packaging, double-deck encapsulation, put into high pressure equipment pressure chamber after fully shaking up to process, high pressure cavity temperature is 15 DEG C, boosts with the speed of 10MPa/s, and pressure is 600MPa, dwell time is 5min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Weigh potato starch 0.01g, the carboxymethyl starch 10g mixing processed through superhigh pressure technique to be dissolved in the distilled water of 210ml, after stirring, regulate pH to 9-10 by NaOH solution, prepare mixing starch suspension；
Weigh 3g Span 80 respectively and Tween 80 mixing is dissolved in the vegetable oil of 200ml, stir, prepare mixing and emulsifying dispersion liquid, then add it in described mixing starch suspension, 50 DEG C of stirring 10min, prepare mixing starch emulsified dispersed liquid；
Weigh 0.15g sodium trimetaphosphate to be dissolved in distilled water, be stirred well to be completely dissolved, prepare sodium trimetaphosphate solution, add it in described mixing starch emulsified dispersed liquid, stirring, cross-linking reaction 4h, prepare crosslinked fluid；
By obtained crosslinked fluid stratification, abandon supernatant, the ethyl acetate of 5 times of volumes is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 3 times of volumes, vacuum adds the distilled water wash of 3 times of volumes after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Embodiment 2:
Weigh 200g potato starch to be dissolved in distilled water, make the starch milk that mass volume ratio is 15%, it is loaded into again in high pressure resistant bag, vacuum packaging machine sealing packaging, double-deck encapsulation, put into high pressure equipment pressure chamber after fully shaking up to process, high pressure cavity temperature is 20 DEG C, boosts with the speed of 10MPa/s, and pressure is 600MPa, dwell time is 5min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Weigh potato starch 5g, the carboxymethyl starch 10g mixing processed through superhigh pressure technique to be dissolved in the distilled water of 400ml, after stirring, regulate pH to 9-10 by NaOH solution, prepare mixing starch suspension；
Weigh 7.5g Span 80 respectively and Tween 80 mixing is dissolved in the vegetable oil of 750ml, stir, prepare mixing and emulsifying dispersion liquid, then add it in described mixing starch suspension, 70 DEG C of stirring 15min, prepare mixing starch emulsified dispersed liquid；
Weigh 1.2g sodium trimetaphosphate to be dissolved in distilled water, be stirred well to be completely dissolved, prepare sodium trimetaphosphate solution, add it in described mixing starch emulsified dispersed liquid, stirring, cross-linking reaction 5h, prepare crosslinked fluid；
By obtained crosslinked fluid stratification, abandon supernatant, the ethyl acetate of 7 times of volumes is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 4 times of volumes, vacuum adds the distilled water wash of 3 times of volumes after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Embodiment 3:
Weigh 200g potato starch to be dissolved in distilled water, make the starch milk that mass volume ratio is 15%, it is loaded into again in high pressure resistant bag, vacuum packaging machine sealing packaging, double-deck encapsulation, put into high pressure equipment pressure chamber after fully shaking up to process, high pressure cavity temperature is 20 DEG C, boosts with the speed of 10MPa/s, and pressure is 650MPa, dwell time is 10min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Weigh potato starch 7g, the carboxymethyl starch 7g mixing processed through superhigh pressure technique to be dissolved in the distilled water of 560ml, after stirring, regulate pH to 9-10 by NaOH solution, prepare mixing starch suspension；
Weigh 12g Span 80 respectively and Tween 80 mixing is dissolved in the vegetable oil of 980ml, stir, prepare mixing and emulsifying dispersion liquid, then add it in described mixing starch suspension, 65 DEG C of stirring 20min, prepare mixing starch emulsified dispersed liquid；
Weigh 1.4g sodium trimetaphosphate to be dissolved in distilled water, be stirred well to be completely dissolved, prepare sodium trimetaphosphate solution, add it in described mixing starch emulsified dispersed liquid, stirring, cross-linking reaction 7h, prepare crosslinked fluid；
By obtained crosslinked fluid stratification, abandon supernatant, the ethyl acetate of 7 times of volumes is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 5 times of volumes, vacuum adds the distilled water wash of 3 times of volumes after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Embodiment 4:
Weigh 200g potato starch to be dissolved in distilled water, make the starch milk that mass volume ratio is 20%, it is loaded into again in high pressure resistant bag, vacuum packaging machine sealing packaging, double-deck encapsulation, put into high pressure equipment pressure chamber after fully shaking up to process, high pressure cavity temperature is 25 DEG C, boosts with the speed of 10MPa/s, and pressure is 650MPa, dwell time is 10min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Weigh potato starch 10g, the carboxymethyl starch 5g mixing processed through superhigh pressure technique to be dissolved in the distilled water of 600ml, after stirring, regulate pH to 9-10 by NaOH solution, prepare mixing starch suspension；
Weigh 15g Span 80 respectively and Tween 80 mixing is dissolved in the vegetable oil of 1300ml, stir, prepare mixing and emulsifying dispersion liquid, then add it in described mixing starch suspension, 45 DEG C of stirring 25min, prepare mixing starch emulsified dispersed liquid；
Weigh 1.5g sodium trimetaphosphate to be dissolved in distilled water, be stirred well to be completely dissolved, prepare sodium trimetaphosphate solution, add it in described mixing starch emulsified dispersed liquid, stirring, cross-linking reaction 8h, prepare crosslinked fluid；
By obtained crosslinked fluid stratification, abandon supernatant, the ethyl acetate of 8 times of volumes is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 5 times of volumes, vacuum adds the distilled water wash of 5 times of volumes after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Embodiment 5:
Weigh 200g potato starch to be dissolved in distilled water, make the starch milk that mass volume ratio is 20%, it is loaded into again in high pressure resistant bag, vacuum packaging machine sealing packaging, double-deck encapsulation, put into high pressure equipment pressure chamber after fully shaking up to process, high pressure cavity temperature is 25 DEG C, boosts with the speed of 10MPa/s, and pressure is 700MPa, dwell time is 15min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Weigh potato starch 10g, the carboxymethyl starch 0.01g mixing processed through superhigh pressure technique to be dissolved in the distilled water of 350ml, after stirring, regulate pH to 9-10 by NaOH solution, prepare mixing starch suspension；
Weigh 10g Span 80 respectively and Tween 80 mixing is dissolved in the vegetable oil of 900ml, stir, prepare mixing and emulsifying dispersion liquid, then add it in described mixing starch suspension, 55 DEG C of stirring 30min, prepare mixing starch emulsified dispersed liquid；
Weigh 1.5g sodium trimetaphosphate to be dissolved in distilled water, be stirred well to be completely dissolved, prepare sodium trimetaphosphate solution, add it in described mixing starch emulsified dispersed liquid, stirring, cross-linking reaction 8h, prepare crosslinked fluid；
By obtained crosslinked fluid stratification, abandon supernatant, the ethyl acetate of 10 times of volumes is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 5 times of volumes, vacuum adds the distilled water wash of 5 times of volumes after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Embodiment 6:
Weigh 200g potato starch to be dissolved in distilled water, make the starch milk that mass volume ratio is 20%, it is loaded into again in high pressure resistant bag, vacuum packaging machine sealing packaging, double-deck encapsulation, put into high pressure equipment pressure chamber after fully shaking up to process, high pressure cavity temperature is 20 DEG C, boosts with the speed of 10MPa/s, and pressure is 700MPa, dwell time is 5min, after process, stratification, abandon supernatant, in lower floor's suspension, add washing with alcohol 3-5 time, be drying to obtain the potato starch of ultra high pressure treatment.
Weigh potato starch 10g, the hetastarch 2g mixing processed through superhigh pressure technique to be dissolved in the distilled water of 420ml, after stirring, regulate pH to 9-10 by NaOH solution, prepare mixing starch suspension；
Weigh 6g Span 80 respectively and Tween 80 mixing is dissolved in the vegetable oil of 850ml, stir, prepare mixing and emulsifying dispersion liquid, then add it in described mixing starch suspension, 60 DEG C of stirring 30min, prepare mixing starch emulsified dispersed liquid；
Weigh 2g sodium trimetaphosphate to be dissolved in distilled water, be stirred well to be completely dissolved, prepare sodium trimetaphosphate solution, add it in described mixing starch emulsified dispersed liquid, stirring, cross-linking reaction 8h, prepare crosslinked fluid；
By obtained crosslinked fluid stratification, abandon supernatant, the ethyl acetate of 10 times of volumes is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 5 times of volumes, vacuum adds the distilled water wash of 5 times of volumes after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
Claims (6)
1. the preparation method of a styptic powder, it is characterised in that potato starch is first processed through superhigh pressure technique, again by soluble in water, through emulsion dispersion, cross-linking reaction for potato starch and the etherification starch mix homogeneously through ultra high pressure treatment, extracting washing, dry sterilization i.e. obtains this styptic powder.
2. the preparation method of styptic powder as claimed in claim 1, it is characterized in that, the described step processed through superhigh pressure technique by potato starch is particularly as follows: load in high pressure resistant bag by the starch milk that mass volume ratio is 10-20%, vacuum packaging machine sealing packaging, encapsulation two-layer, the pressure chamber putting into extra-high tension unit after fully shaking up processes, high pressure cavity temperature is 20 ± 5 DEG C, boost with the speed of 10MPa/s, pressure is 600-700MPa, dwell time is 5-15min, after process, stratification, abandon supernatant, washing with alcohol is added 3-5 time in lower floor's suspension, it is drying to obtain the potato starch of ultra high pressure treatment.
3. the preparation method of as claimed in claim 2 styptic powder, it is characterised in that described the potato starch processed through superhigh pressure technique and etherification starch are blended soluble in water, through emulsion dispersion, crosslinking, extracting washing, dry sterilization i.e. obtains the step of styptic powder and specifically includes:
Prepare mixing starch suspension: by weight for (0.1-100): potato starch and etherification starch that (100-0.1) weighs through superhigh pressure technique process are blended in the distilled water being dissolved in 20-40 times, regulate pH to 9-10 by NaOH solution after stirring；
Prepare mixing starch emulsified dispersed liquid: by weight weighing Span 80 and Tween 80 mix homogeneously for 1:1, prepare blended emulsifier, add vegetable oil to stir, prepare mixing and emulsifying dispersant, it is then added in mixing starch, under the conditions of 45-75 DEG C, stirring 10-30min is to stirring, and prepares mixing starch emulsified dispersed liquid；Vegetable oil is 15-100:1 with the envelope-bulk to weight ratio of mixing starch, ml/g, and blended emulsifier is 0.5-2.5:1, g/g with the weight ratio of mixing starch；
Cross-linking reaction: weigh a certain amount of sodium trimetaphosphate and be dissolved in distilled water, be evenly stirred until and be completely dissolved, prepares sodium trimetaphosphate solution, is then added in described mixing starch emulsified dispersed liquid, stirring, and cross-linking reaction 4-8h prepares cross-linking reaction liquid；Sodium trimetaphosphate is 0.01-0.2:1, g/g with the mass ratio of mixing starch；
Prepare styptic powder: by above-mentioned prepared cross-linking reaction liquid stratification, abandon supernatant, the ethyl acetate of 5-10 times of volume is added in lower floor's milky white liquid, it is sufficiently stirred for rear stratification, takes off a layer milky white liquid, add the absolute ethanol washing of 3-5 times of volume, vacuum adds the distilled water wash of 3-5 times of volume after draining, being repeated 3 times, the lower floor's milky white liquid obtained by last stratification carries out freeze-day with constant temperature, sterilizing, thus obtaining the product styptic powder.
4. the preparation method of the styptic powder as described in claim 1 or 3, it is characterised in that described etherification starch is one or more in carboxymethyl starch, hetastarch, hydroxypropyl starch and cationic starch etc..
5. the styptic powder that the method as described in Claims 1-4 prepares.
6. the hemostasis that the application of styptic powder as claimed in claim 5, it is characterised in that being applied to various traumatic hemorrhage, surgical operation wound surface is hemorrhage, oozing of blood, Hemorrhage in Deep and the unapproachable position of operation technique are hemorrhage etc..
Priority Applications (1)
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| CN112439538A (en) * | 2020-10-16 | 2021-03-05 | 中南林业科技大学 | Method for separating starch with different particle sizes |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455857A (en) * | 2007-12-11 | 2009-06-17 | 美国淀粉医疗公司 | Biocompatibility modified starch sponges |
| CN101497670A (en) * | 2008-01-29 | 2009-08-05 | 纪欣 | Biocompatibility pre-gelatinized modified starch and preparation thereof |
| CN102139123A (en) * | 2011-03-25 | 2011-08-03 | 杜宝堂 | Method for preparing intra-operative hemostatic material by cross emulsification of plant starch |
-
2015
- 2015-01-04 CN CN201510000589.XA patent/CN105816475A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455857A (en) * | 2007-12-11 | 2009-06-17 | 美国淀粉医疗公司 | Biocompatibility modified starch sponges |
| CN101497670A (en) * | 2008-01-29 | 2009-08-05 | 纪欣 | Biocompatibility pre-gelatinized modified starch and preparation thereof |
| CN102139123A (en) * | 2011-03-25 | 2011-08-03 | 杜宝堂 | Method for preparing intra-operative hemostatic material by cross emulsification of plant starch |
Non-Patent Citations (2)
| Title |
|---|
| 徐忠、缪铭编著: "《功能性变性淀粉》", 30 April 2010, 中国轻工业出版社 * |
| 李昌文等: "超高压对马铃薯淀粉糊化作用的研究", 《粮食与饲料工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112439538A (en) * | 2020-10-16 | 2021-03-05 | 中南林业科技大学 | Method for separating starch with different particle sizes |
| CN112439538B (en) * | 2020-10-16 | 2022-08-05 | 中南林业科技大学 | Method for separating starch with different particle sizes |
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