CN105806767A - Fluid circuit system of flow cytometer and flow cytometer - Google Patents

Abstract

The invention discloses a liquid path system of a cytometer and the cytometer. The liquid path system of the cytometer includes a first pump body, a second pump body, a flow chamber, a sample providing device, and a sheath fluid container. One end of the first pump body It communicates with the flow chamber, and the other end communicates with the sheath liquid container, which is used to pump the sheath liquid in the sheath liquid container into the flow chamber; the first end of the flow chamber communicates with the first pump body, and the second end communicates with the second pump body, A pressure difference is formed between the first pump body and the second pump body, and the sample providing device communicates with the flow chamber for providing sample liquid to the flow chamber according to the pressure difference. The technical solution of the present invention controls the rotational speeds of the first pump body and the second pump body through the first pump body and the second pump body, so as to stabilize the flow velocity of the liquid flow and form a stable laminar flow.

Description

Fluid system of cytometer and cytometer

technical field

The invention relates to the field of cell detection, in particular to a liquid circuit system of a cytometer and the cytometer.

Background technique

Flow cytometry is an instrument for multi-parameter, quantitative analysis or sorting of cells or biological particles in a fast-flowing state. Flow cytometry refers to the fluid control technology in which the sample cell flow is wrapped by the sheath flow, and the cells line up through the detection area of the flow chamber. The precision, stability and response speed of fluid control affect the development of flow cytometry and the application of flow cytometry. In the liquid path of the flow cytometer, the principle of hydrodynamic focusing is used to converge the sheath flow and the sample flow in the flow chamber, so that the sample flow forms a stable laminar flow with controllable size and speed, so that the optical system can detect stable cells. sample. Flow cytometry mainly detects cells, and the size, shape, material composition and other characteristics of cells have very high requirements on the accuracy, speed and stability of the liquid flow control of the detection instrument, which is also a factor that determines the analytical performance and value of flow cytometry. Key factor.

In the liquid circuit system of the previous flow cytometer, the control of the liquid circuit was often performed through a single pump body. Since the single closed-loop control controls the flow rate and flow rate of the sheath liquid and samples, it relies more on the motor control of the electric pump. The reliability is poor, and the control accuracy is poor after long-term use.

Contents of the invention

The main purpose of the present invention is to provide a fluid circuit system of the cytometer, aiming at precisely controlling the accuracy, speed and stability of the liquid flow in the fluid circuit system of the cytometer.

In order to achieve the above object, the liquid circuit system of the cytometer proposed by the present invention includes a first pump body, a second pump body, a flow chamber, a sample providing device, and a sheath fluid container,

One end of the first pump body communicates with the flow chamber, and the other end communicates with the sheath fluid container, for pumping the sheath fluid in the sheath fluid container into the flow chamber;

The first end of the flow chamber communicates with the first pump body, the second end communicates with the second pump body, a pressure difference is formed between the first pump body and the second pump body, and the A sample providing device communicates with the flow chamber for providing sample liquid to the flow chamber according to the pressure difference.

Optionally, a first pressure sensor is provided between the first pump body and the flow chamber, and a second pressure sensor is provided between the flow chamber and the second pump body.

Optionally, the first pump body and the second pump body are peristaltic pumps.

Optionally, the liquid circuit system of the cytometer further includes a waste liquid pool, and the waste liquid pool is in communication with the second pump body.

Optionally, the liquid circuit system of the cytometer further includes a cleaning liquid pool, the cleaning liquid pool is filled with cleaning liquid, the cleaning liquid pool is in communication with the first pump body, and the cleaning liquid pool is connected to the A first control valve is arranged between the first pump body.

Optionally, a second control valve is provided on the main line between the second pump body and the flow chamber, and a branch line is also provided between the second pump body and the flow chamber, and on the branch line A third control valve is provided.

Optionally, the liquid circuit system of the cytometer further includes a third pump body and a fourth pump body, the third pump body is provided with two pipelines, one of which communicates with the sheath fluid container, The other pipeline is in communication with the cleaning solution pool;

The fourth pump body communicates with the waste liquid pool through a pipeline.

Optionally, a first air pressure sensor and a first hydraulic pressure sensor are arranged in the sheath fluid container, a second air pressure sensor and a second hydraulic pressure sensor are arranged in the cleaning liquid pool, and a third air pressure sensor is arranged in the waste liquid pool and the third hydraulic pressure sensor.

Optionally, a first damper is further provided between the first pressure sensor and the first pump body, and a second damper is further provided between the second pressure sensor and the second pump body.

The present invention also provides a cytometer, including a controller and a fluid circuit system of the cytometer, and the controller is electrically connected to the first pump body and the second pump body of the fluid circuit system of the cytometer.

The technical solution of the present invention controls the rotational speeds of the first pump body and the second pump body through the first pump body and the second pump body, so as to stabilize the flow velocity of the liquid flow and form a stable laminar flow. At the same time, the rotation speeds of the first pump body and the second pump body can be adjusted to adapt to the detection of different samples. Specifically, when the sample is tested, the sheath fluid is drawn from the sheath fluid container through the first pump body, and then enters the flow chamber; the rotational speeds of the first pump body and the second pump body are different, and the pressure difference generated between the two makes the sample The sample in the supply device enters the flow chamber, mixes with the sheath liquid to form a detection liquid flow, and is detected in the flow chamber; at the same time, the second pump body pumps out the waste liquid after detection in the flow chamber.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. For those skilled in the art, other drawings can also be obtained according to the structures shown in these drawings without creative effort.

Fig. 1 is a schematic diagram of the liquid path of an embodiment of the liquid path system of the cytometer of the present invention;

Fig. 2 is a schematic flowchart of the fault diagnosis of the fluid system of the cytometer shown in Fig. 1;

Fig. 3 is a control module diagram of the cytometer of the present invention.

Explanation of reference numbers:

The realization of the purpose of the present invention, functional characteristics and advantages will be further described in conjunction with the embodiments and with reference to the accompanying drawings.

detailed description

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present invention are only used to explain the relationship between the components in a certain posture (as shown in the accompanying drawings). Relative positional relationship, movement conditions, etc., if the specific posture changes, the directional indication will also change accordingly.

In addition, in the present invention, descriptions such as "first", "second" and so on are used for description purposes only, and should not be understood as indicating or implying their relative importance or implicitly indicating the quantity of indicated technical features. Thus, the features defined as "first" and "second" may explicitly or implicitly include at least one of these features. In addition, the technical solutions of the various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist , nor within the scope of protection required by the present invention.

The invention provides a liquid path system of a cytometer.

Referring to Fig. 1 to Fig. 3, Fig. 1 is a schematic diagram of the fluid path of an embodiment of the fluid path system of the cytometer of the present invention; Fig. 2 is a schematic flow chart of the fault diagnosis of the fluid path system of the cytometer shown in Fig. 1; Fig. 3 is The control module diagram of the cytometer of the present invention.

In the embodiment of the present invention, please refer to FIG. 1 , the fluid circuit system 100 of the cytometer includes a first pump body 11 , a second pump body 12 , a flow chamber 20 , a sample providing device 30 , and a sheath fluid container 40 ,

One end of the first pump body 11 communicates with the flow chamber 20, and the other end communicates with the sheath fluid container 40, for pumping the sheath fluid in the sheath fluid container 40 into the flow chamber 20;

The first end of the flow chamber 20 communicates with the first pump body 11 , and the second end communicates with the second pump body 12 , forming a gap between the first pump body 11 and the second pump body 12 . pressure difference, the sample providing device 30 communicates with the flow chamber 20 and is used for providing sample liquid to the flow chamber 20 according to the pressure difference.

Wherein, the first pump body 11 and the second pump body 12 are peristaltic pumps. The peristaltic pump has the advantages of high repeatability and high stability precision. At the same time, it has good sealing performance, has good self-priming ability, can run dry, can prevent backflow, and has the same flow delivery capacity in both directions.

In addition, referring to FIG. 1 , the liquid circuit system 100 of the cytometer further includes a waste liquid pool 60 , which communicates with the second pump body 12 and is used to receive the waste liquid after detection. The size and shape of the waste liquid pool 60 can be set according to actual conditions.

The technical solution of the present invention controls the rotational speeds of the first pump body 11 and the second pump body 12 through the first pump body 11 and the second pump body 12 to stabilize the flow velocity of the liquid flow and form a stable laminar flow. At the same time, the rotation speeds of the first pump body 11 and the second pump body 12 can be adjusted to adapt to the detection of different samples. Specifically, during sample detection, the sheath fluid is drawn from the sheath fluid container 40 through the first pump body 11, and then enters the flow chamber 20; the rotational speeds of the first pump body 11 and the second pump body 12 are different, and the pressure difference, so that the sample in the sample providing device 30 enters the flow chamber 20, mixes with the sheath fluid to form a detection liquid flow, and is detected in the flow chamber 20; The waste liquid is transported to the waste liquid pool 60.

Referring to FIG. 1 , a first pressure sensor 51 is provided between the first pump body 11 and the flow chamber 20 , and a second pressure sensor 52 is provided between the flow chamber 20 and the second pump body 12 . Through the first pressure sensor 51 and the second pressure sensor 52, the pressure of the liquid before entering the flow chamber 20 and the pressure of the liquid after flowing out of the flow chamber 20 can be monitored in real time. The fluid pressure can reflect the fluid flow and velocity, so as to adjust The operating speed of the first pump body 11 and the second pump body 12.

At the same time, the first pressure sensor 51 and the second pressure sensor 52 feed back the fluid pressure during the sample detection process in real time. When the fluid path is blocked, by observing the abnormal pressure changes of the two sensors from the detection end, the fault diagnosis and troubleshooting of the blockage can be realized.

Referring to FIG. 2 , p1 in FIG. 2 represents the first pressure sensor 51 , and p2 represents the second pressure sensor 52 . The specific fault judgment of this hydraulic system is: if the liquid flow pressure is stable, the diagnosis is that the liquid circuit is normal.

The first kind of blockage situation: if it is first detected that the pressure of the first pressure sensor 51 decreases, then the pressure of the second pressure sensor 52 decreases, and the change between the two continues to decrease, it means that the pipeline before the first pressure sensor 51 is blocked ;

The second blockage situation: if it is first detected that the pressure of the second pressure sensor 52 decreases, and then the pressure of the first pressure sensor 51 decreases, and the changes between the two continue to decrease, but the decrease is small, it is the sample provided. The pipeline of the device 30 is blocked;

The third blockage situation: if it is first detected that the pressure of the second pressure sensor 52 becomes smaller, and then it is detected that the pressure of the first pressure sensor 51 becomes larger, then the flow chamber 20 is blocked;

The fourth clogging situation: if first the pressure of the second pressure sensor 52 is detected to be increased, and then the pressure of the first pressure sensor 51 is detected to be increased, then the pipeline behind the second pressure sensor 52 is blocked.

In this embodiment, the liquid circuit system 100 of the cytometer further includes a cleaning liquid pool 70, the cleaning liquid pool 70 contains cleaning liquid, and the cleaning liquid pool 70 communicates with the first pump body 11, A first control valve 101 is provided between the cleaning liquid pool 70 and the first pump body 11 .

Wherein, the first control valve 101 can be a three-way valve, the first end of the three-way valve communicates with the first pump body 11 , the second end communicates with the cleaning liquid pool 70 , and the third end communicates with the sheath liquid container 40 .

When the first and fourth clogging conditions are detected, the third end of the first control valve 101 is closed, and the pipeline of the sheath fluid container 40 is closed. Open the first end and the second end of the first control valve 101, and adjust the rotational speeds of the first pump body 11 and the second pump body 12 to make them consistent. In this way, the sample of the sample providing device 30 will not be sucked into the flow chamber 20, and the cleaning liquid flows through the entire liquid circuit, which can clean the blocked pipeline, so that the pipeline before the first pressure sensor 51 and the pipeline after the second pressure sensor 52 pipeline is unblocked.

When the second blockage is detected, the sample of the sample providing device 30 is replaced with a cleaning solution, and the speeds of the first pump body 11 and the second pump body 12 remain unchanged, so that the pipeline of the sample providing device 30 can be dredged .

Further, referring to FIG. 1 , a second control valve 102 is provided on the main road between the second pump body 12 and the flow chamber 20 , and a second control valve 102 is provided between the second pump body 12 and the flow chamber 20 . There is a branch pipeline, and a third control valve 103 is arranged on the branch pipeline.

When the third blockage is detected, the first end and the second end of the first control valve 101 are opened, the first pump body 11 is opened, so that there is enough cleaning liquid in the flow chamber 20, and then the sample supply device 30 The sample is replaced with cleaning solution, and then the first pump body 11 is closed. Open the second control valve 102 and the third control valve 103, open the second pump body 12, and make the second pump body 12 run in reverse, so as to circulate the pipelines and sub-pipelines in the flow chamber 20 to dredge the flow chamber 20 Then the second pump body 12 rotates forward to discharge the waste liquid to the waste liquid pool 60, so as to clear the pipeline in the flow chamber 20.

Referring to FIG. 1 , a first damper 81 is further provided between the first pressure sensor 51 and the first pump body 11 , and a first damper 81 is further provided between the second pressure sensor 52 and the second pump body 12 . The second damper 82 . The first damper 81 and the second damper 82 can reduce the pulsation of the liquid flow, so that the flow of the liquid is more stable.

Referring to Fig. 1, the liquid circuit system 100 of the cytometer also includes a third pump body 13 and a fourth pump body 14, the third pump body 13 is provided with two pipelines, one of which is connected to the sheath The liquid container 40 is in communication, and the other pipeline is in communication with the cleaning liquid pool 70;

The fourth pump body 14 communicates with the waste liquid pool 60 through a pipeline.

Wherein, the third pump body 13 and the fourth pump body 14 can be diaphragm pumps.

Through the third pump body 13 and the fourth pump body 14, when the liquid flow is unstable, the third pump body 13 and the fourth pump body 14 can be adjusted, and the third pump body 13 can control the sheath fluid container 40 and cleaning The stability of the pressure in the liquid pool 70 means that the pressure at the liquid inlet is stable. The fourth pump body 14 can control the stability of the pressure in the waste liquid tank 60, that is, the pressure at the liquid outlet is stable.

Optionally, a first air pressure sensor 41 and a first hydraulic pressure sensor 42 are provided in the sheath fluid container 40, a second air pressure sensor 71 and a second hydraulic pressure sensor 72 are provided in the cleaning liquid pool 70, and the waste liquid The tank 60 is provided with a third air pressure sensor 61 and a third hydraulic pressure sensor 62 .

Through the above settings, the sheath fluid container 40 , the cleaning fluid pool 70 , and the waste fluid pool 60 can be accurately monitored to regulate the third pump body 13 and the fourth pump body 14 according to the air pressure value and hydraulic pressure value.

The present invention also provides a cytometer, including a controller and a fluid circuit system 100 of the cytometer, the controller is electrically connected to the first pump body 11 and the second pump body 12 of the fluid circuit system 100 of the cytometer .

The first air pressure sensor 41 , the first hydraulic pressure sensor 42 , the second air pressure sensor 71 , the second hydraulic pressure sensor 72 , the third air pressure sensor 61 and the third hydraulic pressure sensor 62 are all electrically connected to the controller.

Referring to Fig. 3, through the setting of the controller, intelligent control can be realized, and the control of the cytometer can be realized through the preset threshold. Specifically, the first sensor 51 and the second sensor 52 transmit the air pressure value to the controller, and the controller judges and compares the air pressure value of the first sensor 51 and the second sensor 52 with the preset threshold value. The information adjusts the opening and closing and operating speeds of the first pump body 11 and the second pump body 12 .

The above is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. Under the inventive concept of the present invention, the equivalent structural transformation made by using the description of the present invention and the contents of the accompanying drawings, or direct/indirect use All other relevant technical fields are included in the patent protection scope of the present invention.

Claims (10)

1. A liquid circuit system of a cytometer, comprising a first pump body, a second pump body, a flow chamber, a sample providing device, and a sheath fluid container, One end of the first pump body communicates with the flow chamber, and the other end communicates with the sheath fluid container, for pumping the sheath fluid in the sheath fluid container into the flow chamber; The first end of the flow chamber communicates with the first pump body, the second end communicates with the second pump body, a pressure difference is formed between the first pump body and the second pump body, and the A sample providing device communicates with the flow chamber for providing sample liquid to the flow chamber according to the pressure difference. 2. The liquid circuit system of the cytometer according to claim 1, wherein a first pressure sensor is arranged between the first pump body and the flow chamber, and the flow chamber and the second pump A second pressure sensor is provided between the bodies. 3. The liquid circuit system of the cytometer according to claim 1, wherein the first pump body and the second pump body are peristaltic pumps. 4. The liquid circuit system of the cytometer according to claim 1, characterized in that, the liquid circuit system of the cytometer further comprises a waste liquid pool, and the waste liquid pool is communicated with the second pump body. 5. The liquid circuit system of the cytometer as claimed in claim 1, characterized in that, the liquid circuit system of the cytometer further comprises a cleaning liquid pool, the cleaning liquid pool is filled with cleaning liquid, and the cleaning liquid is The pool communicates with the first pump body, and a first control valve is provided between the cleaning liquid pool and the first pump body. 6. The liquid circuit system of the cytometer as claimed in claim 5, wherein a second control valve is arranged on the main line between the second pump body and the flow chamber, and the second pump body There is also a branch pipeline between the flow chamber and a third control valve on the branch pipeline. 7. The liquid circuit system of the cytometer as claimed in claim 5, wherein the liquid circuit system of the cytometer further comprises a third pump body and a fourth pump body, and the third pump body is provided with two Pipelines, one of which is in communication with the sheath fluid container, and the other of the pipelines is in communication with the cleaning solution pool; The fourth pump body communicates with the waste liquid pool through a pipeline. 8. The liquid circuit system of the cytometer according to claim 7, wherein a first air pressure sensor and a first hydraulic pressure sensor are arranged in the sheath fluid container, and a second air pressure sensor and a second air pressure sensor are arranged in the cleaning liquid pool. As for the second hydraulic pressure sensor, a third air pressure sensor and a third hydraulic pressure sensor are arranged in the waste liquid pool. 9. The liquid circuit system of the cytometer according to any one of claims 1 to 8, wherein a first damper is further provided between the first pressure sensor and the first pump body, and the first pressure sensor A second damper is also provided between the second pressure sensor and the second pump body. 10. A cytometer, characterized in that it comprises a controller and the fluid circuit system of the cytometer according to any one of claims 1 to 9, the controller and the first pump of the fluid circuit system of the cytometer body and the second pump body are electrically connected.