CN105801191A - Effective microbiota strengthened domestic garbage composting technology - Google Patents
Effective microbiota strengthened domestic garbage composting technology Download PDFInfo
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Abstract
本发明公开了一种有效微生物群强化生活垃圾堆肥工艺,堆肥前将塑料、金属、玻璃、纸张等可回收利用的物质进行分选后,剩余的可降解的有机物质混合添加菌种后进行堆肥,堆肥体积按长×宽×高=4m×2.2m×1.85m,水分控制在60%左右;每隔24小时进行温度测定,测定温度时采用多点温度测定方式进行测定;与现有技术相比,本发明有效的提高了垃圾处理的降解率,过程简单,无有毒有害物质产生,降解快速,施工方便,菌种采用垃圾提取,更加节省成本和环保,具有推广使用的价值。The invention discloses an effective microbiota-enhanced domestic waste composting process. Before composting, recyclable materials such as plastics, metals, glass, and papers are sorted, and the remaining degradable organic materials are mixed and added with strains before composting. , the volume of the compost is by length * width * height = 4m * 2.2m * 1.85m, and the moisture is controlled at about 60%; every 24 hours, the temperature measurement is carried out, and the multi-point temperature measurement method is used to measure the temperature; Compared with that, the invention effectively improves the degradation rate of garbage treatment, the process is simple, no toxic and harmful substances are produced, the degradation is fast, the construction is convenient, and the bacteria are extracted by garbage, which is more cost-saving and environmentally friendly, and has the value of popularization and use.
Description
技术领域technical field
本发明涉及一种生物发酵环保技术,尤其涉及一种有效微生物群强化生活垃圾堆肥工艺。The invention relates to a bio-fermentation environmental protection technology, in particular to a process for strengthening domestic garbage composting with effective microbial groups.
背景技术Background technique
堆肥过程主要是利用微生物促进垃圾中可生物降解的有机物向稳定的腐殖质生化转化过程,其中较难降解的物质是纤维素,最难降解的是木质素,所以纤维素、木质素的破坏意味着细胞物质的解体和腐殖质的产生,是堆肥腐熟过程中的最重要的物理性状变化。因此,利用人工接种高效降解菌来促进纤维素、木质素分解的技术,对加速堆肥发酵进程有重要意义。因此,该方面的研究也是垃圾生化处理领域的热点和难点。The composting process is mainly to use microorganisms to promote the biochemical conversion process of biodegradable organic matter in garbage to stable humus. Among them, the most difficult to degrade is cellulose, and the most difficult to degrade is lignin, so the destruction of cellulose and lignin means The disintegration of cell matter and the production of humus are the most important changes in physical properties during the composting process. Therefore, the technology of artificially inoculating high-efficiency degrading bacteria to promote the decomposition of cellulose and lignin is of great significance to accelerate the process of composting fermentation. Therefore, research in this area is also a hot spot and difficulty in the field of waste biochemical treatment.
发明内容Contents of the invention
本发明的目的就在于为了解决上述问题而提供一种有效微生物群强化生活垃圾堆肥工艺。The object of the present invention is to provide a kind of effective microbiota strengthening domestic garbage composting process in order to solve the above problems.
本发明通过以下技术方案来实现上述目的:The present invention achieves the above object through the following technical solutions:
本发明包括以下步骤:The present invention comprises the following steps:
(1)堆肥前将塑料、金属、玻璃、纸张等可回收利用的物质进行分选后,剩余的可降解的有机物质混合添加菌种后进行堆肥,堆肥体积按长×宽×高=4m×2.2m×1.85m,水分控制在60%左右;(1) After sorting plastic, metal, glass, paper and other recyclable substances before composting, the remaining degradable organic substances are mixed with bacteria and then composted. The volume of compost is length × width × height = 4m × 2.2m×1.85m, the moisture is controlled at about 60%;
(2)每隔24小时进行温度测定,测定温度时采用多点温度测定方式进行测定;(2) The temperature is measured every 24 hours, and the multi-point temperature measurement method is used to measure the temperature;
(3)pH测定,鲜样:去离子水=1:10,震荡1h,过滤,25℃测定;(3) pH measurement, fresh sample: deionized water = 1:10, shake for 1 hour, filter, and measure at 25°C;
(4)EC测定,鲜样:去离子水=1:10,震荡1h,过滤,25℃测定;(4) EC determination, fresh sample: deionized water = 1:10, shake for 1 hour, filter, and measure at 25°C;
(5)E4/E6测定,按鲜样:去离子水=1:10称取,震荡16h,离心,过滤,过滤液在波长465nm、665nm分别测定吸光度值;(5) Determination of E4/E6, according to fresh sample: deionized water = 1:10, weighed, shaken for 16 hours, centrifuged, filtered, and the absorbance values of the filtrate were measured at wavelengths of 465nm and 665nm respectively;
含水率测定,鲜样称取,105℃干燥4h,称量,含水率=(M鲜-M干)/M鲜;Determination of moisture content, fresh samples were weighed, dried at 105°C for 4 hours, weighed, moisture content = (M fresh - M dry)/M fresh;
具体地,所述菌种从堆肥升温阶段的分离菌种及实验室保藏菌种,分别接种于生活垃圾、猪粪和青稞秸秆的堆肥浸出汁培养液摇瓶,于160rpm培养48h,于4000rpm离心5min,收集上清液,测定上清液中TOC的含量,以TOC的降解率为指标,进行有机质分解菌种的初筛而得。Specifically, the strains were inoculated from the isolated strains in the compost heating stage and the strains preserved in the laboratory, respectively, in shake flasks of compost leach juice culture solution of domestic garbage, pig manure, and highland barley straw, cultured at 160rpm for 48h, and centrifuged at 4000rpm. After 5 minutes, collect the supernatant, measure the content of TOC in the supernatant, and use the degradation rate of TOC as an index to carry out the primary screening of organic matter decomposing bacteria.
本发明的有益效果在于:The beneficial effects of the present invention are:
本发明是一种有效微生物群强化生活垃圾堆肥工艺,与现有技术相比,本发明有效的提高了垃圾处理的降解率,过程简单,无有毒有害物质产生,降解快速,施工方便,菌种采用垃圾提取,更加节省成本和环保,具有推广使用的价值。The present invention is an effective microbiota-enhanced domestic waste composting process. Compared with the prior art, the present invention effectively improves the degradation rate of waste treatment, the process is simple, no toxic and harmful substances are produced, the degradation is fast, the construction is convenient, and the strains The use of garbage extraction is more cost-saving and environmentally friendly, and has the value of popularization and use.
具体实施方式detailed description
下面对本发明作进一步说明:The present invention will be further described below:
本发明包括以下步骤:The present invention comprises the following steps:
(6)堆肥前将塑料、金属、玻璃、纸张等可回收利用的物质进行分选后,剩余的可降解的有机物质混合添加菌种后进行堆肥,堆肥体积按长×宽×高=4m×2.2m×1.85m,水分控制在60%左右;(6) After sorting plastic, metal, glass, paper and other recyclable materials before composting, the remaining degradable organic materials are mixed with bacteria and then composted. The volume of compost is length×width×height=4m× 2.2m×1.85m, the moisture is controlled at about 60%;
(7)每隔24小时进行温度测定,测定温度时采用多点温度测定方式进行测定;(7) The temperature is measured every 24 hours, and the multi-point temperature measurement method is used to measure the temperature;
(8)pH测定,鲜样:去离子水=1:10,震荡1h,过滤,25℃测定;(8) pH measurement, fresh sample: deionized water = 1:10, shake for 1 hour, filter, and measure at 25°C;
(9)EC测定,鲜样:去离子水=1:10,震荡1h,过滤,25℃测定;(9) EC determination, fresh sample: deionized water = 1:10, shake for 1 hour, filter, and measure at 25°C;
(10)E4/E6测定,按鲜样:去离子水=1:10称取,震荡16h,离心,过滤,过滤液在波长465nm、665nm分别测定吸光度值;(10) Determination of E4/E6, weigh fresh sample: deionized water = 1:10, shake for 16 hours, centrifuge, filter, and measure the absorbance value of the filtrate at wavelengths of 465nm and 665nm respectively;
含水率测定,鲜样称取,105℃干燥4h,称量,含水率=(M鲜-M干)/M鲜;Determination of moisture content, fresh samples were weighed, dried at 105°C for 4 hours, weighed, moisture content = (M fresh - M dry)/M fresh;
具体地,所述菌种从堆肥升温阶段的分离菌种及实验室保藏菌种,分别接种于生活垃圾、猪粪和青稞秸秆的堆肥浸出汁培养液摇瓶,于160rpm培养48h,于4000rpm离心5min,收集上清液,测定上清液中TOC的含量,以TOC的降解率为指标,进行有机质分解菌种的初筛而得。Specifically, the strains were inoculated from the isolated strains of the compost heating stage and the strains preserved in the laboratory, respectively, in shake flasks of compost leach juice culture liquid of domestic garbage, pig manure, and highland barley straw, cultured at 160rpm for 48h, and centrifuged at 4000rpm. After 5 minutes, collect the supernatant, measure the content of TOC in the supernatant, and use the degradation rate of TOC as an index to carry out the primary screening of organic matter decomposing bacteria.
以上显示和描述了本发明的基本原理和主要特征及本发明的优点。本行业的技术人员应该了解,本发明不受上述实施例的限制,上述实施例和说明书中描述的只是说明本发明的原理,在不脱离本发明精神和范围的前提下,本发明还会有各种变化和改进,这些变化和改进都落入要求保护的本发明范围内。本发明要求保护范围由所附的权利要求书及其等效物界定。The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements all fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.
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Citations (9)
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CN1355153A (en) * | 2000-11-23 | 2002-06-26 | 威海哈尔滨工业大学科学与工程研究院 | Composite organic fertilizer made of life garbage and its preparing process |
CN1654434A (en) * | 2004-02-13 | 2005-08-17 | 薛占何 | Urban domestic garbage comprehensive treatment technology |
CN1840511A (en) * | 2005-03-29 | 2006-10-04 | 中国农业大学 | Disposal method of domestic garbage and its special fermentation bin |
CN101200384A (en) * | 2007-10-19 | 2008-06-18 | 天津师范大学 | A method for controlling heavy metal pollution by particle size sieving of domestic waste composting |
CN101580412A (en) * | 2009-06-26 | 2009-11-18 | 哈尔滨工业大学 | Low-temperature aerobic composting method for organic solid waste |
CN103360131A (en) * | 2013-07-18 | 2013-10-23 | 句容市和春园农业机械专业合作社 | Vegetable waste composting technology |
CN103787699A (en) * | 2014-02-14 | 2014-05-14 | 青岛吉曼新能源技术有限公司 | Treatment method and treatment device of town household garbage |
CN104370582A (en) * | 2014-11-04 | 2015-02-25 | 四川联星环保科技有限公司 | Organic garbage odorless aerobic composting method |
CN104475421A (en) * | 2014-11-13 | 2015-04-01 | 青岛市环境卫生科研所 | Domestic waste landfill reservoir area recycling technology |
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- 2016-03-09 CN CN201610132809.9A patent/CN105801191A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1355153A (en) * | 2000-11-23 | 2002-06-26 | 威海哈尔滨工业大学科学与工程研究院 | Composite organic fertilizer made of life garbage and its preparing process |
CN1654434A (en) * | 2004-02-13 | 2005-08-17 | 薛占何 | Urban domestic garbage comprehensive treatment technology |
CN1840511A (en) * | 2005-03-29 | 2006-10-04 | 中国农业大学 | Disposal method of domestic garbage and its special fermentation bin |
CN101200384A (en) * | 2007-10-19 | 2008-06-18 | 天津师范大学 | A method for controlling heavy metal pollution by particle size sieving of domestic waste composting |
CN101580412A (en) * | 2009-06-26 | 2009-11-18 | 哈尔滨工业大学 | Low-temperature aerobic composting method for organic solid waste |
CN103360131A (en) * | 2013-07-18 | 2013-10-23 | 句容市和春园农业机械专业合作社 | Vegetable waste composting technology |
CN103787699A (en) * | 2014-02-14 | 2014-05-14 | 青岛吉曼新能源技术有限公司 | Treatment method and treatment device of town household garbage |
CN104370582A (en) * | 2014-11-04 | 2015-02-25 | 四川联星环保科技有限公司 | Organic garbage odorless aerobic composting method |
CN104475421A (en) * | 2014-11-13 | 2015-04-01 | 青岛市环境卫生科研所 | Domestic waste landfill reservoir area recycling technology |
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