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CN105794769A - Special diluting liquid for bovine seminal plasma - Google Patents

Special diluting liquid for bovine seminal plasma Download PDF

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Publication number
CN105794769A
CN105794769A CN201610254552.4A CN201610254552A CN105794769A CN 105794769 A CN105794769 A CN 105794769A CN 201610254552 A CN201610254552 A CN 201610254552A CN 105794769 A CN105794769 A CN 105794769A
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grams
diluent
solvent
special
gram
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李志新
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/124—Disinfecting agents, e.g. antimicrobials
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

本发明公开了一种牛精液专用稀释液，以灭菌蒸馏水为溶剂，每1000ml稀释液由以下成分组成：甘油5‑10克、大豆卵磷脂1‑2克、海藻糖8‑12克、维生素E2‑4克、卵黄10‑15克、柠檬酸三钠4‑7克、青霉素80‑100万IU、其余为溶剂，调节PH值为6.6‑7.0。利用本发明的稀释液对牛精液进行冷冻保存，解冻后精子活力达75％以上。The invention discloses a special diluent for bovine semen, which uses sterilized distilled water as a solvent, and every 1000ml of the diluent consists of the following components: 5-10 grams of glycerin, 1-2 grams of soybean lecithin, 8-12 grams of trehalose, vitamin E2-4 grams, 10-15 grams of egg yolk, 4-7 grams of trisodium citrate, 80-1 million IU of penicillin, and the rest are solvents, and the pH value is adjusted to 6.6-7.0. The bovine semen is cryopreserved by using the diluent of the present invention, and the motility of the sperm reaches over 75% after thawing.

Description

一种牛精液专用稀释液A special diluent for bovine semen

技术领域technical field

本发明涉及生物技术领域，具体涉及一种牛精液专用稀释液。The invention relates to the field of biotechnology, in particular to a special diluent for bovine semen.

背景技术Background technique

家畜精液保存技术是二十世纪随着人工授精技术广泛应用而生产的，原理是通过降低或抑制精子新陈代谢程度达到延长精子在体外寿命的目的。为此，研究者试验将精液放在低温状态(非结冰)或冷冻(结冰)状态下保存。但在温度变化过程中，精液对精子的保护能力是有限的。为此，进行精液冷冻保存就要配合使用专用的溶液先将精液进行稀释。对稀释液的基本要求包括pH值、缓冲性、渗透压和低温保护性能。Livestock semen preservation technology was produced with the widespread application of artificial insemination technology in the 20th century. The principle is to prolong the life of sperm in vitro by reducing or inhibiting the degree of sperm metabolism. To this end, the researchers experimented with storing semen in a cryogenic (non-frozen) or frozen (frozen) state. However, during temperature changes, semen's ability to protect sperm is limited. For this reason, semen cryopreservation requires the use of a special solution to dilute the semen first. Basic requirements for diluents include pH, buffering, osmotic pressure, and cryoprotective properties.

发明内容Contents of the invention

本发明要解决的技术问题是提供一种牛精液专用稀释液。The technical problem to be solved by the present invention is to provide a special diluent for bovine semen.

解决上述技术问题，本发明采用的技术方案是：To solve the problems of the technologies described above, the technical solution adopted in the present invention is:

一种牛精液专用稀释液，以灭菌蒸馏水为溶剂，每1000ml稀释液由以下成分组成：甘油5-10克、大豆卵磷脂1-2克、海藻糖8-12克、维生素E2-4克、卵黄10-15克、柠檬酸三钠4-7克、青霉素80-100万IU、其余为溶剂，调节PH值为6.6-7.0。A special diluent for bovine semen, using sterilized distilled water as a solvent, and every 1000ml of the diluent consists of the following components: 5-10 grams of glycerin, 1-2 grams of soybean lecithin, 8-12 grams of trehalose, and 2-4 grams of vitamin E , egg yolk 10-15 grams, trisodium citrate 4-7 grams, penicillin 800,000-1,000,000 IU, and the rest are solvents, and the pH value is adjusted to 6.6-7.0.

优选地，一种牛精液专用稀释液，以灭菌蒸馏水为溶剂，每1000ml稀释液由以下成分组成：甘油7.5克、大豆卵磷脂1.5克、海藻糖10克、维生素E3克、卵黄12.5克、柠檬酸三钠5.5克、青霉素90万IU、其余为溶剂，调节PH值为6.8。Preferably, a special diluent for bovine semen, using sterilized distilled water as a solvent, consists of the following components per 1000ml of diluent: 7.5 grams of glycerin, 1.5 grams of soybean lecithin, 10 grams of trehalose, 3 grams of vitamin E, 12.5 grams of egg yolk, 5.5 grams of trisodium citrate, 900,000 IU of penicillin, and the rest are solvents, and the pH value is adjusted to 6.8.

本发明的优点是：The advantages of the present invention are:

1、甘油能渗入细胞内，浓缩或结合胞内水分，而且甘油也有稀释作用，能降低溶液中盐的浓度和冷冻液的渗透压；大豆卵磷脂和卵黄对正常顶体的保持有促进作用；海藻糖和维生素E能提高活动精子的百分率和正常顶体的比例；柠檬酸钠用以保持精液适当的pH值；青霉素等抗菌物质抑制细菌微生物的孳生。1. Glycerin can penetrate into cells, concentrate or combine intracellular water, and glycerin also has a diluting effect, which can reduce the concentration of salt in the solution and the osmotic pressure of the freezing liquid; soybean lecithin and egg yolk can promote the maintenance of normal acrosomes; Trehalose and vitamin E can increase the percentage of motile sperm and the ratio of normal acrosomes; sodium citrate is used to maintain the proper pH value of semen; antibacterial substances such as penicillin inhibit the growth of bacterial microorganisms.

2、利用本发明的稀释液对牛精液进行冷冻保存，解冻后精子活力达75％以上。2. Utilize the diluent of the present invention to carry out cryopreservation to the bovine semen, and the motility of the sperm can reach more than 75% after thawing.

具体实施方式detailed description

下面对本发明的实施例进行详细阐述，以使本发明的优点和特征能更易于被本领域技术人员理解，从而对本发明的保护范围做出更为清楚明确的界定。The embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

实施例1Example 1

一种牛精液专用稀释液，以灭菌蒸馏水为溶剂，每1000ml稀释液由以下成分组成：甘油5克、大豆卵磷脂1克、海藻糖8克、维生素E2克、卵黄10克、柠檬酸三钠4克、青霉素80万IU、其余为溶剂，调节PH值为6.6。A special diluent for bovine semen, using sterilized distilled water as a solvent, and every 1000ml of the diluent consists of the following ingredients: 5 grams of glycerin, 1 gram of soybean lecithin, 8 grams of trehalose, 2 grams of vitamin E, 10 grams of egg yolk, three citric acids 4 grams of sodium, 800,000 IU of penicillin, and the rest are solvents, and the pH value is adjusted to 6.6.

实施例2Example 2

一种牛精液专用稀释液，以灭菌蒸馏水为溶剂，每1000ml稀释液由以下成分组成：甘油7.5克、大豆卵磷脂1.5克、海藻糖10克、维生素E3克、卵黄12.5克、柠檬酸三钠5.5克、青霉素90万IU、其余为溶剂，调节PH值为6.8。A special diluent for bovine semen, using sterilized distilled water as a solvent, and each 1000ml diluent is composed of the following components: 7.5 grams of glycerin, 1.5 grams of soybean lecithin, 10 grams of trehalose, 3 grams of vitamin E, 12.5 grams of egg yolk, tricitric acid 5.5 grams of sodium, 900,000 IU of penicillin, and the rest are solvents, and the pH value is adjusted to 6.8.

实施例3Example 3

一种牛精液专用稀释液，以灭菌蒸馏水为溶剂，每1000ml稀释液由以下成分组成：甘油10克、大豆卵磷脂2克、海藻糖12克、维生素E4克、卵黄15克、柠檬酸三钠7克、青霉素100万IU、其余为溶剂，调节PH值为7.0。A special diluent for bovine semen, using sterilized distilled water as a solvent, and every 1000ml of the diluent consists of the following ingredients: 10 grams of glycerin, 2 grams of soybean lecithin, 12 grams of trehalose, 4 grams of vitamin E, 15 grams of egg yolk, three citric acids 7 grams of sodium, 1,000,000 IU of penicillin, and the rest are solvents, and the pH value is adjusted to 7.0.

Claims (2)

1. the special diluent of cattle seminal fluid, it is characterised in that with sterile purified water as solvent, Every 1000ml diluent consists of the following composition: glycerol 5-10 gram, soybean lecithin 1-2 gram, Trehalose 8-12 gram, vitamin E2-4 grams, 10-15 gram of yolk, trisodium citrate 4-7 gram, Penicillin 80-100 ten thousand IU, remaining be solvent, regulation pH value is 6.6-7.0.
The special diluent of cattle seminal fluid the most according to claim 1, it is characterised in that to go out Bacterium distilled water is solvent, and every 1000ml diluent consists of the following composition: glycerol 7.5 grams, Soybean lecithin 1.5 grams, trehalose 10 grams, vitamin E 3 grams, 12.5 grams of yolk, lemon Lemon acid trisodium 5.5 grams, penicillin 900,000 IU, remaining be solvent, regulation pH value be 6.8.
CN201610254552.4A 2016-04-22 2016-04-22 Special diluting liquid for bovine seminal plasma Pending CN105794769A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109221085A (en) * 2018-09-25 2019-01-18 上海祥欣畜禽有限公司 The fresh essence of pig saves dilution powder

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101220345A (en) * 2008-01-23 2008-07-16 西北农林科技大学 A kind of bovine frozen semen diluent and preparation method thereof
CN101263806A (en) * 2008-04-11 2008-09-17 高庆华 Dilution for preserving semen under normal temperature
CN101310729A (en) * 2007-05-23 2008-11-26 辽宁省辽宁绒山羊育种中心 Animal origin free flocks and herds frozen semen diluent and production method of flocks and herds frozen semen
CN101554152A (en) * 2009-05-21 2009-10-14 西北农林科技大学 Frozen semen diluent and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101310729A (en) * 2007-05-23 2008-11-26 辽宁省辽宁绒山羊育种中心 Animal origin free flocks and herds frozen semen diluent and production method of flocks and herds frozen semen
CN101220345A (en) * 2008-01-23 2008-07-16 西北农林科技大学 A kind of bovine frozen semen diluent and preparation method thereof
CN101263806A (en) * 2008-04-11 2008-09-17 高庆华 Dilution for preserving semen under normal temperature
CN101554152A (en) * 2009-05-21 2009-10-14 西北农林科技大学 Frozen semen diluent and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109221085A (en) * 2018-09-25 2019-01-18 上海祥欣畜禽有限公司 The fresh essence of pig saves dilution powder

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Application publication date: 20160727