CN105794354B - A kind of sweet photo seed float seedling method - Google Patents
A kind of sweet photo seed float seedling method Download PDFInfo
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- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
本发明涉及一种甘薯实生种子的漂浮育苗方法,属于甘薯实生种子育苗技术领域,具体步骤为:1)建立漂浮育苗池,并灌水;2)选用200孔泡沫漂浮盘,往盘中填入含水量60%的育苗基质;3)挑选98%浓硫酸处理并经过温汤、药剂浸泡处理的已经发芽的甘薯实生种子播种,盖土,抹平,放入育苗池中培养;4)培养期间约15天补充一次水;5)苗齐后3‑5天,施第一次育苗肥,施用量为15g/盘,保持氮的浓度为300mg/L,隔7天追施一次;6)播种后30‑40天,即达到壮苗标准,可进行带土移栽。本发明采用漂浮育苗技术可以促进种子快速、整齐的出苗,出苗率可达100%,3‑4天即可齐苗,并能使实生苗保持生长一致,苗壮、根系发达、根冠比比值高、株高适宜,整个过程省时、省力。
The invention relates to a method for floating seedling raising of sweet potato seeds, which belongs to the technical field of seedling raising of sweet potato seeds. The specific steps are as follows: 1) establishing a floating seedling raising pond and filling it with water; 2) selecting a 200-hole foam floating tray and filling the tray with 60% of the seedling medium; 3) Select 98% concentrated sulfuric acid and germinated sweet potato seeds that have been treated with warm soup and medicament soaking for sowing, covering with soil, smoothing, and putting them into the seedling pond for cultivation; 4) The cultivation period is about 15 5) 3-5 days after the seedlings are ready, apply the seedling fertilizer for the first time, the application rate is 15g/pan, keep the nitrogen concentration at 300mg/L, and topdress once every 7 days; 6) 30 days after sowing After ‑40 days, the seedlings have reached the standard of strong seedlings, and can be transplanted with soil. The present invention adopts the floating seedling raising technology to promote fast and neat emergence of seeds, the emergence rate can reach 100%, and the seedlings can be assembled in 3-4 days, and the seedlings can be maintained to grow consistently, with strong seedlings, well-developed root systems, and root-to-shoot ratio The height and plant height are suitable, and the whole process saves time and effort.
Description
技术领域technical field
本发明属于甘薯实生种子育苗技术领域,具体地说,涉及一种甘薯实生种子漂浮育苗方法。The invention belongs to the technical field of seedling raising of sweet potato seeds, and in particular relates to a method for raising seedlings of sweet potato seeds by floating.
背景技术Background technique
甘薯(Ipomoea batatas Lam.)又名红薯、番薯、地瓜、红苕等,为双子叶植物,旋花科(Convolvulaceae)甘薯属(Ipornoea)的一种蔓生草本、喜温和短日照的一年生或多年生植物。甘薯因产量高、用途广泛和适应性强等优点,在中国是仅次于水稻、小麦和玉米的重要粮食、饲料、能源和加工作物。Sweet potato (Ipomoea batatas Lam.), also known as sweet potato, sweet potato, sweet potato, sweet potato, etc., is a dicotyledonous plant, a vine plant of the genus Ipornoea in the family Convolvulaceae, an annual or perennial plant that likes mild and short sunshine. Sweet potato is an important food, feed, energy and processing crop in China after rice, wheat and corn due to its advantages of high yield, wide range of uses and strong adaptability.
甘薯在热带地方能开花结实,但在温带通常不能开花结实。如在我国中部和北部地区,绝大多数甘薯品种在自然条件下都不能开花,即使在我国南方各省,也并非所有品种都能开花。据烟台地区农业科学研究所甘薯研究室研究甘薯开花、花粉萌发、结实及种子成熟的适宜温度为26℃。故对于大部分地区甘薯传统育种工作者来说,保证甘薯实生种子的高发芽率和高成苗率,是选育高产优质甘薯品种的基础。因此有必要开发一种提高甘薯实生种子发芽率及成苗率的育苗技术。Sweet potatoes can flower and set in tropical places, but usually cannot flower and set in temperate regions. For example, in the central and northern regions of my country, most sweet potato varieties cannot bloom under natural conditions. Even in the southern provinces of my country, not all varieties can bloom. According to the Sweet Potato Research Office of the Yantai Regional Agricultural Science Research Institute, the optimum temperature for sweet potato flowering, pollen germination, fruiting and seed maturity is 26 °C. Therefore, for traditional sweet potato breeders in most areas, ensuring high germination rate and high seedling rate of sweet potato seeds is the basis for breeding high-yield and high-quality sweet potato varieties. Therefore it is necessary to develop a seedling raising technique that improves the germination rate and seedling rate of sweet potato seeds.
《广东农业科学》2003年第5期公开了“浓硫酸处理甘薯种子的发芽试验”表明用浓硫酸浸泡甘薯种子20-40分钟,能够使发芽率达到96%以上,比传统方法省时省力。公开号为CN103250547A公开了冬季马铃薯实生种子日光温室育苗移栽的方法,公开号为CN202310796U公开了用于马铃薯实生种子栽培的育苗装置,公开号为CN103039245A公开了一种马铃薯实生籽设施育苗的方法,公开号CN103828699A公开了一种烟苗漂浮育苗的改良方法,公开号为CN101627728公开了一种利用甘薯实生种子培育无病无毒试管苗的方法,公开号为CN101897294A公开了甘薯实生苗一年两代选种方法。目前,甘薯实生种子育苗的常用方法为:在温室中或露天地方用草炭土、蛭石粉或育苗基质建立一个苗床,并在苗床上播种种子,浇适量水,然后在苗床上搭建小拱棚,此种方法较为费工、费时,并且苗生长不齐、苗弱,移栽成活率不高。《南方农业学报》2011年第4期公开了甘薯藤蔓的漂浮育苗快繁技术,而关于甘薯实生种子的漂浮育苗技术目前尚未见报道。"Guangdong Agricultural Science" published the "Germination Test of Sweet Potato Seeds Treated with Concentrated Sulfuric Acid" in the 5th issue of 2003, showing that soaking sweet potato seeds with concentrated sulfuric acid for 20-40 minutes can make the germination rate reach more than 96%, saving time and labor compared with traditional methods. Publication number is that CN103250547A discloses the method for transplanting seedlings of potato seedlings in sunlight greenhouse in winter, and publication number is that CN202310796U discloses the seedling raising device that is used for potato seedling cultivation, and publication number is that CN103039245A discloses a kind of method for planting seedlings of potato seedlings, Publication No. CN103828699A discloses an improved method for floating seedlings of tobacco seedlings. Publication No. CN101627728 discloses a method for cultivating disease-free and non-toxic test-tube seedlings using sweet potato seeds. Publication No. CN101897294A discloses two generations of sweet potato seedlings a year. Selection method. At present, the common method of growing sweet potato seed seedlings is: set up a seedbed with peat soil, vermiculite powder or seedling substrate in the greenhouse or in the open air, and sow seeds on the seedbed, pour appropriate amount of water, and then build a small shed on the seedbed. This method is more labor-intensive, time-consuming, and the seedling growth is uneven, the seedling is weak, and the survival rate of transplanting is not high. "Southern Agricultural Journal" 2011 No. 4 issue discloses the floating seedling raising technology of sweet potato vines, but there is no report about the floating seedling raising technology of sweet potato seedlings.
发明内容Contents of the invention
为了克服背景技术中存在的问题,本发明提供了一种甘薯实生种子漂浮育苗方法,能提高成苗率,出苗整齐性好,漂浮育苗的更加经济、环保,而且能最大限度的控制病虫草害,经本发明方法育出来的实生苗茎秆粗壮、根系发达、叶面积大、根冠比比值大、株高适宜,能增加移栽成活率。In order to overcome the problems existing in the background technology, the present invention provides a method for floating seedling cultivation of sweet potato seeds, which can increase the seedling rate, have good seedling emergence uniformity, and the floating seedling cultivation is more economical and environmentally friendly, and can control diseases, insect pests and weeds to the greatest extent The seedlings cultivated by the method of the invention have thick stems, well-developed root systems, large leaf area, large root-to-shoot ratio, suitable plant height, and can increase the survival rate of transplanting.
为实现上述目的,本发明是通过如下技术方案实现的:To achieve the above object, the present invention is achieved through the following technical solutions:
一种甘薯实生种子漂浮育苗方法,具体的步骤为:A method for floating seedling raising of sweet potato seeds, the specific steps are:
1)建育苗池:用小红砖搭建池子,在池子底部铺上一层塑料膜,并且池子上方活动罩设有一个用钢架焊接而成的拱棚,拱棚外围用厚塑料薄膜封好,拱棚两端设置有可自由掀开的厚塑料薄膜,待苗池建好后,往池子中注入自来水,水位不漫过池子;1) Build a nursery pool: build a pool with small red bricks, spread a layer of plastic film on the bottom of the pool, and set a shed welded with a steel frame on the movable cover above the pool. Both ends are provided with thick plastic films that can be opened freely. After the seedling pond is built, tap water is poured into the pond, and the water level does not overflow the pond;
2)漂浮育苗盘准备:选用200孔聚苯乙烯泡沫塑料盘及与之配套的播种器;2) Preparation of floating seedling trays: select 200-hole polystyrene foam trays and matching seeders;
3)育苗基质:沼渣晒干、碾碎、过20目筛,备用;肥沃的园土,去除杂物,晒干,碾碎,过20目筛,备用;将沼渣、蛭石、园土、草木灰混合配制成育苗基质,使用前先加水拌湿,基质含水量为60%,即手捏成团,放下呈散开的状态;3) Seedling-raising substrate: biogas residues are dried in the sun, crushed, passed through a 20-mesh sieve, and set aside; the fertile garden soil is removed from debris, dried, crushed, passed through a 20-mesh sieve, and set aside; biogas residues, vermiculite, garden Soil and plant ash are mixed to make a seedling substrate, add water and mix it wet before use, the moisture content of the substrate is 60%, that is, knead it into a ball by hand, and put it down to form a loose state;
4)实生种子播种前处理:将甘薯实生种子用98%浓硫酸浸泡30分钟,且不断搅拌,使种子充分接触浓硫酸,将浓硫酸倒出并用自来水将种子冲洗3遍以上,保证无浓硫酸残留,然后转入50-55℃温水中浸泡,此过程要持续搅拌,使种子受热均匀,10-15分钟后温度降至30℃时,停止搅拌,将种子转入25%多菌灵可湿性粉剂250倍液中,继续浸泡5-10分钟,取出种子用蒸馏水洗净,转入培养皿中,加入蒸馏水,蒸馏水以刚好浸没种子为宜,将培养皿的盖子盖好,放入2光照培养箱中光照培养;4) Pre-sowing treatment of seeds: Soak the seeds of sweet potato with 98% concentrated sulfuric acid for 30 minutes, and keep stirring, so that the seeds are fully exposed to concentrated sulfuric acid, pour out the concentrated sulfuric acid and rinse the seeds with tap water for more than 3 times to ensure that there is no concentrated sulfuric acid After 10-15 minutes, when the temperature drops to 30°C, stop stirring and turn the seeds into 25% carbendazim wettable In 250 times of powder solution, continue to soak for 5-10 minutes, take out the seeds and wash them with distilled water, transfer them to a petri dish, add distilled water, the distilled water should just submerge the seeds, cover the lid of the petri dish, and put it in 2 light for cultivation Illumination culture in the box;
5)种子播种:将育苗基质装填在漂浮育苗盘中,然后用配套的播种器在育苗盘上压出1.5cm深的小孔,一次性完成整盘的打孔工作,再用镊子将发芽的种子夹到孔中,使根朝下,上方均匀撒上一层育苗基质,抹平,最后放入育苗池中培养;5) Seed sowing: Fill the seedling raising substrate in the floating seedling raising tray, and then use the supporting seeder to press out 1.5cm deep holes on the seedling raising tray, complete the drilling work of the whole tray at one time, and then use tweezers to germinate the seedlings. The seeds are clamped into the hole, with the roots facing down, and a layer of seedling substrate is evenly sprinkled on the top, smoothed, and finally put into the seedling pond for cultivation;
6)日常管理:在生长期间,观察育苗池中水的深浅变化,不定时向池中加水保证池子中有充足的水,15-20天补充一次水;齐苗后3-5天,按15g/盘的量施入育苗肥,每隔7天施一次肥,播种30-40天后,待苗长到8-10cm,进行移栽。6) Daily management: During the growth period, observe the depth of water in the nursery pond, add water to the pond from time to time to ensure that there is sufficient water in the pond, and replenish water once every 15-20 days; The amount per plate is applied to the seedling fertilizer, and the fertilizer is applied once every 7 days. After 30-40 days of sowing, the seedlings grow to 8-10cm before transplanting.
作为优选,在步骤1)中,池子的大小为长×宽×高=330cm×70cm×10cm,拱棚的规格为长×宽×高=370cm×90cm×80cm。Preferably, in step 1), the size of the pond is length×width×height=330cm×70cm×10cm, and the specification of the shed is length×width×height=370cm×90cm×80cm.
作为优选,在步骤2)中,所述的漂浮育苗盘的规格为长×宽×高=66cm×33.5cm×5.5cm。Preferably, in step 2), the specification of the floating seedling tray is length×width×height=66cm×33.5cm×5.5cm.
作为优选,在步骤3)中,所述的育苗基质按沼渣:蛭石:园土:草木灰=6:2:20:1的重量比制成,且用高压灭菌锅121℃灭菌40分钟,充分杀灭病菌、虫卵、杂草种子。As a preference, in step 3), the seedling-raising substrate is made according to the weight ratio of biogas residue: vermiculite: garden soil: plant ash=6:2:20:1, and is sterilized at 121° C. in an autoclave for 40 Minutes, fully kill germs, eggs, weed seeds.
作为优选,在步骤4)中,甘薯实生种子在光照培养箱中25℃光照培养48h,24h换一次水。Preferably, in step 4), the sweet potato seeds are light-cultured at 25° C. for 48 hours in a light incubator, and the water is changed once every 24 hours.
作为优选,在步骤6)中,所述的育苗肥为N:P2O5:K2O=20:7:13的复合肥,氮浓度控制在300mg/L。Preferably, in step 6), the seedling fertilizer is a compound fertilizer of N:P 2 O 5 :K 2 O=20:7:13, and the nitrogen concentration is controlled at 300mg/L.
本发明的有益效果:Beneficial effects of the present invention:
(1)本发明用浓硫酸腐蚀种子表面坚硬的角质层,结合温汤浸种和药剂浸种,发芽率可达92.55%以上,可打破休眠,显著减少病害,提高实生苗的病害提抗力,为提高育种效率和良好单株的入选几率打下坚实的基础,出苗整齐性比传统育苗要好;(1) The present invention corrodes the hard cuticle of the seed surface with concentrated sulfuric acid, and combines soaking seeds in warm soup and medicament soaking seeds, and the germination rate can reach more than 92.55%, which can break dormancy, significantly reduce diseases, and improve the disease resistance of seedlings. The efficiency and the selection probability of a good single plant have laid a solid foundation, and the uniformity of seedling emergence is better than that of traditional seedling cultivation;
(2)本方法采用漂浮育苗的方法,育苗池搭建灵活方便,可循环利用,一个育苗池可容纳2000粒种子,能节省土地,且所需基质较少,相对于常规的苗床育苗比较经济、环保、整洁、卫生,可以最大限度的控制病虫草害;且育苗池内隔很长一段时间才需要加水,若在缺水的地区推广此育苗方法,可以省去浇水投入的大量工作;(2) This method adopts the method for floating seedlings, and the seedling ponds are flexible and convenient to build, and can be recycled. A seedling pond can accommodate 2000 seeds, which can save land, and the required substrate is less, which is more economical and convenient compared to conventional seedbed cultivation. Environmental protection, cleanliness and sanitation can control diseases, insect pests and weeds to the greatest extent; and the nursery pool needs to be filled with water only after a long period of time. If this method of raising seedlings is promoted in water-deficient areas, it can save a lot of work in watering;
(3)本发明中所述的配套播种器,一次性能完成整个育苗盘的打孔工作,比传统的一个一个的用木棍等其他打孔工具打孔效率高,操作简单,而目前此方法在生产上还比较少见;(3) The supporting seeder described in the present invention can complete the punching work of the whole seedling tray at one time, which is more efficient than traditional punching tools such as wooden sticks one by one, and is simple to operate. It is still relatively rare in production;
(4)本发明中所述的育苗基质为沼渣:蛭石:园土:草木灰=6:2:20:1按重量比配制,并经过高压灭菌,该育苗基质富含有机质和腐殖质,营养丰富,钾元素含量高,吸水性好,保水能力强,很适合甘薯幼苗的生长,并且最大限度的避免了病虫草害的发生;(4) The seedling-raising substrate described in the present invention is biogas residue: vermiculite: garden soil: plant ash=6:2:20:1 is prepared by weight ratio, and through autoclaving, this seedling-raising substrate is rich in organic matter and humus, Rich in nutrients, high in potassium, good in water absorption and strong in water retention, it is very suitable for the growth of sweet potato seedlings, and avoids the occurrence of diseases, insect pests and weeds to the greatest extent;
(5)在本发明方法中,可将复合肥均匀撒施溶解到漂浮池的水中,整个育苗池中的养分非常均匀,能够保证苗生长的整齐性和一致性,不会因为施肥不均或肥料直接和叶面接触而造成烧苗;(5) In the method of the present invention, the compound fertilizer can be uniformly spread and dissolved in the water in the floating pond, and the nutrients in the whole seedling pond are very uniform, which can ensure the uniformity and consistency of seedling growth, and will not be caused by uneven fertilization or The direct contact of fertilizer with the leaf surface causes burnt seedlings;
(6)通过本发明方法育出来的实生苗茎秆粗壮、根系发达,叶面积大、根冠比比值大、株高适宜,其中根长、根数、叶片长宽乘积、根冠比分别比常规苗床育苗增加了26.18%、39.94%、33.43%、76.47%;移栽时能把整个孔穴中的基质一起拿出来,并且根系密集不容易损伤,增加移栽成活率。(6) The seedling stalks bred by the inventive method are thick and strong, the root system is developed, the leaf area is large, the ratio of root to shoot ratio is large, and the plant height is suitable, wherein root length, root number, product of blade length and width, and root to shoot ratio are respectively compared to Seedlings raised in conventional seedbeds increased by 26.18%, 39.94%, 33.43%, and 76.47%. When transplanting, the substrate in the entire hole can be taken out together, and the root system is dense and not easy to damage, which increases the survival rate of transplanting.
附图说明Description of drawings
图1为甘薯实生种子的常规育苗方法(苗床育苗)生长30天的实生苗;Fig. 1 is the seedling that the conventional seedling raising method (seedbed seedling raising) of sweet potato seed grows 30 days;
图2为甘薯实生种子的漂浮育苗方法生长30天的实生苗;Fig. 2 is the seedling that the floating seedling raising method of sweet potato seed grows for 30 days;
图3为苗床育苗甘薯实生苗移栽带土情况;Fig. 3 is the soil situation of transplanting the seedlings of sweet potato seedlings in the seedbed;
图4为漂浮育苗甘薯实生苗移栽带土情况;Fig. 4 is the situation with the soil of transplanting the sweet potato seedling of floating seedling cultivation;
图5为苗床育苗和漂浮育苗培育实生苗生长比较图;Fig. 5 is the growth comparison figure of seedling cultivation in seedbed and floating seedling cultivation;
图6为与漂浮育苗盘配套使用的播种器实物正面图;Fig. 6 is the real front view of the seeder used in conjunction with the floating seedling tray;
图7为与漂浮育苗盘配套使用的播种器实物反面图。Fig. 7 is the actual reverse view of the seeder used in conjunction with the floating seedling tray.
具体实施方式Detailed ways
实施例1Example 1
一种甘薯实生种子漂浮育苗方法,具体的步骤为:A method for floating seedling raising of sweet potato seeds, the specific steps are:
1)建育苗池:用小红砖搭建池子,在池子底部铺上一层塑料膜,并且池子上方活动罩设有一个用钢架焊接而成的拱棚,拱棚外围用厚塑料薄膜封好,拱棚两端设置有可自由掀开的厚塑料薄膜,待苗池建好后,往池子中注入自来水,水位不漫过池子;1) Build a nursery pool: build a pool with small red bricks, spread a layer of plastic film on the bottom of the pool, and set a shed welded with a steel frame on the movable cover above the pool. Both ends are provided with thick plastic films that can be opened freely. After the seedling pond is built, tap water is poured into the pond, and the water level does not overflow the pond;
2)漂浮育苗盘准备:选用200孔聚苯乙烯泡沫塑料盘及与之配套的播种器(如图6、图7所示,能一次性完成打孔过程,节省人力);2) Floating seedling raising tray preparation: select 200 hole polystyrene foam trays and the seeder supporting it (as shown in Figure 6 and Figure 7, the punching process can be completed at one time, saving manpower);
3)育苗基质:沼渣(作物秸秆、牛粪、猪粪等经沼气池发酵后所得,主要成分为有机质和腐殖质等)晒干、碾碎、过20目筛,备用;肥沃的园土,去除杂物,晒干,碾碎,过20目筛,备用;将沼渣、蛭石、园土、草木灰充分混匀制成育苗基质,使用前先加水拌湿,基质含水量为60%,即手捏成团,放下呈散开的状态;3) Seedling-raising substrate: biogas residue (crop straw, cow dung, pig manure, etc. obtained after fermentation in a biogas tank, the main components are organic matter and humus, etc.) dried in the sun, crushed, and passed through a 20-mesh sieve for use; fertile garden soil, Remove debris, dry in the sun, crush, pass through a 20-mesh sieve, and set aside; fully mix biogas residue, vermiculite, garden soil, and plant ash to make a seedling substrate, add water and mix before use, and the moisture content of the substrate is 60%. That is, knead it into a ball with your hands and put it down to spread out;
4)实生种子播种前处理:将甘薯实生种子用98%浓硫酸浸泡30分钟,且不断搅拌,使种子充分接触浓硫酸,将浓硫酸倒出并用自来水将种子冲洗3遍以上,保证无浓硫酸残留,然后转入50-55℃温水中浸泡,边浸泡边搅拌,使种子受热均匀,10-15分钟后温度降至30℃时,停止搅拌,将种子转入25%多菌灵可湿性粉剂250倍液中,继续浸泡5-10分钟,取出种子用蒸馏水洗净,转入培养皿中,加入蒸馏水,蒸馏水以刚好浸没种子为宜,将培养皿的盖子盖好,放入光照培养箱中光照培养至种子露白,统计发芽率;4) Pre-sowing treatment of seeds: Soak the seeds of sweet potato with 98% concentrated sulfuric acid for 30 minutes, and keep stirring, so that the seeds are fully exposed to concentrated sulfuric acid, pour out the concentrated sulfuric acid and rinse the seeds with tap water for more than 3 times to ensure that there is no concentrated sulfuric acid After 10-15 minutes, when the temperature drops to 30°C, stop stirring and transfer the seeds to 25% carbendazim wettable powder In 250 times liquid, continue to soak for 5-10 minutes, take out the seeds and wash them with distilled water, transfer them to a petri dish, add distilled water, the distilled water should just submerge the seeds, cover the lid of the petri dish, and put it in the light incubator Cultivate under light until the seeds are white, and count the germination rate;
5)种子播种:将育苗基质装填在漂浮育苗盘中,然后用配套的播种器在育苗盘上压出1.5cm深的小孔,再用镊子将发芽的种子夹到孔中,使根朝下,上方均匀撒上一层育苗基质,抹平,最后放入育苗池中培养;5) Seed sowing: Fill the seedling raising substrate in the floating seedling raising tray, then use the matching seeder to press out a 1.5cm deep hole on the seedling raising tray, and then use tweezers to clamp the germinated seeds into the hole so that the root faces downward , evenly sprinkle a layer of seedling substrate on top, smooth it, and finally put it into the seedling pond for cultivation;
6)日常管理:在生长期间,观察育苗池中水的深浅变化,不定时向池中加水保证池子中有充足的水,15天加一次;齐苗后3-5天,按15g/盘的量施入育苗肥,每隔7天施一次肥,播种30-40天后,待苗长到8-10cm,进行移栽。6) Daily management: During the growth period, observe the change of water depth in the nursery pool, add water to the pool from time to time to ensure that there is sufficient water in the pool, add once every 15 days; Apply a large amount of seedling fertilizer, and apply fertilizer every 7 days. After 30-40 days of sowing, wait for the seedlings to grow to 8-10cm before transplanting.
实施例2Example 2
一种甘薯实生种子漂浮育苗方法,具体的步骤为:A method for floating seedling raising of sweet potato seeds, the specific steps are:
1)建育苗池:用小红砖搭建池子,在池子底部铺上一层塑料膜,并且池子上方活动罩设有一个用钢架焊接而成的拱棚,拱棚外围用厚塑料薄膜封好,拱棚两端设置有可自由掀开的厚塑料薄膜,待苗池建好后,往池子中注入自来水,水位不漫过池子;其中,池子的大小为长×宽×高=330cm×70cm×10cm,拱棚的规格为长×宽×高=370cm×90cm×80cm;1) Build a nursery pool: build a pool with small red bricks, spread a layer of plastic film on the bottom of the pool, and set a shed welded with a steel frame on the movable cover above the pool. Both ends are provided with thick plastic films that can be opened freely. After the seedling pond is built, tap water is poured into the pond, and the water level does not overflow the pond; the size of the pond is length×width×height=330cm×70cm×10cm, The specification of the shed is length × width × height = 370cm × 90cm × 80cm;
2)漂浮育苗盘准备:选用规格为长×宽×高=66cm×33.5cm×5.5cm的200孔聚苯乙烯泡沫塑料盘作为漂浮育苗盘,并选择配套的播种器(如图6、图7所示,能一次性完成打孔过程,节省人力);2) Floating seedling tray preparation: select the 200-hole polystyrene foam tray as the floating seedling tray for length * width * height = 66cm * 33.5cm * 5.5cm, and select supporting seeders (as shown in Figure 6 and Figure 7 As shown, the punching process can be completed at one time, saving manpower);
3)育苗基质:沼渣(作物秸秆、牛粪、猪粪等经沼气池发酵后所得,主要成分为有机质和腐殖质等)晒干、碾碎、过20目筛,备用;肥沃的园土,去除杂物,晒干,碾碎,过20目筛,备用;按沼渣:蛭石:园土:草木灰=6:2:20:1的重量比配制育苗基质,将上述材料按比例充分混匀后,用高压灭菌锅121℃灭菌40分钟,充分杀灭病菌、虫卵、杂草种子等,使用前先加水拌湿,基质含水量为60%,即手捏成团,放下呈散开的状态;3) Seedling-raising substrate: biogas residue (crop straw, cow dung, pig manure, etc. obtained after fermentation in a biogas tank, the main components are organic matter and humus, etc.) dried in the sun, crushed, and passed through a 20-mesh sieve for use; fertile garden soil, Remove sundries, dry, crush, cross 20 mesh sieves, and set aside; prepare seedling-raising substrate according to the weight ratio of biogas residue: vermiculite: garden soil: plant ash=6:2:20:1, and the above-mentioned materials are fully mixed in proportion. After evenly mixing, sterilize in an autoclave at 121°C for 40 minutes to fully kill germs, insect eggs, weed seeds, etc., add water and mix before use, the water content of the matrix is 60%, that is, hand knead into a ball, put it down into a ball scattered state;
4)实生种子播种前处理:将甘薯实生种子用98%浓硫酸浸泡30分钟,且不断搅拌,使种子充分接触浓硫酸,将浓硫酸倒出并用自来水将种子冲洗3遍以上,保证无浓硫酸残留,然后转入50-55℃温水中浸泡,边浸泡边搅拌,使种子受热均匀,10-15分钟后温度降至30℃时,停止搅拌,将种子转入25%多菌灵可湿性粉剂250倍液中,继续浸泡5-10分钟,取出种子用蒸馏水洗净,转入培养皿中,加入蒸馏水,蒸馏水以刚好浸没种子为宜,将培养皿的盖子盖好,放入25℃光照培养箱中光照培养48h,每24h换一次水,培养至种子露白,统计发芽率;4) Pre-sowing treatment of seeds: Soak the seeds of sweet potato with 98% concentrated sulfuric acid for 30 minutes, and keep stirring, so that the seeds are fully exposed to concentrated sulfuric acid, pour out the concentrated sulfuric acid and rinse the seeds with tap water for more than 3 times to ensure that there is no concentrated sulfuric acid After 10-15 minutes, when the temperature drops to 30°C, stop stirring and transfer the seeds to 25% carbendazim wettable powder In 250 times liquid, continue to soak for 5-10 minutes, take out the seeds and wash them with distilled water, transfer them to a petri dish, add distilled water, the distilled water should just submerge the seeds, cover the lid of the petri dish, and put it in 25℃ for light cultivation Cultivate under light in the box for 48 hours, change the water every 24 hours, cultivate until the seeds are white, and count the germination rate;
5)种子播种:将育苗基质装填在漂浮育苗盘中,然后用配套的播种器在育苗盘上压出1.5cm深的小孔,再用镊子将发芽的种子夹到孔中,使根朝下,上方均匀撒上一层育苗基质,抹平,最后放入育苗池中培养;5) Seed sowing: Fill the seedling raising substrate in the floating seedling raising tray, then use the matching seeder to press out a 1.5cm deep hole on the seedling raising tray, and then use tweezers to clamp the germinated seeds into the hole so that the root faces downward , evenly sprinkle a layer of seedling substrate on top, smooth it, and finally put it into the seedling pond for cultivation;
6)日常管理:在生长期间,观察育苗池中水的深浅变化,不定时向池中加水保证池子中有充足的水,15天加一次;齐苗后3-5天,按15g/盘的量施入育苗肥,育苗肥为N:P2O5:K2O=20:7:13的复合肥,氮浓度控制在300mg/L,每隔7天施一次肥,播种30-40天后,待苗长到8-10cm,进行移栽。6) Daily management: During the growth period, observe the depth of water in the nursery pond, add water to the pond from time to time to ensure that there is sufficient water in the pond, and add once every 15 days; Apply the seedling fertilizer in a large amount, the seedling fertilizer is the compound fertilizer of N:P 2 O 5 :K 2 O=20:7:13, the nitrogen concentration is controlled at 300mg/L, and the fertilizer is applied every 7 days, after 30-40 days of sowing , when the seedlings grow to 8-10cm, transplant.
实验分析experiment analysis
2015年9月3号进行漂浮育苗和常规育苗试验,具体实施方案如下:On September 3, 2015, floating seedling raising and conventional seedling raising tests were carried out. The specific implementation plan is as follows:
1)2015年9月3号,进行甘薯实生种子播前催芽处理,选取杂交组合1510号的实生种子450粒,装入烧杯中,用98%浓硫酸浸泡30分钟,且不断搅拌,使种子充分接触浓硫酸,尤其是前10分钟,需充分搅拌,使所有种子与浓硫酸充分接触,腐蚀种子表面坚硬的角质层,将浓硫酸倒出并用自来水将种子冲洗3遍以上,保证无浓硫酸残留;然后转入55-60℃温水中浸泡,边浸泡边搅拌,使种子受热均匀,10-15分钟后温度降至30℃时,停止搅拌,将种子转入25%多菌灵可湿性粉剂250倍液中,继续浸泡5-10分钟,取出种子用蒸馏水洗净,转入培养皿中(150粒为一个重复,设3次重复),加入蒸馏水,蒸馏水以刚好浸没种子为宜,将培养皿的盖子盖好,放入25℃光照培养箱中光照培养,12小时换一次水,24-48小时后种子露白即可播种,间隔24h和48h分别统计发芽率。1) On September 3, 2015, the germination treatment of sweet potato seeds before sowing was carried out, and 450 seeds of the hybrid combination No. 1510 were selected, put into a beaker, soaked in 98% concentrated sulfuric acid for 30 minutes, and kept stirring to make the seeds fully When exposed to concentrated sulfuric acid, especially in the first 10 minutes, it needs to be fully stirred so that all the seeds are in full contact with concentrated sulfuric acid to corrode the hard cuticle on the surface of the seeds. Pour out the concentrated sulfuric acid and rinse the seeds with tap water for more than 3 times to ensure that there is no concentrated sulfuric acid residue then soak in warm water at 55-60°C, stir while soaking, so that the seeds are heated evenly, and when the temperature drops to 30°C after 10-15 minutes, stop stirring, and transfer the seeds to 25% carbendazim WP 250 In the double liquid, continue to soak for 5-10 minutes, take out the seeds and wash them with distilled water, transfer them to a petri dish (150 grains are one repetition, set 3 repetitions), add distilled water, it is advisable to just immerse the seeds in the distilled water, put the petri dish Cover the lid, put it into a light incubator at 25°C for light cultivation, change the water every 12 hours, and sow the seeds after 24-48 hours, and count the germination rate at intervals of 24h and 48h.
2)2015年9月6号,选择干净的漂浮盘填入拌湿的干净育苗基质,并用配套播种器在育苗盘上压出1.5cm深的小孔,挑选已经发芽的种子种入育苗盘孔穴中央,盖上基质土,抹平,放入注好水的育苗池中培养,设3个重复,每个重复选择50粒发芽种子,将拱棚两端的薄膜放下,并用砖压好,保证不留缝隙。2) On September 6, 2015, choose a clean floating tray and fill it with a clean seedling medium mixed with water, and press out a 1.5cm deep hole on the seedling tray with a matching seeder, and select germinated seeds and plant them into the hole of the seedling tray In the center, cover with matrix soil, smooth it, put it into the water-filled seedling pond for cultivation, set up 3 repetitions, select 50 germinated seeds for each repetition, put down the film at both ends of the shed, and press it with bricks to ensure that no seeds remain. gap.
3)2015年9月6号,同一天进行苗床育苗,先建苗床,苗床大小为长100cm,宽80cm,高15cm,把地块先深翻整平,不留土块,然后在地块上面铺一层5cm高的育苗基质,浇透水,把苗床均匀分成3小块,先用镊子将苗床划出一条深2cm的小沟,然后将发芽的种子均匀排列在沟中,然后盖上基质土,抹平,再浇一次水,设3个重复,每重复选择50粒发芽种子,播种完后用细竹条弯成拱形插到苗床两侧,然后铺上薄膜,建成小拱棚,7天浇一次水,保证苗床湿润。3) On September 6, 2015, the seedbed was raised on the same day. The seedbed was built first. The size of the seedbed was 100cm long, 80cm wide, and 15cm high. Spread a layer of 5cm-high seedling substrate, water thoroughly, divide the seedbed into 3 small pieces evenly, first use tweezers to draw a small ditch 2cm deep in the seedbed, then arrange the germinated seeds evenly in the ditch, and then cover with substrate soil , smooth, water again, set 3 repetitions, select 50 germinated seeds for each repetition, bend thin bamboo strips into arches and insert them on both sides of the seedbed after sowing, and then spread film to build a small arch shed, 7 days Water once to keep the seedbed moist.
4)播种后每天观察并统计出苗数,作好记录,齐苗后5天,即2015年9月15号,第一次统一施育苗肥,育苗池按15g/盘的量称150g复合肥(N:P2O5:K2O=20:7:13)均匀撒施到育苗池中,并搅拌均匀,灌满水;苗床称15g复合肥溶解到1000ml水中,均匀浇灌到实生苗上,间隔7天统一按上述方法施一次肥料。4) After sowing, observe and count the number of seedlings every day, and make a record. After 5 days after the seedlings are assembled, that is, on September 15, 2015, the seedling fertilizer is uniformly applied for the first time, and the seedling pond is called 150g compound fertilizer by the amount of 15g/pan ( N:P 2 O 5 :K 2 O=20:7:13) evenly sprinkled in the nursery pool, and stirred evenly, filled with water; the seedbed said that 15g of compound fertilizer was dissolved in 1000ml of water, and evenly poured on the seedlings, Apply fertilizer uniformly according to the above method every 7 days.
5)播种后30天,即2015年10月6号,每处理重复取样10株,测量根长、株高、根数、最大叶的叶长和叶宽、根鲜重、茎叶鲜重并计算根冠比=根鲜重/茎叶鲜重、根鲜重比=根鲜重/整株鲜重×100、叶长宽乘积=叶长×叶宽。5) 30 days after sowing, that is, on October 6, 2015, 10 plants were repeatedly sampled for each treatment, and the root length, plant height, root number, leaf length and leaf width of the largest leaf, root fresh weight, and stem and leaf fresh weight were measured. Calculate root-shoot ratio = root fresh weight/stem-leaf fresh weight, root fresh weight ratio=root fresh weight/whole plant fresh weight×100, leaf length-width product=leaf length×leaf width.
6)结果与分析6) Results and analysis
浓硫酸处理甘薯实生种子的发芽率情况统计见表1。See Table 1 for statistics on the germination rate of sweet potato seeds treated with concentrated sulfuric acid.
表1浓硫酸处理甘薯实生种子的发芽率Table 1 Germination rate of sweet potato seeds treated with concentrated sulfuric acid
从表1中可看出,经过浓硫酸处理后的甘薯实生种子的平均发芽率达到92.55%。如不是种子本身的问题,严格按照预处理操作要求操作,发芽率可以达到100%。As can be seen from Table 1, the average germination rate of sweet potato seeds after concentrated sulfuric acid treatment reaches 92.55%. If it is not the problem of the seeds themselves, the germination rate can reach 100% if the pretreatment operation requirements are strictly followed.
漂浮育苗和苗床育苗的甘薯实生种子出苗率见表2。See Table 2 for the emergence rate of sweet potato seedlings raised in floating seedlings and seedbeds.
表2不同育苗方式甘薯实生种子出苗率Table 2 The emergence rate of sweet potato seeds in different seedling raising methods
从表2中可以看出,漂浮育苗和苗床育苗两种方式对甘薯实生种子的出苗速度和出苗率没有显著差异,3-4天均能够齐苗,基本可以达到100%的出苗率。As can be seen from Table 2, there is no significant difference in the emergence speed and emergence rate of sweet potato seeds in two ways of floating seedling cultivation and seedbed cultivation, and the seedlings can be assembled in 3-4 days, and the emergence rate of 100% can be basically achieved.
漂浮育苗和苗床育苗的甘薯实生苗的生物学性状见表3。See Table 3 for the biological characteristics of sweet potato seedlings raised in floating seedlings and seedbeds.
表3不同育苗方式甘薯实生苗的生物学性状Table 3 Biological characters of sweet potato seedlings in different seedling raising methods
从表3中可以看出,漂浮育苗方式的实生苗株高小于苗床育苗,并且差异显著,说明苗床育苗生长快,但从实际观察看属于徒长,容易倒伏。但漂浮育苗的根长、根数、叶长宽乘积分别比苗床育苗增加了26.18%、39.94%、33.43%,根数差异极显著,叶长宽乘积差异显著,说明漂浮育苗根系比较发达,叶片面积比较大,为吸收更多的养分和制造更多的光合产物打下坚实的基础。It can be seen from Table 3 that the plant height of the seedlings raised by floating seedlings is smaller than that of seedlings raised in seedbeds, and the difference is significant, indicating that seedlings grown in seedbeds grow fast, but from actual observations, they are leggy and easy to lodging. However, the root length, root number, and leaf length-width product of floating seedlings increased by 26.18%, 39.94%, and 33.43% respectively compared with seedbed cultivation. Lay a solid foundation for absorbing more nutrients and producing more photosynthetic products.
漂浮育苗和苗床育苗的甘薯实生苗的生物量见表4。See Table 4 for the biomass of sweet potato seedlings raised in floating seedlings and seedbeds.
表4不同育苗方式甘薯实生苗的生物量Table 4 Biomass of sweet potato seedlings in different seedling raising methods
从表4中可以看出,漂浮育苗方式的甘薯实生苗根鲜重、根鲜重比、根冠比均极显著高于苗床育苗,分别比后者高73.01%、77.02%、76.47%,说明漂浮育苗更有利于根的生长,增加根冠比,为作物创造良好的生长条件。而苗床育苗的根系明显不发达,茎叶生长太旺,容易倒伏,不利于甘薯苗的移栽。It can be seen from Table 4 that the root fresh weight, root fresh weight ratio, and root-to-shoot ratio of sweet potato seedlings raised in floating seedlings were significantly higher than those raised in seedbeds, which were 73.01%, 77.02%, and 76.47% higher than the latter, indicating that Floating seedlings are more conducive to root growth, increase the ratio of root to shoot, and create good growth conditions for crops. However, the root system of seedbed cultivation is obviously underdeveloped, and the stems and leaves grow too vigorously, which is easy to lodging, which is not conducive to the transplanting of sweet potato seedlings.
附图1-5均为育苗试验时的实拍照片,如图1所示,苗床育苗方法的苗床上杂草较多,实生苗生长不整齐,基部出现弯曲现象,叶子有被害虫啃噬现象;如图2所示,漂浮育苗方法的育苗盘上没有杂草生长,实生苗生长整齐一致,无病虫害,也没有倒伏现象发生;如图3所示,苗床育苗方法的实生苗移栽时,根部基本不带土,严重影响移栽成活率,而且基部、中部均出现弯曲现象,说明苗床育苗方法育出的实生苗易出现倒伏现象;如图4所示,漂浮育苗生长一致,根系发达,而且从育苗盘取出后可以将孔穴中的基质土全部取出,大大增加移栽成活率,而且无弯曲倒伏情况;如图5所示,漂浮育苗和苗床育苗方法相比较,漂浮育苗法的实生苗生长整齐一致,无弯曲倒伏,根系发达、密集。Accompanying drawings 1-5 are the real photos taken during the seedling raising test, as shown in Figure 1, there are more weeds on the seedbed of the seedbed raising method, the seedlings grow irregularly, the base appears bent, and the leaves are bitten by pests ; As shown in Figure 2, there is no weed growth on the seedling tray of the floating seedling raising method, and the seedling growth is neat and consistent, without damage by disease and insect, and there is no lodging phenomenon; As shown in Figure 3, when the seedling of the seedbed raising method is transplanted, The roots are basically without soil, which has a strong impact on the survival rate of transplanting, and there are bending phenomena in the base and the middle, which shows that the seedlings raised by the seedbed method are prone to lodging; as shown in Figure 4, the growth of floating seedlings is consistent, and the roots are developed And after taking out from the seedling tray, the matrix soil in the hole can be taken out completely, greatly increasing the transplanting survival rate, and there is no bending and lodging situation; The growth is neat and consistent, without bending and lodging, and the root system is developed and dense.
传统苗床育苗,育出的实生苗容易徒长,需要人员随时照看、揭膜炼苗,而且易发生病虫害,需要喷洒农药进行防治,增加育种成本;而本发明因为育苗拱棚较高,可供实生苗生长的空间较大,不会出现徒长。传统苗床育苗,正如本试验进行的一样,根系很弱,移栽时基本带不上土,故移栽成活率不高,移栽前期需要频繁浇水,增加成本,而本发明移栽成活率较高,浇水次数少。Seedlings grown in traditional seedbeds tend to grow excessively, requiring personnel to take care of them at any time, remove the film to harden seedlings, and are prone to disease and insect pests, which need to be sprayed with pesticides for prevention and control, which increases the cost of breeding; however, the present invention can be used for seedlings because of the high arched shed for seedlings. The space for growth is larger, and there will be no leggy growth. Traditional seedbed cultivation, just as this test is carried out, the root system is very weak, and the soil cannot be brought up when transplanting, so the survival rate of transplanting is not high, and frequent watering is required in the early stage of transplanting, which increases costs, while the survival rate of transplanting in the present invention Higher and less frequent watering.
本发明育出的实生苗,可先不直接移栽到大田,而是先移栽到温室大棚进行二次培育,在温室大棚中进行株行距为10cm×10cm的密度栽培实生苗,生长2个月后实生苗可长至80cm-100cm,此时再剪苗移栽至大田,可增加优良株系的育种入选率,并能一次得到较多甘薯块根,第二年实现快速繁苗,缩短育种年限;而且也可以实现同一实生苗多地点选择,提高育种效率,缩短新品种选育进程。The seedlings bred by the present invention can not be directly transplanted to the field earlier, but are first transplanted to the greenhouse for secondary cultivation, and the density cultivation of the seedlings with a row spacing of 10cm×10cm is carried out in the greenhouse, and 2 seedlings are grown. After one month, the seedlings can grow to 80cm-100cm. At this time, the seedlings can be cut and transplanted to the field, which can increase the breeding selection rate of excellent lines, and can obtain more sweet potato tubers at one time. In the second year, rapid seedling propagation can be achieved, shortening the breeding time. The number of years; and it can also realize the multi-site selection of the same seedling, improve the breeding efficiency, and shorten the process of new variety selection.
本发明可进行多次筛选:第一次筛选:漂浮育苗移栽至温室大棚时,可根据实生苗强弱,淘汰一部分弱苗;第二次筛选:温室大棚培育壮苗移栽至大田时,又可淘汰一部分弱苗;第三次筛选:大田收获时,根据育种要求选择表现好的株系收获,并且能实现同一实生苗一年多地同时筛选,提高育种效率,缩短新品种选育进程。The present invention can perform multiple screenings: the first screening: when the floating seedlings are transplanted to the greenhouse, some weak seedlings can be eliminated according to the strength of the seedlings; the second screening: when the strong seedlings are cultivated in the greenhouse and transplanted to the field, Part of the weak seedlings can also be eliminated; the third screening: When harvesting in the field, select the strains with good performance according to the breeding requirements for harvesting, and can realize the simultaneous screening of the same seedlings for more than one year, improve the breeding efficiency, and shorten the process of new variety selection .
甘薯实生苗在漂浮池中根系生长非常旺盛,这有利于其吸收养分,促进实生苗快速生长,但不利于后期移栽工作,故在生长后期炼苗阶段需要把生长到漂浮池中的多余根系剪断,与育苗盘底部齐平即可,剪根后在继续培养7天,即可移栽。剪根后炼苗效果好,移栽时能够轻松的将实生苗和育苗盘孔穴中的基质土全部取出,而不会出现因底部根系太长引起的基质土松散的情况。The root system of sweet potato seedlings grows very vigorously in the floating pond, which is conducive to their absorption of nutrients and promotes the rapid growth of seedlings, but it is not conducive to the later transplanting work. Cut off and be flush with the bottom of the seedling tray. After cutting the roots, continue to cultivate for 7 days before transplanting. After cutting the roots, the seedling hardening effect is good. When transplanting, all the substrate soil in the seedlings and the seedling tray holes can be easily taken out without loosening the substrate soil caused by the root system at the bottom being too long.
漂浮育苗能够在一个育苗盘上容纳200粒或更多种子,一个育苗池就可容纳2000粒甚至更多种子,所占空间小,所用育苗基质少,一次性育苗数量多,育苗时间短,但是如果需要剪苗移栽则需要移栽到温室大棚进行二次培育。Floating seedlings can accommodate 200 or more seeds on a seedling tray, and a seedling pool can accommodate 2,000 or more seeds, occupying a small space, using less seedling substrates, a large number of seedlings at one time, and short seedling time, but If the seedlings need to be cut and transplanted, they need to be transplanted to the greenhouse for secondary cultivation.
最后说明的是,以上优选实施例及附图仅用以说明本发明的技术方案而非限制,尽管通过上述优选实施例已经对本发明进行了详细的描述,但本领域技术人员应当理解,可以在形式上和细节上对其作出各种各样的改变,而不偏离本发明权利要求书所限定的范围。Finally, it should be noted that the above preferred embodiments and accompanying drawings are only used to illustrate the technical solutions of the present invention rather than limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that Various changes may be made in form and detail without departing from the scope of the invention defined by the claims.
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