CN105768106A - Liver nutrition improving functional food - Google Patents
Liver nutrition improving functional food Download PDFInfo
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- CN105768106A CN105768106A CN201610169377.9A CN201610169377A CN105768106A CN 105768106 A CN105768106 A CN 105768106A CN 201610169377 A CN201610169377 A CN 201610169377A CN 105768106 A CN105768106 A CN 105768106A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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Abstract
The present invention belongs to the field of health-care food and particularly relates to a liver nutrition improving functional food. The food consists of the following raw material compositions: 10-80 parts of whey protein concentrate, 5-50 parts of corn oligopeptides, 0.2-3 parts of DHA algae oil powder, 1-10 parts of taurine, 0.2-5 parts of grape seed extract, and 10-80 parts of maltodextrin. The provided liver nutrition improving functional food can relatively better improve the health condition of alcoholic liver injury, improves human immunity, and has a significant effect especially in terms of lowering blood lipids.
Description
Technical field:
The invention belongs to field of health care food, be specifically related to a kind of functional food contributing to liver nutrition improvement.
Background technology:
Liver is the organ that body weight for humans is wanted, and is also simultaneously the important immune organ of body.Various virulence factor effects
After hepatic tissue, liver cell infringement in various degree can be directly resulted in.Alcoholic liver injury is due to long-term a large amount of
Ethanol intake and cause with hepatic steatosis, the one that inflammatory cell infiltration, even fibrillatable are characterized
Poisoning liver diseases.After body repeated multiple times Excess free enthalpy ethanol, ethanol can by induction CYP4502E1,
Cause oxidative stress, lipid peroxidation, the disorder of metabolism/nutrition and structure of mitochondria and the change etc. of function
Series reaction, causes hepatocellular function damage, and then causes content of triglyceride in hepatic tissue to build up, courage
Sterol synthesis is strengthened, and causes the change of corresponding apolipoprotein content.
In China recently as the notable growth of alcohol consumption per capita, the incidence of disease of alcoholic liver injury also in by
Year ascendant trend, increasing people is to protecting the liver, protect liver, preventing the treatment of liver fibrosis self regulation this from having
Demand, also has suitable enterprises and individuals that this has made much work at present both at home and abroad.
The Chinese invention patent of Application No. 200510064227.3 discloses a kind of health care with liver boosting function
Food, the raw material of preparing of the active component that it is contained comprises: ant 1 part, fruit of Chinese magnoliavine 0.1-10 part, after testing
The every 100g of this invention contains: acid adding 2.52mh, schizandrin A 1.9mg, deoxyschizandrin 2.2mg.This invention
There is the function having auxiliary protection function to chemical damage, be suitable for there is chemical damage danger person.
Chinese patent CN102599503B discloses a kind of health food having more facilitating alcohol metabolism and protecting liver effect and system thereof
Preparation Method, the raw material of this health food includes following components and weight percent content: aminobutyric acid 20-25%,
Theanine 15-20%, zein hydrolysate 15-20%, grape seed extract 10-15%, Milk Thistle grass extracts
Thing 10-15%, kudzu root extract 10-15%, compared with the prior art, it is excellent that this invention has facilitating alcohol metabolism and protecting liver function etc.
Point.
The Chinese invention patent of Application No. 201110008375.9 discloses one to be improved liver function and reduces liver
The composition of damage, comprises fruit of Chinese magnoliavine compound powder, Cobastab group, glycine, a species of small clam living in fresh water meat extract, grape
Saccharic acid zinc, globe artichoke and Cordyceps sinensis, this invention can promote hepatocellular fat metabolism, prevention fatty liver and liver
Hardening, also repairs liver cell simultaneously, helps liver detoxification, reduces hepatic injury.
Maize oligopeptide is with corn protein powder as raw material, through enzymolysis, separate, refined and the process such as be dried
And the relative molecular weight prepared is at the little molecule oligopeptide of 200～800Dalton.The amino acid group of maize oligopeptide
Become quite similar, rich in branched-chain amino acid (leucine, isoleucine, figured silk fabrics with the amino acid of zein composition
Propylhomoserin), glutamic acid, alanine, proline.The Amino acid profile that maize oligopeptide is unique, may advantageously facilitate
In liver, the metabolism of alcohol, plays a protective role to liver
The present invention will provide a kind of health products carrying out liver nutrition improvement for alcoholic liver injury.
Summary of the invention:
A kind of functional food contributing to liver nutrition improvement, is made up of the raw material of following parts by weight: concentrate breast
Albumin 10-80 part, maize oligopeptide 5-50 part, DHA algal oil-bound distemper 0.2-3 part, taurine 1-10 part,
Grape seed extract 0.2-5 part, maltodextrin 10-80 part；
The preparation method of described maize oligopeptide is as follows:
(1) in corn protein powder, add the water of w/v 10-20 times, mix, adjust pH8-10,
It is heated to 50-80 DEG C of insulated and stirred 20-60min；Feed liquid is discarded clear liquid after centrifugation operates, stays
Use slag charge；Repeat the above steps obtains slag charge 1-3 time；
(2) water (m:v) adding 8-15 times in above-mentioned slag charge is sized mixing, regulation pH8-10, temperature 50-70 DEG C,
Alkali protease, enzymolysis 2-3h is added by 8000-10000U/g corn protein powder；
(3) after being cooled to 30-50 DEG C, pH5-7 is regulated with streptococcus acidi lactici fermented solution, by 10000-15000U/g corn
Albumen powder adds neutral proteinase, enzymolysis 5-6h；
(4) regulation pH6-7, temperature 40-60 DEG C, add flavor protease by 400-1000U/g corn protein powder,
Enzymolysis 3-5h, after enzymolysis terminates, is heated to 85 DEG C-95 DEG C, and go out enzyme 15-20 minute；
(5), after centrifugal concentrating, it is spray-dried；
The preparation method of described streptococcus acidi lactici fermented solution is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access equipped with 50mL culture medium
In the 250mL triangular flask of MRS (without agar, concentration of glucose is 150g/L) culture medium, 200rpm, 37 DEG C
Cultivate 12-16h, make thalline be in mid log phase；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is at 10-15%, 35-38 DEG C
100-200rpm cultivates 8-12 hour, and dissolved oxygen controls 10% (ventilation 0.5L/min), Anaerobic culturel the most again
60-70 hour；
Described fermentation medium composition is as follows: peptone 10-12g, beef extract 10-12g, yeast extract 5-8g,
Diammonium hydrogen citrate 2-4g, glucose 20-25g, Tween 80 1-2mL, sodium acetate 5-7g, phosphoric acid hydrogen two
Potassium 2-3g, magnesium sulfate 0.58-0.6g, manganese sulfate 0.25-0.3g, cholesterol 100-120mg, Chinese herbal medicine powder
5-8g, distilled water 1 000mL, pH 6.2～6.6；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is
The cholesterol solution of 10-12mg/mL, adds in culture medium the most by a certain percentage, makes cholesterol ultimate density
For 100-120 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh fruit of Chinese magnoliavine 10-20 part；Radix Codonopsis 10-15 part；Hawthorn 10-20 part；Respectively by said herbal medicine powder
Being broken to particle diameter is less than 2 millimeters, then uniformly mixes and add the water of 3-6 times of weight in container, controls temperature
Spending 45-60 DEG C and keep 2～4h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6-7, enzymolysis 2-4h,
Finally add 0.5-3 times of w ethanol of mixed material and the mixture of propyl alcohol, ultrasonic extraction under 110W power
0.5～1.5h, filter；Filter vacuum concentrates postlyophilization and obtains Chinese herbal medicine powder；
Described mixed enzyme addition is the 5-10% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 15-20 part, beta amylase 10-15 part, pectin
Enzyme 10-15 part, acid protease 10-15 part, acid phosphatase 5-10 part；
The mass ratio of described ethanol and propyl alcohol is 1:1-2.
The preparation method of described grape seed extract is as follows:
(1) grape pip is pulverized, add water and the lactic acid bacteria of 0.05%-0.15% of grape pip weight 20%-30%
Bacterium powder, room temperature lower seal ferments 3-5 days, is dried under vacuum to moisture content≤5%, obtains pre-processing grape pip powder；
(2) being placed in pretreatment grape pip powder equipped with in 0.3-0.5% sodium bicarbonate solution, regulation pH value is 7-8,
It is warming up to 40-50 DEG C, adds the mixed enzyme of pretreatment grape pip opaque amount 2-8%, stir, enzymolysis
40-70min, carries out high-pressure pulse electric process, electric-field intensity 20-40kV/cm, burst length during enzymolysis
400-500 μ s, pulse frequency 200-300Hz；The enzymolysis liquid of above-mentioned process filters through 100-300 eye mesh screen, filtrate
Freeze-dried i.e. obtain grape seed extract；The quality group of described mixed enzyme becomes: amylase: pectase: fiber
Element enzyme=1:5-8:5-10；
Described lactic acid bacteria is specially Lactobacillus plantarum (Lactobacillus plantarum) XH, and this bacterium is in 2015
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 30, in (to be called for short
CGMCC), preserving number is CGMCC NO.11763, and preservation address is: city of the BeiJing, China Chaoyang District North Star
West Road 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101；
Remaining raw material is commercially available prod；
The preparation method of the described functional food contributing to liver nutrition improvement is: process by this area conventional method
For tablet, pulvis, granule, soft capsule, hard shell capsules, oral liquid etc., directly take, it is also possible to as food
Product raw material uses.Every consumption per day is 5～10g.
Beneficial effect:
1, the functional food contributing to liver nutrition improvement provided by the present invention can preferably improve human body and exempts from
Epidemic disease power, particularly in terms of reducing blood lipid, effect is notable.
2, product provided by the present invention have employed Lactobacillus plantarum CGMCC in preparation process first
The method of NO.11763 zymotic fluid acid adjustment, significantly improves the Lowering cholesterol effect of product.CGMCC
NO.11763 is having the property that 1. degrading nitrite speed under fermentation culture conditions of the present invention
Degree is fast, and capacity of decomposition reaches 10.9mg/kg h, can effectively decompose people due to improper diet and take in
Nitrite；2. this bacterium is high to degrading rate of cholesterol, can reach 64.76%, be particularly suited for obese people and
Three high patients；3. this bacterium Adhering capacity is high, and mensuration is 95.71% from aggegation rate, can effectively be colonizated in stomach
In intestinal tract, give full play to its physiological activity.Although at present to improving product fall courage during its acid adjustment
The mechanism of sterol effect is unclear, but also for health products exploitation with preparation provide new approach.
3, product provided by the present invention have employed lactobacillus-fermented and locates in advance during grape seed extract extracts
The method of reason, the method can be effectively improved produces the effect improving immunity of organisms, and method the most easily operates.
Detailed description of the invention
1 one kinds of functional foods contributing to liver nutrition improvement of embodiment and preparation method thereof
It is made up of the raw material of following parts by weight: WPC 10 parts, maize oligopeptide 5 parts, DHA
Algae oil-bound distemper 0.2 part, taurine 1 part, grape seed extract 0.2 part, maltodextrin 80 parts；
Weigh each component according to above-mentioned formula rate, pulverize, cross 80 mesh sieves, then mix 10min, obtain powder
Agent.
The preparation method of described maize oligopeptide is as follows:
(1) in corn protein powder, add the water of w/v 10 times, mix, adjust pH8, heating
To 50 DEG C of insulated and stirred 20min；Feed liquid is discarded clear liquid after 5000rpm centrifugation operates, continues to employ
Slag charge；Repeat the above steps obtains slag charge 1 time；
(2) water (m:v) adding 8 times in above-mentioned slag charge is sized mixing, and regulates pH8, and temperature 50 C, by 8000U/g
Corn protein powder adds alkali protease, enzymolysis 2h；
(3) regulate pH5, temperature 30 DEG C with streptococcus acidi lactici fermented solution, add neutrality by 10000U/g corn protein powder
Protease, enzymolysis 5h；
(4) regulation pH6, temperature 40 DEG C, add flavor protease, enzymolysis 3h by 400U/g corn protein powder；,
After enzymolysis terminates, being heated to 85 DEG C, go out enzyme 15 minutes；
(5), after 4000r/min is centrifuged reduced pressure concentration, maize oligopeptide it is spray-dried to obtain；
The preparation method of described streptococcus acidi lactici fermented solution is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access equipped with 50mL culture medium
In the 250mL triangular flask of MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 12h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 10%, and at 35 DEG C, 100rpm cultivates 8
Hour, dissolved oxygen controls 10% (ventilation 0.5L/min), Anaerobic culturel 60 hours afterwards；
Described fermentation medium composition is as follows: peptone 10g, beef extract 10g, yeast extract 5g, lemon
Lemon acid hydrogen two ammonium 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, sulfuric acid
Magnesium 0.58g, manganese sulfate 0.25g, cholesterol 100mg, Chinese herbal medicine powder 5g, distilled water 1 000mL, pH
6.2；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is 10mg/mL
Cholesterol solution, add to the most by a certain percentage in culture medium, making cholesterol ultimate density is 100 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh 10 parts of the fruit of Chinese magnoliavine；Radix Codonopsis 10 parts；Hawthorn 10 parts；Respectively said herbal medicine is crushed to particle diameter
It is less than 2 millimeters, in container, then uniformly mixes and add the water of 3 times of weight, control temperature 45 C and protect
Holding 2h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6, and enzymolysis 2h finally adds mixed material
0.5 times of w ethanol and the mixture of propyl alcohol, ultrasonic extraction 0.5h under 110W power, filters；Filter vacuum
Concentrate postlyophilization and obtain Chinese herbal medicine powder；
Described mixed enzyme addition is the 5% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 15 parts, beta amylase 10 parts, pectase 10 parts,
Acid protease 10 parts, acid phosphatase 5 parts；
The mass ratio of described ethanol and propyl alcohol is 1:1.
The preparation method of described grape seed extract is as follows:
(1) grape pip was pulverized 80 mesh sieves, added the water and 0.05% of grape pip weight 20% (v:m)
Lactic acid bacteria bacterium powder, room temperature lower seal ferments 3-5 days, is dried under vacuum to moisture content≤5%, obtains pre-processing Portugal
Grape seed powder；
(2) pretreatment grape pip powder is placed in 0.3% sodium bicarbonate solution equipped with 5 times of volumes (m:v), adjusts
Joint pH value is 7, is warming up to 40 DEG C, adds the mixed enzyme of pretreatment grape pip opaque amount 2%, stirs,
Enzymolysis 40min, carries out high-pressure pulse electric process during enzymolysis, electric-field intensity 20kV/cm, burst length 400 μ s,
Pulse frequency 200Hz；The enzymolysis liquid of above-mentioned process filters through 100 eye mesh screens, filtrate freeze-dried Ji get Portugal
Grape seed extract；The quality group of described mixed enzyme becomes: amylase: pectase: cellulase=1:5:5；
Described lactic acid bacteria is specially Lactobacillus plantarum (Lactobacillus plantarum) XH, and preserving number is
CGMCC NO.11763, the preparation method of lactic acid bacteria bacterium powder is will to make in the preparation process of above-mentioned maize oligopeptide
Standby streptococcus acidi lactici fermented solution prepares after rear bacterium mud is freeze-dried by centrifugation.
Remaining raw material is commercially available prod.
2 one kinds of functional foods contributing to liver nutrition improvement of embodiment and preparation method thereof
It is made up of the raw material of following parts by weight: WPC 80 parts, maize oligopeptide 50 parts, DHA
Algae oil-bound distemper 3 parts, taurine 10 parts, grape seed extract 5 parts, maltodextrin 20 parts；
Weigh each component according to above-mentioned formula rate, pulverize, cross 80 mesh sieves, then mix 10min, obtain powder
Agent.
The preparation method of described maize oligopeptide is as follows:
(1) in corn protein powder, add the water of w/v 20 times, mix, adjust pH10, heating
To 80 DEG C of insulated and stirred 60min；Feed liquid is discarded clear liquid after centrifugation operates, continues to employ slag charge；Weight
Multiple above-mentioned steps obtains slag charge 3 times；
(2) water (m:v) adding 15 times in above-mentioned slag charge is sized mixing, and regulates pH10, and temperature 70 C, by 10000U/g
Corn protein powder adds alkali protease, enzymolysis 3h；
(3) regulate pH7, temperature 50 C with streptococcus acidi lactici fermented solution, add neutrality by 15000U/g corn protein powder
Protease, enzymolysis 6h；
(4) regulation pH7, temperature 60 C, adds flavor protease, enzymolysis 5h by 1000U/g corn protein powder；,
After enzymolysis terminates, being heated to 95 DEG C, go out enzyme 20 minutes；
(5), after after 4000r/min centrifugal vacuum concentrates, maize oligopeptide it is spray-dried to obtain；
The preparation method of described streptococcus acidi lactici fermented solution is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access equipped with 50mL culture medium
In the 250mL triangular flask of MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 16h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 15%, and at 38 DEG C, 200rpm cultivates
12 hours, dissolved oxygen controlled 10% (ventilation 0.5L/min), Anaerobic culturel 70 hours the most again；
Described fermentation medium composition is as follows: peptone 12g, beef extract 12g, yeast extract 8g, lemon
Acid hydrogen two ammonium 4g, glucose 25g, Tween 80 2mL, sodium acetate 7g, dipotassium hydrogen phosphate 3g, sulfuric acid
Magnesium 0.6g, manganese sulfate 0.3g, cholesterol 120mg, Chinese herbal medicine powder 8g, distilled water 1 000mL, pH 6.6；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is 12mg/mL
Cholesterol solution, add to the most by a certain percentage in culture medium, making cholesterol ultimate density is 120 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh 20 parts of the fruit of Chinese magnoliavine；Radix Codonopsis 15 parts；Hawthorn 20 parts；Respectively said herbal medicine is crushed to particle diameter
It is less than 2 millimeters, in container, then uniformly mixes and add the water of 6 times of weight, control temperature 60 C and protect
Holding 4h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 7, and enzymolysis 4h finally adds mixed material
3 times of w ethanol and the mixture of propyl alcohol, ultrasonic extraction 1.5h under 110W power, filters；Filter vacuum is dense
Contracting postlyophilization obtains Chinese herbal medicine powder；
Described mixed enzyme addition is the 10% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 20 parts, beta amylase 15 parts, pectase 15 parts,
Acid protease 15 parts, acid phosphatase 10 parts；
The mass ratio of described ethanol and propyl alcohol is 1:2.
The preparation method of described grape seed extract is as follows:
(1) grape pip was pulverized 100 mesh sieves, added water and the lactic acid bacteria bacterium of 0.15% of grape pip weight 30%
Powder, room temperature lower seal ferments 5 days, is dried under vacuum to moisture content≤5%, obtains pre-processing grape pip powder；
(2) pretreatment grape pip powder is placed in 0.5% sodium bicarbonate solution of 5 times of volumes (m:v), regulates pH
Value is 8, is warming up to 50 DEG C, adds the mixed enzyme of pretreatment grape pip opaque amount 8%, stirs, enzymolysis
70min, carries out high-pressure pulse electric process, electric-field intensity 40kV/cm, burst length 500 μ s, arteries and veins during enzymolysis
Rush frequency 300Hz；The enzymolysis liquid of above-mentioned process filters through 300 eye mesh screens, and filtrate is freeze-dried i.e. obtains grape
Seed extract；The quality group of described mixed enzyme becomes: amylase: pectase: cellulase=1:8:10；
Described lactic acid bacteria is specially Lactobacillus plantarum (Lactobacillus plantarum) XH, and preserving number is
CGMCC NO.11763, the preparation method of lactic acid bacteria bacterium powder is will to make in the preparation process of above-mentioned maize oligopeptide
Standby streptococcus acidi lactici fermented solution obtains bacterium mud the most afterwards, freeze-dried rear prepared.
Remaining raw material is commercially available prod；
3 one kinds of functional foods contributing to liver nutrition improvement of embodiment and preparation method thereof
It is made up of the raw material of following parts by weight: WPC 40 parts, maize oligopeptide 30 parts, DHA
Algae oil-bound distemper 2 parts, taurine 5 parts, grape seed extract 3 parts, maltodextrin 40 parts；
Weigh each component according to above-mentioned formula rate, pulverize, cross 80 mesh sieves, then mix 10min, obtain powder
Agent.
The preparation method of described maize oligopeptide is as follows:
(1) in corn protein powder, add the water of w/v 15 times, mix, adjust pH9, heating
To 70 DEG C of insulated and stirred 40min；Feed liquid is discarded clear liquid after 4000rpm centrifugation operates, continues to employ
Slag charge；Repeat the above steps obtains slag charge 2 times；
(2) water (m:v) adding 10 times in above-mentioned slag charge is sized mixing, and regulates pH9, and temperature 60 C, by 9000U/g
Corn protein powder adds alkali protease, enzymolysis 2.5h；
(3) regulate pH6, temperature 40 DEG C with streptococcus acidi lactici fermented solution, add neutrality by 12000U/g corn protein powder
Protease, enzymolysis 5.5h；
(4) regulation pH6.5, temperature 50 C, adds flavor protease, enzymolysis 4h by 800U/g corn protein powder；,
After enzymolysis terminates, being heated to 90 DEG C, go out enzyme 20 minutes；
(5), after 4000r/min centrifugal vacuum concentrates, supernatant is spray-dried to obtain maize oligopeptide；
The preparation method of described streptococcus acidi lactici fermented solution is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access equipped with 50mL culture medium
In the 250mL triangular flask of MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 14h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 12%, and at 36 DEG C, 150rpm cultivates
10 hours, dissolved oxygen controlled 10% (ventilation 0.5L/min), Anaerobic culturel 65 hours the most again；
Described fermentation medium composition is as follows: peptone 11g, beef extract 11g, yeast extract 6g, lemon
Acid hydrogen two ammonium 3g, glucose 22g, Tween 80 1.5mL, sodium acetate 6g, dipotassium hydrogen phosphate 2.5g, sulphur
Acid magnesium 0.6g, manganese sulfate 0.25g, cholesterol 110mg, Chinese herbal medicine powder 6g, distilled water 1 000mL,
pH 6.5；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is 11mg/mL
Cholesterol solution, add to the most by a certain percentage in culture medium, making cholesterol ultimate density is 110 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh 15 parts of the fruit of Chinese magnoliavine；Radix Codonopsis 12 parts；Hawthorn 15 parts；Respectively said herbal medicine is crushed to particle diameter
It is less than 2 millimeters, in container, then uniformly mixes and add the water of 5 times of weight, control temperature 50 C and protect
Holding 3h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6.5, and enzymolysis 3h finally adds mixture
Expect 2 times of w ethanol and the mixture of propyl alcohol, ultrasonic extraction 1h under 110W power, filter；Filter vacuum is dense
Contracting postlyophilization obtains Chinese herbal medicine powder；
Described mixed enzyme addition is the 8% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 18 parts, beta amylase 12 parts, pectase 12 parts,
Acid protease 12 parts, acid phosphatase 8 parts；
The mass ratio of described ethanol and propyl alcohol is 1:2.
The preparation method of described grape seed extract is as follows:
(1) grape pip was pulverized 100 mesh sieves, added water and the lactic acid bacteria bacterium of 0.1% of grape pip weight 25%
Powder, room temperature lower seal ferments 4 days, is dried under vacuum to moisture content≤5%, obtains pre-processing grape pip powder；
(2) pretreatment grape pip powder is placed in 0.4% sodium bicarbonate solution of 5 times of its volumes (m:v), adjusts
Joint pH value is 7.5, is warming up to 45 DEG C, adds the mixed enzyme of pretreatment grape pip opaque amount 5%, stirs,
Enzymolysis 55min, carries out high-pressure pulse electric process during enzymolysis, electric-field intensity 30kV/cm, burst length 450 μ s,
Pulse frequency 250Hz；The enzymolysis liquid of above-mentioned process filters through 200 eye mesh screens, filtrate freeze-dried Ji get Portugal
Grape seed extract；The quality group of described mixed enzyme becomes: amylase: pectase: cellulase=1:6:8；
Described lactic acid bacteria is specially Lactobacillus plantarum (Lactobacillus plantarum) XH, and preserving number is
CGMCC NO.11763, the preparation method of lactic acid bacteria bacterium powder is will to make in the preparation process of above-mentioned maize oligopeptide
Standby streptococcus acidi lactici fermented solution obtains bacterium mud the most afterwards, prepares after bacterium mud freeze-drying.
Remaining raw material is commercially available prod.
Embodiment 4 present invention effect to subacute alcoholic hepatic injury
Adult male mice (18-22 gram), often group 15.
With embodiment 3 products obtained therefrom as laboratory sample, if three dosage groups, set blank group, model simultaneously
Control group and negative control group (negative control 1: prepared by maize oligopeptide in embodiment 3 product preparation process
Middle streptococcus acidi lactici fermented solution acid adjustment is adjusted to hydrochloric acid acid adjustment, and the product of the constant production of other steps is as negative control 1；
It is adjusted to grape seed extract preparation in embodiment 3 product preparation process is added lactobacillus-fermented pretreatment
Extracting after directly pulverizing, the product of the constant production of other steps is as negative control 2), in addition to blank group,
Often group presses 10ml/kg body weight gavage 30% ethanol, and after 1h, often group all supplies according to 0.1ml/10g body weight gavage
Trial product sample liquid (prepared by sample liquid: 2g respectively organizes test specimen and adds 50mL ultra-pure water, vibration mixing, to obtain final product),
Blank group and model group give the physiological saline alcohol of same volume, once a day, continuous 30d.Experiment terminates front taboo
Food 4h, after the Nembutal sodium solution of lumbar injection 60mg/kg BW is anaesthetized, abdominal aorta is taken a blood sample, and
Take hepatic tissue, carry out detection and the histopathologic examination of indices.
(1) body weight, CHOL, LDL and TBIL measure
It is respectively adopted Zhongsheng Beikong Biological Science & Technology Co., Ltd.'s cholesterol determination kit (COD-PAP), on
Hai Kexin biotechnology research institute LDL-C kit (direct measuring method), middle raw north control biology
Science and Technology Co., Ltd.'s total bilirubin determination reagent kit (heavy nitrogen) measures CHOL, LDL and TBIL in serum
Content.
Mouse original body mass respectively organized by table 1 and whole opisthosoma heavily comparesN=15
| Group | Product dosage (mg/Kg, wt, d) | Original body mass (g) | Whole opisthosoma weight (g) |
| Blank group | 0 | 22.3±1.2 | 26.4±0.9 |
| Model control group | 0 | 22.1±1.5 | 23.8±1.2 |
| Dosage 1 group | 200 | 22.2±1.7 | 24.9±1.1 |
| Dosage 2 groups | 600 | 21.9±1.3 | 25.8±0.8 |
| Dosage 3 groups | 1000 | 22.1±1.4 | 26.2±1.4 |
| Negative control group 1 | 600 | 22.5±0.9 | 25.5±1.2 |
| Negative control group 2 | 600 | 22.0±1.1 | 24.3±0.8 |
Table 2 present invention impact on mice serum CHOL, LDL, TBIL contentN=15
Note: * P < 0.05, * * P < 0.01, compared with model group
Result judges:
From table 1, model group relatively blank group Body weight loss at the end of experiment is obvious, and is using this
After invention product, the experimental group of various dose all creates the effect that body weight is gone up, and illustrates that this product has raising and exempts from
Effect is obvious and dosage is moderate for the effect of epidemic disease power, particularly dosage 2 groups.Data by negative control group 2 are permissible
Find out, grape seed extract preparation process is added lactic acid bacteria and is favorably improved product enhancing immunity of organisms
Effect.
From table 2, model group relatively blank group is change of serum C HOL, LDL, TBIL at the end of experiment
Content the most substantially rises, and the experimental group of various dose all creates obvious effect after using product of the present invention,
Particularly effect is notable and dosage is moderate for dosage 2 groups.
The data explanation of negative control group 1, the effect of lactic acid bacteria can improve this product relieving alcoholic liver injury
Effect.Negative control group 2 data explanation, use ferment in advance carry out grape seed extract preparation contribute to
Improve product and strengthen the effect of immunity of organisms.
(2) liver pathomorphology change, diagnostic criteria and result judge
Take mouse liver lobus sinister to fix with 10% formalin, in the middle part of left lobe of liver, do cross section draw materials, conventional system
Sheet, HE dyes.With whole histotomy of 5 times of object lens Continuous Observations, main detection degeneration of liver cells (fat
Sex change, hydropic degeneration, endochylema cohesion, balloon sample become), necrosis of liver cells and inflammation change etc., and give simultaneously
Give record.
Standards of grading:
Ballooning degeneration of liver cells:
Hepatic cell fattydegeneration:
Endochylema condenses:
Hydropic degeneration:
Inflammation changes:
Necrosis of liver cells:
Hepatic disease point system:
Every animal liver cell various pathological change score being added, necrosis of liver cells is marked 2 times and is counted, with always
Divide and carry out statistical analysis.(balloon sample becomes point+fat for every animal's liver pathology score=degeneration of liver cells score
Sex change score+endochylema cohesion score+hydropic degeneration score) to change score × 1+ liver thin for × 1+ liver cell inflammation
Born of the same parents' necrosis score × 2.
Pathological examination:
Table 3 present invention impact on mouse liver changes in histopathology
Note: * P < 0.05, * * P < 0.01, compared with model group
Result:
From table 3, model group relatively blank group terminates liver pathology characteristic in experiment and deteriorates serious, and
After using product of the present invention, the experimental group of various dose all creates obvious effect, particularly dosage 2 groups effect
Fruit is notable and dosage is moderate.And the data explanation of negative control group, the access of lactic acid bacteria acid adjustment mode can effectively carry
The effect of high relieving alcoholic liver injury of the present invention.
Claims (8)
1. contribute to a functional food for liver nutrition improvement, be made up of the raw material of following parts by weight: WPC 10-80
Part, maize oligopeptide 5-50 part, DHA algal oil-bound distemper 0.2-3 part, taurine 1-10 part, grape seed extract 0.2-5 part, wheat
Bud dextrin 10-80 part；
The preparation method of described maize oligopeptide is as follows:
(1) in corn protein powder, add the water of w/v 10-20 times, mix, adjust pH8-10, be heated to 50-80 DEG C
Insulated and stirred 20-60min；Feed liquid is discarded clear liquid after centrifugation operates, continues to employ slag charge；Repeat the above steps 1-3
Secondary slag charge；
(2) water adding w/v 8-15 times in above-mentioned slag charge is sized mixing, and regulates pH8-10, temperature 50-70 DEG C, presses
8000-10000U/g corn protein powder adds alkali protease, enzymolysis 2-3h；
(3) regulate pH5-7, temperature 30-50 DEG C with streptococcus acidi lactici fermented solution, add neutral egg by 10000-15000U/g corn protein powder
White enzyme, enzymolysis 5-6h；
(4) regulation pH6-7, temperature 40-60 DEG C, add flavor protease, enzymolysis 3-5h by 400-1000U/g corn protein powder；,
After enzymolysis terminates, being heated to 85 DEG C-95 DEG C, go out enzyme 15-20 minute；
(5), after centrifugal concentrating, it is spray-dried.
A kind of functional food contributing to liver nutrition improvement, it is characterised in that described grape pip extracts
The preparation method of thing is as follows:
(1) grape pip is pulverized, add water and the lactic acid bacteria bacterium powder of 0.05%-0.15%, the room temperature of grape pip weight 20%-30%
Lower seal ferments 3-5 days, is dried under vacuum to moisture content≤5%, obtains pre-processing grape pip powder；
(2) being placed in 0.3-0.5% sodium bicarbonate solution by pretreatment grape pip powder, regulation pH value is 7-8, is warming up to 40-50 DEG C,
The mixed enzyme of addition pretreatment grape pip opaque amount 2-8%, stirs, enzymolysis 40-70min, carries out high-voltage pulse during enzymolysis
Electric field treatment, electric-field intensity 20-40kV/cm, burst length 400-500 μ s, pulse frequency 200-300Hz；The enzyme of above-mentioned process
Solving liquid to filter through 100-300 eye mesh screen, filtrate is freeze-dried i.e. obtains grape seed extract；The quality group of described mixed enzyme becomes:
Amylase: pectase: cellulase=1:5-8:5-10.
A kind of functional food contributing to liver nutrition improvement, it is characterised in that described lactic acid bacteria
Being specially Lactobacillus plantarum (Lactobacillus plantarum) XH, preserving number is CGMCC NO.11763.
A kind of functional food contributing to liver nutrition improvement, it is characterised in that described lactobacillus-fermented
The preparation method of liquid is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access equipped with 50mL culture medium MRS culture medium
In 250mL triangular flask, 200rpm, cultivates 12-16h, makes thalline be in mid log phase for 37 DEG C；
(2) accessing in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is that at 10-15%, 35-38 DEG C, 100-200rpm cultivates 8-12
Hour, dissolved oxygen controls 10%, Anaerobic culturel 60-70 hour the most again.
A kind of functional food contributing to liver nutrition improvement, it is characterised in that described fermentation medium
Form as follows: peptone 10-12g, beef extract 10-12g, yeast extract 5-8g, diammonium hydrogen citrate 2-4g, glucose
20-25g, Tween 80 1-2mL, sodium acetate 5-7g, dipotassium hydrogen phosphate 2-3g, magnesium sulfate 0.58-0.6g, manganese sulfate 0.25-0.3
G, cholesterol 100-120mg, Chinese herbal medicine powder 5-8g, distilled water 1000mL, pH 6.2～6.6；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is that the courage of 10-12mg/mL is solid
Alcoholic solution, adds in culture medium the most by a certain percentage, and making cholesterol ultimate density is 100-120 μ g/mL.
A kind of functional food contributing to liver nutrition improvement, it is characterised in that described Chinese herbal medicine powder
Preparation method as follows:
Weigh fruit of Chinese magnoliavine 10-20 part；Radix Codonopsis 10-15 part；Hawthorn 10-20 part；It is 2 that said herbal medicine is crushed to particle diameter respectively
Below Hao meter, in container, then uniformly mix and add the water of 3-6 times of weight, control temperature 45-60 DEG C and keep 2～4h, add
Entering mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6-7, enzymolysis 2-4h, finally adds 0.5-3 times of w ethanol of mixed material
With the mixture of propyl alcohol, ultrasonic extraction 0.5～1.5h under 110W power, filter；Filter vacuum concentrates in postlyophilization acquisition
Herbal medicine pulvis；
Described mixed enzyme addition is the 5-10% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 15-20 part, beta amylase 10-15 part, pectase 10-15 part,
Acid protease 10-15 part, acid phosphatase 5-10 part；
The mass ratio of described ethanol and propyl alcohol is 1:1-2.
7. contribute to a functional food for liver nutrition improvement as claimed in claim 1, be made up of the raw material of following parts by weight:
WPC 40 parts, maize oligopeptide 30 parts, DHA algal oil-bound distemper 2 parts, taurine 5 parts, grape seed extract 3 parts,
Maltodextrin 40 parts.
8. described in claim 1, contribute to the functional food application in healthcare field of liver nutrition improvement.
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| CN106579464A (en) * | 2016-11-03 | 2017-04-26 | 首都医科大学附属北京佑安医院 | Liver cirrhosis treating nutrition composition and preparation method thereof |
| CN106617109A (en) * | 2016-11-09 | 2017-05-10 | 北京东方兴企食品工业技术有限公司 | Functional nutrition composition for improving and treating liver disease and preparation method of functional nutrition composition |
| CN106820115A (en) * | 2017-01-06 | 2017-06-13 | 北京农品堂食品有限公司 | A kind of plant energy rod suitable for sport people and preparation method thereof |
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| CN115669930A (en) * | 2022-11-07 | 2023-02-03 | 厦门和美科盛生物技术有限公司 | Functional product for dispelling effects of alcohol and protecting liver based on lactobacillus plantarum fermentation |
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