CN105746584A - Insecticidal composition containing metarhizium anisopliae and cyromazine and preparation method and application of insecticidal composition - Google Patents
Insecticidal composition containing metarhizium anisopliae and cyromazine and preparation method and application of insecticidal composition Download PDFInfo
- Publication number
- CN105746584A CN105746584A CN201610196602.8A CN201610196602A CN105746584A CN 105746584 A CN105746584 A CN 105746584A CN 201610196602 A CN201610196602 A CN 201610196602A CN 105746584 A CN105746584 A CN 105746584A
- Authority
- CN
- China
- Prior art keywords
- cyromazine
- agent
- metarhizium anisopliae
- grams
- pesticidal combination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/66—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms
- A01N43/68—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms with two or three nitrogen atoms directly attached to ring carbon atoms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an insecticidal composition containing metarhizium anisopliae and cyromazine and a preparation method and application of the insecticidal composition.According to the insecticidal composition, metarhizium anisopliae and cyromazine serve as basic active ingredients, and metarhizium anisopliae conidia (piece) and cyromazine (gram) are mixed according to the ratio of (49-1) *2 *1010:(1-49).Wet powder, water dispersible granules and an oil suspending agent can be prepared after auxiliary materials are added to the active ingredients.The insecticidal composition can be used for preventing and treating pests such as scale insects, trialeurodes vaporariorum, thrips, grubs, wireworms, citrus fruit flies, liriomyza sativae, root maggots and Bradysia odoriphaga Yang et Zhang, is especially suitable for preventing and treating diptera pests such as citrus fruit flies, liriomyza sativae, root maggots and bradysia odoriphaga, and has the advantages of being stable in pesticide effect, good in fast-acting property, long in lasting period, safe, environmentally friendly and the like.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of containing Metarhizium anisopliae and the insecticides of cyromazine and
Preparation method and application.
Background technology
Metarhizium anisopliae (Metarhizium anisopliae) is the insect pathogenic fungus of a kind of wide spectrum, has distribution big,
The features such as parasitic wide spectrum, to people, animal and crop nonhazardous, can parasitic various insects, be to study on our times and apply at most
One of entomogenous fungi.According to incompletely statistics, 200 various insects such as whole world Diptera, Lepidoptera, coleoptera, Homoptera
Can be lethal by this fungal infection.Green muscardine fungus has a significant insecticidal effect, and has and be different from the brand-new of existing insecticide
The mechanism of action, mainly acts on insect hemolymph immune system, is allowed to lose immune defense function.The whole world research to green muscardine fungus
The history of existing more than 100 year, at present, domestic existing Metarhizium anisopliae bacterial strain develops into insecticide, is mainly used in preventing and treating beach
/ grassland grasshopper, Chinese cabbage beet armyworm, apple tree small heart-eating peach worm.But owing to by the most multifactorial restriction, causing its land for growing field crops
Actual preventive effect is unstable, greatly constrains its large-scale production and application.Exploitation Metarhizium anisopliae compounded pesticides, needs badly
Overcome its prevention effect unstable and the slow defect that takes effect, to preventing and treating subterranean pest-insect and Fruit fly, significant.
Cyromazine belongs to 1,3,5-triazines class insect growth regulator, IGR, and Diptera larvae is had sp act, has strong Uptake and translocation
Effect.Luring that Diptera larvae and pupa are morphologically distorted into, adult eclosion is the most complete or suppressed, can be used for prevent and treat liriomyza bryoniae,
The insects such as citrus fruit fly, root maggot, Bradysia odoriphaga, have preferable activity to larva and pupa, to the prevention effect of adult relatively
Difference, is used alone for a long time, insect can be caused to develop immunity to drugs.
Folium Allii tuberosi root maggot formal name used at school is Bradysia fungus gnat Bradysia odoriphaga Yang et Zhang, also referred to as " fragrant-flowered garlic maggot ", is to endanger me
The primary pest of state Specialty vegetable Folium Allii tuberosi.Fragrant-flowered garlic maggot cluster harm Folium Allii tuberosi underground rhizome, causes blade to rot, and Folium Allii tuberosi is withered, seriously
Affect Folium Allii tuberosi yield and quality.Produce upper conventional chemical pesticide such as Bayer 71628, parathion, chlopyrifos, phoxim pellet etc. at present to enter
Row pouring root is prevented and treated, and even have uses the preventing and treating of high-toxic pesticide thimet, causes Folium Allii tuberosi Pesticide Residues to exceed standard, and China is once on mountain
" the poison Folium Allii tuberosi event " that east and Henan occur is exactly that thus the health of danger Xie Zhe consumer is good for caused by highly toxic pesticide preventing and treating fragrant-flowered garlic maggot
Health, foreign exchange earning also receives the biggest impact.
Using pesticide combination technology or mixing application technique is to overcome or delay a drug-fast important method, affects sloughing off of insecticide
Skin hormone, reduces the body weight of insecticide, suppresses its motor capacity, is conducive to improving the Metarhizium anisopliae infection rate to insect, carries
High insecticide efficiency.Not yet it is found to have the compounded pesticides of relevant Metarhizium anisopliae and cyromazine at present.
Summary of the invention
It is an object of the invention to overcome prior art not enough, it is provided that a kind of Pesticidal combination, this Pesticidal combination is pollution-free, low residual
Stay, safety and environmental protection, multiple worm state is respectively provided with preferable prevention effect.
The present invention additionally provides preparation and the application of this Pesticidal combination simultaneously.
The present invention is achieved through the following technical solutions:
A kind of Pesticidal combination of the present invention, containing Metarhizium anisopliae (Metarhizium anisopliae) and cyromazine, described
Metarhizium anisopliae is mixed in following ratio with described cyromazine: the conidial number of described Metarhizium anisopliae and described fly eradication
The grams of amine is than being (49-1) × 2 × 1010: (1-49).
Preferably, described compositions is made up of Metarhizium anisopliae, cyromazine and adjuvant.
The present invention with Metarhizium anisopliae and cyromazine as host, add adjuvant can be processed into wettable powder, water dispersible granules,
The preparations such as oil-suspending agent.Described adjuvant changes because the dosage form of processing is different.
Preferably, described adjuvant is wetting agent, dispersant, stabilizer and carrier, and the weight of each component of described adjuvant accounts for described
The percent of the gross weight of Pesticidal combination is respectively as follows: wetting agent and accounts for 5-35%, and dispersant accounts for 5-30%, and stabilizer accounts for 0%-5%,
Carrier complements to 100%；Described wetting agent is TERWET1004, dodecylbenzene sodium sulfonate, alkylnaphthalene sulfonate with cloudy from
Sub-humectant mixture (MorwetEFW), tea seed cake, spaonin powder, Fructus Sapindi Mukouossi powder, Bombyx mori L. and one or more pulled open in powder；
Described dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensation substance sulphur
One or more of hydrochlorate, fatty alcohol-polyoxyethylene ether, nekal and lignosulfite；Described stabilizer is
One in butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate and tert-butylhydroquinone or
Multiple；Described carrier is one or more in Kaolin, white carbon, attapulgite, kieselguhr and bentonite.
Above-mentioned adjuvant is processed into wettable powder, by cyromazine, wetting agent, dispersant, carrier, stabilizer mixing, at premix
Mix homogeneously in tank, prepares micropowder after jet mill is pulverized, mixs homogeneously with Metarhizium anisopliae spore powder.
Preferably, described adjuvant is wetting agent, dispersant, disintegrating agent, binding agent, stabilizer and carrier；Described adjuvant each
The percent of the gross weight that the weight of component accounts for described Pesticidal combination is respectively as follows: wetting agent and accounts for 1-15%, and dispersant accounts for 2-20%,
Disintegrating agent accounts for 1-18%, and binding agent accounts for 1-5%, and stabilizer accounts for 0%-5%, and carrier complements to 100%；Described wetting agent is polyethers
Modified polyorganosiloxanes, sodium lauryl sulphate, fatty acid sulfates, fatty alcohol-polyoxyethylene ether, alkyl phenolic group polyoxy second
One or more in alkene ether；Described dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalenesulfonateformaldehyde formaldehyde condensation
In thing, alkyl naphthalene formaldehyde condensate sulfonates, fatty alcohol-polyoxyethylene ether, nekal and lignosulfite
One or more；Described disintegrating agent is sodium bicarbonate, bentonite, ammonium sulfate, carbamide, magnesium sulfate, sodium carbonate and aluminum chloride sugarcane
One or more in sugar；Described binding agent be paraffin oil, Polyethylene Glycol, polyvinyl alcohol, cyclodextrin, starch, sodium silicate,
One or more in phenolic resin and methylcellulose；Described stabilizer be butylated hydroxyarisol, 2, the tertiary fourth of 6-bis-
One or more in base paracresol, propylgallate and tert-butylhydroquinone；Described carrier be Kaolin, white carbon,
One or more in attapulgite, kieselguhr and bentonite.
Above-mentioned adjuvant is processed into water dispersible granules, weighs Metarhizium anisopliae height cryptogam, cyromazine, wetting agent, dispersant, collapses
After solution agent, stabilizer, carrier proportionally mix coarse pulverization, pulverize with jet mill and prepare micropowder, by gained micropowder
In fluidized bed pelletizer, hybrid adhesive carries out pelletize, carries out sieving to obtain product through vibrosieve after being dried.
Preferably, described adjuvant is wetting agent, emulsifying agent, stabilizer and oil base, and described adjuvant is wetting agent, emulsifying agent, steady
Determining agent and oil base, the percent of the gross weight that each component of described adjuvant accounts for described Pesticidal combination is respectively as follows: wetting agent and accounts for
1-15%, emulsifying agent accounts for 1-15%, and stabilizer accounts for 0%-5%, and oil base supplies 100%；Described wetting agent is polyether-modified poly-organic
In siloxanes, sodium lauryl sulphate, fatty acid sulfates, fatty alcohol-polyoxyethylene ether and alkyl phenolic group polyoxyethylene ether
One or more；Described emulsifying agent be sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol with
Ethylene oxide condensate, polyoxyethylene fatty acid ester, triethanolamine list olein and Oleum Ricini and in ethylene oxide condensate
Plant or several；Described stabilizer is dibenzylatiooluene, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl
One or more in hydroquinone；Described oil base is Oleum Brassicae campestris, Oleum sesami, Oleum Arachidis hypogaeae semen, Oleum Gossypii semen, salad oil and paraffin oil
In one or more.
Above-mentioned adjuvant is processed into oil-suspending agent, and cyromazine, Ricinate, emulsifying agent, stabilizer, oil base are pressed the ratio of formula
Example is sequentially added in mixing kettle, stirs, and enters sand mill and carry out sand milling after high shear dispersion still is sheared, sand milling to 90%
Particle diameter i.e. obtains oil-suspending agent product less than mixing homogeneously with muscardine spore powder after 5 microns.
The dosage form of described compositions is wettable powder, water dispersible granules or oil-suspending agent.
Preferably, the dosage form of described compositions is oil-suspending agent, the conidial number of described Metarhizium anisopliae and described fly eradication
The grams ratio of amine is 9 × 2 × 1010: 1.
Above-mentioned Pesticidal combination can be applicable to prevent and treat scale insect, trialeurodes vaporariorum, thrips, Holotrichia diomphalia Bates, wireworm, citrus fruit fly, speckle dive
In fly, root maggot, fragrant-flowered garlic maggot and Bradysia odoriphaga.
The present composition is particularly suitable for the preventing and treating harm various root maggots of foot end and Folium Allii tuberosi maggot, applicable place or
Plant includes cotton, Semen Maydis, potato class, beans, melon, brassicaceous vegetable, Herba Spinaciae, Herba Alii fistulosi, Bulbus Allii, Folium Allii tuberosi etc..Application method
Spraying, pouring can be taked to fill, spread fertilizer over the fields, and above application method all can be combined with the most mixed soil phase.The phase is contained at adult eclosion,
Spray field furrow with the present composition, adult can be killed.When finding larva, ovum and larva can be killed by present composition pouring root.
Formulation rate is according to different with the condition such as soil, crop determining through simple experiment in worm kind, insect density, worm age.
Muscardine spore in the Pesticidal combination of the present invention is good with the compatibility of cyromazine, has obvious potentiation, with list
Agent pesticide is compared, and insecticidal toxicity significantly improves, efficacy stability, and quick-acting is good；The usage amount of chemical pesticide reduces, environmental pollution
Reduce；The present invention reduces the usage amount of green muscardine fungus, reduces production cost；Two kinds of diverse farm chemical ingredients of mechanism of action are entered
Row is compounding, adds target site, makes insecticide be not likely to produce Drug resistance；Compositions after Fu Pei to the ovum of insect, larva, pupa,
Adult all has preferable prevention effect.
Detailed description of the invention
For making the present invention easier to understand, below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these are real
Execute example be merely to illustrate the present invention rather than limit the scope of the present invention, NM specific experiment method in the following example,
Generally carry out according to normal experiment method.
Percentage ratio in embodiment refers both to percentage by weight, and described muscardine spore refers both to Metarhizium anisopliae conidium, described
The amount of cyromazine is effective dose, converts according to former medicine content used during actual preparation, and the amount of deviation passes through filling adding amount
It is adjusted.
The embodiment of the present invention is the method using indoor virulence test and field test to combine.
Embodiment 1: Metarhizium anisopliae cyromazine wettable powder (the conidial number of Metarhizium anisopliae and described fly eradication
The grams ratio of amine is 39 × 2 × 1010: 1)
Weigh Metarhizium anisopliae spore powder 390 grams, cyromazine 10.2 grams, wetting agent dodecylbenzene sodium sulfonate 105 grams, soap
150 grams of angle powder, dispersant lignosulfonates 50 grams, modified lignin mahogany sulfonate 150 grams, the stabilizer tert-butyl group is to hydroxyl fennel
Fragrant 12 grams of ether, surplus is that to mend ad pond om as carrier be 1000 grams to kieselguhr.
By described cyromazine, described wetting agent, described dispersant, described stabilizer and described carrier mix homogeneously in premixing tank,
After jet mill is pulverized, prepare micropowder, mix homogeneously with Metarhizium anisopliae spore powder, obtain wettable powder parasite killing group
Compound.
In a further advantageous embodiment, being formulated as Metarhizium anisopliae cyromazine wettable powder, wherein Metarhizium anisopliae divides
Sporogenic number is 49 × 2 × 10 with the grams ratio of described cyromazine10: 1.Weigh Metarhizium anisopliae spore powder 490 grams, go out
Fly amine 10.2 grams, wetting agent TERWET1004 100 grams, dispersant naphthalene sulfonic acid-formaldehyde condensation product 105 grams, alkyl naphthalene formaldehyde
Condensate sulfonates 95 grams, stabilizer 2,6 ditertiary butyl p cresol 20 grams, surplus is that attapulgite is mended to gross weight as carrier
Amount is 1000 grams.
In a further advantageous embodiment, being formulated as Metarhizium anisopliae cyromazine wettable powder, wherein Metarhizium anisopliae divides
Sporogenic number is 1 × 2 × 10 with the grams ratio of described cyromazine10: 1.Weigh Metarhizium anisopliae spore powder 10 grams, fly eradication
Amine 10.2 grams, wetting agent alkylnaphthalene sulfonate and anion humectant mixture MorwetEFW 105 grams, 35 grams of Fructus Sapindi Mukouossi powder,
Dispersant fatty alcohol-polyoxyethylene ether 120 grams, stabilizer tert-butylhydroquinone 10 grams, butylated hydroxyarisol 10 grams,
Surplus is Kaolin, and to mend ad pond om as carrier be 400 grams.
In a further advantageous embodiment, being formulated as Metarhizium anisopliae cyromazine wettable powder, wherein Metarhizium anisopliae divides
Sporogenic number is 1 × 2 × 10 with the grams ratio of described cyromazine10: 49.Weigh Metarhizium anisopliae spore powder 10 grams, fly eradication
Amine 499.8 grams, wetting agent Bombyx mori L. 105 grams, pulls open 100 grams of powder, tea seed cake 50 grams, dispersant nekal 105 grams,
Lignosulfite 150 grams, surplus is that to mend ad pond om as carrier be 5100 grams for white carbon and bentonite.
Embodiment 2: Metarhizium anisopliae cyromazine oil-suspending agent (the conidial number of Metarhizium anisopliae and described fly eradication
The grams ratio of amine is 9 × 2 × 1010: 1).
Weigh Metarhizium anisopliae 20,000,000,000 spores/g height cryptogam 90 grams, cyromazine 10.2 grams, emulsifying agent Sorbitan alcohol ester
Fat acid ester polyoxyethylene ether 100 grams, wetting agent sodium lauryl sulphate 50 grams, stabilizer tert-butylhydroquinone 30 grams, oil
Base Oleum Arachidis hypogaeae semen 450 grams and Oleum sesami 499.8 grams.
Described cyromazine, described wetting agent, described emulsifying agent, described stabilizer and described oil base are sequentially added in mixing kettle,
Stir, after high shear dispersion still is sheared, enter sand mill carry out sand milling, sand milling to 90% particle diameter less than after 5 microns with institute
State muscardine spore powder mix homogeneously, obtain oil-suspending agent disinfestation composition.
In another embodiment, component is formulated as Metarhizium anisopliae 20,000,000,000 spores/g height cryptogam 90 grams, cyromazine 10.2
Gram, emulsifier sorbitol acid anhydride list olein 150 grams, the polyether-modified polysiloxane of wetting agent 150 grams, oil base Oleum Brassicae campestris 450
Gram and Oleum Gossypii semen 149.8 grams.
In another embodiment, component is formulated as Metarhizium anisopliae 20,000,000,000 spores/g height cryptogam 90 grams, cyromazine 10.2
Gram, emulsifying agent fatty alcohol and ethylene oxide condensate 5 grams, polyoxyethylene fatty acid ester 5 grams, wetting agent fatty acid sulfates 5
Gram, fatty alcohol-polyoxyethylene ether 5 grams, stabilizer dibenzylatiooluene 20 grams, 2,6 ditertiary butyl p cresol 30 grams,
Oil base salad oil 829.8 grams.
In another embodiment, component is formulated as Metarhizium anisopliae 20,000,000,000 spores/g height cryptogam 90 grams, cyromazine 10.2
Gram, emulsifying agent triethanolamine list olein 50 grams, Oleum Ricini and ethylene oxide condensate 50 grams, wetting agent alkyl phenolic group polyoxy
Vinyl ethers 100 grams, stabilizer propylgallate 30 grams, oil base paraffin oil 450 grams and 219.8 grams.
Embodiment 3: Metarhizium anisopliae cyromazine water dispersible granules (go out with described by the conidial number of Metarhizium anisopliae
The grams ratio of fly amine is 1 × 2 × 1010: 9)
Weigh Metarhizium anisopliae 20,000,000,000 spores/g cryptogam 10 grams, cyromazine 92 grams, the polyether-modified poly-organosilicon of wetting agent
120 grams of oxygen alkane, dispersant alkyl naphthalene formaldehyde condensate sulfonates 100 grams, fatty alcohol-polyoxyethylene ether 80 grams；Disintegrating agent sulphuric acid
Ammonium 60 grams, adhesive starch 10 grams, stabilizer butylated hydroxyarisol 10 grams, surplus is that kieselguhr is supplied as carrier
Gross weight is 1000 grams.
By described Metarhizium anisopliae spore powder, described cyromazine, described wetting agent, described dispersant, described disintegrating agent, institute
State stabilizer and after described carrier proportionally mixes coarse pulverization, pulverize with jet mill and prepare micropowder, by gained micropowder
In fluidized bed pelletizer, mix described binding agent carry out pelletize, sieve through vibrosieve after being dried, obtain water dispersible granules and kill
Worm compositions.
In another embodiment, component be formulated as Metarhizium anisopliae 20,000,000,000 spores/g cryptogam 10 grams, cyromazine 92 grams,
Wetting agent sodium lauryl sulphate 10 grams, dispersant lignosulfonates 10 grams, modified lignin mahogany sulfonate 10 grams, disintegrating agent
Sodium bicarbonate 5 grams, bentonite 5 grams, binding agent paraffin oil 5 grams, surplus is that to supply gross weight as carrier be 1000 to Kaolin
Gram.
In another embodiment, component be formulated as Metarhizium anisopliae 20,000,000,000 spores/g cryptogam 10 grams, cyromazine 92 grams,
Wetting agent fatty acid sulfates 30 grams, fatty alcohol-polyoxyethylene ether 120 grams, dispersant naphthalene sulfonic acid-formaldehyde condensation product 200 grams；Collapse
Solution 120 grams of agent carbamide, 60 grams of magnesium sulfate, binding agent Polyethylene Glycol 10 grams, polyvinyl alcohol 30 grams, cyclodextrin 10 grams, surely
Determining agent 2,6 ditertiary butyl p cresol 30 grams, propylgallate 20 grams, surplus is that white carbon, attapulgite are as carrier
Supplying gross weight is 1000 grams.
In another embodiment, component be formulated as Metarhizium anisopliae 20,000,000,000 spores/g cryptogam 10 grams, cyromazine 92 grams,
Wetting agent alkyl phenolic group polyoxyethylene ether 80 grams, dispersant nekal 50 grams, lignosulfite 50 grams；
Disintegrating agent sodium carbonate 40 grams, 30 grams of aluminum chloride, sucrose 30 grams, binding agent sodium silicate 5 grams, 5 grams of phenolic resin and methyl are fine
Dimension element 10 grams, stabilizer tert-butylhydroquinone 20 grams, surplus is that to supply gross weight as carrier be 1000 grams to bentonite.
Embodiment 4: the embodiment of the present invention is the method using indoor virulence test and field test to combine.
Test reagent: Metarhizium anisopliae 20,000,000,000 live spore/gram, the former medicine of cyromazine 98%.
Subjects:
With the medicinal liquid of variable concentrations, filter paper is moistened, is layered in 9cm culture dish, Folium Allii tuberosi (white portion) is cut into 2cm segment,
15s is soaked in variable concentrations medicinal liquid.Take out 5 sections to be placed in culture dish, then move into of the same size three with brush pen lightly
Instar larvae.Often process 20, be repeated 4 times, set clear water comparison simultaneously, supplement clear water with manual sprayer simultaneously, keep filter paper
Moistening, supplement the Folium Allii tuberosi stem of dipped respective concentration medicinal liquid every day in time, finally calculate mortality rate.
Carry out statistical analysis with SPSS data processing software, calculate the LC50 of each medicament, evaluate the activity of reagent agent with this.
The co-toxicity coefficient (CTC value) of mixture is calculated by following equation:
In formula:
ATI-mixture actual measurement toxicity index；
The LC50 of S-standard pesticide agent, unit is milligrams per liter；
The LC50 of M-mixture, unit is milligrams per liter.
TTI=TIA × PA+TIB × PB
In formula:
TTI mixture theory toxicity index；
TIA A medicament toxicity index；
PA A medicament percentage composition in mixture, unit is percentage rate；
TIB B medicament toxicity index；
PB B medicament percentage composition in mixture, unit is percentage rate.
In formula:
CTC co-toxicity coefficient；
AT mixture actual measurement toxicity index；
TTI mixture theory toxicity index.
The co-toxicity coefficient (CTC) of built agent:
CTC >=120 show as potentiation；
CTC≤80 show as antagonism；
80 ＜ CTC ＜ 120 show as summation action.
Table 1 is that the difference of the Pesticidal combination of the Metarhizium anisopliae (A) containing different proportion of the present invention and cyromazine (B) is for reagent
Activity (the LC of agent50), co-toxicity coefficient (CTC) and the testing result of mixture theory toxicity index (TTI).Table 1 is indoor poison
Power measurement result, by table 1 result it can be seen that the mass ratio of Metarhizium anisopliae height spore powder and cyromazine is (49-1):
(1-49) obvious potentiation is all shown, wherein with Metarhizium anisopliae height spore powder: cyromazine (mass ratio)=1:
49 synergistic effects are the most obvious.
Virulence significantly improves, efficacy stability, and quick-acting is good；The usage amount of chemical pesticide reduces, and the usage amount of green muscardine fungus reduces；
Insecticide is not likely to produce Drug resistance；The prevention effect that the ovum of insect, larva, pupa, adult have all been had.
Table 1
Embodiment 5: storage spore Activity determination
Peptone potato dextrose agar (PPDA) is applied directly on microscope slide, then takes a little mitogenetic containing green muscardine fungus
Compositions in the embodiment of the present invention 1,2 and 3 of spore powder, is diluted to 1 × 10 with the sterilized water containing 0.1% glycerol7Individual/mL,
Drip uniform application after 20 μ L spore suspension, 28 DEG C of moisturizing 36h.Every kind of spore processing statistics about 300, commonly
Observe and record spore germination situation under microscope 400 times, calculate spore germination rate.Table 2 is spore germination rate result, by this
Table is visible, and the spore activity of Metarhizium anisopliae in compositions is not made by cyromazine and other adjuvant in the present composition
Become significantly impact.
Table 2
| Storage forespore germination rate, % | 180 days spore germination rates of 25 DEG C of indoor storages, % | |
| Example 1 | 95.8 | 88.3 |
| Example 2 | 97.2 | 92.4 |
| Example 3 | 96.3 | 90.7 |
Embodiment 6: Field information is tested:
This experimental establishment is in Tian Liu town, Shouguang City of Shandong Province, and test medicine is researched and developed by Guangdong new scene biological engineering company limited, carried
Confession, compares medicament 10,000,000,000 spore/gram Metarhizium anisopliae wettable powder (commercial), 10% cyromazine suspending agent (commercial).
When Bradysia fungus gnat adult occurs latter stage, water uniformly at Folium Allii tuberosi root after medicament 500 times is diluted, if clear water comparison,
Each process is repeated 4 times.Each plot area 20m2Before dispenser, the Folium Allii tuberosi of each community 1/4 area is grubbed out out, with root
The number of fragrant-flowered garlic maggot as radix, after medicine 7 days, 15 days, 30 days, within 60 days, dig out the Folium Allii tuberosi of 1/4 area again, add up root fragrant-flowered garlic maggot
Number, table 3 is preventive effect result of calculation, and from this table, the present composition is for dose pouring root 7d on probation, 15d, 30d, 60d
Field control effect all reach more than 80%, quick-acting is good, and the lasting period is long, and minimum preventive effect is 80.10%, and the highest preventive effect is
96.10%, effect is substantially better than the prevention effect of single dose.The present composition, while improve preventive effect, reduces dosage.
Table 3
Last institute is it should be noted that, above example is only in order to illustrate technical scheme rather than to scope
Restriction, although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, can
Technical scheme is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention.
Claims (10)
1. one kind contains Metarhizium anisopliae and the Pesticidal combination of cyromazine, it is characterised in that described Pesticidal combination contains Gold Testudinis
Sub-green muscardine fungus and cyromazine, described Metarhizium anisopliae is mixed in following ratio with described cyromazine: described Metarhizium anisopliae
Conidial number is (49-1) × 2 × 10 with the grams ratio of described cyromazine10: (1-49).
Pesticidal combination the most according to claim 1, it is characterised in that described compositions by Metarhizium anisopliae, go out
Fly amine and adjuvant composition.
Pesticidal combination the most according to claim 2, it is characterised in that described adjuvant is wetting agent, dispersant, stablizes
Agent and carrier, the percent of the gross weight that the weight of each component of described adjuvant accounts for described Pesticidal combination is respectively as follows:
Described wetting agent accounts for 5-35%；
Described dispersant accounts for 5-30%；
Described stabilizer accounts for 0%-5%；
Described carrier complements to 100%；
Described wetting agent is TERWET1004, dodecylbenzene sodium sulfonate, alkylnaphthalene sulfonate and anion humectant mixture
MorwetEFW, tea seed cake, spaonin powder, Fructus Sapindi Mukouossi powder, Bombyx mori L. and one or more pulled open in powder；
Described dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensation
One or more in thing sulfonate, fatty alcohol-polyoxyethylene ether, nekal and lignosulfite；
Described stabilizer is that butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate, tertiary butyl are to benzene
One or more in diphenol；
Described carrier is one or more in Kaolin, white carbon, attapulgite, kieselguhr, bentonite.
4. the preparation method of the Pesticidal combination described in claim 3, it is characterised in that by described cyromazine, described wetting agent,
Described dispersant, described stabilizer and described carrier mix homogeneously in premixing tank, prepare micropowder after jet mill is pulverized,
Mix homogeneously with described Metarhizium anisopliae spore powder, obtain wettable powder Pesticidal combination.
Pesticidal combination the most according to claim 2, it is characterised in that described adjuvant is wetting agent, dispersant, collapses
Solve agent, binding agent, stabilizer and carrier；The weight of each component of described adjuvant accounts for the percent of described Pesticidal combination gross weight and divides
It is not:
Described wetting agent accounts for 1-15%；
Described dispersant accounts for 2-20%；
Described disintegrating agent accounts for 1-18%；
Described binding agent accounts for 1-5%；
Described stabilizer accounts for 0%-5%；
Described carrier complements to 100%；
Described wetting agent is polyether-modified polysiloxane, sodium lauryl sulphate, fatty acid sulfates, fatty alcohol polyoxy second
One or more in alkene ether and alkyl phenolic group polyoxyethylene ether；
Described dispersant is lignosulfonates, modified lignin mahogany sulfonate, naphthalene sulfonic acid-formaldehyde condensation product, alkyl naphthalene formaldehyde condensation
One or more in thing sulfonate, fatty alcohol-polyoxyethylene ether, nekal and lignosulfite；
Described disintegrating agent is in sodium bicarbonate, bentonite, ammonium sulfate, carbamide, magnesium sulfate, sodium carbonate and aluminum chloride sucrose
Plant or multiple；
Described binding agent is paraffin oil, Polyethylene Glycol, polyvinyl alcohol, cyclodextrin, starch, sodium silicate, phenolic resin and methyl
One or more in cellulose；
Described stabilizer is butylated hydroxyarisol, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl pair
One or more in Benzodiazepines；
Described carrier is one or more in Kaolin, white carbon, attapulgite, kieselguhr and bentonite.
6. the preparation method of the Pesticidal combination described in claim 5, it is characterised in that by described Metarhizium anisopliae spore powder,
Described cyromazine, described wetting agent, described dispersant, described disintegrating agent, described stabilizer and described carrier proportionally mix
After coarse pulverization, pulverize with jet mill and prepare micropowder, gained micropowder is mixed described binding agent in fluidized bed pelletizer
Carry out pelletize, sieve through vibrosieve after being dried, obtain water dispersible granules Pesticidal combination.
Pesticidal combination the most according to claim 2, it is characterised in that described adjuvant is wetting agent, emulsifying agent, stablizes
Agent and oil base, the percent of the gross weight that the weight of each component of described adjuvant accounts for described Pesticidal combination is respectively as follows:
Described wetting agent accounts for 1-15%；
Described emulsifying agent accounts for 1-15%；
Described stabilizer accounts for 0%-5%；
Described oil base supplies 100%；
Described wetting agent is polyether-modified polysiloxane, sodium lauryl sulphate, fatty acid sulfates, fatty alcohol polyoxy second
One or more in alkene ether and alkyl phenolic group polyoxyethylene ether；
Described emulsifying agent is sorbitan monooleate, sorbitan fatty acid ester polyoxyethylene ether, fatty alcohol and oxirane
Condensation substance, polyoxyethylene fatty acid ester, triethanolamine list olein and Oleum Ricini and one or more in ethylene oxide condensate；
Described stabilizer is that dibenzylatiooluene, 2,6 ditertiary butyl p cresol, propylgallate and tertiary butyl are to benzene two
One or more in phenol；
Described oil base is one or more in Oleum Brassicae campestris, Oleum sesami, Oleum Arachidis hypogaeae semen, Oleum Gossypii semen, salad oil and paraffin oil.
Pesticidal combination the most according to claim 7, it is characterised in that the dosage form of described compositions is oil-suspending agent, institute
The grams ratio stating the conidial number of Metarhizium anisopliae and described cyromazine is 9 × 2 × 1010: 1.
9. the preparation method of the Pesticidal combination described in claim 7, it is characterised in that by described cyromazine, described wetting agent,
Described emulsifying agent, described stabilizer and described oil base are sequentially added in mixing kettle, stir, after high shear dispersion still is sheared
Entering sand mill and carry out sand milling, sand milling to 90% particle diameter is mixed homogeneously with described muscardine spore powder after being less than 5 microns, obtains oil
Suspending agent disinfestation composition.
10. the Pesticidal combination that one of claim 1-9 is described preventing and treating scale insect, trialeurodes vaporariorum, thrips, Holotrichia diomphalia Bates, wireworm,
Application in citrus fruit fly, liriomyza bryoniae, root maggot, fragrant-flowered garlic maggot and Bradysia odoriphaga.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610196602.8A CN105746584B (en) | 2016-03-31 | 2016-03-31 | A kind of Pesticidal combination and its preparation method and application containing Metarhizium anisopliae and cyromazine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610196602.8A CN105746584B (en) | 2016-03-31 | 2016-03-31 | A kind of Pesticidal combination and its preparation method and application containing Metarhizium anisopliae and cyromazine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105746584A true CN105746584A (en) | 2016-07-13 |
| CN105746584B CN105746584B (en) | 2018-10-12 |
Family
ID=56346862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610196602.8A Active CN105746584B (en) | 2016-03-31 | 2016-03-31 | A kind of Pesticidal combination and its preparation method and application containing Metarhizium anisopliae and cyromazine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105746584B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110547304A (en) * | 2019-09-24 | 2019-12-10 | 重庆谷百奥生物研究院有限公司 | Beauveria bassiana and amitraz compounded trialeurodes vaporariorum killing composition |
| WO2020011819A1 (en) * | 2018-07-10 | 2020-01-16 | Bayer Aktiengesellschaft | Novel carrier fluids for liquid fungal spore formulations |
| US12178223B2 (en) | 2020-08-18 | 2024-12-31 | Bergman Industries Ltd | Insect life cycle interruption agent(LCIA) formulations and their manufacture and use |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220142176A1 (en) * | 2019-04-12 | 2022-05-12 | Bayer Cropscience Biologics Gmbh | Methods of increasing the germination rate of fungal spores |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1788569A (en) * | 2005-12-18 | 2006-06-21 | 刘起麟 | Preparation and uses of false proof highly-effective insecticide and bactericide |
| WO2015069708A1 (en) * | 2013-11-08 | 2015-05-14 | Novozymes Bioag A/S | Compositions and methods for treating pests |
-
2016
- 2016-03-31 CN CN201610196602.8A patent/CN105746584B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1788569A (en) * | 2005-12-18 | 2006-06-21 | 刘起麟 | Preparation and uses of false proof highly-effective insecticide and bactericide |
| WO2015069708A1 (en) * | 2013-11-08 | 2015-05-14 | Novozymes Bioag A/S | Compositions and methods for treating pests |
Non-Patent Citations (3)
| Title |
|---|
| SHAO-FENG JIN: "Evaluation of alternative rice planthopper control by the combined action of oil-formulated Metarhizium anisopliae and low-rate buprofezin", 《PEST MANAG. SCI. 第67卷》 * |
| 国家林业局森林病虫害防治总站: "《林业有害生物防治技术》", 31 July 2014 * |
| 迟德富: "《昆虫生长调节剂》", 31 October 2005 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020011819A1 (en) * | 2018-07-10 | 2020-01-16 | Bayer Aktiengesellschaft | Novel carrier fluids for liquid fungal spore formulations |
| CN110547304A (en) * | 2019-09-24 | 2019-12-10 | 重庆谷百奥生物研究院有限公司 | Beauveria bassiana and amitraz compounded trialeurodes vaporariorum killing composition |
| US12178223B2 (en) | 2020-08-18 | 2024-12-31 | Bergman Industries Ltd | Insect life cycle interruption agent(LCIA) formulations and their manufacture and use |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105746584B (en) | 2018-10-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101755847B (en) | Metarhiziumanisopliae compound insecticide composition and use thereof | |
| CN1332610C (en) | Pesticide microemulsion | |
| CN103636660A (en) | Environmental-friendly particulate agent for controlling sugarcane pests | |
| CN104488949A (en) | Dinotefuran-containing insecticidal combination | |
| CN105746584A (en) | Insecticidal composition containing metarhizium anisopliae and cyromazine and preparation method and application of insecticidal composition | |
| CN100563447C (en) | A kind of aqueous pesticide and preparation method thereof | |
| CN101647463A (en) | Insecticidal and acaricidal composition containing spirodiclofen | |
| CN106922728B (en) | A kind of suspension seed-coating agent for preventing brassicaceous vegetable phyllotreta striolata | |
| CN102210314A (en) | Mollusk-killing composition containing niclosamide and carbaryl | |
| CN104115861B (en) | A kind of insecticide acaricide composition containing sulfur-phosphor and cyflumetofen | |
| CN105901007A (en) | Pesticidal composition containing cyclaniliprole and chloropyridine | |
| CN104106593A (en) | Insecticide acaricidal composition containing xiaochongthion and etoxazole | |
| CN104381294A (en) | Insect and mite killing composition containing xiaochongliulin and spirodiclofen | |
| CN104115850A (en) | Insect and mite killing composition containing etoxazole and spirotetramat | |
| CN104521980A (en) | Pymetrozine-containing insecticidal composition | |
| CN104814031B (en) | A kind of Pesticidal combination containing butene-fipronil and clothianidin | |
| CN107668078A (en) | A kind of Synergistic insecticidal composition comprising chenopodium ambrosiodies essential oil and its application | |
| CN103931621B (en) | A kind of containing Tolfenpyrad with the composition pesticide of cyflumetofen | |
| CN105941423B (en) | A kind of Pesticidal combination containing capillary and cyromazine | |
| CN104082322B (en) | A kind of containing Nylar with the insecticide acaricide composition of Bifenazate | |
| CN104115833A (en) | Insect and mite killing composition containing pyriproxyfen and cyflumetofen | |
| CN103766374A (en) | Weeding composition containing flucarbazone sodium and tralkoxydim | |
| CN106973949B (en) | A kind of insecticidal composition comprising kudzu extract and matrine and use thereof | |
| CN106614696B (en) | A kind of Pesticidal combination containing Biphenthrin and its application | |
| CN107114392A (en) | Bio-insecticidal composition comprising destruxins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |